Affinage

UBA52

Ubiquitin-ribosomal protein eL40 fusion protein · UniProt P62987

Length
128 aa
Mass
14.7 kDa
Annotated
2026-04-28
30 papers in source corpus 23 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

UBA52 encodes a ubiquitin–RPL40 fusion protein whose proteolytic cleavage simultaneously supplies free ubiquitin to the cellular ubiquitin pool and the ribosomal protein RPL40 to the 60S ribosomal subunit, linking ubiquitin homeostasis to translational control (PMID:27829658, PMID:39193909). The ubiquitin moiety participates in K48-, K63-, K11-, and K27-linked polyubiquitination of diverse substrates—including HSP90, VDAC1, CCNB1, ferroportin, DRP1, Daxx, and SREBF1—through cooperation with E3 ligases such as CHIP and APC11, thereby regulating mitochondrial integrity, cell cycle progression, ferroptosis, autophagy, and lipid metabolism (PMID:36497031, PMID:36755387, PMID:31814919, PMID:38352945, PMID:40615369, PMID:41553582, PMID:41608635). The RPL40 fragment is trimethylated at K22 by SMYD5, a modification required for ribosome elongation fidelity and polysome maintenance; RPL40 also inhibits RNF168-mediated H2A/H2AX ubiquitination at DNA damage sites by masking the nucleosome acidic patch, thereby limiting 53BP1 recruitment and modulating DNA repair (PMID:39048817, PMID:40184250, PMID:37451480). Biallelic disruption of Uba52 arrests porcine embryos at the 4-cell stage, establishing its essential role in preimplantation development (PMID:30135083).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2002 Medium

    Before transcriptional regulation of UBA52 was characterized, it was unknown how its expression responded to metabolic cues; promoter analysis revealed functional glucose-responsive E-box and stress-response elements that drive UBA52 upregulation under high-glucose conditions, establishing UBA52 as a metabolically regulated gene.

    Evidence Promoter deletion/mutagenesis with SEAP reporter, in situ hybridization in diabetic mouse kidneys, high-glucose MDCK cell culture

    PMID:12171997

    Open questions at the time
    • Upstream transcription factors binding E-box/STRE not identified
    • Functional consequences of glucose-induced UBA52 upregulation on ubiquitin pool or ribosome function not tested
  2. 2016 High

    The fundamental question of whether UBA52 cleavage is required to simultaneously supply both free ubiquitin and a functional ribosomal protein was answered: Uba52 knockout mice showed that RPL40 alone cannot maintain cyclin D expression or protein synthesis, and UBA52 re-expression rescued both defects, establishing the dual-product fusion as essential for ribosome function and cell cycle progression.

    Evidence Uba52 knockout mouse, ribosomal fractionation, protein synthesis assay, overexpression rescue, cell cycle analysis

    PMID:27829658

    Open questions at the time
    • Identity of the protease(s) cleaving UBA52 not determined
    • Relative contributions of UBA52 vs RPS27A to the cellular ubiquitin pool not quantified
  3. 2018 High

    Whether UBA52 is required for early embryonic development was tested by CRISPR knockout in porcine oocytes, revealing arrest at the 4-cell stage coinciding with zygotic genome activation, demonstrating an essential developmental role beyond cultured cell phenotypes.

    Evidence CRISPR/Cas9 biallelic disruption in porcine oocytes, IVF, embryo culture, immunolabeling, Western blotting

    PMID:30135083

    Open questions at the time
    • Whether arrest reflects ubiquitin depletion, RPL40 loss, or both is unresolved
    • Not tested in mammalian species beyond pig
  4. 2018 Medium

    UBA52 was identified as a host factor for influenza A virus replication; its knockdown reduced H5N1 viral titers and proinflammatory cytokine production, extending UBA52's roles beyond housekeeping to host-pathogen interactions.

    Evidence IP-MS identification of UBA52–PA protein interaction in chicken cells, UBA52 knockdown with viral titer and cytokine measurement

    PMID:29867845

    Open questions at the time
    • Whether the ubiquitin or RPL40 moiety mediates the proviral effect is unknown
    • No reciprocal validation or reconstitution of the interaction
  5. 2019 Medium

    Two studies addressed UBA52's role in cell cycle control: one showed UBA52 promotes APC11-dependent ubiquitination and degradation of cyclin B1 for mitotic exit, and another linked lncRNA LUCAT1 binding of UBA52 to suppression of the RPL40–MDM2–p53 axis, revealing UBA52 as a node integrating ubiquitin-mediated proteolysis and ribosomal protein-mediated p53 surveillance.

    Evidence Co-IP, shRNA/siRNA epistasis for APC11–CCNB1 axis; ChIRP-MS and RIP for LUCAT1–UBA52 binding with p53 pathway readouts

    PMID:30690837 PMID:31814919

    Open questions at the time
    • Direct ubiquitin transfer from UBA52-derived ubiquitin to CCNB1 not reconstituted in vitro
    • LUCAT1–UBA52 mechanism of p53 suppression is indirect
  6. 2022 High

    The question of whether UBA52-derived ubiquitin participates in specific linkage-type ubiquitination was answered: in vitro ubiquitylation assays with K63 mutants showed that UBA52 K63 is required for CHIP-mediated K63-linked ubiquitination of HSP90, and UBA52 co-localizes with α-synuclein at mitochondria, placing it in Parkinson's disease-relevant pathways.

    Evidence In vitro ubiquitylation with K63R mutant, Co-IP, mass spectrometry, confocal co-localization in neuronal PD models

    PMID:36497031

    Open questions at the time
    • Functional consequence of HSP90 K63-ubiquitination not determined
    • Whether UBA52 preferentially supplies K63-linked chains versus other linkages in vivo is unclear
  7. 2023 High

    Multiple studies in 2023 resolved distinct functions of the two UBA52-derived products: the ubiquitin moiety mediates CHIP-dependent ubiquitination of VDAC1 to protect mitochondrial integrity and drives ubiquitination of ferroportin to regulate iron homeostasis, while the RPL40 fragment was shown to be recruited to DNA damage sites where it inhibits RNF168-mediated H2A ubiquitination by masking the nucleosome acidic patch.

    Evidence In vitro ubiquitylation of VDAC1 via CHIP with mitochondrial functional readouts; UBA52–Fpn ubiquitination assay with competitive hydralazine binding and nerve injury model; laser micro-irradiation with RPL40 fragment domain dissection and H2A ubiquitination assay

    PMID:36755387 PMID:37451480 PMID:38352945

    Open questions at the time
    • How RPL40 is released from the ribosome to act at DNA damage sites is unknown
    • Whether CHIP is the sole E3 for UBA52-mediated VDAC1 ubiquitination in vivo is unresolved
    • Identity of the E3 ligase mediating UBA52-dependent Fpn ubiquitination not established
  8. 2023 Medium

    The relationship between RPL40 and p53 stabilization was refined: RPL40 knockdown alone does not stabilize p53 (unlike most ribosomal proteins), but combined loss of RPL40 and RPS27a does, revealing functional redundancy between the two ubiquitin-fusion ribosomal proteins in the nucleolar stress–p53 pathway while confirming both are individually required for rRNA production.

    Evidence siRNA knockdown across multiple cell lines (U2OS, MCF7, LNCaP), p53 Western blotting, rRNA production assays

    PMID:37371478

    Open questions at the time
    • Whether redundancy reflects shared ubiquitin supply or shared ribosomal function is unresolved
    • Cell-type specificity mechanisms not explained
  9. 2024 High

    A major advance identified RPL40 K22 trimethylation by SMYD5 as a functionally important ribosomal modification: two independent groups showed SMYD5 is the sole methyltransferase for RPL40 K22me3, and its loss causes ribosome collisions and decreased polysome levels, establishing post-translational modification of the RPL40 fragment as a regulator of translation elongation fidelity.

    Evidence In vitro methyltransferase assay with recombinant SMYD5 and active-site mutants, SMYD5 KO cells with MS quantification, polysome profiling, ribosome collision assay, PDX xenograft models

    PMID:39048817 PMID:39103523 PMID:40184250

    Open questions at the time
    • Which specific mRNAs are most affected by K22me3 loss is not fully catalogued
    • Whether K22me3 affects RPL40's non-ribosomal functions (e.g., at DNA damage sites) is untested
  10. 2024 High

    MLKL was identified as a physiological regulator of UBA52 processing: MLKL binds UBA52 and promotes its cleavage via USP7, and Mlkl knockout brains show reduced free ubiquitin, decreased K63-linked polyubiquitination of autophagy regulators BECN1 and ULK1, and impaired autophagy leading to neurodegeneration, placing UBA52 cleavage under necroptosis-independent MLKL control.

    Evidence Reciprocal Co-IP, mlkl KO mice with ubiquitin level quantification, USP7 deubiquitinase assay, K63 polyubiquitination analysis, snRNA-seq, behavioral tests

    PMID:39193909

    Open questions at the time
    • Whether USP7 directly cleaves the UBA52 fusion or acts indirectly is not fully resolved
    • MLKL-independent UBA52 processing pathways not characterized
  11. 2025 Medium

    UBA52-derived ubiquitin was shown to mediate ubiquitination of additional substrates—DRP1 (competed by ROCK2 to regulate mitochondrial fission) and MCT1 (K63-linked, stabilizing the transporter)—and Sparc was identified as an upstream interactor that upregulates UBA52 expression in microglia for anti-inflammatory neuroprotection, broadening the substrate repertoire and upstream regulatory network.

    Evidence Co-IP and ubiquitination assays for ROCK2/DRP1/UBA52 axis in cholangiocarcinoma; proteomic and Co-IP for EMMPRIN–UBA52–MCT1 axis with hepatocyte-specific KO mice; IP-MS and siRNA epistasis for Sparc–UBA52 in microglia

    PMID:40615369 PMID:40917064 PMID:41660506

    Open questions at the time
    • E3 ligases mediating UBA52-dependent DRP1 and MCT1 ubiquitination not identified
    • Whether these represent UBA52-specific ubiquitin or general pool ubiquitin is unclear
  12. 2026 Medium

    UBA52 was placed upstream of the pro-apoptotic protein Daxx: UBA52 overexpression promotes Daxx ubiquitination and degradation, protecting neurons from intracerebral hemorrhage-induced apoptosis and mitochondrial dysfunction, and NUDT7 was shown to target UBA52 itself for proteasomal degradation, reducing K11/K27/K48-linked ubiquitination of SREBF1 and promoting lipogenesis during PRRSV infection.

    Evidence IP-LC/MS and Co-IP for UBA52–Daxx with AAV9 overexpression in ICH mouse model and Daxx rescue epistasis; Co-IP and linkage-specific ubiquitination assay for NUDT7–UBA52–SREBF1 axis with viral replication readout

    PMID:41553582 PMID:41608635

    Open questions at the time
    • E3 ligase for Daxx ubiquitination downstream of UBA52 not identified
    • How NUDT7 (a nudix hydrolase) promotes UBA52 degradation mechanistically is unclear
    • Single-lab findings for both axes

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: (1) the identity and regulation of the protease(s) responsible for UBA52 fusion cleavage under physiological conditions; (2) whether specific substrates receive ubiquitin preferentially from UBA52-derived pools versus other ubiquitin genes; (3) how RPL40 is released from assembled ribosomes to perform non-ribosomal functions at DNA damage sites and during viral infection; and (4) the structural basis for RPL40 repositioning on the 40S subunit during stress.
  • No protease for UBA52 cleavage has been biochemically identified
  • No method distinguishes UBA52-derived ubiquitin from other ubiquitin gene products in vivo
  • Structural basis for RPL40 repositioning to the 40S subunit awaits peer-reviewed validation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0031386 protein tag activity 7 GO:0005198 structural molecule activity 3 GO:0042393 histone binding 1
Localization
GO:0005840 ribosome 3 GO:0005739 mitochondrion 2 GO:0005829 cytosol 2 GO:0005634 nucleus 1
Pathway
R-HSA-392499 Metabolism of proteins 9 R-HSA-5357801 Programmed Cell Death 3 R-HSA-1640170 Cell Cycle 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-9612973 Autophagy 2 R-HSA-73894 DNA Repair 1
Partners
APC11CHIPMLKLNUDT7RNF168SMYD5USP7VDAC1
Complex memberships
60S ribosomal subunit80S ribosome

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2016 UBA52 encodes a ubiquitin-RPL40 fusion protein that must be cleaved to simultaneously supply both RPL40 and ubiquitin to the ribosomal complex; the RPL40 portion alone is insufficient to maintain cyclin D expression or protein synthesis, and UBA52-deficient cells show decreased protein synthesis and cell-cycle arrest rescued by UBA52 overexpression. Uba52 knockout mouse model, cell-cycle analysis, protein synthesis assay, overexpression rescue, ribosomal complex fractionation/co-immunoprecipitation Scientific reports High 27829658
2018 UBA52 is essential for preimplantation embryo development; CRISPR/Cas9-mediated biallelic disruption of Uba52 in porcine oocytes arrests embryos at the 4-cell stage (around major zygotic genomic activation) and prevents blastocyst formation, with reduced UBA52 and proteasome protein levels and abnormal nuclear morphology. CRISPR/Cas9 gene editing in porcine oocytes followed by in vitro fertilization, embryo culture, immunolabeling, Western blotting Biology open High 30135083
2019 RPL40 (the C-terminal product of UBA52 cleavage) functions in the ribosomal protein–MDM2–p53 pathway; the lncRNA LUCAT1 binds UBA52 (shown by chromatin isolation by RNA purification + mass spectrometry and RNA immunoprecipitation), suppressing this pathway and thereby inhibiting p53-mediated cell cycle arrest and apoptosis in colorectal cancer cells. ChIRP-MS, RNA immunoprecipitation, LUCAT1 knockdown with p53 pathway readouts Cancer science Medium 30690837
2019 UBA52 promotes ubiquitination-dependent degradation of CCNB1 (cyclin B1) through APC11; knockdown of APC11 prevents UBA52-driven CCNB1 degradation and causes G2/M arrest even when UBA52 is overexpressed, placing UBA52 upstream of APC11 in CCNB1 proteolysis and cell cycle progression. Co-immunoprecipitation, shRNA/siRNA knockdown, CCNB1 overexpression/knockdown, cell cycle analysis, xenograft tumor model American journal of translational research Medium 31814919
2022 UBA52 interacts with α-synuclein, HSP90, and the E3 ubiquitin ligase CHIP; the lysine-63 residue of UBA52 is required for CHIP-mediated K63-linked ubiquitylation of HSP90, as shown by in vitro ubiquitylation assay with UBA52 mutants; UBA52 also co-localizes with α-synuclein at mitochondria. In silico prediction, mass spectrometry, co-immunoprecipitation, in vitro ubiquitylation assay with K63 mutant, confocal co-localization, Myc-UBA52 overexpression in neuronal PD models Cells High 36497031
2023 UBA52 interacts with mitochondrial outer membrane channel VDAC1 and participates in CHIP-mediated ubiquitylation of VDAC1; UBA52 overexpression in neurons preserves mitochondrial membrane potential, complex I activity, cytochrome c retention, and calcium homeostasis, inhibiting apoptotic and autophagic cell death in a Parkinson's disease model. Mass spectrometry, co-immunoprecipitation, in vitro ubiquitylation assay, mitochondrial membrane potential assay (JC-1), complex I activity assay, cytochrome c fractionation, Myc-UBA52 overexpression/knockdown ACS chemical neuroscience High 36755387
2023 UBA52 (via its C-terminal L40/ribosomal fragment) interacts with H2A/H2AX histones and RNF168, is recruited to laser-induced DNA damage sites, and the L40 fragment inhibits RNF168-mediated H2A/H2AX ubiquitination at K13/K15 by masking the nucleosome acidic patch (E143/E144), thereby limiting 53BP1 recruitment and fine-tuning DNA repair. Co-immunoprecipitation, laser micro-irradiation recruitment assay, ectopic expression of ribosomal fragments, H2A/H2AX ubiquitination assay, 53BP1 recruitment assay, UBA52 knockdown The Journal of biological chemistry High 37451480
2023 RPL40 (UBA52 C-terminal product) knockdown does not stabilize p53 in U2OS cells, contrasting with most ribosomal proteins; however, combined knockdown of RPS27a and RPL40 robustly induces p53 in MCF7 and LNCaP cells. Both proteins are required for rRNA production in all cell lines tested, indicating cell-type-specific roles in p53 signaling but universal roles in ribosome biogenesis. siRNA knockdown, Western blotting for p53 stabilization, actinomycin D treatment, rRNA production assays across multiple cell lines Biomolecules Medium 37371478
2024 SMYD5 directly methyltransferases RPL40 (the ribosomal protein encoded within UBA52) at lysine 22 to produce K22me3; this modification regulates mRNA translation output and ribosome elongation fidelity (evidenced by ribosome collision upon SMYD5/K22me3 loss), and promotes oncogenic gene expression in gastric adenocarcinoma. Biochemical-proteomics substrate identification, in vitro methyltransferase assay with recombinant SMYD5, SMYD5 knockout cells (mass spectrometry for K22me3 loss), polysome profiling, ribosome collision assay, in vivo mouse GAC models and PDX xenografts Nature High 39048817 39103523
2024 SMYD5 requires a KXY motif for recognition and trimethylation of RPL40 K22; active site mutations in recombinant SMYD5 ablate this activity; SMYD5 knockout cells lose all RPL40 K22me3 and show decreased polysome levels, confirming the enzymatic requirement for translation. Systematic mass spectrometry of ribosomal methylation sites, in vitro fractionated-lysate methylation assay, recombinant SMYD5 with active site mutagenesis, SMYD5 knockout, polysome profiling, recognition motif scanning Cell reports High 40184250
2024 MLKL binds UBA52 under physiological conditions; loss of Mlkl prevents UBA52 cleavage and decreases free ubiquitin levels in brain; the deubiquitinase USP7 mediates UBA52 processing in a manner regulated by MLKL; reduced ubiquitin leads to decreased K63-linked polyubiquitination of BECN1 and ULK1, impairing autophagy and contributing to neurodegeneration. Co-immunoprecipitation (MLKL–UBA52 interaction), mlkl knockout mice (ubiquitin level measurement), USP7 deubiquitinase assay, K63 polyubiquitination analysis of BECN1/ULK1, single-nucleus RNA sequencing, cognitive behavioral tests Autophagy High 39193909
2024 Knockdown of UBA52 induces autophagy through EMC6 in hepatocellular carcinoma cells, reducing HCC cell growth and metastasis both in vitro and in vivo; UBA52 thus promotes HCC proliferation and migration by suppressing EMC6-mediated autophagy. UBA52 knockdown/overexpression, CCK-8, colony formation, wound healing, Transwell assays, subcutaneous and metastatic xenograft mouse models, bioinformatic autophagy association analysis Journal of cellular and molecular medicine Medium 38445807
2023 UBA52 drives ubiquitination and proteasomal degradation of ferroportin (Fpn) in dorsal root ganglion neurons following peripheral nerve injury; the drug hydralazine competitively binds UBA52, blocking Fpn ubiquitination, thereby reducing intracellular iron accumulation, lipid peroxidation, and neuronal ferroptosis, and accelerating axon regeneration. In vitro and in vivo ubiquitination assays, co-immunoprecipitation (UBA52–Fpn), competitive binding assay (hydralazine vs UBA52), ROS/iron/lipid peroxidation measurements, peripheral nerve injury mouse model with motor function readout Journal of pharmaceutical analysis High 38352945
2018 UBA52 interacts with H5N1 influenza A virus PA, PA-N155, and PA-N182 proteins in chicken cells (identified by immunoprecipitation/mass spectrometry); knockdown of UBA52 significantly decreases H5N1 viral titer and attenuates proinflammatory cytokine production, establishing UBA52 as a host factor required for influenza replication. Immunoprecipitation followed by mass spectrometry, UBA52 knockdown, viral titer measurement, cytokine production assay Frontiers in microbiology Medium 29867845
2002 The UbA52 promoter contains functional glucose-responsive E-box sequences and stress response elements (STRE); mutation of the first E-box or STRE reduces glucose-induced promoter activity; UbA52 mRNA and protein are upregulated in renal tubules of diabetic mice in proportion to blood glucose levels, and in MDCK cells under high-glucose conditions in both cell lysates and ribosomal fractions. Promoter deletion constructs with SEAP reporter assay, site-directed mutagenesis of E-box and STRE, in situ hybridization, immunohistochemistry, ribosomal fractionation The Journal of biological chemistry Medium 12171997
2025 ROCK2 stabilizes DRP1 protein by competing with UBA52 to bind DRP1, thereby preventing UBA52-mediated ubiquitination and proteasomal degradation of DRP1; this ROCK2/UBA52/DRP1 axis drives mitochondrial dynamics and pemigatinib resistance in cholangiocarcinoma. Co-immunoprecipitation (ROCK2–DRP1 and UBA52–DRP1), DRP1 ubiquitination assay, ROCK2 knockdown/overexpression, DRP1 overexpression rescue experiments, in vivo xenograft model Cell death & disease Medium 40615369
2025 NUDT7 interacts with UBA52 and targets it for proteasomal degradation, thereby reducing UBA52-mediated K11/K27/K48-linked polyubiquitination of the lipogenic transcription factor SREBF1, stabilizing SREBF1 and promoting lipid droplet formation to support PRRSV replication. Co-immunoprecipitation (NUDT7–UBA52), ubiquitination assay (K11/K27/K48 linkage-specific), NUDT7 overexpression/knockdown, SREBF1 stability assay, lipid droplet staining, viral replication assay International journal of biological sciences Medium 41608635
2026 UBA52 overexpression in neurons promotes ubiquitination and degradation of the pro-apoptotic protein Daxx (identified by IP-LC/MS and Co-IP), reducing ICH-induced apoptosis and mitochondrial dysfunction; Daxx overexpression abolishes the neuroprotective effect of UBA52, placing UBA52 upstream of Daxx in the apoptosis pathway. IP-LC/MS, co-immunoprecipitation, AAV9-UBA52 overexpression in ICH mouse model, TUNEL assay, caspase-3/9 levels, Mitotracker/JC-1 staining, ATP measurement, Daxx rescue experiment Molecular neurobiology Medium 41553582
2008 UBA52 protein is restrictedly localized to the surface of marginal cells of the stria vascularis in the cochlea of rats and mice, as determined by confocal immunocytochemistry and postembedding immunogold electron microscopy. Confocal microscopic immunocytochemistry on cryostat sections, postembedding immunogold cytochemistry (ultrastructural localization) Neuroscience Medium 18448257
2025 Sparc binds UBA52 (confirmed by immunoprecipitation/mass spectrometry and co-immunoprecipitation) and upregulates its expression in microglia; UBA52 knockdown abolishes the anti-inflammatory and mitochondrial-protective effects of Sparc overexpression in LPS-stimulated BV2 cells, and also reverses Sparc-mediated neuroprotection in co-cultured HT-22 neurons. Immunoprecipitation + mass spectrometry, co-immunoprecipitation, immunofluorescence, Uba52 siRNA epistasis, cytokine ELISA, mitochondrial membrane potential assay, neurite outgrowth assay Frontiers in bioscience (Landmark edition) Medium 40917064
2026 EMMPRIN downregulates UBA52 expression, reducing the free ubiquitin pool and decreasing K63-linked polyubiquitination of monocarboxylate transporter 1 (MCT1), destabilizing MCT1 and promoting global protein lactylation; this UBA52-MCT1 axis mediates EMMPRIN-driven metabolic reprogramming in metabolic-associated steatohepatitis. Proteomic sequencing, mass spectrometry, co-immunoprecipitation, Western blotting, EMMPRIN hepatocyte-specific KO and overexpression mouse models, K63 ubiquitination analysis Frontiers in pharmacology Medium 41660506
2025 During non-segmented negative-sense virus infection, rpL40 (the ribosomal protein product of UBA52 cleavage) is recruited to a noncanonical site on the small (40S) ribosomal subunit near the mRNA entry site, forming specialized ribosomes that preferentially bind and translate viral mRNAs; this remodeling pathway is co-opted from an endogenous metabolic stress response that alters ribosome composition to promote cell survival. Cryo-EM or structural ribosome analysis (implied by site identification), viral infection models, preferential mRNA binding assays, metabolic stress experiments bioRxivpreprint Medium bio_10.1101_2025.10.24.684008

Source papers

Stage 0 corpus · 30 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2016 The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development. Scientific reports 97 27829658
2019 LUCAT1 promotes colorectal cancer tumorigenesis by targeting the ribosomal protein L40-MDM2-p53 pathway through binding with UBA52. Cancer science 93 30690837
2019 Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. American journal of translational research 38 31814919
2024 SMYD5 methylation of rpL40 links ribosomal output to gastric cancer. Nature 30 39048817
2018 Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52) is essential for preimplantation embryo development. Biology open 29 30135083
2008 The localization of proteins encoded by CRYM, KIAA1199, UBA52, COL9A3, and COL9A1, genes highly expressed in the cochlea. Neuroscience 24 18448257
2022 UBA52 Is Crucial in HSP90 Ubiquitylation and Neurodegenerative Signaling during Early Phase of Parkinson's Disease. Cells 22 36497031
2002 Isolation and functional analysis of mouse UbA52 gene and its relevance to diabetic nephropathy. The Journal of biological chemistry 22 12171997
1994 Localization of the human UBA52 ubiquitin fusion gene to chromosome band 19p13.1-p12. Genomics 20 8188300
2018 Host Interaction Analysis of PA-N155 and PA-N182 in Chicken Cells Reveals an Essential Role of UBA52 for Replication of H5N1 Avian Influenza Virus. Frontiers in microbiology 17 29867845
2024 Inhibition of UBA52 induces autophagy via EMC6 to suppress hepatocellular carcinoma tumorigenesis and progression. Journal of cellular and molecular medicine 13 38445807
2024 SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma. Cell research 13 39103523
2023 UBA52 Attunes VDAC1-Mediated Mitochondrial Dysfunction and Dopaminergic Neuronal Death. ACS chemical neuroscience 13 36755387
2023 RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins, Are Not Essential for p53 Signalling. Biomolecules 9 37371478
1992 The human ubiquitin/52-residue ribosomal protein fusion gene subfamily (UbA52) is composed primarily of processed pseudogenes. Genomics 9 1330885
2023 Hydralazine represses Fpn ubiquitination to rescue injured neurons via competitive binding to UBA52. Journal of pharmaceutical analysis 7 38352945
2024 MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis. Autophagy 6 39193909
2023 UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair. The Journal of biological chemistry 6 37451480
2021 Clinical significance of UbA52 level in the urine of patients with type 2 diabetes mellitus and diabetic kidney disease. Nefrologia 5 36165137
2025 Targeting the ROCK2/UBA52/DRP1 axis enhances ferroptosis and overcomes pemigatinib resistance in Cholangiocarcinoma. Cell death & disease 4 40615369
2019 Toxoplasma gondii RPL40 is a circulating antigen with immune protection effect. Folia parasitologica 3 31592775
2014 A new reliable reference gene UBA52 for quantitative real-time polymerase chain reaction studies in pyloric cecal tissues of the starfish Asterias rubens. Genetics and molecular research : GMR 3 24938608
2025 UBA52 Mediates ribosomal DNA stability under hexavalent chromium exposure in occupational workers and cellular models. Ecotoxicology and environmental safety 1 40090168
2025 Sparc Suppresses Microglial Neuroinflammation and Promotes Axonal Regeneration by Interacting With Uba52. Frontiers in bioscience (Landmark edition) 1 40917064
2026 UBA52 Overexpression Ameliorates Intracerebral Hemorrhage-Associated Neuronal Apoptosis and Mitochondrial Dysfunction: A Protective Role in Neurons. Molecular neurobiology 0 41553582
2026 NUDT7 Modulates the UBA52-SREBF1 Signaling Axis to Promote PRRSV Replication via Lipid Synthesis. International journal of biological sciences 0 41608635
2026 EMMPRIN deficiency alleviated metabolic-associated steatohepatitis progression via regulation of the UBA52-MCT1 axis. Frontiers in pharmacology 0 41660506
2025 SMYD5 is a ribosomal methyltransferase that trimethylates RPL40 lysine 22 through recognition of a KXY motif. Cell reports 0 40184250
2025 Glutathion peroxidase 4 (GPX4) and Ribosomal Protein L40 (RPL40) participate in arsenic induced progression of renal cell carcinoma by regulating the NLRP3 mediated classic pyroptosis pathway. International journal of biological macromolecules 0 40239794
2025 Study on the molecular mechanism of UBA52 and BARD1 regulating hepatocellular carcinoma through the PI3 K/AKT signaling pathway. Discover oncology 0 40397202