Affinage

MYL6

Myosin light polypeptide 6 · UniProt P60660

Round 2 corrected
Length
151 aa
Mass
16.9 kDa
Annotated
2026-04-29
84 papers in source corpus 16 papers cited in narrative 16 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MYL6 is a non-muscle essential light chain (ELC) that serves as a core structural and regulatory subunit of multiple myosin complexes and participates in diverse actin-based cellular processes including cell motility, platelet activation, spermatogenesis, and muscle differentiation. As an ELC of non-muscle myosin II (NM IIA/IIB), MYL6 associates with heavy chains MYH9 and MYH10 and regulatory light chains MYL12A/B and MYL9, with its stability dependent on co-expressed RLCs and further stabilized by the lncRNA MAFG-AS1 (PMID:21126233, PMID:33813778); it also functions as an ELC of myosin 5c, where it modulates ATPase activity and actin-based motility in a light-chain-composition-dependent manner (PMID:38606007). In platelets, MYL6 binds kindlin-3 and is required for integrin αIIbβ3 inside-out signaling, platelet aggregation, and hemostasis, as megakaryocyte-specific Myl6 knockout causes macrothrombocytopenia and thrombotic defects (PMID:38266679). MYL6 undergoes regulated cell-type-specific alternative splicing that alters C-terminal sequence and interaction properties across tissues, in breast cancer microenvironments, and under mechanical stress in podocytes (PMID:2304459, PMID:34515025, PMID:40418580).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1990 High

    Establishing MYL6 as the principal nonmuscle alkali myosin light chain resolved the identity of the ELC isoform used outside striated muscle and revealed that smooth-muscle and nonmuscle isoforms arise from alternative splicing at a single locus.

    Evidence cDNA cloning, Northern/Western blotting, and RT-PCR splicing analysis across species and tissues

    PMID:2304459

    Open questions at the time
    • Functional consequence of smooth-muscle-specific exon exclusion not determined
    • No biochemical assay of isoform-specific motor properties
  2. 1996 High

    Demonstrating that MYL6 has the lowest sarcomeric sorting specificity among ELC isoforms established that its N-terminal lobe lacks the determinants for myofibrillar targeting, consistent with a predominantly cytoplasmic/nonmuscle role.

    Evidence Double epitope-tagged chimeric isoform competition assay in cultured rat cardiomyocytes with confocal microscopy

    PMID:8856505

    Open questions at the time
    • Structural basis of N-terminal sorting determinants not resolved
    • Sorting behavior in non-cardiomyocyte cell types not tested
  3. 2006 Medium

    Discovery that MYL6 splicing patterns shift between breast cancer subtypes and between 2D and 3D culture (matching in vivo tumors) raised the possibility that MYL6 isoform balance is microenvironment-responsive and functionally relevant in cancer.

    Evidence Splicing-sensitive microarray with RT-PCR validation across MCF7 and MDA-MB-231 cells in 2D, 3D Matrigel, and xenograft models

    PMID:16488998

    Open questions at the time
    • Functional consequence of cancer-associated splice variants not tested
    • Splicing regulators acting on MYL6 in this context not identified
  4. 2011 High

    Proteomic identification of MYL6 as the ELC within NM IIA/IIB complexes, and demonstration that RLC knockdown destabilizes MYL6, established the subunit interdependence within the non-muscle myosin II holoenzyme.

    Evidence Co-immunoprecipitation of myosin II subunits and siRNA knockdown of MYL12A/B with quantitative Western blotting in NIH 3T3 fibroblasts

    PMID:21126233

    Open questions at the time
    • Whether MYL6 loss reciprocally destabilizes RLCs not tested
    • Stoichiometry of MYL6 within the complex not quantified
  5. 2015 Medium

    Identification of MYL6 in SPATA6-dependent myosin-based transport during spermiogenesis extended MYL6 function to male germ cell morphogenesis and head-tail coupling apparatus assembly.

    Evidence Spata6 knockout mouse, proteomic analysis, and co-immunoprecipitation of sperm myosin subunits

    PMID:25605924

    Open questions at the time
    • MYL6-specific loss-of-function in spermiogenesis not performed
    • Direct interaction between MYL6 and SPATA6 not demonstrated
  6. 2021 High

    Single-cell resolution mapping confirmed cell-type-specific MYL6 alternative splicing across >110,000 cells from 12 human tissues, establishing MYL6 as a paradigmatic example of ubiquitously expressed genes with regulated isoform diversity.

    Evidence SpliZ analysis of 10X Chromium scRNA-seq validated by RNA FISH, single-cell PCR, and Smart-seq2

    PMID:34515025

    Open questions at the time
    • Functional distinctions between cell-type-specific MYL6 isoforms remain uncharacterized
    • Splicing regulators responsible not identified
  7. 2021 Medium

    Showing that the lncRNA MAFG-AS1 directly binds MYL6, MYH9, and MYL12B and that its depletion disrupts NM IIA assembly and ATPase activity revealed an RNA-dependent mechanism for myosin complex stabilization.

    Evidence RNA immunoprecipitation, Co-IP of NM IIA subunits, ATPase assays, and siRNA knockdown

    PMID:33813778

    Open questions at the time
    • Whether MAFG-AS1 acts as a scaffold or allosteric regulator is unclear
    • Not independently replicated
  8. 2022 Medium

    Demonstration that anti-MYL6 antibodies from polyangiitis patients block G-to-F-actin polymerization and suppress NET formation established a functional role for MYL6 in neutrophil actin dynamics and innate immune effector function.

    Evidence Fluorescent actin polymerization assays and NET formation assays with anti-MYL6 antibody treatment in PMA-stimulated neutrophils, clinical cohort analysis

    PMID:36527167

    Open questions at the time
    • Mechanism by which MYL6 promotes actin polymerization not defined
    • Whether MYL6 loss-of-function (not just antibody blocking) phenocopies the effect is untested
  9. 2022 Medium

    Epistasis experiments placed MYL6 downstream of ADCK2 in melanoma cell motility control, linking MYL6 to a kinase-regulated migration pathway.

    Evidence MYL6 siRNA knockdown in ADCK2-overexpressing melanoma cells with scratch and transwell assays

    PMID:35205819

    Open questions at the time
    • Whether ADCK2 directly regulates MYL6 expression or acts through intermediaries unknown
    • Physical interaction between ADCK2 and MYL6 not demonstrated
  10. 2024 High

    In vitro reconstitution of myosin 5c with MYL6 as an ELC demonstrated that light chain composition directly modulates motor ATPase rate and gliding velocity, and that tropomyosin isoforms further tune these properties, providing a biochemical basis for MYL6-dependent cargo transport regulation.

    Evidence Recombinant Myo5c HMM reconstitution with varying CaM/Myl6/Myl12b combinations, ATPase and actin gliding assays

    PMID:38606007

    Open questions at the time
    • Cellular context in which Myo5c uses MYL6 versus CaM not defined
    • Structural basis of MYL6 modulation of Myo5c not resolved
  11. 2024 High

    Megakaryocyte-specific Myl6 knockout revealed an essential, non-redundant role for MYL6 in platelet integrin αIIbβ3 activation through a kindlin-3-dependent mechanism, causing macrothrombocytopenia and impaired hemostasis in vivo.

    Evidence Myl6fl/fl PF4-Cre conditional knockout mice, Co-IP of Myl6 with kindlin-3, flow cytometry, aggregation assays, in vivo thrombosis models

    PMID:38266679

    Open questions at the time
    • How MYL6 binding to kindlin-3 promotes integrin avidity at the structural level is unknown
    • Whether MYL6-kindlin-3 interaction is direct or bridged by myosin heavy chains not resolved
  12. 2024 High

    Identification of BAG5-HSPA8 as the chaperone system required for MYL6 folding during spermiogenesis provided the first mechanistic explanation for how MYL6 is correctly incorporated into myosin complexes during sperm assembly.

    Evidence BAG5 knockout mice, in vitro chaperone/folding assays, Co-IP of BAG5-HSPA8 complex, electron microscopy

    PMID:38454159

    Open questions at the time
    • Whether BAG5-HSPA8 chaperones MYL6 in somatic tissues or only in spermatids is unknown
    • Specific folding intermediates of MYL6 not characterized
  13. 2025 Medium

    Mechanical stretch of podocytes induces an MYL6 isoform switch with altered C-terminal sequence, linking biomechanical signaling to MYL6 splicing regulation in kidney glomerular cells.

    Evidence RNA-seq, quantitative proteomics, RT-PCR, and in situ hybridization in mechanically stretched podocytes

    PMID:40418580

    Open questions at the time
    • Functional consequence of the podocyte isoform switch on myosin complex function not tested
    • Mechano-responsive splicing regulators acting on MYL6 not identified
  14. 2025 Medium

    Discovery that MYL6 binds pseudouridine-modified RNA and is required for extracellular vesicle RNA secretion expanded its functional repertoire beyond actomyosin mechanics to RNA export, though this remains to be confirmed in peer review.

    Evidence (preprint) Genome-wide CRISPR screen, quantitative proteomics, pseudouridine-RNA binding assays, and EV RNA secretion assays in a neuronal cell line

    PMID:41280092

    Open questions at the time
    • Preprint; not yet peer-reviewed
    • Whether pseudouridine-binding is direct or mediated through an RNA-binding partner unclear
    • Mechanism linking MYL6 to vesicle RNA loading not defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of MYL6-kindlin-3 interaction in platelets, the functional consequences of cell-type-specific MYL6 splice isoforms, the identity of upstream splicing regulators, and whether MYL6's pseudouridine-RNA binding role operates through actomyosin-dependent vesicle trafficking or an independent mechanism.
  • No high-resolution structure of MYL6 in complex with kindlin-3 or NM IIA
  • Functional distinction between MYL6 splice isoforms not established by loss/gain-of-function for individual variants
  • Pseudouridine-binding mechanism and its relationship to myosin function uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005856 cytoskeleton 4 GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 1
Pathway
R-HSA-1474165 Reproduction 2 R-HSA-397014 Muscle contraction 2 R-HSA-109582 Hemostasis 1 R-HSA-168256 Immune System 1
Partners
BAG5FERMT3MYH10MYH9MYL12BMYL9PCBP1SPATA6
Complex memberships
Myosin 5c (Myo5c)Non-muscle myosin IIA (NM IIA)Non-muscle myosin IIB (NM IIB)

Evidence

Reading pass · 16 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 MYL6 (MLC3nm) is the major nonmuscle alkaline myosin light chain isoform expressed in fibroblasts and nonmuscle tissues. In smooth muscle, one exon is spliced out of the MLC3nm transcript to produce an alternative isoform, demonstrating isoform-specific alternative splicing at this locus. Phylogenetic analysis indicated MLC1sa arose by duplication of MLC3nm, and all alkali myosin light chain isoforms are likely functionally distinct. cDNA cloning, nucleotide sequencing, isoform-specific probes, Northern/Western blotting across species and tissues, alternative splicing analysis by RT-PCR Molecular and cellular biology High 2304459
1996 MYL6 (MLC3nm), the nonmuscle isoform of myosin alkali light chain, has the lowest sarcomeric sorting specificity among myosin light chain isoforms in cardiomyocytes, distributing throughout the cytoplasm rather than localizing to myofibril A-bands. The N-terminal lobe of each isoform is responsible for isoform-specific sarcomeric sorting. Sorting specificity increases in the developmental expression order: MLC3nm < MLC1sa < MLC1sb < MLC1f/MLC3f. Double epitope-tagging competition assay, co-expression of VSV- and mT-tagged isoforms in cultured rat cardiomyocytes, confocal microscopy, chimeric cDNA expression Journal of cell science High 8856505
2006 MYL6 undergoes cell-type-specific and culture-condition-dependent alternative splicing in breast cancer cells, with distinct splicing patterns detected between MCF7 and MDA-MB-231 cells and between 2D and 3D culture conditions. The MYL6 splicing pattern in 3D Matrigel culture resembles that seen in xenograft tumors in vivo. Splicing-sensitive oligonucleotide microarray, RT-PCR validation, 3D Matrigel culture, xenograft comparison Cancer research Medium 16488998
2011 MYL6 functions as an essential light chain (ELC) of non-muscle myosin II, forming a complex with the myosin heavy chains MYH9 (NMHC IIA) and MYH10 (NMHC IIB) and the regulatory light chains MYL12A, MYL12B, and MYL9 in NIH 3T3 fibroblasts. Knockdown of the regulatory light chains MYL12A/12B significantly reduces MYL6 protein levels, indicating that RLCs are required to maintain MYL6 stability within the myosin II complex. Proteomic co-immunoprecipitation, siRNA knockdown of RLCs, quantitative Western blotting, interaction analysis across cell and tissue types The Biochemical journal High 21126233
2015 SPATA6, a testis-specific protein required for sperm connecting piece assembly, interacts with MYL6 as part of myosin-based microfilament transport during spermiogenesis. Loss of SPATA6 disrupts sperm head-tail coupling apparatus (HTCA) formation, leading to acephalic spermatozoa, and proteomic analysis identified MYL6 among the myosin subunits involved in this transport process. Mouse Spata6 knockout, proteomic analysis of sperm, co-immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America Medium 25605924
2009 MYL6 physically interacts with the RNA-binding protein PCBP1 (a member of the hnRNP family), identified by yeast two-hybrid screening, pulldown in yeast, co-immunoprecipitation, and immunofluorescence microscopy, suggesting MYL6 participates in protein complexes linked to RNA metabolism pathways. Yeast two-hybrid, yeast pulldown, co-immunoprecipitation, immunofluorescent microscopy Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences Medium 19727228
2021 MYL6 undergoes cell-type-specific alternative splicing across human tissues at single-cell resolution, as demonstrated by SpliZ statistical analysis of >110,000 cells from 12 tissues. Cell-type-specific MYL6 splicing was validated independently by RNA FISH, single-cell PCR, and Smart-seq2, establishing that MYL6 splicing is regulated in a cell-type-specific manner among ubiquitously expressed genes. SpliZ statistical method applied to 10X Chromium single-cell RNA-seq, validation by RNA FISH, single-cell PCR, Smart-seq2 eLife High 34515025
2021 The lncRNA MAFG-AS1 directly interacts with three subunits of non-muscle myosin IIA (NM IIA): MYH9, MYL12B, and MYL6. Knockdown of MAFG-AS1 inhibits ATPase activity of MYH9 and disrupts the interaction among NM IIA subunits, indicating that MAFG-AS1 stabilizes the NM IIA complex (including MYL6) and is required for normal complex assembly and function. RNA immunoprecipitation, Co-IP of NM IIA subunits, ATPase activity assay, siRNA knockdown, cell proliferation/migration assays FASEB journal Medium 33813778
2022 Anti-MYL6 antibody (an anti-NET antibody found in microscopic polyangiitis patients) disrupts G-actin polymerization into F-actin and thereby suppresses PMA-induced neutrophil extracellular trap (NET) formation. This establishes that MYL6 plays a functional role in actin rearrangement required for NET formation in neutrophils. Fluorescent staining of actin dynamics (G-actin vs. F-actin), ELISA for anti-MYL6 antibody, NET formation assays with anti-MYL6 antibody treatment, clinical cohort analysis Arthritis research & therapy Medium 36527167
2022 ADCK2 regulates melanoma cell motility through MYL6: gene expression arrays showed ADCK2 expression correlates with MYL6 expression, and knockdown of MYL6 in ADCK2-overexpressing melanoma cells abrogates the inhibitory effect of ADCK2 on cell migration, placing MYL6 downstream of ADCK2 in a pathway controlling cell motility. siRNA knockdown, ADCK2 stable overexpression, scratch assay, transwell invasion assay, gene expression array Cancers Medium 35205819
2024 MYL6 is an essential light chain of myosin 5c (Myo5c). Functional reconstitution of Myo5c heavy meromyosin (HMM) with CaM alone or with CaM plus Myl6 and Myl12b showed that the light chain composition modulates Myo5c ATPase and motility activity. Tropomyosin isoforms (Tpm1.6, Tpm1.8, Tpm3.1) further alter Myo5c motor properties in the presence of Myl6/Myl12b. Recombinant protein purification, in vitro ATPase assay, actin gliding assay, co-reconstitution with different light chain combinations, immunohistochemical localization Frontiers in physiology High 38606007
2024 Myl6 is a novel binding partner of kindlin-3 in platelets and is required for integrin αIIbβ3 activation, platelet aggregation, proplatelet formation, and thrombotic function. Megakaryocyte-specific Myl6 knockout mice (Myl6fl/fl PF4-Cre) exhibit macrothrombocytopenia, impaired integrin αIIbβ3 inside-out signaling (particularly avidity modulation for multivalent ligands), defective platelet spreading, and impaired hemostasis/thrombosis in vivo. Conditional knockout mouse model (Myl6fl/fl PF4-Cre), Co-IP of Myl6 with kindlin-3, flow cytometry for integrin activation, platelet aggregation assays, in vivo hemostasis/thrombosis models Journal of thrombosis and haemostasis High 38266679
2024 BAG5, a co-chaperone expressed in spermatids, is required for proper folding of MYL6 (along with MYO5A and dynein proteins) during sperm head-tail coupling apparatus (HTCA) assembly. BAG5 forms a complex with HSPA8 and enhances HSPA8's substrate affinity, promoting folding of SPATA6 and cargo transport proteins including MYL6. BAG5 deficiency causes MYL6 misfolding, HTCA assembly failure, and acephalic spermatozoa. BAG5 knockout mice, in vivo and in vitro chaperone assays, Co-IP of BAG5-HSPA8 complex, protein folding assays, electron microscopy of sperm ultrastructure EMBO reports High 38454159
2025 MYL6 is a pseudouridine-binding protein required for secretion of RNAs via extracellular vesicles. Genome-wide CRISPR screening and proteomics identified MYL6 as a factor in RNA loading into extracellular vesicles. MYL6 binds pseudouridine-modified RNA, and its depletion impairs secretion of both synthetic pseudouridine-containing RNAs and endogenous RNAs into the extracellular space in a neuronal cell line. Genome-wide CRISPR screen, quantitative proteomics, high-sensitivity transcriptomics, pseudouridine-RNA binding assay, extracellular vesicle RNA secretion assays, MYL6 knockdown bioRxivpreprint Medium 41280092
2025 Mechanical stretch of podocytes induces an isoform switch in Myl6: two Myl6 isoforms show differential expression under mechanical stretch conditions, accompanied by a change in the C-terminal amino acid sequence, which is predicted to alter the interaction properties of the Myl6 protein within the myosin complex. RNA-seq with multiple alternative splicing analysis tools, quantitative proteomics, quantitative RT-PCR validation, in situ hybridization Journal of the American Society of Nephrology Medium 40418580
2026 The circRNA-encoded protein ARHGAP10-202aa physically interacts with MYL6 to promote myoblast differentiation in cattle skeletal muscle. In vivo overexpression of ARHGAP10-202aa enhances MYL6 expression and accelerates regeneration of injured tibialis anterior muscle in mice, placing MYL6 as a functional partner in ARHGAP10-202aa-mediated muscle development and regeneration. Co-immunoprecipitation of ARHGAP10-202aa with MYL6, in vitro translation, Western blotting with specific antibody, functional differentiation assays, in vivo muscle regeneration assay (tibialis anterior injury model) Journal of genetics and genomics Medium 41617081

Source papers

Stage 0 corpus · 84 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2015 Gene essentiality and synthetic lethality in haploid human cells. Science (New York, N.Y.) 657 26472760
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2007 Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proceedings of the National Academy of Sciences of the United States of America 360 17620599
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2003 Smooth muscle contraction and relaxation. Advances in physiology education 322 14627618
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2016 Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell reports 306 27342126
2020 The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. Nature 292 32322062
2009 Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 284 19190083
2019 Shared genetic and experimental links between obesity-related traits and asthma subtypes in UK Biobank. The Journal of allergy and clinical immunology 276 31669095
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
2022 EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3. Molecular cancer 257 35031058
2007 hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics 222 17207965
2010 MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis. Immunology and cell biology 221 20458337
2015 Spata6 is required for normal assembly of the sperm connecting piece and tight head-tail conjunction. Proceedings of the National Academy of Sciences of the United States of America 149 25605924
2023 Pan-cancer genetic analysis of disulfidptosis-related gene set. Cancer genetics 124 37879141
2011 Myosin regulatory light chains are required to maintain the stability of myosin II and cellular integrity. The Biochemical journal 104 21126233
2015 Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes. PloS one 94 25978043
2006 Cell type and culture condition-dependent alternative splicing in human breast cancer cells revealed by splicing-sensitive microarrays. Cancer research 71 16488998
1990 Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function. Molecular and cellular biology 56 2304459
2021 RNA splicing programs define tissue compartments and cell types at single-cell resolution. eLife 50 34515025
2007 Identification and expression analysis of genes associated with bovine blastocyst formation. BMC developmental biology 48 17559642
2020 The Progression of Acute Myeloid Leukemia from First Diagnosis to Chemoresistant Relapse: A Comparison of Proteomic and Phosphoproteomic Profiles. Cancers 40 32512867
2023 Identification of disulfidptosis-related genes and subgroups in Alzheimer's disease. Frontiers in aging neuroscience 35 37600517
1996 The intracompartmental sorting of myosin alkali light chain isoproteins reflects the sequence of developmental expression as determined by double epitope-tagging competition. Journal of cell science 27 8856505
2022 FAM83A is a potential biomarker for breast cancer initiation. Biomarker research 25 35183258
2020 Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle. Meat science 25 33248741
2021 HBx-upregulated MAFG-AS1 promotes cell proliferation and migration of hepatoma cells by enhancing MAFG expression and stabilizing nonmuscle myosin IIA. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24 33813778
2015 Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D. Chinese journal of natural medicines 18 26412427
2022 ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6. Cancers 17 35205819
2018 A Novel 12q13.2-q13.3 Microdeletion Syndrome With Combined Features of Diamond Blackfan Anemia, Pierre Robin Sequence and Klippel Feil Deformity. Frontiers in genetics 14 30524470
2024 BAG5 regulates HSPA8-mediated protein folding required for sperm head-tail coupling apparatus assembly. EMBO reports 13 38454159
2020 DNA Methylation Reorganization of Skeletal Muscle-Specific Genes in Response to Gestational Obesity. Frontiers in physiology 13 32848869
2003 Myosin light chain genes in the turkey (Meleagris gallopavo). Cytogenetic and genome research 13 14970726
2024 Unveiling the role of disulfidptosis-related genes in the pathogenesis of non-alcoholic fatty liver disease. Frontiers in immunology 11 38812509
1998 Characterisation of a bovine/murine hybrid cell panel informative for all bovine autosomes. Animal genetics 11 9745669
2022 Transcriptome analyses of potential regulators of pre- and post-ovulatory follicles in the pigeon (Columba livia). Reproduction, fertility, and development 10 35366957
2007 Hedgehog-independent overexpression of transforming growth factor-beta1 in rhabdomyosarcoma of Patched1 mutant mice. International journal of oncology 10 17611698
2021 miRNAs and Their Gene Targets-A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia. Diagnostics (Basel, Switzerland) 8 33923995
2024 Targeting the High-Density Lipoprotein Proteome for the Treatment of Post-Acute Sequelae of SARS-CoV-2. International journal of molecular sciences 7 38674105
2024 Identification and verification of disulfidptosis-related genes in sepsis-induced acute lung injury. Frontiers in medicine 7 39262873
2022 Low disease activity of microscopic polyangiitis in patients with anti-myosin light chain 6 antibody that disrupts actin rearrangement necessary for neutrophil extracellular trap formation. Arthritis research & therapy 7 36527167
2021 RBBP4 Enhances Platinum Chemo Resistance in Lung Adenocarcinoma. BioMed research international 6 33506032
2018 Duplication of a Single myhz1.1 Gene Facilitated the Ability of Goldfish (Carassius auratus) to Alter Fast Muscle Contractile Properties With Seasonal Temperature Change. Frontiers in physiology 6 30568597
2009 Identification of novel partner proteins of PCBP1. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 6 19727228
2024 Pan-cancer and single-cell analysis of actin cytoskeleton genes related to disulfidptosis. Open medicine (Warsaw, Poland) 5 38584831
2024 Disulfidptosis: A New Target for Parkinson's Disease and Cancer. Current issues in molecular biology 5 39329952
2023 A comprehensive landscape of transcription profiles and data resources for human leukemia. Blood advances 5 36595475
2012 Novel transcripts and alternatively spliced genes are associated with early development in bovine embryos. Animal : an international journal of animal bioscience 5 23031207
2025 Alternative Splicing in Mechanically Stretched Podocytes as a Model of Glomerular Hypertension. Journal of the American Society of Nephrology : JASN 4 40418580
2024 Myosin light chain 6 (Myl6) interacts with kindlin-3 and is required to support integrin αIIbβ3 activation in platelets in mice. Journal of thrombosis and haemostasis : JTH 4 38266679
2024 Novel insights of disulfidptosis-mediated immune microenvironment regulation in atherosclerosis based on bioinformatics analyses. Scientific reports 4 39521794
2024 Motor properties of Myosin 5c are modulated by tropomyosin isoforms and inhibited by pentabromopseudilin. Frontiers in physiology 3 38606007
2024 Evaluation of a proteomic signature coupled with the kidney failure risk equation in predicting end stage kidney disease in a chronic kidney disease cohort. Clinical proteomics 3 38762513
2024 Characterization and proteomic analysis of plasma-derived small extracellular vesicles in locally advanced rectal cancer patients. Cellular oncology (Dordrecht, Netherlands) 3 39162991
2023 Analysis of RNA processing directly from spatial transcriptomics data reveals previously unknown regulation. bioRxiv : the preprint server for biology 3 36993757
2025 A Novel Disulfidptosis-Related Diagnostic Gene Signature and Differential Expression Validation in Ischaemic Cardiomyopathy. Journal of cellular and molecular medicine 2 40070032
2021 Proteomic and electron microscopy study of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells in three-dimensional culture. Proteomics 2 34674377
2026 Circulating Extracellular Vesicle Protein Biomarkers for the Early Detection of High-Grade Serous Ovarian Cancer. Molecular & cellular proteomics : MCP 0 41519436
2026 The ARHGAP10-202aa protein encoded by circARHGAP10 promotes skeletal muscle development and regeneration. Journal of genetics and genomics = Yi chuan xue bao 0 41617081
2026 Single-cell sequencing combined with transcriptome sequencing to reveal the molecular mechanisms related to integrated stress responses in atherosclerosis. Journal of cardiothoracic surgery 0 41840716
2026 Shared splicing dysregulation in heart failure associated with dilated and ischemic cardiomyopathy and spatial specificity across cardiac regions. Cardiovascular research 0 41877661
2025 Identification of disulfidptosis-related genes and subgroups in spinal cord injury. Spinal cord 0 40319145
2025 Sepsis Important Genes Identification Through Biologically Informed Deep Learning and Transcriptomic Analysis. Clinical and experimental pharmacology & physiology 0 40356040
2025 Investigating the role of disulfidoptosis in spinal cord injury and development of a novel diagnostic model. Acta orthopaedica et traumatologica turcica 0 40726387
2025 Pseudouridine selects RNAs for extracellular transport. bioRxiv : the preprint server for biology 0 41280092
2025 Identification of disulfidptosis-related genes in immunity and immunotherapy in diabetic foot ulcer. Annals of medicine and surgery (2012) 0 41675755