Affinage

RSL1D1

Ribosomal L1 domain-containing protein 1 · UniProt O76021

Round 2 corrected
Length
490 aa
Mass
55.0 kDa
Annotated
2026-04-28
130 papers in source corpus 5 papers cited in narrative 5 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RSL1D1 (also known as CSIG) is a nucleolar ribosomal L1 domain-containing protein that functions as a multifaceted extra-ribosomal regulator of cell proliferation, senescence, and stress responses. It suppresses PTEN translation by binding the PTEN mRNA 5′ UTR, thereby promoting cell cycle progression and delaying replicative senescence (PMID:18678645), and stabilizes c-MYC protein by reducing its ubiquitination (PMID:25749381). Under nucleolar stress, RSL1D1 translocates to the nucleoplasm where it binds the MDM2 RING finger domain, inhibits MDM2 E3 ligase activity, and stabilizes p53 to enforce G1 arrest (PMID:27811966). RSL1D1 also suppresses autophagy by interacting with RAN GTPase and competitively blocking SIRT7-mediated RAN deacetylation, which prevents nuclear STAT3 accumulation and STAT3-driven autophagic gene expression (PMID:35013134).

Mechanistic history

Synthesis pass · year-by-year structured walk · 5 steps
  1. 2008 High

    Establishing that RSL1D1 has an extra-ribosomal function: it directly binds the PTEN mRNA 5′ UTR to suppress PTEN translation, linking a nucleolar protein to translational control of a tumor suppressor and replicative senescence.

    Evidence shRNA knockdown, luciferase 5′ UTR reporter assay, RNA immunoprecipitation, and epistasis in PTEN-null cells in human fibroblasts

    PMID:18678645

    Open questions at the time
    • Structural basis of RSL1D1–PTEN mRNA interaction is undefined
    • Whether RSL1D1 regulates other mRNAs beyond PTEN is unexplored
    • Upstream signals controlling RSL1D1 expression during senescence are unknown
  2. 2012 Medium

    Revealing that RSL1D1 participates in UV-induced apoptosis by serving as a nucleolar effector of p33ING1, which stabilizes RSL1D1 protein to activate Bax-mediated cell death.

    Evidence Co-immunoprecipitation with domain-deletion mutants, UV irradiation, immunofluorescence, and apoptosis marker analysis

    PMID:22419112

    Open questions at the time
    • Mechanism by which RSL1D1 activates Bax is not defined
    • Single-lab finding without independent replication
    • Whether this pathway operates in non-UV stress contexts is untested
  3. 2015 Medium

    Demonstrating that RSL1D1 stabilizes c-MYC oncoprotein by reducing its ubiquitination, establishing RSL1D1 as a post-translational regulator of a second major oncogenic node beyond PTEN.

    Evidence Co-immunoprecipitation, ubiquitination assay, shRNA knockdown in hepatocellular carcinoma cells, and xenograft mouse model

    PMID:25749381

    Open questions at the time
    • The E3 ligase whose activity on c-MYC is antagonized by RSL1D1 is not identified
    • Single-lab observation without independent confirmation
    • Direct versus indirect effect on c-MYC ubiquitination is unresolved
  4. 2016 High

    Showing that nucleolar stress triggers RSL1D1 translocation to the nucleoplasm where it directly inhibits MDM2 E3 ligase activity via the RING finger domain, stabilizing p53 — establishing RSL1D1 as a nucleolar stress sensor coupling ribosome biogenesis surveillance to the p53 checkpoint.

    Evidence In vitro ubiquitination assay, co-immunoprecipitation mapping the MDM2 RING domain, immunofluorescence documenting nucleolar-to-nucleoplasmic translocation, and shRNA knockdown with cell cycle analysis

    PMID:27811966

    Open questions at the time
    • Signal that triggers RSL1D1 release from the nucleolus is unknown
    • Whether RSL1D1 competes with ribosomal proteins (e.g. RPL5/RPL11) for MDM2 binding is untested
    • Structural details of the RSL1D1–MDM2 RING interaction are lacking
  5. 2022 Medium

    Uncovering an autophagy-suppressive role: RSL1D1 binds RAN GTPase and competitively blocks SIRT7-mediated deacetylation, keeping RAN acetylated to inhibit nuclear STAT3 accumulation and autophagic gene expression — connecting RSL1D1 to nucleocytoplasmic transport regulation.

    Evidence Co-immunoprecipitation, competitive binding assay (RSL1D1 vs SIRT7 for RAN), acetylation assays, nuclear fractionation, autophagy flux assays in colorectal cancer cells, and in vivo xenograft

    PMID:35013134

    Open questions at the time
    • Whether RSL1D1–RAN interaction is GTP/GDP-dependent is unknown
    • Single-lab finding; independent validation is needed
    • How RSL1D1's autophagy-suppressive function integrates with its p53-stabilizing role under stress is unexplored

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RSL1D1's multiple functions — translational suppression of PTEN, c-MYC stabilization, MDM2 inhibition, and RAN-dependent autophagy suppression — are coordinated or prioritized in different cellular contexts remains undefined.
  • No structural model of RSL1D1 or its RNA-binding interface exists
  • Physiological roles in normal tissue homeostasis (beyond cancer lines) are unstudied
  • Whether RSL1D1 participates in ribosome biogenesis as a structural component or only in extra-ribosomal signaling is unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 3 GO:0003723 RNA binding 1 GO:0045182 translation regulator activity 1
Localization
GO:0005730 nucleolus 3 GO:0005654 nucleoplasm 1
Pathway
R-HSA-1640170 Cell Cycle 2 R-HSA-5357801 Programmed Cell Death 2 GO:0005634 nucleus 1 R-HSA-9612973 Autophagy 1
Partners
ING1MDM2MYCRAN

Evidence

Reading pass · 5 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 CSIG (RSL1D1) inhibits PTEN translation via direct interaction with the PTEN mRNA 5' UTR, thereby reducing PTEN protein levels and promoting cell proliferation. Knockdown of CSIG increased luciferase activity from a PTEN 5'UTR-reporter, confirming translational suppression. CSIG also negatively regulates p27(Kip1) in a PTEN-dependent manner. Overexpression of CSIG delayed replicative senescence, while knockdown accelerated it. shRNA knockdown, luciferase 5'UTR reporter assay, RNA immunoprecipitation (RIP), Western blot, cell cycle analysis, PTEN-deficient cell lines as epistasis controls Molecular and cellular biology High 18678645
2012 CSIG (RSL1D1) is required for p33ING1-mediated apoptosis in response to UV irradiation. After UV, p33ING1 translocates to the nucleolus, binds CSIG via its nucleolar targeting sequence, and stabilizes CSIG protein, which in turn activates Bax to promote apoptosis. CSIG localizes to the nucleolus and is upregulated by UV irradiation. Co-immunoprecipitation, domain-deletion mutants, UV irradiation assay, immunofluorescence/subcellular localization, Western blot for Bax and apoptosis markers Cell death & disease Medium 22419112
2015 CSIG (RSL1D1) directly interacts with c-MYC protein and stabilizes c-MYC by reducing its ubiquitination and proteasomal degradation, thereby promoting hepatocellular carcinoma cell proliferation. Knockdown of CSIG increased c-MYC ubiquitination and reduced c-MYC protein levels. Co-immunoprecipitation, ubiquitination assay, shRNA knockdown, xenograft mouse model, Western blot Oncotarget Medium 25749381
2016 CSIG (RSL1D1) translocates from the nucleolus to the nucleoplasm in response to nucleolar stress and directly interacts with the MDM2 RING finger domain, inhibiting MDM2 E3 ubiquitin ligase activity. This results in decreased MDM2-mediated p53 ubiquitination and degradation, leading to p53 stabilization and G1 arrest. Knockdown of CSIG attenuated p53 induction and G1 arrest under nucleolar stress. Co-immunoprecipitation, in vitro ubiquitination assay, immunofluorescence (nucleolar-to-nucleoplasmic translocation), shRNA knockdown, cell cycle analysis Scientific reports High 27811966
2022 RSL1D1 promotes colorectal cancer progression by suppressing autophagy through a RAN/STAT3 axis. RSL1D1 interacts with RAN GTPase and competitively inhibits its deacetylation by SIRT7, thereby keeping RAN in an acetylated state. Acetylated RAN inhibits nuclear STAT3 accumulation and STAT3-regulated autophagic gene expression, suppressing autophagy and promoting tumor cell proliferation, invasion, and metastasis. Co-immunoprecipitation, competitive binding assay (CSIG vs SIRT7 for RAN), acetylation assay, nuclear fractionation, autophagy flux assay, shRNA knockdown/overexpression in CRC cells, in vivo xenograft Cell death & disease Medium 35013134

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2006 A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nature biotechnology 1336 16964243
2016 ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 1233 26777405
2008 Identification of host proteins required for HIV infection through a functional genomic screen. Science (New York, N.Y.) 1165 18187620
2004 Large-scale characterization of HeLa cell nuclear phosphoproteins. Proceedings of the National Academy of Sciences of the United States of America 1159 15302935
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2020 Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Science (New York, N.Y.) 564 33060197
2005 Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell 544 16213212
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2002 Functional proteomic analysis of human nucleolus. Molecular biology of the cell 391 12429849
2015 Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry. Nature methods 370 26414014
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
1987 Structure of the C-terminal domain of the ribosomal protein L7/L12 from Escherichia coli at 1.7 A. Journal of molecular biology 189 3309338
1996 Immunization of mice with recombinant L7/L12 ribosomal protein confers protection against Brucella abortus infection. Vaccine 136 8873388
2012 L1/2 regularization: a thresholding representation theory and a fast solver. IEEE transactions on neural networks and learning systems 126 24807129
2002 Production and targeting of the Brucella abortus antigen L7/L12 in Lactococcus lactis: a first step towards food-grade live vaccines against brucellosis. Applied and environmental microbiology 114 11823235
2006 The ribosomal stalk binds to translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal domain. Journal of molecular biology 105 17070545
2004 From structure and dynamics of protein L7/L12 to molecular switching in ribosome. The Journal of biological chemistry 93 14960595
1985 A molecular dynamics study of the C-terminal fragment of the L7/L12 ribosomal protein. Secondary structure motion in a 150 picosecond trajectory. Journal of molecular biology 88 3894675
1978 Oxidation of the methionine residues of Escherichia coli ribosomal protein L12 decreases the protein's biological activity. Proceedings of the National Academy of Sciences of the United States of America 84 364476
2006 Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice. Infection and immunity 83 16622210
2000 Flexibility, conformational diversity and two dimerization modes in complexes of ribosomal protein L12. The EMBO journal 81 10637222
1993 Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre. Journal of molecular biology 77 8263910
2005 Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM. Molecular cell 74 16337596
1978 Dimer state of protein L7/L12 and EF-G-dependent reactions of ribosomes. European journal of biochemistry 71 361401
2011 Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription. Proceedings of the National Academy of Sciences of the United States of America 70 22003127
1976 Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39. The Journal of biological chemistry 70 1002715
1982 Autogenous control: ribosomal protein L10-L12 complex binds to the leader sequence of its mRNA. The EMBO journal 68 6765237
1985 Involvement of ribosomal protein L7/L12 in control of translational accuracy. Proceedings of the National Academy of Sciences of the United States of America 67 3883346
1999 Replacement of L7/L12.L10 protein complex in Escherichia coli ribosomes with the eukaryotic counterpart changes the specificity of elongation factor binding. The Journal of biological chemistry 65 10488095
1997 Identification of functionally important regions of the muscular chloride channel CIC-1 by analysis of recessive and dominant myotonic mutations. Human molecular genetics 65 9158157
2013 Sparse logistic regression with a L1/2 penalty for gene selection in cancer classification. BMC bioinformatics 64 23777239
2004 Conformation and dynamics of ribosomal stalk protein L12 in solution and on the ribosome. Biochemistry 64 15147176
1974 Alteration in the acetylation level of ribosomal protein L12 during growth cycle of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 64 4600787
1980 Ribosomal protein L7/L12 is required for optimal translation. Proceedings of the National Academy of Sciences of the United States of America 62 7001453
1992 Direct evidence for interaction of the conserved GTPase domain within 28 S RNA with mammalian ribosomal acidic phosphoproteins and L12. The Journal of biological chemistry 59 1527039
1997 Nucleic acid vaccination of Brucella abortus ribosomal L7/L12 gene elicits immune response. Vaccine 57 9413093
1997 The L7/L12 ribosomal domain of the ribosome: structural and functional studies. FEBS letters 56 9175862
1994 Recombinant L7/L12 ribosomal protein and gamma-irradiated Brucella abortus induce a T-helper 1 subset response from murine CD4+ T cells. Immunology 56 7875746
2008 Acetylation of L12 increases interactions in the Escherichia coli ribosomal stalk complex. Journal of molecular biology 54 18514735
1998 The GTPase center protein L12 is required for correct ribosomal stalk assembly but not for Saccharomyces cerevisiae viability. The Journal of biological chemistry 53 9822666
1986 Localization of two epitopes of protein L7/L12 to both the body and stalk of the large ribosomal subunit. Immune electron microscopy using monoclonal antibodies. The Journal of biological chemistry 53 2422173
1996 Tetramethylrhodamine dimer formation as a spectroscopic probe of the conformation of Escherichia coli ribosomal protein L7/L12 dimers. The Journal of biological chemistry 52 8631789
2002 Yeast ribosomal protein L12 is a substrate of protein-arginine methyltransferase 2. The Journal of biological chemistry 51 11856739
2002 Ribosomal protein L12 uses a distinct nuclear import pathway mediated by importin 11. Molecular and cellular biology 48 11809816
2007 Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. Vaccine 47 17296251
1988 Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functions. Biochimie 47 3139080
2000 Role of ribosomal protein L12 in gonococcal invasion of Hec1B cells. Infection and immunity 44 10948117
2015 Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. The Journal of biological chemistry 43 26586915
2009 Protection of mice from Brucella infection by immunization with attenuated Salmonella enterica serovar typhimurium expressing A L7/L12 and BLS fusion antigen of Brucella. Vaccine 43 19596411
1996 Dimer/monomer equilibrium and domain separations of Escherichia coli ribosomal protein L7/L12. Biochemistry 43 8988004
1987 The structure and dynamics of ribosomal protein L12. Biochimie 43 3126823
2007 Structure of the base of the L7/L12 stalk of the Haloarcula marismortui large ribosomal subunit: analysis of L11 movements. Journal of molecular biology 42 17599351
2001 Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4. The EMBO journal 42 11500370
2015 PLGA (85:15) nanoparticle based delivery of rL7/L12 ribosomal protein in mice protects against Brucella abortus 544 infection: A promising alternate to traditional adjuvants. Molecular immunology 41 26442664
2008 CSIG inhibits PTEN translation in replicative senescence. Molecular and cellular biology 41 18678645
1996 Rotational and conformational dynamics of Escherichia coli ribosomal protein L7/L12. Biochemistry 41 8988003
1985 Preparation and characterization of two monoclonal antibodies against different epitopes in Escherichia coli ribosomal protein L7/L12. The Journal of biological chemistry 40 2581957
2017 Ribosomal protein L7/L12 is required for GTPase translation factors EF-G, RF3, and IF2 to bind in their GTP state to 70S ribosomes. The FEBS journal 39 28342293
2010 Increased exposure to bacterial antigen RpL7/L12 in early stage colorectal cancer patients. Cancer 39 20564125
1989 The flexible region of protein L12 from bacterial ribosomes studied by proton nuclear magnetic resonance. The Journal of biological chemistry 38 2647737
2001 Cryo-electron microscopic localization of protein L7/L12 within the Escherichia coli 70 S ribosome by difference mapping and Nanogold labeling. The Journal of biological chemistry 37 11278411
2000 Deletion of C-terminal residues of Escherichia coli ribosomal protein L10 causes the loss of binding of one L7/L12 dimer: ribosomes with one L7/L12 dimer are active. Biochemistry 36 10747797
1993 Structural and functional domains of Escherichia coli ribosomal protein L7/L12. The hinge region is required for activity. The Journal of biological chemistry 36 8419370
1996 Topology of the secondary structure elements of ribosomal protein L7/L12 from E. coli in solution. FEBS letters 34 8603708
2014 Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection. Vaccine 33 24598723
1989 Organization of genes encoding the L11, L1, L10, and L12 equivalent ribosomal proteins in eubacteria, archaebacteria, and eucaryotes. Canadian journal of microbiology 33 2497939
2014 Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection. Virology journal 32 24716528
2007 A combined DNA vaccine encoding BCSP31, SOD, and L7/L12 confers high protection against Brucella abortus 2308 by inducing specific CTL responses. DNA and cell biology 32 17570767
1999 Modulation of the gating of CIC-1 by S-(-) 2-(4-chlorophenoxy) propionic acid. British journal of pharmacology 32 10217531
1995 The hinge region of Escherichia coli ribosomal protein L7/L12 is required for factor binding and GTP hydrolysis. Biochimie 32 8834773
1983 Ribosomal protein L7/L12 cross-links to proteins in separate regions of the 50 S ribosomal subunit of Escherichia coli. The Journal of biological chemistry 32 6358224
1981 Chemistry and biology of E. coli ribosomal protein L12. Molecular and cellular biochemistry 32 7017380
2013 Evolution of the protein stoichiometry in the L12 stalk of bacterial and organellar ribosomes. Nature communications 31 23340427
1996 A delayed-early response nuclear gene encoding MRPL12, the mitochondrial homologue to the bacterial translational regulator L7/L12 protein. The Journal of biological chemistry 31 8626705
1994 Brucella ribosomal protein L7/L12 is a major component in the antigenicity of brucellin INRA for delayed-type hypersensitivity in brucella-sensitized guinea pigs. Infection and immunity 31 7960115
1981 Ribosomal protein modification in Escherichia coli. III. Studies of mutants lacking an acetylase activity specific for protein L12. Molecular & general genetics : MGG 31 7038378
2014 The antituberculosis antibiotic capreomycin inhibits protein synthesis by disrupting interaction between ribosomal proteins L12 and L10. Antimicrobial agents and chemotherapy 30 24449778
2023 Mitochondrial ribosomal protein L12 potentiates hepatocellular carcinoma by regulating mitochondrial biogenesis and metabolic reprogramming. Metabolism: clinical and experimental 29 38104924
2016 A residue substitution in the plastid ribosomal protein L12/AL1 produces defective plastid ribosome and causes early seedling lethality in rice. Plant molecular biology 29 26873698
1994 Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo. Journal of molecular biology 29 7932718
1990 Occurrence in the archaebacterium Sulfolobus solfataricus of a ribosomal protein complex corresponding to Escherichia coli (L7/L12)4.L10 and eukaryotic (P1)2/(P2)2.P0. The Journal of biological chemistry 29 2121730
2016 Feature Selection and Cancer Classification via Sparse Logistic Regression with the Hybrid L1/2 +2 Regularization. PloS one 28 27136190
2007 Escheriosome-mediated delivery of recombinant ribosomal L7/L12 protein confers protection against murine brucellosis. Vaccine 28 17931756
1979 Conformation of Escherichia coli ribosomal protein L7/L12 in solution: hydrodynamic, spectroscopic, and conformation prediction studies. Biochemistry 28 373797
1978 Guanosinetriphosphatase activity dependent on elongation factor Tu and ribosomal protein L7/L12. Proceedings of the National Academy of Sciences of the United States of America 28 210452
2022 RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression. Cell death & disease 27 35013134
1994 Molecular phylogenies based on ribosomal protein L11, L1, L10, and L12 sequences. Journal of molecular evolution 26 8007008
1989 Ribosomal protein L7/L12 has a helix-turn-helix motif similar to that found in DNA-binding regulatory proteins. Nucleic acids research 26 2660100
2012 Nucleolar protein CSIG is required for p33ING1 function in UV-induced apoptosis. Cell death & disease 25 22419112
2007 Functional interactions between the G' subdomain of bacterial translation factor EF-G and ribosomal protein L7/L12. The Journal of biological chemistry 25 17932030
1982 Fluorescence studies on the location of L7/L12 relative to L10 in the 50S ribosome of Escherichia coli. Biochemistry 25 7049235
2015 Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice. Molecular immunology 24 25723468
2008 Specific interactions of the L10(L12)4 ribosomal protein complex with mRNA, rRNA, and L11. Biochemistry 23 18247578
1990 Autoantibodies specific for the 20-KDal ribosomal large subunit protein L12. Biochemical and biophysical research communications 23 2241950
2020 Balancing Ligand Flexibility versus Rigidity for the Stepwise Self-Assembly of M12 L24 via M6 L12 Metal-Organic Cages. Chemistry (Weinheim an der Bergstrasse, Germany) 22 32378754
1998 A single-headed dimer of Escherichia coli ribosomal protein L7/L12 supports protein synthesis. Proceedings of the National Academy of Sciences of the United States of America 22 9539716
1991 Overexpression of L7/L12 protein with mutations in its flexible region. Biochimie 22 1799631
1981 The number of copies of ribosome-bound proteins L7 and L12 required for protein synthesis activity. The Journal of biological chemistry 22 6108955
1981 Structural analysis of ribosomal protein L7/L12 by the heterobifunctional cross-linker 4-(6-formyl-3-azidophenoxy)butyrimidate. Biochemistry 22 7011370
1976 DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro. Proceedings of the National Academy of Sciences of the United States of America 22 787986
1976 Accumulation of free ribosomal proteins S1, L7, and L12 in Escherichia coli. European journal of biochemistry 22 791647
2020 Comparison of Immune Effects Between Brucella Recombinant Omp10-Omp28-L7/L12 Proteins Expressed in Eukaryotic and Prokaryotic Systems. Frontiers in veterinary science 21 33195494
2015 CSIG promotes hepatocellular carcinoma proliferation by activating c-MYC expression. Oncotarget 21 25749381
1992 The C-terminal domain of Escherichia coli ribosomal protein L7/L12 can occupy a location near the factor-binding domain of the 50S subunit as shown by cross-linking with N-[4-(p-azidosalicylamido)butyl]-3-(2'-pyridyldithio)propionamide. Biochemistry 21 1390735
2015 Recombinant L7/L12 protein entrapping PLGA (poly lactide-co-glycolide) micro particles protect BALB/c mice against the virulent B. abortus 544 infection. Vaccine 20 25930114
2016 Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress. Scientific reports 19 27811966
1990 Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12. Journal of bacteriology 19 2403546
2012 Mechanism and rates of exchange of L7/L12 between ribosomes and the effects of binding EF-G. ACS chemical biology 18 22489843
2005 Immune responses of mice to vaccinia virus recombinants expressing either Listeria monocytogenes partial listeriolysin or Brucella abortus ribosomal L7/L12 protein. Veterinary microbiology 18 15941627
1998 A novel mutation in the L12 domain of keratin 5 in the Köbner variant of epidermolysis bullosa simplex. The Journal of investigative dermatology 18 9740251
1993 A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. The Journal of biological chemistry 18 7505271