Affinage

TAF15

TATA-binding protein-associated factor 2N · UniProt Q92804

Length
592 aa
Mass
61.8 kDa
Annotated
2026-04-28
100 papers in source corpus 25 papers cited in narrative 25 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TAF15 is a multifunctional FET-family RNA/ssDNA-binding protein that participates in transcription initiation, RNA processing, and post-transcriptional gene regulation. It co-purifies with a subpopulation of TFIID (via TBP) and RNA Polymerase II, entering the preinitiation complex, and its low-complexity domain forms amyloid-like fibrils that bind the Pol II CTD (PMID:8890175, PMID:28945358). TAF15 binds ~4,900 neuronal RNAs enriched for GGUA motifs via a structurally non-canonical RRM domain, regulating alternative splicing and RNA turnover, and forms a novel chromatin-associated U1-TAF15 snRNP distinct from spliceosomal U1 snRNP (PMID:27378374, PMID:26612539, PMID:19282884). Its activity is controlled by arginine methylation (PRMT1), PKA phosphorylation that alters RNA-binding specificity, multi-mono-ubiquitylation by TIF1γ driving nuclear export, and Parkin-mediated ubiquitin-dependent degradation; cryo-EM of FTLD-FUS patient brains revealed that the disease-defining amyloid filaments are composed of TAF15 residues 7–99, not FUS (PMID:19124016, PMID:38568213, PMID:36261009, PMID:30339961, PMID:38057661).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1996 High

    Establishing TAF15 as a novel TBP-associated factor resolved the question of whether RNA-binding proteins participate directly in basal transcription complexes, placing TAF15 at the intersection of transcription initiation and RNA metabolism.

    Evidence Biochemical co-purification of hTAFII68 with TFIID and Pol II, RNA/ssDNA binding assays

    PMID:8890175

    Open questions at the time
    • No determination of whether TAF15 is catalytically active within TFIID
    • Structural basis of TAF15–TBP interaction unknown at this stage
  2. 1998 High

    Demonstrating that TAF15 and EWS bind the same TFIID subunits in a mutually exclusive manner established that FET proteins occupy overlapping but distinct niches in the transcription machinery.

    Evidence In vitro binding studies with recombinant TFIID and Pol II subunits

    PMID:9488465

    Open questions at the time
    • Physiological stoichiometry of TAF15- vs. EWS-containing TFIID not determined
    • No genome-wide identification of genes specifically dependent on TAF15-containing TFIID
  3. 2004 Medium

    Identification of Src-mediated tyrosine phosphorylation of TAF15, which stimulates its transactivation function, revealed that signal transduction pathways directly modulate TAF15 transcriptional activity.

    Evidence In vitro/in vivo kinase assays, co-IP with SH3 domains, reporter assays

    PMID:15094065

    Open questions at the time
    • Specific phosphorylated residues not mapped
    • Physiological relevance of v-Src versus c-Src in normal cells unclear
  4. 2008 High

    Discovery that PRMT1 methylates TAF15 RGG repeats and that this modification controls subcellular localization and target gene expression established arginine methylation as a key regulatory switch for TAF15 function.

    Evidence In vivo methylation, co-IP with PRMT1, subcellular fractionation, gene expression profiling

    PMID:19124016

    Open questions at the time
    • Individual methylated arginine sites not fully mapped
    • Downstream effectors reading TAF15 methylation marks unidentified
  5. 2008 Medium

    Localization of TAF15 to stress granules upon heat shock and oxidative stress, and to spreading initiation centers, revealed cytoplasmic roles beyond nuclear transcription.

    Evidence Immunofluorescence, live cell imaging under stress conditions

    PMID:18620564

    Open questions at the time
    • Domain requirements for TAF15 stress-granule targeting not mapped (only FUS was dissected)
    • Functional consequence of TAF15 in stress granules not determined
  6. 2009 High

    Identification of a novel chromatin-associated U1-TAF15 snRNP, distinct from canonical spliceosomal U1 snRNP, opened the question of non-canonical snRNP functions and showed TAF15 can reorganize snRNA into alternative complexes.

    Evidence Reciprocal co-IPs, RNA co-precipitation, fluorescence microscopy, transcriptional inhibition

    PMID:19282884

    Open questions at the time
    • Function of U1-TAF15 snRNP on chromatin unknown
    • Whether U1-TAF15 particle has any role in splicing versus transcription unresolved
  7. 2009 Medium

    Mapping caspase-3/7 cleavage of TAF15 at 106DQPD/Y110 showed that TAF15 is proteolytically processed during apoptosis, potentially separating its LC domain from RNA-binding functions.

    Evidence In vitro caspase cleavage, site-directed mutagenesis, cell-based apoptosis

    PMID:19426707

    Open questions at the time
    • Biological consequence of caspase cleavage fragments not characterized
    • Whether cleavage contributes to disease-associated aggregation unknown
  8. 2011 Medium

    Demonstrating Transportin-dependent nuclear import of TAF15 and its accumulation in cytoplasmic inclusions when import is blocked linked FET protein mislocalization to neurodegenerative disease mechanisms, and TAF15 was found in FTLD but not ALS-FUS inclusions.

    Evidence Transportin inhibition, immunohistochemistry of FTLD/ALS tissue, immunoblot of insoluble fractions

    PMID:21856723 PMID:22771914

    Open questions at the time
    • Whether TAF15 NLS mutations are causative in human FTLD not established
    • Mechanism converting cytoplasmic mislocalization to toxicity unknown
  9. 2012 Medium

    Loss-of-function experiments revealed that TAF15 promotes cell proliferation and suppresses apoptosis through a miRNA-mediated pathway: TAF15 sustains miR-17/20a levels, thereby repressing CDKN1A/p21.

    Evidence siRNA knockdown, gene expression profiling, miRNA quantification, flow cytometry

    PMID:23128393

    Open questions at the time
    • Whether TAF15 directly binds or stabilizes pri-miR-17 transcript not shown
    • Generalizability beyond the cell lines tested not established
  10. 2013 High

    Genome-wide CLIP-seq in brain tissue identified conserved TAF15 RNA targets and demonstrated regulation of Grin1 splicing, directly linking TAF15 to neuronal RNA processing and NMDA receptor function.

    Evidence iCLIP, RNA-seq with TAF15 knockdown in mouse neurons and human brain

    PMID:23416048

    Open questions at the time
    • Functional consequence of Grin1 mis-splicing on neuronal physiology not tested
    • Redundancy between TAF15 and FUS in splicing regulation not fully resolved
  11. 2013 High

    Identification of the FETBM1 motif mediating RNA/DNA-independent homo- and hetero-dimerization among FET proteins established a structural basis for FET protein complex formation and explained how oncogenic fusion proteins sequester wild-type FET proteins.

    Evidence Recombinant protein pulldowns, mass spectrometry, motif mutagenesis

    PMID:23975937

    Open questions at the time
    • Stoichiometry and structure of FET complexes in vivo unknown
    • Whether FETBM1-mediated dimerization is regulated by post-translational modifications not tested
  12. 2015 High

    NMR structure of the TAF15 RRM revealed a non-canonical RNA recognition mode using hydrogen bonding on a concave surface rather than classical aromatic stacking, explaining structure-dependent rather than sequence-dependent RNA binding.

    Evidence Solution NMR, calorimetry, molecular dynamics, RNA variant binding assays

    PMID:26612539

    Open questions at the time
    • No full-length TAF15–RNA structure available
    • How RRM and RGG domains cooperate in target recognition not resolved
  13. 2016 High

    Comprehensive CLIP-seq and RNA Bind-N-Seq refined the TAF15 binding landscape to ~4,900 RNAs with GGUA motif enrichment and revealed that, unlike FUS, TAF15 primarily affects RNA turnover rather than alternative splicing in neural progenitors.

    Evidence CLIP-seq, RNA Bind-N-Seq, RNA-seq in mouse brain and human neural progenitors

    PMID:27378374

    Open questions at the time
    • Mechanism by which TAF15 controls RNA stability (direct degradation vs. indirect) unknown
    • Discrepancy with earlier splicing findings in neurons not fully reconciled
  14. 2017 High

    Demonstration that TAF15 LC domain fibrils bind Pol II CTD heptads, with lysine-7 contributing electrostatic contacts, provided a molecular mechanism linking TAF15 phase behavior to transcription machinery recruitment.

    Evidence NMR, hydrogel FRAP, CTD mutagenesis

    PMID:28945358

    Open questions at the time
    • Whether fibril–CTD interaction occurs in living cells not confirmed
    • Relationship between pathological amyloid filaments and functional LC fibrils unclear
  15. 2018 Medium

    Parkin was identified as a second E3 ubiquitin ligase for TAF15, with parkin overexpression reducing TAF15 levels and suppressing TAF15-induced neurotoxicity in Drosophila, linking TAF15 turnover to Parkinson's-relevant quality control pathways.

    Evidence Co-IP, E3 activity assays, Drosophila genetic rescue

    PMID:30339961

    Open questions at the time
    • Specific ubiquitylation sites on TAF15 by Parkin not mapped
    • Relevance to mammalian neurodegeneration not validated
  16. 2022 High

    TIF1γ was shown to multi-mono-ubiquitylate TAF15, driving its nuclear export and competing with TAF15 for TBP binding, thereby suppressing TAF15-dependent transcription (e.g., IL-6) and EMT/metastasis in lung adenocarcinoma.

    Evidence Competition assays, ubiquitylation assays, nuclear export assays, luciferase reporters, cell migration/invasion

    PMID:36261009

    Open questions at the time
    • Ubiquitylated residues on TAF15 not mapped
    • Whether TIF1γ and Parkin target overlapping or distinct TAF15 pools unclear
  17. 2022 Medium

    TAF15 RGG domain was found to inhibit SRPK1 kinase activity, causing SR protein hypophosphorylation and splicing inhibition, revealing a non-RNA-binding mechanism by which TAF15 regulates splicing.

    Evidence Co-IP, kinase activity assays, reporter minigene splicing, immunofluorescence

    PMID:36611919

    Open questions at the time
    • Whether endogenous TAF15 levels are sufficient to modulate SRPK1 in vivo not shown
    • Genome-wide splicing effects of TAF15-SRPK1 axis not profiled
  18. 2023 High

    Cryo-EM of FTLD-FUS patient brain filaments revealed they are composed of TAF15 (residues 7–99), not FUS, redefining the molecular identity of FTLD-FUS as a TAF15 amyloidosis and providing a structural template for the disease.

    Evidence Cryo-EM structure determination from four independent FTLD-FUS patient brains

    PMID:38057661

    Open questions at the time
    • Whether TAF15 filament formation is a cause or consequence of neurodegeneration unknown
    • Conditions that seed TAF15 amyloid formation in vivo not identified
  19. 2024 Medium

    PKA was identified as a nuclear kinase that phosphorylates TAF15 and alters its RNA-binding profile, shifting target specificity toward transcripts involved in mRNA maturation and splicing.

    Evidence iCLIP comparing phosphorylated vs. unphosphorylated TAF15 upon cAMP-PKA activation

    PMID:38568213

    Open questions at the time
    • Specific phosphorylated residues not identified
    • How PKA phosphorylation interacts with PRMT1 methylation or TIF1γ ubiquitylation unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major unresolved questions include the precise cellular function of the U1-TAF15 snRNP, the relationship between physiological LC-domain phase separation and pathological amyloid formation, and how multiple post-translational modifications (methylation, phosphorylation, ubiquitylation) are integrated to control TAF15 activity in different cell types.
  • No reconstitution of U1-TAF15 snRNP function
  • No structure of full-length TAF15 or its complexes with TFIID
  • No systematic dissection of PTM crosstalk on TAF15

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 5 GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005634 nucleus 5 GO:0005829 cytosol 3 GO:0005694 chromosome 1 GO:0005730 nucleolus 1
Pathway
R-HSA-74160 Gene expression (Transcription) 5 R-HSA-392499 Metabolism of proteins 4 R-HSA-8953854 Metabolism of RNA 3 R-HSA-1643685 Disease 1
Partners
HNRNPMPRKNPRMT1RELASNRPCSRPK1TBPTIF1Γ
Complex memberships
FET homo/heterodimersTFIID (subpopulation)U1-TAF15 snRNP

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 hTAF15 (hTAFII68) was identified as a novel TBP-associated factor (TAF) that co-purifies with a subpopulation of TFIID complexes and with RNA polymerase II, and can enter the preinitiation complex together with Pol II. It contains a consensus RNA-binding domain (RNP-CS) and binds both RNA and single-stranded DNA. Biochemical co-purification, cloning and characterization, RNA/ssDNA binding assays The EMBO journal High 8890175
1998 In vitro binding studies revealed that hTAFII68 (TAF15) interacts with specific TFIID subunits and with RNA Polymerase II subunits, and that EWS and hTAFII68 interact with the same TFIID subunits, suggesting their presence in TFIID is mutually exclusive. In vitro binding studies, co-immunoprecipitation, biochemical fractionation Molecular and cellular biology High 9488465
2008 Endogenous TAF15 is methylated in vivo at its RGG repeats by PRMT1 (identified as a TAF15 interactor and the major PRMT responsible). Methylation of RGG repeats affects subcellular localization and is required for TAF15 to positively regulate expression of its target genes. In vivo methylation assays, co-immunoprecipitation, subcellular fractionation, gene expression assays, loss-of-function Experimental cell research High 19124016
2009 A fraction of human U1 snRNA specifically associates with TAF15 to form a novel chromatin-associated U1-TAF15 snRNP that is distinct from the spliceosomal U1-Sm snRNP; none of the known U1-Sm snRNP protein components interact with the U1-TAF15 particle. This particle accumulates in nucleolar caps upon transcriptional inhibition and its biogenesis depends on the Sm-binding motif of U1 snRNA. Immunoprecipitation, RNA co-precipitation, fluorescence microscopy, transcriptional inhibition experiments EMBO reports High 19282884
2011 TAF15 associates with a subset of the spliceosomal U1 snRNP complex via direct protein-protein interaction between the N-terminal domain of TAF15 and U1C protein, as demonstrated by pulldown assays with recombinant proteins and immunoprecipitation of U1 snRNA and Sm proteins with TAF15 antibodies. Immunoprecipitation, pulldown with recombinant proteins, UV cross-linking, co-precipitation Biochimica et biophysica acta Medium 22019700
2011 All endogenous FET proteins (including TAF15) are recruited into cytoplasmic stress granules upon general inhibition of Transportin-mediated nuclear import, implicating Transportin-dependent nuclear import in maintaining TAF15 nuclear localization. TAF15 co-accumulates with FUS in FTLD inclusions but not in ALS-FUS inclusions. Cell culture experiments, Transportin inhibition, immunohistochemistry, immunoblot of insoluble fractions Brain : a journal of neurology Medium 21856723
2012 The C-terminus of TAF15 contains a Transportin-dependent nuclear localization signal (NLS), and its RGG domain can be targeted to stress granules. TAF15 cellular localization depends on ongoing transcription, and TAF15 co-localizes with RNA granules in the cytoplasm of neuronal HT22 cells in a cell-type-dependent manner. Domain deletion and mutagenesis, live imaging, immunofluorescence, transcriptional inhibition Gene Medium 22771914
2012 TAF15 knockdown reduces cellular proliferation and increases apoptosis. TAF15 regulates expression of cell cycle genes post-transcriptionally through a miRNA pathway: TAF15 depletion decreases levels of onco-miR-17 locus miRNAs (miR-17-5p and miR-20a), leading to upregulation of CDKN1A/p21. siRNA knockdown, global gene expression profiling, miRNA analysis, flow cytometry Oncogene Medium 23128393
2013 FUS, EWSR1, and TAF15 form homo- and heterocomplexes via a conserved N-terminal motif (FETBM1). This interaction is RNA- and DNA-independent and robust under high-salt conditions. The FETBM1 motif is required for complex formation and also for binding of normal full-length FET proteins to their oncogenic fusion proteins. Pulldown with recombinant proteins, mass spectrometry, mutagenesis FASEB journal High 23975937
2013 CLIP-seq and RNA-seq in human brain and mouse neurons identified conserved TAF15 RNA binding targets; TAF15 regulates alternative splicing of neuronal RNAs, including a critical splicing event in the Grin1 (NMDA receptor zeta-1 subunit) transcript that controls NR1 activity and trafficking. CLIP-seq (iCLIP), RNA-seq, TAF15 knockdown Cell reports High 23416048
2014 TAF15 co-immunoprecipitates preferentially with hnRNP M3/4 isoforms (higher MW), while TLS/FUS associates with hnRNP M1/2 (lower MW), via direct protein-protein interactions through the amino-termini of the TET proteins, independently of RNA. Immunoprecipitation, pulldown with recombinant proteins, co-localization Molecular biology reports Medium 24474660
2015 NMR structure of the TAF15 RRM domain reveals a non-canonical mode of RNA recognition: binding to stem-loop RNA is mediated primarily by hydrogen bonding between RNA bases and a concave face on the RRM surface rather than classical stacking interactions at RNP sites. RNA binding is dependent on structural elements in the RNA rather than sequence alone. Solution NMR spectroscopy, calorimetry, docking, molecular dynamics simulation Scientific reports High 26612539
2016 CLIP-seq and RNA Bind-N-Seq in mouse brains showed TAF15 binds ~4,900 RNAs enriched for GGUA motifs. TAF15 and FUS show similar binding in introns and 3'UTRs, but unlike FUS and TDP-43, TAF15 has a minimal role in alternative splicing. In human neural progenitors, TAF15 and FUS affect RNA target turnover. CLIP-seq, RNA Bind-N-Seq, RNA-seq, loss-of-function in multiple cell types Nature communications High 27378374
2017 TAF15 low-complexity (LC) domain forms amyloid-like fibrils that bind RNA Pol II CTD. NMR and fluorescence microscopy (FRAP) showed the interaction involves heptads throughout CTD, with lysines at position 7 contributing through electrostatic interactions; mutation of these lysines to consensus serines reduced binding. NMR spectroscopy, hydrogel fluorescence microscopy, FRAP, mutagenesis Biochemistry High 28945358
2017 PRMT1 shows differential interaction with RGG-boxes of TAF15 compared to FUS and EWS. The Asp residue in TAF15's YGGDR(S/G)G repeats confers poor binding to PRMT1, suggesting reduced overall methylation of TAF15 compared to other FET proteins and contributing to TAF15 functional specialization. Peptide-based binding assays, novel 2-hybrid binding assay, mutagenesis Protein science Medium 29193371
2004 TAF15 (hTAFII68) is phosphorylated on tyrosine residue(s) by v-Src kinase in vitro and in vivo, and TAF15 associates with SH3 domains of v-Src and other cell signaling proteins. v-Src stimulates TAF15-mediated transcriptional activation, while dominant-negative Src reduces TAF15 transactivation function. In vitro kinase assays, in vivo tyrosine phosphorylation, co-immunoprecipitation with SH3 domains, transactivation reporter assays FEBS letters Medium 15094065
2009 TAF15 and the leukemia-associated fusion protein TAF15-CIZ/NMP4 are specifically cleaved by caspases-3 and -7 at the consensus sequence 106DQPD/Y110 as identified by mutagenesis. In vitro caspase cleavage assays, site-directed mutagenesis, cell-based apoptosis assays Biochemical and biophysical research communications Medium 19426707
2022 TAF15 C-terminal RGG domain associates with SRPK1 and inhibits its kinase activity, causing partial relocalization of SRPK1 to the nucleus, hypophosphorylation of SR proteins, inhibition of splicing of a reporter minigene, and inhibition of Lamin B receptor phosphorylation. Co-immunoprecipitation, kinase activity assays, overexpression, reporter minigene splicing assay, immunofluorescence Cells Medium 36611919
2022 TIF1γ binds TBP in competition with TAF15 and impedes TAF15/TBP-mediated IL-6 transactivation. TIF1γ modifies TAF15 through multi-mono-ubiquitylation and drives nuclear export of TAF15, thereby inhibiting TAF15-promoted EMT and metastasis in lung adenocarcinoma cells. Co-immunoprecipitation, competition assays, luciferase reporter, ubiquitylation assays, immunofluorescence, nuclear export assays Cell reports High 36261009
2023 Cryo-EM structures of amyloid filaments extracted from FTLD-FUS patient brains show that the filaments are composed of TAF15, not FUS. The filament fold is formed from residues 7-99 of the low-complexity domain (LCD) of TAF15 and was identical across four individuals. Cryo-EM structure determination of patient-extracted amyloid filaments Nature High 38057661
2023 TAF15 bound directly to the FASN promoter to facilitate FASN expression (promoting hepatic steatosis), and interacted with p65 NF-κB to activate NF-κB signaling and increase proinflammatory cytokine secretion in NASH. ChIP-seq (CUT&Tag), dual-luciferase reporter, co-immunoprecipitation, immunofluorescence, AAV-mediated knockdown/overexpression in mice Liver international Medium 37183512
2024 TAF15 is a nuclear PKA substrate; TAF15 phosphorylation by PKA alters its binding to target transcripts involved in mRNA maturation, splicing, and protein-binding functions, as demonstrated by iCLIP experiments comparing phosphorylated and unphosphorylated states. iCLIP (cross-linking immunoprecipitation), cAMP-PKA pathway activation, phosphorylation assays Cellular and molecular life sciences Medium 38568213
2018 Parkin (E3 ubiquitin ligase) directly binds TAF15, and parkin overexpression reduces TAF15 protein levels through its E3 ubiquitin ligase activity. In a Drosophila model, parkin overexpression suppresses TAF15-induced neurotoxicity (lifespan defects, locomotive activity defects). Co-immunoprecipitation, in vivo Drosophila model, genetic rescue, ubiquitin ligase activity assays Neurobiology of aging Medium 30339961
2021 Taf15 regulates dorsoanterior neural development in Xenopus through at least two mechanisms: (1) retention of a single fgfr4 intron (post-transcriptional/splicing regulation) when maternal+zygotic Taf15 is depleted, and (2) reduction in total fgfr4 transcript (transcriptional regulation) when only zygotic Taf15 is depleted. Morpholino-mediated depletion, RNA-seq (exon usage and transcript abundance), Xenopus loss-of-function Development (Cambridge, England) Medium 34345915
2008 FUS and TAF15 proteins were detected in spreading initiation centers of adhering cells and are targeted to stress granules induced by heat shock and oxidative stress. FUS requires its RNA-binding domain for translocation to stress granules. Immunofluorescence, live cell imaging, stress granule induction assays, domain deletion analysis BMC cell biology Medium 18620564

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II. The EMBO journal 299 8890175
2008 The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC cell biology 274 18620564
2011 FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations. Brain : a journal of neurology 244 21856723
1998 EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes. Molecular and cellular biology 224 9488465
2011 Mutational analysis reveals the FUS homolog TAF15 as a candidate gene for familial amyotrophic lateral sclerosis. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 145 21438137
2016 Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses. Nature communications 133 27378374
1999 Fusion of the EWS-related gene TAF2N to TEC in extraskeletal myxoid chondrosarcoma. Cancer research 114 10537274
2010 Dr. Jekyll and Mr. Hyde: The Two Faces of the FUS/EWS/TAF15 Protein Family. Sarcoma 112 21197473
2002 Recurrent rearrangement of the Ewing's sarcoma gene, EWSR1, or its homologue, TAF15, with the transcription factor CIZ/NMP4 in acute leukemia. Cancer research 99 12359745
2005 Arginine methylation of yeast mRNA-binding protein Npl3 directly affects its function, nuclear export, and intranuclear protein interactions. The Journal of biological chemistry 92 15998636
1996 Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3. Genetics 87 8770588
1998 Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes. Gene 85 9795213
2008 A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast. Molecular cell 84 19061647
1999 Identification of a novel fusion gene involving hTAFII68 and CHN from a t(9;17)(q22;q11.2) translocation in an extraskeletal myxoid chondrosarcoma. Oncogene 84 10602520
2008 PRMT1 mediated methylation of TAF15 is required for its positive gene regulatory function. Experimental cell research 78 19124016
2005 Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II. The EMBO journal 75 15902270
2013 The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability. Genes & development 67 24240235
2008 Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PloS one 66 18818768
1999 Fusion of the RBP56 and CHN genes in extraskeletal myxoid chondrosarcomas with translocation t(9;17)(q22;q11). Oncogene 64 10602519
1995 Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae. Genetics 59 8582634
2020 lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation. Molecular therapy. Nucleic acids 56 32464546
2009 Human U1 snRNA forms a new chromatin-associated snRNP with TAF15. EMBO reports 55 19282884
1996 Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins. Genomics 55 8954779
2012 TAF15 is important for cellular proliferation and regulates the expression of a subset of cell cycle genes through miRNAs. Oncogene 52 23128393
2012 The yeast SR-like protein Npl3 links chromatin modification to mRNA processing. PLoS genetics 47 23209445
2024 Insights into Molecular Diversity within the FUS/EWS/TAF15 Protein Family: Unraveling Phase Separation of the N-Terminal Low-Complexity Domain from RNA-Binding Protein EWS. Journal of the American Chemical Society 46 38492239
2007 Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI. RNA (New York, N.Y.) 44 17684230
2019 TRPM2-AS promotes cancer cell proliferation through control of TAF15. The international journal of biochemistry & cell biology 43 31887411
2020 LncRNA PITPNA-AS1 boosts the proliferation and migration of lung squamous cell carcinoma cells by recruiting TAF15 to stabilize HMGB3 mRNA. Cancer medicine 41 32871048
2019 USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1. Cell death & disease 41 30931936
2023 TAF15 amyloid filaments in frontotemporal lobar degeneration. Nature 40 38057661
2021 LncRNA GAS5 activates the HIF1A/VEGF pathway by binding to TAF15 to promote wound healing in diabetic foot ulcers. Laboratory investigation; a journal of technical methods and pathology 40 33875793
2013 A conserved N-terminal motif is required for complex formation between FUS, EWSR1, TAF15 and their oncogenic fusion proteins. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38 23975937
2012 Domains involved in TAF15 subcellular localisation: dependence on cell type and ongoing transcription. Gene 37 22771914
2013 Identification of in vivo, conserved, TAF15 RNA binding sites reveals the impact of TAF15 on the neuronal transcriptome. Cell reports 36 23416048
2017 Lysines in the RNA Polymerase II C-Terminal Domain Contribute to TAF15 Fibril Recruitment. Biochemistry 34 28945358
2011 The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits. EMBO reports 31 21852791
2019 NR4A3 fusion proteins trigger an axon guidance switch that marks the difference between EWSR1 and TAF15 translocated extraskeletal myxoid chondrosarcomas. The Journal of pathology 28 31020999
2011 Identification of the TAF15-ZNF384 fusion gene in two new cases of acute lymphoblastic leukemia with a t(12;17)(p13;q12). Cancer genetics 28 21504714
2016 α-Amanitin Restrains Cancer Relapse from Drug-Tolerant Cell Subpopulations via TAF15. Scientific reports 25 27181033
2011 mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Genes, chromosomes & cancer 25 21344536
2011 A fraction of the transcription factor TAF15 participates in interactions with a subset of the spliceosomal U1 snRNP complex. Biochimica et biophysica acta 24 22019700
2007 Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals. Journal of molecular biology 24 18022637
2023 CircDNAJC11 interacts with TAF15 to promote breast cancer progression via enhancing MAPK6 expression and activating the MAPK signaling pathway. Journal of translational medicine 23 36895010
2022 TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex. Cell reports 23 36261009
2020 Npl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence. EMBO reports 22 32026548
2013 Monosome formation during translation initiation requires the serine/arginine-rich protein Npl3. Molecular and cellular biology 22 24100011
2021 LncRNA APOA1-AS facilitates proliferation and migration and represses apoptosis of VSMCs through TAF15-mediated SMAD3 mRNA stabilization. Cell cycle (Georgetown, Tex.) 21 34382908
2009 The shuttling protein Npl3 promotes translation termination accuracy in Saccharomyces cerevisiae. Journal of molecular biology 21 19733178
2023 m6A-enriched lncRNA LINC00839 promotes tumor progression by enhancing TAF15-mediated transcription of amine oxidase AOC1 in nasopharyngeal carcinoma. The Journal of biological chemistry 20 37257820
2023 TAF15 exacerbates nonalcoholic steatohepatitis progression by regulating lipid metabolism and inflammation via FASN and p65 NF-κB. Liver international : official journal of the International Association for the Study of the Liver 18 37183512
2022 LncRNA LINC00649 recruits TAF15 and enhances MAPK6 expression to promote the development of lung squamous cell carcinoma via activating MAPK signaling pathway. Cancer gene therapy 18 35228660
2008 Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation. Molecular and cellular biology 18 18391019
2023 Epigallocatechin gallate prevents cardiomyocytes from pyroptosis through lncRNA MEG3/TAF15/AIM2 axis in myocardial infarction. Chinese medicine 17 38057891
2004 Stimulation of hTAFII68 (NTD)-mediated transactivation by v-Src. FEBS letters 17 15094065
2022 Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring SNHG7 to promote osteogenesis via TAF15-RUNX2 pathway. Regenerative medicine 16 36184881
2020 TAF15 contributes to the radiation-inducible stress response in cancer. Oncotarget 16 32676166
2010 Diagnostic and therapeutic potential of a human antibody cloned from a cancer patient that binds to a tumor-specific variant of transcription factor TAF15. Cancer research 16 20048082
2022 Long non-coding RNA SNHG4 enhances RNF14 mRNA stability to promote the progression of colorectal cancer by recruiting TAF15 protein. Apoptosis : an international journal on programmed cell death 15 36482019
2021 STAT1 mediated long non-coding RNA LINC00504 influences radio-sensitivity of breast cancer via binding to TAF15 and stabilizing CPEB2 expression. Cancer biology & therapy 15 34908514
2020 miR-182-5p inhibits the pathogenic Th17 response in experimental autoimmune uveitis mice via suppressing TAF15. Biochemical and biophysical research communications 15 32736708
2004 Chemical clamping allows for efficient phosphorylation of the RNA carrier protein Npl3. The Journal of biological chemistry 13 15145958
2023 Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic acids research 12 36583366
2023 TAF15 regulates the BRD4/GREM1 axis and activates the gremlin-1-NF-κB pathway to promote OA progression. Regenerative therapy 12 37496731
2022 LncRNA HOTTIP facilitates osteogenic differentiation in bone marrow mesenchymal stem cells and induces angiogenesis via interacting with TAF15 to stabilize DLX2. Experimental cell research 12 35644412
2022 LncRNA MIR9-3HG enhances LIMK1 mRNA and protein levels to contribute to the carcinogenesis of lung squamous cell carcinoma via sponging miR-138-5p and recruiting TAF15. Pathology, research and practice 12 35933883
2021 Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling. Nature communications 12 34035302
2018 Characterization of the Interaction between Arginine Methyltransferase Hmt1 and Its Substrate Npl3: Use of Multiple Cross-Linkers, Mass Spectrometric Approaches, and Software Platforms. Analytical chemistry 12 30004689
2021 lncRNA PAPPA-AS1 Induces the Development of Hypertrophic Scar by Upregulating TLR4 through Interacting with TAF15. Mediators of inflammation 11 34285657
2015 Structural delineation of stem-loop RNA binding by human TAF15 protein. Scientific reports 11 26612539
2023 TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway. Scientific reports 10 37037864
2023 TAF15::NR4A3 gene fusion identifies a morphologically distinct subset of extraskeletal myxoid chondrosarcoma mimicking myoepithelial tumors. Genes, chromosomes & cancer 10 37057757
2023 lncRNA GMDS-AS1 restrains lung adenocarcinoma progression via recruiting TAF15 protein to stabilize SIRT1 mRNA. Epigenomics 10 37309595
2022 Octreotide ameliorates hepatic ischemia-reperfusion injury through SNHG12/TAF15-mediated Sirt1 stabilization and YAP1 transcription. Toxicology and applied pharmacology 10 35307376
2017 Differential interaction of PRMT1 with RGG-boxes of the FET family proteins EWS and TAF15. Protein science : a publication of the Protein Society 10 29193371
2014 Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS. Molecular biology reports 10 24474660
2011 Characterization and expression analysis in the developing embryonic brain of the porcine FET family: FUS, EWS, and TAF15. Gene 10 22143032
2022 YY1-induced lncRNA XIST inhibits cartilage differentiation of BMSCs by binding with TAF15 to stabilizing FUT1 expression. Regenerative therapy 9 35402663
2016 Mixed Phenotype Acute Leukemia with t(12;17)(p13;q21)/TAF15-ZNF384 and Other Chromosome Abnormalities. Cytogenetic and genome research 9 27607436
2023 Extraskeletal Myxoid Chondrosarcomas: The Uncommon Clinicopathologic Manifestations and Significance of TAF15::NR4A3 Fusion. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 8 36948401
2018 An Adult Patient with Early Pre-B Acute Lymphoblastic Leukemia with t(12;17)(p13;q21)/ZNF384-TAF15. In vivo (Athens, Greece) 8 30150451
2014 Npl3, a new link between RNA-binding proteins and the maintenance of genome integrity. Cell cycle (Georgetown, Tex.) 8 24694687
2023 NDRG1 promotes endothelial dysfunction and hypoxia-induced pulmonary hypertension by targeting TAF15. Precision clinical medicine 7 37885911
2022 Identification of a novel translocation producing an in-frame fusion of TAF15 and ETV4 in a case of extraosseous Ewing sarcoma revealed in the prenatal period. Virchows Archiv : an international journal of pathology 7 35527322
2021 The SR-protein Npl3 is an essential component of the meiotic splicing regulatory network in Saccharomyces cerevisiae. Nucleic acids research 7 33577675
2017 The RNA binding protein Npl3 promotes resection of DNA double-strand breaks by regulating the levels of Exo1. Nucleic acids research 7 28472517
2024 CypA/TAF15/STAT5A/miR-514a-3p feedback loop drives ovarian cancer metastasis. Oncogene 6 39402372
2017 Protein arginine methylation of Npl3 promotes splicing of the SUS1 intron harboring non-consensus 5' splice site and branch site. Biochimica et biophysica acta. Gene regulatory mechanisms 6 28392442
2021 The atypical RNA-binding protein Taf15 regulates dorsoanterior neural development through diverse mechanisms in Xenopus tropicalis. Development (Cambridge, England) 5 34345915
2024 TAF15 inhibits p53 nucleus translocation and promotes HCC cell 5-FU resistance via post-transcriptional regulation of UBE2N. Journal of physiology and biochemistry 4 39446246
2022 The SGYS motif of TAF15 prion-like domain is critical to amyloid fibril formation. Biophysical journal 4 35643629
2022 circVMA21 combining with TAF15 stabilizes SOCS3 mRNA to relieve septic lung injury through regulating NF-κB activation. Molecular immunology 4 36162226
2018 Genetic activation of parkin rescues TAF15-induced neurotoxicity in a Drosophila model of amyotrophic lateral sclerosis. Neurobiology of aging 4 30339961
2025 Targeting PRMT1-mediated methylation of TAF15 to protect against myocardial infarction by inhibiting ferroptosis via the GPX4/NRF2 pathway. Clinical epigenetics 3 40696470
2024 Promotive actions of lncRNA EBLN3P involved in cervical cancer progression via interacting with miR-29c-3p and TAF15 to modify RCC2. Archives of biochemistry and biophysics 3 38555043
2024 Phosphorylation of the compartmentalized PKA substrate TAF15 regulates RNA-protein interactions. Cellular and molecular life sciences : CMLS 3 38568213
2023 The Saccharomyces cerevisiae SR protein Npl3 interacts with hyperphosphorylated CTD of RNA Polymerase II. International journal of biological macromolecules 3 37858651
2022 Reduced oxidative stress suppresses neurotoxicity in the Drosophila model of TAF15-associated proteinopathies. Molecular brain 3 36411469
2009 TAF15 and the leukemia-associated fusion protein TAF15-CIZ/NMP4 are cleaved by caspases-3 and -7. Biochemical and biophysical research communications 3 19426707
2022 SR Protein Kinase 1 Inhibition by TAF15. Cells 2 36611919