CCDC97

CCDC97 is a poorly characterized protein implicated in pre-mRNA splicing through reported association with the SF3B splicing factor complex (PMID:41810918). Loss-of-function studies in hepatocellular carcinoma cells show that CCDC97 knockdown suppresses migration, invasion, and proliferation, indicating it is required for these malignant behaviors (PMID:39799421). Beyond these observations, no mechanistic detail linking the splicing association to the cellular phenotypes has been characterized in the available corpus.

1. 2025 Low

   To establish whether CCDC97 has any functional role in cancer cells, loss-of-function assays tested its requirement for malignant behaviors, showing it is needed for migration, invasion, and proliferation in HCC.

   Evidence siRNA/shRNA knockdown in HCC cell lines with migration, invasion, and proliferation assays

   PMID:39799421

   Open questions at the time

   • Single-lab in vitro phenotypic readouts without molecular mechanism or pathway placement
   • No in vivo validation of the tumor phenotype
   • No rescue experiment to confirm specificity
2. 2026 Low

   To place CCDC97 in a molecular pathway, it was reported to bind the SF3B splicing factor complex, implicating it in pre-mRNA splicing.

   Evidence Stated as a background interaction in a pan-cancer bioinformatics analysis with no primary experimental validation described

   PMID:41810918

   Open questions at the time

   • Interaction asserted as background fact without primary experimental validation in the source
   • No direct biochemical demonstration of SF3B binding
   • No link established between the splicing role and the HCC phenotype

• The molecular mechanism connecting CCDC97's reported splicing function to its requirement for cancer cell migration, invasion, and proliferation is unresolved.
• No splicing substrates or affected transcripts identified
• No structural or biochemical characterization of CCDC97
• No subcellular localization established in the corpus