Affinage

UBE2K

Ubiquitin-conjugating enzyme E2 K · UniProt P61086

Length
200 aa
Mass
22.4 kDa
Annotated
2026-04-28
55 papers in source corpus 20 papers cited in narrative 20 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

UBE2K is a class II E2 ubiquitin-conjugating enzyme that autonomously synthesizes K48-linked polyubiquitin chains through a monomeric mechanism, with its C-terminal UBA domain enhancing chain formation rate and conferring preferential binding to K63-linked ubiquitin to facilitate assembly of K48/K63-branched chains important for proteostasis (PMID:26592444, PMID:33576509, PMID:25799589). UBE2K partners with diverse RING-finger E3 ligases, including RNF2 and yeast San1, to ubiquitinate substrates such as histone H3, p53, cyclin B1, Smac/DIABLO, mutant SOD1, Foxp3, and STUB1, targeting them for proteasomal degradation (PMID:11513855, PMID:27405755, PMID:32451438, PMID:34856358, PMID:40464424, PMID:41435988). Through these substrate-directed activities, UBE2K regulates nuclear protein quality control, neurogenic differentiation via histone H3 turnover and H3K9me3 modulation, cell cycle checkpoint recovery, apoptosis suppression, NF-κB immune signaling, and regulatory T cell homeostasis (PMID:32451438, PMID:23933584, PMID:20965177, PMID:26707646, PMID:40464424). In the original yeast system, the ortholog UBC1 is essential redundantly with UBC4/UBC5 for degradation of short-lived and abnormal proteins and for cell growth (PMID:2265617).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1990 High

    Before characterization, it was unknown whether UBC1 contributed a specific degradation function; gene disruption in yeast established that UBC1 (the UBE2K ortholog) mediates selective turnover of short-lived and abnormal proteins and is essential for viability redundantly with UBC4/UBC5.

    Evidence Gene disruption and genetic epistasis in S. cerevisiae

    PMID:2265617

    Open questions at the time
    • Mammalian substrate specificity unknown
    • Mechanism of chain type selectivity not addressed
  2. 2001 High

    The identity of E3 partners for UBE2K was unknown; yeast two-hybrid and Co-IP experiments demonstrated that UBE2K physically interacts with the RING domain of RNF2, establishing it as a cognate E2 for RING-finger E3 ligases.

    Evidence Yeast two-hybrid, reciprocal Co-IP, RING mutagenesis, in vitro ubiquitin ligase assay

    PMID:11513855

    Open questions at the time
    • Breadth of E3 partners beyond RNF2 not known
    • In vivo substrates of the UBE2K-RNF2 pair unidentified
  3. 2004 High

    It was unclear how the unusual UBA domain of UBE2K contributed to chain assembly; NMR structural analysis revealed the UBA domain connected by a flexible tether interacts with ubiquitin in a regioselective manner and facilitates polyubiquitin chain formation.

    Evidence NMR solution structure and chemical shift perturbation experiments

    PMID:15328341

    Open questions at the time
    • Chain linkage specificity not yet defined
    • Structural basis of acceptor ubiquitin positioning unresolved
  4. 2010 Medium

    Whether UBE2K had anti-apoptotic roles in mammalian cells was unknown; Co-IP and degradation assays showed UBE2K interacts with cyclin B1 and Smac/DIABLO, promoting their proteasomal degradation and blocking cell death.

    Evidence Co-immunoprecipitation and proteasome-dependent degradation assays with cell death readouts

    PMID:20537984 PMID:20965177

    Open questions at the time
    • E3 ligase(s) mediating cyclin B1 and Smac degradation not identified
    • Single-lab studies without independent replication
  5. 2013 Medium

    The mechanism by which cells overcome radiation-induced G2/M arrest was unclear with respect to UBE2K; experiments showed UBE2K interacts with p53 and targets it for proteasomal degradation, thereby activating cdc2-cyclin B1 kinase and promoting mitotic re-entry.

    Evidence BiFC, Co-IP, flow cytometry, live-cell imaging in irradiated cells

    PMID:23933584

    Open questions at the time
    • E3 partner for p53 ubiquitination by UBE2K not defined
    • Relationship to canonical MDM2-mediated p53 degradation unclear
  6. 2015 High

    The structural basis for K48 linkage specificity was unresolved; crystal structures of UBE2K~ubiquitin conjugates and biophysical analyses established that UBE2K operates as a monomer in an open/backbent conformation, with the UBA domain enhancing di-ubiquitin synthesis ~5-fold and positioning the acceptor ubiquitin for K48-specific chain elongation.

    Evidence Crystal structures, AUC, SAXS, mutagenesis, in vitro chain synthesis comparing full-length vs. UBA-deleted UBE2K

    PMID:25799589 PMID:26592444

    Open questions at the time
    • How E3 ligases modulate the monomeric chain-building mechanism not addressed
    • No in vivo confirmation of UBA-dependent chain enhancement
  7. 2015 Medium

    Whether UBE2K had roles in innate immunity was unknown; RNAi of the Drosophila ortholog UbcD4 and of UBE2K in human cells demonstrated requirement for NF-κB pathway activation downstream of IMD/TNFα/LPS signaling.

    Evidence RNAi knockdown in Drosophila and human cells, immunoblot pathway readouts

    PMID:26707646

    Open questions at the time
    • Direct ubiquitination target within NF-κB cascade not identified
    • Mechanism of action in NF-κB signaling unclear
  8. 2016 High

    It was unknown which E2 partnered with the nuclear protein quality control E3 San1; reconstituted ubiquitylation kinetics showed San1 preferentially uses Ubc1/UBE2K over Cdc34 for misfolded protein substrates.

    Evidence In vitro reconstitution with synthetic misfolded substrates, kinetic measurements

    PMID:27405755

    Open questions at the time
    • Whether this preference extends to mammalian San1 orthologs untested
    • In vivo validation limited
  9. 2018 High

    How UBE2K elongates chains beyond di-ubiquitin without an E3 was structurally unresolved; a crystal structure of UBE2K bound to K48-linked di-ubiquitin revealed that an auxiliary UBE2K molecule promotes discharge to synthesize tri-ubiquitin.

    Evidence Crystal structure at 2.47 Å, biochemical chain synthesis assays

    PMID:30336976

    Open questions at the time
    • Physiological relevance of E3-independent chain elongation in vivo not established
  10. 2020 High

    Whether UBE2K regulated chromatin during differentiation was unknown; knockdown in human ESCs and C. elegans showed UBE2K ubiquitinates histone H3 for K48-linked proteasomal degradation, and its loss upregulates SETDB1, causing H3K9me3-mediated repression of neurogenic genes.

    Evidence shRNA/RNAi knockdown, Co-IP, ubiquitination assays, hESC differentiation, C. elegans genetics

    PMID:32451438

    Open questions at the time
    • How UBE2K selectively targets histone H3 over other histones is unexplained
    • E3 ligase for H3 ubiquitination not identified
  11. 2021 High

    The chain topology produced by UBE2K in the context of mixed-linkage ubiquitin was unclear; NMR, proteomics, and genetics demonstrated the UBA domain preferentially binds K63-linked chains and facilitates K48/K63-branched conjugate assembly, linking this topology to proteostasis.

    Evidence NMR, in vitro ubiquitination, yeast genetic epistasis, mass spectrometry

    PMID:33576509

    Open questions at the time
    • Identity of in vivo substrates receiving branched chains from UBE2K unknown
    • Quantitative contribution to total branched ubiquitin pool not measured
  12. 2021 High

    No selective small-molecule or protein-based inhibitors of UBE2K existed; engineered ubiquitin variants were shown to bind a hydrophobic cleft on UBE2K and inhibit both E1 charging and E3-mediated transfer by steric and allosteric mechanisms.

    Evidence Crystal structure of UbV-UBE2K complex, mutagenesis, in vitro charging and transfer assays

    PMID:34397214

    Open questions at the time
    • In vivo efficacy and selectivity of UbV inhibitors not tested
  13. 2021 Medium

    Whether UBE2K contributed to clearance of ALS-linked mutant SOD1 was unknown; Co-IP and linkage-specific ubiquitination assays showed UBE2K selectively binds and K48-polyubiquitinates mutant (not wild-type) SOD1, promoting its proteasomal degradation and protecting against proteotoxicity.

    Evidence Co-IP, K48-specific ubiquitination assay, transgenic mouse spinal cord, cell viability assay

    PMID:34856358

    Open questions at the time
    • E3 ligase mediating mutant SOD1 ubiquitination with UBE2K not identified
    • Single-lab finding awaiting independent replication
  14. 2025 Medium

    New substrate-level roles and regulatory inputs for UBE2K continue to emerge: UBE2K ubiquitinates STUB1/CHIP in breast cancer cells within a CREB1-driven positive feedback loop, and UBE2K-mediated Foxp3 ubiquitination in Tregs is modulated by the gut microbial protein Amuc_1409.

    Evidence Co-IP, ubiquitination assays, ChIP, dual-luciferase reporter, mouse Treg models

    PMID:40464424 PMID:41435988

    Open questions at the time
    • Whether UBE2K acts directly or through an E3 for Foxp3 ubiquitination is unresolved
    • Physiological relevance of UBE2K-STUB1 axis beyond breast cancer cell lines not established
    • Feedback loop dynamics not modeled quantitatively

Open questions

Synthesis pass · forward-looking unresolved questions
  • A systematic map of E3 ligase partners and substrates of UBE2K in vivo, the structural basis of E3-dependent substrate selection, and the relative contributions of linear vs. branched chain building to UBE2K's physiological functions remain to be established.
  • No comprehensive E3 partner catalog exists
  • No structural model of UBE2K within an E3-substrate ternary complex
  • In vivo phenotypes in mammalian knockout models not systematically characterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 8 GO:0016874 ligase activity 3
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-392499 Metabolism of proteins 9 R-HSA-1640170 Cell Cycle 2 R-HSA-168256 Immune System 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-4839726 Chromatin organization 1
Partners
CCNB1DIABLOFOXP3H3RNF2SOD1STUB1TP53

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 Yeast UBC1 (ortholog of UBE2K) is a ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins, functioning redundantly with UBC4 and UBC5 in an essential subfamily required for cell growth and viability. Gene disruption, genetic epistasis, growth phenotype analysis The EMBO journal High 2265617
2004 UBE2K/Ubc1 is a class II E2 enzyme with a C-terminal ubiquitin-associated (UBA) domain connected to the catalytic domain by a flexible 22-residue tether; the UBA domain interacts with ubiquitin in a regioselective manner and facilitates polyubiquitin chain formation. NMR solution structure, NMR chemical shift perturbation experiments The Journal of biological chemistry High 15328341
2001 UBE2K (Hip-2) physically interacts with RING finger protein RNF2, and this interaction requires the RING domain of RNF2; RNF2 exhibits E3 ubiquitin ligase activity in the presence of UBE2K, establishing UBE2K as the cognate E2 for a subset of RING finger E3 ligases. Yeast two-hybrid screen, in vitro and in vivo co-immunoprecipitation, RING domain mutagenesis, ubiquitin ligase activity assay FEBS letters High 11513855
2015 UBE2K preferentially catalyses formation of Lys48-linked ubiquitin chains via a monomeric mechanism; crystal structure of the UBE2K~ubiquitin conjugate reveals key features including acceptor ubiquitin positioning required for K48-specific chain synthesis. Crystal structure determination, site-directed mutagenesis, in vitro ubiquitin chain synthesis assay, structural modeling Scientific reports High 26592444
2015 The HIP2 (UBE2K)~ubiquitin thioester conjugate remains predominantly monomeric in solution and adopts an open/backbent conformation; the UBA domain enhances di-ubiquitin formation rate approximately 5-fold compared to UBE2K lacking the UBA domain. Analytical ultracentrifugation, SAXS, stable disulfide complex analysis, in vitro activity assays comparing full-length vs. UBA-deleted UBE2K PloS one High 25799589
2018 Crystal structure of UBE2K/E2-25K in complex with K48-linked di-ubiquitin (at 2.47 Å) reveals that interaction between acceptor di-ubiquitin and auxiliary UBE2K promotes discharge reaction and K48-linked tri-ubiquitin synthesis without an E3 ligase. Crystal structure determination, biochemical ubiquitin chain synthesis assays Biochemical and biophysical research communications High 30336976
2021 The UBA domain of UBE2K (and yeast Ubc1) preferentially binds K63-linked ubiquitin chains and facilitates assembly of K48/K63-branched ubiquitin conjugates; this branched chain topology is linked to maintenance of cellular proteostasis. NMR, structural modeling, in vitro ubiquitination assays, genetic epistasis in yeast, mass spectrometry proteomics The EMBO journal High 33576509
2021 Ubiquitin variants (UbVs) bind UBE2K at a hydrophobic cleft distinct from the active site and inhibit both ubiquitin charging of UBE2K and E3-catalyzed ubiquitin transfer, likely by steric clash with E1 and allosteric disruption of E3 interactions. Crystal structure of UbV-UBE2K complex, in vitro charging and transfer assays, mutagenesis ACS chemical biology High 34397214
2020 UBE2K in human embryonic stem cells binds histone H3 and induces its K48-linked polyubiquitination and proteasomal degradation; loss of UBE2K upregulates SETDB1, causing H3K9 trimethylation and repression of neurogenic genes during differentiation. The worm ortholog ubc-20 similarly regulates H3 levels and H3K9me3 in C. elegans germ cells. RNAi/shRNA knockdown, co-immunoprecipitation, ubiquitination assays, Western blot, differentiation assays in hESCs, C. elegans genetics Communications biology High 32451438
2013 Hip2 (UBE2K) overcomes radiation-induced G2/M arrest by interacting with p53 and targeting it for ubiquitin-proteasome-mediated degradation, thereby activating cdc2-cyclin B1 kinase to promote mitotic entry. Bimolecular fluorescence complementation, co-immunoprecipitation, flow cytometry, live cell fluorescence imaging, overexpression studies Biochimica et biophysica acta Medium 23933584
2010 Hip2 (UBE2K) interacts with cyclin B1 and promotes its degradation through the ubiquitin proteasome pathway, thereby blocking cyclin B1-mediated cell death. Co-immunoprecipitation, ubiquitin proteasome degradation assay, cell death assay FEBS letters Medium 20965177
2010 Hip2 (UBE2K) interacts with and promotes proteasomal degradation of the pro-apoptotic protein Smac/DIABLO, blocking Smac-mediated cell death. Co-immunoprecipitation (in vivo and in vitro), ubiquitin proteasome degradation assay, cell death assay Biochemical and biophysical research communications Medium 20537984
2018 Hip2 (UBE2K) is phosphorylated after UV radiation, and this phosphorylation inhibits p53 to suppress p21 expression and allow cell cycle re-entry from G1/S arrest. pIMAGO phosphorylation assay, thymidine synchronization, Western blot, flow cytometry Genes & genomics Low 30264212
2021 HIP2 (UBE2K) interacts specifically with mutant (not wild-type) SOD1, is upregulated in response to mutant SOD1 expression, and modifies mutant SOD1 via K48-linked polyubiquitination leading to UPS-mediated degradation, protecting cells from mutant SOD1 proteotoxicity. Co-immunoprecipitation, ubiquitination assay with linkage-specific antibodies, Western blot in transgenic mouse spinal cord, cell viability assay Biochimica et biophysica acta. Molecular basis of disease Medium 34856358
2015 UbcD4, the Drosophila ortholog of UBE2K/E2-25K, is required for activation of the IMD (NF-κB) immune deficiency pathway; RNAi knockdown of UBE2K in human cells similarly inhibited TNFα- and LPS-mediated NF-κB pathway activation. RNAi knockdown in Drosophila, immunoblot-based pathway activity assay, RNAi knockdown in human cells Biochemical and biophysical research communications Medium 26707646
2016 The E3 ligase San1 functions preferentially with yeast Ubc1 (ortholog of UBE2K) over Cdc34 for ubiquitylation of misfolded protein substrates during nuclear protein quality control, as demonstrated by reconstituted in vitro ubiquitylation kinetics. In vitro reconstitution of San1-mediated ubiquitylation, kinetic measurements with synthetic misfolded substrate peptide, competition assays The Journal of biological chemistry High 27405755
2025 A microsporidial otubain deubiquitinase (ehOTUB1) selectively binds UBE2K and inhibits its ubiquitin conjugation by sterically blocking UBE2K docking onto the E1 adenylation site, preventing ubiquitin transfer to UBE2K independent of DUB catalytic activity. In vitro binding assays, ubiquitin conjugation/charging assays, structural modeling, mutagenesis bioRxivpreprint Medium 40766534
2025 UBE2K promotes ubiquitination and proteasomal degradation of STUB1 (CHIP) in breast cancer cells, activating the PKA/CREB1 signaling pathway; CREB1 in turn transcriptionally activates UBE2K, forming a positive feedback loop. Co-immunoprecipitation, ubiquitination assay, ChIP assay, dual-luciferase reporter assay, Western blot, functional rescue experiments Biochimica et biophysica acta. Molecular basis of disease Medium 41435988
2025 Amuc_1409 (an Akkermansia muciniphila protein) interacts with UBE2K to reduce ubiquitination-mediated degradation of Foxp3 in regulatory T cells, promoting Treg differentiation and IL-10 production to suppress inflammatory responses. Co-immunoprecipitation, ubiquitination assay, Foxp3-DTR and IL-10-KO mouse models, cell differentiation assays Advanced science Medium 40464424
2024 Circular RNA circ-UBE2K binds HNRNPU to form a complex that upregulates expression of the parental gene UBE2K, leading to abnormal microglial activation and neuroinflammation promoting major depressive disorder. Pull-down mass spectrometry, RNA immunoprecipitation (RIP), FISH, lentiviral/AAV overexpression and knockdown in mouse hippocampus, behavioral tests Theranostics Medium 38994030

Source papers

Stage 0 corpus · 55 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1990 UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation. The EMBO journal 150 2265617
2020 E2 conjugases UBC1 and UBC2 regulate MYB42-mediated SOS pathway in response to salt stress in Arabidopsis. The New phytologist 76 32167578
2015 The molecular basis of lysine 48 ubiquitin chain synthesis by Ube2K. Scientific reports 50 26592444
2004 Solution structure of the flexible class II ubiquitin-conjugating enzyme Ubc1 provides insights for polyubiquitin chain assembly. The Journal of biological chemistry 49 15328341
1993 Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana. The Plant journal : for cell and molecular biology 46 8220461
2007 Roscovitine-activated HIP2 kinase induces phosphorylation of wt p53 at Ser-46 in human MCF-7 breast cancer cells. Journal of cellular biochemistry 43 17203463
2017 Over-expression of tobacco UBC1 encoding a ubiquitin-conjugating enzyme increases cadmium tolerance by activating the 20S/26S proteasome and by decreasing Cd accumulation and oxidative stress in tobacco (Nicotiana tabacum). Plant molecular biology 41 28508171
2001 E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme. FEBS letters 41 11513855
2000 Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. The Journal of biological chemistry 36 11134048
2021 The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains. The EMBO journal 32 33576509
1997 Isolation of a cDNA coding for an ubiquitin-conjugating enzyme UBC1 of tomato--the first stress-induced UBC of higher plants. FEBS letters 32 9202147
2020 The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. Communications biology 30 32451438
2013 Interaction of the microtubule-associated host protein HIP2 with viral helper component proteinase is important in infection with potato virus A. Molecular plant-microbe interactions : MPMI 29 23489059
2024 Microglial circ-UBE2K exacerbates depression by regulating parental gene UBE2K via targeting HNRNPU. Theranostics 21 38994030
2018 Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models. Cell death & disease 21 30282965
2008 HIP2: an online database of human plasma proteins from healthy individuals. BMC medical genomics 21 18439290
2019 Blood and brain protein levels of ubiquitin-conjugating enzyme E2K (UBE2K) are elevated in individuals with schizophrenia. Journal of psychiatric research 18 30901725
2018 Long noncoding RNA linc-UBC1 promotes tumor invasion and metastasis by regulating EZH2 and repressing E-cadherin in esophageal squamous cell carcinoma. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 17 29552776
2021 Identification of Ubiquitin Variants That Inhibit the E2 Ubiquitin Conjugating Enzyme, Ube2k. ACS chemical biology 16 34397214
2019 Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development. Acta histochemica 14 31047684
2018 Crystal structure of the Ube2K/E2-25K and K48-linked di-ubiquitin complex provides structural insight into the mechanism of K48-specific ubiquitin chain synthesis. Biochemical and biophysical research communications 14 30336976
2013 Hip2 ubiquitin-conjugating enzyme overcomes radiation-induced G2/M arrest. Biochimica et biophysica acta 14 23933584
1997 Biochemical characterization of Caenorhabditis elegans UBC-1: self-association and auto-ubiquitination of a RAD6-like ubiquitin-conjugating enzyme in vitro. The Biochemical journal 14 9359401
2018 Hip2 ubiquitin-conjugating enzyme has a role in UV-induced G1/S arrest and re-entry. Genes & genomics 13 30264212
2010 Hip2 interacts with cyclin B1 and promotes its degradation through the ubiquitin proteasome pathway. FEBS letters 13 20965177
2016 The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control. The Journal of biological chemistry 12 27405755
2004 NUB1-mediated targeting of the ubiquitin precursor UbC1 for its C-terminal hydrolysis. European journal of biochemistry 12 15009209
2023 UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma. International journal of oncology 11 36896783
2015 UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila. Biochemical and biophysical research communications 11 26707646
1995 Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes. DNA and cell biology 11 7546294
2020 Reduction of Hip2 suppresses gastric cancer cell proliferation, migration, invasion and tumorigenesis. Translational cancer research 10 35117423
2022 UBE2K promotes the malignant progression of hepatocellular carcinoma by regulating c-Myc. Biochemical and biophysical research communications 9 36481361
2021 Ixazomib inhibits myeloma cell proliferation by targeting UBE2K. Biochemical and biophysical research communications 9 33647537
2010 Hip2 interacts with and destabilizes Smac/DIABLO. Biochemical and biophysical research communications 9 20537984
2021 The E2 ubiquitin-conjugating enzyme HIP2 is a crucial regulator of quality control against mutant SOD1 proteotoxicity. Biochimica et biophysica acta. Molecular basis of disease 8 34856358
2025 Akkermansia Muciniphila Alleviates Severe Acute Pancreatitis via Amuc1409-Ube2k-Foxp3 Axis in Regulatory T Cells. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 7 40464424
2023 Hypoxia-induced UBE2K promotes the malignant progression of HCC. Pathology, research and practice 7 37003132
2022 Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1 (yotari) Mice. International journal of molecular sciences 7 35216141
2015 The HIP2~ubiquitin conjugate forms a non-compact monomeric thioester during di-ubiquitin synthesis. PloS one 7 25799589
1999 Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in the developing mouse brain. Journal of chemical neuroanatomy 7 10585161
2022 Ubiquitin-Conjugating Enzymes Ubc1 and Ubc4 Mediate the Turnover of Hap4, a Master Regulator of Mitochondrial Biogenesis in Saccharomyces cerevisiae. Microorganisms 6 36557625
2011 Tolerance to thermal and reductive stress in Saccharomyces cerevisiae is amenable to regulation by phosphorylation-dephosphorylation of ubiquitin conjugating enzyme 1 (Ubc1) S97 and S115. Yeast (Chichester, England) 6 21996927
2020 Transcription factor ZNF703 activates linc-UBC1 to stimulate the progression of glioma. European review for medical and pharmacological sciences 4 32271436
2016 The ubiquitin-conjugating enzyme, Ubc1, indirectly regulates SNF1 kinase activity via Forkhead-dependent transcription. Microbial cell (Graz, Austria) 3 28357323
2024 Silencing UBE2K inhibits the growth of glioma cells by inducing the autophagy-related apoptosis. Journal of biochemical and molecular toxicology 2 38963134
2021 Ubc1 turnover contributes to the spindle assembly checkpoint in Saccharomyces cerevisiae. G3 (Bethesda, Md.) 2 34586382
2020 Linc-UBC1 stimulates the metastasis and progression of ovarian cancer via downregulating p53 level. European review for medical and pharmacological sciences 2 32096175
2022 Leishmania infantum UBC1 in Metacyclic Promastigotes from Phlebotomus perniciosus, a Vaccine Candidate for Zoonotic Visceral Leishmaniasis. Vaccines 1 35214689
2000 YAC/BAC-based physical and transcript mapping around the gracile axonal dystrophy (gad) locus identifies Uchl1, Pmx2b, Atp3a2, and Hip2 genes. Genomics 1 10873389
2025 UBE2K Facilitates Erlotinib Resistance and Induces Epithelial Mesenchymal Transition in Osteosarcoma Cancer Stem-Like Cells via Activating the mTOR Signaling. Pharmacology 0 40159320
2025 A microsporidial deubiquitinase blocks ubiquitin transfer from adenylated E1 to human UBE2K ubiquitin conjugating enzyme. bioRxiv : the preprint server for biology 0 40766534
2025 UBE2K promotes breast cancer growth by ubiquitinating and degrading STUB1 to regulate the PKA/CREB1 signaling pathway, forming a feedback loop. Biochimica et biophysica acta. Molecular basis of disease 0 41435988
2024 Simulation and Computational Study of RING Domain Mutants of BRCA1 and Ube2k in AD/PD Pathophysiology. Molecular biotechnology 0 38172369
2024 Erratum to reduction of Hip2 suppresses gastric cancer cell proliferation, migration, invasion and tumorigenesis. Translational cancer research 0 39430844
2022 Characterization of the chimeric protein cUBC1 engineered by substituting the linker of E2-25K into UBC1 enzyme of Saccharomyces cerevisiae. International journal of biological macromolecules 0 35429515