Affinage

RUVBL1

RuvB-like 1 · UniProt Q9Y265

Length
456 aa
Mass
50.2 kDa
Annotated
2026-04-28
100 papers in source corpus 56 papers cited in narrative 56 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RUVBL1 is a conserved AAA+ ATPase that forms heterohexameric and dodecameric complexes with RUVBL2, serving as a central scaffolding and chaperone-like engine within multiple chromatin-remodeling, transcriptional, and macromolecular assembly machines. Structurally, RUVBL1 contains Walker A/B ATPase domains (DI/DIII) and a unique domain II that binds single-stranded DNA/RNA and undergoes conformational transitions regulating nucleotide exchange and substrate access; its ATPase activity, modulated by post-translational modifications including PRMT5-mediated R205 methylation, DTL-mediated ubiquitination, and PLK1 phosphorylation, is required for TIP60 histone acetyltransferase activity, H2A.Z exchange, γTuRC biogenesis, R2TP/PAQosome-dependent PIKK and snoRNP maturation, Fanconi anemia core complex stability, ciliary protein pre-assembly, and NMD initiation (PMID:17060327, PMID:28238654, PMID:33355144, PMID:19696079, PMID:25428364, PMID:29959317, PMID:33205750, PMID:38609375). Through its incorporation into TIP60/NuA4, INO80, and β-catenin–TCF complexes, RUVBL1 controls histone H4 acetylation states at DNA damage sites and gene promoters, thereby directing DNA repair pathway choice between homologous recombination and NHEJ, activating Wnt/β-catenin and MYC transcriptional programs, and sustaining cancer cell proliferation (PMID:18285460, PMID:14695187, PMID:9843967, PMID:38821858). Conditional knockout of Ruvbl1 in mice causes embryonic lethality, aplastic anemia in the hematopoietic compartment, and cilia-deficient renal failure and hydrocephalus, underscoring its essential roles across tissues (PMID:25428364, PMID:29959317).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1998 High

    Establishing RUVBL1 as a nuclear ATPase essential for viability and integrated into β-catenin transcriptional signaling resolved the gene's basic identity: it was not merely a TBP-associated factor but an essential component of Wnt-driven nuclear transactivation.

    Evidence Yeast two-hybrid, co-IP with β-catenin/TBP/LEF-1, yeast null-mutant lethality, detection in RNAPII holoenzyme

    PMID:9196036 PMID:9774387 PMID:9843967

    Open questions at the time
    • Enzymatic activity not yet demonstrated
    • Stoichiometry and direct subunit composition of nuclear complexes unknown
  2. 1999 High

    Demonstrating that RUVBL1 possesses DNA-stimulated ATPase and 3ʹ→5ʹ helicase activity, and that RUVBL2 has opposite-polarity helicase activity within a shared ~700 kDa complex, established the biochemical foundation for understanding RUVBL1/2 as a cooperative enzymatic machine.

    Evidence In vitro ATPase and DNA helicase assays with purified recombinant RUVBL1 and RUVBL2

    PMID:10336418 PMID:10428817

    Open questions at the time
    • Helicase activity later disputed for the isolated complex
    • Physiological substrates unidentified
  3. 2003 High

    Linking RUVBL1 ATPase activity to β-catenin-mediated transformation and showing RUVBL1 occupancy at Wnt target promoters together with TIP60 and TRRAP established RUVBL1 as a chromatin-remodeling effector of oncogenic Wnt signaling, not just a transcriptional co-activator.

    Evidence ATPase-dead D302N mutant, ChIP at ITF-2 promoter, co-IP with TIP60/TRRAP/BAF53, reporter and transformation assays

    PMID:14695187

    Open questions at the time
    • Mechanism by which ATPase activity controls histone acetylation not resolved
    • Role of centrosomal/tubulin association (also reported in 2003) unclear relative to nuclear functions
  4. 2006 High

    The 2.2 Å crystal structure of the RUVBL1 hexamer and the EM structure of the RUVBL1/2 dodecamer resolved the domain architecture—revealing domain II as a unique DNA/RNA-binding insertion absent in bacterial RuvB—and showed that the isolated complex has synergistic but low ATPase activity and no detectable helicase activity, resetting the field's understanding of intrinsic versus cofactor-stimulated activity.

    Evidence X-ray crystallography (2.2 Å), electron microscopy 3D reconstruction (20 Å), ATPase and helicase assays

    PMID:17060327 PMID:17157868

    Open questions at the time
    • Cofactors that stimulate ATPase/helicase in vivo not yet identified
    • Role of dodecamer versus hexamer unknown
  5. 2008 High

    Demonstrating that RUVBL1 depletion abolishes TIP60 HAT activity and impairs RAD51 recruitment after DNA damage established a direct mechanistic link between RUVBL1, chromatin acetylation, and homologous recombination repair.

    Evidence siRNA knockdown, HAT activity assay, γH2AX and RAD51 foci quantification, HDAC inhibitor rescue

    PMID:18285460 PMID:18834951

    Open questions at the time
    • Whether RUVBL1 is a structural or catalytic component of TIP60 complex unresolved
    • No reconstitution of TIP60 activity with purified RUVBL1
  6. 2010 High

    The discovery that RUVBL1/2 associate with all six PIKK family members, control PIKK mRNA levels, and promote SMG-1-dependent NMD initiation expanded RUVBL1's role from a chromatin factor to a general chaperone/assembly factor for PIKK-containing signaling complexes.

    Evidence Co-IP with each PIKK, siRNA knockdown effects on PIKK protein levels, NMD reporter assays, RNA-IP

    PMID:20371770

    Open questions at the time
    • Mechanism of PIKK mRNA stabilization not defined
    • Whether RUVBL1/2 act co-translationally or post-translationally on PIKKs unclear
  7. 2012 High

    Cryo-EM visualization of two coexisting dodecamer conformations (compact/stretched) driven by domain II movements, together with the discovery that the TTT-RUVBL1/2 complex senses cellular energy to control mTORC1 assembly and lysosomal localization, connected the structural dynamics of the RUVBL ring to a physiological metabolic sensing mechanism.

    Evidence Cryo-EM image classification, co-IP with TTT/mTORC1, metabolic perturbation, lysosomal fractionation

    PMID:23002137 PMID:23142078

    Open questions at the time
    • Structural basis of TTT-RUVBL interaction not yet resolved
    • Whether conformational switching directly gates client loading unknown
  8. 2015 High

    Identification of PLK1-mediated phosphorylation of RUVBL1 and the severe mitotic defects upon RUVBL1 depletion established RUVBL1 as a mitotically regulated factor required for chromosome segregation, complementing its interphase chromatin functions.

    Evidence In vitro kinase assay, co-IP with PLK1, siRNA knockdown causing chromosome misalignment, ATPase-dead mutant growth arrest

    PMID:26201077

    Open questions at the time
    • PLK1 phosphorylation sites on RUVBL1 not mapped
    • Downstream mitotic substrates of phospho-RUVBL1 unknown
  9. 2017 High

    PRMT5-mediated arginine methylation of RUVBL1 at R205 was shown to be a prerequisite for TIP60 HAT activity and 53BP1 displacement at DSBs, establishing a post-translational modification code that switches DNA repair from NHEJ toward homologous recombination. Simultaneously, cryo-EM and biochemistry showed that the INO80 insertion domain stimulates RUVBL ATPase 16-fold while driving dodecamer-to-hexamer transitions, revealing the structural basis for cofactor-dependent activation.

    Evidence In vitro methylation, R205A mutagenesis, HAT assay, 53BP1/RAD51 foci ChIP; cryo-EM of Rvb1/2-Ino80INS, ATPase stimulation quantification

    PMID:28238654 PMID:28591576

    Open questions at the time
    • Whether R205 methylation status changes during cell cycle unknown
    • Structural basis of methylation-dependent TIP60 activation unresolved
  10. 2018 High

    Conditional mouse knockouts established that RUVBL1 is essential for motile cilia biogenesis (hydrocephalus upon loss), renal epithelial integrity, and left-right symmetry determination, providing the first in vivo evidence that RUVBL1 functions as a cytoplasmic assembly factor for ciliary protein complexes.

    Evidence Conditional Ruvbl1 KO in renal tubular and ciliated cells, zebrafish rescue, co-IP with DNAAF1/IFT88

    PMID:29228333 PMID:29959317

    Open questions at the time
    • Identity of specific ciliary protein clients assembled by RUVBL1 not catalogued
    • Whether R2TP complex mediates ciliary assembly function not tested
  11. 2020 High

    Cryo-EM of the DHX34–RUVBL1/2 complex showed that DHX34 locks all RUVBL2 subunits in a nucleotide-free conformation to down-regulate ATPase activity, directly coupling RUVBL catalytic cycling to NMD initiation; separately, reconstitution showed RUVBL1/2 assembles functional γTuRC from minimal components, broadening the chaperone paradigm to microtubule nucleation complexes.

    Evidence Cryo-EM of DHX34-RUVBL complex, ATPase assays with mutants; coexpression reconstitution of γTuRC, cryo-EM (~4 Å), microtubule nucleation assay

    PMID:33205750 PMID:33355144

    Open questions at the time
    • How RUVBL1/2 release assembled γTuRC not mechanistically defined
    • Whether DHX34-mediated ATPase inhibition is reversible in cells unknown
  12. 2023 Medium

    Identification of GART-mediated lysine K7 methylation stabilizing RUVBL1 to activate Wnt/β-catenin signaling, and demonstration that RUVBL1 controls MYC chromatin occupancy and EEF1A1 expression in Ewing sarcoma, revealed new layers of post-translational and transcriptional regulation centering RUVBL1 in oncogenic networks.

    Evidence In vitro methylation assay with GART E948/K7 mutagenesis, β-catenin reporter; CRISPR gene body scan, MYC ChIP-seq, ribosome profiling

    PMID:37075745 PMID:37439412

    Open questions at the time
    • GART methyltransferase activity on RUVBL1 not confirmed by independent lab
    • Structural basis of K7 methylation-dependent stabilization unknown
  13. 2024 High

    DTL-mediated ubiquitination of RUVBL1 was shown to redirect it from TIP60 toward β-catenin, switching DNA repair from HR to NHEJ; separately, auxin-degron-mediated RUVBL1 degradation caused complete pancreatic tumor regression with immune cell infiltration, establishing RUVBL1 as a druggable MYC effector with immunomodulatory consequences.

    Evidence Ubiquitination assay, HAT/repair pathway assays, siRNA; auxin-degron in vivo, RNA-seq, shRNA screens, xenografts

    PMID:38609375 PMID:38821858

    Open questions at the time
    • DTL-RUVBL1 ubiquitination sites not mapped
    • Mechanism linking RUVBL1 loss to immune infiltration not defined
    • Whether ubiquitination is cell-cycle regulated unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: how RUVBL1 selects among its diverse client complexes (TIP60, R2TP, γTuRC, FA core, INO80), whether domain II conformational dynamics serve as a universal gating mechanism for client loading, the structural basis of post-translational modification crosstalk (methylation, ubiquitination, phosphorylation) on RUVBL1 function, and whether RUVBL1's chaperone versus enzymatic activities are separable in vivo.
  • No unified structural model of how RUVBL1 discriminates clients
  • No separation-of-function mutant distinguishing chaperone from ATPase roles in vivo
  • In vivo stoichiometry of hexamer versus dodecamer pools and their functional assignment unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140657 ATP-dependent activity 9 GO:0098772 molecular function regulator activity 4 GO:0003677 DNA binding 3 GO:0044183 protein folding chaperone 3 GO:0003723 RNA binding 1
Localization
GO:0005634 nucleus 6 GO:0005694 chromosome 3 GO:0005829 cytosol 3 GO:0005815 microtubule organizing center 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-4839726 Chromatin organization 7 R-HSA-162582 Signal Transduction 6 R-HSA-73894 DNA Repair 6 R-HSA-74160 Gene expression (Transcription) 6 R-HSA-8953854 Metabolism of RNA 4 R-HSA-1640170 Cell Cycle 3 R-HSA-1852241 Organelle biogenesis and maintenance 3
Partners
CTNNB1DHX34KAT5PIH1D1PRMT5RPAP3RUVBL2TRRAP
Complex memberships
INO80R2TP/PAQosomeRUVBL1-RUVBL2 heterohexamer/dodecamerTIP60/NuA4

Evidence

Reading pass · 56 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 RUVBL1 (TIP49/TIP49a) was identified as a TBP-interacting protein that co-immunoprecipitates with TBP in a nuclear complex, and its sequence contains Walker A and B motifs homologous to bacterial RuvB, suggesting ATPase/helicase activity. Affinity purification with histidine-tagged TBP, immunoprecipitation, sequence analysis Biochemical and biophysical research communications Medium 9196036
1998 RUVBL1 (Pontin52) was identified as a nuclear protein that binds beta-catenin at Armadillo repeats 2-5 and also binds the TATA-box binding protein (TBP), forming an in vivo multiprotein complex with beta-catenin and LEF-1/TCF, implicating RUVBL1 in beta-catenin-mediated nuclear transactivation. Yeast two-hybrid, co-immunoprecipitation, GST pulldown Proceedings of the National Academy of Sciences of the United States of America High 9843967
1998 RUVBL1 is an essential gene; yeast null mutants of the RUVBL1 homolog (scRUVBL1) are nonviable. RUVBL1 was co-immunoprecipitated with at least three other cellular proteins and was detected in the RNA polymerase II holoenzyme complex. Yeast two-hybrid (identification with RPA3 as bait), co-immunoprecipitation, yeast null mutant viability assay The Journal of biological chemistry High 9774387
1999 RUVBL1 (TIP49a/TIP49) possesses ATPase activity stimulated by single-stranded DNA and ATP-dependent DNA helicase activity with 3'-to-5' directionality, as demonstrated with recombinant purified protein. In vitro ATPase assay, DNA helicase assay, UV cross-linking assay with purified recombinant protein The Journal of biological chemistry High 10336418
1999 RUVBL2 (TIP49b) forms a physical complex with RUVBL1 (TIP49a) in a ~700 kDa cellular complex, has ATPase activity stimulated by single-stranded DNA, and ATP-dependent DNA helicase activity with 5'-to-3' directionality (opposite polarity to RUVBL1). Both genes are essential in S. cerevisiae. Co-immunoprecipitation, in vitro ATPase and helicase assays, yeast genetic complementation The Journal of biological chemistry High 10428817
2000 RUVBL1 (Pontin52) and its homolog RUVBL2 (Reptin52) function as antagonistic regulators of beta-catenin/TCF transcriptional activity: RUVBL1 activates while RUVBL2 represses Wnt target gene transcription, and this antagonism is evolutionarily conserved in Drosophila (dpontin/dreptin). Reporter gene assays, co-immunoprecipitation, Drosophila genetic experiments (Wingless signaling) The EMBO journal High 11080158
2001 RUVBL1 (TIP49a) functions as a plasminogen-binding protein on the U937 cell surface, with a C-terminal lysine mediating plasminogen binding (Kd ~0.57 μM) and enhancing plasminogen activation ~8-fold. Protein purification, carboxypeptidase B treatment, ligand blotting with 125I-plasminogen, plasminogen activation assay The Journal of biological chemistry Medium 11027681
2002 RUVBL1 (TIP49) binds the E2F1 transactivation domain and modulates both E2F1-mediated transformation and apoptosis, while also being required for c-Myc-mediated apoptosis (unlike TRRAP which does not affect apoptosis). Co-immunoprecipitation, dominant-negative overexpression, apoptosis assays Oncogene Medium 12185582
2003 RUVBL1 (TIP49) ATPase activity is required for beta-catenin-mediated neoplastic transformation and TCF-dependent gene activation. RUVBL1 was found in complexes with chromatin-remodeling and histone-modifying factors including TIP60, TRRAP, and BAF53, and chromatin immunoprecipitation showed RUVBL1, TIP60, and TRRAP occupying the ITF-2 promoter; RUVBL1 ATPase-dead mutant decreased histone acetylation at TCF-binding sites. ATPase-deficient mutant (D302N), siRNA knockdown, reporter gene assay, co-immunoprecipitation, chromatin immunoprecipitation Cancer research High 14695187
2003 RUVBL1 associates with alpha- and gamma-tubulin, localizes to the centrosome and mitotic spindle during mitosis in a stage-dependent manner (metaphase: centrosome and astral microtubules; anaphase B: polar tubule interdigitation zone), and promotes in vitro tubulin assembly. Protein affinity chromatography, co-immunoprecipitation, confocal immunofluorescence microscopy, in vitro tubulin assembly assay Cell motility and the cytoskeleton Medium 14506706
2003 RUVBL1 (Pontin52/TIP49a) promotes COX-2 expression in colon cancer cells and co-localizes with LEF-1 at the COX-2 promoter, linking RUVBL1 to Wnt-pathway-dependent gene regulation. Overexpression, siRNA knockdown, reporter assay Molecular cancer Low 14675489
2005 Drosophila Pont (Pontin/RUVBL1 ortholog) physically binds dMyc and shows strong dominant genetic interaction with dmyc in controlling tissue growth, cell competition, and repression of specific target genes (e.g., mfas). Co-immunoprecipitation, genetic interaction analysis, mitotic clone analysis in Drosophila Proceedings of the National Academy of Sciences of the United States of America High 16087886
2006 Crystal structure of human RuvBL1 hexamer at 2.2 Å resolution revealed three domains: domains I and III involved in ATP binding/hydrolysis, and domain II (unique among AAA+ proteins, absent from bacterial RuvB) functioning as a novel DNA/RNA-binding domain. RuvBL1 binds single-stranded DNA/RNA and double-stranded DNA, but only marginal ATPase activity was detected with purified protein alone, suggesting requirement for cofactors. X-ray crystallography (2.2 Å), DNA binding assays, ATPase assay The Journal of biological chemistry High 17060327
2006 The human TIP48/TIP49 (RUVBL2/RUVBL1) complex forms a dodecameric double-ring structure (as shown by electron microscopy 3D reconstruction at 20 Å), with synergistic ATPase activity requiring both subunits; no DNA helicase or branch migration activity was detected for the isolated complex in vitro. Electron microscopy 3D reconstruction, ATPase assay, DNA helicase assay, catalytic mutant analysis Journal of molecular biology High 17157868
2008 Human RUVBL1 (Rvb1) is required for the histone acetyltransferase (HAT) activity of the Tip60/NuA4 complex. Depletion of RUVBL1 increases persistence of phospho-H2AX after DNA damage without increasing DNA damage itself. The mechanism involves RUVBL1 maintaining Tip60 HAT activity, which acetylates histone H4, enabling subsequent dephosphorylation of phospho-H2AX. siRNA knockdown, HAT activity assay, γH2AX immunofluorescence, histone H4 acetylation assay Molecular and cellular biology High 18285460
2008 RUVBL1 (TIP49) depletion reduces RAD51 recruitment to chromatin and nuclear foci formation (~50% reduction) after double-strand breaks and interstrand crosslinks, without affecting H2AX phosphorylation or checkpoint signaling; this reduction is rescued by HDAC inhibitor treatment, linking RUVBL1-mediated chromatin modification to homologous recombination. siRNA knockdown, immunofluorescence for RAD51 foci, chromatin fractionation The international journal of biochemistry & cell biology Medium 18834951
2008 Yeast Rvb1 and Rvb2 form a heterohexameric ring (not double-hexamer) with enhanced ATPase activity relative to individual proteins, further stimulated by dsDNA with 5' or 3' overhangs, and exhibit 5'-to-3' DNA helicase activity. Electron microscopy, in vitro ATPase assay, DNA helicase assay, analytical ultracentrifugation Journal of molecular biology High 18234224
2009 TIP48 (RUVBL2) and TIP49 (RUVBL1) play a major role in catalyzing H2A.Z exchange into nucleosomes via their ATPase activities, acting cooperatively with TIP60-mediated acetylation of nucleosomal H2A, which specifically facilitates small complex-mediated H2A.Z exchange. Biochemical purification of H2A.Z-interacting complexes, in vitro H2A.Z exchange assay, ATPase activity assay Nucleic acids research High 19696079
2010 RUVBL1 and RUVBL2 associate with each PIKK family member (including SMG-1, ATM, ATR, mTOR, DNA-PKcs, TRRAP), control PIKK mRNA abundance upon knockdown, and promote formation of mRNA surveillance complexes during nonsense-mediated mRNA decay (NMD) by associating with SMG-1 and mRNPs in the cytoplasm. Co-immunoprecipitation, siRNA knockdown, NMD reporter assays, RNA immunoprecipitation Science signaling High 20371770
2011 RUVBL1 (TIP49) represses p53 transcription by binding the p53 promoter, interfering with RNF20/hBRE1-mediated histone H2B monoubiquitination and promoting PAF1-mediated histone H3K9 trimethylation, thereby blocking p53-mediated apoptosis. EHF directly activates RUVBL1 transcription. ChIP, reporter gene assay, co-immunoprecipitation, siRNA knockdown, apoptosis assay EMBO reports Medium 21617703
2011 Hsp90 forms complexes with RUVBL1/2 and the Tel2 complex, and Hsp90 inhibition reduces levels of all PIKK family members and suppresses PIKK-mediated signaling, suggesting Hsp90, RUVBL1/2, and Tel2 form a joint regulatory complex for PIKK stability. Co-immunoprecipitation, Hsp90 inhibitor treatment, Western blot for PIKK levels Cancer science Medium 21951644
2011 Crystal structure of RuvBL1-RuvBL2 complex with truncated domain II revealed a dodecamer of two heterohexameric rings with alternating RuvBL1 and RuvBL2 monomers bound to ADP/ATP, interacting via retained domain II. Truncation of domain II increased ATP consumption substantially. Domain II auto-inhibits DNA unwinding activity of the human RuvBL proteins. X-ray crystallography, small-angle X-ray scattering, ATPase assay, DNA helicase assay Journal of structural biology High 21933716
2012 Cryo-EM structures of human double-ring RuvBL1-RuvBL2 complexes revealed two coexisting conformations (compact and stretched), driven by movements in DII domains. These conformational transitions expose/conceal DNA-binding regions, providing a mechanism linking DII dynamics to regulation of nucleic acid binding. Cryo-electron microscopy, image classification Nucleic acids research High 23002137
2012 The TTT-RUVBL1/2 complex responds to the cell's metabolic state (ATP levels from glucose/glutamine metabolism), is disassembled upon energy depletion, and is required for mTORC1 assembly as obligate dimers, for mTORC1-Rag interaction, and for lysosomal localization of mTORC1, acting via an AMPK-, TSC1/2-, and Rag-independent mechanism. Co-immunoprecipitation, siRNA knockdown, mTORC1 activity assays, lysosomal fractionation, metabolic perturbation Molecular cell High 23142078
2012 Crystal structure of TIP49b (RUVBL2) hexamer and molecular dynamics simulations established that domain mobility affects ATPase activity, and hexamerization itself downregulates ATPase activity. A three-amino acid insertion in the lik-TIP49b mutant remotely affects the ATP-binding/hydrolysis pocket by altering conformational dynamics, uncoupling ATP hydrolysis from DNA binding. X-ray crystallography, molecular dynamics simulation, ATPase assay Structure High 22748767
2013 RuvbL1 and RuvbL2 associate with the aggresome, act as protein disaggregases: polypeptides with unfolded structures and amyloid fibrils stimulate their ATPase activity, and RuvbL complexes actively disassemble protein aggregates. The aggresome substrate synphilin-1 interacts directly with the RuvbL1 barrel-like structure near the central channel opening. siRNA knockdown, aggresome formation assay, ATPase activity assay, protein disaggregation assay, direct protein interaction assay The EMBO journal High 26303906
2014 YY1 oligomers bind RuvBL1-RuvBL2 hetero-oligomeric complexes preferentially through RuvBL1. DNA binding by YY1 oligomers is enhanced by RuvBL1-RuvBL2, and the ATPase activity of RuvBL2 and YY1 are both required for RAD51 foci formation during homologous recombination. Electron microscopy, pulldown, co-immunoprecipitation, bimolecular fluorescence complementation, RAD51 foci assay, ATPase-deficient mutants The Journal of biological chemistry Medium 24990942
2014 RuvBL1 and RuvBL2 co-purify with the Fanconi anemia (FA) core complex under native conditions. Depletion of RuvBL1-RuvBL2 causes hallmark FA phenotypes (crosslinker sensitivity, chromosomal instability, defective FA pathway activation) and reduces FA core complex abundance in human cells. Genetic knockout of RuvBL1 in mice is embryonic lethal; conditional hematopoietic KO causes aplastic anemia. Native complex purification and mass spectrometry, siRNA depletion, crosslinker sensitivity assay, conditional mouse KO Nucleic acids research High 25428364
2014 Cytoplasmic RUVBL1 directly binds filamentous actin (F-actin) in cell protrusions, increases local G-actin concentration, promotes peripheral actin polymerization, and thereby induces formation of membrane protrusions that enhance pancreatic cancer cell invasion. siRNA knockdown, co-immunoprecipitation with actin, confocal immunofluorescence, invasion assay International journal of oncology Medium 24728183
2015 RUVBL1 localizes to structures of the mitotic spindle apparatus in anaphase-to-telophase, partially co-localizing with PLK1. PLK1 phosphorylates RUVBL1 (but not RUVBL2) in vitro and physically associates with RUVBL1 in vivo. siRNA knockdown of RUVBL1 or RUVBL2 causes severe chromosome misalignment and missegregation. RUVBL1 ATPase activity is indispensable for cell proliferation. Immunofluorescence microscopy, in vitro kinase assay (PLK1 on RUVBL1), co-immunoprecipitation, siRNA knockdown, ATPase-deficient mutant PloS one High 26201077
2015 ECD (Ecdysoneless) protein directly interacts with RUVBL1 component of the R2TP co-chaperone complex in a phosphorylation-independent manner (distinct from PIH1D1 interaction), and this RUVBL1-ECD interaction is essential for ECD's cell cycle progression function. Co-immunoprecipitation, GST pulldown, phosphorylation mutant analysis, cell cycle rescue assay Molecular and cellular biology Medium 26711270
2017 PRMT5 methylates RUVBL1 at arginine R205, which is required for TIP60-dependent histone H4K16 acetylation, 53BP1 displacement from DNA double-strand breaks, and promotion of homologous recombination. RUVBL1 methylation by PRMT5 is critically required for TIP60 acetyltransferase activity but does not affect ATM activation by TIP60. In vitro methylation assay (PRMT5 on RUVBL1), HAT activity assay, ChIP, immunofluorescence for 53BP1/RAD51 foci, site-directed mutagenesis (R205A) Molecular cell High 28238654
2017 An Ino80 ATPase domain (Ino80INS) stimulates yeast Rvb1/Rvb2 ATPase activity by 16-fold while promoting dodecamerization of the Rvbs. Ino80INS binds asymmetrically along the dodecamerization interface, resulting in a conformationally flexible dodecamer that collapses into hexamers upon ATP addition, demonstrating chaperone-like protein assembly behavior. In vitro ATPase assay, cryo-EM, mass spectrometry, integrative structural modeling Cell reports High 28591576
2017 RUVBL1-RUVBL2 directly interacts with ZNHIT2 to mediate R2TP/Prefoldin-like complex association with U5 snRNP; the zinc-finger HIT domain of ZNHIT2 binds RUVBL2. Disruption of ZNHIT2 and RUVBL2 expression impacts U5 snRNP protein composition, suggesting R2TP/PFDL promotes U5 snRNP assembly. Affinity purification-mass spectrometry, co-immunoprecipitation, siRNA knockdown, native PAGE analysis of snRNP composition Nature communications High 28561026
2017 Adenovirus E1A suppresses type I interferon-stimulated gene expression by binding to RUVBL1 via its C-terminus and being recruited to RUVBL1-regulated promoters in an interferon-dependent manner, thereby preventing their activation. Co-immunoprecipitation (E1A-RUVBL1), ChIP, reporter gene assay, siRNA knockdown of RUVBL1 Journal of virology Medium 28122980
2018 RUVBL1 interacts with DNAAF1 (dynein arm assembly factor) and IFT88 (intraflagellar transport protein); loss of RUVBL1 perturbs co-localization of DNAAF1 with IFT88. RUVBL1 orthologs show asymmetric left-sided distribution at the mouse embryonic node and zebrafish Kupffer's vesicle (dependent on DNAAF1), linking RUVBL1 to symmetry breaking and cardiac development. Co-immunoprecipitation, co-localization imaging, siRNA knockdown, zebrafish complementation assay Human molecular genetics Medium 29228333
2018 Conditional deletion of Ruvbl1 in renal tubular epithelial cells causes renal failure with tubular dilatations and fewer ciliated cells. Inducible deletion in cells with motile cilia causes hydrocephalus. Cilia of Ruvbl1-negative cells lack crucial ciliary proteins, consistent with RUVBL1-dependent cytoplasmic pre-assembly of ciliary protein complexes. Conditional mouse knockout, histology, immunofluorescence Experimental & molecular medicine High 29959317
2018 RUVBL1 activates the RAF/MEK/ERK pathway by binding C-RAF and inhibiting phosphorylation of C-RAF at serine 259, thereby promoting lung cancer cell proliferation and invasion. Co-immunoprecipitation (RUVBL1-C-RAF), Western blot for pS259-C-RAF, siRNA/CRISPR knockout, xenograft tumor model Biochemical and biophysical research communications Medium 29545175
2019 CB-6644, an allosteric small-molecule inhibitor of RUVBL1/2 ATPase activity, specifically engages RUVBL1/2 in cancer cells. Drug-resistant cell clones contain amino acid mutations in RUVBL1 or RUVBL2, confirming on-target cell killing. CB-6644 significantly reduces tumor growth in AML and multiple myeloma xenograft models. ATPase activity assay, resistance mutation mapping, xenograft mouse models ACS chemical biology High 30640450
2019 Cryo-EM structures of the human R2TP complex (RUVBL1-RUVBL2-RPAP3-PIH1D1) revealed that PIH1D1 binding to the DII domain of RUVBL2 induces conformational rearrangements that destabilize an N-terminal segment of RUVBL2 acting as a gatekeeper to nucleotide exchange, thereby regulating ATPase activity of the complex. Cryo-EM structural analysis Science advances High 31049401
2019 RUVBL1/2 ATPase activity is necessary for maturation or dissociation of the PAQosome (large RUVBL1/2-dependent multiprotein complex) and for DNA replication; inhibition of ATPase activity causes S-phase arrest and replication catastrophe in cancer cells. ATPase inhibitor (CB-6644) treatment, flow cytometry (S-phase arrest), native PAGE (PAQosome integrity) Cell chemical biology High 31883965
2019 RUVBL1 recruits to the Tip60 complex, and the RUVBL1-β-catenin/LEF1-TIP60 complex promotes histone H4K16 acetylation near the C-myc promoter, enhancing chromatin remodeling and transcriptional activity. Co-immunoprecipitation, chromatin immunoprecipitation, histone H4K16Ac assay, shRNA knockdown Molecular cancer Medium 31665067
2020 DHX34, an RNA helicase regulating NMD initiation, directly interacts with RUVBL1-RUVBL2. Cryo-EM shows DHX34 induces extensive changes in the N-termini of every RUVBL2 subunit, stabilizing a conformation that does not bind nucleotide and thereby down-regulates ATP hydrolysis; DHX34 acts exclusively on RUVBL2 subunits. This couples RUVBL ATPase activity to NMD initiation complex assembly. Cryo-EM, in vitro binding assay, ATPase assay, ATPase-deficient mutants, co-immunoprecipitation eLife High 33205750
2020 RUVBL1-RUVBL2 complex controls assembly and composition of the γ-tubulin ring complex (γTuRC) in human cells; RUVBL interacts with γTuRC subcomplexes but is not part of fully assembled γTuRC. In a heterologous coexpression system, RUVBL can assemble γTuRC from minimal core subunits; reconstituted γTuRC has nucleation activity. siRNA knockdown, coexpression reconstitution, cryo-EM (γTuRC structure at ~4.0 Å), microtubule nucleation assay, co-immunoprecipitation Science advances High 33355144
2020 Both overexpression and depletion of RUVBL1 impede DNA replication fork rates and cause accumulation of phospho-H2AX in a transcription-dependent manner. RUVBL1 overexpression enhances c-Myc-dependent RNAPII pause release and increases global transcription; RUVBL1 depletion increases ubiquitination of RNAPII (Rpb1) and reduces its chromatin mobility, indicating stalled RNAPII. DNA fiber assay (replication fork rate), γH2AX immunofluorescence, RNAPII ChIP (phospho-Ser2 CTD), transcription inhibition rescue The international journal of biochemistry & cell biology Medium 32846207
2021 NOPCHAP1 (C12ORF45) acts as a bridge between NOP58 and the PAQosome: it makes direct physical interactions with the CC-NOP domain of NOP58 and domain II of RUVBL1/2 AAA+ ATPases. NOPCHAP1 interaction with RUVBL1/2 is disrupted upon ATP binding. NOPCHAP1 KO cells show decreased NOP58 expression, establishing NOPCHAP1 as a client-loading cofactor selecting NOP58 for RUVBL1/2-mediated box C/D snoRNP assembly. Co-immunoprecipitation, NOP58 mutant analysis, mass spectrometry proteomics, KO cell analysis Nucleic acids research High 33367824
2021 RUVBL1 interacts with SMCHD1 and is present at D4Z4 chromatin in myocytes; loss of RUVBL1 further derepresses DUX4 expression in FSHD myocytes, establishing RUVBL1 as a functional component of SMCHD1-mediated D4Z4 chromatin repression. Quantitative proteomics (SMCHD1 pulldown), ChIP-seq, siRNA knockdown, DUX4 expression assay Scientific reports Medium 34880314
2021 RUVBL1/2 inhibition significantly reduces histone H3K4me3 at promoters of pro-inflammatory genes (Nos2, Il6) and diminishes NF-κB recruitment to corresponding enhancers, establishing RUVBL1/2 as regulators of macrophage pro-inflammatory responses via epigenetic (H3K4me3) mechanisms. siRNA knockdown, pharmacological inhibition (CB-6644), ChIP for H3K4me3, NF-κB ChIP, nitric oxide assay Frontiers in immunology Medium 34276666
2022 RUVBL1 promotes enzalutamide resistance by accumulating in the cytoplasm, where it enhances recruitment of CRAF to PLXNA1 and activates downstream MAPK signaling as an AR-independent survival pathway. Co-immunoprecipitation (RUVBL1-CRAF-PLXNA1), siRNA/inhibitor experiments, xenograft models Oncogene Medium 35508542
2022 Yeast Rvb1/Rvb2 couple transcription and mRNA fate: they enrich at promoters and mRNAs of alternative glucose metabolism genes during starvation. Engineered Rvb tethering to mRNAs is sufficient to sequester mRNAs into granules, repress translation, and drive further transcriptional upregulation of target genes. ChIP-seq, mRNA-seq, RNA immunoprecipitation, engineered tethering system, mRNP granule assay eLife Medium 36107469
2022 LINC00839 (lncRNA) recruits RUVBL1 to the TIP60 complex, increases its acetyltransferase activity, and guides the complex to the NRF1 promoter, promoting H4K5 and H4K8 acetylation and NRF1 transcription to activate mitochondrial metabolism. Co-immunoprecipitation, ChIP, HAT activity assay, RNA pulldown, siRNA knockdown EMBO reports Medium 35876654
2023 GART methylates RUVBL1 at lysine K7, enhancing RUVBL1 stability and thereby aberrantly activating the Wnt/β-catenin signaling pathway to induce tumor stemness; GART methyltransferase activity is centered on the E948 site. In vitro methylation assay, site-directed mutagenesis (GART E948, RUVBL1 K7), co-immunoprecipitation, β-catenin reporter assay Advanced science Medium 37439412
2023 RUVBL1 controls MYC chromatin binding and modulates MYC-driven EEF1A1 expression, thereby regulating protein synthesis in Ewing sarcoma cells. A high-density CRISPR gene body scan identified the critical MYC-interacting residue in RUVBL1. CRISPR screens, ChIP-seq (MYC), ribosome profiling, CRISPR gene body scan, siRNA knockdown Advanced science Medium 37075745
2024 DTL (CDT2/DCAF2) ubiquitinates RUVBL1, facilitating its binding to RUVBL2 and β-catenin while attenuating its binding to TIP60. Ubiquitinated RUVBL1 promotes transcriptional regulation of NHEJ repair pathway genes and attenuates TIP60-mediated H4K16 acetylation and homologous recombination repair, thereby enhancing radioresistance. Co-immunoprecipitation, ubiquitination assay, HAT assay (H4K16Ac), reporter gene assay, siRNA/overexpression Cell death & disease Medium 38609375
2024 RUVBL1/2 interact with YTHDF1 at 40S translation initiation complexes; YTHDF1 binds m6A-modified RUVBL1/2 mRNA to promote their translation. RUVBL1/2 depletion stalls YTHDF1-driven oncogenic translation and impairs MAPK, RAS, and PI3K-AKT signaling in colorectal cancer. Co-immunoprecipitation, mass spectrometry, ribosome sequencing, CRISPR screening, m6A analysis Cancer research Medium 38900944
2024 RUVBL1 is required for MYC-driven oncogenic and immunoevasive gene expression in pancreatic cancer. Auxin-degron degradation of RUVBL1 leads to complete tumor regression in mice, preceded by immune cell infiltration, establishing RUVBL1 as a nuclear effector of MYC transcriptional programs. Auxin-degron system for rapid protein degradation, shRNA library screens in vitro and in vivo, RNA-seq, xenograft models Gut High 38821858

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity. The EMBO journal 238 11080158
2009 RVB1/RVB2: running rings around molecular biology. Molecular cell 192 19524533
2012 Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Molecular cell 185 23142078
1998 Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proceedings of the National Academy of Sciences of the United States of America 153 9843967
2006 Crystal structure of the human AAA+ protein RuvBL1. The Journal of biological chemistry 136 17060327
2010 AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Science signaling 130 20371770
2008 Human Rvb1/Tip49 is required for the histone acetyltransferase activity of Tip60/NuA4 and for the downregulation of phosphorylation on H2AX after DNA damage. Molecular and cellular biology 130 18285460
2017 PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Molecular cell 125 28238654
1999 TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. The Journal of biological chemistry 122 10428817
1998 An eukaryotic RuvB-like protein (RUVBL1) essential for growth. The Journal of biological chemistry 116 9774387
2019 CircMYO10 promotes osteosarcoma progression by regulating miR-370-3p/RUVBL1 axis to enhance the transcriptional activity of β-catenin/LEF1 complex via effects on chromatin remodeling. Molecular cancer 112 31665067
2011 Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex. Journal of structural biology 104 21933716
1997 Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB. Biochemical and biophysical research communications 100 9196036
2017 R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nature communications 94 28561026
2003 TIP49 regulates beta-catenin-mediated neoplastic transformation and T-cell factor target gene induction via effects on chromatin remodeling. Cancer research 90 14695187
2005 Myc interacts genetically with Tip48/Reptin and Tip49/Pontin to control growth and proliferation during Drosophila development. Proceedings of the National Academy of Sciences of the United States of America 86 16087886
2006 Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. Journal of molecular biology 85 17157868
2014 Autoantibodies to RuvBL1 and RuvBL2: a novel systemic sclerosis-related antibody associated with diffuse cutaneous and skeletal muscle involvement. Arthritis care & research 84 24023044
2013 Chaperone-like activity of the AAA+ proteins Rvb1 and Rvb2 in the assembly of various complexes. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 82 23530256
2008 Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex. Journal of molecular biology 76 18234224
2002 TIP49, but not TRRAP, modulates c-Myc and E2F1 dependent apoptosis. Oncogene 72 12185582
1999 A rat RuvB-like protein, TIP49a, is a germ cell-enriched novel DNA helicase. The Journal of biological chemistry 72 10336418
2019 CB-6644 Is a Selective Inhibitor of the RUVBL1/2 Complex with Anticancer Activity. ACS chemical biology 66 30640450
2020 RUVBL1-RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Current opinion in structural biology 60 33129013
2010 Rvb1-Rvb2: essential ATP-dependent helicases for critical complexes. Biochemistry and cell biology = Biochimie et biologie cellulaire 60 20130677
2003 The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. Cell motility and the cytoskeleton 60 14506706
2001 Purification, cloning, and characterization of a profibrinolytic plasminogen-binding protein, TIP49a. The Journal of biological chemistry 57 11027681
2019 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell chemical biology 56 31883965
2009 Cooperative action of TIP48 and TIP49 in H2A.Z exchange catalyzed by acetylation of nucleosomal H2A. Nucleic acids research 54 19696079
2012 Conformational transitions regulate the exposure of a DNA-binding domain in the RuvBL1-RuvBL2 complex. Nucleic acids research 51 23002137
2018 Presence of anti-eukaryotic initiation factor-2B, anti-RuvBL1/2 and anti-synthetase antibodies in patients with anti-nuclear antibody negative systemic sclerosis. Rheumatology (Oxford, England) 47 29294089
2015 RuvbL1 and RuvbL2 enhance aggresome formation and disaggregate amyloid fibrils. The EMBO journal 47 26303906
2020 Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Science advances 46 33355144
2022 LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1. EMBO reports 43 35876654
2017 Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones. Cell reports 43 28591576
2014 Structure of Yin Yang 1 oligomers that cooperate with RuvBL1-RuvBL2 ATPases. The Journal of biological chemistry 43 24990942
1998 TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human. Biochemical and biophysical research communications 42 9588198
2019 Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM. Science advances 39 31049401
2010 Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes. The Biochemical journal 39 20412048
2011 Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex. Cancer science 38 21951644
2010 Comparison of the multiple oligomeric structures observed for the Rvb1 and Rvb2 proteins. Biochemistry and cell biology = Biochimie et biologie cellulaire 38 20130681
2008 RAD51 foci formation in response to DNA damage is modulated by TIP49. The international journal of biochemistry & cell biology 37 18834951
2011 A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis. EMBO reports 36 21617703
2014 Abundance of the Fanconi anaemia core complex is regulated by the RuvBL1 and RuvBL2 AAA+ ATPases. Nucleic acids research 35 25428364
2012 Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2. Nucleus (Austin, Tex.) 35 22540023
2018 DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport. Human molecular genetics 29 29228333
2012 Large-scale conformational flexibility determines the properties of AAA+ TIP49 ATPases. Structure (London, England : 1993) 29 22748767
2015 Chromosome Missegregation Associated with RUVBL1 Deficiency. PloS one 28 26201077
2023 GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 27 37439412
2003 Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a. Molecular cancer 26 14675489
2015 A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Molecular and cellular biology 25 26711270
1999 Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins. Biochimica et biophysica acta 25 10524211
2023 Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 24 37075745
2022 RUVBL1 promotes enzalutamide resistance of prostate tumors through the PLXNA1-CRAF-MAPK pathway. Oncogene 24 35508542
2018 Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus. Experimental & molecular medicine 23 29959317
2014 RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. International journal of oncology 23 24728183
2020 Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells. Science advances 22 32789167
2019 Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. Viruses 22 31018511
2017 Pih1p-Tah1p Puts a Lid on Hexameric AAA+ ATPases Rvb1/2p. Structure (London, England : 1993) 21 28919439
2016 Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 21 27722820
1999 A notable example of an evolutionary conserved gene: studies on a putative DNA helicase TIP49. DNA sequence : the journal of DNA sequencing and mapping 21 10565543
2019 The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis. The Plant journal : for cell and molecular biology 20 30834599
2017 RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer. Biochimica et biophysica acta. General subjects 20 28341484
2021 NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis. Nucleic acids research 18 33367824
2015 Yeast rvb1 and rvb2 proteins oligomerize as a conformationally variable dodecamer with low frequency. Journal of molecular biology 18 25636407
2019 Liver haploinsufficiency of RuvBL1 causes hepatic insulin resistance and enhances hepatocellular carcinoma progression. International journal of cancer 17 31721195
2022 Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling. Theranostics 16 36438486
2018 RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis. Biochemical and biophysical research communications 16 29545175
2017 Suppression of Type I Interferon Signaling by E1A via RuvBL1/Pontin. Journal of virology 16 28122980
2024 RUVBL1 ubiquitination by DTL promotes RUVBL1/2-β-catenin-mediated transcriptional regulation of NHEJ pathway and enhances radiation resistance in breast cancer. Cell death & disease 15 38609375
2020 Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. eLife 14 33205750
2023 Combined CRISPRi and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression. American journal of human genetics 13 37541187
2021 RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation. Frontiers in immunology 13 34276666
2018 Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila. Cell death discovery 13 30062057
2000 Expression of Xenopus homologs of the beta-catenin binding protein pontin52. Mechanisms of development 13 10842076
2024 RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis. Cancer research 12 38900944
2022 Involvement of RUVBL1 in WNT/β-Catenin Signaling in Oral Squamous Cell Carcinoma. Disease markers 12 35493294
2020 RUVBL1 is an amplified epigenetic factor promoting proliferation and inhibiting differentiation program in head and neck squamous cancers. Oral oncology 12 32745900
2016 C-FLIPL Modulated Wnt/β-Catenin Activation via Association with TIP49 Protein. The Journal of biological chemistry 12 28028178
2024 Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2. Gut 11 38821858
2022 Discovery of small-molecule inhibitors of RUVBL1/2 ATPase. Bioorganic & medicinal chemistry 11 35364523
2016 Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components. Molecular & cellular proteomics : MCP 11 26831523
2023 RUVBL1-modulated chromatin remodeling alters the transcriptional activity of oncogenic CTNNB1 in uveal melanoma. Cell death discovery 8 37076452
2021 RuvBL1 Maintains Resistance to TRAIL-Induced Apoptosis by Suppressing c-Jun/AP-1 Activity in Non-Small Cell Lung Cancer. Frontiers in oncology 8 34164343
2016 The Relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in Benign and Malignant Human Lymphoid Tissues. Biochemistry and biophysics reports 8 27066592
2024 The Lyn/RUVBL1 Complex Promotes Colorectal Cancer Liver Metastasis by Regulating Arachidonic Acid Metabolism Through Chromatin Remodeling. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 7 39665272
2023 EIF3D promotes resistance to 5-fluorouracil in colorectal cancer through upregulating RUVBL1. Journal of clinical laboratory analysis 7 36592991
2022 Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. Journal of molecular biology 7 35901867
2008 Cloning, expression, purification, crystallization and preliminary X-ray analysis of the human RuvBL1-RuvBL2 complex. Acta crystallographica. Section F, Structural biology and crystallization communications 7 18765919
2007 Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues. FEMS yeast research 7 17302941
2023 Downregulation of AHNAK2 inhibits cell cycle of lung adenocarcinoma cells by interacting with RUVBL1. Thoracic cancer 6 37349884
2022 Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation. eLife 6 36107469
2020 Deregulated levels of RUVBL1 induce transcription-dependent replication stress. The international journal of biochemistry & cell biology 6 32846207
2017 Human DNA helicase, RuvBL1 and its Chlamydomonas homologue, CrRuvBL1 plays an important role in ciliogenesis. Cytoskeleton (Hoboken, N.J.) 6 28574207
2023 IRX2 regulates endometrial carcinoma oncogenesis by transcriptional repressing RUVBL1. Experimental cell research 4 38042247
2021 Leishmania major RUVBL1 has a hexameric conformation in solution and, in the presence of RUVBL2, forms a heterodimer with ATPase activity. Archives of biochemistry and biophysics 4 33775623
2021 A proteomics study identifying interactors of the FSHD2 gene product SMCHD1 reveals RUVBL1-dependent DUX4 repression. Scientific reports 4 34880314
2005 Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1. Acta crystallographica. Section F, Structural biology and crystallization communications 4 16511264
2000 Chromosome mapping and expression of human tip49 family genes. DNA sequence : the journal of DNA sequencing and mapping 4 10902922
2024 Molecular Signatures of CB-6644 Inhibition of the RUVBL1/2 Complex in Multiple Myeloma. International journal of molecular sciences 3 39201707