{"gene":"SOWAHC","run_date":"2026-06-10T07:46:38","timeline":{"discoveries":[{"year":2007,"finding":"The mouse ortholog of SOWAHC (C2orf26/ANKRD57), designated TSC77, localizes to the nucleus of Cos-7 cells when expressed as a GFP-fusion protein, and contains predicted NirB, HcaD, and Ndh domains in its protein sequence.","method":"GFP-fusion subcellular localization imaging in Cos-7 cells; bioinformatics domain prediction; RT-PCR expression analysis","journal":"Croatian medical journal","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single localization method (GFP fusion) with no functional follow-up; domain predictions are computational only","pmids":["17309138"],"is_preprint":false}],"current_model":"SOWAHC (C2orf26/ANKRD57) encodes a protein whose mouse ortholog (TSC77) localizes to the nucleus and contains predicted NAD/FAD-dependent oxidoreductase-related domains, but no direct enzymatic activity, binding partners, or upstream/downstream pathway placement have been experimentally established for this protein."},"narrative":{"mechanistic_narrative":"SOWAHC (C2orf26/ANKRD57) is a protein of essentially uncharacterized function. Its mouse ortholog, designated TSC77, localizes to the nucleus when expressed as a GFP-fusion in Cos-7 cells and carries computationally predicted NirB-, HcaD-, and Ndh-related (NAD/FAD-dependent oxidoreductase) domains [PMID:17309138]. Beyond this single localization observation and the computational domain prediction, no enzymatic activity, binding partners, or pathway placement have been experimentally established in the available corpus.","teleology":[{"year":2007,"claim":"The first question was where the protein resides and what domains it might contain, addressed by characterizing the mouse ortholog TSC77, which established a nuclear localization and flagged candidate oxidoreductase-related sequence features.","evidence":"GFP-fusion subcellular localization imaging in Cos-7 cells with bioinformatics domain prediction and RT-PCR expression analysis","pmids":["17309138"],"confidence":"Low","gaps":["Single lab using a single overexpressed GFP-fusion method without endogenous protein validation","Predicted NirB/HcaD/Ndh domains are computational only, with no enzymatic assay confirming oxidoreductase activity","No binding partners, substrates, or pathway context identified"]},{"year":null,"claim":"Whether SOWAHC has any catalytic activity, what molecules or proteins it interacts with, and what cellular process it participates in remain entirely unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No direct enzymatic activity demonstrated for the predicted oxidoreductase domains","No physical interaction partners identified","No upstream/downstream pathway placement established"]}],"mechanism_profile":{"molecular_activity":[],"localization":[{"term_id":"GO:0005634","term_label":"nucleus","supporting_discovery_ids":[0]}],"pathway":[],"complexes":[],"partners":[],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q53LP3","full_name":"Ankyrin repeat domain-containing protein SOWAHC","aliases":["Ankyrin repeat domain-containing protein 57","Protein sosondowah homolog C"],"length_aa":525,"mass_kda":55.7,"function":"","subcellular_location":"","url":"https://www.uniprot.org/uniprotkb/Q53LP3/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/SOWAHC","classification":"Not Classified","n_dependent_lines":126,"n_total_lines":1208,"dependency_fraction":0.10430463576158941},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"CAPZB","stoichiometry":0.2},{"gene":"DIAPH1","stoichiometry":0.2},{"gene":"PFN1","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/SOWAHC","total_profiled":1310},"omim":[],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Cytosol","reliability":"Approved"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in many","driving_tissues":[],"url":"https://www.proteinatlas.org/search/SOWAHC"},"hgnc":{"alias_symbol":["FLJ21870"],"prev_symbol":["C2orf26","ANKRD57"]},"alphafold":{"accession":"Q53LP3","domains":[{"cath_id":"1.10.10","chopping":"16-84","consensus_level":"high","plddt":87.6081,"start":16,"end":84},{"cath_id":"1.25.40.20","chopping":"267-395","consensus_level":"high","plddt":90.9774,"start":267,"end":395}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q53LP3","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q53LP3-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q53LP3-F1-predicted_aligned_error_v6.png","plddt_mean":60.25},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=SOWAHC","jax_strain_url":"https://www.jax.org/strain/search?query=SOWAHC"},"sequence":{"accession":"Q53LP3","fasta_url":"https://rest.uniprot.org/uniprotkb/Q53LP3.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q53LP3/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q53LP3"}},"corpus_meta":[{"pmid":"20224928","id":"PMC_20224928","title":"Identification of genes associated with chemosensitivity to SAHA/taxane combination treatment in taxane-resistant breast cancer cells.","date":"2010","source":"Breast cancer research and treatment","url":"https://pubmed.ncbi.nlm.nih.gov/20224928","citation_count":48,"is_preprint":false},{"pmid":"17309138","id":"PMC_17309138","title":"Identification of a novel testis-specific gene in mice and its potential roles in spermatogenesis.","date":"2007","source":"Croatian medical journal","url":"https://pubmed.ncbi.nlm.nih.gov/17309138","citation_count":5,"is_preprint":false},{"pmid":"40876114","id":"PMC_40876114","title":"Genome-wide association study identifying immune markers in Qingyuan partridge chickens infected with avian Leukosis virus subgroup J.","date":"2025","source":"Poultry science","url":"https://pubmed.ncbi.nlm.nih.gov/40876114","citation_count":1,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3774,"output_tokens":318,"usd":0.008046,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6548,"output_tokens":800,"usd":0.02637,"stage2_stop_reason":"end_turn"},"total_usd":0.034416,"stage1_batch_id":"msgbatch_013LWZZT6MWaqB9qyTSeQBmU","stage2_batch_id":"msgbatch_0194ZzQaTGNpruoNJBbEMbt3","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2007,\n      \"finding\": \"The mouse ortholog of SOWAHC (C2orf26/ANKRD57), designated TSC77, localizes to the nucleus of Cos-7 cells when expressed as a GFP-fusion protein, and contains predicted NirB, HcaD, and Ndh domains in its protein sequence.\",\n      \"method\": \"GFP-fusion subcellular localization imaging in Cos-7 cells; bioinformatics domain prediction; RT-PCR expression analysis\",\n      \"journal\": \"Croatian medical journal\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single localization method (GFP fusion) with no functional follow-up; domain predictions are computational only\",\n      \"pmids\": [\"17309138\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"SOWAHC (C2orf26/ANKRD57) encodes a protein whose mouse ortholog (TSC77) localizes to the nucleus and contains predicted NAD/FAD-dependent oxidoreductase-related domains, but no direct enzymatic activity, binding partners, or upstream/downstream pathway placement have been experimentally established for this protein.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"SOWAHC (C2orf26/ANKRD57) is a protein of essentially uncharacterized function. Its mouse ortholog, designated TSC77, localizes to the nucleus when expressed as a GFP-fusion in Cos-7 cells and carries computationally predicted NirB-, HcaD-, and Ndh-related (NAD/FAD-dependent oxidoreductase) domains [#0]. Beyond this single localization observation and the computational domain prediction, no enzymatic activity, binding partners, or pathway placement have been experimentally established in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2007,\n      \"claim\": \"The first question was where the protein resides and what domains it might contain, addressed by characterizing the mouse ortholog TSC77, which established a nuclear localization and flagged candidate oxidoreductase-related sequence features.\",\n      \"evidence\": \"GFP-fusion subcellular localization imaging in Cos-7 cells with bioinformatics domain prediction and RT-PCR expression analysis\",\n      \"pmids\": [\"17309138\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single lab using a single overexpressed GFP-fusion method without endogenous protein validation\",\n        \"Predicted NirB/HcaD/Ndh domains are computational only, with no enzymatic assay confirming oxidoreductase activity\",\n        \"No binding partners, substrates, or pathway context identified\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether SOWAHC has any catalytic activity, what molecules or proteins it interacts with, and what cellular process it participates in remain entirely unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct enzymatic activity demonstrated for the predicted oxidoreductase domains\",\n        \"No physical interaction partners identified\",\n        \"No upstream/downstream pathway placement established\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"faith_supported":1,"faith_total":1,"faith_pct":100.0}}