Affinage

RPS5

Small ribosomal subunit protein uS7 · UniProt P46782

Length
204 aa
Mass
22.9 kDa
Annotated
2026-06-10
100 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPS5 (uS7) is a conserved small ribosomal subunit protein that occupies the 40S/30S mRNA exit channel and platform region, where it organizes decoding-region rRNA and governs the accuracy of both start-codon selection and elongation (PMID:26134896, PMID:1508272). Its β-hairpin protrudes into the mRNA exit channel and is essential for efficient and accurate AUG recognition: substitutions in β-strand-1 reduce ternary-complex binding to the closed (PIN) conformation of reconstituted 43S·mRNA complexes, promote leaky scanning, and lower initiation fidelity (PMID:26134896). The interface between uS7 and eIF2α is remodeled during scanning, sequentially stabilizing the open then closed pre-initiation conformations; mutations that perturb the open-state contacts permit initiation at suboptimal UUG codons, whereas mutations at the closed-state interface confer hyperaccuracy and accelerated ternary-complex dissociation (PMID:28169832). The bacterial ortholog (E. coli S5) contributes to decoding fidelity through two structurally distinct domains—a C-terminal domain organizing decoding-region rRNA where accuracy-impairing (ram) mutations cluster, and an N-terminal domain contacting the spectinomycin-binding 16S rRNA helix—with the S4–S5 interface acting as the locus of the domain-closure mechanism for tRNA selection (PMID:1508272, PMID:9642068, PMID:25548247). Recruitment of initiation factors eIF2 and eIF3 and proper translation depend on the protein's N-terminal extension and N-terminal residues (PMID:17901157, PMID:19969550), and the C-terminus is required for 3′ processing of 18S rRNA precursors despite normal head-domain assembly (PMID:20419091). Beyond the ribosome, RPS5 directly binds the hepatitis C virus IRES (domains II and IV) via its β-hairpin to position viral RNA on the 40S subunit and drive internal initiation (PMID:11331271, PMID:25712089), and in hepatic stellate cells and cardiac fibroblasts RPS5 acts upstream of Akt and p38 signaling to suppress fibrogenic activation (PMID:24668691, PMID:32694761).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1992 High

    Establishing the domain architecture of S5 was needed to explain how a single ribosomal protein could affect both antibiotic resistance and decoding accuracy; the crystal structure resolved this by assigning the two phenotypes to two distinct domains.

    Evidence X-ray crystallography of E. coli S5 with mutation mapping

    PMID:1508272

    Open questions at the time
    • Domain assignments were inferred from mutation phenotypes rather than direct rRNA-bound structures
    • Did not define eukaryotic-specific features
  2. 1988 Medium

    To know what S5 actually contacts in the ribosome, chemical and cross-linking probing mapped specific 16S rRNA elements near the decoding center protected or contacted by S5.

    Evidence Chemical footprinting and photo-affinity cross-linking in reconstituted 30S subunits

    PMID:2437527 PMID:2459389

    Open questions at the time
    • Contacts mapped at nucleotide resolution but functional consequences not directly tested
    • Performed in bacterial system
  3. 1990 Medium

    Whether the bacterial fidelity function was conserved in eukaryotes was unknown; cloning yeast SUP44 and mapping its suppressor mutation to the conserved ram region demonstrated cross-kingdom conservation of translational fidelity control.

    Evidence Gene cloning, sequencing, and suppressor mutation mapping in yeast

    PMID:2247072

    Open questions at the time
    • Conservation inferred from sequence and genetics, not biochemistry
    • Mechanism of fidelity modulation not resolved
  4. 2006 Medium

    It was unclear whether fidelity was governed by a single region; the remote G28D mutation showed multiple distinct regions of S5 independently modulate frameshifting and read-through, dissociating these from initiation.

    Evidence Site-directed mutagenesis with frameshifting/read-through reporters and ribosome profiling in E. coli

    PMID:17053085

    Open questions at the time
    • Structural basis for the remote-site effect not defined
    • Did not address eukaryotic initiation
  5. 2008 Medium

    The relationship between N-terminal acetylation of S5 and ribosome function was unresolved; RimJ was shown to act as an assembly factor independent of its acetyltransferase activity, separating its enzymatic and structural roles.

    Evidence Suppressor screen, acetyltransferase-dead complementation, and pre-30S association assays in E. coli

    PMID:18466225 PMID:2828880

    Open questions at the time
    • Mechanism of assembly chaperone activity unresolved
    • No eukaryotic counterpart established
  6. 2014 Medium

    How accuracy-modulating mutations relate to subunit dynamics was unclear; systematic rpsE mutagenesis localized accuracy effects to the S4–S5 interface, supporting the domain-closure model of tRNA selection.

    Evidence Site-directed and random mutagenesis with fidelity reporters and antibiotic profiling in E. coli

    PMID:25548247

    Open questions at the time
    • Domain-closure model inferred from genetics, not direct dynamics
    • Bacterial system only
  7. 2015 High

    The precise structural element of S5 driving start-codon recognition was undefined; the β-hairpin in the mRNA exit channel was shown to be essential for accurate AUG selection by stabilizing the closed PIC conformation.

    Evidence Yeast genetics plus in vitro reconstitution of 43S·mRNA complexes with TC-binding assays

    PMID:26134896

    Open questions at the time
    • Did not resolve the eIF2α contact dynamics
    • Effect on eIF1 levels not mechanistically dissected
  8. 2017 High

    The dynamic role of the uS7–eIF2α contact during scanning was unknown; mutagenesis showed the interface is sequentially remodeled to stabilize open then closed PIC states, directly linking uS7 to fidelity through factor contacts.

    Evidence Yeast fidelity assays plus in vitro PIC reconstitution and cryo-EM-guided mutagenesis

    PMID:28169832

    Open questions at the time
    • Atomic structures of the transition states not provided
    • Generality across mRNA contexts not fully tested
  9. 2009 Medium

    The contribution of the S5 N-terminus to initiation factor recruitment was undefined; truncation analysis showed progressive loss of eIF2 and eIF3 recruitment with N-terminal deletion.

    Evidence Yeast truncation genetics with growth and factor-association assays

    PMID:19969550

    Open questions at the time
    • Direct binding interface not mapped
    • Distinguishing assembly vs. function defects incomplete
  10. 2007 Medium

    Whether species-specific N-terminal sequences tune ribosome behavior was unknown; replacing the fungal-specific N-terminal extension with human rpS5 altered elongation fidelity and IRES interaction.

    Evidence Yeast gene replacement with frameshifting, read-through, and CrPV IRES binding assays

    PMID:17901157

    Open questions at the time
    • Structural basis of extension-dependent effects unresolved
    • Human extension absent so direct ortholog comparison limited
  11. 2010 Medium

    Whether S5 has a role in ribosome maturation beyond decoding was unaddressed; C-terminal truncation showed it is required for 18S rRNA 3′ processing despite normal head assembly and Nob1p association.

    Evidence Yeast genetics, ribosome fractionation, and rRNA processing analysis

    PMID:20419091

    Open questions at the time
    • Step at which processing is blocked not defined
    • Mechanistic link between C-terminus and Nob1p activity unresolved
  12. 2015 Medium

    How HCV co-opts the 40S subunit was being defined; the RPS5 β-hairpin was shown to bind HCV IRES domains II and IV and to be required for 80S assembly and IRES-driven translation, with knockdown selectively inhibiting viral translation.

    Evidence Modelling, UV cross-linking with IRES mutants, siRNA silencing, and 80S complex assays (building on 2001 cross-linking/IP identification)

    PMID:11331271 PMID:25712089

    Open questions at the time
    • High-resolution structure of the RPS5–IRES contact not determined
    • Selectivity over cellular mRNAs only partially characterized
  13. 2020 Medium

    An extraribosomal role in fibrosis was unknown; RPS5 was shown to suppress fibrogenic Akt and p38 signaling, with epistasis placing RPS5 upstream of these kinases in hepatic stellate and cardiac fibroblasts.

    Evidence Overexpression/knockdown with phospho-signaling readouts, constitutively active p38 rescue, and in vivo fibrosis models

    PMID:24668691 PMID:28880271 PMID:32694761

    Open questions at the time
    • Direct molecular link between RPS5 and the kinases not established
    • Whether ribosomal or free RPS5 mediates the effect unresolved
  14. 2019 Medium

    Whether RPS5 paralogs confer specialized ribosome function was untested; Drosophila RpS5b was shown to be required for oogenesis and to associate with a distinct, metabolism-enriched mRNA pool, indicating paralog-specific ribosome specialization.

    Evidence Drosophila genetic deletion, RNA immunoprecipitation, and ribo-seq

    PMID:31551467 PMID:34495316

    Open questions at the time
    • Mechanism of paralog-specific mRNA selection unknown
    • Relevance to mammalian RPS5 not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How extraribosomal RPS5 mechanistically connects to Akt/p38 signaling and whether the human protein supports specialized, mRNA-selective ribosomes remain unresolved.
  • No direct binding partner identified linking RPS5 to Akt or p38
  • No human evidence for paralog-style ribosome specialization
  • No atomic structure of the human RPS5-eIF2α or RPS5-IRES interface

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 5 GO:0005198 structural molecule activity 3 GO:0045182 translation regulator activity 3
Localization
GO:0005840 ribosome 3 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1643685 Disease 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-8953854 Metabolism of RNA 1
Partners
EIF2EIF2ALPHAEIF3NOB1RIMJ
Complex memberships
40S ribosomal subunit43S pre-initiation complex

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 Human ribosomal protein S5 (rpS5) directly binds the internal ribosome entry site (IRES) of hepatitis C virus RNA on the 40S ribosomal subunit, as demonstrated by UV cross-linking followed by immunoprecipitation with anti-rpS5 antibodies, identifying rpS5 as the ~25 kDa protein critical for positioning HCV RNA on the 40S subunit during translation initiation. UV cross-linking, immunoprecipitation with specific antibodies against rpS5 and rpS9 The Journal of biological chemistry Medium 11331271
2015 The beta-hairpin structure of human/mammalian RPS5 (within the 40S exit channel) is required for binding to HCV IRES domains II and IV; mutations disrupting this interaction drastically reduce 80S complex formation and IRES-driven translation. Partial silencing of RPS5 preferentially inhibits HCV RNA translation with minimal effect on global translation. Computational modelling, RNA-protein interaction studies, UV cross-linking with IRES mutants, siRNA silencing, 80S complex formation assays Nucleic acids research Medium 25712089
2015 The β-hairpin of yeast Rps5/uS7 (β-strand-1 and C-terminal residues) protrudes into the 40S mRNA exit-channel and is essential for efficient and accurate AUG start codon recognition; substitutions in β-strand-1 reduce TC binding to the PIN (closed) conformation of reconstituted 43S·mRNA complexes in vitro, cause leaky scanning of AUGs, and lower initiation fidelity by reducing eIF1 levels. Genetic mutagenesis in yeast, in vitro reconstitution of 43S·mRNA complexes, TC binding assays, translation reporter assays eLife High 26134896
2017 The interface between 40S exit channel protein uS7/Rps5 and eIF2α is remodeled during start codon recognition; uS7 substitutions disrupting eIF2α contacts favored in the open PIC complex increase initiation at suboptimal sites and stabilize TC binding at UUG codons (inappropriate closed-state rearrangement), whereas uS7-D215 substitutions perturbing the closed-state uS7-eIF2α interface confer hyperaccuracy and accelerated TC dissociation, demonstrating that the uS7/eIF2α interface sequentially stabilizes open then closed PIC conformations to promote accurate AUG selection. Yeast genetics (in vivo initiation fidelity assays), in vitro PIC reconstitution with TC binding/dissociation measurements, cryo-EM-guided mutagenesis eLife High 28169832
2007 The negatively charged 21-amino-acid N-terminal extension unique to fungal rpS5 (absent in human rpS5) modulates translation elongation fidelity and IRES activity; replacement of yeast rpS5 with human rpS5 (lacking this extension) in viable yeast causes moderate increases in +1 and -1 programmed frameshifting, hyperaccurate UAA stop codon recognition, reduced polysomal association of eEF3 and eEF1A, and enhanced CrPV IRES–ribosome interaction. Yeast strain replacement genetics, polysome profiling, frameshifting reporter assays, stop codon read-through assays, direct binding assay of CrPV IRES to mutant ribosomes RNA (New York, N.Y.) Medium 17901157
2009 N-terminal truncation analysis of yeast rpS5 shows that deletion of up to 30 N-terminal amino acids is tolerated with moderate growth defects, but deletion of 46 N-terminal amino acids severely impairs growth; truncations reduce the ability of 40S subunits to function in translation and specifically decrease recruitment of initiation factors eIF3 and eIF2. Yeast strain construction with truncated rpS5 variants, growth rate measurement, biochemical analysis of initiation factor association Nucleic acids research Medium 19969550
2010 Yeast rpS5 plays a local structural role at the head/platform interface of the 40S subunit: a variant lacking the seven C-terminal amino acids assembles largely normal head domains that export to the cytoplasm, but 3′ processing of 18S rRNA precursors is inhibited despite association with the endonuclease Nob1p, indicating that the C-terminus of rpS5 is required for efficient 18S rRNA 3′ end maturation. Yeast genetics, ribosome fractionation, rRNA processing analysis, Nob1p association assay PloS one Medium 20419091
2008 RimJ (N-acetyltransferase of E. coli S5) suppresses cold-sensitivity, ribosome biogenesis defects, and translational fidelity defects caused by the S5(G28D) mutation even when devoid of acetyltransferase activity; RimJ associates with pre-30S subunits, indicating it acts as a ribosome assembly factor independently of its acetylation role. Extragenic suppressor screen, ribosome profile analysis, mRNA misreading assays, acetyltransferase-dead mutant complementation, pre-30S association assay Molecular microbiology Medium 18466225
2006 A single G28D mutation in E. coli S5, spatially remote from previously known ram mutations, produces spectinomycin resistance, cold sensitivity, enhanced +1 and -1 frameshifting and nonsense suppression, and altered 16S rRNA folding, without affecting translation initiation, revealing that translational fidelity can be regulated through multiple distinct regions of S5. Site-directed mutagenesis, frameshifting and read-through reporter assays, ribosome profiling, spectinomycin resistance assay RNA (New York, N.Y.) Medium 17053085
2014 RPS5 overexpression in hepatic stellate cells (HSCs) causes dephosphorylation of Akt at Ser473 and Thr308 and subsequent dephosphorylation of GSK3β and P70S6K, thereby preventing HSC activation; Rps5 knockdown aggravates experimental hepatic fibrosis while RPS5 overexpression alleviates it, and RPS5 protein levels are reduced in transdifferentiated HSCs, fibrotic livers, and human cirrhosis. RPS5 cDNA overexpression and shRNA knockdown in HSCs, Western blot for phospho-Akt/GSK3β/P70S6K, in vivo fibrosis models (DMN, BDL), human tissue analysis Hepatology (Baltimore, Md.) Medium 24668691
2017 RPS5 is identified as a direct binding target of the matrine derivative MASM (M19); RPS5 overexpression inhibits osteoclastogenesis via suppression of PI3K/Akt, NF-κB, and MAPKs pathways; RPS5 silencing partially reverses M19's inhibitory effects on osteoclastogenesis, with Akt playing the dominant downstream role. Target identification by compound pulldown, RPS5 overexpression and siRNA knockdown in RAW264.7 cells, pathway analysis by Western blot, RANKL-induced osteoclastogenesis assays, OVX mouse model Cell death & disease Medium 28880271
2020 RPS5 upregulation mediates matrine-induced inhibition of p38 activation and cardiac fibrogenesis; constitutively active p38 overexpression abolishes matrine's protective effects, placing RPS5 upstream of p38 in the signaling cascade that controls cardiac fibroblast proliferation, migration, and collagen production. Matrine treatment in aortic-banding and isoprenaline mouse models, RPS5 overexpression, constitutively active p38 rescue experiments, in vitro cardiac fibroblast assays Acta pharmacologica Sinica Medium 32694761
2008 Constitutive overexpression of RPS5 in murine erythroleukemia (MEL) cells delays both onset of erythroid differentiation and G1/G0 cell cycle arrest, and modulates cyclin-dependent kinase levels (CDK2, CDK4, CDK6), indicating that RPS5 protein level regulates the timing of cell cycle exit during differentiation. Stable transfection of MEL cells with RPS5 cDNA, flow cytometry cell cycle analysis, hemoglobin/differentiation assays, Western blot for CDKs Journal of cellular biochemistry Low 18288641
1992 Crystal structure of E. coli ribosomal protein S5 reveals two distinct alpha/beta domains with structural similarities to other RNP proteins; accuracy-impairing (ram) mutations map to the C-terminal domain proposed to organize rRNA at the decoding region, while spectinomycin-resistance mutations map to the N-terminal domain proposed to interact directly with the rRNA helix that binds spectinomycin. X-ray crystallography, structure/function analysis correlated with known mutation phenotypes Nature High 1508272
1998 High-resolution crystal structures of E. coli S5 show that ram (translational fidelity) mutations cluster in the C-terminal half (proposed to organize decoding-region RNA structures) and spectinomycin-resistance mutations cluster in the N-terminal half (proposed to directly contact the spectinomycin-binding RNA helix), with both sets of mutations within putative RNA-binding sites. High-resolution X-ray crystallography, mapping of known mutations onto structure Journal of molecular biology High 9642068
1988 Chemical probing of E. coli 30S subunits shows that ribosomal protein S5 assembly protects specific residues in the 900 stem/loop and 5'-terminal regions of 16S rRNA, regions also protected by S12 and associated with streptomycin binding and class III tRNA protection, indicating S5 contacts functionally important rRNA elements near the decoding center. Chemical footprinting of 16S rRNA in reconstituted 30S subunits with individual protein additions Journal of molecular biology Medium 2459389
1987 RNA-protein cross-linking in E. coli 30S subunits maps S5 cross-links to the 5'-terminal tetranucleotide of 16S rRNA and to positions 559-561 of 16S rRNA, defining two distinct contact sites of S5 with rRNA. Photo-affinity cross-linking with methyl p-azidophenyl acetimidate, RNA-protein complex isolation, nucleotide mapping Nucleic acids research Medium 2437527
1999 Directed hydroxyl radical probing using Fe(II)-derivatized cysteine mutants of S5 reconstituted into 30S subunits and 70S ribosomes maps the rRNA neighborhood of three S5 positions; in 70S ribosomes, Fe(II)-C99-S5 cleaves 23S rRNA in the 1690-1770 region of domain IV, demonstrating that S5 at the subunit interface contacts the large subunit rRNA. Fe(II)-EDTA tethered hydroxyl radical probing, recombinant protein reconstitution of 30S subunits, 70S ribosome purification Journal of molecular biology Medium 9973556
1990 Yeast SUP44 (ribosomal protein S4 in yeast, the functional ortholog of E. coli S5/ram protein) encodes a protein 26% identical to E. coli S5; a suppressor mutation in SUP44 maps near the region corresponding to known E. coli S5 ram mutation sites, demonstrating functional conservation of translational fidelity regulation between prokaryotes and eukaryotes. Gene cloning, sequencing, suppressor mutation mapping, sequence homology analysis Molecular and cellular biology Medium 2247072
2014 Mutagenesis screen of E. coli rpsE (S5) identifies both error-increasing (ram) and error-restrictive mutations; accuracy-modulating mutations cluster at the S4-S5 interface and at distant sites, and C-terminal truncations of S4 that destabilize the S4-S5 interface all produce ram phenotypes, consistent with the domain closure model for tRNA selection. Site-directed and random mutagenesis, frameshifting and read-through reporter assays, antibiotic resistance profiling Journal of bacteriology Medium 25548247
2019 In Drosophila, RpS5b (a paralog of RpS5a) is required for oogenesis; females lacking RpS5b show widespread apoptosis and developmental defects in mid-oogenesis. RpS5b-associated ribosomes preferentially co-immunoprecipitate mRNAs enriched for mitochondrial electron transport and metabolic GO terms, distinct from the broader mRNA spectrum associated with RpS5a, indicating paralog-specific ribosome-mRNA associations. Genetic deletion of RpS5b in Drosophila, immunoprecipitation of RpS5a/b with mRNA co-purification (RIP), mitochondrial fractionation, proteomics Scientific reports Medium 31551467
2021 Drosophila RpS5b promotes germ cell and follicle cell differentiation during oogenesis; loss of RpS5b increases rRNA transcription and overall protein synthesis, causes microtubule-based defects, and mislocalizes Delta and Mindbomb1, leading to failure of Notch pathway activation in posterior follicle cells. RpS5b deletion genetics, ribo-seq, rRNA transcription assays, immunofluorescence of Delta/Mindbomb1, Notch pathway reporter assays Development (Cambridge, England) Medium 34495316
1987 The rimJ gene of E. coli encodes an N-acetyltransferase that specifically acetylates the N-terminal alanine of ribosomal protein S5; the gene was cloned, sequenced, and the enzyme activity confirmed by protein electrophoresis in maxicells. Gene cloning, nucleotide sequencing, maxicell protein expression, insertional mutagenesis, transcript size analysis Molecular & general genetics : MGG Medium 2828880

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. The EMBO journal 232 7628456
2008 A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proceedings of the National Academy of Sciences of the United States of America 176 18678896
2012 Structure-function analysis of the coiled-coil and leucine-rich repeat domains of the RPS5 disease resistance protein. Plant physiology 170 22331412
1998 A critical role for the S4-S5 intracellular loop in domain IV of the sodium channel alpha-subunit in fast inactivation. The Journal of biological chemistry 155 9422778
1992 The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA. Nature 136 1508272
2001 The S4-S5 linker couples voltage sensing and activation of pacemaker channels. Proceedings of the National Academy of Sciences of the United States of America 132 11553787
1987 Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12. Molecular & general genetics : MGG 126 2828880
2020 Matrine attenuates pathological cardiac fibrosis via RPS5/p38 in mice. Acta pharmacologica Sinica 106 32694761
2002 Structural and functional role of the extracellular s5-p linker in the HERG potassium channel. The Journal of general physiology 102 12407082
1996 N-type inactivation and the S4-S5 region of the Shaker K+ channel. The Journal of general physiology 99 8882863
1988 Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA. Journal of molecular biology 94 2459389
2001 Ribosomal protein S5 interacts with the internal ribosomal entry site of hepatitis C virus. The Journal of biological chemistry 91 11331271
2021 Exosomes from hypoxic pre-treated ADSCs attenuate acute ischemic stroke-induced brain injury via delivery of circ-Rps5 and promote M2 microglia/macrophage polarization. Neuroscience letters 72 34896256
1996 Monoclonal antibodies Ki-S3 and Ki-S5 yield new data on the 'Ki-67' proteins. Cell proliferation 71 8883465
2019 Building a custom large-scale panel of novel microhaplotypes for forensic identification using MiSeq and Ion S5 massively parallel sequencing systems. Forensic science international. Genetics 67 31835179
2019 Sirtuin-1/Mitochondrial Ribosomal Protein S5 Axis Enhances the Metabolic Flexibility of Liver Cancer Stem Cells. Hepatology (Baltimore, Md.) 66 30901096
2015 The Domain II S4-S5 Linker in Nav1.9: A Missense Mutation Enhances Activation, Impairs Fast Inactivation, and Produces Human Painful Neuropathy. Neuromolecular medicine 66 25791876
1995 Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs. Biochimica et biophysica acta 66 7772601
2010 Conserved residues within the putative S4-S5 region serve distinct functions among thermosensitive vanilloid transient receptor potential (TRPV) channels. The Journal of biological chemistry 63 21044960
2002 Impairment of slow inactivation as a common mechanism for periodic paralysis in DIIS4-S5. Neurology 63 11971097
1999 Activation and inactivation of the voltage-gated sodium channel: role of segment S5 revealed by a novel hyperkalaemic periodic paralysis mutation. The Journal of neuroscience : the official journal of the Society for Neuroscience 63 10366610
1975 Neutron scattering measurements of separation and shape of proteins in 30S ribosomal subunit of Escherichia coli: S2-S5, S5-S8, S3-S7. Proceedings of the National Academy of Sciences of the United States of America 59 1105567
1990 Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein. Molecular and cellular biology 57 2247072
2014 Bioactive compound reveals a novel function for ribosomal protein S5 in hepatic stellate cell activation and hepatic fibrosis. Hepatology (Baltimore, Md.) 55 24668691
1987 RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate. Nucleic acids research 53 2437527
2011 Sequence variants of the Phytophthora sojae RXLR effector Avr3a/5 are differentially recognized by Rps3a and Rps5 in soybean. PloS one 52 21779316
2005 Mutation of colocalized residues of the pore helix and transmembrane segments S5 and S6 disrupt deactivation and modify inactivation of KCNQ1 K+ channels. The Journal of physiology 50 15649981
2005 Extracellular acid block and acid-enhanced inactivation of the Ca2+-activated cation channel TRPM5 involve residues in the S3-S4 and S5-S6 extracellular domains. The Journal of biological chemistry 50 15731110
2017 Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis. Cell death & disease 48 28880271
2015 PIP₂-dependent coupling is prominent in Kv7.1 due to weakened interactions between S4-S5 and S6. Scientific reports 48 25559286
2011 Demonstration of physical proximity between the N terminus and the S4-S5 linker of the human ether-a-go-go-related gene (hERG) potassium channel. The Journal of biological chemistry 46 21474444
2008 Suppression of a cold-sensitive mutation in ribosomal protein S5 reveals a role for RimJ in ribosome biogenesis. Molecular microbiology 46 18466225
2019 HCMV-encoded US7 and US8 act as antagonists of innate immunity by distinctively targeting TLR-signaling pathways. Nature communications 45 31604943
2015 A new KCNQ1 mutation at the S5 segment that impairs its association with KCNE1 is responsible for short QT syndrome. Cardiovascular research 45 26168993
2013 Pore-forming pyocin S5 utilizes the FptA ferripyochelin receptor to kill Pseudomonas aeruginosa. Microbiology (Reading, England) 45 24217175
1998 Ribosomal proteins S5 and L6: high-resolution crystal structures and roles in protein synthesis and antibiotic resistance. Journal of molecular biology 43 9642068
1975 Genetic position and amino acid replacements of several mutations in ribosomal protein S5 from Escherichia coli. Molecular & general genetics : MGG 42 129673
1999 Determinants of voltage-dependent gating and open-state stability in the S5 segment of Shaker potassium channels. The Journal of general physiology 41 10435999
2020 Pyocin S5 Import into Pseudomonas aeruginosa Reveals a Generic Mode of Bacteriocin Transport. mBio 40 32156826
2002 Human cytomegalovirus US7, US8, US9, and US10 are cytoplasmic glycoproteins, not found at cell surfaces, and US9 does not mediate cell-to-cell spread. Journal of virology 38 11992003
2009 Interactions of H562 in the S5 helix with T618 and S621 in the pore helix are important determinants of hERG1 potassium channel structure and function. Biophysical journal 37 19413965
2006 A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity. RNA (New York, N.Y.) 36 17053085
2019 A ribosomal protein S5 isoform is essential for oogenesis and interacts with distinct RNAs in Drosophila melanogaster. Scientific reports 35 31551467
2007 Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida. Antimicrobial agents and chemotherapy 35 17371823
2011 Human cytomegalovirus US7 is regulated synergistically by two virally encoded microRNAs and by two distinct mechanisms. Journal of virology 34 21900172
2013 Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae. Frontiers in microbiology 33 23847609
2020 RPS5-Mediated Disease Resistance: Fundamental Insights and Translational Applications. Annual review of phytopathology 32 32284014
2018 A Matrine Derivative M54 Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss by Targeting Ribosomal Protein S5. Frontiers in pharmacology 32 29441015
2016 Peptidoglycan recognition protein-S5 functions as a negative regulator of the antimicrobial peptide pathway in the silkworm, Bombyx mori. Developmental and comparative immunology 31 27012996
2008 Thermodynamic and kinetic properties of amino-terminal and S4-S5 loop HERG channel mutants under steady-state conditions. Biophysical journal 31 18222997
2015 The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function. Nucleic acids research 30 25712089
2010 Highly redundant function of multiple AT-rich sequences as core promoter elements in the TATA-less RPS5 promoter of Saccharomyces cerevisiae. Nucleic acids research 29 20805245
2004 Cooperative effect of S4-S5 loops in domains D3 and D4 on fast inactivation of the Na+ channel. The Journal of physiology 29 15459238
2024 Exosomes from adipose-derived stem cells regulate macrophage polarization and accelerate diabetic wound healing via the circ-Rps5/miR-124-3p axis. Immunity, inflammation and disease 28 38888351
2017 The S4---S5 linker - gearbox of TRP channel gating. Cell calcium 28 28416203
2007 Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5. RNA (New York, N.Y.) 28 17901157
2005 Delimitation of the rice wide compatibility gene S5 ( n ) to a 40-kb DNA fragment. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 27 16177904
2024 Characterization and interactions of spoilage of Pseudomonas fragi C6 and Brochothrix thermosphacta S5 in chilled pork based on LC-MS/MS and screening of potential spoilage biomarkers. Food chemistry 26 38330602
2022 DNA methylation testing with S5 for triage of high-risk HPV positive women. International journal of cancer 26 35477862
2006 S5 Lipase: an organic solvent tolerant enzyme. Journal of microbiology (Seoul, Korea) 26 17205035
2003 Expression and purification of human ribosomal proteins S3, S5, S10, S19, and S26. Protein expression and purification 26 12651107
2021 The Drosophila ribosome protein S5 paralog RpS5b promotes germ cell and follicle cell differentiation during oogenesis. Development (Cambridge, England) 25 34495316
2020 Comparison of Illumina MiSeq and the Ion Torrent PGM and S5 platforms for whole-genome sequencing of picornaviruses and caliciviruses. Journal of virological methods 25 32302601
2019 Antagonistic trait of Lactobacillus reuteri S5 against Salmonella enteritidis and assessment of its potential probiotic characteristics. Microbial pathogenesis 25 31604155
2015 Exopolysaccharide of Antarctic bacterium Pseudoaltermonas sp. S-5 induces apoptosis in K562 cells. Carbohydrate polymers 25 25659678
2014 Modulation of decoding fidelity by ribosomal proteins S4 and S5. Journal of bacteriology 25 25548247
2012 Structure and stability of duplex DNA containing (5'S)-5',8-cyclo-2'-deoxyadenosine: an oxidatively generated lesion repaired by NER. Chemical research in toxicology 25 22928555
2005 Role of an S4-S5 linker lysine in the trafficking of the Ca(2+)-activated K(+) channels IK1 and SK3. The Journal of biological chemistry 25 16135513
2022 Targeted S5 RNA sequencing assay for the identification and direct association of common body fluids with DNA donors in mixtures. International journal of legal medicine 24 36333511
2019 Construction of a US7/US8/UL23/US3-deleted recombinant pseudorabies virus and evaluation of its pathogenicity in dogs. Veterinary microbiology 24 31902487
2017 Sulfur Metabolism of Hydrogenovibrio thermophilus Strain S5 and Its Adaptations to Deep-Sea Hydrothermal Vent Environment. Frontiers in microbiology 24 29312214
2015 The β-hairpin of 40S exit channel protein Rps5/uS7 promotes efficient and accurate translation initiation in vivo. eLife 24 26134896
2012 Opposite Effects of the S4-S5 Linker and PIP(2) on Voltage-Gated Channel Function: KCNQ1/KCNE1 and Other Channels. Frontiers in pharmacology 24 22787448
2009 Yeast strains with N-terminally truncated ribosomal protein S5: implications for the evolution, structure and function of the Rps5/Rps7 proteins. Nucleic acids research 24 19969550
2017 Kinetic and thermodynamic properties of alginate lyase and cellulase co-produced by Exiguobacterium species Alg-S5. International journal of biological macromolecules 23 28122206
2008 The potential role of ribosomal protein S5 on cell cycle arrest and initiation of murine erythroleukemia cell differentiation. Journal of cellular biochemistry 23 18288641
2006 The concerted contribution of the S4-S5 linker and the S6 segment to the modulation of a Kv channel by 1-alkanols. Molecular pharmacology 22 16887933
1999 Expression of ribosomal protein S5 cloned gene during differentiation and apoptosis in murine erythroleukemia (MEL) cells. Oncology research 22 10821535
2019 Utility of the Ion S5™ and MiSeq FGx™ sequencing platforms to characterize challenging human remains. Legal medicine (Tokyo, Japan) 21 31499459
2016 Relation among anti-rheumatic drug therapy, CD14(+)CD16(+) blood monocytes and disease activity markers (DAS28 and US7 scores) in rheumatoid arthritis: A pilot study. Pharmacological research 21 27045818
2010 A local role for the small ribosomal subunit primary binder rpS5 in final 18S rRNA processing in yeast. PloS one 21 20419091
1999 Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5. Applied microbiology and biotechnology 21 10422230
1992 The primary structure of rat ribosomal protein S5. A ribosomal protein present in the rat genome in a single copy. The Journal of biological chemistry 21 1460027
1988 The herpes simplex virus type 1 US7 gene product is a 66K glycoprotein and is a target for complement-dependent virus neutralization. The Journal of general virology 21 2833569
2001 Dynamic interaction of S5 and S6 during voltage-controlled gating in a potassium channel. The Journal of general physiology 20 11479343
2000 Cloning and heterologous expression of a sulfur oxygenase/reductase gene from the thermoacidophilic archaeon Acidianus sp. S5 in Escherichia coli. FEMS microbiology letters 19 11111027
1996 p53 expression, proliferation marker Ki-S5, DNA content and serum PSA: possible biopotential markers in human prostatic cancer. Urology 19 8753738
2009 Accuracy modulating mutations of the ribosomal protein S4-S5 interface do not necessarily destabilize the rps4-rps5 protein-protein interaction. RNA (New York, N.Y.) 18 19386726
2001 Requirement for yeast TAF145 function in transcriptional activation of the RPS5 promoter that depends on both core promoter structure and upstream activating sequences. The Journal of biological chemistry 18 11337503
2020 Validation and interpretation of IGH and TCR clonality testing by Ion Torrent S5 NGS for diagnosis and disease monitoring in B and T cell cancers. Practical laboratory medicine 17 33304977
2021 Transcriptomic analysis of Burkholderia cenocepacia CEIB S5-2 during methyl parathion degradation. Environmental science and pollution research international 16 33813711
2021 Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines. Protein expression and purification 16 34752859
2018 S5, a Withanolide Isolated from Physalis Pubescens L., Induces G2/M Cell Cycle Arrest via the EGFR/P38 Pathway in Human Melanoma A375 Cells. Molecules (Basel, Switzerland) 16 30513793
2021 A novel dextranase gene from the marine bacterium Bacillus aquimaris S5 and its expression and characteristics. FEMS microbiology letters 15 33476380
2019 Anti-Ro52 antibody acts on the S5-pore linker of hERG to chronically reduce channel expression. Cardiovascular research 15 30544220
2005 A1152D mutation of the Na+ channel causes paramyotonia congenita and emphasizes the role of DIII/S4-S5 linker in fast inactivation. The Journal of physiology 15 15790667
1999 Probing the rRNA environment of ribosomal protein S5 across the subunit interface and inside the 30 S subunit using tethered Fe(II). Journal of molecular biology 15 9973556
2023 Polysaccharide degradation in Cellvibrionaceae: Genomic insights of the novel chitin-degrading marine bacterium, strain KSP-S5-2, and its chitinolytic activity. The Science of the total environment 14 38070563
2017 Interface between 40S exit channel protein uS7/Rps5 and eIF2α modulates start codon recognition in vivo. eLife 14 28169832
1999 The RRM protein NonA from Drosophila forms a complex with the RRM proteins Hrb87F and S5 and the Zn finger protein PEP on hnRNA. Experimental cell research 14 10585281

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