Affinage

RSF1

Remodeling and spacing factor 1 · UniProt Q96T23

Length
1441 aa
Mass
163.8 kDa
Annotated
2026-06-10
70 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RSF1 (HBXAP) is the substrate-targeting subunit of the ISWI-family RSF chromatin remodeling complex, coupling nucleosome remodeling to DNA damage repair, mitotic chromosome segregation, and p53-dependent transcription (PMID:18519663, PMID:20923775, PMID:30242288). It binds the ATPase SNF2H/SMARCA5 through its Rsf-D4 region, an interaction that stabilizes RSF1 protein, redistributes SNF2H to the nucleus, and is obligatory for RSF complex remodeling activity (PMID:18519663, PMID:29385673). At DNA double-strand breaks RSF1 accumulates in an ATM-dependent manner via phosphorylated SQ motifs, physically engages ATM, and deposits the centromere proteins CENP-S/CENP-X to drive both NHEJ (through XRCC4 assembly) and homology-directed repair, while also recruiting resection factors RPA32 and Rad51 (PMID:24351651, PMID:23974106, PMID:24800743, PMID:40450933). RSF1 recruits HDAC1 to both centromeres and break sites, where deacetylation of histone H2A(X)-K118 licenses H2A-K119 ubiquitination, γH2AX propagation, and MDC1 recruitment, and at centromeres enables H2A-T120 phosphorylation and Sgo1 loading for accurate chromatid cohesion (PMID:30242288, PMID:34850117). In mitosis, CDK1-mediated phosphorylation of RSF1 at Ser1375 recruits PLK1 to kinetochores, and the RSF1–PLK1 axis spatially regulates Aurora B activation (via Thr236) for error correction and faithful chromosome segregation (PMID:26259146, PMID:34635673). RSF1 reads H2AK119ub through its UAB domain, linking it to Polycomb-marked chromatin (PMID:37529237). RSF1 also scaffolds p53/p300 and the FACT complex at target loci such as CDKN1A to support p53-dependent transcription following DNA damage (PMID:30348983, PMID:39579530). When overexpressed, RSF1 drives genomic instability, anchorage-independent growth, and chemoresistance, and cooperates with cyclin E1 to activate CDK2 in TP53-mutant cells (PMID:16172393, PMID:19190325, PMID:20923775, PMID:23378270).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 2002 Medium

    Established RSF1/HBXAP as a chromatin-associated transcriptional regulator able to coactivate viral and NF-κB transcription and to repress transcription via its PHD finger, framing it as a chromatin-acting protein before its remodeling role was known.

    Evidence Co-IP and in vitro binding with HBV pX plus GAL4 fusion reporter and deletion mutagenesis in cell lines

    PMID:11788598 PMID:11944984

    Open questions at the time
    • Did not identify the remodeling complex or ATPase partner
    • Mechanism of transcriptional effect through chromatin not defined
  2. 2004 Medium

    Localized RSF1 to the nuclear matrix and mapped distinct domains (coiled-coil targeting sequence and PHD finger) required for NF-κB repression, refining its transcriptional regulatory architecture.

    Evidence Co-IP, immunofluorescence colocalization, reporter assays with deletion mutagenesis

    PMID:15242768

    Open questions at the time
    • Direct vs. indirect NF-κB regulation not resolved
    • Relationship to remodeling activity unknown
  3. 2005 Medium

    Demonstrated that RSF1 functions as an oncogene whose amplification drives proliferation and transformation, motivating mechanistic dissection of its growth-promoting activity.

    Evidence Overexpression/knockdown with colony formation and anchorage-independent growth assays

    PMID:16172393

    Open questions at the time
    • Molecular mechanism of growth promotion not defined
    • Link to chromatin remodeling not established
  4. 2008 High

    Identified the core RSF complex by showing RSF1 binds SNF2H/SMARCA5, stabilizes it, and drives its nuclear localization, defining RSF1 as the targeting subunit of an ISWI remodeler.

    Evidence Co-IP, in vitro competition assay with domain mapping, immunofluorescence colocalization

    PMID:18519663

    Open questions at the time
    • Genomic targets of the complex not mapped
    • Remodeling biochemistry not reconstituted in this study
  5. 2009 Medium

    Linked RSF complex integrity to chemoresistance, showing the RSF1–SNF2H interaction is required for paclitaxel resistance and is therefore a functional, not incidental, partnership.

    Evidence siRNA, overexpression, complex disruption with drug resistance assays in ovarian cancer cells

    PMID:19190325

    Open questions at the time
    • Downstream effectors of resistance not identified
    • Single cancer context
  6. 2010 High

    Connected RSF1 overexpression to genomic instability, showing that excess RSF complex activity induces strand breaks and activates the ATM-CHK2-p53 DDR, explaining its oncogenic instability phenotype.

    Evidence Ectopic expression, comet assay, γH2AX IF, pathway analysis with TP53 KO and ATM inhibition

    PMID:20923775

    Open questions at the time
    • Direct mechanism producing breaks not defined
    • RSF1's role in normal (non-overexpressed) repair untested here
  7. 2013 High

    Defined RSF1 as a direct DSB repair factor: it accumulates at breaks via ATM, interacts with ATM, deposits CENP-S/CENP-X to promote NHEJ through XRCC4, and recruits RPA32/Rad51 for HR.

    Evidence Laser microirradiation, live-cell imaging, siRNA, ATM inhibition, recruitment and epistasis assays

    PMID:23974106 PMID:24351651

    Open questions at the time
    • How RSF1 selects between NHEJ and HR not resolved
    • Chromatin remodeling step at breaks not directly visualized
  8. 2013 High

    Identified cyclin E1 as an RSF1 partner that, in TP53-mutant cells, activates CDK2 and drives tumorigenesis, providing a distinct oncogenic mechanism beyond chromatin remodeling.

    Evidence Co-IP/mass spectrometry, domain mapping, CDK2 kinase assay, xenograft

    PMID:23378270

    Open questions at the time
    • Whether cyclin E1 binding depends on RSF complex unknown
    • Relationship to genomic instability phenotype not integrated
  9. 2013 Medium

    Showed RSF1 bridges the HBx viral protein to the mitotic checkpoint protein hBubR1 at kinetochores, linking RSF1 to chromosome segregation control.

    Evidence Co-IP, siRNA, deletion mutant analysis, kinetochore IF

    PMID:23536579

    Open questions at the time
    • Endogenous (non-viral) function at the checkpoint not established here
    • Structural basis of bridging unknown
  10. 2014 High

    Refined the DSB model by establishing that RSF1–ATM interaction drives CENP-S/X loading that regulates FANCD2/FANCI monoubiquitination, with RSF1 needed for repair but not checkpoint signaling.

    Evidence Reciprocal Co-IP, siRNA, microirradiation recruitment assays, HR/NHEJ reporter epistasis

    PMID:24800743

    Open questions at the time
    • How CENP-S/X feeds into the Fanconi pathway mechanistically unclear
    • Distinct repair vs. checkpoint separation of function not fully resolved
  11. 2015 High

    Established RSF1 as a kinetochore platform for PLK1, showing CDK1 phosphorylation at Ser1375 recruits PLK1 and a PLK1 feedback phosphorylation at Ser1359 stabilizes it, governing chromosome alignment.

    Evidence In vitro CDK1/PLK1 kinase assays, phosphomutant rescue, siRNA, live-cell imaging

    PMID:26259146

    Open questions at the time
    • Connection to RSF complex remodeling at kinetochores not defined
    • Spatial regulation of the axis not yet established at this stage
  12. 2018 High

    Demonstrated RSF1 functions as a histone-modification regulator at centromeres by recruiting HDAC1 to deacetylate H2A-K118, enabling H2A-T120 phosphorylation and Sgo1 loading for accurate segregation.

    Evidence RSF1 knockout, Co-IP, ChIP, IF, HDAC1 centromeric tethering rescue

    PMID:30242288

    Open questions at the time
    • How RSF1 spatially targets HDAC1 to centromeres unclear
    • Crosstalk with the PLK1/Aurora B axis not yet integrated
  13. 2018 High

    Extended RSF1's transcriptional role to p53 signaling, showing it is required for p53/p300 recruitment and H3 acetylation at p53 target promoters in vivo following DNA damage.

    Evidence Neural-specific Rsf1 knockout mouse, ChIP, transcriptome analysis

    PMID:30348983

    Open questions at the time
    • Direct vs. indirect scaffolding of p53/p300 not dissected here
    • Specific target gene mechanisms not detailed
  14. 2018 Medium

    Revealed post-translational control of RSF1 abundance during damage: SNF2H stabilizes RSF1 while ATM phosphorylation at SQ sites destabilizes it, showing temporal level control is required for repair.

    Evidence Western blot under damage conditions, siRNA, phosphomutant overexpression, ATM manipulation, repair assay

    PMID:29385673

    Open questions at the time
    • Degradation machinery acting on phospho-RSF1 not identified
    • Quantitative kinetics of level changes not defined
  15. 2021 High

    Unified the RSF1-HDAC1 histone crosstalk at DSBs, showing H2A(X)-K118 deacetylation is required for H2A-K119 ubiquitination, γH2AX spreading, MDC1 recruitment, and damage-site transcriptional repression.

    Evidence RSF1 depletion, phospho/acetyl histone mutants, ChIP, IF, γH2AX analysis

    PMID:34850117

    Open questions at the time
    • Identity of the K119 ubiquitin ligase recruited downstream not defined here
    • Generality across chromatin contexts untested
  16. 2021 High

    Defined the spatial logic of the RSF1-PLK1 axis in mitosis, showing it activates Aurora B (Thr236) at centromeres for error correction and relocates to kinetochores upon attachment, controlled by H3-S28 phosphorylation.

    Evidence In vitro kinase assays, structural modeling, phosphomutants, IF, siRNA, live-cell imaging

    PMID:34635673

    Open questions at the time
    • Trigger coupling attachment status to RSF1 movement not fully mechanistic
    • How remodeling activity contributes to relocation unknown
  17. 2021 Medium

    Linked RSF1 to a pro-angiogenic transcriptional program in cancer, showing it cotransactivates the IL1B promoter with SNF2H and CEBP/β to drive IL-1β-mediated angiogenesis.

    Evidence siRNA, RT-PCR, reporter assay, IL-1β ELISA, angiogenesis assay, xenograft

    PMID:33496909

    Open questions at the time
    • Direct promoter occupancy mechanism not fully mapped
    • Single tumor type (myxofibrosarcoma)
  18. 2023 Medium

    Identified the molecular basis for RSF1 chromatin targeting, showing its UAB domain reads H2AK119ub and is required for recruitment to Polycomb-marked target genes in development.

    Evidence Xenopus morpholino knockdown, deletion-mutant rescue, ectopic H2AK119ub deposition, immunoprecipitation

    PMID:37529237

    Open questions at the time
    • Structural details of UAB-ubiquitin recognition not resolved
    • Generality to human RSF1 targeting not directly tested
  19. 2024 Medium

    Detailed RSF1's coactivator scaffolding at CDKN1A, showing it promotes p300 enhancer binding and FACT (SSRP1/SPT16) promoter recruitment to support p53-dependent p21 transcription.

    Evidence Co-IP (RSF1-p300), ChIP in RSF1 KO cells, Western blot

    PMID:39579530

    Open questions at the time
    • Order of p300 and FACT recruitment not resolved
    • Whether SNF2H remodeling is required not tested
  20. 2025 Medium

    Showed RSF1 itself is a regulated acetylation substrate, with KAT8 acetylating RSF1 at K1050 in a pathway linked to heterochromatin loss and skin aging, governed upstream by FTO/m6A control of KAT8.

    Evidence RNA-seq, MeRIP-seq, acetylation proteomics, siRNA/inhibitor, m6A assays, Western blot

    PMID:40636284

    Open questions at the time
    • Functional consequence of K1050 acetylation on RSF activity not dissected
    • Direct causality between RSF1 acetylation and heterochromatin loss not established
  21. 2025 Medium

    Quantified the kinetics of RSF1/CENP-S/CENP-X at breaks, placing their simultaneous arrival (~100 s) downstream of ATM and RNF8-RNF168 across cell cycle phases, temporally anchoring RSF1 to early DSB nucleosome remodeling.

    Evidence Live-cell microirradiation, quantitative time-course imaging, cell cycle analysis

    PMID:40450933

    Open questions at the time
    • Mechanism of co-recruitment as a unit not defined
    • Removal kinetics determinants unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RSF1 integrates its remodeling, histone-reading, and kinase-scaffolding functions into a single regulated platform—and how acetylation/phosphorylation of RSF1 itself toggles between these roles—remains unresolved.
  • No integrated structural model of full-length RSF1 with its modifications
  • Switch between repair, mitotic, and transcriptional roles uncoordinated in current data
  • Reconstitution of UAB-directed targeting with SNF2H remodeling missing

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0140110 transcription regulator activity 3 GO:0042393 histone binding 2 GO:0140097 catalytic activity, acting on DNA 2 GO:0140657 ATP-dependent activity 1
Localization
GO:0000228 nuclear chromosome 3 GO:0005694 chromosome 3 GO:0005634 nucleus 2 GO:0005654 nucleoplasm 2
Pathway
R-HSA-73894 DNA Repair 4 R-HSA-1640170 Cell Cycle 3 R-HSA-4839726 Chromatin organization 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-8953897 Cellular responses to stimuli 2
Partners
ATMBUB1BCCNE1CEBPBEP300HDAC1PLK1SMARCA5
Complex memberships
RSF complex (RSF1-SNF2H/SMARCA5)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 HBXAP (RSF1) physically interacts with hepatitis B virus X protein (pX) in vitro and in vivo via the HBXAP region containing the PHD finger domain. This interaction coactivates HBV transcription and NF-κB-mediated transcription. Co-immunoprecipitation, in vitro binding assay, transcription reporter assay The Journal of biological chemistry Medium 11788598
2002 HBXAP (RSF1) possesses intrinsic transcriptional repression activity when recruited to DNA via GAL4; the PHD finger domain alone is sufficient for this repression activity. GAL4 fusion transcription reporter assay, deletion mutagenesis Genomics Medium 11944984
2004 HBXAP (RSF1) represses NF-κB-mediated gene activation in a dose-dependent manner; HBXAP and NF-κB colocalize at the nuclear matrix with direct physical interaction. A nuclear matrix targeting sequence (aa 688–722, coiled-coil region) and the PHD finger domain are both required for this repression. Co-immunoprecipitation, immunofluorescence colocalization, reporter gene assay, deletion mutagenesis Experimental cell research Medium 15242768
2005 RSF1 (HBXAP) overexpression stimulates cell proliferation and confers serum-independent and anchorage-independent growth in non-neoplastic cells; RSF1 knockdown inhibits growth in cells with RSF1 amplification. Overexpression/knockdown in cell lines, colony formation and growth assays Proceedings of the National Academy of Sciences of the United States of America Medium 16172393
2008 RSF1 interacts with hSNF2H (SMARCA5) to form a chromatin remodeling complex (RSF complex). Ectopic RSF1 expression upregulates hSNF2H protein levels and translocates hSNF2H to the nucleus where it colocalizes with RSF1. The hSNF2H binding site maps to the Rsf-D4 fragment of RSF1 by coimmunoprecipitation and in vitro competition assay. Co-immunoprecipitation, in vitro competition assay, immunofluorescence colocalization, deletion mutagenesis Cancer research High 18519663
2009 RSF1 promotes paclitaxel resistance in ovarian cancer cells; this resistance requires formation of the RSF1/hSNF2H complex, as disruption of hSNF2H or the RSF1-hSNF2H interaction restores paclitaxel sensitivity. siRNA knockdown, ectopic overexpression, drug resistance assays, disruption of complex Cancer research Medium 19190325
2010 RSF1 overexpression induces DNA strand breaks, nuclear γH2AX foci, and activates the ATM-CHK2-p53-p21 DNA damage response pathway. Formation of a functional RSF complex with SNF2H is required for RSF1 to trigger this DDR. Chronic RSF1 expression leads to chromosomal aberrations, c-myc amplification, and CDKN2A/B deletion. Ectopic expression, deletion mutagenesis, gene knockdown, comet assay, γH2AX immunofluorescence, chromosomal analysis, TP53 knockout The Journal of biological chemistry High 20923775
2013 RSF1 interacts with cyclin E1 as identified by co-immunoprecipitation followed by nanoelectrospray mass spectrometry. Ectopic co-expression of RSF1 and cyclin E1 in TP53-mutant cells activates CDK2 and promotes cellular proliferation and tumor formation; the cyclin E1-binding site maps to the first 441 amino acids of RSF1. Co-immunoprecipitation, nanoelectrospray mass spectrometry, ectopic expression, domain mapping, CDK2 kinase assay, xenograft The Journal of pathology High 23378270
2013 RSF1 accumulates at DNA double-strand break (DSB) sites in an ATM-dependent manner; putative ATM phosphorylation sites (pSQ motifs) on RSF1 are required for its DSB accumulation. RSF1 depletion attenuates DNA damage checkpoint signaling and reduces cell survival after damage. RSF1 promotes homologous recombination repair by recruiting resection factors RPA32 and Rad51. Laser microirradiation, live-cell imaging, immunofluorescence, siRNA depletion, ATM inhibition, RPA32/Rad51 recruitment assay Cell cycle (Georgetown, Tex.) Medium 24351651
2013 RSF1 deposits centromere proteins CENP-S and CENP-X at DNA DSB sites to promote NHEJ. CENP-S and CENP-X in turn facilitate assembly of NHEJ factor XRCC4 at damaged chromatin. RSF1 also promotes HR repair independently of CENP-S/CENP-X. SMARCA5 regulates RNF168-dependent ubiquitin response (BRCA1 recruitment) but RSF1 is dispensable for this. siRNA knockdown, laser microirradiation, immunofluorescence recruitment assay, IR survival assay, epistasis analysis Cell cycle (Georgetown, Tex.) High 23974106
2013 HBxAPα/RSF1 mediates interaction between HBx viral protein and hBubR1 at the chromatin fraction during mitosis. RSF1 depletion abolishes the HBx-hBubR1 interaction and impairs HBx targeting to kinetochores. HBx binding to hBubR1 (stabilized by RSF1) reduces hBubR1-Cdc20 binding and increases mitotic aberrations. Co-immunoprecipitation, siRNA knockdown, deletion mutant analysis, kinetochore immunofluorescence Carcinogenesis Medium 23536579
2014 RSF1 physically interacts with ATM in a manner dependent on DSBs and ATM kinase activity. RSF1 is required for ATM-dependent recruitment of CENP-S/MHF1 and CENP-X/MHF2 to DSBs. These proteins in turn regulate mono-ubiquitination of Fanconi Anaemia proteins FANCD2 and FANCI. RSF1 is required for efficient DSB repair via both end-joining and homology-directed repair but is not required for checkpoint signaling. Co-immunoprecipitation, siRNA knockdown, laser microirradiation, immunofluorescence recruitment assay, epistasis with HR and NHEJ reporters PLoS biology High 24800743
2015 RSF1 localizes at mitotic kinetochores and directly binds PLK1. CDK1 phosphorylates RSF1 at Ser1375, which is necessary for PLK1 recruitment to kinetochores. Subsequently PLK1 phosphorylates RSF1 at Ser1359, stabilizing PLK1 deposition. RSF1 depletion causes chromosome misalignment phenotype mimicking PLK1 knockdown; rescued by RSF1 S1375D or S1359D but not S1375A phospho-mutants. Co-immunoprecipitation, immunofluorescence, siRNA knockdown, phosphomutant rescue, in vitro kinase assay (CDK1 and PLK1), live-cell imaging Nature communications High 26259146
2018 RSF1 is required for p53-dependent gene transcription in response to DNA strand breaks. RSF1 deficiency reduces binding of the p53/p300 complex and subsequent H3 acetylation at p53 target gene promoters, causing reduced expression of p53 downstream genes. Neural-specific Rsf1 knockout mouse, chromatin immunoprecipitation, transcriptome analysis, Western blot, immunofluorescence Cell death & disease High 30348983
2018 RSF1 protein levels are transiently upregulated in response to DNA damage at the post-translational level (without changes in mRNA). SNF2H binding partner stabilizes RSF1 protein; ATM-mediated phosphorylation of RSF1 at three SQ sites (RSF1-3SA mutant) destabilizes the protein. Proper temporal regulation of RSF1 levels by SNF2H and ATM is essential for efficient DSB repair. Western blot under various DNA damage conditions, siRNA knockdown, phosphomutant overexpression, ATM kinase manipulation, DSB repair assay Molecules and cells Medium 29385673
2018 RSF1 recruits HDAC1 to centromeres, where HDAC1 counteracts TIP60-mediated acetylation of histone H2A at K118. This deacetylation enables accumulation of H2A phosphorylation at T120 (by Bub1) and Sgo1 deposition at centromeres, which is required for faithful chromosome segregation. Centromeric HDAC1 tethering prevents premature chromatid separation in RSF1 knockout cells. Co-immunoprecipitation, RSF1 knockout, chromatin immunoprecipitation, immunofluorescence, rescue experiments with HDAC1 centromeric tethering Nature communications High 30242288
2021 Under DNA damage, RSF1 recruits HDAC1 to DSB sites. The RSF1-HDAC1 complex deacetylates H2A(X) at K118, which is required for H2A ubiquitination at K119 (H2A-K119ub) and subsequent transcriptional repression at DNA lesions. Deacetylation of H2AX-K118 also enables γH2AX propagation and MDC1 recruitment, promoting DNA repair. H2A-K118Q acetylation mimetic suppresses H2A-K119ub and impairs repair. RSF1 depletion, phospho/acetyl mutant analysis, chromatin immunoprecipitation, immunofluorescence, γH2AX analysis Nucleic acids research High 34850117
2021 RSF1 PLK1-axis at centromeres creates an activating phosphorylation on Aurora B at Thr236 (in the activation loop), which is indispensable for Aurora B activation and subsequent microtubule destabilization in error correction. Under full microtubule-kinetochore attachment, RSF1-PLK1 moves to kinetochores, halts Aurora B activation, and phosphorylates BubR1. Aurora B-mediated phosphorylation of centromeric histone H3 at Ser28 triggers the spatial RSF1-PLK1 movement from centromeres to kinetochores. In vitro kinase assay, structural modeling, phosphomutant analysis, immunofluorescence, siRNA depletion, live-cell imaging Nature communications High 34635673
2021 RSF1 requires hSNF2H and CEBP/β to cotransactivate the IL1B promoter, increasing IL-1β mRNA, secretion, and downstream angiogenic capacity in myxofibrosarcoma cells. RSF1-driven angiogenesis is counteracted by IL1B knockdown and IL-1β-neutralizing antibodies. siRNA knockdown, RT-PCR profiling, chromatin reporter assay, IL-1β ELISA, angiogenesis assay, in vivo xenograft Angiogenesis Medium 33496909
2023 RSF1 acts as a reader of histone H2A lysine 119 ubiquitination (H2AK119ub). The UAB domain of RSF1 is required to recognize H2AK119ub nucleosomes; deletion of the UAB domain abolishes the ability of RSF1 to rescue developmental defects in Xenopus rsf1 morphants. Ectopic deposition of H2AK119ub recruits RSF1 to the Smad2 target gene gsc. Xenopus laevis morpholino knockdown, rescue with deletion mutants, in vivo H2AK119ub deposition using ring1a-smad2 fusion, immunoprecipitation Frontiers in cell and developmental biology Medium 37529237
2024 RSF1 promotes p53-dependent p21 (CDKN1A) gene transcription by facilitating accumulation of p300 acetyltransferase at the enhancer and FACT complex (SSRP1, SPT16) at the promoter of CDKN1A. RSF1 depletion reduces acetylated-K382 p53 levels and decreases p300 and TBP binding along with reduced H3K27ac and H3K4me1 at the CDKN1A locus. Co-immunoprecipitation (RSF1 with p300), chromatin immunoprecipitation, RSF1 knockout cells, Western blot Biochemical and biophysical research communications Medium 39579530
2025 KAT8 acetyltransferase acetylates RSF1 at K1050, and this acetylation is associated with heterochromatin loss and cellular senescence during skin aging. FTO depletion reduces KAT8 mRNA stability via m6A-YTHDF2 pathway, leading to reduced KAT8 and downstream RSF1 K1050 acetylation. RNA-seq, MeRIP-seq, acetylation proteomics, siRNA/inhibitor knockdown, m6A modification assays, Western blot MedComm Medium 40636284
2025 RSF1 and CENP-S/CENPX are recruited to DSBs with a half-time of ~100 s and removed with a half-time of ~2000 s. Recruitment occurs in G1, S, and G2 phases, with stronger/delayed enrichment in G2. RSF1/CENP-S/CENPX arrive simultaneously after ATM activation and RNF8-RNF168 activity, temporally placing them at the time of nucleosome remodeling during early DSB response. Live-cell microirradiation, quantitative fluorescence time-course imaging, cell cycle phase analysis DNA repair Medium 40450933

Source papers

Stage 0 corpus · 70 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Amplification of a chromatin remodeling gene, Rsf-1/HBXAP, in ovarian carcinoma. Proceedings of the National Academy of Sciences of the United States of America 108 16172393
2010 Serous tubal intraepithelial carcinoma upregulates markers associated with high-grade serous carcinomas including Rsf-1 (HBXAP), cyclin E and fatty acid synthase. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 79 20228782
2018 LncRNA NEAT1/let-7a-5p axis regulates the cisplatin resistance in nasopharyngeal carcinoma by targeting Rsf-1 and modulating the Ras-MAPK pathway. Cancer biology & therapy 76 29565706
2009 Functional analysis of 11q13.5 amplicon identifies Rsf-1 (HBXAP) as a gene involved in paclitaxel resistance in ovarian cancer. Cancer research 60 19190325
2010 Rsf-1, a chromatin remodeling protein, induces DNA damage and promotes genomic instability. The Journal of biological chemistry 54 20923775
2013 Remodeling and spacing factor 1 (RSF1) deposits centromere proteins at DNA double-strand breaks to promote non-homologous end-joining. Cell cycle (Georgetown, Tex.) 53 23974106
2021 CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis. Biological research 52 33757583
1999 Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development. Genes & development 50 10090730
2016 Two asian jumbo phages, ϕRSL2 and ϕRSF1, infect Ralstonia solanacearum and show common features of ϕKZ-related phages. Virology 49 27081857
2008 The roles of human sucrose nonfermenting protein 2 homologue in the tumor-promoting functions of Rsf-1. Cancer research 48 18519663
2002 Hepatitis B virus pX interacts with HBXAP, a PHD finger protein to coactivate transcription. The Journal of biological chemistry 46 11788598
2018 RSF-1 overexpression determines cancer progression and drug resistance in cervical cancer. BioMedicine 42 29480799
2011 Overexpression of a chromatin remodeling factor, RSF-1/HBXAP, correlates with aggressive oral squamous cell carcinoma. The American journal of pathology 41 21514451
2014 The RSF1 histone-remodelling factor facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins. PLoS biology 40 24800743
2020 Circular RNA RSF1 promotes inflammatory and fibrotic phenotypes of irradiated hepatic stellate cell by modulating miR-146a-5p. Journal of cellular physiology 39 31960423
2017 Rsf-1 Influences the Sensitivity of Non-Small Cell Lung Cancer to Paclitaxel by Regulating NF-κB Pathway and Its Downstream Proteins. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 36 29258089
2013 ATM-dependent chromatin remodeler Rsf-1 facilitates DNA damage checkpoints and homologous recombination repair. Cell cycle (Georgetown, Tex.) 34 24351651
2002 HBXAP, a novel PHD-finger protein, possesses transcription repression activity. Genomics 34 11944984
2013 Rsf-1, a chromatin remodelling protein, interacts with cyclin E1 and promotes tumour development. The Journal of pathology 33 23378270
2006 Expression of Rsf-1, a chromatin-remodeling gene, in ovarian and breast carcinoma. Human pathology 31 16938522
2020 lncRNA-PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1/NF-κB Signaling Pathway. Cancer biotherapy & radiopharmaceuticals 29 33156701
2022 linc00958/miR-185-5p/RSF-1 modulates cisplatin resistance and angiogenesis through AKT1/GSK3β/VEGFA pathway in cervical cancer. Reproductive biology and endocrinology : RB&E 27 36056431
2020 Long Noncoding RNA SNHG6 Promotes Proliferation and Inhibits Apoptosis in Non-small Cell Lung Cancer Cells by Regulating miR-490-3p/RSF1 Axis. Cancer biotherapy & radiopharmaceuticals 27 32202941
2015 The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nature communications 27 26259146
2011 Associations of Rsf-1 overexpression with poor therapeutic response and worse survival in patients with nasopharyngeal carcinoma. Journal of clinical pathology 26 22081787
2018 The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nature communications 22 30242288
2018 Chromatin-remodeling factor, RSF1, controls p53-mediated transcription in apoptosis upon DNA strand breaks. Cell death & disease 22 30348983
1999 Recognition of exonic splicing enhancer sequences by the Drosophila splicing repressor RSF1. Nucleic acids research 22 10325428
2017 RSF1 regulates the proliferation and paclitaxel resistance via modulating NF-κB signaling pathway in nasopharyngeal carcinoma. Journal of Cancer 20 28261335
2012 Rsf-1 is overexpressed in non-small cell lung cancers and regulates cyclinD1 expression and ERK activity. Biochemical and biophysical research communications 20 22387541
2017 RSF1 functions as an oncogene in osteosarcoma and is regulated by XIST/miR-193a-3p axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 19 28843909
2013 HBxAPα/Rsf-1-mediated HBx-hBubR1 interactions regulate the mitotic spindle checkpoint and chromosome instability. Carcinogenesis 17 23536579
2011 Rsf-1 (HBXAP) expression is associated with advanced stage and lymph node metastasis in ovarian clear cell carcinoma. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 17 21131837
2011 Rsf-1/HBXAP overexpression is associated with disease-specific survival of patients with gallbladder carcinoma. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 17 21995635
2021 The chromatin remodeler RSF1 coordinates epigenetic marks for transcriptional repression and DSB repair. Nucleic acids research 16 34850117
2020 LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis. Cancer management and research 15 33244266
2023 Exosome-delivered circRNA circSYT15 contributes to cisplatin resistance in cervical cancer cells through the miR-503-5p/RSF1 axis. Cell cycle (Georgetown, Tex.) 14 37974391
2015 Overexpression of hSNF2H in glioma promotes cell proliferation, invasion, and chemoresistance through its interaction with Rsf-1. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 14 26666816
2011 DNA Damage Response is Prominent in Ovarian High-Grade Serous Carcinomas, Especially Those with Rsf-1 (HBXAP) Overexpression. Journal of oncology 14 22028712
2004 Functional interaction between nuclear matrix-associated HBXAP and NF-kappaB. Experimental cell research 14 15242768
2021 RSF1 in cancer: interactions and functions. Cancer cell international 13 34147108
2021 Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms. Nature communications 13 34635673
2020 HMGB1 regulates SNAI1 during NSCLC metastasis, both directly, through transcriptional activation, and indirectly, in a RSF1-IT2-dependent manner. Molecular oncology 13 32306523
2012 Rsf-1/HBXAP overexpression is independent of gene amplification and is associated with poor outcome in patients with urinary bladder urothelial carcinoma. Journal of clinical pathology 13 22685262
2020 RSF-1 siRNA Enhances Tumor Radiosensitivity in Cervical Cancer via Enhanced DNA Damage, Cell Cycle Redistribution, and Promotion of Apoptosis. OncoTargets and therapy 11 32308437
2014 Overexpression of Rsf-1 correlates with pathological type, p53 status and survival in primary breast cancer. International journal of clinical and experimental pathology 11 25337201
2018 Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage. Molecules and cells 10 29385673
2021 RSF1 requires CEBP/β and hSNF2H to promote IL-1β-mediated angiogenesis: the clinical and therapeutic relevance of RSF1 overexpression and amplification in myxofibrosarcomas. Angiogenesis 9 33496909
2019 Rsf‑1 regulates malignant melanoma cell viability and chemoresistance via NF‑κB/Bcl‑2 signaling. Molecular medicine reports 9 31485613
2013 RSF1 and not cyclin D1 gene amplification may predict lack of benefit from adjuvant tamoxifen in high-risk pre-menopausal women in the MA.12 randomized clinical trial. PloS one 8 24367492
2012 Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Experimental cell research 8 23103556
2021 Let-7a targets Rsf-1 to modulate radiotherapy response of non-small cell lung cancer cells through Ras-MAPK pathway. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 5 34565000
2010 Dendritic cells transduced with Rsf-1/HBXAP gene generate specific cytotoxic T lymphocytes against ovarian cancer in vitro. Biochemical and biophysical research communications 5 20226169
2008 Expression of the chromatin remodeling factor Rsf-1 is down-regulated in breast carcinoma effusions. Human pathology 5 18289639
2022 miR-129-5p Plays an Anticancer Role in Colon Cancer by Targeting RSF1. Cellular and molecular biology (Noisy-le-Grand, France) 4 35818253
2018 Effects of RSF-1 on proliferation and apoptosis of breast cancer cells. Oncology letters 4 30214561
2025 MicroRNA miR-193b-3p Regulates Esophageal Cancer Progression Through Targeting RSF1. Cells 3 40558555
2025 Depletion of Fat Mass and Obesity-Associated Protein (FTO) Drives Heterochromatin Loss via Lysine Acetyltransferase 8 (KAT8)-Mediated Remodeling and Spacing Factor 1 (RSF1) Acetylation in Skin Aging. MedComm 3 40636284
2023 Recognition of H2AK119ub plays an important role in RSF1-regulated early Xenopus development. Frontiers in cell and developmental biology 3 37529237
2022 Transcriptome Sequencing Reveal That Rno-Rsf1_0012 Participates in Levodopa-Induced Dyskinesia in Parkinson's Disease Rats via Binding to Rno-mir-298-5p. Brain sciences 2 36138942
2013 Quantification of serum HBXAP DNA in lung cancer patients by quantitative fluorescent polymerase chain reaction. Molecular biology reports 2 23456648
1991 The RSF1 gene regulates septum formation in Saccharomyces cerevisiae. Journal of bacteriology 2 2013573
2026 De novo heterozygous variants of the RSF1 gene are responsible for a syndromic neurodevelopmental disorder. European journal of human genetics : EJHG 1 41606215
2025 Dynamics of chromatin factors RSF1, CENPS and CENPX at DNA damage sites. DNA repair 1 40450933
2025 Chromatin Remodeler RSF1 as an Oncogenic Driver and Therapeutic Target in Esophageal Squamous Cell Carcinoma. Cells 1 40862741
2024 RSF1 orchestrates p53 transcriptional activity by coordinating p300 acetyltransferase and FACT complex. Biochemical and biophysical research communications 1 39579530
2017 Correction: The RSF1 Histone-Remodelling Factor Facilitates DNA Double Strand Break Repair by Recruiting Centromeric and Fanconi Anaemia Proteins. PLoS biology 1 28146553
2015 A novel protein, Rsf1/Pxd1, is critical for the single-strand annealing pathway of double-strand break repair in Schizosaccharomyces pombe. Molecular microbiology 1 25777942
2026 RSF-1/hSNF2H Promotes the Proliferation and Migration of Adenoid Cystic Cancer Cells. Bulletin of experimental biology and medicine 0 41949721
2025 Correction to "Depletion of Fat Mass and Obesity-Associated Protein (FTO) Drives Heterochromatin Loss via Lysine Acetyltransferase 8 (KAT8)-Mediated Remodeling and Spacing Factor 1 (RSF1) Acetylation in Skin Aging". MedComm 0 40979215

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