Affinage

PCBP1

Poly(rC)-binding protein 1 · UniProt Q15365

Length
356 aa
Mass
37.5 kDa
Annotated
2026-06-10
100 papers in source corpus 48 papers cited in narrative 48 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PCBP1 (hnRNP E1) is a multifunctional poly(C)-binding KH-domain protein that operates at the interface of post-transcriptional gene regulation and cytosolic iron homeostasis (PMID:9160751, PMID:22055506). As a translational silencer, it binds C-rich cis-elements such as the dimeric DICE in the 15-lipoxygenase 3' UTR and blocks 80S ribosome assembly, an activity reconstituted from recombinant protein and requiring two PCBP1 molecules per dimeric element (PMID:9160751, PMID:11827469); it extends this silencing to TGF-β-regulated EMT transcripts (Dab2, ILEI), PRL-3, Cdc27, and ZEB2 through its KH domains binding to defined 3'- or 5'-UTR consensus motifs (PMID:20154680, PMID:20609352, PMID:27102006, PMID:27067543, PMID:41117067). The same RNA-binding activity is repurposed for mRNA stabilization, where PCBP1 binds C/U-rich elements to protect transcripts including eNOS, mu-opioid receptor, p27Kip1, and GM-CSF (Csf2) (PMID:23478261, PMID:27836661, PMID:30086790, PMID:29958803). PCBP1 also functions as a nuclear alternative splicing regulator, negatively controlling CD44 variant exon inclusion and STAT3 exon 23 in concert with partners SMAD3, THAP11, TIA1/RBM39, and modulating FosB splicing in neurons (PMID:27746021, PMID:22673507, PMID:28193846, PMID:31223278, PMID:35730718). Independently of RNA, PCBP1 is a cytosolic iron chaperone that binds Fe(II) via cysteine/glutamate residues and a bound glutathione and delivers it to ferritin, PHD/FIH hydroxylases, deoxyhypusine hydroxylase, and the BolA2-Glrx3 [2Fe-2S] assembly pathway (PMID:22055506, PMID:24843120, PMID:28375153, PMID:31406370). Its activities are gated by phosphorylation (Akt2 at Ser43; PAK1 at Thr60/Thr127) that releases it from target RNAs, by homocysteinylation that unmasks RNA binding during folate deficiency, by O-GlcNAcylation at Thr99, and by ARIH1-mediated ubiquitin degradation, while iron itself protects PCBP1 from caspase cleavage (PMID:17389360, PMID:20154680, PMID:21930702, PMID:41117067, PMID:35102251, PMID:29958803). PCBP1 is essential for early mouse embryogenesis, erythroid iron loading and hemoglobinization, and T-cell cytokine programs that drive autoimmunity (PMID:26527618, PMID:28375153, PMID:29958803).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1997 High

    Established PCBP1's founding mechanism: how a 3' UTR element can repress translation, by showing hnRNP E1 binds the LOX DICE and blocks 80S ribosome assembly.

    Evidence Protein purification from reticulocytes with reconstituted cell-free translation and DICE-reporter transfection

    PMID:9160751

    Open questions at the time
    • Did not define the structural basis of 80S inhibition
    • Generality beyond LOX not yet established
  2. 2002 Medium

    Refined the silencing mechanism by showing stoichiometry matters — a dimeric DICE binding two PCBP1 molecules is required for repression, decoupling binding from inhibition.

    Evidence EMSA, sucrose gradients, and in vitro translation with DICE repeat-variant reporters

    PMID:11827469

    Open questions at the time
    • Structural model of the dimeric complex not resolved
    • single lab in vitro
  3. 2006 High

    Showed PCBP1 silencing is recruited combinatorially via protein partners, being delivered to A2RE RNA through direct interaction with hnRNP A2.

    Evidence Yeast two-hybrid, reciprocal co-IP, FCS, in vitro translation and microinjection

    PMID:16775011

    Open questions at the time
    • In vivo target mRNAs of the A2/E1 complex not enumerated
  4. 2007 High

    Identified the first switch controlling PCBP1 — PAK1 phosphorylation at Thr60/Thr127 releases it from DICE to reverse silencing and redirect it to transcription and CD44 splicing.

    Evidence Y2H, kinase and luciferase assays, polysome fractionation, ChIP, and splicing minigene assays

    PMID:17389360

    Open questions at the time
    • Direct physiological PAK1 substrate sites in vivo not fully mapped
    • single lab
  5. 2010 High

    Connected PCBP1 to a signaling pathway and disease program: Akt2 phosphorylation at Ser43 releases it from the BAT element to derepress EMT transcripts, linking TGF-β to translational control.

    Evidence Polysome profiling, RNA-protein binding, Ser43Ala mutagenesis and translational reporters

    PMID:20154680 PMID:20361869 PMID:20609352

    Open questions at the time
    • Full set of BAT-element targets incompletely defined
    • relationship between translational and splicing roles unclear
  6. 2011 High

    Revealed an entirely separate molecular role — PCBP1 as a cytosolic iron chaperone metallating PHD/FIH hydroxylases, coupling iron availability to HIF1α regulation.

    Evidence siRNA depletion, reconstituted in vitro iron delivery with purified Fe-PCBP1, co-IP and hydroxylation/degradation assays

    PMID:22055506

    Open questions at the time
    • Full client enzyme repertoire not yet defined
    • structural basis of Fe handoff unresolved
  7. 2011 High

    Demonstrated that covalent modification can switch on RNA binding — homocysteinylation of KH-domain cysteines unmasks an RNA pocket, making PCBP1 a folate-deficiency sensor.

    Evidence In vitro RNA-protein binding with purified components, in vitro translation, covalent modification analysis

    PMID:21930702

    Open questions at the time
    • In vivo physiological extent of homocysteinylation not quantified
  8. 2013 High

    Established the mRNA-stabilizing arm of PCBP1 and its phospho-regulation, showing it binds C/U-rich 3' UTRs to stabilize eNOS mRNA, an interaction disrupted by Akt/Ser43 phosphorylation under hypoxia.

    Evidence RIP, EMSA, mRNA half-life and phosphorylation/fractionation assays

    PMID:23478261 PMID:27836661

    Open questions at the time
    • Determinants distinguishing stabilizing vs silencing outcomes on C-rich elements unresolved
  9. 2014 High

    Generalized the iron-chaperone function to multiple cytosolic non-heme iron enzymes (DOHH, aconitase) in an iron-dependent, interaction-based handoff.

    Evidence siRNA depletion, in vitro iron delivery with lysates, iron-dependent co-IP, and DOHH/aconitase activity assays

    PMID:24843120

    Open questions at the time
    • Mechanism of client selectivity not defined
  10. 2015 High

    Demonstrated PCBP1 is individually essential and non-redundant with PCBP2 through peri-implantation embryonic lethality of the null mouse.

    Evidence Pcbp1 germline knockout mouse phenotyping

    PMID:26527618

    Open questions at the time
    • Which molecular function underlies lethality not isolated
  11. 2016 High

    Mapped the splicing-regulatory mechanism: phospho-SMAD3 partners with PCBP1 in nuclear speckles to inhibit spliceosome assembly on CD44, and defined the KH-domain consensus disrupted by Ser43 phosphorylation.

    Evidence Reciprocal co-IP, RIP, colocalization, phospho-mutant epistasis, binding kinetics, and cell-cycle phenotyping

    PMID:27067543 PMID:27102006 PMID:27746021

    Open questions at the time
    • Genome-wide splicing target set incomplete
    • structural model of the PCBP1-spliceosome interface lacking
  12. 2017 High

    Defined PCBP1's role in erythropoiesis as a ferritin iron donor whose iron-dependent protein interaction supports heme synthesis, with knockout mice showing microcytic anemia.

    Evidence Pcbp1 knockout mouse, ex vivo erythroid differentiation, ferritin iron-flux and in vitro protein-interaction assays

    PMID:28375153 PMID:29032941

    Open questions at the time
    • Coordination with NCOA4-mediated ferritinophagy not fully mechanistically integrated
  13. 2017 Medium

    Expanded the regulatory repertoire to miRNA processing, splicing co-factor complexes, and an autoregulatory loop, broadening PCBP1's reach across RNA metabolism.

    Evidence AGO2 co-IP and hypomorphic mouse (miRNA); TIA1/RBM39 complex co-IP and spliceosome assays; in vitro RNA binding for autoregulation

    PMID:28077174 PMID:28193846 PMID:28250194 PMID:28381556

    Open questions at the time
    • Direct biochemical role within the miRNA machinery not reconstituted
    • single-lab findings
  14. 2018 High

    Linked iron status to immune and apoptotic outcomes: iron protects PCBP1 from caspase cleavage to sustain Csf2 mRNA stability, and oxidized-RNA sensing via two 8-oxoG residues drives apoptosis.

    Evidence T-cell-specific knockout with EAE, mRNA stability and caspase assays; CRISPR KO with oxidized-RNA binding and caspase/PARP readouts

    PMID:29891675 PMID:29958803 PMID:30086790

    Open questions at the time
    • How a single protein integrates iron, RNA oxidation and apoptosis mechanistically not unified
  15. 2019 High

    Provided the structural-biochemical basis of iron chaperoning, showing Fe(II) coordination by Cys/Glu plus glutathione and handoff to BolA2 for [2Fe-2S] assembly, linking ferrous-iron and Fe-S systems.

    Evidence Interactome proteomics, in vitro reconstitution of Fe-GSH-PCBP1-BolA2 complex, mutagenesis of coordinating residues, cluster-assembly assays

    PMID:31223278 PMID:31406370

    Open questions at the time
    • Atomic structure of the metallated complex not resolved
    • in-cell flux through the pathway not quantified
  16. 2020 High

    Revealed paralog-specific and innate-immune functions — PCBP1 vs PCBP2 produce opposing apoptotic outcomes on 8-oxoG-RNA, and PCBP1 directly binds DNA to promote cGAS activation.

    Evidence CRISPR KO of both paralogs with rescue; biochemical purification, DNA-binding and cGAS activation assays; checkpoint/Treg phenotyping

    PMID:32067958 PMID:32415261 PMID:32440711 PMID:32523987 PMID:32647012

    Open questions at the time
    • Structural basis for opposite PCBP1/PCBP2 effects unknown
    • mechanism of DNA-dependent cGAS enhancement undefined
  17. 2022 Medium

    Identified upstream control and new effector roles: ARIH1 ubiquitinates PCBP1 to drive EMT, PCBP1 adapts NAT10 for mRNA ac4C modification, and binds poly(C) DNA recruiting DHX9 to resolve R-loops.

    Evidence Y2H/co-IP/ubiquitination (ARIH1); complex co-IP and ac4C mapping (NAT10/TDP43); ChIP-seq, helicase co-IP and R-loop assays; FosB splicing conditional KO

    PMID:35102251 PMID:35730718 PMID:39342995 PMID:39556689

    Open questions at the time
    • Direct DHX9-binding interface unmapped
    • physiological scope of ac4C adapter role unclear
    • single-lab studies
  18. 2023 Medium

    Extended PCBP1 mRNA-stability control across cancer contexts (LACTB, TPM3, DKK1, CCL2), tying it to ferroptosis, Wnt signaling, and inflammation.

    Evidence RIP, RNA pulldown, luciferase, and mRNA-stability assays with conditional knockout for CCL2/colitis

    PMID:35287546 PMID:35907982 PMID:36702545 PMID:37157950

    Open questions at the time
    • Binding-site consensus across these targets not unified
    • several are low-confidence single-assay studies
  19. 2025 Medium

    Added a metabolic PTM switch — OGT-mediated O-GlcNAcylation at Thr99 disrupts PCBP1 binding to ZEB2 mRNA to derepress translation and drive metastasis.

    Evidence T99 site mutagenesis, RIP, in vitro OGT modification, polysome profiling, and in vivo metastasis models

    PMID:41117067

    Open questions at the time
    • Interplay between O-GlcNAcylation and phosphorylation switches not resolved
    • single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PCBP1 partitions among its iron-chaperone, translational-silencing, mRNA-stabilizing, splicing, and DNA-binding activities within a single cell, and what structural states underlie each, remains unresolved.
  • No unifying structural model of how one KH-domain protein switches between RNA, DNA and iron-binding modes
  • Quantitative apportionment of cellular PCBP1 among functions unknown
  • Cross-talk among phosphorylation, homocysteinylation, O-GlcNAcylation and ubiquitination not integrated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 7 GO:0060090 molecular adaptor activity 4 GO:0140104 molecular carrier activity 4 GO:0045182 translation regulator activity 3 GO:0003677 DNA binding 2 GO:0140299 molecular sensor activity 2 GO:0008289 lipid binding 1
Localization
GO:0005634 nucleus 4 GO:0005829 cytosol 4 GO:0005654 nucleoplasm 2
Pathway
R-HSA-1430728 Metabolism 5 R-HSA-168256 Immune System 5 R-HSA-8953854 Metabolism of RNA 5 R-HSA-1266738 Developmental Biology 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-74160 Gene expression (Transcription) 2
Partners
AGO2ARIH1BOLA2DHX9NAT10PCBP2SMAD3TIA1

Evidence

Reading pass · 48 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 hnRNP E1 (PCBP1) and hnRNP K purified from rabbit reticulocytes specifically bind the differentiation control element (DICE) in the 3' UTR of 15-lipoxygenase (LOX) mRNA and silence its translation by specifically inhibiting 80S ribosome assembly; this was reconstituted in a cell-free translation system with recombinant proteins, and both cap-dependent and IRES-mediated translation are inhibited. Protein purification from reticulocytes, reconstituted cell-free translation system, transfection of HeLa cells with DICE-reporter, co-immunoprecipitation of silenced mRNA Cell High 9160751
2002 Translational silencing of LOX mRNA by hnRNP E1 requires binding of at least two hnRNP E1 molecules to a dimeric (2R) DICE configuration; a single repeat is insufficient for translational inhibition even though E1 binding still occurs. Electrophoretic mobility shift assay (EMSA), sucrose gradient centrifugation, in vitro translation with DICE repeat-variant reporter mRNAs Journal of molecular biology Medium 11827469
2006 hnRNP E1 (PCBP1) directly binds hnRNP A2 via protein-protein interaction and is recruited to A2 response element (A2RE) RNA in an hnRNP A2-dependent manner; overexpression or microinjection of hnRNP E1 inhibits translation of A2RE mRNA (but not non-A2RE RNA) both in vivo and in a cell-free translation system, in an hnRNP A2-dependent manner. Yeast two-hybrid, reciprocal co-immunoprecipitation, in vitro cross-linking, fluorescence correlation spectroscopy, in vitro translation system, microinjection Molecular biology of the cell High 16775011
2007 PAK1 phosphorylates PCBP1 on Thr60 and Thr127 in response to mitogenic stimulation; this phosphorylation releases PCBP1 from DICE elements, reversing translational inhibition of DICE-containing mRNAs (including endogenous L1-CAM). PAK1 activation also promotes nuclear retention of PCBP1, recruitment to the eIF4E promoter to stimulate eIF4E transcription, and alternative splicing of CD44 via PCBP1 interaction with Caper alpha (a U2 snRNP auxiliary factor). Yeast two-hybrid, phosphorylation assays, luciferase reporter, polysome fractionation, ChIP, RNA splicing minigene assay, co-immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America High 17389360
2010 TGF-β induces phosphorylation of hnRNP E1 (PCBP1) at Ser43 by protein kinase Bβ/Akt2, causing its release from the BAT element in the 3' UTR of Dab2 and ILEI mRNAs, thereby relieving translational silencing of these EMT-associated transcripts and inducing EMT. Polysome profiling, RNA-protein binding assays, phosphorylation assays, knockdown/overexpression with translational reporter assays, site-directed mutagenesis (Ser43Ala) Nature cell biology High 20154680
2010 PCBP1 binds GCCCAG motifs in the 5' UTR of PRL-3 mRNA and retards incorporation of PRL-3 mRNA into polyribosomes, suppressing its translation; overexpression of PCBP1 inhibits PRL-3 and inactivates AKT, while knockdown of PCBP1 increases PRL-3 protein levels. Polyribosome/polysome fractionation, RNA immunoprecipitation, reporter assay, overexpression/knockdown Cancer cell Medium 20609352
2010 PCBP1 negatively regulates alternative splicing of CD44 variant exons (v3, v5, v6, v8, v10) in HepG2 hepatoma cells; enforced PCBP1 expression inhibits CD44 variant expression and invasion, while knockdown induces variant splicing. Overexpression/knockdown, RT-PCR for CD44 variants, invasion assay Molecular cancer Medium 20361869
2011 PCBP1 is an iron chaperone that binds ferrous iron and delivers it to HIF prolyl hydroxylases (PHD1, PHD2, PHD3) and asparaginyl hydroxylase (FIH1) for metallation; depletion of PCBP1 or PCBP2 leads to loss of PHD activity, reduced prolyl hydroxylation of HIF1α, impaired VHL/proteasome degradation of HIF1α, and accumulation of active HIF1. PHD activity was restored in vitro by addition of purified Fe-PCBP1, and PCBP1 bound to PHD2 and FIH1 in vivo. siRNA depletion, in vitro iron delivery reconstitution with purified Fe-PCBP1, co-immunoprecipitation, prolyl hydroxylation assays, HIF1α degradation assays Cell metabolism High 22055506
2011 PCBP1 mediates constitutive (housekeeping) degradation of MAVS (mitochondrial antiviral signaling adaptor) in uninfected cells, using the same mechanism as PCBP2; overexpression of PCBP1 impairs MAVS-mediated antiviral responses through PCBP1-induced MAVS degradation, while knockdown of PCBP1 increases MAVS levels and antiviral signaling. Overexpression/knockdown, antiviral response assays, subcellular fractionation, western blot for MAVS protein levels Cell research Medium 22105485
2011 hnRNP E1 (PCBP1) acts as a physiological sensor of folate deficiency: intracellular accumulation of L-homocysteine (during folate deficiency) induces covalent binding of homocysteine to cysteine residues in the KH domains of hnRNP E1 via mixed disulfide bonds, unmasking an RNA-binding pocket and enabling high-affinity interaction with the folate receptor-α mRNA cis-element, thereby stimulating folate receptor translation. In vitro RNA-protein binding assays with purified components, in vitro translation, siRNA knockdown, covalent modification analysis The Journal of biological chemistry High 21930702
2012 THAP11 is a binding partner of PCBP1; THAP11 overexpression inhibits CD44 v6 expression and cell invasion in HepG2 cells, but fails to do so when the PCBP1-binding domain of THAP11 is deleted or when PCBP1 is knocked down, indicating that THAP11 regulates CD44 alternative splicing through interaction with PCBP1. Co-immunoprecipitation, deletion mutant analysis, overexpression/knockdown, CD44 variant expression assay, invasion assay FEBS letters Medium 22673507
2012 PCBP1 is required for maintenance of the transcriptionally silent state in fully grown mouse oocytes; siRNA-mediated knockdown of Pcbp1 in GV oocytes disrupts the NSN-to-SN nucleolus ratio, reactivates transcription (detected by BrUTP incorporation and active RNA Pol II/H3K4me3 markers), and upregulates ~4,000 transcripts. siRNA microinjection into oocytes, BrUTP incorporation assay, immunofluorescence for phospho-RNA Pol II CTD and H3K4me3, microarray transcriptome analysis Cell cycle Medium 22801551
2013 hnRNP E1 (PCBP1) binds pyrimidine-rich (C/CU-rich) elements in the 3' UTR of eNOS mRNA and stabilizes it; knockdown of hnRNP E1 decreases eNOS mRNA half-life, mRNA levels, and protein expression. Under hypoxia, increased Akt-mediated serine phosphorylation (including Ser43) of hnRNP E1 and increased nuclear localization disrupt hnRNP E1/eNOS 3'-UTR interactions, decreasing eNOS mRNA stability. The hnRNP E1/eNOS 3'-UTR complex also protects eNOS mRNA from inhibition by antisense RNA (sONE) and miR-765. RNA immunoprecipitation, EMSA, siRNA knockdown, mRNA half-life assay, phosphorylation analysis, nuclear/cytoplasmic fractionation Molecular and cellular biology High 23478261
2014 PCBP1 and PCBP2 deliver iron to deoxyhypusine hydroxylase (DOHH), a dinuclear iron enzyme; cells depleted of PCBP1 or PCBP2 exhibit loss of DOHH activity and loss of the holo form of the enzyme. Lysates containing PCBP1/2 converted apo-DOHH to holo-DOHH in vitro more efficiently than lysates lacking them. PCBP1 bound to DOHH in iron-treated cells but not in iron-deficient cells. Depletion of PCBP1/2 also led to loss of cytosolic aconitase activity, suggesting a broader role in iron delivery to cytosolic non-heme iron enzymes. siRNA depletion, in vitro iron delivery assay with cell lysates, co-immunoprecipitation, enzyme activity assays (DOHH, aconitase) Proceedings of the National Academy of Sciences of the United States of America High 24843120
2015 Pcbp1-null mouse embryos are non-viable at the peri-implantation stage, demonstrating that PCBP1 is individually essential for early mouse embryonic development and has evolved a non-redundant function distinct from its paralog PCBP2. Pcbp1 germline knockout mouse, embryonic lethality phenotyping Molecular and cellular biology High 26527618
2016 PCBP1/hnRNP E1 translationally silences Cdc27 mRNA in normal mammary epithelial cells; shRNA-mediated knockdown of hnRNP E1 abrogates this silencing, causing constitutive CDC27 expression, premature activation of G2-M-APC/C-CDC20, aberrant degradation of FZR1/CDH1, loss of APC/C-CDH1 activity, mitotic aberrations, and aneuploidy. shRNA knockdown, polysome profiling, western blot, cell cycle analysis, mitotic aberration assays Molecular cancer research Medium 27102006
2016 TGF-β directly regulates alternative splicing of CD44 through phosphorylated SMAD3 (pT179) interaction with PCBP1; SMAD3 and PCBP1 colocalize in SC35-positive nuclear speckles and interact at the variable exon region of CD44 pre-mRNA to inhibit spliceosome assembly, favoring expression of mesenchymal CD44s over epithelial CD44E isoform. Co-immunoprecipitation, immunofluorescence/colocalization, splicing reporter assays, RIP, SMAD3 phospho-mutant analysis Molecular cell High 27746021
2016 hnRNP E1 (PCBP1) KH domains mediate binding to a defined consensus motif in the 3' UTR of target mRNAs; phosphorylation at Ser43 (p-hnRNP E1 by Akt2) disrupts KH domain-RNA interaction, correlating with high p-hnRNP E1 levels in metastatic cells. An hnRNP E1 consensus binding motif was biochemically characterized for binding kinetics with KH domains. RNA-binding kinetics assays, KH domain deletion/mutation analysis, phospho-specific antibodies, western blot in cellular progression models Nucleic acids research Medium 27067543
2016 PCBP1 stabilizes mu-opioid receptor (MOR) mRNA by binding a C-rich element (CRE) in the MOR 3' UTR; knockdown of PCBP1 decreases MOR mRNA half-life and protein expression. Phosphorylation of PCBP1 by PKA (via forskolin/cAMP) at serine residues promotes cytoplasmic localization and enhanced PCBP1/MOR 3'-UTR interaction. AUF1, also phosphorylated under these conditions, forms an increased interaction with PCBP1 and binds the CRE; PABP also increases its interaction with the CRE. RNA immunoprecipitation, RNA EMSA, siRNA knockdown, mRNA stability assay, phosphorylation analysis, subcellular fractionation Gene Medium 27836661
2017 PCBP1 and NCOA4 are critical for iron trafficking through ferritin in developing erythroid cells; depletion of PCBP1 impairs iron loading into ferritin, reduces heme synthesis and hemoglobin formation. Pcbp1-knockout mice develop microcytic anemia with compensatory erythropoiesis. PCBP1 delivers iron to ferritin via a direct protein-protein interaction that is developmentally regulated and enhanced by iron deprivation; excess iron inhibits this interaction in vitro. Cell depletion experiments, Pcbp1 knockout mouse model, ex vivo erythroid differentiation, ferritin iron flux assays, heme/hemoglobin measurements, in vitro protein-protein interaction assay The Journal of clinical investigation High 28375153
2017 Pcbp1 is a component of the miRNA-processing pathway in skeletal muscle; it physically interacts with Argonaute 2 (AGO2) and other miRNA pathway components, modulating the processing of muscle-enriched miRNAs (miR-1, miR-133, miR-206). Hypomorphic Pcbp1 mutant mice show muscle growth defects due to impaired myoblast proliferation/differentiation and a slow-to-fast myofibril switch. siRNA knockdown in myoblasts, co-immunoprecipitation with AGO2, hypomorphic Pcbp1 mouse model, miRNA biogenesis assays The Journal of biological chemistry Medium 28381556
2017 During erythroid differentiation, PCBP1 directly delivers iron to ferritin via protein-protein interaction; this interaction is regulated by cellular iron levels (enhanced by iron deprivation, inhibited by iron excess) and is developmentally regulated. NCOA4 activity and HERC2-mediated lysosomal degradation of NCOA4 are also regulated by cellular iron levels. In vitro protein-protein interaction assay (iron-dependent), cellular iron manipulation, developmental stage analysis Blood cells, molecules & diseases Medium 29032941
2017 TIA1 and Pcbp1 bind a UUUUCCCCCC motif between the branch point and 3' splice site of protein 4.1R exon 16 in a collaborative manner to promote exon inclusion; they associate in a complex with RBM39, which interacts with U2AF65 and SF3b155 to promote U2 snRNP recruitment to the branch point and enhance spliceosome A complex formation. RNA binding assays with mutant motifs, co-immunoprecipitation of TIA1/Pcbp1/RBM39 complex, splicing reporter assays, spliceosome assembly assays Molecular and cellular biology Medium 28193846
2017 Mutant HSPB1-P182L has a specifically increased interaction with PCBP1 compared to wild-type HSPB1, resulting in reduced translational repressive activity of PCBP1. RNA immunoprecipitation followed by RNA-seq in mouse brain identified PCBP1 mRNA targets enriched for a CTCCTCCTCCTCC consensus motif and neuronal transcripts including genes associated with hereditary neuropathies. Co-immunoprecipitation, RNA immunoprecipitation followed by RNA-seq (RIP-seq), in vitro binding Acta neuropathologica communications Medium 28077174
2017 L-homocysteine triggers a dose-dependent high-affinity interaction between hnRNP E1 and a 25-nt cis-element in the 5' UTR of hnRNP-E1 mRNA itself, leading to auto-upregulation of hnRNP E1 translation (positive feedback loop); this interaction is disrupted by ferrous iron in a dose-dependent manner. In vitro RNA-protein binding assays, in vitro translation, antisense oligonucleotide and mutant cis-element perturbations, siRNA knockdown, cell transfection with mutant hnRNP E1 The Journal of nutrition Medium 28250194
2018 PCBP1 promotes mRNA stability of Csf2 (GM-CSF) and Il2 by recognizing UC-rich elements in their 3' UTRs; intracellular iron protects PCBP1 protein from caspase-mediated proteolysis, thereby linking iron levels to T cell cytokine production. Pcbp1 deficiency in T cells inhibits GM-CSF production and abolishes capacity to induce experimental autoimmune encephalomyelitis. T cell-specific Pcbp1 knockout, Csf2 3' UTR activity assays, mRNA stability assays, caspase cleavage assays, RNA binding assays Immunity High 29958803
2018 PCBP1 specifically binds heavily oxidized RNA containing two nearby 8-oxoguanine (8-oxoG) residues (but not RNA with a single 8-oxoG); PCBP1-deficient HeLa cells (CRISPR-Cas9) show higher survival, lower caspase-3 activation, and lower PARP-1 cleavage under hydrogen peroxide treatment. KH domain mutations that disrupt RNA binding abolish this pro-apoptotic function. CRISPR-Cas9 knockout, RNA binding assays with oxidized RNA substrates, KH domain mutagenesis, caspase-3/PARP-1 cleavage assays, cell survival assays Proceedings of the National Academy of Sciences of the United States of America High 29891675
2018 PCBP1 binds p27Kip1 3' UTR via its KH1 domain, primarily stabilizing p27 mRNA and also enhancing its translation; this increases p27 protein levels, inhibits cell proliferation and cell cycle progression, and reduces tumorigenesis in vivo. Transcriptome-wide RIP screen, point mutation/luciferase assay for binding site, mRNA stability assay, ribosome profiling, cell cycle and xenograft assays Journal of experimental & clinical cancer research Medium 30086790
2019 PCBP1 coordinates ferrous iron via conserved cysteine and glutamate residues and a noncovalently bound glutathione (GSH) molecule; the Fe-GSH-bound form of PCBP1 forms a complex with cytosolic BolA2 via a bridging Fe ligand. PCBP1-Fe-GSH-BolA2 serves as an intermediate complex required for assembly of [2Fe-2S] clusters on BolA2-Glrx3, linking the ferrous iron and Fe-S distribution systems in cells. Proteomics (PCBP1 interactome), in vitro biochemical reconstitution of iron-GSH-PCBP1-BolA2 complex, site-directed mutagenesis of Fe-coordinating residues, [2Fe-2S] cluster assembly assays in vitro and in cells Nature chemical biology High 31406370
2019 PCBP1 inhibits alternative splicing of STAT3 exon 23 by binding an exonic splicing suppressor (ESS) in exon 23, thereby reducing the proportion of oncogenic STAT3α and increasing tumor-suppressive STAT3β. Overexpression/knockdown, RT-PCR splicing assay, luciferase/reporter assay for ESS, western blot for STAT3 isoforms International journal of biological sciences Medium 31223278
2020 PCBP1 facilitates binding of cGAS to cytosolic DNA; PCBP1 directly binds DNA and enhances cGAS-DNA interaction, promoting cGAS activation and innate immune signaling. PCBP1 is recruited to cGAS in a viral infection-dependent manner, and PCBP1 deficiency inhibits cGAS-mediated downstream gene transcription. Biochemical purification approach, co-immunoprecipitation (virus infection-dependent), DNA-binding assays, cGAS activation assays, PCBP1 knockdown with interferon-response readout Cellular & molecular immunology Medium 32415261
2020 TGF-β1 induces phosphorylation, poly-ubiquitination, and proteasomal degradation of PCBP1 in prostate cancer cells; loss of PCBP1 leads to increased CD44s (standard) isoform expression at the expense of CD44v, promoting EMT and cancer stem cell markers. Phosphorylation/ubiquitination assays, proteasome inhibitor experiments, RNAi knockdown, splicing assay (RT-PCR), in vitro and in vivo migration/invasion/tumor initiation assays Cellular and molecular life sciences Medium 32440711
2020 PCBP2, like PCBP1, selectively binds RNA carrying two nearby 8-oxoG residues; however, PCBP2 suppresses apoptosis under oxidative conditions while PCBP1 promotes it. PCBP2 mutations in conserved RNA-binding domains abolish 8-oxoG-RNA binding and the anti-apoptotic function. PCBP2-knockout cells show increased apoptosis, opposite to PCBP1-knockout cells. CRISPR-Cas9 KO of PCBP1 and PCBP2, RNA binding assays, KH domain mutagenesis, caspase-3/PARP1 cleavage assays, rescue with WT vs. mutant proteins The Journal of biological chemistry High 32647012
2020 Small molecule amplifiers bind directly to PCBP1 and require a PCBP1 consensus element within the CFTR open reading frame; amplifiers stabilize CFTR mRNA co-translationally and increase its association with polysomes, identifying PCBP1 as mediating CFTR mRNA stability and translational efficiency. Chemical proteomics (pulldown of amplifier-binding protein), polysome fractionation, PCBP1 consensus element mutation, mRNA stability assay Journal of cystic fibrosis Medium 32067958
2020 PCBP1 is an intracellular immune checkpoint in T cells; T cell-specific deletion of Pcbp1 favors regulatory T cell (Treg) differentiation over effector T cell (Teff) identity, upregulates inhibitory immune checkpoints (PD-1, TIGIT, VISTA) on tumor-infiltrating lymphocytes, and blunts antitumor immunity. T cell-specific Pcbp1 conditional knockout, flow cytometry for Treg/Teff markers and checkpoint molecules, tumor challenge models Science advances Medium 32523987
2021 PCBP1 deletion in mouse liver does not affect mitochondrial iron balance but impairs mitochondrial function: PCBP1 deletion reduces respiratory complexes II and IV, oxygen consumption, and ATP production, and depletes mitochondrial lipids cardiolipin and coenzyme Q; the dysfunction is caused by oxidative damage from unchaperoned (chemically reactive) iron. Liver-specific Pcbp1 knockout mouse, mitochondrial morphology analysis, respiratory complex quantification, oxygen consumption assay, mitochondrial lipid analysis, vitamin E and CoQ supplementation Free radical biology & medicine Medium 34455040
2021 PCBP1 and PCBP2 are jointly required for erythroid differentiation; combined inactivation of both loci in erythroid lineage leads to midgestational repression of erythroid/hematopoietic gene expression, loss of blood formation, and fetal demise, with overlapping and isoform-specific impacts on the erythroid transcriptome including mRNA representation and exon splicing. Lineage-specific conditional knockout of Pcbp1 and Pcbp2 in mouse embryo, RNA-seq (mRNA representation and splicing analysis), ex vivo erythroid progenitor depletion experiments Molecular and cellular biology Medium 34180713
2022 The E3 ubiquitin ligase ARIH1 interacts with hnRNP E1 (PCBP1) and promotes its ubiquitin-mediated degradation; ARIH1 silencing increases hnRNP E1 protein stability and reduces ubiquitination, while ARIH1 overexpression reduces hnRNP E1 levels. Loss of ARIH1 delays EMT induction and reduces invasion; overexpression promotes EMT and invasion in a manner dependent on hnRNP E1. Yeast two-hybrid screening, co-immunoprecipitation, ubiquitination assay, siRNA/overexpression with EMT phenotype readout, miniTurboID proximity labeling Oncogene Medium 35102251
2022 Dopamine D1 receptor signaling synergizes with ALK4/Smad3 pathway to activate PCBP1 in medium spiny neurons; PCBP1 and Smad3 interact, translocate to the nucleus, and bind to sequences in exon-4 and intron-4 of FosB pre-mRNA to promote alternative splicing and generation of ΔFosB mRNA. Ablation of either ALK4 or PCBP1 in MSNs impairs ΔFosB mRNA induction and behavioral sensitization to cocaine. Co-immunoprecipitation of PCBP1/Smad3, nuclear translocation imaging, RIP at FosB exon-4/intron-4, conditional neuronal knockout of ALK4 and PCBP1, behavioral sensitization assays The EMBO journal Medium 35730718
2022 PCBP1 acts as an adapter for the ac4C writer enzyme NAT10 in mammalian cells, tethering NAT10 to mRNA substrates for N4-acetylcytidine (ac4C) modification; knockdown of PCBP1 (or TDP43) decreases mRNA acetylation abundance and ablates cytidine-rich ac4C motifs in HEK293T cells. The NAT10/PCBP1/TDP43 complex was identified biochemically. Co-immunoprecipitation of NAT10/PCBP/TDP43 complex, siRNA knockdown, transcriptome-wide ac4C mapping, motif analysis Advanced science Medium 39556689
2022 PCBP1 regulates transcription by binding to poly(C) DNA tracts at transcription start sites; PCBP1 interacts with the G-quadruplex-specific RNA/DNA helicase DHX9 (RNA Helicase A) to modulate DHX9 accumulation and activity. PCBP1 depletion causes defects in R-loop processing and dysregulation of transcription of PCBP1 target genes. ChIP-seq (PCBP1 DNA-binding sites), co-immunoprecipitation with helicases including DHX9, R-loop assays, siRNA depletion with transcription readout The Journal of biological chemistry Medium 39342995
2023 PCBP1 binds directly to LACTB mRNA and promotes its degradation; PCBP1 knockdown increases LACTB expression, which promotes erastin-induced ferroptosis and mitochondrial dysfunction in bladder cancer cells. This binding was validated by RNA pull-down, RIP, and luciferase assays. RNA pull-down, RNA immunoprecipitation, luciferase reporter assay, PCBP1 knockdown/overexpression, ferroptosis assays, xenograft model Molecular carcinogenesis Medium 37157950
2023 PCBP1 acts as a regulator of CCL2 expression in macrophages; RNA immunoprecipitation indicated PCBP1 interacts with Ccl2 mRNA. Conditional depletion of Pcbp1 in macrophages decreases CCL2 production by colonic CX3CR1+ macrophages, reduces inflammatory macrophage accumulation, and protects mice from DSS-induced colitis. Macrophage-specific Pcbp1 conditional knockout, RNA immunoprecipitation, colitis model, cytokine measurement International immunology Medium 36702545
2023 PCBP1 binds to and enhances the stability of TPM3 mRNA in esophageal squamous cell carcinoma by directly binding the 3' UTR, as validated by RIP; PCBP1 knockdown reduces TPM3 mRNA levels and increases its degradation rate, attenuating cell migration and invasion. RIP assay, actinomycin D mRNA stability assay, siRNA knockdown, migration/invasion assay Bioengineered Low 35287546
2023 PCBP1 stabilizes DKK1 mRNA by direct binding (validated by RIP and RNA pulldown); PCBP1 knockdown decreases DKK1 mRNA stability and protein levels, relieving inhibition of the Wnt/β-catenin pathway and promoting lung adenocarcinoma progression. RNA-seq of PCBP1 knockdown cells, RIP, RNA pulldown, mRNA stability assay, western blot, in vivo xenograft Journal of translational medicine Low 35907982
2023 PCBP1 KH3 domain mediates its interaction with the HTLV-1 Tax oncoprotein in the cytoplasm; PCBP1 facilitates assembly of the Tax/IKK complex, thereby potentiating Tax-mediated IKK-NF-κB signaling. PCBP1 knockdown attenuates Tax-dependent NF-κB activation and reduces proliferation of HTLV-1-transformed cells. Co-immunoprecipitation, colocalization assay, KH3 domain deletion/mutation analysis, knockdown with NF-κB reporter, IKK activity assay, cell proliferation assay Frontiers in immunology Medium 38690287
2024 PCBP1 interacts with BCAT2 (branched-chain amino acid transaminase 2) at Leucine 239 of BCAT2; this interaction co-regulates the PI3K/AKT signaling pathway to inhibit autophagy, autophagy-related apoptosis, and ferroptosis in prostate cancer cells. Co-immunoprecipitation, mass spectrometry, Leu239 point mutation, RNA sequencing, functional autophagy/apoptosis/ferroptosis assays Cell death & disease Medium 40274762
2025 PCBP1 undergoes O-GlcNAcylation at Threonine 99 in the cytoplasm (mediated by OGT in the presence of circPRELID2); this modification disrupts PCBP1 binding to the 3' UTR of ZEB2 mRNA, reversing ZEB2 translational silencing and promoting EMT and metastasis of gastric cancer cells. RIP for PCBP1/ZEB2 3'-UTR interaction, site-specific O-GlcNAc mutagenesis (T99), circPRELID2 knockdown/overexpression, OGT assay, polysome profiling, in vivo metastasis models Advanced science Medium 41117067

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end. Cell 441 9160751
2010 TGF-beta-mediated phosphorylation of hnRNP E1 induces EMT via transcript-selective translational induction of Dab2 and ILEI. Nature cell biology 265 20154680
2010 Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles. RNA (New York, N.Y.) 223 20584894
2011 Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2. Cell metabolism 176 22055506
2010 PCBP1 suppresses the translation of metastasis-associated PRL-3 phosphatase. Cancer cell 154 20609352
2017 PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis. The Journal of clinical investigation 144 28375153
2018 Iron Drives T Helper Cell Pathogenicity by Promoting RNA-Binding Protein PCBP1-Mediated Proinflammatory Cytokine Production. Immunity 129 29958803
2014 Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase. Proceedings of the National Academy of Sciences of the United States of America 113 24843120
2019 A PCBP1-BolA2 chaperone complex delivers iron for cytosolic [2Fe-2S] cluster assembly. Nature chemical biology 111 31406370
2007 Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1. Proceedings of the National Academy of Sciences of the United States of America 98 17389360
2020 Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1. Journal of experimental & clinical cancer research : CR 78 32552766
2010 PCBP-1 regulates alternative splicing of the CD44 gene and inhibits invasion in human hepatoma cell line HepG2 cells. Molecular cancer 77 20361869
2016 Direct Regulation of Alternative Splicing by SMAD3 through PCBP1 Is Essential to the Tumor-Promoting Role of TGF-β. Molecular cell 71 27746021
2006 Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs. Molecular biology of the cell 71 16775011
2011 Poly(C)-binding protein 1 (PCBP1) mediates housekeeping degradation of mitochondrial antiviral signaling (MAVS). Cell research 68 22105485
2015 The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development. Molecular and cellular biology 61 26527618
2002 Suppression of 15-lipoxygenase synthesis by hnRNP E1 is dependent on repetitive nature of LOX mRNA 3'-UTR control element DICE. Journal of molecular biology 58 11827469
2017 Ferritin iron regulators, PCBP1 and NCOA4, respond to cellular iron status in developing red cells. Blood cells, molecules & diseases 57 29032941
2018 Specific binding of PCBP1 to heavily oxidized RNA to induce cell death. Proceedings of the National Academy of Sciences of the United States of America 56 29891675
2018 PCBP1 depletion promotes tumorigenesis through attenuation of p27Kip1 mRNA stability and translation. Journal of experimental & clinical cancer research : CR 54 30086790
2013 Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs. Molecular and cellular biology 53 23478261
2020 PCBP1 modulates the innate immune response by facilitating the binding of cGAS to DNA. Cellular & molecular immunology 51 32415261
2020 TGF-β1 promotes epithelial-to-mesenchymal transition and stemness of prostate cancer cells by inducing PCBP1 degradation and alternative splicing of CD44. Cellular and molecular life sciences : CMLS 46 32440711
2005 Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C. Biochemical and biophysical research communications 45 16248985
2021 LncRNA PCBP1-AS1-mediated AR/AR-V7 deubiquitination enhances prostate cancer enzalutamide resistance. Cell death & disease 41 34545063
2020 RNA binding protein PCBP1 is an intracellular immune checkpoint for shaping T cell responses in cancer immunity. Science advances 40 32523987
2020 Amplifiers co-translationally enhance CFTR biosynthesis via PCBP1-mediated regulation of CFTR mRNA. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 39 32067958
2019 PCBP1 inhibits the expression of oncogenic STAT3 isoform by targeting alternative splicing of STAT3 exon 23. International journal of biological sciences 38 31223278
2021 PCBP1 regulates the transcription and alternative splicing of metastasis‑related genes and pathways in hepatocellular carcinoma. Scientific reports 37 34857818
2015 Expression of poly(C)-binding protein 1 (PCBP1) in NSCLC as a negative regulator of EMT and its clinical value. International journal of clinical and experimental pathology 35 26261610
2021 Mitochondrial dysfunction in mouse livers depleted of iron chaperone PCBP1. Free radical biology & medicine 34 34455040
2020 PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions. The Journal of biological chemistry 34 32647012
2012 THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. FEBS letters 32 22673507
2022 The ubiquitin E3 ligase ARIH1 regulates hnRNP E1 protein stability, EMT and breast cancer progression. Oncogene 31 35102251
2015 Translational regulation of inhibin βA by TGFβ via the RNA-binding protein hnRNP E1 enhances the invasiveness of epithelial-to-mesenchymal transitioned cells. Oncogene 31 26096938
2017 Expression of both poly r(C) binding protein 1 (PCBP1) and miRNA-3978 is suppressed in peritoneal gastric cancer metastasis. Scientific reports 30 29138420
2018 The RNA-Binding Protein PCBP1 Functions as a Tumor Suppressor in Prostate Cancer by Inhibiting Mitogen Activated Protein Kinase 1. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 28 30078000
2012 PCBP1 is required for maintenance of the transcriptionally silent state in fully grown mouse oocytes. Cell cycle (Georgetown, Tex.) 27 22801551
2018 TGF-beta signaling in cancer: post-transcriptional regulation of EMT via hnRNP E1. Cytokine 26 29396052
2017 Mutant HSPB1 causes loss of translational repression by binding to PCBP1, an RNA binding protein with a possible role in neurodegenerative disease. Acta neuropathologica communications 25 28077174
2015 The PCBP1 gene encoding poly(rC) binding protein I is recurrently mutated in Burkitt lymphoma. Genes, chromosomes & cancer 25 26173642
2008 Identification of transcripts and translatants targeted by overexpressed PCBP1. Biochimica et biophysica acta 25 18656558
2023 PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation. Molecular carcinogenesis 24 37157950
2024 A small protein encoded by PCBP1-AS1 is identified as a key regulator of influenza virus replication via enhancing autophagy. PLoS pathogens 23 39137200
2011 Incrimination of heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1) as a candidate sensor of physiological folate deficiency. The Journal of biological chemistry 23 21930702
2025 Acevaltrate as a novel ferroptosis inducer with dual targets of PCBP1/2 and GPX4 in colorectal cancer. Signal transduction and targeted therapy 22 40619432
2017 Protein 4.1R Exon 16 3' Splice Site Activation Requires Coordination among TIA1, Pcbp1, and RBM39 during Terminal Erythropoiesis. Molecular and cellular biology 22 28193846
2017 Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts. The Journal of biological chemistry 22 28381556
2016 PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Molecular cancer research : MCR 21 27102006
2016 Phosphorylation of poly(rC) binding protein 1 (PCBP1) contributes to stabilization of mu opioid receptor (MOR) mRNA via interaction with AU-rich element RNA-binding protein 1 (AUF1) and poly A binding protein (PABP). Gene 21 27836661
2014 SchA-p85-FAK complex dictates isoform-specific activation of Akt2 and subsequent PCBP1-mediated post-transcriptional regulation of TGFβ-mediated epithelial to mesenchymal transition in human lung cancer cell line A549. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 21 24819169
2020 lncRNA PCBP1-AS1 Aggravates the Progression of Hepatocellular Carcinoma via Regulating PCBP1/PRL-3/AKT Pathway. Cancer management and research 20 32753957
2017 Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2. FEBS letters 20 28027391
2010 Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1. Biochimica et biophysica acta 20 20624489
2022 The RNA-binding protein PCBP1 represses lung adenocarcinoma progression by stabilizing DKK1 mRNA and subsequently downregulating β-catenin. Journal of translational medicine 19 35907982
2016 Identification and characterization of an hnRNP E1 translational silencing motif. Nucleic acids research 19 27067543
2025 TBBPA induced hepatocyte ferroptosis by PCBP1-mediated ferritinophagy. Journal of hazardous materials 16 40359755
2022 Convergent dopamine and ALK4 signaling to PCBP1 controls FosB alternative splicing and cocaine behavioral sensitization. The EMBO journal 16 35730718
2021 Using lncRNA Sequencing to Reveal a Putative lncRNA-mRNA Correlation Network and the Potential Role of PCBP1-AS1 in the Pathogenesis of Cervical Cancer. Frontiers in oncology 16 33833992
2021 RNA-Binding Proteins PCBP1 and PCBP2 Are Critical Determinants of Murine Erythropoiesis. Molecular and cellular biology 15 34180713
2014 PCBP1 is an important mediator of TGF-β-induced epithelial to mesenchymal transition in gall bladder cancer cell line GBC-SD. Molecular biology reports 15 24889597
2023 LncRNA PCBP1-AS1 induces osteoporosis by sponging miR-126-5p/PAK2 axis. Bone & joint research 14 37306572
2021 LncRNA PCBP1-AS1 correlated with the functional states of cancer cells and inhibited lung adenocarcinoma metastasis by suppressing the EMT progression. Carcinogenesis 14 34107009
2020 SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs via Up-Regulating GNAI2 and PCBP1. Frontiers in pharmacology 13 32536864
2019 hnRNP E1 at the crossroads of translational regulation of epithelial-mesenchymal transition. Journal of cancer metastasis and treatment 13 31681852
2017 Evidence Favoring a Positive Feedback Loop for Physiologic Auto Upregulation of hnRNP-E1 during Prolonged Folate Deficiency in Human Placental Cells. The Journal of nutrition 13 28250194
2016 Post-Transcriptional Regulation of the Human Mu-Opioid Receptor (MOR) by Morphine-Induced RNA Binding Proteins hnRNP K and PCBP1. Journal of cellular physiology 13 27292014
2025 BCAT2 binding to PCBP1 regulates the PI3K/AKT signaling pathway to inhibit autophagy-related apoptosis and ferroptosis in prostate cancer. Cell death & disease 12 40274762
2022 PCBP-1 Regulates the Transcription and Alternative Splicing of Inflammation and Ubiquitination-Related Genes in PC12 Cell. Frontiers in aging neuroscience 12 35795237
2024 LncRNA GAS6-AS1 contributes to 5-fluorouracil resistance in colorectal cancer by facilitating the binding of PCBP1 with MCM3. Cancer letters 11 38521199
2021 Host Restriction Factor A3G Inhibits the Replication of Enterovirus D68 by Competitively Binding the 5' Untranslated Region with PCBP1. Journal of virology 11 34730395
2014 Specific enrichment of the RNA-binding proteins PCBP1 and PCBP2 in chief cells of the murine gastric mucosa. Gene expression patterns : GEP 11 24480778
2023 Inhibition of apoptosis-regulatory protein Siva-1 reverses multidrug resistance in gastric cancer by targeting PCBP1. Oncology research 10 37303491
2023 Discovery of tetrahydroisoquinolineindole derivatives as first dual PRMT5 inhibitors/hnRNP E1 upregulators: Design, synthesis and biological evaluation. European journal of medicinal chemistry 10 37429083
2022 PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma. Bioengineered 10 35287546
2020 Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. Biochimica et biophysica acta. Molecular basis of disease 10 32480040
2024 PCBP1/2 and TDP43 Function as NAT10 Adaptors to Mediate mRNA ac4C Formation in Mammalian Cells. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9 39556689
2022 PCBP1 is associated with rheumatoid arthritis by affecting RNA products of genes involved in immune response in Th1 cells. Scientific reports 9 35589811
2019 Effects of antisense lncRNA PCBP1-AS1 on biological behaviors of vulvar squamous carcinoma cells by regulating TRAF5 and NF-κB expression. Translational cancer research 9 35116901
2024 PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation. Frontiers in immunology 8 38690287
2024 The RNA-binding protein PCBP1 modulates transcription by recruiting the G-quadruplex-specific helicase DHX9. The Journal of biological chemistry 8 39342995
2023 PCBP1 acts as a regulator of CCL2 expression in macrophages to induce recruitment of monocyte-derived macrophages into the inflamed colon. International immunology 8 36702545
2025 ECM1 protects against liver steatosis through PCBP1-mediated iron homeostasis. Hepatology (Baltimore, Md.) 7 40372944
2023 PCBP1 regulates LIFR through FAM3C to maintain breast cancer stem cell self-renewal and invasiveness. Cancer biology & therapy 7 37927213
2022 Generation of PCBP1-deficient pigs using CRISPR/Cas9-mediated gene editing. iScience 7 36274935
2021 Identification of PCBP1 as a Novel Modulator of Mammalian Circadian Clock. Frontiers in genetics 7 33841513
2025 PCBP1 modulates cellular iron homeostasis via targeting HIF-1α/HO-1 pathway and alleviates high-glucose-induced ferroptosis in HRMECs. Experimental eye research 6 40789503
2024 Investigating the neuroprotective potential of rAAV2-PCBP1-EGFP gene therapy against a 6-OHDA-induced model of Parkinson's disease. Brain and behavior 6 38376022
2023 Conserved role for PCBP1 in altered RNA splicing in the hippocampus after chronic alcohol exposure. Molecular psychiatry 6 37537282
2017 Phorbol-12-myristate-13-acetate-mediated stabilization of leukemia inhibitory factor (lif) mRNA: involvement of Nucleolin and PCBP1. The Biochemical journal 6 28512205
2009 Identification of novel partner proteins of PCBP1. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 6 19727228
2025 Hypoxia-Induced circPRELID2 Promotes Gastric Cancer Metastasis by Facilitating ZEB2 Translation via PCBP1 O-GlcNAcylation. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 5 41117067
2024 LncRNA PCBP1-AS1 suppresses cell growth in oral squamous cell carcinoma by targeting miR-34c-5p/ZFP36 axis. Cellular and molecular biology (Noisy-le-Grand, France) 5 39380272
2023 Association of LncRNA PCBP1-AS1 with cancer occurrence and development: A review. Medicine 5 37904442
2025 LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1. Scientific reports 4 39757305
2025 RNA splicing of the STAT3 by PCBP1 promotes vulnerable plaque formation via macrophage-like phenotype modulation of vascular smooth muscle cell. Atherosclerosis 4 39938358
2024 Decoding poly (RC)-binding protein 1 (PCBP1), the underrated guard at the foothill of ferroptosis. Pathology, research and practice 4 39700662
2022 PCBP1-mediated regulation of WNT signaling is critical for breast tumorigenesis. Cell biology and toxicology 4 35639300
2016 Identification of gamma-synuclein as a new PCBP1-interacting protein. Neurological research 4 26344801
2025 RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation. Molecular metabolism 3 40451383

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