Affinage

STK26

Serine/threonine-protein kinase 26 · UniProt Q9P289

Length
416 aa
Mass
46.5 kDa
Annotated
2026-06-10
50 papers in source corpus 26 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 5/5 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

STK26 (MST4) is a Ste20-family serine/threonine kinase that regulates epithelial polarity, inflammatory signaling, and growth-control pathways through phosphorylation of a defined substrate set, and that acts as a context-dependent tumor suppressor or oncogene across tissues (PMID:11641781, PMID:19386264, PMID:32271880). Its catalytic output depends on an N-terminal kinase domain and a C-terminal regulatory domain, and full activation is achieved when MO25 binds and rotates the αC helix into an active conformation while the kinase domain homodimerizes to enable trans-autophosphorylation (PMID:11641781, PMID:11306563, PMID:23434407); activity is additionally gated by activation-loop phosphorylation at Thr178, imposed by PKA and by LIMK2 (PMID:26405038, PMID:40775397). Downstream of the LKB1/STRAD/MO25 polarity complex, MST4 translocates from the Golgi to the subapical membrane and phosphorylates Ezrin at Thr567 to drive brush border formation, and the same PKA–MST4–Ezrin/ACAP4 axis governs proton-pump translocation and gastric acid secretion (PMID:19386264, PMID:26405038, PMID:28808054). In immune and growth-control contexts MST4 phosphorylates TRAF6 (Thr463/Thr486) to block its oligomerization and limit NF-κB-driven cytokine production, restrains type I interferon by promoting Smurf1-dependent MAVS degradation, and feeds into Hippo and Wnt signaling by phosphorylating YAP at Thr83 to enforce cytoplasmic retention and β-catenin at Thr40 to protect it from GSK3β/β-TrCP-mediated degradation (PMID:25642822, PMID:35820905, PMID:32271880, PMID:34240584). Additional substrates and partners — ATG4B (Ser383) in autophagy, NPM1 (Thr95) in centrosome clustering, ALKBH5 (Ser64/Ser69) in stem-cell radioresistance, STAT1 in macrophage M1 polarization, and kinase-independent stabilization of p53 — extend its reach into tumorigenesis and tissue homeostasis (PMID:29232556, PMID:40775397, PMID:39990235, PMID:37833401, PMID:42204266).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2001 High

    Established MST4 as a catalytically active Ste20-related kinase with a two-domain architecture and an effector link to the ERK pathway, defining the protein and its first signaling output.

    Evidence In vitro kinase assay with kinase-dead mutant, dominant-negative epistasis, domain deletion, and yeast two-hybrid cloning via Raf

    PMID:11306563 PMID:11641781

    Open questions at the time
    • Physiological substrates beyond myelin basic protein not yet identified
    • Mechanism of ERK activation via MEK1 not resolved at molecular level
  2. 2007 High

    Identified PDCD10/CCM3 as a direct regulatory adaptor that boosts MST4 kinase activity and ERK-driven proliferation, providing the first activating partner.

    Evidence Yeast two-hybrid, reciprocal Co-IP, colocalization, in vitro kinase assay, siRNA

    PMID:17360971

    Open questions at the time
    • Structural basis of PDCD10-MST4 activation unknown
    • Relationship to MO25-mediated activation not delineated
  3. 2009 High

    Placed MST4 downstream of the LKB1/STRAD/MO25 polarity module and identified Ezrin Thr567 as the substrate driving brush border formation, defining a polarity-control function.

    Evidence Interaction assays, subcellular imaging/fractionation, in vitro kinase assay, dominant-negative morphological readout

    PMID:19386264

    Open questions at the time
    • Trigger for Golgi-to-membrane translocation not fully defined
    • Other apical substrates not yet enumerated
  4. 2011 High

    Showed MO25 directly binds and activates MST4 (3-4 fold), generalizing MO25 as a master activator of multiple STE20 kinases beyond LKB1.

    Evidence Biochemical binding, in vitro kinase activity, siRNA in mammalian cells

    PMID:21423148

    Open questions at the time
    • Differential roles of MO25α vs MO25β not resolved
  5. 2013 High

    Resolved the structural mechanism of activation: MO25 binding rotates the αC helix and kinase-domain homodimerization enables trans-autophosphorylation, with interface mutations confirming both requirements.

    Evidence X-ray crystallography, interface mutagenesis, in vitro kinase assays, apoptosis assay

    PMID:23434407

    Open questions at the time
    • How dimerization is regulated in cells unknown
    • Link between activation state and substrate selection unaddressed
  6. 2015 High

    Defined an anti-inflammatory function: MST4 phosphorylates TRAF6 at Thr463/Thr486 to block oligomerization and autoubiquitination, limiting cytokine production and protecting from septic shock.

    Evidence In vitro kinase assay, phospho-site mutagenesis, Co-IP, Traf6-/- epistasis, in vivo knockdown

    PMID:25642822

    Open questions at the time
    • Later osteoclast study reports opposite (pro-autoubiquitination) effect, indicating context-dependence not yet reconciled
  7. 2015 High

    Identified PKA-Thr178 phosphorylation as an upstream activation input and extended the Ezrin axis to gastric acid secretion via apical proton-pump translocation.

    Evidence In vitro kinase assay, phospho-deficient mutants, gastric acid secretion assay, parietal cell immunofluorescence

    PMID:26405038

    Open questions at the time
    • How PKA and MO25 inputs are integrated unknown
  8. 2017 High

    Expanded the substrate repertoire to ATG4B (Ser383) in autophagy and ACAP4 (Thr545) in gastric apical membrane reorganization, linking MST4 to autophagic flux/glioblastoma and to ARF6-GAP-dependent secretion.

    Evidence In vitro kinase assays, MS phospho-site mapping, knockdown/inhibitor, in vivo xenograft and acid secretion assays

    PMID:28808054 PMID:29232556

    Open questions at the time
    • Tissue-specific determinants of substrate choice not defined
  9. 2020 High

    Connected MST4 to Hippo signaling as a noncanonical YAP regulator: phosphorylation of YAP Thr83 blocks importin α binding and enforces cytoplasmic retention, with tumor-suppressive consequences in gastric tissue.

    Evidence In vitro kinase assay, phospho-mimetic epistasis, importin α binding assay, MST4 knockout mouse

    PMID:32271880

    Open questions at the time
    • Later studies report MST4-driven YAP activation in other tissues, indicating unresolved context-dependence
  10. 2021 High

    Defined a Wnt-axis function: MST4 phosphorylates β-catenin Thr40 to block GSK3β priming and β-TrCP-mediated degradation, controlling intestinal stem cell homeostasis and colorectal cancer.

    Evidence In vitro kinase assay, phospho-mimetic mutagenesis, GSK3β competition and β-TrCP binding assays, in vivo mouse models

    PMID:34240584

    Open questions at the time
    • Upstream signals controlling MST4 activity in ISCs not defined
  11. 2021 Medium

    Identified a metabolic role distinct from canonical substrate phosphorylation: MST4 localizes to hepatocyte lipid droplets and controls lipid accumulation by balancing β-oxidation, secretion, and lipogenesis.

    Evidence Subcellular localization, siRNA/overexpression with metabolic flux assays in human hepatocytes

    PMID:34278168

    Open questions at the time
    • Substrate(s) mediating lipid metabolic effects unidentified
    • Whether kinase activity is required not established
  12. 2022 Medium

    Extended the immune-suppressive role to antiviral signaling: MST4 competes with TRAF3 for MAVS and promotes Smurf1-dependent K48 ubiquitination/degradation of MAVS, dampening type I interferon.

    Evidence Co-IP, colocalization, IFN-β ELISA, ISG RT-PCR, siRNA/OE in infected cells

    PMID:35820905

    Open questions at the time
    • Single lab; reciprocal validation limited
    • Whether kinase activity is required not clearly resolved
  13. 2023 Medium

    Added STAT1 as a substrate driving macrophage M1 polarization and identified STRIPAK/GOLGA2 associations linking MST4 to STAT3 signaling in hepatocellular carcinoma.

    Evidence Phosphoproteomics, Co-IP, MS, macrophage-specific knockout mouse, siRNA functional assays

    PMID:37490000 PMID:37833401

    Open questions at the time
    • STAT1 phospho-site not mapped
    • Direct vs indirect STAT3 regulation not distinguished
  14. 2024 Medium

    Localized MST4 to the cardiomyocyte intercalated disc within STRIPAK complexes, where it promotes hypertrophy, contractility, and survival, with candidate disc substrates identified.

    Evidence Co-IP with STRIPAK, immunofluorescence localization, overexpression in rat cardiomyocytes, phosphoproteomics

    PMID:38579991

    Open questions at the time
    • Specific intercalated-disc substrates not validated
    • Loss-of-function cardiac phenotype not tested
  15. 2025 High

    Established a LIMK2-MST4-NPM1 cascade in which Thr178 activation enables NPM1 Thr95 phosphorylation to drive centrosome clustering and proliferation, and an MST4-USP14-ALKBH5 axis stabilizing ALKBH5 to enhance GBM radioresistance.

    Evidence In vitro kinase assays, phospho-site mutagenesis, Co-IP, ubiquitination assay, centrosome clustering readout, xenograft and carcinogenesis mouse models

    PMID:39990235 PMID:40775397

    Open questions at the time
    • How distinct upstream Thr178 inputs (PKA, LIMK2) select different downstream programs unknown
  16. 2026 Medium

    Defined kinase-independent and complex-based functions: MST4 stabilizes p53 by competing with MDM2, forms a 14-3-3ζ complex (structurally resolved) promoting YAP activation in pancreatic cancer, and its YAP regulation is countered by PPP4C through a MAP4K2-LATS1/2 cascade.

    Evidence CRISPR KO, Co-IP, ubiquitination/MDM2 competition assays, BioID, MST/ITC, X-ray crystallography, isograft and NSCLC models

    PMID:41690452 PMID:41981454 PMID:42204266

    Open questions at the time
    • Apparently opposite effects on YAP across tissues not mechanistically reconciled
    • Single-lab structural and functional claims await independent replication

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single kinase produces opposite tumor-suppressive vs oncogenic outcomes and activating vs inactivating YAP effects across tissues remains unresolved.
  • No unifying model for context-dependent substrate/effector selection
  • Determinants of tissue-specific localization (Golgi, lipid droplet, intercalated disc, apical membrane) not integrated
  • Relative contribution of kinase-dependent vs scaffolding functions unquantified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 10 GO:0016740 transferase activity 4 GO:0098772 molecular function regulator activity 2 GO:0140657 ATP-dependent activity 2
Localization
GO:0005886 plasma membrane 3 GO:0005794 Golgi apparatus 2 GO:0005811 lipid droplet 1 GO:0005829 cytosol 1
Pathway
R-HSA-1643685 Disease 5 R-HSA-162582 Signal Transduction 4 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-1430728 Metabolism 1 R-HSA-9612973 Autophagy 1
Partners
14-3-3ΖGOLGA2MAVSMO25MOB4PDCD10PPP4CTRAF6
Complex memberships
LKB1/STRAD/MO25 complexMST4-MOB4 complexSTRIPAK complex

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 MST4 is a novel Ste20-related serine/threonine kinase with an N-terminal kinase domain and unique C-terminal regulatory domain; wild-type but not kinase-dead MST4 phosphorylates myelin basic protein in vitro, and MST4 activates ERK via a Ras/Raf-1 independent pathway through MEK1. In vitro kinase assay with myelin basic protein substrate; dominant-negative MEK1 overexpression and PD98059 treatment; dominant-negative Ras/Raf-1 epistasis in transfected cells Oncogene High 11641781
2001 MST4 requires both its kinase and C-terminal regulatory domains for full kinase activation; an alternatively spliced isoform MST4a (lacking kinase subdomains IX–XI) may act as a dominant-negative regulator; MST4 was cloned via yeast two-hybrid interaction with the catalytic domain of Raf. Domain deletion analysis; Northern blot; yeast two-hybrid screening; fluorescence in situ hybridization for chromosomal localization to Xq26 The Journal of biological chemistry Medium 11306563
2007 PDCD10 (CCM3) directly interacts with MST4 (confirmed by co-immunoprecipitation and colocalization), increases MST4 kinase activity in vitro, and acts as a regulatory adaptor for MST4-mediated ERK pathway activation promoting cell proliferation and transformation. Yeast two-hybrid screening; co-immunoprecipitation; colocalization assays; in vitro kinase assay; siRNA knockdown with ERK activity readout Molecular biology of the cell High 17360971
2009 MST4 acts downstream of the LKB1/STRAD/MO25 polarization complex; Mo25α directly interacts with MST4, and upon LKB1 activation, MST4 translocates from the Golgi to the subapical membrane compartment. MST4 phosphorylates the regulatory T567 residue of Ezrin, which is essential for brush border formation; inhibition of MST4 blocks brush border formation without affecting lateral junction formation. Protein interaction assays; live-cell imaging/subcellular fractionation; in vitro kinase assay for Ezrin T567 phosphorylation; dominant-negative MST4 inhibition with morphological readout Developmental cell High 19386264
2011 MO25α and MO25β bind to MST4 (and MST3/YSK1) in a manner similar to STRAD binding, stimulating MST4 kinase activity approximately 3–4-fold; MO25 is thus a master activator of multiple STE20-family kinases beyond LKB1 regulation. Biochemical binding assays; in vitro kinase activity measurements; siRNA knockdown of MO25 in mammalian cells The EMBO journal High 21423148
2013 Crystal structure of MST4 in complex with MO25 reveals that MO25 binding rotates the αC helix of MST4 into an active conformation. The MST4 kinase domain forms a specific homodimer required for trans-autophosphorylation. Interface mutations disrupting either MST4-MO25 interaction or kinase-domain homodimerization impair MST4 kinase activation and function. MO25-stimulated MST4 promotes apoptosis in HEK293T cells. X-ray crystallography; site-directed mutagenesis of interface residues; in vitro kinase assays; cell-based apoptosis assay Structure High 23434407
2015 MST4 directly phosphorylates TRAF6 at Thr463 and Thr486 to prevent TRAF6 oligomerization and autoubiquitination, thereby limiting LPS-induced inflammatory cytokine production. Mutation of TRAF6 at these sites abrogates MST4-mediated inhibition. MST4 knockdown exacerbates septic shock in mice, an effect rescued by heterozygous Traf6 deletion. In vitro kinase assay; site-directed mutagenesis of TRAF6 phospho-acceptor sites; co-immunoprecipitation; genetic epistasis in Traf6-/- fibroblasts; in vivo MST4 knockdown mouse model Nature immunology High 25642822
2015 PKA phosphorylates MST4 at Thr178, activating its kinase activity. Activated MST4 then phosphorylates Ezrin at Thr567 (in addition to PKA phosphorylating Ezrin at Ser66), and this PKA-MST4-Ezrin signaling cascade is required for histamine-elicited acid secretion and apical membrane reorganization in gastric parietal cells. Non-phosphorylatable MST4 or Ezrin mutants block proton pump translocation. In vitro kinase assay; site-directed mutagenesis; overexpression of phospho-deficient mutants; gastric acid secretion functional assay; immunofluorescence of parietal cell membranes The Journal of biological chemistry High 26405038
2015 The MST4 crystal structure ATP-binding site was used for virtual docking to identify hesperadin as a potent nanomolar inhibitor of MST4 kinase activity; hesperadin blocks MST4-mediated protection from hypoxia-induced apoptosis and proliferation in pituitary gonadotrope cells. Structure-based virtual screening; TR-FRET in vitro recombinant kinase assay; cell-based apoptosis and proliferation assays with hypoxia model Molecular cancer therapeutics Medium 26721946
2017 MST4 (STK26) phosphorylates ATG4B at Ser383, which stimulates ATG4B activity and increases autophagic flux. Inhibition of MST4 or ATG4B suppresses autophagy and tumorigenicity of glioblastoma cells. Radiation induces MST4 expression and ATG4B phosphorylation. In vitro kinase assay identifying specific phosphorylation site; genetic knockdown/inhibitor studies with autophagic flux readout; in vivo intracranial xenograft model with radiotherapy combination Cancer cell High 29232556
2017 MST4 phosphorylates ACAP4 (an ARF6 GTPase-activating protein) at Thr545, which is essential for apical membrane reorganization and H,K-ATPase proton pump translocation during histamine-elicited gastric acid secretion. Phosphorylation of Thr545 enables ACAP4 interaction with Ezrin, and MST4-ACAP4 interaction is promoted by histamine stimulation. In vitro kinase assay; mass spectrometric phosphorylation site mapping; co-immunoprecipitation; overexpression of non-phosphorylatable ACAP4 mutant; gastric acid secretion functional assay The Journal of biological chemistry High 28808054
2018 MST4 forms a phosphorylation-dependent complex with MOB4; the MST4-MOB4 complex has an overall structure resembling the MST1-MOB1 complex but exhibits opposite (pro-oncogenic) biological function. MST4-MOB4 can disrupt the MST1-MOB1 complex through alternative pairing due to divergent evolution of interface residues, thereby increasing YAP activity. Co-immunoprecipitation; structural analysis; cell growth and migration assays with PANC-1 cells; competitive binding experiments The Journal of biological chemistry Medium 30072378
2020 MST4 directly phosphorylates YAP at Thr83, which blocks YAP binding to importin α, leading to YAP cytoplasmic retention and inactivation. This MST4-YAP axis is a noncanonical Hippo pathway; T83E YAP mutation mimicking MST4 phosphorylation restrains both wild-type YAP and its S127A mutant activity. MST4 depletion in mice promotes gastric tumorigenesis with YAP hyperactivation. In vitro kinase assay; site-directed mutagenesis (T83E phospho-mimetic); importin α binding assay; MST4 knockout mouse model with tumorigenesis readout The Journal of experimental medicine High 32271880
2021 MST4 directly phosphorylates β-catenin at Thr40, blocking its Ser33 phosphorylation by GSK3β and thereby preventing β-TrCP-mediated degradation and leading to β-catenin accumulation and full activation. This MST4-pβ-cateninThr40 axis is required for intestinal stem cell homeostasis; mice with MST4T178E (constitutively active) or β-cateninT40D mutations show excess ISCs/CSCs and exacerbated CRC. In vitro kinase assay; phospho-site mutagenesis (T40D phospho-mimetic); GSK3β phosphorylation competition assay; β-TrCP binding assay; MST4 depletion with ISC phenotype readout; in vivo mouse models Advanced science High 34240584
2022 MST4 negatively regulates type I interferon production by competing with TRAF3 for binding to the 360-540 domain of MAVS, and by facilitating Smurf1-MAVS interaction, thereby promoting K48-linked ubiquitination and proteasomal degradation of MAVS. Co-immunoprecipitation; immunofluorescence colocalization; ELISA for IFN-β; RT-PCR for IFN-stimulated genes; siRNA knockdown and overexpression in virus-infected cells Cell communication and signaling Medium 35820905
2023 MST4 directly phosphorylates STAT1, and this phosphorylation is essential for M1 polarization of macrophages; MST4 knockdown directly inhibits STAT1 phosphorylation, reduces M1 macrophage markers and cytokines, and impairs phagocytosis. Macrophage-specific Mst4 knockout in mice attenuates ITP pathology. Co-immunoprecipitation; mass spectrometry; phosphoproteomics; macrophage-specific Mst4 knockout mouse model; immunofluorescence; RNA-seq Cellular & molecular immunology Medium 37833401
2023 MST3 and MST4 interact with GOLGA2 (GM130) and STRIPAK complex components in hepatocytes; silencing of MST4 markedly suppressed tumorigenesis and identified lower STAT3 signaling activation in MST3/MST4-deficient HCC cells. Co-immunoprecipitation identifying GOLGA2 and STRIPAK as binding partners; siRNA silencing with proliferation/migration/invasion/EMT readouts; public dataset analysis FASEB journal Medium 37490000
2024 MST4 associates with STRIPAK complex components in cardiomyocytes and localizes to the intercalated disc, interacting with intercalated disc proteins. MST4 overexpression induces cardiomyocyte hypertrophy, increases sarcomeric fractional shortening (contractility), and inhibits apoptosis (reduced cleaved caspase3, caspase7, and PARP1). Phosphoproteomics identified novel MST4 target candidates at the intercalated disc. Co-immunoprecipitation with STRIPAK components; immunofluorescence localization to intercalated disc; overexpression in adult rat cardiomyocytes with contractility and apoptosis readouts; phosphoproteomics The Journal of biological chemistry Medium 38579991
2025 MST4 phosphorylates ALKBH5 at Ser64 and Ser69, which increases ALKBH5 interaction with the deubiquitinase USP14, promoting ALKBH5 deubiquitylation and stabilization (preventing HECW2-mediated K48-linked ubiquitination). This MST4-USP14-ALKBH5 axis enhances GBM stem cell radioresistance and homologous recombination repair. Mass spectrometry; co-immunoprecipitation; phospho-site mutagenesis; ubiquitination assay; shRNA knockdown with radioresistance and xenograft readouts Theranostics Medium 39990235
2025 LIMK2 phosphorylates MST4 at Thr178, activating its kinase function; activated MST4 then binds and phosphorylates nucleophosmin 1 (NPM1) at Thr95, a modification essential for centrosome clustering and tumor cell proliferation. In vitro kinase assay; site-directed mutagenesis; co-immunoprecipitation; NPM1 siRNA knockdown with centrosome clustering readout; in vivo xenograft and 4NQO carcinogenesis mouse models Oncogene High 40775397
2026 MST4 directly interacts with p53 protein and competes with MDM2 to prevent K48-linked ubiquitination and degradation of both wild-type and gain-of-function mutant p53, thereby stabilizing p53 in a kinase-independent manner. CRISPR-generated MST4 knockout cell lines; co-immunoprecipitation; ubiquitination assay; MDM2 competition assay; xenograft model Oncogene Medium 42204266
2026 MST4 forms a phosphorylation-dependent complex with 14-3-3ζ, leading to YAP activation and enhanced pancreatic cancer cell migration; X-ray crystallographic structural analysis revealed the MST4–14-3-3ζ complex interface. Peptide inhibitors disrupting this interaction suppressed YAP activation and tumor growth in vitro and in vivo. BioID proximity labeling; microscale thermophoresis; isothermal titration calorimetry; X-ray crystallography; cell migration assay; xenograft mouse model Biology direct High 41981454
2025 MST4 (STK26) interacts with p50ATF6 and enhances its protein stabilization, activating the ATF6 unfolded protein response signaling branch to promote colorectal cancer cell growth and migration; MST4's oncogenic function depends on ATF6 as shown by ATF6 inhibitor reversal. Co-immunoprecipitation; luciferase reporter assay for ATF6 pathway activation; transcriptome sequencing; tumor phenotype assays; in vivo hesperadin (STK26 inhibitor) treatment International journal of molecular sciences Medium 40869379
2025 MST4 promotes TRAF6 autoubiquitination through phosphorylation in osteoclasts, a critical event for osteoclast activation. MST4 knockdown reduced osteoclast differentiation and bone resorption; MST4 overexpression enhanced these processes. In vivo ovariectomized mouse models corroborated these findings. In vitro osteoclast differentiation assay; siRNA knockdown and overexpression; bone resorption assay; ubiquitination assay; ovariectomized mouse model Journal of pharmaceutical analysis Medium 41940169
2021 MST4 protein is predominantly associated with intracellular lipid droplets in human and rodent hepatocytes; MST4 silencing attenuates lipid accumulation by stimulating β-oxidation and triacylglycerol secretion, while inhibiting fatty acid influx and lipogenesis; MST4 overexpression has the opposite effects, also affecting oxidative/ER stress. Subcellular fractionation/immunofluorescence identifying lipid droplet localization; siRNA silencing and OE with metabolic flux assays (β-oxidation, TG secretion, lipogenesis); human hepatocyte primary culture Hepatology communications Medium 34278168
2026 PPP4C (protein phosphatase 4 catalytic subunit) interacts with MST4 and reduces its function; MST4 induces phosphorylation and cytoplasmic degradation of YAP1 by influencing the MAP4K2-LATS1/2 cascade, and PPP4C counteracts this to promote YAP1 activation in NSCLC. Co-immunoprecipitation; gain/loss-of-function in NSCLC cell lines; phosphorylation and YAP localization assays; in vivo isograft tumor models; NK cell co-culture immune assay Cancer letters Medium 41690452

Source papers

Stage 0 corpus · 50 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma. Cancer cell 202 29232556
2007 PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway. Molecular biology of the cell 134 17360971
2009 Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25 polarization complex. Developmental cell 133 19386264
2011 MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases. The EMBO journal 122 21423148
2001 MST4, a new Ste20-related kinase that mediates cell growth and transformation via modulating ERK pathway. Oncogene 92 11641781
2015 The kinase MST4 limits inflammatory responses through direct phosphorylation of the adaptor TRAF6. Nature immunology 89 25642822
2001 Cloning and characterization of MST4, a novel Ste20-like kinase. The Journal of biological chemistry 65 11306563
2020 MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway. The Journal of experimental medicine 62 32271880
2018 The MST4-MOB4 complex disrupts the MST1-MOB1 complex in the Hippo-YAP pathway and plays a pro-oncogenic role in pancreatic cancer. The Journal of biological chemistry 59 30072378
2003 The Ste20 kinase MST4 plays a role in prostate cancer progression. Cancer research 47 12810671
2023 MST4 kinase regulates immune thrombocytopenia by phosphorylating STAT1-mediated M1 polarization of macrophages. Cellular & molecular immunology 40 37833401
2014 MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway. International journal of oncology 39 24859810
2013 Structure of the MST4 in complex with MO25 provides insights into its activation mechanism. Structure (London, England : 1993) 39 23434407
2020 Neratinib degrades MST4 via autophagy that reduces membrane stiffness and is essential for the inactivation of PI3K, ERK1/2, and YAP/TAZ signaling. Journal of cellular physiology 28 31912905
2015 Cell Polarity Kinase MST4 Cooperates with cAMP-dependent Kinase to Orchestrate Histamine-stimulated Acid Secretion in Gastric Parietal Cells. The Journal of biological chemistry 24 26405038
2014 Differential expression of MST4, STK25 and PDCD10 between benign prostatic hyperplasia and prostate cancer. International journal of clinical and experimental pathology 24 25550858
2021 MST4 negatively regulates the EMT, invasion and metastasis of HCC cells by inactivating PI3K/AKT/Snail1 axis. Journal of Cancer 23 34149910
2021 An MST4-pβ-CateninThr40 Signaling Axis Controls Intestinal Stem Cell and Tumorigenesis. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 23 34240584
2015 Structure-Based Screen Identification of a Mammalian Ste20-like Kinase 4 (MST4) Inhibitor with Therapeutic Potential for Pituitary Tumors. Molecular cancer therapeutics 21 26721946
2017 MST4 kinase phosphorylates ACAP4 protein to orchestrate apical membrane remodeling during gastric acid secretion. The Journal of biological chemistry 20 28808054
2025 USP14 modulates stem-like properties, tumorigenicity, and radiotherapy resistance in glioblastoma stem cells through stabilization of MST4-phosphorylated ALKBH5. Theranostics 18 39990235
2020 MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice. Behavioural neurology 18 32089749
2020 MST4 inhibits human hepatocellular carcinoma cell proliferation and induces cell cycle arrest via suppression of PI3K/AKT pathway. Journal of Cancer 17 32742458
2021 STE20-Type Protein Kinase MST4 Controls NAFLD Progression by Regulating Lipid Droplet Dynamics and Metabolic Stress in Hepatocytes. Hepatology communications 15 34278168
2019 MicroRNA-486-5p inhibits ovarian granulosa cell proliferation and participates in the development of PCOS via targeting MST4. European review for medical and pharmacological sciences 14 31539108
2023 The mammalian Sterile 20-like kinase 4 (MST4) signaling in tumor progression: Implications for therapy. Cancer letters 13 37094736
2023 STE20-type kinases MST3 and MST4 promote the progression of hepatocellular carcinoma: Evidence from human cell culture and expression profiling of liver biopsies. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 10 37490000
2022 MST4: A Potential Oncogene and Therapeutic Target in Breast Cancer. Cells 10 36552828
2023 MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy. Heliyon 8 37313160
2019 MST4 modulates the neuro-inflammatory response by regulating IκBα signaling pathway and affects the early outcome of experimental ischemic stroke in mice. Brain research bulletin 8 31722252
2022 MST4 negatively regulates type I interferons production via targeting MAVS-mediated pathway. Cell communication and signaling : CCS 7 35820905
2020 Downregulation of MST4 Underlies a Novel Inhibitory Role of MicroRNA Let-7a in the Progression of Retinoblastoma. Investigative ophthalmology & visual science 7 32539131
2024 Genetic Ablation of STE20-Type Kinase MST4 Does Not Alleviate Diet-Induced MASLD Susceptibility in Mice. International journal of molecular sciences 6 38397122
2024 Mst4, a novel cardiac STRIPAK complex-associated kinase, regulates cardiomyocyte growth and survival and is upregulated in human cardiomyopathy. The Journal of biological chemistry 6 38579991
2017 Molecular cloning, characterization and function of a germinal center kinase MST4 gene from Litopenaeus vannamei in response to Vibrio alginolyticus challenge in TLR-TRAF6 signaling pathway. Developmental and comparative immunology 6 28377200
2022 MST4 as a novel therapeutic target for autophagy and radiosensitivity in gastric cancer. IUBMB life 5 36239138
2020 MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression. OncoTargets and therapy 5 33244239
2024 Targeting STK26 and ATG4B: miR-22-3p as a modulator of autophagy and tumor progression in HCC. Translational oncology 4 39608212
2012 Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4. Acta crystallographica. Section F, Structural biology and crystallization communications 4 22750858
2020 Tripartite motif containing 59 (TRIM59) promotes esophageal cancer progression via promoting MST4 expression and ERK pathway. Journal of receptor and signal transduction research 3 32340525
2004 [Fine mapping of Smith-Fineman-Myers syndrome and exclusion of GPC3, GPCR2 MST4 and GLUD2 as candidate genes]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 2 15192816
2023 Tandem mass tag (TMT) quantitative protein analysis-based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity. Molecular carcinogenesis 1 37378424
2017 MST-4 and TRAF-6 expression in the peripheral blood mononuclear cells of patients with Graves' disease and its significance. BMC endocrine disorders 1 28219358
2026 PPP4C restores YAP1 activity by modulating MST4 phosphorylation to enhance immunosuppression and augment tumor growth in non-small cell lung cancer. Cancer letters 0 41690452
2026 MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression [Retraction]. OncoTargets and therapy 0 41710904
2026 The MST4-14-3-3ζ complex promotes pancreatic cancer by activating YAP. Biology direct 0 41981454
2026 MST4 plays dual roles in lung adenocarcinoma by regulating homeostasis of wild-type and mutant p53 protein. Oncogene 0 42204266
2025 LIMK2 promotes centrosome clustering and cancer progression by activating MST4-mediated phosphorylation of NPM1. Oncogene 0 40775397
2025 STK26 Promotes the Stabilization of ATF6 to Facilitate the Progression of Colorectal Cancer. International journal of molecular sciences 0 40869379
2025 MST4 as a key driver of osteoclast activation in osteoporosis. Journal of pharmaceutical analysis 0 41940169

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