Affinage

DIAPH1

Protein diaphanous homolog 1 · UniProt O60610

Length
1272 aa
Mass
141.3 kDa
Annotated
2026-04-28
100 papers in source corpus 58 papers cited in narrative 58 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DIAPH1 (mDia1) is a Rho-family GTPase effector formin that nucleates and processively elongates unbranched actin filaments while simultaneously coordinating microtubule stabilization, thereby integrating cytoskeletal dynamics with mechanosensing, cell migration, immune cell function, and intracellular signaling. The protein is maintained in an autoinhibited state by an intramolecular DID–DAD interaction; RhoA-GTP binding to the DID partially displaces the DAD, releasing the dimeric FH2 domain to processively cap and elongate barbed ends—rotating along the actin helix to generate cofilin-resistant filaments—while the FH1 domain recruits profilin–actin monomers, with activity further tuned by Cdk1 phosphorylation, G-actin feedback, and partners including CLIP-170, APC, SPIN90–Arp2/3, Flightless-I, IRSp53, and IQGAP1–INF2 (PMID:12906795, PMID:14657240, PMID:15866170, PMID:20927338, PMID:21148346, PMID:29760064, PMID:30816115, PMID:32572169). In vivo, DIAPH1 is required for T-cell chemotaxis, dendritic cell migration, NK cell cytotoxicity, megakaryocyte proplatelet formation, spermatogenesis, and RAGE-dependent signaling that drives diabetic complications and atherosclerosis (PMID:17682067, PMID:20881208, PMID:19913427, PMID:25298036, PMID:30256801, PMID:34818060, PMID:36932214). Truncating mutations in the DAD domain cause constitutive activation leading to macrothrombocytopenia and progressive sensorineural hearing loss (DFNA1), while complete loss-of-function produces myeloproliferative disease and combined immune deficiency (PMID:9360932, PMID:26912466, PMID:27707755, PMID:17699759, PMID:33662367).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1997 High

    Identification of DIAPH1 as a Rho-targeted profilin ligand and its linkage to autosomal dominant deafness DFNA1 established the gene as a cytoskeletal regulator with clinical relevance.

    Evidence Genetic linkage in a large kindred with sensorineural hearing loss; splice-donor mutation identified

    PMID:9360932

    Open questions at the time
    • Biochemical activity of the protein was not yet demonstrated
    • Mechanism of hearing loss was undefined
  2. 1999 High

    Demonstration that RhoA-GTP relieves mDia1 autoinhibition to induce thin actin stress fibers—cooperating with ROCK for mature fibers—placed mDia1 as a bifurcating Rho effector distinct from the ROCK–myosin pathway.

    Evidence Constitutively active and dominant-negative mDia1 mutants in cells; co-expression with ROCK inhibitors; fluorescence microscopy

    PMID:10559899

    Open questions at the time
    • The molecular basis of autoinhibition was unknown
    • Direct actin nucleation activity was not yet shown in vitro
  3. 2001 High

    A series of studies established that mDia1 coordinates both actin and microtubule organization, mediates force-dependent focal adhesion maturation independently of ROCK, and localizes to the mitotic spindle, broadening its role beyond simple actin assembly.

    Evidence Micropipette force application with constitutively active mDia1 rescue; FH1/FH2 domain mutants in HeLa cells; immunocytochemistry of mitotic spindle with GFP-mDia1 truncations

    PMID:11146620 PMID:11171383 PMID:11402062

    Open questions at the time
    • How FH2 aligns microtubules was mechanistically unclear
    • Whether spindle localization is functionally required was untested
  4. 2003 High

    Reconstitution of mDia1 FH2 as a potent actin nucleator that processively associates with barbed ends—protected from capping protein—defined the core formin mechanism and showed RhoA only partially relieves N-terminal autoinhibition in vitro.

    Evidence In vitro pyrene-actin polymerization; TIRF microscopy; capping protein competition; cross-species complementation in yeast

    PMID:12906795 PMID:14657240

    Open questions at the time
    • Full structural basis of autoinhibition remained unsolved
    • Additional activation inputs beyond RhoA were suspected but undefined
  5. 2005 High

    Crystal structure of the mDia1 N-terminal regulatory region revealed the DID as an armadillo-repeat module, with NMR mapping showing overlapping RhoA and DAD binding sites, explaining how GTPase binding competitively displaces the DAD to relieve autoinhibition.

    Evidence X-ray crystallography; NMR chemical-shift mapping; ITC and pulldown binding assays

    PMID:15866170

    Open questions at the time
    • Structure of the full-length autoinhibited complex was lacking
    • How partial relief of autoinhibition translates to graded activity in cells was unknown
  6. 2007 High

    Knockout mice revealed essential in vivo roles: mDia1 loss causes T-cell lymphopenia with impaired chemotaxis and age-dependent myeloproliferative disease, establishing DIAPH1 as critical for immune cell function and hematopoietic homeostasis.

    Evidence Drf1 knockout mice; chemotaxis and adhesion assays; flow cytometry; hematopoietic phenotyping and histology

    PMID:17682067 PMID:17699759

    Open questions at the time
    • Molecular mechanism linking mDia1 loss to myeloproliferation was unclear
    • Whether the immune phenotype is purely cytoskeletal or involves signaling crosstalk was unresolved
  7. 2010 High

    Multiple discoveries in 2010 extended mDia1 biology: helical rotation during processive elongation was directly visualized; the ctRAGE–mDia1 interaction was structurally defined as essential for RAGE signaling; Flightless-I was shown to enhance mDia1 activity; APC was found to synergize with mDia1 for actin nucleation; and mDia1 was linked to mitochondrial trafficking and steroidogenesis.

    Evidence Single-molecule fluorescence polarization (rotation); NMR of ctRAGE with mutagenesis; in vitro reconstitution of Fli-I and APC effects; live-cell mitochondrial imaging with siRNA

    PMID:20223827 PMID:20566685 PMID:20591975 PMID:21148346 PMID:22194616

    Open questions at the time
    • How helical rotation affects filament properties in cells was unexplored
    • Whether ctRAGE binding alters mDia1 formin activity was untested
  8. 2012 High

    A capping-protein-dependent mechanism was identified in which competition at barbed ends releases mDia1 to stabilize detyrosinated microtubules, and mDia1 was shown to be required for RAGE-ligand-induced vascular smooth muscle migration and neointimal expansion.

    Evidence siRNA of capping protein and mDia1 with immunofluorescence of Glu-MTs; mDia1 KO mice with femoral artery injury; Rac1-GTP and AKT phosphorylation assays

    PMID:22511750 PMID:22918941

    Open questions at the time
    • The structural basis for mDia1-microtubule interaction was incomplete
    • Relative contributions of actin vs. microtubule functions of mDia1 in vascular disease were not dissected
  9. 2016 High

    Gain-of-function DIAPH1 DAD-truncating mutations (R1213*, R1204X) were shown to constitutively activate the protein by disrupting DID–DAD interaction, causing macrothrombocytopenia and progressive deafness with stereocilia defects in patients and transgenic mice, linking autoinhibition relief directly to disease.

    Evidence Exome sequencing; DID-DAD binding assays; single-molecule actin polymerization rates; transgenic mouse ABR and SEM of stereocilia

    PMID:26912466 PMID:27707755

    Open questions at the time
    • How constitutive activation disrupts megakaryocyte biology at the molecular level was not fully defined
    • Whether hearing loss is reversible was untested
  10. 2016 High

    An mDia1–INF2–IQGAP1 formin cascade was shown to be required for LPA-induced stable microtubule formation, and pharmacological disruption of ctRAGE–DIAPH1 interaction validated this interface as a druggable target for RAGE signaling.

    Evidence siRNA epistasis with GST pulldown of IQGAP1–INF2; HTS of 58,000 compounds with NMR competition and in vivo pharmacology

    PMID:26936329 PMID:27030671

    Open questions at the time
    • How the formin cascade is spatiotemporally organized in cells was unclear
    • In vivo efficacy of ctRAGE–DIAPH1 inhibitors in chronic disease models had limited data
  11. 2018 High

    Helical rotation during elongation was shown to untwist F-actin, generating cofilin-resistant filaments, and mDia1/3 double KO revealed essential roles in Sertoli cell cortical actin and spermatogenesis, while mDia1 was found to promote TGFβRII endocytosis via Rab5a interaction.

    Evidence Single-molecule polarization and EM of actin twist; mDia1/3 DKO mice with superresolution imaging; co-IP of Diaph1–TβRII–Rab5a with active Rab5a mutants

    PMID:29760064 PMID:30256801 PMID:32304339

    Open questions at the time
    • Whether cofilin resistance is the dominant mechanism for stress fiber stability in vivo was unclear
    • Rab5a activation mechanism by Diaph1 was not structurally characterized
  12. 2019 High

    Cdk1 phosphorylation of DIAPH1 was shown to block profilin1 binding, regulating cortical tension in mitosis and enabling proper kinetochore stretching and spindle assembly checkpoint silencing, establishing a mitotic regulatory input.

    Evidence Phosphosite mutants; profilin1 binding assay; cortical tension measurement; intra-kinetochore distance; live-cell imaging

    PMID:30816115

    Open questions at the time
    • Identity of the phosphatase reversing this modification was unknown
    • Whether other formins are similarly regulated by Cdk1 was untested
  13. 2020 High

    SPIN90 was discovered to form a ternary complex with Arp2/3 and mDia1, recruiting mDia1 to Arp2/3-nucleated pointed ends to produce rapidly elongating unbranched filaments, redefining how branched and linear nucleation pathways cooperate.

    Evidence In vitro reconstitution with TIRF microscopy; biochemical pulldown; cellular actin network analysis

    PMID:32572169

    Open questions at the time
    • Whether SPIN90 regulation is cell-type-specific was unknown
    • Structural basis of the ternary complex was not determined
  14. 2021 High

    DIAPH1 loss was linked to mitochondrial complex IV dysfunction and combined immune deficiency, ctRAGE–DIAPH1 small-molecule antagonists were validated in diabetic mouse models, and tension-controlled pulsatile actin polymerization at focal adhesions was shown to depend on mDia1.

    Evidence CRISPR KO in PBMCs with mitochondrial activity assays; NMR-guided pharmacology in STZ-diabetic mice; traction force microscopy with mDia1 KD

    PMID:33662367 PMID:34818060 PMID:34822787

    Open questions at the time
    • Mechanism linking mDia1 to complex IV function is unknown
    • Whether RAGE229 has efficacy in human disease was untested
    • How mechanical feedback tunes mDia1 activation at focal adhesions was not resolved
  15. 2023 High

    DIAPH1 was shown to promote SREBP1 nuclear translocation via the actin cytoskeleton, connecting it to hepatic lipid metabolism and atherosclerosis progression independently of canonical metabolic signaling.

    Evidence Ldlr−/− Diaph1−/− double-KO mice on Western diet; hepatic SREBP1 nuclear translocation; lipid measurements

    PMID:36932214

    Open questions at the time
    • How actin-dependent SREBP1 translocation is mechanistically achieved was not defined
    • Whether DIAPH1 formin activity or a scaffolding function drives this effect was not distinguished

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of the full-length autoinhibited dimer, how DIAPH1 partitions between actin and microtubule substrates in real time, the mechanism by which DIAPH1 controls mitochondrial complex IV activity, and whether therapeutic targeting of ctRAGE–DIAPH1 is efficacious in human metabolic and inflammatory disease.
  • No full-length autoinhibited structure exists
  • Actin vs. microtubule substrate partitioning is not mechanistically resolved
  • Mitochondrial complex IV link is correlative without defined molecular mechanism

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 7 GO:0098772 molecular function regulator activity 5 GO:0140096 catalytic activity, acting on a protein 5
Localization
GO:0005856 cytoskeleton 7 GO:0005886 plasma membrane 4 GO:0005794 Golgi apparatus 2 GO:0005815 microtubule organizing center 2 GO:0005829 cytosol 2
Pathway
R-HSA-162582 Signal Transduction 7 R-HSA-168256 Immune System 4 R-HSA-109582 Hemostasis 3 R-HSA-1500931 Cell-Cell communication 2 R-HSA-1640170 Cell Cycle 2 R-HSA-1430728 Metabolism 1
Partners
AGERAPCCLIP170FLIIINF2IQGAP1IRSP53RHOA
Complex memberships
SPIN90-Arp2/3-mDia1 ternary complexmDia1-INF2-IQGAP1 formin cascade

Evidence

Reading pass · 58 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 DIAPH1 (human diaphanous homolog) was identified as a profilin ligand and target of Rho GTPase that regulates polymerization of actin; a protein-truncating splice-donor mutation causes autosomal dominant sensorineural hearing loss DFNA1. Genetic linkage mapping + mutation identification in a large kindred; functional annotation as profilin ligand and Rho target Science High 9360932
1999 GTP-bound RhoA activates mDia1 by disrupting its intramolecular autoinhibitory interaction; active mDia1 induces formation of thin actin stress fibers and cooperates with ROCK to produce mature stress fibers. Constitutively active and dominant-negative mutant expression in cells; co-expression with ROCK inhibitors; fluorescence microscopy of actin structures Nature cell biology High 10559899
2001 mDia1 mediates force-induced focal contact formation downstream of Rho, independently of ROCK-mediated myosin II contractility; integrin-containing focal complexes act as mechanosensors that respond to externally applied tension via mDia1. Micropipette force application to cells; C3 transferase Rho inhibition; constitutively active mDia1 rescue; GFP-vinculin/paxillin live imaging; ROCK and myosin II inhibitors The Journal of cell biology High 11402062
2001 Active mDia1 (via its FH1 and FH2 regions) coordinates microtubule alignment parallel to F-actin bundles; the FH2 region is required for microtubule alignment and cell elongation, whereas FH1 mediates actin effects. Expression of mDia1 deletion and point mutants (FH1/FH2) in HeLa cells; immunofluorescence of microtubules and F-actin Nature cell biology High 11146620
2001 mDia1 localizes to the mitotic spindle from prophase to telophase in HeLa cells; spindle localization is determined by a 173-amino-acid sequence in the FH3 region (Leu434 and Leu455 required) and is independent of Rho activity. Immunocytochemistry with anti-mDia1 antibody using instantaneous fixation; GFP-mDia1 truncation mutants; mitotic spindle fractionation + Western blot; microinjection of C3/Val14RhoA Journal of cell science High 11171383
2001 The FH1 domain of mDia1 binds profilin in vitro and in cells; the RBD (Rho-binding domain) complexes with the C-terminal CIID autoinhibitory module; overexpression of the RBD alone causes spontaneous ruffling, loss of stress fibers, and upregulation of Rac activity. In vitro binding assays; transfection of deletion constructs; dominant-negative Rac co-expression; fluorescence microscopy Journal of cell science Medium 11707518
2002 mDia1 mediates Rho-dependent Rac activation through a Cas phosphorylation/Crk-II/DOCK180 pathway that is antagonized by ROCK; active mDia1 dominant-negative inhibits membrane ruffle formation induced by ROCK inhibition. ROCK inhibitor (Y-27632) vs. C3 exoenzyme comparison; dominant-negative mDia1; Rac-GTP pull-down assay; phosphotyrosine analysis; dominant-negative Cas/Crk mutants The Journal of cell biology High 12021256
2002 mDia1 activates serum response factor (SRF) through actin polymerization; the FH2 domain (and extended C-terminal region) is required for both F-actin assembly and SRF activation; actin depletion of the G-actin pool downstream of mDia1 is the signal to SRF. SRF luciferase reporter assay; mDia1 deletion/point mutants; nonpolymerizable actin mutant; dominant-negative mDia1 derivatives blocking serum/LPA-induced SRF Molecular biology of the cell High 12429848
2003 Mouse mDia1 FH2-containing constructs are potent actin nucleators in vitro; the FH1 domain is required for nucleation from profilin-bound actin monomers; the N-terminal GBD strongly inhibits C-terminal actin nucleation activity (autoinhibition model); RhoA partially relieves this inhibition. In vitro pyrene-actin polymerization assay; recombinant mDia1 domain constructs; profilin-actin nucleation assay; gel filtration (multimer analysis) Current biology High 12906795
2003 mDia1 FH2 dimers nucleate and processively associate with actin filament barbed ends, protecting them from capping protein; this mechanism is conserved between yeast Bni1 and mammalian mDia1. In vitro pyrene-actin polymerization; TIRF microscopy; capping protein competition assay; in vivo complementation of bni1 mutant yeast with mDia1 Molecular biology of the cell High 14657240
2003 Genetic disruption of Drf1 (mDia1) reveals that mDia1 loss leads to compensatory upregulation of Drf3 (mDia2), which acts as a Cdc42 effector via a CRIB-like motif in its GBD; Drf3 is recruited by Cdc42 to the leading edge and MTOC during migration. Embryonic stem cell-derived Drf1 knockout cell lines; dominant-negative Drf3 and anti-Drf3 antibody microinjection; FRET analysis of Cdc42-Drf3 binding; fluorescence microscopy Current biology Medium 12676083
2004 mDia1 interacts with PKD2 (polycystin-2) at the mitotic spindle; the interaction is mediated by the PKD2 cytoplasmic C-terminus binding to the mDia1 N-terminus; mDia1 knockdown displaces PKD2 from mitotic spindles and alters intracellular Ca2+ release. Yeast two-hybrid screen; co-immunoprecipitation in native and transfected cells; RNAi knockdown; immunofluorescence co-localization; Ca2+ measurements The Journal of biological chemistry High 15123714
2005 Crystal structure of the dimeric mDia1 regulatory N-terminal domain reveals an intertwined six-helix bundle with two DID modules (five armadillo repeats each); NMR and biochemical mapping show RhoA and DAD binding sites partially overlap on the DID, explaining GTPase-mediated autoinhibition relief. X-ray crystallography; NMR chemical-shift mapping; biochemical binding assays (pulldown/ITC) Molecular cell High 15866170
2006 mDia1 autoinhibition (DID-DAD intramolecular interaction) controls both in vitro actin assembly activity and in vivo membrane localization; Cdc42 relieves FRLα autoinhibition, demonstrating that this is a general DRF regulatory principle. In vitro actin assembly assay; live-cell membrane localization imaging; phagocytosis assay; dominant-negative and constitutively active DRF mutants The Journal of cell biology High 16943183
2006 The Rho-mDia1 pathway regulates directed cell migration by aligning microtubules (delivering APC and active Cdc42 to the cell front for polarity) and actin filaments (recruiting active c-Src to focal adhesions for adhesion turnover). RNAi knockdown of mDia1 in C6 glioma cells; constitutively active mDia1 expression; live fluorescence imaging of APC, Cdc42, c-Src localization; wound healing/migration assays Molecular and cellular biology High 16943426
2006 HAN11 binds the FH2 actin-binding domain of mDia1; overexpression of mDia1 or active RhoA causes translocation of HAN11 from nucleus to cytoplasm; mDia1 and HAN11 together repress DYRK1A-dependent GLI1 transcriptional activity. TAP-tag purification; GST pull-down; luciferase transcription assay; immunofluorescence microscopy Journal of dermatological science Medium 16887337
2007 mDia1 knockout mice develop lymphopenia with T cells showing impaired chemotaxis, reduced actin filament formation, impaired polarity in response to chemotactic stimuli, and poor adhesion to extracellular matrix; ERK1/2 activation is diminished in activated Drf1-/- T cells. Drf1 gene knockout mice; in vitro chemotaxis assays; actin staining; cell adhesion assays; flow cytometry; ERK phosphorylation western blot The Journal of experimental medicine High 17682067
2007 mDia1 loss (Drf1-/- mice) causes age-dependent myeloproliferative defects including splenomegaly, fibrotic hypercellular bone marrow, extramedullary hematopoiesis, and expansion of myeloid progenitors, resembling human myeloproliferative/myelodysplastic syndrome. Homologous recombination knockout mice; hematopoietic phenotyping; flow cytometry of surface markers; histology Cancer research High 17699759
2007 LARG (RhoGEF) and mDia1 link Gα12/13 signaling to MTOC polarity and microtubule dynamics during directed cell migration; LARG localizes to the MTOC via pericentrin and along microtubule tracks. Gα12/13-deficient mouse embryonic fibroblasts; LARG knockdown; dominant-negative mDia1; MTOC polarity assay; immunofluorescence Molecular biology of the cell Medium 17959834
2008 Memo acts upstream of RhoA-mDia1 to localize RhoA and mDia1 to the plasma membrane at the leading edge, coordinating lamellipodial actin network organization, adhesion site formation, and microtubule outgrowth during ErbB2-driven cell migration. Memo siRNA knockdown; immunofluorescence of RhoA/mDia1 localization; adhesion site live imaging; MT dynamics analysis The Journal of cell biology Medium 18955552
2008 G-actin accumulation acts as a physiological cue to enhance mDia1-catalyzed actin nucleation frequency via increased catalytic efficiency of its FH2 domain; this rapid restoration mechanism is distinct from Arp2/3 regulation. Single-molecule live-cell imaging of mDia1 in cells; latrunculin B and unpolymerizable actin mutant treatments; FH2-domain-only constructs; computational simulation of G-actin dynamics Journal of cell science High 18827014
2008 CLIP-170 directly interacts with the mDia1 FH2 domain, recruits mDia1 to the phagocytic cup during CR3-mediated phagocytosis, and thereby controls actin polymerization required for phagocytosis; this interaction is negatively regulated during phagocytosis. RNAi knockdown; dominant-negative approaches; co-immunoprecipitation; live imaging of CLIP-170 and mDia1 at phagocytic cup; phagocytosis efficiency assay The Journal of cell biology High 19114595
2009 mDia1 in NK cells mediates microtubule targeting to the lytic synapse (but not actin assembly there), enabling target cell lysis; loss of hDia1 perturbs the microtubule cytoskeleton without disrupting actin assembly at the lytic synapse. siRNA knockdown of Arp2/3 or hDia1 in NK cells; cytotoxicity assay; immunofluorescence of actin and microtubules at lytic synapse Current biology Medium 19913427
2009 Hck kinase forms a complex with mDia1 and WASp in chemoattractant-stimulated neutrophils; mDia1 is required for Hck membrane translocation, Hck activation, and Hck-mediated WASp tyrosine phosphorylation; mDia1-/- neutrophils show impaired Hck membrane targeting. Co-immunoprecipitation; mDia1-/- mouse neutrophils; immunofluorescence co-localization; Hck kinase activity assay; WASp phosphorylation western blot Biochemistry and cell biology Medium 19234535
2009 mDia1 translocates to the platelet cytoskeleton following thrombin stimulation in a PI 3-kinase-dependent manner; mDia1 is required for thrombin-induced actin stress fiber formation and platelet spreading; PI 3-kinase promotes mDia1-RhoA interaction. Anti-mDia1 antibody loading; PI 3-kinase inhibitors; co-immunoprecipitation of mDia1-RhoA; actin staining; platelet spreading assay on fibrinogen Biochemical and biophysical research communications Medium 19470376
2010 The cytoplasmic domain of RAGE (ctRAGE) contains an α-turn that mediates direct binding to mDia1; this ctRAGE-mDia1 interaction is essential for RAGE ligand-stimulated AKT phosphorylation and cell proliferation/migration. NMR solution structure of ctRAGE; NMR mapping of mDia1 binding interface; mutagenesis of α-turn; cell proliferation/migration assays The Journal of biological chemistry High 22194616
2010 mDia1 targets v-Src to the cell periphery/focal adhesions via actin filament generation; mDia1-deficient cells show impaired membrane translocation of v-Src, reduced tyrosine phosphorylation, and suppressed transformation, tumorigenesis, and invasion. mDia1 knockout mouse embryonic fibroblasts transduced with temperature-sensitive v-Src; immunofluorescence of Src localization; focus formation; soft agar colony assay; nude mouse xenograft Molecular and cellular biology High 20679479
2010 APC C-terminal basic domain nucleates actin filaments and synergizes with its in vivo binding partner mDia1 to overcome the dual cellular barrier imposed by profilin and capping protein; APC-mDia1 cooperation drives actin assembly in cells. In vitro pyrene-actin nucleation assays; EM of actin filaments; APC-mDia1 co-immunoprecipitation; cell-based actin assembly assay The Journal of cell biology High 20566685
2010 mDia1 crystal structure of a complex between N-terminal (DID/GBD) and C-terminal (FH2+DAD) fragments reveals two models for autoinhibition in which DAD engagement by N-terminus is incompatible with actin filament formation; nearly full-length mDia1 is dimeric. X-ray crystallography; analytical ultracentrifugation/gel filtration (oligomeric state); in-solution biochemical characterization PloS one High 20927338
2010 mDia1 rotates along the double helical strand of actin filament during processive elongation (helical rotation); this rotation is an intrinsic property of formins, independent of actin-bound nucleotide or profilin. Single-molecule fluorescence polarization of labeled F-actin elongating from immobilized mDia1; TIRF microscopy Science High 21148346
2010 Flightless-I (Fli-I) directly binds mDia1, enhances its intrinsic actin assembly activity in vitro, promotes GTP-Rho-mediated relief of mDia1 autoinhibition, and is required for Daam1/mDia1-induced actin assembly in living cells. Direct binding assay (GST pull-down); in vitro pyrene-actin polymerization; RNAi knockdown in cells; constitutively active Rho rescue The Journal of biological chemistry High 20223827
2010 ACTH-stimulated cortisol biosynthesis requires RhoA and DIAPH1 to mediate mitochondrial trafficking along microtubules; silencing DIAPH1 impairs mitochondrial movement and cortisol biosynthesis, increasing androgen secretion instead. Live cell video confocal microscopy; dominant-negative RhoA; DIAPH1 siRNA; cortisol/androgen secretion assays; co-immunoprecipitation of RhoA-DIAPH1 Endocrinology Medium 20591975
2010 The Rho-mDia1 pathway is required for dendritic cell adhesion, spreading, invasive and directional migration, and sustained T-cell interaction/stimulation; mDia1-/- DCs show impaired cutaneous migration to draining lymph nodes in vivo. mDia1-/- mice; in vitro adhesion/spreading assay; Transwell invasion assay; in vivo FITC-induced DC migration; DC-T cell interaction assay Blood High 20881208
2011 INF2, mDia1, and mDia2 all bind microtubules with high affinity via FH1-FH2-C constructs, but differ: mDia1 shows saturating binding at ~1:3 stoichiometry and is not a potent microtubule bundler; microtubule binding moderately inhibits mDia1 actin polymerization activity. In vitro microtubule co-sedimentation; TIRF microscopy; actin polymerization assay; stoichiometry analysis Molecular biology of the cell High 21998204
2011 Rho-mDia1 activation causes Golgi fragmentation into ministacks via actin polymerization; mDia1 transiently localizes to Rab6-positive Golgi-derived transport vesicles and promotes their formation; Golgi fusion is repressed by active mDia1. Constitutively active mDia1 expression; LPA stimulation; live imaging of Golgi markers; cytoskeletal inhibitors (latrunculin B, blebbistatin, taxol); GFP-mDia1 localization Molecular biology of the cell Medium 21680709
2011 mDia1 directly interacts with IRSp53 via IRSp53's SH3 domain and cooperates with WAVE2 to form filopodia; depletion of mDia1 or WAVE2 decreases IRSp53-induced filopodia formation; FRET confirms direct mDia1-IRSp53 interaction within filopodia. Co-immunoprecipitation; acceptor photobleaching FRET; siRNA knockdown; time-lapse live imaging The Journal of biological chemistry High 22179776
2011 Rif GTPase induces filopodia via mDia1 (not mDia2), through a pathway independent of Cdc42 effectors N-WASP and IRSp53; FRET confirms direct Rif-mDia1 interaction within filopodia. RNAi knockdown; dominant-negative mDia1; acceptor photobleaching FRET; time-lapse imaging The Journal of biological chemistry Medium 21339294
2012 Actin-capping protein induces stable detyrosinated microtubules in an mDia1-dependent manner by antagonizing mDia1 translocation on actin filament barbed ends, releasing mDia1 to act on microtubules. LatA/jasplakinolide treatment; mDia1 siRNA; capping protein siRNA; immunofluorescence of stable microtubules; live-cell mDia1 localization Molecular biology of the cell High 22918941
2012 mDia1 is required for RAGE-ligand-induced c-Src membrane translocation, Rac1 activation, redox phosphorylation of AKT/GSK3β, and vascular smooth muscle cell migration; mDia1 loss reduces pathological neointimal expansion after injury in vivo. mDia1 siRNA/KO; mDia1-/- mice with femoral artery denudation; c-Src membrane fractionation; Rac1-GTP pull-down; AKT/GSK3β phosphorylation western blot Circulation research High 22511750
2012 Abl kinases and mDia1 regulate caveolar domain organization and trafficking; mDia1 knockdown causes Cav1/caveolae clustering and prevents inward trafficking upon loss of adhesion; mDia1 acts downstream of Abl to control stress-fiber-linked Cav1 pool. mDia1 siRNA; constitutively active mDia1 rescue; live imaging of Cav1 and stress fibers; Abl-deficient cells Journal of cell science Medium 22454521
2014 DIAPH1 (mDia1) negatively regulates megakaryocyte proplatelet formation by controlling actin and microtubule cytoskeleton dynamics; combined inhibition of DIAPH1 and ROCK/myosin II synergistically increases proplatelet formation. mDia1 knockout megakaryocytes; ROCK inhibitor; fluorescence microscopy of actin and microtubules; proplatelet formation quantification Blood High 25298036
2014 mDia1 heterozygous and knockout granulocytes overexpress CD14 in a cell-autonomous manner, leading to hypersensitivity to LPS via CD14/TLR4 signaling; mDia1 deficiency downregulates membrane-associated genes specifically in granulocytes. mDia1 heterozygous and KO mice; flow cytometry; LPS stimulation assay; lenalidomide rescue; gene expression analysis Blood Medium 24891322
2015 mDia1 acts as an EGF-regulated actin nucleator that polymerizes linear filaments to activate the Arp2/3 complex, cooperating sequentially with Arp2/3 to initiate lamellipodia and ruffles; this cooperation is required for cell migration. mDia1 knockdown and rescue; optogenetics; pharmacological Arp2/3 inhibition; live-cell imaging of ruffles; TIRF imaging Journal of cell science High 26349808
2016 DIAPH1 R1213* gain-of-function variant (truncating the DAD C-terminus) disrupts DID-DAD autoinhibitory interaction, causing constitutive activation with increased cortical F-actin, stable microtubules in platelets, and reduced proplatelet formation from megakaryocytes. Exome sequencing; flow cytometry of platelet actin/tubulin; cultured megakaryocyte proplatelet formation; overexpression of R1213* in cells Blood High 26912466
2016 DIAPH1 c.3610C>T mutation (R1204X, within the basic RRKR motif of DAD) disrupts the DID-DAD autoinhibitory interaction, producing constitutively active DIA1 with increased directional actin polymerization rates and elongated microvilli; mice expressing this mutant develop progressive deafness and stereocilia morphological abnormalities. DID-DAD binding assay; single-molecule imaging of actin polymerization velocity; transgenic mouse model; auditory brainstem response; scanning electron microscopy of stereocilia EMBO molecular medicine High 27707755
2016 An mDia1-INF2 formin activation cascade, facilitated by IQGAP1 as a scaffold, is required for LPA-stimulated stable detyrosinated microtubule formation; mDia1 regulates INF2 localization to microtubules and their interaction is induced by LPA. siRNA knockdown of mDia1 and INF2; constitutively active formin mutants; IQGAP1 N-terminus direct binding to INF2 C-terminus (GST pull-down); immunofluorescence of Glu-MTs; co-immunoprecipitation Molecular biology of the cell High 27030671
2016 Small molecule inhibitors of the ctRAGE-DIAPH1 interaction were identified by screening 58,000 compounds; these inhibitors suppress RAGE-dependent signaling and biological activities in vitro and in vivo, confirming that the ctRAGE-DIAPH1 protein-protein interaction is required for RAGE signal transduction. High-throughput small molecule screen; NMR competition assay; X-ray crystallization of RAGE domains; cellular signaling assays; in vivo pharmacology Scientific reports High 26936329
2018 Helical rotation of mDia1 during processive actin elongation converts F-actin into a form resistant to cofilin binding and severing by untwisting the long-pitch actin helix; tethered (non-rotatable) mDia1 generates more cofilin-resistant F-actin in cells. Single-molecule fluorescence polarization; electron microscopy of F-actin twist; constitutively active tethered mDia1 mutant overexpression; cofilin-F-actin severing and binding assays Proceedings of the National Academy of Sciences of the United States of America High 29760064
2018 mDia1 and mDia3 together generate a dynamic cortical F-actin meshwork in Sertoli cells that is continuous with contractile actomyosin bundles and is required for Sertoli-germ cell interaction and spermatogenesis; mDia1/3 loss induces ectopic espin1-containing F-actin bundles. mDia1/mDia3 double knockout mice; superresolution and single-molecule imaging; spermatogenesis phenotyping; immunostaining of F-actin architectures PLoS biology High 30256801
2018 Diaph1 (mDia1) promotes TGFβ receptor II (TβRII) endocytosis and intracellular trafficking in hepatic stellate cells by interacting directly with both TβRII and Rab5a; Diaph1 increases Rab5a GTPase activity; Diaph1 inactivation blocks SMAD3 phosphorylation and myofibroblastic activation. shRNA knockdown; SMIFH2 inhibitor; co-immunoprecipitation of Diaph1-TβRII and Diaph1-Rab5a; active/inactive Rab5a mutants; endosomal localization assay; tumor implantation model FASEB journal High 32304339
2018 BIG2-ARF1-RhoA-mDia1 signaling axis regulates dendritic Golgi polarization and dendrite growth/maintenance in hippocampal neurons; mDia1 acts as downstream effector of RhoA in this pathway. shRNA knockdown; constitutively active ARF1/RhoA mutants; LPA treatment; immunofluorescence of Golgi markers; in utero electroporation Molecular neurobiology Medium 29455446
2019 Cdk1 phosphorylates DIAPH1, preventing profilin1 binding, to maintain metaphase cortical tension; DIAPH1 phosphorylation is required for kinetochore stretching and spindle assembly checkpoint (SAC) inactivation at anaphase onset. Cdk1 phosphorylation site mutants of DIAPH1; profilin1 binding assay; cortical tension measurement; SAC activation assay; intra-kinetochore distance measurement; live-cell imaging Nature communications High 30816115
2020 SPIN90 forms a ternary complex with Arp2/3 and mDia1, efficiently recruiting mDia1 to SPIN90-Arp2/3 nucleated filament pointed ends; this greatly enhances nucleation and yields rapidly elongating unbranched filaments, shifting cortical actin toward a formin-dominated network. In vitro reconstitution; TIRF microscopy; biochemical pull-down; in-cell actin network analysis Nature cell biology High 32572169
2020 mDia1 and mDia3 localize to the immune synapse upon TCR activation and are required for formin-mediated actin polymerization at the synapse, which spatiotemporally controls LAT phosphorylation by Zap70. mDia1 and mDia3 knockout mice; pharmacological formin inhibition; high-resolution imaging and 3D reconstruction of immune synapse; LAT and Zap70 phosphorylation assays Science advances High 31911947
2021 Loss of DIAPH1 causes mitochondrial respiratory chain complex IV dysfunction and combined immune deficiency, including defective lymphocyte maturation, poor T-cell activation, and inefficient MTOC repositioning to the immunological synapse. CRISPR-Cas9 DIAPH1 KO in PBMCs; patient-derived T cell assays; immunophenotyping by flow cytometry; mitochondrial complex IV activity assay; immunofluorescence of MTOC repositioning The Journal of allergy and clinical immunology High 33662367
2021 Tension-controlled actin polymerization at focal adhesions requires mDia1 and exhibits pulsatile dynamics triggered by contractile forces; suppression of mDia1-mediated actin polymerization increases stress fiber tension, raising the frequency of spontaneous SF damage and decreasing zyxin-mediated SF repair. Live-cell imaging of mDia1 at focal adhesions; traction force microscopy; mathematical modeling; mDia1 knockdown; stress fiber damage frequency quantification Developmental cell High 34822787
2021 Small-molecule antagonists of ctRAGE-DIAPH1 interaction (RAGE229) suppress RAGE-DIAPH1 binding (confirmed by FRET and NMR), reduce diabetic complications in mice including nephropathy and inflammation, without lowering blood glucose. NMR spectroscopy mapping of compound binding site; FRET; in vivo diabetic mouse models (STZ); histopathology; inflammatory cytokine measurement Science translational medicine High 34818060
2023 DIAPH1 mediates RAGE-dependent atherosclerosis progression and regulates hepatic lipid metabolism; DIAPH1 deletion reduces atherosclerosis and plasma cholesterol/triglycerides; DIAPH1 promotes SREBP1 nuclear translocation via the actin cytoskeleton, independently of canonical insulin/carbohydrate signaling. Ldlr-/- Diaph1-/- double-KO mice; Western diet feeding; lipid/cholesterol measurement; hepatic gene expression; SREBP1 nuclear translocation assay; actin cytoskeleton perturbation Communications biology High 36932214

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. The Journal of cell biology 1058 11402062
1999 Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nature cell biology 729 10559899
2009 Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion. Cancer metastasis reviews 436 19160018
2003 The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition. Current biology : CB 355 12906795
1997 Nonsyndromic deafness DFNA1 associated with mutation of a human homolog of the Drosophila gene diaphanous. Science (New York, N.Y.) 318 9360932
2001 Coordination of microtubules and the actin cytoskeleton by the Rho effector mDia1. Nature cell biology 273 11146620
2003 A conserved mechanism for Bni1- and mDia1-induced actin assembly and dual regulation of Bni1 by Bud6 and profilin. Molecular biology of the cell 235 14657240
2005 Structural basis of Rho GTPase-mediated activation of the formin mDia1. Molecular cell 209 15866170
2003 Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42. Current biology : CB 205 12676083
2002 ROCK and mDia1 antagonize in Rho-dependent Rac activation in Swiss 3T3 fibroblasts. The Journal of cell biology 181 12021256
2002 The diaphanous-related formin mDia1 controls serum response factor activity through its effects on actin polymerization. Molecular biology of the cell 160 12429848
2006 The Rho-mDia1 pathway regulates cell polarity and focal adhesion turnover in migrating cells through mobilizing Apc and c-Src. Molecular and cellular biology 152 16943426
2022 WTAP-mediated m6A modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis. Cell death and differentiation 141 34999731
2010 Adenomatous polyposis coli protein nucleates actin assembly and synergizes with the formin mDia1. The Journal of cell biology 137 20566685
2016 A gain-of-function variant in DIAPH1 causes dominant macrothrombocytopenia and hearing loss. Blood 121 26912466
2011 Differential interactions of the formins INF2, mDia1, and mDia2 with microtubules. Molecular biology of the cell 114 21998204
2006 Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLalpha and mDia1. The Journal of cell biology 114 16943183
2007 Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1. The Journal of experimental medicine 111 17682067
2001 Localization of a mammalian homolog of diaphanous, mDia1, to the mitotic spindle in HeLa cells. Journal of cell science 108 11171383
2011 Signal transduction in receptor for advanced glycation end products (RAGE): solution structure of C-terminal rage (ctRAGE) and its binding to mDia1. The Journal of biological chemistry 107 22194616
2007 T cell responses in mammalian diaphanous-related formin mDia1 knock-out mice. The Journal of biological chemistry 103 17595162
2016 Small Molecule Inhibition of Ligand-Stimulated RAGE-DIAPH1 Signal Transduction. Scientific reports 98 26936329
2008 Memo-RhoA-mDia1 signaling controls microtubules, the actin network, and adhesion site formation in migrating cells. The Journal of cell biology 97 18955552
2010 Rotational movement of the formin mDia1 along the double helical strand of an actin filament. Science (New York, N.Y.) 94 21148346
2008 The microtubule-binding protein CLIP-170 coordinates mDia1 and actin reorganization during CR3-mediated phagocytosis. The Journal of cell biology 87 19114595
2004 PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. The Journal of biological chemistry 87 15123714
2012 Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways. Circulation research 83 22511750
2011 mDia1 and WAVE2 proteins interact directly with IRSp53 in filopodia and are involved in filopodium formation. The Journal of biological chemistry 77 22179776
2007 Myeloproliferative defects following targeting of the Drf1 gene encoding the mammalian diaphanous related formin mDia1. Cancer research 71 17699759
2015 Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2/3 complex. Journal of cell science 70 26349808
2011 Involvement of the Rho-mDia1 pathway in the regulation of Golgi complex architecture and dynamics. Molecular biology of the cell 67 21680709
2014 Homozygous loss of DIAPH1 is a novel cause of microcephaly in humans. European journal of human genetics : EJHG 64 24781755
2014 The formin DIAPH1 (mDia1) regulates megakaryocyte proplatelet formation by remodeling the actin and microtubule cytoskeletons. Blood 64 25298036
2021 Small-molecule antagonism of the interaction of the RAGE cytoplasmic domain with DIAPH1 reduces diabetic complications in mice. Science translational medicine 61 34818060
2016 Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss. EMBO molecular medicine 61 27707755
2012 Actin-capping protein promotes microtubule stability by antagonizing the actin activity of mDia1. Molecular biology of the cell 60 22918941
2012 Caveolar domain organization and trafficking is regulated by Abl kinases and mDia1. Journal of cell science 53 22454521
2019 The Receptor for Advanced Glycation End Products (RAGE) and DIAPH1: Implications for vascular and neuroinflammatory dysfunction in disorders of the central nervous system. Neurochemistry international 52 30902646
2011 Role of RhoA and its effectors ROCK and mDia1 in the modulation of deformation-induced FAK, ERK, p38, and MLC motogenic signals in human Caco-2 intestinal epithelial cells. American journal of physiology. Cell physiology 50 21849669
2016 An mDia1-INF2 formin activation cascade facilitated by IQGAP1 regulates stable microtubules in migrating cells. Molecular biology of the cell 49 27030671
2021 DIAPH1 Variants in Non-East Asian Patients With Sporadic Moyamoya Disease. JAMA neurology 47 34125151
2009 Distinct roles for the actin nucleators Arp2/3 and hDia1 during NK-mediated cytotoxicity. Current biology : CB 47 19913427
2020 SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization. Nature cell biology 46 32572169
2016 Extension of the clinical and molecular phenotype of DIAPH1-associated autosomal dominant hearing loss (DFNA1). Clinical genetics 46 27808407
2010 Rho-mDia1 pathway is required for adhesion, migration, and T-cell stimulation in dendritic cells. Blood 46 20881208
2008 G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers. Journal of cell science 46 18827014
2013 The formins FMNL1 and mDia1 regulate coiling phagocytosis of Borrelia burgdorferi by primary human macrophages. Infection and immunity 45 23460512
2007 LARG and mDia1 link Galpha12/13 to cell polarity and microtubule dynamics. Molecular biology of the cell 45 17959834
2021 Loss of DIAPH1 causes SCBMS, combined immunodeficiency, and mitochondrial dysfunction. The Journal of allergy and clinical immunology 44 33662367
2015 Novel loss-of-function variants in DIAPH1 associated with syndromic microcephaly, blindness, and early onset seizures. American journal of medical genetics. Part A 43 26463574
2014 Aberrant overexpression of CD14 on granulocytes sensitizes the innate immune response in mDia1 heterozygous del(5q) MDS. Blood 43 24891322
2001 Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton. Journal of cell science 43 11707518
2010 Crystal structure of a complex between amino and carboxy terminal fragments of mDia1: insights into autoinhibition of diaphanous-related formins. PloS one 42 20927338
2018 Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin. Proceedings of the National Academy of Sciences of the United States of America 37 29760064
2011 Rif-mDia1 interaction is involved in filopodium formation independent of Cdc42 and Rac effectors. The Journal of biological chemistry 36 21339294
2010 Flightless-I (Fli-I) regulates the actin assembly activity of diaphanous-related formins (DRFs) Daam1 and mDia1 in cooperation with active Rho GTPase. The Journal of biological chemistry 36 20223827
2017 The Formin, DIAPH1, is a Key Modulator of Myocardial Ischemia/Reperfusion Injury. EBioMedicine 35 29239839
1998 Further characterization of the DFNA1 audiovestibular phenotype. Archives of otolaryngology--head & neck surgery 33 9639482
2016 HYDROGEN-RICH MEDIUM AMELIORATES LIPOPOLYSACCHARIDE-INDUCED BARRIER DYSFUNCTION VIA RHOA-MDIA1 SIGNALING IN CACO-2 CELLS. Shock (Augusta, Ga.) 32 26529665
2010 RhoA and DIAPH1 mediate adrenocorticotropin-stimulated cortisol biosynthesis by regulating mitochondrial trafficking. Endocrinology 31 20591975
2020 FPS-ZM1 Alleviates Neuroinflammation in Focal Cerebral Ischemia Rats via Blocking Ligand/RAGE/DIAPH1 Pathway. ACS chemical neuroscience 30 33300334
2018 Phenotype description and response to thrombopoietin receptor agonist in DIAPH1-related disorder. Blood advances 30 30232087
2007 Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor. Biology of reproduction 30 17301291
2013 Expression of DIAPH1 is up-regulated in colorectal cancer and its down-regulation strongly reduces the metastatic capacity of colon carcinoma cells. International journal of cancer 29 24105619
2018 Mdia1 is Crucial for Advanced Glycation End Product-Induced Endothelial Hyperpermeability. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 26 29490301
2018 IP3R3 silencing induced actin cytoskeletal reorganization through ARHGAP18/RhoA/mDia1/FAK pathway in breast cancer cell lines. Biochimica et biophysica acta. Molecular cell research 26 29630900
2016 Glycation & the RAGE axis: targeting signal transduction through DIAPH1. Expert review of proteomics 26 27967251
2021 AGE/RAGE/DIAPH1 axis is associated with immunometabolic markers and risk of insulin resistance in subcutaneous but not omental adipose tissue in human obesity. International journal of obesity (2005) 25 34103691
2014 Formin mDia1, a downstream molecule of FMNL1, regulates Profilin1 for actin assembly and spindle organization during mouse oocyte meiosis. Biochimica et biophysica acta 25 25447542
2006 HAN11 binds mDia1 and controls GLI1 transcriptional activity. Journal of dermatological science 25 16887337
2021 Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair. Developmental cell 24 34822787
2019 DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma. Disease markers 24 30733838
2018 Deletion of the formin Diaph1 protects from structural and functional abnormalities in the murine diabetic kidney. American journal of physiology. Renal physiology 24 30132346
2018 BIG2-ARF1-RhoA-mDia1 Signaling Regulates Dendritic Golgi Polarization in Hippocampal Neurons. Molecular neurobiology 22 29455446
2017 Progressive macrothrombocytopenia and hearing loss in a large family with DIAPH1 related disease. American journal of medical genetics. Part A 22 28815995
2017 Knockdown of Diaph1 expression inhibits migration and decreases the expression of MMP2 and MMP9 in human glioma cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 22 29035824
2010 mDia1 targets v-Src to the cell periphery and facilitates cell transformation, tumorigenesis, and invasion. Molecular and cellular biology 21 20679479
2019 Mammalian diaphanous-related formin 1 (mDia1) coordinates mast cell migration and secretion through its actin-nucleating activity. The Journal of allergy and clinical immunology 20 31279009
2018 Enteropathogenic E. coli relies on collaboration between the formin mDia1 and the Arp2/3 complex for actin pedestal biogenesis and maintenance. PLoS pathogens 20 30550556
2022 The RAGE/DIAPH1 Signaling Axis & Implications for the Pathogenesis of Diabetic Complications. International journal of molecular sciences 19 35562970
2018 Diaphanous 1 (DIAPH1) is Highly Expressed in the Aged Human Medial Temporal Cortex and Upregulated in Myeloid Cells During Alzheimer's Disease. Journal of Alzheimer's disease : JAD 19 29966194
2018 mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. PLoS biology 19 30256801
2018 mDia1 and Cdc42 Regulate Activin B-Induced Migration of Bone Marrow-Derived Mesenchymal Stromal Cells. Stem cells (Dayton, Ohio) 19 30358011
2023 DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice. Communications biology 18 36932214
2020 Association of DIAPH1 gene polymorphisms with ischemic stroke. Aging 18 31899686
2020 A novel variant in diaphanous homolog 1 (DIAPH1) as the cause of auditory neuropathy in a Chinese family. International journal of pediatric otorhinolaryngology 18 32087478
2020 CREB1-induced lncRNA LEF1-AS1 contributes to colorectal cancer progression via the miR-489/DIAPH1 axis. Biochemical and biophysical research communications 18 32248974
2020 Protein diaphanous homolog 1 (Diaph1) promotes myofibroblastic activation of hepatic stellate cells by regulating Rab5a activity and TGFβ receptor endocytosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18 32304339
2012 mDia1-3 in mammalian filopodia. Communicative & integrative biology 18 23060957
2009 Src kinase Hck association with the WASp and mDia1 cytoskeletal regulators promotes chemoattractant-induced Hck membrane targeting and activation in neutrophils. Biochemistry and cell biology = Biochimie et biologie cellulaire 18 19234535
2009 Loss of RhoB expression enhances the myelodysplastic phenotype of mammalian diaphanous-related Formin mDia1 knockout mice. PloS one 18 19768111
2021 The cross-talk between RAGE and DIAPH1 in neurological complications of diabetes: A review. The European journal of neuroscience 17 34449932
2019 RHOA and mDia1 Promotes Apoptosis of Breast Cancer Cells Via a High Dose of Doxorubicin Treatment. Open life sciences 17 33817200
2015 Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Oncotarget 17 26124177
2022 Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy. Clinical immunology (Orlando, Fla.) 16 36503156
2020 mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse. Science advances 16 31911947
2019 Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase. Nature communications 16 30816115
2015 Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK. PloS one 16 26674774
2016 A novel missense variant in the DIAPH1 gene in a Korean family with autosomal dominant nonsyndromic hearing loss. Genes & genetic systems 15 28003573
2009 RhoA effector mDia1 is required for PI 3-kinase-dependent actin remodeling and spreading by thrombin in platelets. Biochemical and biophysical research communications 15 19470376