Affinage

IQGAP1

Ras GTPase-activating-like protein IQGAP1 · UniProt P46940

Length
1657 aa
Mass
189.3 kDa
Annotated
2026-06-10
100 papers in source corpus 55 papers cited in narrative 55 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

IQGAP1 is a ubiquitously expressed multidomain scaffold that integrates actin cytoskeletal dynamics with Rho-family GTPase signaling to coordinate cell polarity, migration, adhesion, and division (PMID:8670801, PMID:12900413). Through its GAP-related domain it binds selectively to the GTP-bound forms of Cdc42 and Rac1—but not Ras—and inhibits their intrinsic GTPase activity, thereby stabilizing the active state of these GTPases rather than accelerating their inactivation (PMID:8670801, PMID:17984089); it similarly engages GTP-loaded RhoA/RhoC and Rap1 (PMID:22992742, PMID:17517894). IQGAP1 directly binds and cross-links F-actin via its calponin homology domain, and as a dimer it caps barbed ends, organizes filaments into bundles, and stimulates Arp2/3-dependent branched nucleation by activating N-WASP, linking receptor inputs such as FGFR1 to lamellipodial actin assembly (PMID:9199170, PMID:34731043, PMID:17085436, PMID:17264147). By capturing microtubule plus-ends at the cell cortex through a tripartite complex with activated Cdc42/Rac1 and CLIP-170, it generates polarized microtubule arrays that direct migration (PMID:12110184). A central output is its role as the scaffold of the Ras/MAPK module, binding B-Raf, MEK1/2, and ERK1/2 to enable EGF- and growth-factor-stimulated kinase activation (PMID:14970219, PMID:16135787, PMID:18567582); disrupting the IQGAP1–ERK interaction with a WW-domain peptide blocks RAS/RAF-driven tumorigenesis and overcomes BRAF-inhibitor resistance (PMID:23603816). Beyond MAPK, IQGAP1 scaffolds AMPK/CaMKK2, the insulin receptor/IRS-1, VEGFR2, EGFR, and HER2, and acts as a negative regulator of Hippo/YAP signaling by binding YAP and the MST2–LATS1 kinases (PMID:33191271, PMID:28082684, PMID:32783108, PMID:21349850, PMID:21724847, PMID:27440047, PMID:33672268). Its many interactions are gated by Ca2+/calmodulin, which competes with F-actin, Cdc42, E-cadherin, Rap1, B-Raf and EGFR for distinct IQGAP1 domains (PMID:9867866, PMID:10608854, PMID:18567582, PMID:21349850), and by post-translational modifications including PKCε/PKCα phosphorylation at Ser-1443, GLK-mediated Ser-480 phosphorylation, K1445 SUMOylation, and ubiquitination within the GAP-related domain that tunes its GTPase binding and stability (PMID:15695813, PMID:21349850, PMID:31431460, PMID:28987385, PMID:32094223, PMID:28073378). IQGAP1 also supports immune effector functions, including NK-cell MTOC polarization and cytotoxicity and the calibration of T-cell activation (PMID:21681737, PMID:22573807).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1996 High

    Established the founding biochemical identity of IQGAP1 as a Cdc42/Rac1-selective binding protein that inhibits their GTPase activity and associates with calmodulin and cortical actin, distinguishing it from Ras-targeting regulators.

    Evidence Affinity pulldown, GTPase activity assay, Co-IP, and immunofluorescence in a foundational study

    PMID:8670801

    Open questions at the time
    • Did not resolve whether IQGAP1 acts as a true GAP or an effector that stabilizes GTP-bound state
    • structural basis of selectivity for Cdc42/Rac1 over Ras not defined
  2. 1997 High

    Defined IQGAP1 as a direct F-actin cross-linker, providing the structural basis for its cytoskeletal scaffolding function.

    Evidence F-actin co-sedimentation, electron microscopy with purified bovine IQGAP1

    PMID:9199170

    Open questions at the time
    • Single-filament kinetics of actin organization not yet resolved
    • in vivo consequence of cross-linking not tested
  3. 1999 High

    Showed that Ca2+/calmodulin is a master switch dissociating IQGAP1 from Cdc42, F-actin and E-cadherin, establishing calmodulin as a competitive regulator that toggles IQGAP1 between signaling and adhesion roles.

    Evidence In vitro competition binding, GTPase assays, and adhesion assays in MCF-7/MDA-MB-231 cells

    PMID:10608854 PMID:9867866

    Open questions at the time
    • Quantitative relationship between local Ca2+ flux and partner exchange in vivo not defined
    • did not address how calmodulin coordinates simultaneous competing partners
  4. 2002 High

    Resolved how IQGAP1 links GTPase activation to microtubule capture, defining a Cdc42/Rac1–IQGAP1–CLIP-170 tripartite complex that polarizes the microtubule array for directed migration.

    Evidence Co-IP, mutant constructs, and GFP-CLIP-170 live imaging in Vero fibroblasts

    PMID:12110184

    Open questions at the time
    • Stoichiometry and assembly order of the tripartite complex unresolved
    • how the complex is spatially restricted to the leading edge not defined
  5. 2003 High

    Demonstrated that IQGAP1 levels bidirectionally control cell migration and invasion in a Cdc42/Rac1-dependent manner, connecting its biochemistry to a functional motility phenotype.

    Evidence siRNA knockdown, dominant-negative GRD construct, transwell migration/invasion assays

    PMID:12900413

    Open questions at the time
    • Did not separate scaffolding from actin cross-linking contributions to motility
    • in vivo invasion not tested in this study
  6. 2007 High

    Established IQGAP1 as an activator of Arp2/3-mediated branched actin nucleation through N-WASP, with intramolecular autoinhibition, linking receptor signaling (FGFR1) to lamellipodial actin assembly.

    Evidence Reconstituted actin polymerization with purified proteins/Arp2/3, Co-IP, siRNA, migration assays

    PMID:17085436 PMID:17264147

    Open questions at the time
    • Physiological signal that relieves intramolecular autoinhibition not identified
    • interplay between cross-linking and nucleation activities not resolved
  7. 2005 High

    Identified IQGAP1 as the scaffold of the Ras/MAPK cascade, binding ERK2, MEK1/2 and B-Raf and required for growth-factor-stimulated kinase activation, defining its central signaling output.

    Evidence Direct in vitro binding, Co-IP, bidirectional manipulation, EGF/IGF-I stimulation kinase assays

    PMID:14970219 PMID:16135787 PMID:18567582

    Open questions at the time
    • How EGF differentially partitions MEK1 versus MEK2 binding mechanistically unresolved
    • stoichiometry of the assembled MAPK module on IQGAP1 not defined
  8. 2005 High

    Showed IQGAP1 self-association (residues 763–863) and PKCε phosphorylation at Ser-1443 are required for its cellular functions, establishing oligomerization and phosphorylation as regulatory layers.

    Evidence Co-IP, gel filtration, competing peptide, MS phosphosite ID, kinase assay, neurite outgrowth in N1E-115 cells

    PMID:15695813 PMID:16105843

    Open questions at the time
    • Structural form of physiologically active oligomer unresolved
    • the upstream signal triggering self-association in cells not defined
  9. 2011 High

    Extended IQGAP1's receptor scaffolding to EGFR and HER2, showing it is required for receptor autophosphorylation and signaling, with phosphorylation- and calmodulin-gated control and therapeutic relevance to trastuzumab resistance.

    Evidence Direct in vitro binding, null-cell reconstitution, MS phosphosite, knockdown, drug-sensitivity assays

    PMID:21349850 PMID:21724847

    Open questions at the time
    • Whether IQGAP1 directly enhances receptor kinase activity or stabilizes receptor not fully separated
    • structural basis of IQ-domain/receptor binding not defined
  10. 2013 High

    Validated IQGAP1 scaffold disruption as a therapeutic strategy, showing a WW-domain peptide blocks RAS/RAF-driven tumors and bypasses BRAF-inhibitor resistance in vivo.

    Evidence WW-domain peptide competition, IQGAP1-null tumor model, in vivo survival studies

    PMID:23603816

    Open questions at the time
    • Generality across additional RAS/RAF-driven tumor types not fully mapped
    • off-pathway effects of WW-peptide on other IQGAP1 functions not characterized
  11. 2016 High

    Defined IQGAP1 as a negative regulator of Hippo/YAP output, directly binding YAP and later the MST2–LATS1 kinases to suppress TEAD-dependent transcription, broadening its role beyond MAPK.

    Evidence Direct binding, IQGAP1-null MEFs/CRISPR KD, TEAD reporter, kinase assays

    PMID:27440047 PMID:33672268

    Open questions at the time
    • How IQGAP1 simultaneously coordinates Hippo, MAPK and adhesion modules unresolved
    • context determining activation versus suppression of YAP not defined
  12. 2017 High

    Established a post-translational code governing IQGAP1 stability and GTPase binding, with SUMOylation at K1445, GLK-mediated Ser-480 phosphorylation, and ubiquitination within the GRD all tuning Cdc42/Rac1 activation and migration.

    Evidence Site mutagenesis, MS site identification, ubiquitination assays, null-cell reconstitution, in vivo metastasis models

    PMID:28073378 PMID:28987385 PMID:31431460 PMID:32094223 PMID:36870986

    Open questions at the time
    • Hierarchy and cross-talk among the modifications not integrated
    • the enzymes responsible for several modifications only partially identified
  13. 2020 High

    Expanded IQGAP1's scaffolding to metabolic and vascular signaling, showing it directly assembles AMPK/CaMKK2 and IR/IRS-1 modules and sustains VEGFR2–Rac1 signaling, with in vivo glucose-homeostasis and angiogenesis phenotypes.

    Evidence Direct binding, Co-IP, IQGAP1-null mice, rescue, kinase and GTPase activation assays

    PMID:28082684 PMID:32783108 PMID:33191271

    Open questions at the time
    • How a single scaffold partitions among competing metabolic and growth modules unresolved
    • tissue-specific selectivity of these complexes not defined
  14. 2022 High

    Connected IQGAP1 to inflammasome-driven secretion, showing CDC42-activated IQGAP1 bridges GSDMD to ESCRT/Tsg101 for exosomal pro-IL-1β release, illustrating a non-migratory effector role.

    Evidence Unbiased proteomics, Co-IP, knockdown, exosome isolation, NLRP3 activation, CDC42 activation assay

    PMID:36373462

    Open questions at the time
    • Whether this is a general route for cargo secretion beyond IL-1β not established
    • structural basis of GSDMD–IQGAP1–Tsg101 bridging unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How IQGAP1 dynamically prioritizes and spatially segregates its many competing partners—across Rho GTPases, MAPK, Hippo, metabolic and immune modules—into discrete functional outcomes within a single cell remains unresolved.
  • No structural model of full-length multidomain IQGAP1 with multiple partners
  • quantitative rules governing partner exchange under physiological Ca2+/PTM states not established
  • spatial organization of distinct IQGAP1 signalosomes within one cell not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 7 GO:0098772 molecular function regulator activity 5 GO:0008092 cytoskeletal protein binding 3 GO:0005198 structural molecule activity 2
Localization
GO:0005886 plasma membrane 4 GO:0005815 microtubule organizing center 3 GO:0005856 cytoskeleton 3 GO:0005829 cytosol 2 GO:0005768 endosome 1
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-168256 Immune System 6 R-HSA-1266738 Developmental Biology 4 R-HSA-1430728 Metabolism 3 R-HSA-5653656 Vesicle-mediated transport 2
Partners
BRAFCALM1CDC42CLIP-170EGFRMAP2K1MAPK1RAC1
Complex memberships
Cdc42/Rac1–IQGAP1–CLIP-170 microtubule-capture complexRas/MAPK scaffold module (B-Raf/MEK1/2/ERK1/2)

Evidence

Reading pass · 55 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 IQGAP1 (p195) binds directly to GTP-bound Cdc42 and Rac1 but not to Ras, inhibiting their intrinsic GTPase activity; the C-terminal half containing the GRD mediates this interaction in a GRD-dependent fashion. Calmodulin co-immunoprecipitates with IQGAP1 via its IQ domain. IQGAP1 localizes to lamellipodia and ruffling membranes where it co-localizes with actin. Affinity chromatography pulldown, Co-IP, GTPase activity assay, yeast functional complementation, immunofluorescence The EMBO journal High 8670801
1997 Purified native IQGAP1 directly binds F-actin and cross-links actin filaments into bundles with gel-like properties. Exogenous calmodulin (especially Ca2+-free form) partially inhibits IQGAP1 binding to F-actin, acting at the calponin homology domain. Protein purification from bovine adrenal, F-actin co-sedimentation assay, electron microscopy, immunofluorescence with cytochalasin D The Journal of cell biology High 9199170
1999 Ca2+/calmodulin dissociates Cdc42 from IQGAP1 and abrogates IQGAP1's inhibition of Cdc42 GTPase activity. Calmodulin binds both the calponin homology domain and IQ motifs of IQGAP1; F-actin competes with Ca2+/calmodulin for the calponin homology domain only. Increasing Ca2+ enhances calmodulin–IQGAP1 association with concomitant decrease in Cdc42–IQGAP1 association. In vitro binding assays with purified proteins, GTPase activity assay, cell lysate co-immunoprecipitation The Journal of biological chemistry High 9867866
1999 Calmodulin and E-cadherin compete for binding to IQGAP1 in vitro and in cells. E-cadherin is required for accumulation of IQGAP1 at cell–cell junctions. Calmodulin antagonism increases IQGAP1 at cell–cell contacts and decreases E-cadherin there, impairing homophilic E-cadherin adhesion. In vitro competition binding assay, immunocytochemistry in MCF-7 and MDA-MB-231 cells, calmodulin antagonist (CGS9343B) treatment, cell adhesion assay The Journal of biological chemistry High 10608854
2002 IQGAP1 interacts with CLIP-170, and activated Rac1/Cdc42 form a tripartite complex with IQGAP1 and CLIP-170 to capture microtubule plus ends at the cell cortex, generating a polarized microtubule array. Expression of a C-terminal IQGAP1 fragment delocalizes CLIP-170 from microtubule tips and alters the microtubule array; an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. Co-IP, dominant-negative and mutant construct expression, GFP-CLIP-170 live imaging, immunofluorescence in Vero fibroblasts Cell High 12110184
2003 IQGAP1 overexpression enhances cell migration and invasion in a Cdc42- and Rac1-dependent manner; knockdown of endogenous IQGAP1 by siRNA significantly decreases cell motility and invasion. A dominant-negative construct lacking the GRD (IQGAP1ΔGRD) also inhibits invasion mediated by constitutively active Cdc42. siRNA knockdown, dominant-negative construct transfection, transwell migration and invasion assays The Journal of biological chemistry High 12900413
2003 IQGAP1 associates with AKAP79, which bridges PKA to the C-terminal domain of IQGAP1, forming an IQGAP1/AKAP79/PKA complex in beta-cells and linking cAMP/PKA signaling to Ca2+/calmodulin and GTPase pathways. cAMP affinity chromatography, co-immunoprecipitation, in vitro binding with GST fusion proteins Journal of cellular biochemistry Medium 12938160
2004 ERK2 binds directly to IQGAP1 in vitro; endogenous ERK2 co-immunoprecipitates with IQGAP1 in cells. Manipulation of IQGAP1 levels significantly reduces EGF- and IGF-I-stimulated ERK1/2 activity; an IQGAP1 construct lacking the ERK2-binding region does not affect EGF-stimulated ERK activation. In vitro binding with purified proteins, Co-IP, IQGAP1 overexpression/knockdown, ERK kinase activity assays The Journal of biological chemistry High 14970219
2005 Both MEK1 and MEK2 bind directly to IQGAP1 in vitro and co-immunoprecipitate with it; ERK2 enhances MEK2–IQGAP1 interaction fourfold in vitro. EGF differentially regulates binding: enhancing MEK1 and reducing MEK2 interaction. Both knockdown and overexpression of IQGAP1 reduce EGF-stimulated MEK and ERK activation, establishing IQGAP1 as a scaffold for the Ras/MAPK cascade. In vitro binding, Co-IP, siRNA knockdown, EGF stimulation assays, selective mutant constructs Molecular and cellular biology High 16135787
2005 IQGAP1 self-associates, forming monomers, dimers, and larger oligomers; the self-association region maps to amino acids 763–863. Self-association is required for IQGAP1 to maintain active Cdc42 levels in cells; deletion of this region or competing peptide abolishes Cdc42 activation. Co-IP in cells, in vitro binding, gel filtration, competing peptide assay, active Cdc42 pulldown The Journal of biological chemistry High 16105843
2005 IQGAP1 is phosphorylated at Ser-1443 (and Ser-1441) by protein kinase Cε in vitro; Ser-1443 is the major site phosphorylated in intact cells after PMA stimulation. A non-phosphorylatable IQGAP1 S1441A/S1443A fails to promote neurite outgrowth, whereas the phosphomimetic S1441E/S1443D markedly enhances it, demonstrating phosphorylation-dependent regulation of IQGAP1 cytoskeletal function. Mass spectrometry phosphosite identification, in vitro kinase assay with purified PKCε, phosphomimetic and non-phosphorylatable mutant constructs, neurite outgrowth assay in N1E-115 cells The Journal of biological chemistry High 15695813
2006 IQGAP1 stimulates Arp2/3-dependent actin assembly by activating N-WASP through its C-terminal half (interacting with the BR-CRIB domain of N-WASP in a Cdc42-like manner); the N-terminal half of IQGAP1 antagonizes this activation via intramolecular binding. Signal-induced relief of autoinhibition is proposed to allow N-WASP activation. Pulldown, Co-IP, kinetic actin polymerization assays with purified proteins and Arp2/3 complex, quantitative co-localization analysis, IQGAP1 siRNA knockdown The Journal of biological chemistry High 17085436
2007 IQGAP1 interacts with the Diaphanous-related formin Dia1 through a region within the Diaphanous inhibitory domain after RhoA-mediated release of Dia1 autoinhibition; this interaction is required for Dia1 subcellular localization, phagocytic cup formation, and phagocytosis in macrophages. Co-IP, pulldown, immunofluorescence co-localization, siRNA knockdown, phagocytosis assays The Journal of cell biology High 17620407
2007 IQGAP1 binds directly to the cytoplasmic tail of FGFR1 and to N-WASP; FGF2 stimulation promotes association of IQGAP1 with FGFR1, N-WASP, and Arp2/3 complex in lamellipodia. IQGAP1 stimulates branched actin filament nucleation in vitro in the presence of N-WASP and Arp2/3, linking FGF2 receptor signaling to actin assembly. In vitro binding with purified proteins, Co-IP, immunofluorescence, actin polymerization assay, siRNA knockdown, cell migration assay Journal of cell science High 17264147
2007 IQGAP1 binds directly to Rap1; GTP-loading of Rap1 augments this interaction. Calmodulin (with or without Ca2+) eliminates Rap1–IQGAP1 binding, mapped to the IQ region of IQGAP1. Overexpression of IQGAP1 reduces adhesion- and cAMP-mediated Rap1 activation, while IQGAP1 loss enhances it. In vitro binding with purified proteins, Co-IP, confocal microscopy, gain- and loss-of-function manipulation, Rap1 activation assay The Journal of biological chemistry High 17517894
2007 Actin binding (via the calponin homology domain) is essential for IQGAP1 to stimulate cell migration; elimination of Ca2+/calmodulin binding augments IQGAP1-stimulated migration. Both Cdc42 and Rac1 contribute to IQGAP1-stimulated migration. IQGAP1 point mutant constructs, calmodulin inhibitor peptide, wound-healing and transwell migration assays, immunofluorescence Cellular signalling Medium 17544257
2007 Rac1 and Cdc42 use distinct switch-region residues to bind IQGAP1: switch I residues 32 and 36 are important for both; switch II mutations at Asp-63, Arg-68, or Leu-70 abrogate Rac1 binding but do not affect Cdc42 binding. The Rho insert loop does not contribute to IQGAP1 binding. Binding sites for IQGAP1 and RhoGAP on Rac1/Cdc42 only partially overlap. Site-directed mutagenesis of Rac1/Cdc42, affinity measurements, thermodynamic analysis, competition assays The Journal of biological chemistry High 17984089
2008 Ca2+ directly promotes IQGAP1 binding to B-Raf in vitro; calmodulin inhibits this interaction in a Ca2+-regulated manner. Chelation of intracellular Ca2+ enhances EGF-stimulated B-Raf activity in an IQGAP1-dependent manner; EGF promotes B-Raf–IQGAP1 association in cells; Ca2+ ionophores reduce this co-immunoprecipitation. In vitro binding with purified proteins, Co-IP, calmodulin competition, Ca2+ ionophore and chelator treatment, B-Raf kinase activity assay The Journal of biological chemistry High 18567582
2008 IQGAP1 is required for VEGFR2-mediated signaling: c-Src phosphorylates IQGAP1 on tyrosine and acts as an adaptor bridging IQGAP1 to VEGFR2; IQGAP1 then activates b-Raf and mediates endothelial cell proliferation and angiogenesis. Co-IP (SH2 domain pulldown), siRNA knockdown of IQGAP1 and b-Raf, in vivo CAM angiogenesis assay, kinase assays PloS one Medium 19050761
2008 Cdc42 acts upstream of IQGAP1 in mouse oocytes: Toxin B inhibition of Cdc42 relocates IQGAP1, inhibits polar body emission, and abolishes cortical actin without affecting meiotic spindle migration. IQGAP1 concentrates in the contractile ring during cytokinesis. Toxin B treatment, immunofluorescence, confocal microscopy in mouse oocytes and embryos Developmental biology Medium 18662680
2009 IQGAP1 interacts with mTOR through its N-terminus; this interaction is required for IQGAP1-mediated cell proliferation. The N-terminus increases cell size while the C-terminus reduces it, suggesting IQGAP1 is a phosphorylation-sensitive conformational switch coupling cell growth and division via a CDC42–mTOR pathway. Domain-specific fragment expression, Co-IP, cell size measurements, proliferation and transformation assays Journal of cell science Medium 19454477
2011 IQGAP1 binds directly to EGFR through its IQ domain (and the EGFR kinase domain); calmodulin disrupts this interaction. EGF induces IQGAP1 Ser-1443 phosphorylation via PKCα downstream of EGFR. In IQGAP1-null cells, EGF-stimulated EGFR autophosphorylation is severely attenuated and restored by reconstituting wild-type IQGAP1; the S1443D phosphomimetic enhances it. Co-IP, in vitro binding domain mapping, MS-based phosphorylation assay, IQGAP1-null cell reconstitution with WT and S1443D mutant The Journal of biological chemistry High 21349850
2011 IQGAP1 binds directly to HER2; knockdown of IQGAP1 decreases HER2 expression, phosphorylation, and signaling; these effects are reversed by IQGAP1 reconstitution. IQGAP1 is overexpressed in trastuzumab-resistant breast cells, and reducing IQGAP1 restores trastuzumab sensitivity. In vitro binding with purified proteins, siRNA knockdown, reconstitution, immunoprecipitation, cell proliferation assays The Journal of biological chemistry High 21724847
2011 IQGAP1 binds c-Raf, MEK1/2, ERK1/2, and AKT in the heart (pulldown). IQGAP1-null mice show impaired MEK1/2–ERK1/2 and AKT phosphorylation at 4 days (but not 10 min) after aortic banding, leading to accelerated adverse cardiac remodeling including impaired cardiomyocyte hypertrophy and increased apoptosis. Pulldown, IQGAP1-null mouse model, pressure overload (aortic banding), phosphorylation analysis, cardiac histology Cardiovascular research High 21493702
2011 IQGAP1 is required for MTOC polarization to the NK immune synapse and for perigranular F-actin network formation; IQGAP1 silencing abolishes YTS NK cell cytotoxic activity without preventing conjugate formation. siRNA silencing, immunofluorescence, confocal microscopy, cytotoxicity assay in YTS and primary NK cells European journal of immunology Medium 21681737
2011 IQGAP1-deficient CD8+ T cells are hyperresponsive, showing increased IL-2/IFN-γ production, heightened LCK activation, augmented global phosphorylation after TCR ligation, increased F-actin assembly, and amplified F-actin velocities during spreading. Discrete IQGAP1 regions regulate activation and F-actin accumulation via distinct mechanisms. IQGAP1-null and siRNA-knockdown T cells, IQGAP1 domain constructs, cytokine ELISA, phospho-flow cytometry, live-cell F-actin imaging Journal of immunology High 22573807
2011 IQGAP1 and CLIP-170 cooperate to regulate dendritic arbor morphology; mTOR kinase interacts with CLIP-170 and is needed for efficient CLIP-170–IQGAP1 complex formation. Dynamic microtubules, CLIP-170, and IQGAP1 regulate dendritic arbor growth via actin cytoskeleton regulation. Co-IP, siRNA knockdown of CLIP-170 and IQGAP1 in rat neurons, confocal morphometry, mTOR inhibitor treatment The Journal of neuroscience Medium 21430156
2012 IQGAP1 interacts directly with GTP-bound, prenylated RhoA and RhoC but not RhoB, acting both upstream (stabilizing Rho-GTP) and downstream of RhoA/C to mediate proliferation and migration in breast cancer cells, respectively. Proteomics screen, Co-IP, siRNA knockdown, active Rho pulldown, adenoviral constitutively active RhoA, DNA synthesis assay, migration assay The Journal of biological chemistry High 22992742
2013 IQGAP1 binds TβRII and suppresses TGF-β signaling in hepatic stellate cells by targeting the E3 ligase SMURF1 to the plasma membrane, promoting TβRII ubiquitination and degradation; IQGAP1 knockdown stabilizes TβRII, potentiating TGF-β1-induced myofibroblastic transdifferentiation. Co-IP, siRNA knockdown, ubiquitination assay, SMURF1 membrane targeting assay, in vivo tumor implantation in Iqgap1-deficient mice The Journal of clinical investigation High 23454766
2013 IQGAP1 WW domain peptide disrupts IQGAP1–ERK1/2 interactions and inhibits RAS- and RAF-driven tumorigenesis; disruption of IQGAP1 scaffold function bypasses acquired resistance to vemurafenib (BRAF inhibitor) and extends lifespan of tumor-bearing mice after systemic delivery. WW domain peptide competition, IQGAP1-null mouse tumorigenesis model, human tissue validation, in vivo survival studies Nature medicine High 23603816
2013 FOXO1 phosphorylated by AKT at Ser-319 binds IQGAP1 in the cytoplasm and impedes IQGAP1-dependent ERK1/2 phosphorylation, acting as a cytoplasmic tumor suppressor; this interaction is abolished by a non-phosphorylatable FOXO1 mutant. Co-IP, phospho-FOXO1 binding assays, siRNA, phosphomimetic/mutant FOXO1 constructs, pERK1/2 measurement in cells and patient specimens, in vivo peptide inhibitor studies The EMBO journal High 28279977
2014 IQGAP1 is identified as an LGR4-interacting protein that mediates RSPO-LGR4 interaction with the Wnt signalosome; RSPO stimulation enhances IQGAP1–DVL interaction, and IQGAP1 potentiates both canonical (via MEK1/2-mediated LRP5/6 phosphorylation) and non-canonical Wnt signaling through actin dynamics. Co-IP, pulldown, siRNA knockdown, Wnt reporter assay, LRP5/6 phosphorylation measurement Proceedings of the National Academy of Sciences of the United States of America Medium 24639526
2014 IQGAP1 binds directly to ERα and ERβ; the IQ domain of IQGAP1 and the hinge region of ERα mediate this interaction. Association is modulated by estradiol in cells. IQGAP1 knockdown attenuates estradiol-induced transcription of estrogen-responsive genes (pS2, progesterone receptor, cyclin D1). In vitro binding with purified proteins, Co-IP, domain-mapping constructs, siRNA knockdown, transcription reporter assay The Journal of biological chemistry High 24550401
2014 IQGAP1 interacts directly with PTPmu; amino acids 765–958 of PTPmu (juxtamembrane plus first phosphatase domain) mediate binding to IQGAP1. Constitutively active Cdc42 (and to a lesser extent Rac1) enhances this interaction. A peptide competing IQGAP1 binding to Rho GTPases blocks PTPmu-mediated neurite outgrowth. GST-fusion pulldown, direct binding assay, constitutively active Cdc42/Rac1 co-expression, competing peptide, neurite outgrowth assay The Journal of biological chemistry Medium 16380380
2014 IQGAP1 silencing abolishes HGF-induced endothelial barrier enhancement; IQGAP1 forms a complex with EB1 and cortactin in a Rac1-dependent manner, linking MT growth to peripheral cortactin and actin remodeling. IQGAP1ΔC (lacking C-terminal domain) attenuates cortactin–EB1 association. siRNA knockdown, Co-IP, confocal microscopy, transendothelial electrical resistance measurement, dominant-negative construct Molecular and cellular biology Medium 25022754
2013 IQGAP1 regulates β-catenin nuclear localization through importin-β5; depletion of IQGAP1 or importin-β5 in Xenopus embryos reduces Wnt-induced nuclear accumulation of β-catenin and expression of Wnt target genes. Ran GTPase contributes to IQGAP1/importin-β5-dependent β-catenin nuclear import. Morpholino knockdown in Xenopus embryos, nuclear fractionation, Wnt target gene expression assay, Co-IP The Journal of biological chemistry Medium 24196961
2015 IQGAP1 binds YAP directly via the IQ domain of IQGAP1 and the TEAD-binding domain of YAP; IQGAP1 knockout increases nuclear YAP-TEAD complex formation and YAP-TEAD-mediated transcription, establishing IQGAP1 as a negative regulator of Hippo/YAP transcriptional output. Co-IP in cells, in vitro binding with purified proteins, domain-mapping constructs, IQGAP1-null MEFs and CRISPR/Cas9 KD cells, TEAD transcription reporter assay The Journal of biological chemistry High 27440047
2015 IQGAP1 promotes CXCR4 expression and trafficking by regulating EEA-1+ endosome positioning relative to the MTOC; IQGAP1 depletion disrupts CXCR4 recycling, reduces SDF-1-induced ERK activation and cell migration. SDF-1 induces IQGAP1 binding to α-tubulin and localization to CXCR4-containing endosomes. siRNA knockdown, immunofluorescence, Co-IP, endosome positioning analysis, CXCR4 recycling assay, ERK activation assay, migration assay The Journal of cell biology High 26195666
2015 IQGAP1 forms dimers that stably bind actin filament sides and transiently cap barbed ends, organizing filaments into thin bundles, suppressing barbed end growth, and inhibiting filament disassembly. Different activities depend on distinct combinations of IQGAP1 domains and/or dimerization. Single-molecule and single-filament TIRF microscopy with purified full-length human IQGAP1, domain construct analysis Molecular biology of the cell High 34731043
2015 IQGAP1 controls tight junction formation through differential regulation of claudin recruitment (reducing claudin-2 expression and TJ recruitment while increasing claudin-4 recruitment); and through CDC42-JNK pathway: IQGAP1 KD increases CDC42 effector JNK activity, and dominant-negative CDC42 prevents the TER increase caused by IQGAP1 silencing. siRNA knockdown, TER measurement, quantitative confocal microscopy, biochemical claudin analysis, JNK activity assay, dominant-negative CDC42 Journal of cell science High 25588839
2017 The E3 ubiquitin ligase HECTD1 interacts with IQGAP1 and regulates its degradation through ubiquitination; loss of HECTD1 increases IQGAP1 levels, accelerates cell spreading and migration but impairs directionality. IQGAP1 overexpression phenocopies Hectd1-mutant cells; IQGAP1 knockdown rescues the migration defect. Hectd1 mutant MEFs, Co-IP, ubiquitination assay, siRNA, IQGAP1 overexpression rescue, cell migration and spreading assays Cell communication and signaling High 28073378
2017 IQGAP1 is SUMOylated by SUMO1 at K1445; this modification stabilizes IQGAP1 by reducing ubiquitination. IQGAP1 SUMOylation activates ERK, MEK, and AKT phosphorylation; the K1445R mutation abolishes these effects and reduces proliferation, migration, and tumor growth in vivo. SUMO1 co-expression, mutagenesis (K1445R), ubiquitination assay, kinase phosphorylation analysis, xenograft tumor model Cancer letters Medium 28987385
2017 IQGAP1 associates with IR (insulin receptor) via its IQ region binding the intracellular domain of IR, and with IRS-1 via the phosphotyrosine-binding domain of IRS-1 and the C-terminal tail of IQGAP1. In IQGAP1-null cells and mice, insulin-stimulated AKT and ERK phosphorylation, PI3K-IRS-1 association, and glucose homeostasis are significantly impaired. In vitro binding with purified proteins, Co-IP, IQGAP1-null mice, insulin signaling assays, glucose tolerance tests The Journal of biological chemistry High 28082684
2019 The kinase GLK/MAP4K3 directly phosphorylates IQGAP1 at Ser-480 via interaction through GLK proline-rich regions and the IQGAP1 WW domain, enhancing Cdc42 activation and cell migration; GLK-induced lung cancer metastasis is abolished by IQGAP1 depletion. Co-IP, in vitro kinase assay with purified proteins, phosphomimetic/phosphodeficient IQGAP1 constructs, Cdc42 activation assay, siRNA, GLK transgenic mice, lung metastasis model Cancer research High 31431460
2020 Ubiquitination of IQGAP1 at Lys-1155 and Lys-1230 in the GRD reduces its binding to CDC42 and RAC1; the non-ubiquitinatable GRD-2K mutant binds significantly more CDC42/RAC1, increases active CDC42 levels, and enhances cell migration compared to WT IQGAP1. MS-based ubiquitination site identification, K→R mutagenesis, GTPase pulldown, IQGAP1-null cell reconstitution, active CDC42/RAC1 assay, migration assay The Journal of biological chemistry High 32094223
2020 IQGAP1 sustains VEGFR2-mediated Rac1 activation in choroidal endothelial cells: IQGAP1-mediated Src activation initiates Rac1 activation; IQGAP1 binding to Rac1-GTP maintains sustained Rac1 activity necessary for endothelial cell migration and choroidal neovascularization. Iqgap1−/− mice have reduced laser-induced CNV volumes and decreased Rac1GTP and p-VEGFR2 in CNV lesions. Iqgap1-null mice, IQGAP1 Rac1-binding-deficient construct, siRNA, Rac1-GTP pulldown, p-VEGFR2 measurement, cell migration/tube formation assay Angiogenesis High 32783108
2020 IQGAP1 scaffolds AMPK by binding directly to the AMPK α1 subunit and to CaMKK2 (via the IQ domain); both associate with IQGAP1 in cells. IQGAP1 deletion reduces metformin- and Ca2+-stimulated AMPK activation; Ca2+-stimulated AMPK phosphorylation is rescued by IQGAP1 re-expression. IQGAP1-null mice show impaired transcriptional regulation of gluconeogenesis and fatty acid synthesis genes during fasting. In vitro binding with fusion proteins, Co-IP, siRNA KD, IQGAP1-null mice, reconstitution, AMPK kinase activity assay, gene expression analysis The Journal of biological chemistry High 33191271
2021 IQGAP1 scaffolds the Hippo pathway core kinases MST2 and LATS1, suppressing their kinase activity and YAP1-dependent transcription; IQGAP1 is a negative regulator of the non-canonical pro-apoptotic Hippo pathway. Bile acids regulate the IQGAP1–MST2–LATS1 module in hepatocellular carcinoma cells. Co-IP, kinase activity assays, YAP transcription reporter, IQGAP1 knockdown/overexpression, bile acid treatment Cells Medium 33672268
2014 IQGAP1 acts as a scaffold that colocalizes p190A-RhoGAP with RhoA to promote RhoA inactivation, thereby suppressing airway smooth muscle contractility. IQGAP1 knockdown or knockout increases RhoA activity and airway responsiveness; proximity ligation shows RhoA–p190A-RhoGAP colocalization is lost without IQGAP1. Co-IP, proximity ligation assay, Iqgap1-null mice, siRNA in human airway smooth muscle cells, RhoA activity assay, tracheal ring contractility, airway resistance measurement The Journal of clinical investigation High 25271629
2022 IQGAP1 bridges GSDMD to the ESCRT machinery (specifically Tsg101) to promote exosomal release of pro-IL-1β following NLRP3 inflammasome activation; this process requires LPS-induced GTP-bound CDC42 activation of IQGAP1. IQGAP1 was identified as a GSDMD-interacting protein by unbiased proteomics. Unbiased proteomics, Co-IP, siRNA knockdown, exosome isolation, NLRP3 inflammasome activation assay, CDC42 activation assay The EMBO journal High 36373462
2019 TRIM56 promotes K48-to-K63-linked poly-ubiquitination transition of IQGAP1 at Lys-1230 by interacting with it, which in turn promotes CDC42 activation to drive glioma cell migration and invasion. Co-IP, ubiquitination site mutagenesis, CDC42 activation assay, siRNA/overexpression, in vivo glioma model Cell death & disease Medium 36870986
2010 Rap1b colocalization with IQGAP1 upon NK cell activation facilitates sequential phosphorylation of B-Raf, C-Raf, and ERK1/2 and formation of a large IQGAP1-containing signalosome in the perinuclear region, required for NKG2D/Ly49D/NCR1-mediated cytokine production. Rap1a/b knockout mice, immunofluorescence co-localization, kinase phosphorylation analysis, cytokine assays The Journal of experimental medicine Medium 20733035
2017 IQGAP1 associates with the co-stimulatory receptor OX40 after OX40L ligation, mediated by the C-terminal IQGAP1 region, with TRAF2 bridging these two proteins. IQGAP1 deficiency enhances OX40 cosignaling (proliferation and cytokines) in CD4+ T cells; C-terminal IQGAP1 reconstitution restores normal responses. Co-IP, IQGAP1-null mice, IQGAP1 domain construct reconstitution, T-cell activation assays, EAE autoimmune model FASEB journal Medium 31914585
2017 IQGAP1 associates with NLRC3 and disrupts the NLRC3–STING interaction in the cytosol; IQGAP1 knockdown phenocopies NLRC3 deficiency, causing significantly more IFN-β production in response to cytosolic nucleic acids. Yeast two-hybrid (interaction identification), Co-IP in human epithelial cells, siRNA knockdown, IFN-β reporter assay Journal of immunology Medium 28864474
2023 ANGPTL4 (secreted by cancer-associated fibroblasts) binds IQGAP1 on the prostate cancer cell membrane, activating the Raf-MEK-ERK-PGC1α axis to promote mitochondrial biogenesis and OXPHOS metabolism, facilitating tumor growth and chemoresistance. GST pulldown, Co-IP, metabolomics, ELISA, siRNA/overexpression, drug screening, xenograft model Journal of advanced research Medium 39647634

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170. Cell 503 12110184
1996 IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs. The EMBO journal 342 8670801
2005 IQGAP1: a key regulator of adhesion and migration. Journal of cell science 304 15890984
2006 IQGAP1 in cellular signaling: bridging the GAP. Trends in cell biology 256 16595175
1997 IQGAP1, a Rac- and Cdc42-binding protein, directly binds and cross-links microfilaments. The Journal of cell biology 221 9199170
2005 IQGAP1 is a scaffold for mitogen-activated protein kinase signaling. Molecular and cellular biology 214 16135787
2011 IQGAP1 and its binding proteins control diverse biological functions. Cellular signalling 199 22182509
1999 IQGAP1 integrates Ca2+/calmodulin and Cdc42 signaling. The Journal of biological chemistry 192 9867866
2004 IQGAP1 binds ERK2 and modulates its activity. The Journal of biological chemistry 189 14970219
2003 IQGAP1 promotes cell motility and invasion. The Journal of biological chemistry 180 12900413
2013 IQGAP1 scaffold-kinase interaction blockade selectively targets RAS-MAP kinase-driven tumors. Nature medicine 163 23603816
2007 Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. The Journal of cell biology 162 17620407
2009 IQGAP1 regulation and roles in cancer. Cellular signalling 152 19269319
2006 IQGAP1 stimulates actin assembly through the N-WASP-Arp2/3 pathway. The Journal of biological chemistry 126 17085436
1999 IQGAP1 and calmodulin modulate E-cadherin function. The Journal of biological chemistry 126 10608854
2007 IQGAP1 stimulates proliferation and enhances tumorigenesis of human breast epithelial cells. The Journal of biological chemistry 116 17981797
2003 IQGAP1 as signal integrator: Ca2+, calmodulin, Cdc42 and the cytoskeleton. FEBS letters 115 12729888
2007 IQGAP1 regulates cell motility by linking growth factor signaling to actin assembly. Journal of cell science 108 17264147
2017 AKT-phosphorylated FOXO1 suppresses ERK activation and chemoresistance by disrupting IQGAP1-MAPK interaction. The EMBO journal 105 28279977
2014 RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America 97 24639526
2006 IQGAP1 mediates VE-cadherin-based cell-cell contacts and VEGF signaling at adherence junctions linked to angiogenesis. Arteriosclerosis, thrombosis, and vascular biology 93 16763158
2015 IQGAP1: insights into the function of a molecular puppeteer. Molecular immunology 83 25733387
2011 CLIP-170 and IQGAP1 cooperatively regulate dendrite morphology. The Journal of neuroscience : the official journal of the Society for Neuroscience 83 21430156
2005 IQGAP1 promotes neurite outgrowth in a phosphorylation-dependent manner. The Journal of biological chemistry 82 15695813
2011 MAPK scaffold IQGAP1 binds the EGF receptor and modulates its activation. The Journal of biological chemistry 80 21349850
2008 IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. PloS one 80 19050761
2013 IQGAP1 suppresses TβRII-mediated myofibroblastic activation and metastatic growth in liver. The Journal of clinical investigation 79 23454766
2023 IQGAP1 promotes mitochondrial damage and activation of the mtDNA sensor cGAS-STING pathway to induce endothelial cell pyroptosis leading to atherosclerosis. International immunopharmacology 78 37597406
2011 IQGAP1 regulates ERK1/2 and AKT signalling in the heart and sustains functional remodelling upon pressure overload. Cardiovascular research 75 21493702
2009 IQGAP1 regulates cell proliferation through a novel CDC42-mTOR pathway. Journal of cell science 71 19454477
2008 Combined analysis of Rac1, IQGAP1, Tiam1 and E-cadherin expression in gastric cancer. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 70 18246045
2010 IQGAP1 is overexpressed in hepatocellular carcinoma and promotes cell proliferation by Akt activation. Experimental & molecular medicine 67 20530982
2005 Self-association of IQGAP1: characterization and functional sequelae. The Journal of biological chemistry 65 16105843
2010 IQGAP1 and IQGAP2 are reciprocally altered in hepatocellular carcinoma. BMC gastroenterology 63 20977743
2020 IQGAP1 promotes anoikis resistance and metastasis through Rac1-dependent ROS accumulation and activation of Src/FAK signalling in hepatocellular carcinoma. British journal of cancer 62 32632148
2007 IQGAP1 binds Rap1 and modulates its activity. The Journal of biological chemistry 60 17517894
2007 The IQGAP1-Rac1 and IQGAP1-Cdc42 interactions: interfaces differ between the complexes. The Journal of biological chemistry 56 17984089
2012 Rho isoform-specific interaction with IQGAP1 promotes breast cancer cell proliferation and migration. The Journal of biological chemistry 55 22992742
2019 IQGAP1 promotes pancreatic cancer progression and epithelial-mesenchymal transition (EMT) through Wnt/β-catenin signaling. Scientific reports 54 31101875
1999 Cdc42, Rac1, and their effector IQGAP1 as molecular switches for cadherin-mediated cell-cell adhesion. Biochemical and biophysical research communications 52 10448058
2008 Cdc42 protein acts upstream of IQGAP1 and regulates cytokinesis in mouse oocytes and embryos. Developmental biology 50 18662680
2010 Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. The Journal of experimental medicine 46 20733035
2008 IQGAP1 integrates Ca2+/calmodulin and B-Raf signaling. The Journal of biological chemistry 45 18567582
2019 MAP4K3/GLK Promotes Lung Cancer Metastasis by Phosphorylating and Activating IQGAP1. Cancer research 44 31431460
2013 IQGAP1 protein regulates nuclear localization of β-catenin via importin-β5 protein in Wnt signaling. The Journal of biological chemistry 44 24196961
2017 SUMOylation of IQGAP1 promotes the development of colorectal cancer. Cancer letters 42 28987385
2021 Role of IQGAP1 in Carcinogenesis. Cancers 40 34439095
2016 IQGAP1 Binds to Yes-associated Protein (YAP) and Modulates Its Transcriptional Activity. The Journal of biological chemistry 39 27440047
2012 The cytoskeletal adaptor protein IQGAP1 regulates TCR-mediated signaling and filamentous actin dynamics. Journal of immunology (Baltimore, Md. : 1950) 39 22573807
2015 IQGAP1 is a phosphoinositide effector and kinase scaffold. Advances in biological regulation 38 26554303
2012 IQGAP1: a regulator of intracellular spacetime relativity. Journal of immunology (Baltimore, Md. : 1950) 38 22345702
2011 IQGAP1 protein binds human epidermal growth factor receptor 2 (HER2) and modulates trastuzumab resistance. The Journal of biological chemistry 38 21724847
2011 IQGAP1 in microbial pathogenesis: Targeting the actin cytoskeleton. FEBS letters 36 21295032
2022 The Ras GTPase-activating-like protein IQGAP1 bridges Gasdermin D to the ESCRT system to promote IL-1β release via exosomes. The EMBO journal 33 36373462
2015 miR-506 regulates breast cancer cell metastasis by targeting IQGAP1. International journal of oncology 33 26398880
2014 IQGAP1 binds to estrogen receptor-α and modulates its function. The Journal of biological chemistry 33 24550401
2014 IQGAP1 regulates endothelial barrier function via EB1-cortactin cross talk. Molecular and cellular biology 33 25022754
2013 A PAK6-IQGAP1 complex promotes disassembly of cell-cell adhesions. Cellular and molecular life sciences : CMLS 32 24352566
2007 Multiple proteins mediate IQGAP1-stimulated cell migration. Cellular signalling 32 17544257
2003 Identification of an IQGAP1/AKAP79 complex in beta-cells. Journal of cellular biochemistry 32 12938160
2014 Overexpression of DNAJB6 promotes colorectal cancer cell invasion through an IQGAP1/ERK-dependent signaling pathway. Molecular carcinogenesis 30 25044025
2010 An emerging role for IQGAP1 in regulating protein traffic. TheScientificWorldJournal 30 20495773
2015 IQGAP1 and IQGAP3 Serve Individually Essential Roles in Normal Epidermal Homeostasis and Tumor Progression. The Journal of investigative dermatology 29 25848980
2011 Protein 4.1R regulates cell migration and IQGAP1 recruitment to the leading edge. Journal of cell science 29 21750196
2022 Acinetobacter baumannii Outer Membrane Protein A Induces Pulmonary Epithelial Barrier Dysfunction and Bacterial Translocation Through The TLR2/IQGAP1 Axis. Frontiers in immunology 28 35844614
2012 IQGAP1 regulates hyaluronan-mediated fibroblast motility and proliferation. Cellular signalling 28 22634185
2020 IQGAP1 causes choroidal neovascularization by sustaining VEGFR2-mediated Rac1 activation. Angiogenesis 27 32783108
2021 The interplay between IQGAP1 and small GTPases in cancer metastasis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 26 33434854
2014 miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin. Oncology reports 26 25175832
2005 The receptor protein-tyrosine phosphatase PTPmu interacts with IQGAP1. The Journal of biological chemistry 26 16380380
2023 Integrin β4 promotes DNA damage-related drug resistance in triple-negative breast cancer via TNFAIP2/IQGAP1/RAC1. eLife 25 37787041
2021 MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation. Molecular oncology 25 33793053
2011 IQGAP1 involvement in MTOC and granule polarization in NK-cell cytotoxicity. European journal of immunology 25 21681737
2005 Expression pattern of the scaffold protein IQGAP1 in lung cancer. Oncology reports 25 15706411
2024 Cancer-associated fibroblasts regulate mitochondrial metabolism and inhibit chemosensitivity via ANGPTL4-IQGAP1 axis in prostate cancer. Journal of advanced research 24 39647634
2021 Single-molecule imaging of IQGAP1 regulating actin filament dynamics. Molecular biology of the cell 24 34731043
2016 An emerging role for IQGAP1 in tight junction control. Small GTPases 24 27880081
2011 IQGAP1 is a novel CXCR2-interacting protein and essential component of the "chemosynapse". PloS one 24 21876773
2023 ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor progression and chemotherapy resistance in gastric cancer. Cell death & disease 23 36792578
2023 Overexpressed ZC3H13 suppresses papillary thyroid carcinoma growth through m6A modification-mediated IQGAP1 degradation. Journal of the Formosan Medical Association = Taiwan yi zhi 22 36739231
2017 HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures. Cell communication and signaling : CCS 22 28073378
2017 The Scaffolding Protein IQGAP1 Interacts with NLRC3 and Inhibits Type I IFN Production. Journal of immunology (Baltimore, Md. : 1950) 22 28864474
2015 IQGAP1 controls tight junction formation through differential regulation of claudin recruitment. Journal of cell science 22 25588839
2021 CircRNA circ-IQGAP1 Knockdown Alleviates Interleukin-1β-Induced Osteoarthritis Progression via Targeting miR-671-5p/TCF4. Orthopaedic surgery 21 33675175
2019 A PI3K/AKT Scaffolding Protein, IQ Motif-Containing GTPase Associating Protein 1 (IQGAP1), Promotes Head and Neck Carcinogenesis. Clinical cancer research : an official journal of the American Association for Cancer Research 21 31597661
2017 New model for the interaction of IQGAP1 with CDC42 and RAC1. Small GTPases 21 28622072
2015 IQGAP1 promotes CXCR4 chemokine receptor function and trafficking via EEA-1+ endosomes. The Journal of cell biology 20 26195666
2014 Differential expression of IQGAP1/2 in Hepatocellular carcinoma and its relationship with clinical outcomes. Asian Pacific journal of cancer prevention : APJCP 20 24998570
2023 TRIM56 acts through the IQGAP1-CDC42 signaling axis to promote glioma cell migration and invasion. Cell death & disease 19 36870986
2017 Absence of IQGAP1 Protein Leads to Insulin Resistance. The Journal of biological chemistry 19 28082684
2016 IQGAP1 gene silencing induces apoptosis and decreases the invasive capacity of human hepatocellular carcinoma cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 19 27488117
2022 Targeting ARF1-IQGAP1 interaction to suppress colorectal cancer metastasis and vemurafenib resistance. Journal of advanced research 18 36396045
2020 Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42. The Journal of biological chemistry 18 32094223
2017 Knockdown of IQGAP1 inhibits proliferation and epithelial-mesenchymal transition by Wnt/β-catenin pathway in thyroid cancer. OncoTargets and therapy 18 28352188
2014 IQGAP1-dependent scaffold suppresses RhoA and inhibits airway smooth muscle contraction. The Journal of clinical investigation 18 25271629
2021 IQGAP1 Is a Scaffold of the Core Proteins of the Hippo Pathway and Negatively Regulates the Pro-Apoptotic Signal Mediated by This Pathway. Cells 17 33672268
2020 IQGAP1 binds AMPK and is required for maximum AMPK activation. The Journal of biological chemistry 17 33191271
2019 IQGAP1 restrains T-cell cosignaling mediated by OX40. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17 31914585
2017 IQGAP1‑siRNA inhibits proliferation and metastasis of U251 and U373 glioma cell lines. Molecular medicine reports 17 28259970
2005 Roles of IQGAP1 in cell polarization and migration. Novartis Foundation symposium 17 16355537

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