Affinage

STK25

Serine/threonine-protein kinase 25 · UniProt O00506

Length
426 aa
Mass
48.1 kDa
Annotated
2026-06-10
48 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

STK25 (YSK1/SOK1) is a STE20-family serine/threonine kinase that integrates Golgi organization, cell polarity, Hippo signaling, and metabolic and inflammatory control (PMID:15037601, PMID:30948712). Its catalytic activity, intrinsic to the kinase domain (PMID:9160885), is activated by direct binding to the Golgi matrix protein GM130, which promotes autophosphorylation of a conserved T-loop threonine and recruits the kinase to the Golgi where it phosphorylates 14-3-3ζ (PMID:15037601), and is further stimulated by the scaffold protein MO25α/β (PMID:21423148). Through an LKB1-STRAD-STK25-GM130 axis and kinase-independent assembly with α/β-PIX and the Cullin3-Bacurd1 RhoA-ubiquitination complex, STK25 controls Golgi morphology, neuronal polarization, and cortical neuronal migration by balancing Rac1 and RhoA activity, acting antagonistically to Reelin-Dab1 signaling (PMID:21111240, PMID:24225308, PMID:34518307). In Hippo signaling STK25 promotes LATS activation-loop phosphorylation independently of hydrophobic-motif phosphorylation to suppress YAP/TAZ, and as the kinase subunit of STRIPAK it phosphorylates SAV1 to relieve SAV1-mediated inhibition of the STRIPAK phosphatase and restrain MST2 (PMID:30948712, PMID:32292165). STK25 additionally phosphorylates the PKA regulatory subunit PRKAR1A to inhibit PKA activity (PMID:35977512) and phosphorylates IRF5 at Thr265 to drive TLR-induced pro-inflammatory cytokine production (PMID:40639948). At intrahepatic lipid droplets STK25 coats the droplet surface, displaces ATGL, and acts as a negative regulator of β-oxidation and glucose uptake, such that its loss improves insulin sensitivity and reduces ectopic lipid accumulation (PMID:25609431, PMID:25845663, PMID:22391949). A caspase-cleaved STK25 fragment translocates from the Golgi to the nucleus to execute apoptosis after anoxic/oxidative stress (PMID:18364353).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1997 Medium

    Establishing that STK25 is a catalytically active STE20-related kinase distinct from canonical MAPK cascades defined it as an independent signaling node rather than an upstream MAPKKK.

    Evidence cDNA cloning with immunoprecipitation kinase assay and MAPK reporter assays after overexpression

    PMID:9160885

    Open questions at the time
    • No physiological substrate identified
    • No localization or activation mechanism defined
  2. 2004 High

    Identifying GM130-dependent Golgi targeting and T-loop autophosphorylation, plus 14-3-3ζ as a substrate, explained where and how STK25 is activated and linked it to Golgi organization and cell migration.

    Evidence Co-IP, in vitro kinase assays, biochemical substrate screen, and migration/invasion assays in cells

    PMID:15037601

    Open questions at the time
    • Functional consequence of 14-3-3ζ phosphorylation unresolved
    • Mechanism linking Golgi position to migration not detailed
  3. 2008 Medium

    Showing that a caspase-cleaved STK25 fragment must enter the nucleus to drive apoptosis after anoxia revealed a stress-responsive pro-death function gated by proteolysis and a defined C-terminal segment.

    Evidence siRNA, nuclear-localization mutants, fractionation/imaging and caspase-inhibitor apoptosis assays

    PMID:18364353

    Open questions at the time
    • Nuclear substrates of the cleaved fragment unknown
    • Single lab; connection to Golgi pool unclear
  4. 2010 High

    Placing STK25 within an LKB1-STK25-GM130 pathway opposing Reelin-Dab1 connected its Golgi function to neuronal polarization in vivo.

    Evidence Gain/loss-of-function in cultured neurons with Reelin/Dab1 mouse genetics and Golgi/axon imaging

    PMID:21111240

    Open questions at the time
    • Direct kinase substrates in this pathway not defined
    • How Golgi condensation generates supernumerary axons unresolved
  5. 2011 High

    Demonstrating that MO25 directly binds and stimulates STK25 kinase activity identified a second activator parallel to the LKB1-STRAD-MO25 mechanism.

    Evidence SPR binding, co-IP, in vitro kinase assays with recombinant proteins, and cellular MO25 knockdown

    PMID:21423148

    Open questions at the time
    • Cellular context where MO25 vs GM130 dominates not defined
    • Substrate selectivity changes upon MO25 binding unknown
  6. 2012 High

    Identifying CCM3/PDCD10 stabilization and CCM2 phosphorylation embedded STK25 in CCM-protein and TrkA death-signaling networks with a catalytic requirement.

    Evidence AP-MS, co-IP, in vitro kinase assays, kinase-dead mutants, proteasome inhibitors and cell-death rescue

    PMID:22652780 PMID:22782892

    Open questions at the time
    • Functional consequence of CCM2 phosphorylation not defined
    • Reconciliation of pro-survival and pro-death roles incomplete
  7. 2012 Medium

    A modifier screen confirming STK25 antagonism to Dab1 and its control of Tau phosphorylation reinforced its role in neuronal polarization and Golgi morphology.

    Evidence Microarray modifier screen in dab1-null mice with siRNA validation and Tau/Golgi readouts

    PMID:22355340

    Open questions at the time
    • Direct kinase target driving Tau phosphorylation not identified
    • Mechanism is correlative for some readouts
  8. 2013 Medium

    Loss-of-function in muscle cells and knockout mice established STK25 as a negative regulator of lipid oxidation, glucose uptake, and gluconeogenesis, with ACC as a candidate effector.

    Evidence siRNA in L6 myoblasts and Stk25 knockout mice with metabolic phenotyping and clamp studies

    PMID:22391949 PMID:25845663

    Open questions at the time
    • Direct substrate linking STK25 to ACC unknown
    • Tissue-specific mechanisms not fully separated
  9. 2013 Medium

    Conditional knockout phenocopied by knockdown of pathway members confirmed STK25's requirement for cortical neuronal migration within the LKB1-STRAD-GM130 axis.

    Evidence Cre knockout, siRNA, in utero electroporation and migration assays in developing brain

    PMID:24225308

    Open questions at the time
    • Molecular effectors of migration downstream of STK25 not defined here
  10. 2015 Medium

    Localizing STK25 to hepatic lipid droplets and showing ATGL displacement gave a physical mechanism for its promotion of steatosis.

    Evidence Transgenic mice, colocalization, fractionation, β-oxidation and TAG secretion assays

    PMID:25609431

    Open questions at the time
    • Whether ATGL displacement is kinase-dependent unresolved
    • Direct droplet substrates not identified
  11. 2018 Medium

    Linking STK25 to GOLPH3-mTOR signaling and aerobic glycolysis suppression positioned it as a tumor-suppressive metabolic regulator in colorectal cancer.

    Evidence Co-IP, GST/His pull-downs, mTOR western blots, glycolysis assays and xenografts

    PMID:29996891

    Open questions at the time
    • Whether STK25 phosphorylates GOLPH3 not established
    • Single lab
  12. 2019 High

    Mechanistic dissection of LATS activation-loop phosphorylation independent of hydrophobic-motif phosphorylation defined how STK25 activates Hippo signaling and restrains YAP/TAZ-driven proliferation.

    Evidence siRNA/CRISPR, phospho-site-specific antibodies, YAP/TAZ reporters, proliferation assays and cancer genomics

    PMID:30948712

    Open questions at the time
    • Direct vs indirect phosphorylation of LATS not fully resolved
    • Relationship to STRIPAK function not addressed here
  13. 2019 Medium

    Lipid-droplet phosphoproteomics tied STK25 to peroxisomal biogenesis and antioxidant/ER-stress defense in a hepatocyte-autonomous manner.

    Evidence Stk25 KO mice phosphoproteomics and human liver-cell siRNA with stress markers

    PMID:31857389

    Open questions at the time
    • Direct droplet substrates not validated individually
    • Causal phosphosite for peroxisomal effects unknown
  14. 2020 High

    Demonstrating SAV1 phosphorylation by STK25 within STRIPAK clarified a second, opposing arm of STK25 Hippo control, where it relieves SAV1 inhibition of the STRIPAK phosphatase to restrain MST2.

    Evidence siRNA, in vitro kinase assay on SAV1, STRIPAK integrity co-IP and MST2 activation assays

    PMID:32292165

    Open questions at the time
    • Reconciliation of LATS-activating and MST2-restraining roles incomplete
    • Context determining which arm dominates unknown
  15. 2021 Medium

    Showing kinase-independent control of Rac1/RhoA via PIX and Cullin3-Bacurd1 complexes revealed a non-catalytic scaffolding mechanism for STK25 in neuronal migration.

    Evidence Conditional KO, in utero electroporation, co-IP, Rho GTPase activity assays and MST3 rescue

    PMID:34518307

    Open questions at the time
    • Structural basis of PIX/Cul3 complex assembly unknown
    • Interplay with kinase-dependent functions undefined
  16. 2022 High

    Identifying PRKAR1A as a substrate whose phosphorylation inhibits PKA connected STK25 to cAMP/PKA signaling and cardiac contractility.

    Evidence iPSC-cardiomyocytes, in vitro kinase assay, regulatory-catalytic subunit co-IP, KO mice and contractility measurements

    PMID:35977512

    Open questions at the time
    • Phosphosite on PRKAR1A not specified here
    • Tissue breadth of PKA regulation not mapped
  17. 2022 Medium

    Combined STK24/STK25 deficiency producing cavernoma-like lesions with KLF2 and β-catenin/Golgi changes implicated STK25 in vascular integrity, partly redundant with STK24.

    Evidence Double KO mice and endothelial siRNA with histology and immunofluorescence

    PMID:35130716

    Open questions at the time
    • Substrate driving endothelial phenotype not identified
    • Mechanistic link to CCM-protein interactions not closed
  18. 2022 Medium

    Linking STK25 loss to NEDD4-dependent K48-ubiquitination of PD-L1 connected the kinase to tumor immune evasion.

    Evidence KO cells/mice, co-IP, K48-linkage ubiquitination assays and anti-PD-1 tumor models

    PMID:40729594

    Open questions at the time
    • Whether STK25 directly phosphorylates NEDD4 or PD-L1 unresolved
    • Single lab
  19. 2025 Medium

    Identifying IRF5-Thr265 as a substrate established STK25 as a TLR-responsive kinase driving IRF5-dependent inflammatory cytokine production.

    Evidence Kinome-wide siRNA screen, in vitro kinase assay, KO primary immune cells, IRF5 translocation and cytokine ELISA

    PMID:40639948

    Open questions at the time
    • Upstream signal activating STK25 in TLR pathway not defined
    • Single lab
  20. 2026 Medium

    Linking STK25 loss to NF-κB/p50-driven AREG transcription and EGFR activation on fibroblasts explained a mechanism of cetuximab resistance in colorectal cancer.

    Evidence siRNA/overexpression, ChIP, luciferase, CAF co-culture, xenografts and patient-derived organoids

    PMID:42057436

    Open questions at the time
    • Direct kinase substrate connecting STK25 to NF-κB unknown
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how STK25's many context-specific functions are coordinated by a single kinase, and how activation inputs select among its diverse substrates.
  • No unifying substrate-selection mechanism defined
  • Tissue- and stimulus-specific activator usage not mapped
  • Structural basis of kinase-dependent vs scaffolding functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 5 GO:0140096 catalytic activity, acting on a protein 5 GO:0140110 transcription regulator activity 1
Localization
GO:0005794 Golgi apparatus 4 GO:0005811 lipid droplet 2 GO:0005634 nucleus 1
Pathway
R-HSA-1430728 Metabolism 4 R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-168256 Immune System 1
Partners
ARHGEF6 (ALPHA-PIX)CAB39 (MO25)CCM2CUL3GM130GOLPH3PDCD10SAV1
Complex memberships
STRIPAK

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 YSK1 (STK25) targets to the Golgi apparatus via direct binding to the Golgi matrix protein GM130, and GM130 binding activates YSK1 by promoting autophosphorylation of a conserved threonine within the T-loop. A biochemical screen identified 14-3-3zeta as a specific substrate for YSK1 phosphorylation at the Golgi. Interference with YSK1 function perturbs perinuclear Golgi organization, cell migration, and invasion into type I collagen. Co-immunoprecipitation, in vitro kinase assays, biochemical substrate screen, dominant-negative interference, cell migration/invasion assays The Journal of cell biology High 15037601
2010 Stk25 functions as part of an LKB1-Stk25-GM130 signaling pathway that regulates Golgi morphology and neuronal polarization. Overexpression of Stk25 induces Golgi condensation and multiple axons (supernumerary axons), both of which are rescued by Reelin treatment, placing STK25 in opposition to Reelin-Dab1 signaling. Reelin-Dab1 promotes extension of the Golgi into dendrites, which is suppressed by Stk25 overexpression. Overexpression and knockdown in cultured neurons, in vivo mouse genetics (Reelin and Dab1 mutants), Golgi morphology imaging, axon/dendrite quantification Cell High 21111240
2011 MO25α/β isoforms directly bind to STK25/YSK1 (and MST3/MST4) and stimulate their kinase activity approximately 3- to 4-fold, in a manner analogous to how MO25 activates LKB1 via STRAD. Binding assays (surface plasmon resonance, co-immunoprecipitation), in vitro kinase activity assays with recombinant proteins, siRNA knockdown of MO25 in cells The EMBO journal High 21423148
2008 SOK1 (STK25) regulates apoptotic cell death after chemical anoxia via the intrinsic apoptotic pathway. A caspase-cleaved form of SOK1 translocates from the Golgi to the nucleus after anoxia, dependent on caspase activity and on amino acids 275-292 C-terminal to the kinase domain. Nuclear entry of SOK1 is required for the cell death response, as SOK1 mutants unable to enter the nucleus do not induce cell death. RNA interference (siRNA knockdown), overexpression of wild-type and nuclear-localization mutants, subcellular fractionation/imaging, apoptosis assays, caspase inhibitor experiments The Journal of biological chemistry Medium 18364353
1997 YSK1 (STK25) encodes a novel mammalian Ste20-related serine/threonine kinase with intrinsic protein kinase activity detectable by immunoprecipitation kinase assay. Overexpression of YSK1 does not activate the ERK, JNK/SAPK, or p38 MAPK pathways. cDNA cloning, immunoprecipitation kinase assay, overexpression with downstream MAPK pathway reporter assays Oncogene Medium 9160885
2012 PDCD10 (CCM3) physically interacts with STK25, and co-expression of both proteins accelerates cell apoptosis under oxidative stress (H2O2). PDCD10 stabilizes STK25 protein through a proteasome-dependent pathway. PDCD10/STK25 interaction modulates ERK activity under oxidative stress. Co-immunoprecipitation, siRNA knockdown, overexpression, apoptosis assays, proteasome inhibitor experiments, ERK activity measurement Frontiers in bioscience (Landmark edition) Medium 22652780
2012 STK25 physically interacts with CCM2 (cerebral cavernous malformation 2 protein) and can phosphorylate CCM2. STK25 is part of the TrkA-CCM2 death-signaling pathway in medulloblastoma cells: knockdown of STK25 (but not STK24) rescues cells from NGF-induced TrkA-dependent cell death, and the kinase activity of STK25 is required for death signaling. Affinity proteomics (AP-MS), co-immunoprecipitation, in vitro kinase assay, siRNA knockdown, cell death assays, kinase-dead mutant The Journal of biological chemistry High 22782892
2012 Stk25 knockdown in embryonic neurons reduces Tau phosphorylation. Stk25 regulates neuronal polarization and Golgi morphology in an antagonistic manner to Dab1 (Reelin pathway), as identified by microarray-based modifier screen in dab1-null mice and validated by knockdown. Microarray gene expression comparison between strains, siRNA knockdown in embryonic neurons, Tau phosphorylation assay, Golgi morphology imaging PloS one Medium 22355340
2013 Partial depletion of STK25 in rat L6 myoblasts increases expression of Ucp3 and lipid oxidation, and enhances expression of Glut1, Glut4, and hexokinase 2, resulting in improved insulin-stimulated glucose uptake. STK25 thus negatively regulates lipid oxidation and glucose uptake in skeletal muscle cells. siRNA knockdown in L6 myoblasts, qRT-PCR, western blot, palmitate oxidation assay, glucose uptake assay Diabetologia Medium 22391949
2013 Acute loss of Stk25 function (Cre-mediated conditional knockout or siRNA knockdown) disrupts neuronal migration in the developing cortex. Knockdown of LKB1, STRAD, and GM130 — molecules in the LKB1-STRAD-Stk25-GM130 pathway — causes similar neuronal migration errors. Conditional (Cre-mediated) knockout, siRNA knockdown, in utero electroporation, neuronal migration assays in developing mouse brain Neural development Medium 24225308
2015 STK25 localizes to intrahepatic lipid droplets, colocalizing with the lipid droplet-coating protein ADFP/ATGL. STK25 overexpression reduces β-oxidation and triacylglycerol secretion in liver, promoting steatosis, while ATGL is displaced from the lipid droplet surface to the cytoplasm in STK25 transgenic livers. Transgenic mouse model (high-fat diet challenge), immunofluorescence colocalization, subcellular fractionation, β-oxidation assay, TAG secretion assay, western blot FASEB journal Medium 25609431
2015 STK25 deficiency in knockout mice suppresses development of hyperglycemia, reduces hepatic gluconeogenesis, and increases insulin sensitivity. Stk25-/- mice show decreased protein levels of acetyl-CoA carboxylase (ACC), implicating ACC regulation as a mechanism underlying altered lipid oxidation and synthesis. Stk25 knockout mice on high-fat diet, glucose/insulin tolerance tests, euglycemic-hyperinsulinemic clamp, hepatic gene/protein expression analysis, western blot Diabetes Medium 25845663
2019 STK25 activates LATS kinase by promoting LATS activation loop phosphorylation independently of prior hydrophobic motif phosphorylation, thereby activating Hippo signaling and suppressing YAP/TAZ transcriptional co-activators. Loss of STK25 promotes YAP/TAZ activation and enhanced cellular proliferation under growth-suppressive conditions. siRNA/CRISPR loss-of-function, phospho-specific antibody assays for LATS activation loop vs. hydrophobic motif, YAP/TAZ reporter assays, in vitro and in vivo proliferation assays, cancer genomics analysis Nature communications High 30948712
2020 STK25 promotes STRIPAK-mediated inhibition of MST2 (Hippo pathway) by directly phosphorylating the scaffolding protein SAV1, which diminishes SAV1's ability to inhibit the STRIPAK phosphatase complex. Thus STK25, as the kinase component of STRIPAK, antagonizes SAV1 to suppress Hippo signaling initiation. siRNA depletion of STK25 in human cells, in vitro kinase assay (STK25 phosphorylating SAV1), co-immunoprecipitation assessing STRIPAK integrity, MST2 activation assays eLife High 32292165
2018 STK25 interacts with GOLPH3 (Golgi phosphoprotein 3) and suppresses aerobic glycolysis in colorectal cancer cells through GOLPH3-regulated mTOR signaling, thereby inhibiting cell proliferation. Co-immunoprecipitation, GST pull-down, His-tag pull-down, western blot for mTOR pathway, glucose uptake and lactate production assays, xenograft mouse model Journal of experimental & clinical cancer research Medium 29996891
2021 STK25 regulates neuronal migration and polarization by controlling Rho GTPase activities: STK25 promotes Rac1 activation and reduces RhoA levels in the developing brain through forming complexes with α-PIX and β-PIX (GTPase regulatory enzymes) and with Cullin3-Bacurd1/Kctd13 (a RhoA ubiquitination complex), in a kinase activity-independent manner. Stk25 conditional knockout, in utero electroporation, rescue experiments with MST3 overexpression, co-immunoprecipitation for complex formation, RhoA/Rac1 activity assays, overexpression of Bacurd1/Cul3 The Journal of neuroscience Medium 34518307
2022 STK25 phosphorylates the type Iα regulatory subunit of PKA (PRKAR1A), which leads to inhibition of PKA catalytic activity and increased binding of the regulatory subunit to the catalytic subunit in the presence of cAMP. In Stk25 knockout mice, PRKAR1A phosphorylation is diminished, PKA activity is increased, and contractile response to beta-adrenergic stimulation is augmented. iPSC-derived cardiomyocytes, in vitro kinase assay, co-immunoprecipitation (regulatory-catalytic subunit binding), Stk25 knockout mouse model, PKA activity assay, cardiac contractility measurements Cell reports High 35977512
2022 STK25 deficiency in mice and human endothelial cells causes KLF2 expression, Golgi dispersion, altered β-catenin distribution, and stress fiber appearance. Combined deficiency of STK24 and STK25 (but not either alone) in mice causes aggressive cavernoma-like vascular lesions, and STK25 deficiency alone induces these phenotypes in the context of STK24 heterozygosity. Double Stk24/Stk25 knockout mice, siRNA in human endothelial cells, histology, immunofluorescence for KLF2/β-catenin/stress fibers/Golgi morphology Stroke Medium 35130716
2022 STK25 deficiency increases PD-L1 protein stability by regulating K48-linked ubiquitination of PD-L1 in a NEDD4-dependent manner, thereby increasing PD-L1 surface levels and modulating tumor immune evasion in colorectal cancer. STK25 knockout cell lines and mice, co-immunoprecipitation, ubiquitination assays (K48-linkage specific), western blot for PD-L1 protein levels, in vivo tumor models with anti-PD-1 treatment Advanced science Medium 40729594
2025 STK25 undergoes autophosphorylation in response to multiple TLR triggers and phosphorylates IRF5 at Thr265, leading to IRF5 transcriptional activation and pro-inflammatory cytokine production. Loss of STK25 in primary immune cells attenuates R848-induced IRF5 nuclear translocation and pro-inflammatory cytokine production. Kinome-wide siRNA screen in THP-1 cells, in vitro kinase assay (STK25 phosphorylating IRF5-Thr265), Stk25-deficient primary immune cells, IRF5 nuclear translocation assay, cytokine ELISA Life science alliance Medium 40639948
2019 STK25 coats intrahepatic lipid droplets and regulates their associated phosphoproteome; STK25 deficiency in the liver alters proteins involved in peroxisomal biogenesis, ubiquitination-mediated proteolysis, and antioxidant defense. STK25 silencing in human liver cells attenuates peroxisomal biogenesis and protects against oxidative and ER stress in a hepatocyte-autonomous manner. Stk25 knockout mice (high-fat diet), quantitative lipid droplet-associated phosphoproteomics, peroxisomal biogenesis assays, oxidative/ER stress markers in human liver cells with STK25 siRNA Journal of lipid research Medium 31857389
2026 STK25 deficiency in colorectal cancer cells activates the NF-κB pathway, leading to p50 phosphorylation that directly binds the AREG promoter and transcriptionally upregulates AREG expression; elevated AREG then activates EGFR on cancer-associated fibroblasts (CAFs), promoting CAF activation and cetuximab resistance. siRNA knockdown and overexpression in CRC cells, ChIP assay (p50 binding to AREG promoter), dual-luciferase reporter assay, CAF co-culture, in vivo xenograft models, patient-derived organoids Clinical and translational medicine Medium 42057436

Source papers

Stage 0 corpus · 48 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 YSK1 is activated by the Golgi matrix protein GM130 and plays a role in cell migration through its substrate 14-3-3zeta. The Journal of cell biology 229 15037601
2010 Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 137 21111240
2011 MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases. The EMBO journal 122 21423148
2015 Protein kinase STK25 regulates hepatic lipid partitioning and progression of liver steatosis and NASH. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 71 25609431
2015 Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model. Diabetes 53 25845663
2016 STK25 is a critical determinant in nonalcoholic steatohepatitis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 46 27421788
2015 Protein kinase STK25 controls lipid partitioning in hepatocytes and correlates with liver fat content in humans. Diabetologia 46 26553096
2008 SOK1 translocates from the Golgi to the nucleus upon chemical anoxia and induces apoptotic cell death. The Journal of biological chemistry 45 18364353
1994 Suppression of a yeast cyclic AMP-dependent protein kinase defect by overexpression of SOK1, a yeast gene exhibiting sequence similarity to a developmentally regulated mouse gene. Molecular and cellular biology 45 8065298
2013 Increased expression of STK25 leads to impaired glucose utilization and insulin sensitivity in mice challenged with a high-fat diet. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 44 23729594
2018 Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease. Cellular and molecular gastroenterology and hepatology 43 30576769
2019 Identification of the kinase STK25 as an upstream activator of LATS signaling. Nature communications 41 30948712
2020 STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism. eLife 39 32292165
2016 Overexpression of protein kinase STK25 in mice exacerbates ectopic lipid accumulation, mitochondrial dysfunction and insulin resistance in skeletal muscle. Diabetologia 38 27981357
2012 Serine/threonine protein kinase 25 (STK25): a novel negative regulator of lipid and glucose metabolism in rodent and human skeletal muscle. Diabetologia 38 22391949
2013 Acute inactivation of the serine-threonine kinase Stk25 disrupts neuronal migration. Neural development 36 24225308
2018 STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer. Journal of experimental & clinical cancer research : CR 32 29996891
2012 PDCD10 interacts with STK25 to accelerate cell apoptosis under oxidative stress. Frontiers in bioscience (Landmark edition) 30 22652780
1997 YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways. Oncogene 30 9160885
2019 Lipid droplet-associated kinase STK25 regulates peroxisomal activity and metabolic stress response in steatotic liver. Journal of lipid research 26 31857389
2017 Protein kinase STK25 aggravates the severity of non-alcoholic fatty pancreas disease in mice. The Journal of endocrinology 24 28442507
2014 Differential expression of MST4, STK25 and PDCD10 between benign prostatic hyperplasia and prostate cancer. International journal of clinical and experimental pathology 24 25550858
2012 STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor cells of neural origin. The Journal of biological chemistry 20 22782892
2022 STK25 enhances hepatocellular carcinoma progression through the STRN/AMPK/ACC1 pathway. Cancer cell international 19 34986838
2020 Depletion of protein kinase STK25 ameliorates renal lipotoxicity and protects against diabetic kidney disease. JCI insight 16 33170807
2018 STK25 regulates oxidative capacity and metabolic efficiency in adipose tissue. The Journal of endocrinology 16 29794231
2022 Exosomal miR-4800-3p Aggravates the Progression of Hepatocellular Carcinoma via Regulating the Hippo Signaling Pathway by Targeting STK25. Frontiers in oncology 15 35756606
2021 Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways. Cellular and molecular gastroenterology and hepatology 15 34624527
2018 STK25 Regulates Cardiovascular Disease Progression in a Mouse Model of Hypercholesterolemia. Arteriosclerosis, thrombosis, and vascular biology 15 29930001
2012 Identification of Stk25 as a genetic modifier of Tau phosphorylation in Dab1-mutant mice. PloS one 15 22355340
2011 Downregulation of SOK1 promotes the migration of MCF-7 cells. Biochemical and biophysical research communications 15 21396913
2001 STK25 is a candidate gene for pseudopseudohypoparathyroidism. Genomics 15 11543625
2008 Farp2 and Stk25 are candidate genes for the HDL cholesterol locus on mouse chromosome 1. Arteriosclerosis, thrombosis, and vascular biology 14 18988887
2022 LIMK1 Interacts with STK25 to Regulate EMT and Promote the Proliferation and Metastasis of Colorectal Cancer. Journal of oncology 13 35265128
2022 Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer. Molecular carcinogenesis 13 35349179
2021 STK25 and MST3 Have Overlapping Roles to Regulate Rho GTPases during Cortical Development. The Journal of neuroscience : the official journal of the Society for Neuroscience 12 34518307
2022 STK25 inhibits PKA signaling by phosphorylating PRKAR1A. Cell reports 10 35977512
2015 A novel 2q37 microdeletion containing human neural progenitors genes including STK25 results in severe developmental delay, epilepsy, and microcephaly. American journal of medical genetics. Part A 9 26238961
2022 GCKIII (Germinal Center Kinase III) Kinases STK24 and STK25 (Serine/Threonine Kinase 24 and 25) Inhibit Cavernoma Development. Stroke 8 35130716
2022 Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective. Communications biology 7 35440683
2022 Activation of YAP1 by STK25 contributes to the progression of hepatocellular carcinoma. Tissue & cell 5 35421669
2022 Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways. Cellular and molecular neurobiology 4 35661286
2025 STK25 Loss Augments Anti-PD-1 Therapy Efficacy by Regulating PD-L1 Stability in Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 3 40729594
2025 Therapeutic Potential of STE20-Type Kinase STK25 Inhibition for the Prevention and Treatment of Metabolically Induced Hepatocellular Carcinoma. Cellular and molecular gastroenterology and hepatology 2 40024534
2025 Inhibition of GCKIII kinases STK25 and MST3 mitigates organ lipotoxicity and enhances metabolic resilience under nutritional stress. BMC medicine 2 40983918
2025 TLR-induced STK25 activation promotes IRF5-mediated inflammation. Life science alliance 1 40639948
2023 STK25: a viable therapeutic target for cancer treatments? Anti-cancer drugs 1 36728989
2026 STK25 inhibits cancer-associated fibroblast activation to overcome cetuximab resistance in colorectal cancer. Clinical and translational medicine 0 42057436

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