Affinage

RELA

Transcription factor p65 · UniProt Q04206

Audit flag: rerun needed
Length
551 aa
Mass
60.2 kDa
Annotated
2026-04-28
130 papers in source corpus

Mechanistic narrative

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Mechanism profile

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Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 The NF-kappa B and I kappa B proteins: new discoveries and insights. Annual review of immunology 5257 8717528
2012 Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease. Nature 3725 23128233
1986 Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell 2401 3091258
2004 Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase. The EMBO journal 2318 15152190
1988 I kappa B: a specific inhibitor of the NF-kappa B transcription factor. Science (New York, N.Y.) 1972 3140380
2003 Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. The Journal of biological chemistry 1852 12527755
1997 A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1833 9252186
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2002 Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Molecular cell 1266 11983170
2009 A census of human transcription factors: function, expression and evolution. Nature reviews. Genetics 1191 19274049
2008 Identification of host proteins required for HIV infection through a functional genomic screen. Science (New York, N.Y.) 1165 18187620
1995 Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway. Genes & development 1164 7628694
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2001 Duration of nuclear NF-kappaB action regulated by reversible acetylation. Science (New York, N.Y.) 1046 11533489
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
1998 Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Molecular cell 1009 9660950
2005 Toll-like receptor-mediated cytokine production is differentially regulated by glycogen synthase kinase 3. Nature immunology 938 16007092
2004 Oscillations in NF-kappaB signaling control the dynamics of gene expression. Science (New York, N.Y.) 933 15499023
1999 Peroxisome proliferator-activated receptor alpha negatively regulates the vascular inflammatory gene response by negative cross-talk with transcription factors NF-kappaB and AP-1. The Journal of biological chemistry 931 10542237
2009 SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span. Cell 917 19135889
1994 Physical association and functional antagonism between the p65 subunit of transcription factor NF-kappa B and the glucocorticoid receptor. Proceedings of the National Academy of Sciences of the United States of America 898 8290595
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2002 The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1. Molecular cell 795 11931769
2008 Global analysis of host-pathogen interactions that regulate early-stage HIV-1 replication. Cell 787 18854154
2015 A cytoplasmic NF-κB interacting long noncoding RNA blocks IκB phosphorylation and suppresses breast cancer metastasis. Cancer cell 780 25759022
1999 IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain. The Journal of biological chemistry 727 10521409
1997 The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanism. Cell 721 9150141
1998 Structure of an IkappaBalpha/NF-kappaB complex. Cell 713 9865693
1997 CREB-binding protein/p300 are transcriptional coactivators of p65. Proceedings of the National Academy of Sciences of the United States of America 704 9096323
2003 Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA. Molecular cell 572 14690596
2018 Mechanisms of NF-κB p65 and strategies for therapeutic manipulation. Journal of inflammation research 544 30464573
2005 NF-kappaB RelA phosphorylation regulates RelA acetylation. Molecular and cellular biology 384 16135789
1991 Domain structure of synaptotagmin (p65). The Journal of biological chemistry 298 1985919
2005 Phosphorylation of RelA/p65 on serine 536 defines an I{kappa}B{alpha}-independent NF-{kappa}B pathway. The Journal of biological chemistry 291 16105840
2011 Nuclear factor p65 interacts with Keap1 to repress the Nrf2-ARE pathway. Cellular signalling 262 21262351
2009 p65/RelA modulates BECN1 transcription and autophagy. Molecular and cellular biology 219 19289499
1991 Structural and functional conservation of synaptotagmin (p65) in Drosophila and humans. The Journal of biological chemistry 206 1840599
2013 PRMT5 dimethylates R30 of the p65 subunit to activate NF-κB. Proceedings of the National Academy of Sciences of the United States of America 200 23904475
1996 Mutual transcriptional interference between RelA and androgen receptor. The Journal of biological chemistry 184 8798655
2010 Requirement of the NF-kappaB subunit p65/RelA for K-Ras-induced lung tumorigenesis. Cancer research 166 20406971
2003 Differential roles of RelA (p65) and c-Rel subunits of nuclear factor kappa B in tumor necrosis factor-related apoptosis-inducing ligand signaling. Cancer research 163 12615723
2006 IkappaB kinase alpha-mediated derepression of SMRT potentiates acetylation of RelA/p65 by p300. Molecular and cellular biology 159 16382138
2007 Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis. The Journal of clinical investigation 155 17525802
2008 RelA/p65 promotes osteoclast differentiation by blocking a RANKL-induced apoptotic JNK pathway in mice. The Journal of clinical investigation 143 18464930
1991 Differential expression of the p65 gene family. Neuron 135 2054189
2013 Resveratrol inhibits NF-kB signaling through suppression of p65 and IkappaB kinase activities. Die Pharmazie 132 24020126
2003 RelB forms transcriptionally inactive complexes with RelA/p65. The Journal of biological chemistry 126 12657634
2008 Nuclear factor-kappaB p65/relA silencing induces apoptosis and increases gemcitabine effectiveness in a subset of pancreatic cancer cells. Clinical cancer research : an official journal of the American Association for Cancer Research 114 19088029
2005 Nucleolar sequestration of RelA (p65) regulates NF-kappaB-driven transcription and apoptosis. Molecular and cellular biology 113 15988014
2009 Pim-1 controls NF-kappaB signalling by stabilizing RelA/p65. Cell death and differentiation 112 19911008
2011 Nucleolar NF-κB/RelA mediates apoptosis by causing cytoplasmic relocalization of nucleophosmin. Cell death and differentiation 110 21660047
2001 Protein phosphatase 2A interacts with and directly dephosphorylates RelA. The Journal of biological chemistry 110 11591705
2013 MicroRNA miR-324-3p induces promoter-mediated expression of RelA gene. PloS one 109 24265774
1988 The nucleotide sequence and characterization of the relA gene of Escherichia coli. The Journal of biological chemistry 105 2844820
2011 TNF-alpha induced NFκB signaling and p65 (RelA) overexpression repress Cldn5 promoter in mouse brain endothelial cells. Cytokine 103 22138107
2004 Pseudomonas aeruginosa relA contributes to virulence in Drosophila melanogaster. Infection and immunity 101 15385461
1996 Functional analysis of a relA/spoT gene homolog from Streptococcus equisimilis. Journal of bacteriology 100 8631718
1997 A new member of the I kappaB protein family, I kappaB epsilon, inhibits RelA (p65)-mediated NF-kappaB transcription. Molecular and cellular biology 91 9315679
1999 Cloning and characterization of a bifunctional RelA/SpoT homologue from Mycobacterium tuberculosis. Gene 90 10375643
2013 KPNB1, XPO7 and IPO8 mediate the translocation ofNF-κB/p65 into the nucleus. Traffic (Copenhagen, Denmark) 89 23906023
2008 Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65. Nucleic acids research 89 18263619
2007 Functions and regulation of NF-kappaB RelA during pneumococcal pneumonia. Journal of immunology (Baltimore, Md. : 1950) 88 17237440
2004 PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit. The Journal of biological chemistry 88 15140884
2007 Genetic inactivation of RelA/p65 sensitizes adult mouse hepatocytes to TNF-induced apoptosis in vivo and in vitro. Gastroenterology 84 17570221
2016 Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. Nature communications 82 27830706
1993 Casein kinase II phosphorylates the synaptic vesicle protein p65. The Journal of neuroscience : the official journal of the Society for Neuroscience 82 8463845
2013 Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells. Virology 77 24418562
2008 Nuclear factor kappa B signaling either stimulates or inhibits neurite growth depending on the phosphorylation status of p65/RelA. The Journal of neuroscience : the official journal of the Society for Neuroscience 77 18701687
2002 The p65/RelA subunit of NF-kappaB suppresses the sustained, antiapoptotic activity of Jun kinase induced by tumor necrosis factor. Molecular and cellular biology 76 12417721
2007 Cleavage of p65/RelA of the NF-kappaB pathway by Chlamydia. Proceedings of the National Academy of Sciences of the United States of America 74 17301240
2010 Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity. International journal of molecular medicine 71 21042790
2005 IKKbeta phosphorylates p65 at S468 in transactivaton domain 2. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 70 16046471
2003 Suppression of RelA/p65 nuclear translocation independent of IkappaB-alpha degradation by cyclooxygenase-2 inhibitor in gastric cancer. Oncogene 67 12606945
2021 ZFTA-RELA Dictates Oncogenic Transcriptional Programs to Drive Aggressive Supratentorial Ependymoma. Cancer discovery 66 33741710
1977 Analysis of the relA gene product of Escherichia coli. European journal of biochemistry 63 195816
2021 Transcriptional repression of NFKBIA triggers constitutive IKK- and proteasome-independent p65/RelA activation in senescence. The EMBO journal 61 33459422
2011 The measles virus V protein binds to p65 (RelA) to suppress NF-kappaB activity. Journal of virology 61 21270162
2010 Nucleolar targeting of RelA(p65) is regulated by COMMD1-dependent ubiquitination. Cancer research 58 20048074
2000 NF-kappaB p65 (RelA) homodimer uses distinct mechanisms to recognize DNA targets. Structure (London, England : 1993) 58 10801482
2015 Enterovirus 71 2C Protein Inhibits NF-κB Activation by Binding to RelA(p65). Scientific reports 56 26394554
2012 New molecular bridge between RelA/p65 and NF-κB target genes via histone acetyltransferase TIP60 cofactor. The Journal of biological chemistry 56 22249179
2015 Metalloprotease NleC suppresses host NF-κB/inflammatory responses by cleaving p65 and interfering with the p65/RPS3 interaction. PLoS pathogens 55 25756944
2006 Evidence for actin cytoskeleton-dependent and -independent pathways for RelA/p65 nuclear translocation in endothelial cells. The Journal of biological chemistry 55 17158457
2014 RETRACTED: Cyclophilin A (CypA) interacts with NF-κB subunit, p65/RelA, and contributes to NF-κB activation signaling. PloS one 54 25119989
2017 LRRC25 Functions as an Inhibitor of NF-κB Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Scientific reports 53 29044191
1989 Protein sequences encoded by the relA and the spoT genes of Escherichia coli are interrelated. The Journal of biological chemistry 53 2542299
2015 The NF-κB p65 and p50 homodimer cooperate with IRF8 to activate iNOS transcription. BMC cancer 52 26497740
2001 Functional analysis of relA and rshA, two relA/spoT homologues of Streptomyces coelicolor A3(2). Journal of bacteriology 52 11344157
2010 Involvement of the p65/RelA subunit of NF-kappaB in TNF-alpha-induced SIRT1 expression in vascular smooth muscle cells. Biochemical and biophysical research communications 50 20617556
2009 p65/RelA binds and activates the beclin 1 promoter. Autophagy 50 19458474
2019 TGF-β1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM. EBioMedicine 49 30926424
2006 Negative regulation of the RelA/p65 transactivation function by the product of the DEK proto-oncogene. The Journal of biological chemistry 49 16829531
2021 Supratentorial ependymoma in childhood: more than just RELA or YAP. Acta neuropathologica 48 33481105
2015 ComGA-RelA interaction and persistence in the Bacillus subtilis K-state. Molecular microbiology 47 25899641
2013 PPM1A is a RelA phosphatase with tumor suppressor-like activity. Oncogene 43 23812431
2017 PKCε phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation. Nature communications 41 29038521
2011 Variants of the RELA gene are associated with schizophrenia and their startle responses. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 41 21593732
2013 Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB. Oncotarget 40 23482348
2013 ASPP2 suppresses squamous cell carcinoma via RelA/p65-mediated repression of p63. Proceedings of the National Academy of Sciences of the United States of America 40 24127607
2020 Deamidation Shunts RelA from Mediating Inflammation to Aerobic Glycolysis. Cell metabolism 38 32325032
2010 PKC alpha phosphorylates cytosolic NF-kappaB/p65 and PKC delta delays nuclear translocation of NF-kappaB/p65 in U1242 glioblastoma cells. Turkish neurosurgery 38 20669099
2019 Hypoxia-induced RelA/p65 derepresses SLC16A3 (MCT4) by downregulating ZBTB7A. Biochimica et biophysica acta. Gene regulatory mechanisms 36 31271899
2015 A phosphomimetic mutant of RelA/p65 at Ser536 induces apoptosis and senescence: An implication for tumor-suppressive role of Ser536 phosphorylation. International journal of cancer 36 26375985
2022 OPA1 drives macrophage metabolism and functional commitment via p65 signaling. Cell death and differentiation 35 36307526
2014 Negative regulation of RelA phosphorylation: emerging players and their roles in cancer. Cytokine & growth factor reviews 35 25438737
2007 The oncoprotein gankyrin interacts with RelA and suppresses NF-kappaB activity. Biochemical and biophysical research communications 35 17904523
2015 ZBTB2 increases PDK4 expression by transcriptional repression of RelA/p65. Nucleic acids research 34 25609694
2010 BEX2 has a functional interplay with c-Jun/JNK and p65/RelA in breast cancer. Molecular cancer 33 20482821
2009 RelA regulates virulence and intracellular survival of Francisella novicida. Microbiology (Reading, England) 33 19762448
2018 MRI Phenotype of RELA-fused Pediatric Supratentorial Ependymoma. Clinical neuroradiology 32 30027327
2007 IEX-1 directly interferes with RelA/p65 dependent transactivation and regulation of apoptosis. Biochimica et biophysica acta 32 18191642
2012 Role of RelA and SpoT in Burkholderia pseudomallei virulence and immunity. Infection and immunity 30 22778096
2022 HTLV-1 activates YAP via NF-κB/p65 to promote oncogenesis. Proceedings of the National Academy of Sciences of the United States of America 29 35210364
2020 A Hyperactive RelA/p65-Hexokinase 2 Signaling Axis Drives Primary Central Nervous System Lymphoma. Cancer research 29 33067267
2019 Extracellular ATP activates P2X7R-NF-κB (p65) pathway to promote the maturation of bone marrow-derived dendritic cells of mice. Cytokine 29 30952064
2019 POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling. Cell death & disease 29 31723122
2005 Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies. BMC cancer 29 16332260
2019 Fatty acid starvation activates RelA by depleting lysine precursor pyruvate. Molecular microbiology 28 31400173
2016 Targeting NF-κB p65 with a Helenalin Inspired Bis-electrophile. ACS chemical biology 28 28103680
2010 6S RNA regulation of relA alters ppGpp levels in early stationary phase. Microbiology (Reading, England) 28 20829285
2020 Characterization of RelA in Acinetobacter baumannii. Journal of bacteriology 26 32229531
2003 Regulation of RelA (p65) function by the large subunit of replication factor C. Molecular and cellular biology 26 12509469
2023 Human RELA dominant-negative mutations underlie type I interferonopathy with autoinflammation and autoimmunity. The Journal of experimental medicine 25 37273177
2021 Ependymoma-like tumor with mesenchymal differentiation harboring C11orf95-NCOA1/2 or -RELA fusion: A hitherto unclassified tumor related to ependymoma. Brain pathology (Zurich, Switzerland) 25 33576087
2019 TLR4-RelA-miR-30a signal pathway regulates Th17 differentiation during experimental autoimmune encephalomyelitis development. Journal of neuroinflammation 25 31561751
2010 Thr435 phosphorylation regulates RelA (p65) NF-kappaB subunit transactivation. The Biochemical journal 25 20001970
2008 Differential RelA- and RelB-dependent gene transcription in LTbetaR-stimulated mouse embryonic fibroblasts. BMC genomics 25 19087315
2019 Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p. Experimental and therapeutic medicine 23 30906455