Affinage

RPL11

Large ribosomal subunit protein uL5 · UniProt P62913

Length
178 aa
Mass
20.3 kDa
Annotated
2026-06-10
88 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPL11 (uL5) is a structural component of the 60S ribosomal subunit that doubles as a stress-responsive regulator of the p53 tumor-suppressor pathway (PMID:17938242, PMID:36435197). During ribosome assembly, RPL11 is recruited together with RPL5 and 5S rRNA into nascent 90S preribosomal particles by the assembly factors Rpf2 and Rrs1, a step required for 27SB pre-rRNA processing and proper 60S maturation (PMID:17938242); loss of RPL11 collapses 60S biogenesis and overall ribosome content, limiting translational capacity and cyclin accumulation (PMID:24061479, PMID:34948282). Upon ribosomal or nucleolar stress, free RPL11 escapes the nucleolus and binds MDM2 directly: a 2.4 Å crystal structure shows MDM2 engaging RPL11 through its acidic domain and a C4 zinc finger, mimicking 28S rRNA binding, and MDM2 mutants unable to bind RPL11 fail to activate p53 (PMID:26220995, PMID:21903592). This interaction blocks MDM2 E3 ubiquitin ligase activity toward p53 and additionally recruits RPL11 to p53 target promoters, where it relieves MDM2-mediated transcriptional repression and licenses p300/CBP-dependent p53 K382 acetylation (PMID:22081073). Nucleolar release of RPL11 is gated by retention factors PICT1/GLTSCR2 and RRS1 and tuned by post-translational modification—NEDDylation promotes and is antagonized by SUMOylation, while USP47-mediated deubiquitination and ATM-driven PICT1 degradation control the free pool (PMID:21804542, PMID:34433556, PMID:22081073, PMID:30024791, PMID:35205710, PMID:27829214). This RPL11–MDM2–p53 checkpoint is the mechanistic basis of anemia in RPL11-haploinsufficient Diamond-Blackfan anemia mouse models: erythroid differentiation defects are rescued by lowering p53 dosage or by knocking in an RP-binding-null MDM2 (PMID:26489471, PMID:36435197). The pathway is dysregulated by oncoproteins and regulators including GRWD1, RPS27a, PRAS40, and MeCP2 that limit RPL11–MDM2 engagement (PMID:27856536, PMID:35073964, PMID:24704832, PMID:32483207). Beyond p53, extra-ribosomal RPL11 directly inhibits PPARα transcriptional activity (PMID:16280383) and cooperates with RBM10 to promote ubiquitin-dependent degradation of c-Myc, suppressing its oncogenic function (PMID:38032932).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2005 Medium

    Established that RPL11 acts beyond the ribosome as a direct transcriptional modulator, the first extra-ribosomal function defined for it.

    Evidence Yeast/mammalian two-hybrid, GST pull-down and reporter assays mapping RPL11 binding to the PPARα hinge (D-domain)

    PMID:16280383

    Open questions at the time
    • Physiological context and target-gene scope of PPARα inhibition undefined
    • Selectivity over PPARβ/γ explained only by binding, not structurally
  2. 2007 High

    Defined how RPL11 is incorporated into the ribosome, identifying the assembly factors that load it onto pre-rRNPs.

    Evidence In vitro binding, Co-IP and pre-rRNA processing analysis with Rpf2/Rrs1 depletion in yeast

    PMID:17938242

    Open questions at the time
    • Human ortholog assembly step not directly demonstrated here
    • Does not address the extra-ribosomal free pool
  3. 2009 High

    Resolved how impaired 40S biogenesis still signals through RPL11, showing selective 5'-TOP translation raises free RPL11 even without nucleolar disruption.

    Evidence siRNA, partial hepatectomy mouse model, p53 reporters and polysome fractionation

    PMID:19287375

    Open questions at the time
    • Mechanism coupling rpS6 loss to TOP-mRNA selectivity incomplete
    • Direct measurement of nascent free RPL11 partitioning limited
  4. 2011 High

    Identified nucleolar retention as the upstream switch controlling RPL11 availability and the modification/promoter steps that translate stress into p53 activation.

    Evidence PICT1-knockout mice/ES cells with Co-IP and ubiquitination assays; ChIP showing RPL11 promoter recruitment and NEDDylation dependence

    PMID:21804542 PMID:22081073

    Open questions at the time
    • How nucleolar release is triggered at the molecular level not fully resolved
    • NEDDylation site/enzyme specificity not mapped
  5. 2011 High

    Pinpointed the molecular contacts of RPL11 with the MDM2 zinc finger required to suppress its E3 ligase activity.

    Evidence In vitro pull-down, HDX-MS, circular dichroism and mutagenesis

    PMID:21903592

    Open questions at the time
    • Conformational dynamics inferred biophysically, not yet structural
    • Stoichiometry with 5S rRNA/RPL5 not addressed
  6. 2013 Medium

    Distinguished RPL11/RPL5 from other ribosomal proteins by showing their loss limits translation without triggering a p53 arrest, clarifying their dual roles.

    Evidence siRNA in primary fibroblasts with flow cytometry, polysome fractionation and cyclin measurements; v-erbA-induced RPL11-deficient ribosomes by proteomics

    PMID:23563180 PMID:24061479

    Open questions at the time
    • Why RPL11/RPL5 loss uniquely bypasses arrest mechanistically unclear
    • Functional consequences of RPL11-deficient specialized ribosomes for specific mRNAs limited
  7. 2015 High

    Provided the atomic-resolution basis for RPL11–MDM2 recognition, showing MDM2 mimics 28S rRNA and that a specific zinc finger dictates binding and p53 activation.

    Evidence X-ray crystallography at 2.4 Å with structure-guided mutagenesis and cell-based p53 assays

    PMID:26220995

    Open questions at the time
    • Structure of full ternary RPL11/5S/MDM2 assembly not solved
    • Does not capture in-cell regulation by modification
  8. 2015 Low

    Began linking RPL11 to c-Myc control, indicating a second extra-ribosomal tumor-suppressive axis sensitive to 5S rRNA levels.

    Evidence Co-IP and miRNA/siRNA manipulation with proliferation assays in esophageal carcinoma cells

    PMID:26606907

    Open questions at the time
    • Single Co-IP without reciprocal/structural validation
    • Direct vs. indirect RPL11–c-Myc contact unresolved
  9. 2015 High

    Established RPL11 haploinsufficiency as a cause of anemia via the ribosomal stress–p53 checkpoint, modeling Diamond-Blackfan anemia.

    Evidence Conditional heterozygous Rpl11 mice with bone marrow transplantation, p53 assays and flow cytometry

    PMID:26489471

    Open questions at the time
    • Whether elevated basal c-MYC contributes independently to anemia not isolated
    • Cell-autonomy across erythroid stages partially defined
  10. 2016 Medium

    Identified oncoprotein and DNA-damage inputs that gate RPL11–MDM2 engagement, expanding regulation beyond ribosomal stress.

    Evidence Co-IP, ubiquitination and domain mapping for GRWD1 competition; ATM phospho-site mutagenesis driving PICT-1 degradation

    PMID:27829214 PMID:27856536

    Open questions at the time
    • Quantitative contribution of each regulator in vivo unknown
    • Crosstalk hierarchy among GRWD1/PICT1/ATM not integrated
  11. 2018 Medium

    Defined modification crosstalk—SUMOylation antagonizing NEDDylation—as a tunable control over RPL11 nucleolar exit and p53 activation.

    Evidence Co-IP, immunofluorescence, siRNA and SUMO/NEDD8 modification assays

    PMID:30024791

    Open questions at the time
    • SUMO acceptor sites and ligase specificity not mapped
    • Single-lab evidence without structural confirmation
  12. 2020 Medium

    Broadened the network of factors that sequester RPL11 from MDM2, including a protein partner, a transcriptional repressor, and a lncRNA scaffold.

    Evidence GST pull-down/Co-IP for RPS27a; ChIP for MeCP2 repression; RNA-IP for PiHL-GRWD1-RPL11; ubiquitination assays throughout

    PMID:31903119 PMID:32483207 PMID:35073964

    Open questions at the time
    • Relative importance of these regulators across tissues unknown
    • Most rest on single-lab Co-IP/RNA-IP without reciprocal structural data
  13. 2021 Medium

    Confirmed RRS1 as a nucleolar retention factor for RPL11 with tumor-relevant consequences, and mapped the system-wide translational impact of RPL11 loss.

    Evidence Co-IP, fractionation, ubiquitination and in vivo tumor assays for RRS1; parallel transcriptome/translatome RNA-seq with ribosome fractionation for RPL11 deficiency

    PMID:34433556 PMID:34948282

    Open questions at the time
    • Direct contribution of altered specific-mRNA translation to phenotype unresolved
    • Single-lab datasets
  14. 2022 High

    Placed the RP–MDM2–p53 axis genetically as the cause of RPL11-haploinsufficient anemia and added USP47 deubiquitination as a nucleolar-retention control.

    Evidence p53/MDM2 RP-binding mutant knock-in epistasis in mice; Co-IP, localization and USP47 catalytic-mutant rescue

    PMID:35205710 PMID:36435197

    Open questions at the time
    • How USP47 ubiquitination signal is read for nucleolar retention not fully defined
    • Therapeutic exploitability of the MDM2 RP-binding pocket untested
  15. 2023 Medium

    Solidified the c-Myc suppression axis, showing RPL11 with RPL5 binds RBM10 to drive c-Myc ubiquitin-dependent degradation, disrupted by a cancer mutation.

    Evidence Reciprocal Co-IP, ubiquitination assays, domain mapping and the cancer-derived RBM10-I316F mutant in growth assays

    PMID:38032932

    Open questions at the time
    • Whether c-Myc and p53 axes are coordinated under the same stress signals unclear
    • Single-lab evidence without structural detail of the RBM10 complex

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the various nucleolar retention factors, modifications, and oncoprotein inputs are integrated to set the free RPL11 threshold, and how the ribosomal versus extra-ribosomal pools are physically partitioned, remains unresolved.
  • No unified structural model of the regulated RPL11/5S/RPL5 module
  • Quantitative dynamics of nucleolar-to-nucleoplasmic flux not measured in vivo
  • Coordination of p53 and c-Myc outputs under physiological stress undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0098772 molecular function regulator activity 3 GO:0003723 RNA binding 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005730 nucleolus 5 GO:0005654 nucleoplasm 3 GO:0005840 ribosome 3
Pathway
R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-1643685 Disease 2 R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
GRWD1MDM2PICT1PPARAPRAS40RBM10RPS27ARRS1
Complex memberships
60S ribosomal subunitRPL5-RPL11-5S rRNA module

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2015 Crystal structure of human MDM2 complexed with RPL11 at 2.4 Å resolution reveals that MDM2 extensively interacts with RPL11 through an acidic domain and two zinc fingers; formation of the complex induces substantial conformational changes in both proteins; MDM2 mimics 28S rRNA binding to RPL11; the C4 zinc finger of MDM2 determines RPL11 binding but not binding to MDMX; MDM2 mutants unable to bind RPL11 fail to induce p53 activation in cells. X-ray crystallography (2.4 Å), structure-guided mutagenesis, cell-based p53 activation assays Genes & development High 26220995
2011 PICT1 (GLTSCR2) binds RPL11 in the nucleolus and retains it there; loss of PICT1 releases RPL11 from nucleoli, increasing RPL11-MDM2 binding, blocking MDM2-mediated ubiquitination of p53, and causing p53-dependent G1 arrest and apoptosis even without DNA damage. Co-immunoprecipitation, Pict1-knockout mice and ES cells, nucleolar fractionation/immunofluorescence, ubiquitination assay Nature medicine High 21804542
2009 Impairment of 40S ribosome biogenesis (via rpS6 depletion) does not disrupt nucleolar integrity yet still induces p53 via RPL11; the mechanism involves selective upregulation of 5'-TOP mRNA translation (including rpL11 mRNA) despite global translational suppression, increasing free RPL11 protein. siRNA knockdown, mouse liver partial hepatectomy model, p53 reporter assays, polysome fractionation Nature cell biology High 19287375
2011 RPL11 is rapidly recruited to promoter sites of p53-regulated genes upon nucleolar stress (actinomycin D); RPL11 is required for recruitment of p300/CBP co-activators and p53 K382 acetylation; NEDDylation of RPL11 and its direct binding to MDM2 are critical for promoter recruitment; binding of RPL11 to MDM2 at the promoter relieves MDM2-mediated transcriptional repression of p53. Chromatin immunoprecipitation (ChIP), co-immunoprecipitation, siRNA knockdown, promoter reporter assays Oncogene High 22081073
2011 RPL11 interacts with the zinc finger domain of MDM2 via hydrophilic (basic) residues; hydrogen-deuterium exchange mass spectrometry and circular dichroism confirmed direct contact between RPL11 and the MDM2 zinc finger; single mutations of non-cysteine residues in the zinc finger disrupt binding; basic residues in RPL11 are essential for stable MDM2 binding and suppression of MDM2 E3 ligase activity toward p53. In vitro pull-down, hydrogen-deuterium exchange MS, circular dichroism, computational modeling, mutagenesis The Journal of biological chemistry High 21903592
2007 Assembly factors Rpf2 and Rrs1 are required to recruit rpL11 (along with rpL5 and 5S rRNA) into nascent 90S preribosomal particles; in the absence of Rpf2/Rrs1, rpL11 fails to associate with pre-rRNPs, 27SB pre-rRNA processing is blocked, and abortive 66S particles are prematurely released from the nucleolus. In vitro binding assays, co-immunoprecipitation, pre-rRNA processing analysis, genetic depletion in yeast Genes & development High 17938242
2013 Loss of RPL5/RPL11 abrogates 60S ribosome biogenesis and translational capacity but, unlike loss of other 60S ribosomal proteins, does NOT trigger a p53-dependent cell cycle checkpoint; instead, depletion reduces ribosome content and suppresses cyclin accumulation at the translational level, impairing cell cycle progression without arrest. siRNA knockdown in primary human lung fibroblasts, flow cytometry, polysome fractionation, cyclin protein/mRNA measurements Molecular and cellular biology High 24061479
2016 GRWD1 physically interacts with RPL11; overexpression of GRWD1 competitively inhibits the RPL11-MDM2 interaction via its N-terminal acidic domain, alleviating RPL11-mediated suppression of MDM2 ubiquitin ligase activity toward p53; silencing GRWD1 enhances p53 induction by nucleolar stress. Co-immunoprecipitation, MDM2 ubiquitin ligase assay, siRNA/overexpression, immunofluorescence EMBO reports High 27856536
2018 RPL11 is covalently modified by SUMO1 and SUMO2; SUMOylation negatively modulates NEDDylation of RPL11; SUMOylation promotes RPL11 translocation out of the nucleolus; the SUMO-conjugating enzyme Ubc9 is required for RPL11-mediated p53 activation; SUMOylation is triggered by ribosomal stress. Co-immunoprecipitation, immunofluorescence, siRNA knockdown, SUMO/NEDD8 modification assays FASEB journal Medium 30024791
2014 Akt/mTORC1-mediated phosphorylation of PRAS40 at T246 and S221 promotes nuclear-specific association of PRAS40 with RPL11; silencing PRAS40 induces p53 upregulation in an RPL11-dependent manner; PRAS40T246A (RPL11-binding-null mutant) cannot rescue this effect; PRAS40 negatively regulates the RPL11-HDM2-p53 pathway and suppresses p53-mediated cellular senescence. Co-immunoprecipitation, siRNA knockdown, phospho-site mutagenesis, cellular senescence assays Oncogene Medium 24704832
2020 RPS27a directly binds RPL11 (confirmed by GST pull-down); RPS27a knockdown weakens RPS27a-RPL11 interaction and enhances RPL11-MDM2 binding, thereby inhibiting MDM2-mediated ubiquitination and degradation of p53; p53 stabilization by RPS27a knockdown is RPL11-dependent. GST pull-down, co-immunoprecipitation, in vitro ubiquitination assay, siRNA knockdown Journal of experimental & clinical cancer research Medium 35073964
2020 MeCP2 represses RPL11 (and RPL5) transcription by binding to their promoter regions; RPL11 overexpression suppresses breast cancer cell proliferation and induces apoptosis; RPL11 suppresses MDM2-mediated ubiquitination of p53 through direct MDM2 binding. ChIP assay, co-immunoprecipitation, overexpression/knockdown, ubiquitination assay Oncogenesis Medium 32483207
2021 RRS1 retains RPL11 in the nucleolus; RRS1 overexpression attenuates ribosomal stress by preventing RPL11 release, potentiating MDM2-mediated ubiquitination and degradation of p53; RRS1 knockdown releases RPL11 from the nucleolus, increases RPL11-MDM2 interaction, and activates p53. Co-immunoprecipitation, immunofluorescence/nucleolar fractionation, ubiquitination assay, in vitro and in vivo tumor growth assays Science advances Medium 34433556
2022 miR-101-3p targets USP47, a deubiquitinase for RPL11; USP47-mediated deubiquitination of RPL11 retains it in the nucleolus; inhibition of USP47 by miR-101-3p promotes RPL11 translocation to the nucleoplasm, enhancing RPL11-MDM2 interaction and p53 stabilization; catalytically inactive USP47 cannot rescue these effects. Co-immunoprecipitation, immunofluorescence, miRNA overexpression, USP47 catalytic mutant Cancers Medium 35205710
2005 RPL11 directly interacts with PPARα within the D-domain (hinge region) of PPARα, confirmed by yeast two-hybrid, mammalian two-hybrid, and in vitro pull-down; RPL11 co-transfection produces ligand-dependent inhibition of PPARα transcriptional activity associated with decreased PPRE DNA binding; RPL11 does not interact with PPARβ or PPARγ. Yeast two-hybrid, mammalian two-hybrid, GST pull-down, co-transfection reporter assay, siRNA Toxicological sciences Medium 16280383
2015 Down-regulation of 5S rRNA (by miR-150/miR-383) intensifies the interaction between rpL11 and c-Myc, attenuating c-Myc oncogenic transcriptional activity and inhibiting esophageal squamous carcinoma cell proliferation. Co-immunoprecipitation, miRNA overexpression, siRNA knockdown, cell proliferation assays FEBS letters Low 26606907
2023 RBM10 directly binds to c-Myc and promotes its ubiquitin-dependent degradation; RPL11 (uL5) and RPL5 (uL18) directly bind RBM10 and boost this c-Myc suppression; cancer-derived mutant RBM10-I316F fails to bind RPL11/RPL5, abrogating c-Myc inactivation. Co-immunoprecipitation, ubiquitination assay, domain-mapping mutagenesis, cell growth/proliferation assays Proceedings of the National Academy of Sciences of the United States of America Medium 38032932
2013 v-erbA oncogene expression generates ribosomes devoid of RPL11 in avian erythroid progenitors; immunoprecipitation confirmed ribosome-associated RPL11 is reduced; these specialized RPL11-deficient ribosomes are associated with altered translational efficiency of specific mRNAs (e.g., HSP70). 2D-DIGE proteomics, co-immunoprecipitation of ribosomes, polyribosome fractionation Oncogene Medium 23563180
2015 Rpl11 heterozygous deletion in adult mice causes anemia with decreased erythroid progenitors and defective erythroid maturation; fibroblasts and hematopoietic tissue from heterozygous Rpl11 mice show impaired p53 activation upon ribosomal stress or DNA damage and elevated basal c-MYC levels; total/partial RPL11 loss compromises the ribosomal stress-p53 checkpoint. Conditional knockout mice, bone marrow transplantation, p53 activation assays, flow cytometry Cell reports High 26489471
2022 RPL11 haploinsufficiency in adult mice activates p53 in hematopoietic tissues and impedes erythroid precursor differentiation, causing anemia; reducing p53 dosage (p53 heterozygous deletion) rescues erythropoietic precursor differentiation and restores normal red blood cell levels; introducing an RP-binding mutation in MDM2 (blocking RPL11-MDM2 interaction) prevents p53 activation and rescues the anemia, establishing the RP-MDM2-p53 pathway as the mechanistic basis. Conditional knockout mice, p53/MDM2 RP-binding mutant knock-in, flow cytometry, genetic epistasis The Journal of biological chemistry High 36435197
2021 RPL11 deficiency in HEK293T cells causes an almost proportional decrease in 80S ribosomes; transcriptomic analysis shows hundreds of differentially expressed genes at transcriptome and translatome levels; up-regulated genes are associated with rRNA processing, splicing, translation and DNA repair; down-regulated genes are membrane protein genes; genes with altered translational efficiency depend on coding sequence length. RNA interference, RNA-seq of total and polysome-associated mRNA, ribosome fractionation International journal of molecular sciences Medium 34948282
2014 Perturbation of RNA Pol I transcription (via HEATR1 depletion) causes nucleolar disruption and activates p53 in an RPL5/RPL11-dependent manner; p53-dependent cell cycle arrest upon HEATR1 depletion is rescued by co-depletion of RPL5/RPL11. siRNA knockdown, immunofluorescence, cell cycle analysis, epistasis by double knockdown Cell cycle Medium 29143558
2016 PICT-1 (GLTSCR2) is phosphorylated by ATM at S233/T289 in response to DNA damage; this phosphorylation promotes PICT-1 degradation and releases RPL11 from the nucleolus to the nucleoplasm; released RPL11 increases binding to MDM2 and promotes p53 accumulation and apoptosis in an ATM-dependent manner. Co-immunoprecipitation, immunofluorescence, phospho-site mutagenesis (S233A/T289A and S233D/T289D), ATM inhibitor treatment Oncotarget Medium 27829214
2020 The lncRNA PiHL sequesters RPL11 from MDM2 by enhancing GRWD1-RPL11 complex formation; this promotes MDM2-mediated p53 ubiquitination and degradation, reducing p53 activity in colorectal cancer cells. Co-immunoprecipitation, RNA immunoprecipitation, ubiquitination assay, siRNA knockdown Theranostics Medium 31903119

Source papers

Stage 0 corpus · 88 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 Absence of nucleolar disruption after impairment of 40S ribosome biogenesis reveals an rpL11-translation-dependent mechanism of p53 induction. Nature cell biology 261 19287375
2007 Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes. Genes & development 167 17938242
2011 Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11. Nature medicine 153 21804542
1991 Association of DNA helicase and primase activities with a subassembly of the herpes simplex virus 1 helicase-primase composed of the UL5 and UL52 gene products. Proceedings of the National Academy of Sciences of the United States of America 102 1847509
1992 The six conserved helicase motifs of the UL5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function. Journal of virology 101 1309257
2000 Construction, phenotypic analysis, and immunogenicity of a UL5/UL29 double deletion mutant of herpes simplex virus 2. Journal of virology 96 10933704
1998 Inhibition of herpes simplex virus replication by a 2-amino thiazole via interactions with the helicase component of the UL5-UL8-UL52 complex. Journal of virology 89 9696789
2013 Loss of tumor suppressor RPL5/RPL11 does not induce cell cycle arrest but impedes proliferation due to reduced ribosome content and translation capacity. Molecular and cellular biology 83 24061479
2009 Identification of mutations in the ribosomal protein L5 (RPL5) and ribosomal protein L11 (RPL11) genes in Czech patients with Diamond-Blackfan anemia. Human mutation 80 19191325
2015 Structure of human MDM2 complexed with RPL11 reveals the molecular basis of p53 activation. Genes & development 79 26220995
2009 Diamond-Blackfan anemia: genotype-phenotype correlations in Italian patients with RPL5 and RPL11 mutations. Haematologica 78 19773262
2015 Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis. Cell reports 76 26489471
1996 DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus. The Journal of general virology 73 8811022
2022 Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells. Journal of experimental & clinical cancer research : CR 71 35073964
2012 Primary hematopoietic cells from DBA patients with mutations in RPL11 and RPS19 genes exhibit distinct erythroid phenotype in vitro. Cell death & disease 68 22833095
2011 Recruitment of RPL11 at promoter sites of p53-regulated genes upon nucleolar stress through NEDD8 and in an Mdm2-dependent manner. Oncogene 64 22081073
1994 Simple method of purification and sequencing of a bacteriocin produced by Pediococcus acidilactici UL5. The Journal of applied bacteriology 61 7822227
2020 Long noncoding RNA PiHL regulates p53 protein stability through GRWD1/RPL11/MDM2 axis in colorectal cancer. Theranostics 57 31903119
1992 The UL5 gene of herpes simplex virus type 1: isolation of a lacZ insertion mutant and association of the UL5 gene product with other members of the helicase-primase complex. Journal of virology 53 1309255
1994 The UL8 component of the herpes simplex virus helicase-primase complex stimulates primer synthesis by a subassembly of the UL5 and UL52 components. The Journal of biological chemistry 50 8106478
2016 GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO reports 47 27856536
2014 Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene 46 24704832
1988 UL5, a protein required for HSV DNA synthesis: genetic analysis, overexpression in Escherichia coli, and generation of polyclonal antibodies. Virology 46 2845650
2021 The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts. mBio 42 33947766
2020 MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription. Oncogenesis 40 32483207
2005 Mutations in the putative zinc-binding motif of UL52 demonstrate a complex interdependence between the UL5 and UL52 subunits of the human herpes simplex virus type 1 helicase/primase complex. Journal of virology 36 15994803
2021 Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling. Science advances 35 34433556
2017 Perturbation of RNA Polymerase I transcription machinery by ablation of HEATR1 triggers the RPL5/RPL11-MDM2-p53 ribosome biogenesis stress checkpoint pathway in human cells. Cell cycle (Georgetown, Tex.) 35 29143558
2011 Hydrophilic residues are crucial for ribosomal protein L11 (RPL11) interaction with zinc finger domain of MDM2 and p53 protein activation. The Journal of biological chemistry 31 21903592
2013 PICT1 regulates TP53 via RPL11 and is involved in gastric cancer progression. British journal of cancer 30 24045667
1997 Deletion mutants of the herpes simplex virus type 1 UL8 protein: effect on DNA synthesis and ability to interact with and influence the intracellular localization of the UL5 and UL52 proteins. Virology 29 9344911
2014 Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway. Carcinogenesis 28 25344835
2015 Down-regulation of 5S rRNA by miR-150 and miR-383 enhances c-Myc-rpL11 interaction and inhibits proliferation of esophageal squamous carcinoma cells. FEBS letters 27 26606907
2014 mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels. Cancer biology & therapy 27 25482947
2009 A mutation in helicase motif IV of herpes simplex virus type 1 UL5 that results in reduced growth in vitro and lower virulence in a murine infection model is related to the predicted helicase structure. The Journal of general virology 27 19403757
2013 Pediococcus acidilactici UL5 and Lactococcus lactis ATCC 11454 are able to survive and express their bacteriocin genes under simulated gastrointestinal conditions. Journal of applied microbiology 25 24279824
1994 Neuron-specific restriction of a herpes simplex virus recombinant maps to the UL5 gene. Journal of virology 25 8189514
2008 Mutations close to functional motif IV in HSV-1 UL5 helicase that confer resistance to HSV helicase-primase inhibitors, variously affect virus growth rate and pathogenicity. Antiviral research 24 18539344
1994 Identification of the pseudorabies virus UL4 and UL5 (helicase) genes. Virology 24 8030256
2018 Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23 30024791
2013 Assessment of hematopoietic failure due to Rpl11 deficiency in a zebrafish model of Diamond-Blackfan anemia by deep sequencing. BMC genomics 23 24341334
2014 Characterizing the molecular basis of attenuation of Marek's disease virus via in vitro serial passage identifies de novo mutations in the helicase-primase subunit gene UL5 and other candidates associated with reduced virulence. Journal of virology 22 24648463
2023 RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy. BMC molecular and cell biology 21 36869281
2022 MicroRNA-101-3p Suppresses Cancer Cell Growth by Inhibiting the USP47-Induced Deubiquitination of RPL11. Cancers 21 35205710
2001 The UL5 and UL52 subunits of the herpes simplex virus type 1 helicase-primase subcomplex exhibit a complex interdependence for DNA binding. The Journal of biological chemistry 20 11278618
1996 Pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of Pediococcus acidilactici UL5. The Journal of applied bacteriology 20 8698665
1975 RPL11, an R factor of Pseudomonas aeruginosa determining carbenicillin and gentamicin resistance. Antimicrobial agents and chemotherapy 20 806259
2005 The ribosomal protein rpL11 associates with and inhibits the transcriptional activity of peroxisome proliferator-activated receptor-alpha. Toxicological sciences : an official journal of the Society of Toxicology 18 16280383
2025 CircAKT3 promotes prostate cancer proliferation and metastasis by enhancing the binding of RPS27A and RPL11. Molecular cancer 16 39994725
2014 Clinical phenotype and genetic analysis of RPS19, RPL5, and RPL11 genes in Greek patients with Diamond Blackfan Anemia. Pediatric blood & cancer 15 25132370
2010 Effects of therapy using a helicase-primase inhibitor (HPI) in mice infected with deliberate mixtures of wild-type HSV-1 and an HPI-resistant UL5 mutant. Antiviral research 15 20420855
2019 UL23, UL30, and UL5 characterization of HSV1 clinical strains isolated from hematology department patients. Antiviral research 14 31152759
2017 Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in vivo and in vitro models. Biochemical and biophysical research communications 14 29225165
2019 RNAi mediated knockdown of RpL11, RpS2, and tra-2 led to reduced reproduction of Phytoseiulus persimilis. Experimental & applied acarology 13 31375950
2016 PICT-1 is a key nucleolar sensor in DNA damage response signaling that regulates apoptosis through the RPL11-MDM2-p53 pathway. Oncotarget 13 27829214
2022 Olaparib Induces RPL5/RPL11-Dependent p53 Activation via Nucleolar Stress. Frontiers in oncology 12 35719981
2022 Ribosomal protein RPL11 haploinsufficiency causes anemia in mice via activation of the RP-MDM2-p53 pathway. The Journal of biological chemistry 12 36435197
2015 Adult-onset Diamond-Blackfan anemia with a novel mutation in the exon 5 of RPL11: too late and too rare. Clinical case reports 12 26185635
2006 Genetic complexity of EHV-1 defective interfering particles and identification of novel IR4/UL5 hybrid proteins produced during persistent infection. Virus genes 12 16732484
2023 RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5. Proceedings of the National Academy of Sciences of the United States of America 11 38032932
2022 Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. International journal of oncology 11 35179222
2021 Single-cell analysis of erythropoiesis in Rpl11 haploinsufficient mice reveals insight into the pathogenesis of Diamond-Blackfan anemia. Experimental hematology 11 33631277
1982 [Interaction of Pseudomonas aeruginosa plasmids and mu-like phages: the suppression of the early stages of cell infection by phage D3112 in the presence of plasmid RPL11]. Genetika 10 6807753
2016 High conservation of herpes simplex virus UL5/UL52 helicase-primase complex in the era of new antiviral therapies. Antiviral research 8 26826441
2022 Identification of a small-molecule RPL11 mimetic that inhibits tumor growth by targeting MDM2-p53 pathway. Molecular medicine (Cambridge, Mass.) 7 36071402
2021 Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis via RPL11-MDM2-p53 Pathway in Mouse Spermatogonia. Biomedical and environmental sciences : BES 7 34782045
2024 SRBD1 Regulates the Cell Cycle, Apoptosis, and M2 Macrophage Polarization via the RPL11-MDM2-p53 Pathway in Glioma. Environmental toxicology 6 39258423
2022 Adult-Onset Diamond-Blackfan Anemia with RPL11 Gene Variation Case Report. The American journal of case reports 6 35038319
2021 Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells. International journal of molecular sciences 6 34948282
2008 Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene. Virus research 6 18582977
2014 Transcriptome analysis of Rpl11-deficient zebrafish model of Diamond-Blackfan Anemia. Genomics data 5 26484089
2013 V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. Oncogene 5 23563180
2025 SNORA47 affects stemness and chemotherapy sensitivity via EBF3/RPL11/c-Myc axis in luminal A breast cancer. Molecular medicine (Cambridge, Mass.) 4 40264043
2018 Detection of Residual Proteins UL5 and UL29 Using a Targeted Proteomics Approach in HSV529, a Replication-Deficient HSV-2 Vaccine Candidate. Journal of pharmaceutical sciences 4 30176254
2017 Involvement of RPL11 in the enhancement of P53 stability by a podophyllum derivative, a topoisomerase II inhibitor. Cell biology international 4 28949066
2015 The Mut UL5-I682R Marek's Disease Virus with a Single Nucleotide Mutation Within the Helicase-Primase Subunit Gene not only Reduces Virulence but also Provides Partial Vaccinal Protection Against Marek's Disease. Avian diseases 4 26292541
2013 Determination of HSV-1 UL5 and UL29 gene copy numbers in an HSV complementing Vero cell line. Journal of biotechnology 4 24140636
2022 The role of PICT1 in RPL11/Mdm2/p53 pathway-regulated inhibition of cell growth induced by topoisomerase IIα inhibitor against cervical cancer cell line. Biochemical pharmacology 3 35605655
2015 Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus. Acta biochimica Polonica 3 25730208
2015 Addition of a UL5 helicase-primase subunit point mutation eliminates bursal-thymic atrophy of Marek's disease virus ∆Meq recombinant virus but reduces vaccinal protection. Avian pathology : journal of the W.V.P.A 3 25968878
2000 Characterisation of IE and UL5 gene products of equine herpesvirus 1 using DNA inoculation of mice. Archives of virology 3 11205113
2015 Characterizing in vivo stability and potential interactions of a UL5 helicase-primase mutation previously shown to reduce virulence and in vivo replication of Marek's disease virus. Virus research 1 25828576
2009 One-step column purification of herpes simplex virus 1 helicase-primase subcomplex using C-terminally his-tagged UL5 subunit. Virus genes 1 19396588
2025 Intrafamilial variability in Diamond-Blackfan anemia with a novel canonical splice-site variant in the RPL11 gene. Laboratory medicine 0 40720759
2025 Silencing RPL11 attenuates acute kidney injury by suppressing tubular apoptosis and macrophage-driven inflammation. Frontiers in immunology 0 40895534
2025 Transcriptomic profiling of diabetic retinopathy: insights into RPL11 and bisphenol A. Frontiers in endocrinology 0 41384025
2023 Evidence of a Protein-Coding Gene Antisense to the UL5 Gene in Bovine Herpesvirus I. Viruses 0 37896756
2016 GENES CODING RIBOSOMAL PROTEIN RPL11 AND 5,8S RIBOSOMAL RNA IN SPECIES OF GENUS CHIRONOMUS: NUCLEOTIDE VARIABILITY, DIVERGENCE AND CHROMOSOMAL LOCATION. Tsitologiia 0 30188642

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