SOWAHC

SOWAHC (C2orf26/ANKRD57) is a protein of essentially uncharacterized function. Its mouse ortholog, designated TSC77, localizes to the nucleus when expressed as a GFP-fusion in Cos-7 cells and carries computationally predicted NirB-, HcaD-, and Ndh-related (NAD/FAD-dependent oxidoreductase) domains (PMID:17309138). Beyond this single localization observation and the computational domain prediction, no enzymatic activity, binding partners, or pathway placement have been experimentally established in the available corpus.

1. 2007 Low

   The first question was where the protein resides and what domains it might contain, addressed by characterizing the mouse ortholog TSC77, which established a nuclear localization and flagged candidate oxidoreductase-related sequence features.

   Evidence GFP-fusion subcellular localization imaging in Cos-7 cells with bioinformatics domain prediction and RT-PCR expression analysis

   PMID:17309138

   Open questions at the time

   • Single lab using a single overexpressed GFP-fusion method without endogenous protein validation
   • Predicted NirB/HcaD/Ndh domains are computational only, with no enzymatic assay confirming oxidoreductase activity
   • No binding partners, substrates, or pathway context identified

• Whether SOWAHC has any catalytic activity, what molecules or proteins it interacts with, and what cellular process it participates in remain entirely unresolved.
• No direct enzymatic activity demonstrated for the predicted oxidoreductase domains
• No physical interaction partners identified
• No upstream/downstream pathway placement established