Affinage

EIF3B

Eukaryotic translation initiation factor 3 subunit B · UniProt P55884

Length
814 aa
Mass
92.5 kDa
Annotated
2026-06-09
54 papers in source corpus 29 papers cited in narrative 29 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EIF3B (yeast PRT1) is a core scaffolding subunit of the multi-subunit eIF3 translation initiation complex and serves as the nucleation core for assembly of the entire complex in vivo, with other subunits assembling around eIF3b and eIF3a in a defined hierarchical order (PMID:27924037, PMID:7798228, PMID:9388245). Architecturally, EIF3B is a modular protein: its non-canonical N-terminal RRM domain adopts a fold whose negatively charged beta-sheet surface is incompatible with RNA binding and instead uses its rear alpha-helices and a hydrophobic pocket to clamp a conserved tryptophan in the eIF3j N-terminal acidic motif, while simultaneously contacting the eIF3a (TIF32) CTD via the RNP1 motif (PMID:17190833, PMID:20060839, PMID:8995410, PMID:16581774). This RRM-mediated eIF3b–eIF3j–eIF3a-CTD module is positioned near the mRNA entry channel of the 40S subunit, where it governs preinitiation complex assembly, scanning, and AUG start codon selection — RRM removal or RNP1/pocket mutations dissociate partner subunits, abolish 40S binding, and increase leaky scanning (PMID:11179233, PMID:16581774, PMID:20584985, PMID:20060839). Its C-terminal WD40 nine-bladed beta-propeller directly contacts the 40S subunit and ribosomal protein rpS9e, anchoring eIF3 to the small subunit (PMID:24768115). Functionally, the complex promotes 80S dissociation, stabilizes Met-tRNAi binding to the 40S subunit, and is required for mRNA binding and functional 48S initiation complex formation (PMID:7798228, PMID:9388245). EIF3B serves as a docking platform for trans-acting RNA-binding factors that direct selective translation: P311 binds the non-canonical RRM to stimulate TGF-β translation (PMID:25336651), and EIF3B is recruited by viral IRES elements (coxsackievirus B3 domain V) (PMID:24063684). In cancer cells, EIF3B depletion blocks the G1–S transition by lowering protein (not mRNA) levels of cyclins and integrin α5 (PMID:23575475). Beyond canonical initiation, EIF3B also stabilizes specific proteins by antagonizing their E3-ligase-mediated ubiquitination — PTGS2 (against MDM2), PCNA (against SYVN1), and MAP2K2 (against VHL at K169) — and is itself stabilized by PUS1 (non-enzymatically), ADAM12, and METTL3 against proteasomal degradation (PMID:36050601, PMID:38687509, PMID:40691141, PMID:39247811, PMID:42117088, PMID:40576144).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1994 High

    Established that the PRT1/eIF3b ortholog is an integral subunit of a large multi-subunit eIF3 complex with defined initiation activities, fixing its place in translation initiation.

    Evidence Biochemical purification of yeast eIF3 and methionyl-puromycin synthesis assays

    PMID:7798228

    Open questions at the time
    • Did not assign a molecular function to PRT1/eIF3b itself within the complex
    • RNA-binding activity attributed to a different (62 kDa) subunit
  2. 1995 High

    Demonstrated PRT1 is functionally required for active translation and that its N-terminal region is needed for proper incorporation into the complex, revealing an assembly/scaffolding role.

    Evidence Cell-free translation complementation of ts prt1 mutants and dominant-negative N-terminal deletion analysis in yeast

    PMID:7623843 PMID:7876188

    Open questions at the time
    • Did not define which subunit interfaces the N-terminus mediates
    • No structural basis for assembly
  3. 1997 Medium

    Identified the human ortholog as a core eIF3 subunit and mapped its RRM domain as the region mediating direct contact with eIF3a, beginning the molecular dissection of eIF3b interactions.

    Evidence cDNA cloning, far Western direct binding, domain deletion mapping, and reconstituted 48S initiation assays in mammalian systems

    PMID:8995410 PMID:9388245

    Open questions at the time
    • RRM fold and binding mode not yet structurally resolved
    • Single-lab far Western for eIF3a interaction
  4. 2001 High

    Showed the eIF3b RRM simultaneously bridges eIF3j and eIF3a and is essential for holding the complex together and binding the 40S subunit, establishing eIF3b as a central scaffolding hub.

    Evidence In vivo reciprocal Co-IP, genetic suppressor analysis, and ribosome-binding assays in yeast; plus mapping of a separate eIF3e/Int-6 binding segment

    PMID:11179233 PMID:11457827

    Open questions at the time
    • Atomic structure of the RRM interfaces not yet determined
    • Functional consequence for AUG selection not yet measured
  5. 2006 High

    Resolved the structural basis for eIF3b's non-canonical RRM and the RNP1 motif's dual role, linking the eIF3b–eIF3j–eIF3a module mechanistically to 40S recruitment and start codon fidelity.

    Evidence NMR structure of human eIF3b-RRM with eIF3j binding mapping, plus yeast RNP1 mutagenesis with preinitiation complex isolation and leaky-scanning reporters

    PMID:16581774 PMID:17190833

    Open questions at the time
    • Tryptophan-pocket recognition detail not yet resolved
    • Position relative to mRNA entry channel inferred
  6. 2010 High

    Defined the precise tryptophan-in-pocket recognition between eIF3b-RRM and eIF3j and placed the module at the mRNA entry channel, explaining how it persists on scanning complexes to control AUG selection.

    Evidence NMR and crystal structures (human and yeast eIF3b-RRM), ITC, genetic epistasis, pocket-residue mutagenesis, ribosomal protein binding, and translation reporters

    PMID:20060839 PMID:20584985 PMID:20862284

    Open questions at the time
    • Divergent RNA-binding capacity of yeast vs human RRM left functionally unresolved
    • Dynamics during scanning not directly visualized
  7. 2014 High

    Determined the eIF3b WD40 beta-propeller structure and demonstrated direct contact with the 40S subunit via rpS9e, completing the picture of how eIF3b is anchored to the ribosome.

    Evidence X-ray crystallography of Chaetomium eIF3b WD40, cryo-EM map reinterpretation, and in vitro binding to 40S and isolated rpS9e

    PMID:24768115

    Open questions at the time
    • Full-length eIF3b within the complete eIF3–40S complex not crystallized
    • Coordination between RRM and WD40 contacts not defined
  8. 2016 High

    Established that human eIF3b is the obligate nucleation core for assembly of the entire 12-subunit eIF3 complex in vivo, elevating it from a scaffolding subunit to the assembly seed.

    Evidence Systematic RNAi of all 12 eIF3 subunits in human cells with reciprocal IP of complex composition

    PMID:27924037

    Open questions at the time
    • Order of co-assembly with chaperones not defined
    • No structural snapshot of the assembly intermediate
  9. 2014 High

    Showed the non-canonical RRM also serves as a docking site for trans-acting RNA-binding factors that direct selective mRNA translation, expanding eIF3b's role beyond general initiation.

    Evidence GST pulldown, SPR (Kd ~1.26 µM), domain mapping, luciferase reporters and polysome fractionation for P311-driven TGF-β translation

    PMID:25336651

    Open questions at the time
    • Whether other factors compete for the same RRM surface unknown
    • Generality across mRNA targets not established
  10. 2013 Medium

    Demonstrated that eIF3b loss in cancer cells blocks the G1–S transition and disrupts adhesion by lowering protein levels of cell-cycle regulators and integrin α5 without changing their mRNA, tying eIF3b to selective translational control of proliferation.

    Evidence siRNA knockdown, cell cycle analysis, protein-vs-RNA Western comparison, cytoskeleton staining, and integrin α5 phenocopy in human cancer cells

    PMID:23575475

    Open questions at the time
    • Direct mRNA targets not mapped
    • Single-lab phenotype
  11. 2018 Low

    Began cataloging RNA species that physically associate with EIF3B to direct context-specific translation, including IRES, lncRNA, and small RNA partners.

    Evidence RNA pulldown/LC-MS and RIP across viral IRES, piR-823, RP11-284P20.2/c-met, and TEX9 contexts

    PMID:24063684 PMID:30556540 PMID:31481019 PMID:32100822

    Open questions at the time
    • Several associations rest on single pulldown/RIP without polysome confirmation of selective translation
    • Mechanism of recruitment to each target inferred, not demonstrated
  12. 2022 Medium

    Revealed a non-canonical role in which EIF3B stabilizes specific oncoproteins by antagonizing their E3-ligase-mediated ubiquitination, distinct from its translation initiation function.

    Evidence Co-IP, ubiquitination assays, domain/site mapping, and rescue experiments for PTGS2/MDM2, PCNA/SYVN1, and MAP2K2/VHL-K169 in cancer cells

    PMID:36050601 PMID:38687509 PMID:40691141

    Open questions at the time
    • Whether stabilization is direct shielding or via competing complex formation unresolved
    • Each axis from a single lab/tumor context
  13. 2026 Medium

    Identified upstream regulators that stabilize EIF3B against proteasomal degradation and downstream signaling/metabolic programs it drives, defining EIF3B as a regulated node in oncogenic networks.

    Evidence Ubiquitination assays, cycloheximide chase, ChIP, Co-IP and functional rescue for PUS1, ADAM12, and METTL3 regulation and downstream EGFR/AKT and glycolysis effects

    PMID:39247811 PMID:40576144 PMID:42117088

    Open questions at the time
    • Whether stabilization couples to altered eIF3 complex activity unknown
    • Single-lab, individual cancer contexts

Open questions

Synthesis pass · forward-looking unresolved questions
  • How EIF3B's two distinct activities — ribosomal initiation scaffolding and ubiquitin-independent protein/mRNA stabilization — are coordinated, and whether selective mRNA binding and protein stabilization occur within or outside the eIF3 complex, remains unresolved.
  • No structure of full-length eIF3b in the assembled eIF3–40S complex
  • Selective translation claims largely rest on RIP/pulldown without ribosome occupancy data
  • Mechanistic link between scaffolding and protein-stabilization roles undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0045182 translation regulator activity 5 GO:0060090 molecular adaptor activity 4 GO:0005198 structural molecule activity 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005840 ribosome 3 GO:0005829 cytosol 2
Pathway
R-HSA-8953854 Metabolism of RNA 4 R-HSA-392499 Metabolism of proteins 3
Partners
ADAM12EIF3AEIF3EEIF3JMETTL3P311 (NREP)PUS1RPS9
Complex memberships
eIF3

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 Purified yeast eIF3 is a ~550 kDa complex of eight subunits; the 90-kDa subunit corresponds to PRT1 (EIF3B ortholog). The complex promotes dissociation of 80S ribosomes into subunits, stabilizes Met-tRNAi binding to 40S ribosomal subunits, and is required for mRNA binding. The 62-kDa subunit (not PRT1/EIF3B itself) was identified as the RNA-binding subunit within the complex. Biochemical purification from ribosomal salt wash, molecular sieve and ion exchange chromatography, methionyl-puromycin synthesis assay, immunoblotting The Journal of biological chemistry High 7798228
1995 Prt1 (EIF3B ortholog) co-purifies with four other polypeptides (130, 80, 75, 40, 32 kDa) as a protein complex that restores translation in a cell-free system derived from temperature-sensitive prt1 mutant yeast, demonstrating the complex is an active translation factor. An N-terminal in-frame deletion generates a dominant-negative form that competes with wild-type Prt1 for incorporation into the complex and inhibits 40S ribosome binding. Multi-step protein purification, cell-free translation complementation assay, subcellular fractionation, dominant-negative deletion analysis The Journal of biological chemistry High 7623843 7876188
1995 Mutational analysis of yeast Prt1 (EIF3B ortholog) identified six independent temperature-sensitive missense mutations distributed throughout the central and C-terminal regions. An N-terminal in-frame deletion creates a dominant-negative form that inhibits 40S ribosome association of wild-type Prt1, indicating the N-terminal region is required for proper complex assembly. Temperature-sensitive mutant characterization, subcellular fractionation, dominant-negative mutant analysis Molecular and cellular biology Medium 7623843
1997 Human PRT1 (EIF3B) is an integral subunit of human eIF3, migrating at 116 kDa. Far Western analysis shows that hPrt1 directly interacts with the p170 (eIF3a) subunit of eIF3. Mapping studies identify the RNA recognition motif (RRM) domain of hPrt1 as the region required for association with p170/eIF3a. cDNA cloning, immunoblotting with anti-eIF3 antibody, far Western analysis, domain deletion mapping The Journal of biological chemistry Medium 8995410
1997 The p110 subunit of mammalian eIF3, purified from rabbit reticulocyte lysates, was identified as the mammalian homologue of yeast Prt1p (EIF3B). The purified eIF3 complex (lacking p170) stimulated Met-tRNAf binding to 40S subunits, formed a functional 40S initiation complex at AUG, and was released from the 40S subunit during eIF5-dependent 60S subunit joining. Biochemical purification, cell-free initiation complex assembly assay, cDNA cloning and sequencing, immunochemical characterization The Journal of biological chemistry High 9388245
2001 The RRM domain of yeast eIF3b/PRT1 interacts simultaneously with HCR1 (eIF3j ortholog) and with an internal domain of TIF32 (eIF3a ortholog). Removal of the PRT1 RRM in vivo caused dissociation of TIF32, NIP1, HCR1, and eIF5 from eIF3, and destroyed 40S ribosome binding by the residual PRT1-TIF34-TIF35 subcomplex. Genetic suppressor analysis linked PRT1, HCR1, and TIF32 functionally. In vivo co-immunoprecipitation, genetic suppressor analysis, deletion mutant analysis, ribosome binding assays The EMBO journal High 11179233
2001 Yeast Pci8p and human eIF3e/Int-6 both interact with the eIF3b/Prt1 subunit by binding to a discrete segment of Prt1p in vivo and in vitro. Human eIF3e/Int-6 interacts with the homologous segment of human eIF3b. This defines a specific interaction domain on eIF3b distinct from its RRM. In vivo and in vitro binding assays, domain mapping, two-hybrid analysis The Journal of biological chemistry Medium 11457827
2006 The solution structure of the N-terminal RRM domain of human eIF3b was determined by NMR. The RRM has a non-canonical fold with a negatively charged beta-sheet surface incompatible with RNA binding. Instead, eIF3j binds to the rear alpha-helices of eIF3b-RRM (opposite the beta-sheet). The N-terminal 69-amino acid peptide of eIF3j is sufficient for binding eIF3b-RRM, and this interaction is essential for eIF3b-RRM recruitment to the 40S ribosomal subunit. NMR structure determination, in vitro binding assays, 40S ribosome binding experiments The Journal of biological chemistry High 17190833
2006 Mutating the RNP1 motif of yeast eIF3b/PRT1 (prt1-rnp1) impairs direct in vitro interactions with both eIF3a/TIF32 and eIF3j/HCR1, reduces 40S binding of eIF3 to native preinitiation complexes in vivo, reduces 40S-bound eIF5 and eIF1, and increases leaky scanning at GCN4 uORF1, indicating the PRT1 RNP1 motif is required for optimal preinitiation complex assembly and AUG recognition. Site-directed mutagenesis, in vitro pull-down, native preinitiation complex isolation, polysome/ribosome analysis, genetic reporter assay for leaky scanning Molecular and cellular biology High 16581774
2010 The eIF3b/PRT1 RRM and eIF3j/HCR1 N-terminal domain (NTD) both interact with the CTD of eIF3a/TIF32. Mutations in eIF3a and eIF3j that disrupt their interactions with the eIF3b RRM increase leaky scanning at an AUG codon. The extreme CTD of eIF3a/TIF32 binds ribosomal proteins Rps2 and Rps3, placing the eIF3b-RRM-eIF3j-eIF3a-CTD module near the mRNA entry channel. Genetic epistasis, in vitro pull-down, mutant phenotype analysis, ribosomal protein binding assays Molecular and cellular biology Medium 20584985
2010 NMR structure of the human eIF3b-RRM/eIF3j NTA interaction: a conserved tryptophan in the eIF3j N-terminal acidic motif (NTA) is held in the helix alpha1/loop 5 hydrophobic pocket of eIF3b-RRM. Mutating corresponding 'pocket' residues in yeast eIF3b/PRT1 eliminates eIF3j/HCR1 association in vitro and in vivo, reduces 40S occupancy of eIF3, and produces leaky scanning defects suppressible by overexpressed eIF1A, indicating eIF3j remains on scanning preinitiation complexes and cooperates with eIF3b-RRM for AUG selection. NMR structure determination, in vitro and in vivo binding assays, 40S ribosome co-sedimentation, genetic suppressor analysis (eIF1A overexpression), translation reporter assays Journal of molecular biology High 20060839
2010 Crystal structure of yeast eIF3b-RRM shows the same fold as human eIF3b-RRM with similar surface charge at the eIF3j-binding interface. Thermodynamic analysis confirms the same range of enthalpy change and dissociation constant for yeast and human eIF3b-RRM/eIF3j interactions. Unlike human eIF3b-RRM, the yeast RRM beta-sheet surface is compatible with RNA binding, and the yeast domain was confirmed to interact with yeast total RNA. X-ray crystallography, ITC thermodynamic analysis, RNA binding assay with yeast total RNA PloS one High 20862284
2014 Crystal structure of the WD40 domain of Chaetomium thermophilum eIF3b reveals a nine-bladed beta-propeller fold, conserved across all eIF3b orthologs. In vitro binding assays demonstrate that eIF3b directly binds 40S ribosomes and isolated ribosomal protein rpS9e, consistent with placement of eIF3b near the 40S subunit as suggested by cryo-EM reinterpretation. X-ray crystallography, cryo-EM map analysis, in vitro ribosome binding assay, direct binding to isolated rpS9e Structure High 24768115
2014 P311 (an intrinsically disordered RNA-binding protein) directly interacts with the non-canonical RRM of eIF3b (Kd ~1.26 μM), as shown by GST pulldown and surface plasmon resonance. This P311-eIF3b interaction is required for stimulation of TGF-β1, -2, and -3 translation; disruption of the P311-eIF3b binding inhibited TGF-β translation as shown by luciferase reporter assay and polysome fractionation. Co-immunoprecipitation/mass spectrometry, GST pulldown, surface plasmon resonance, domain mapping, luciferase reporter assay, polysome fractionation, RNA-protein EMSA The Journal of biological chemistry High 25336651
2016 Human eIF3b serves as the nucleation core for assembly of the entire eIF3 complex: in the absence of eIF3b knockdown (RNAi), neither the yeast-like core module nor the octamer module forms in vivo. Other subunits assemble around eIF3b and eIF3a in a defined hierarchical order. eIF3d knockdown causes proliferation defects without disrupting eIF3 integrity. RNAi knockdown of each of 12 eIF3 subunits in human cells, immunoprecipitation of eIF3 complex, Western blotting of subunit composition Nucleic acids research High 27924037
2013 eIF3b depletion by siRNA in human cancer cell lines inhibited G1-S cell cycle transition by changing protein (but not RNA) expression of cyclin A, E, Rb, and p27Kip1, disrupted actin cytoskeleton and focal adhesions, and decreased integrin α5 protein expression. Integrin α5 depletion phenocopied eIF3b depletion effects. siRNA knockdown, cell cycle analysis, Western blotting (protein vs. RNA level comparison), actin/focal adhesion staining, integrin α5 knockdown phenocopy Clinical cancer research Medium 23575475
2013 eIF3b (p116) directly binds to domain V of the coxsackievirus B3 CVB3 IRES, as identified by proteomic pulldown. A single Sabin3-like point mutation (U473→C) in domain V impairs the binding affinity of eIF3b to the IRES domain, providing a mechanism for translation attenuation of the attenuated viral strain. RNA pulldown with domain V RNA, proteomics identification, binding affinity comparison between wild-type and mutant IRES Diagnostic pathology Medium 24063684
2018 piR-823 physically associates with EIF3B protein (identified by RNA pulldown and LC-MS), and the piR-823/EIF3B combination promotes TGF-β1 expression in hepatic stellate cells to drive their activation. RNA pulldown with LC-MS, overexpression/inhibition experiments, TGF-β1 protein measurement Medical science monitor Low 30556540
2019 eIF3b binds to TEX9 mRNA, as confirmed by RNA immunoprecipitation. eIF3b and TEX9 synergistically promote proliferation and migration and activate AKT signaling in esophageal squamous cell carcinoma cells. Quantitative proteomics, RNA immunoprecipitation (RIP), functional cell assays, Western blot for AKT pathway BMC cancer Low 31481019
2020 RP11-284P20.2 lncRNA binds both c-met mRNA and EIF3b protein (confirmed by RNA immunoprecipitation and RNA pulldown), and likely recruits EIF3b to c-met mRNA to facilitate its translation, increasing c-met protein without affecting c-met mRNA levels. RNA immunoprecipitation, RNA pulldown, Western blot (protein vs. mRNA), RNA fluorescence in situ hybridization Bioscience reports Low 32100822
2022 EIF3B stabilizes PTGS2 protein by inhibiting PTGS2 ubiquitination mediated by the E3 ligase MDM2, thereby protecting PTGS2 from proteasomal degradation. PTGS2 overexpression rescued the anti-tumor effects of EIF3B silencing in melanoma cells. Co-immunoprecipitation, ubiquitination assay, Western blotting, rescue experiment with PTGS2 overexpression Cancer science Medium 36050601
2024 PUS1 (pseudouridine synthase 1) protects EIF3B from ubiquitin-mediated proteasomal degradation in a non-enzymatic manner. FOXA1 transcription factor drives PUS1 expression by binding its promoter, and EIF3B acts as a downstream effector of PUS1 to promote prostate cancer bone metastasis. RNAi knockdown, overexpression rescue experiments, ubiquitination assays, Western blotting, ChIP (FOXA1 promoter binding) International journal of biological sciences Medium 39247811
2024 EIF3B stabilizes PCNA protein by inhibiting PCNA ubiquitination mediated by the E3 ligase SYVN1. Knockdown of PCNA attenuated the cholangiocarcinoma-promoting effects of EIF3B overexpression, and elevated P21 protein in shEIF3B cells was partially reduced by a P21 signaling pathway inhibitor. Co-immunoprecipitation, ubiquitination assay, shRNA knockdown, overexpression and rescue experiments, Western blotting Aging Medium 38687509
2025 EIF3B forms a protein complex with METTL3, as confirmed by co-immunoprecipitation. EIF3B overexpression activates the EGFR/AKT signaling pathway in cervical cancer cells; this cancer-promoting effect is lost when METTL3 is silenced, indicating the EIF3B-METTL3 complex (not EIF3B alone) drives oncogenic signaling. EIF3B and METTL3 do not regulate each other's expression. Co-immunoprecipitation, siRNA/overexpression manipulation, cell proliferation/invasion assays, Western blot for EGFR/AKT pathway Oncology reports Medium 40576144
2025 EIF3B directly interacts with the P3 domain of MAP2K2 and inhibits VHL-mediated ubiquitination of MAP2K2 at K169, thereby stabilizing MAP2K2. MAP2K2 kinase activity is required for EIF3B-driven ERK phosphorylation and downstream oncogenic signaling in laryngeal squamous cell carcinoma. Co-immunoprecipitation, ubiquitination assay, domain mapping (P3 domain interaction), site-specific mutation (K169), Western blot (pERK), MAP2K2 knockdown rescue Cell death discovery Medium 40691141
2025 EIF3B specifically binds miR-100-5p in prostate cancer cells (confirmed by pull-down assay), and EIF3B knockdown decreases the enrichment of miR-100-5p in PC-3-derived exosomes, indicating EIF3B mediates selective sorting of miR-100-5p into exosomes. RNA pull-down assay, EIF3B knockdown, exosome miRNA quantification Scientific reports Low 40681642
2026 ADAM12 physically interacts with EIF3B (identified by immunoprecipitation-mass spectrometry and confirmed by co-IP). ADAM12 stabilizes EIF3B by limiting ubiquitin-proteasome-mediated degradation (cycloheximide chase showed accelerated EIF3B degradation upon ADAM12 knockdown; ubiquitination assay confirmed increased EIF3B ubiquitination). EIF3B downstream promotes PKM2/LDHA expression and glycolysis; EIF3B overexpression rescued metabolic and tumorigenic effects of ADAM12 knockdown. Immunoprecipitation-mass spectrometry, co-immunoprecipitation, cycloheximide chase, ubiquitination assay, metabolic assays (ECAR, OCR, lactate, glucose uptake), rescue overexpression Journal of hepatocellular carcinoma Medium 42117088
2025 miR-124-3p directly targets EIF3B mRNA (inverse correlation of expression confirmed), and miR-124-3p/EIF3B regulate apoptosis in Chlamydia psittaci-infected human bronchial epithelial cells via the PI3K/AKT signaling pathway. EIF3B siRNA reversed the anti-apoptotic effect of miR-124-3p inhibition. miRNA mimic/inhibitor experiments, EIF3B siRNA, flow cytometry (apoptosis), Western blot (PI3K/AKT), mRNA expression correlation The Journal of infectious diseases Low 39561162
2025 EIF3B binds TBK1 and PIK3CA mRNAs (confirmed by RNA immunoprecipitation) and regulates their translation, thereby activating TBK1, PI3K/AKT, and JAK2/STAT3 signaling pathways in KRAS-mutant colorectal adenocarcinoma cells. RNA immunoprecipitation (RIP), RNA sequencing, RT-qPCR, Western blot for pathway proteins, EIF3B siRNA knockdown Journal of biochemical and molecular toxicology Low 40503732

Source papers

Stage 0 corpus · 54 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding. The EMBO journal 96 11179233
1994 Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein. The Journal of biological chemistry 95 7798228
2010 The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Molecular and cellular biology 79 20584985
2010 The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection. Journal of molecular biology 76 20060839
2016 Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer. Nucleic acids research 74 27924037
2013 Translation initiation factor eIF3b expression in human cancer and its role in tumor growth and lung colonization. Clinical cancer research : an official journal of the American Association for Cancer Research 71 23575475
1997 The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. The Journal of biological chemistry 70 8995410
1987 Regulated arrest of cell proliferation mediated by yeast prt1 mutations. Experimental cell research 57 3308493
1995 Isolation of a protein complex containing translation initiation factor Prt1 from Saccharomyces cerevisiae. The Journal of biological chemistry 56 7876188
2006 Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit. The Journal of biological chemistry 55 17190833
2006 Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Molecular and cellular biology 52 16581774
1987 Molecular characterization of the yeast PRT1 gene in which mutations affect translation initiation and regulation of cell proliferation. The Journal of biological chemistry 51 3029094
2012 Knockdown of eukaryotic translation initiation factors 3B (EIF3B) inhibits proliferation and promotes apoptosis in glioblastoma cells. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 49 22234522
1997 Biochemical characterization of mammalian translation initiation factor 3 (eIF3). Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1. The Journal of biological chemistry 48 9388245
2018 The Combination of piR-823 and Eukaryotic Initiation Factor 3 B (EIF3B) Activates Hepatic Stellate Cells via Upregulating TGF-β1 in Liver Fibrogenesis. Medical science monitor : international medical journal of experimental and clinical research 44 30556540
2017 Silencing of translation initiation factor eIF3b promotes apoptosis in osteosarcoma cells. Bone & joint research 34 28360085
2001 Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits. The Journal of biological chemistry 34 11457827
2014 Translation initiation factor eIF3b contains a nine-bladed β-propeller and interacts with the 40S ribosomal subunit. Structure (London, England : 1993) 32 24768115
2014 Novel RNA-binding protein P311 binds eukaryotic translation initiation factor 3 subunit b (eIF3b) to promote translation of transforming growth factor β1-3 (TGF-β1-3). The Journal of biological chemistry 31 25336651
1995 Mutational analysis of the Prt1 protein subunit of yeast translation initiation factor 3. Molecular and cellular biology 31 7623843
1999 Cell surface protease PRT1 identified in the fungal pathogen Pneumocystis carinii. Molecular microbiology 27 10209745
2019 EIF3B is associated with poor outcomes in gastric cancer patients and promotes cancer progression via the PI3K/AKT/mTOR signaling pathway. Cancer management and research 25 31686906
2002 Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. Journal of bacteriology 25 11948174
2014 Characterization of an extensin-modifying metalloprotease: N-terminal processing and substrate cleavage pattern of Pectobacterium carotovorum Prt1. Applied microbiology and biotechnology 24 24946865
2004 Expression and complexity of the PRT1 multigene family of Pneumocystis carinii. Microbiology (Reading, England) 21 14766907
2002 Transcription of Xanthomonas campestris prt1 gene encoding protease 1 increases during stationary phase and requires global transcription factor Clp. Biochemical and biophysical research communications 20 12083764
1986 Isolation and characterization of PRT1, a gene required for the initiation of protein biosynthesis in Saccharomyces cerevisiae. Molecular and cellular biology 20 3025657
2018 Effects of EIF3B gene downregulation on apoptosis and proliferation of human ovarian cancer SKOV3 and HO-8910 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 18 30551537
2021 EIF3B promotes cancer progression in pancreatic cancer. Scandinavian journal of gastroenterology 13 33459066
2019 TEX9 and eIF3b functionally synergize to promote the progression of esophageal squamous cell carcinoma. BMC cancer 13 31481019
2010 Crystal structure of the RNA recognition motif of yeast translation initiation factor eIF3b reveals differences to human eIF3b. PloS one 13 20862284
2024 FOXA1-dependent PUS1 regulates EIF3b stability in a non-enzymatic pathway mediating prostate cancer bone metastasis. International journal of biological sciences 10 39247811
1987 Evolutionary relationships between laboratory mice and subspecies of Mus musculus based on the genetic study of pancreatic proteinase loci, Prt-1, Prt-2, Prt-3, and Prt-6. Biochemical genetics 10 3300637
2024 EIF3B affects the invasion and metastasis of hepatocellular carcinoma cells via the TGFBI/MAPK/ERK pathway. Annals of hepatology 8 39276983
2020 Long non-coding RNA RP11-284P20.2 promotes cell proliferation and invasion in hepatocellular carcinoma by recruiting EIF3b to induce c-met protein synthesis. Bioscience reports 7 32100822
2020 eIF3b regulates the cell proliferation and apoptosis processes in chronic myelogenous leukemia cell lines via regulating the expression of C3G. Biotechnology letters 7 32236758
2024 EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma. Aging 6 38687509
2022 EIF3B stabilizes PTGS2 expression by counteracting MDM2-mediated ubiquitination to promote the development and progression of malignant melanoma. Cancer science 5 36050601
2013 Impaired binding of standard initiation factors eIF3b, eIF4G and eIF4B to domain V of the live-attenuated coxsackievirus B3 Sabin3-like IRES--alternatives for 5'UTR-related cardiovirulence mechanisms. Diagnostic pathology 5 24063684
2025 MiR-124-3p/EIF3B Regulates Host Cell Apoptosis Induced by Chlamydia psittaci Through PI3K/AKT Signaling Pathway. The Journal of infectious diseases 4 39561162
2025 Shared molecular biomarkers and therapeutic targets in rheumatoid arthritis and osteoarthritis: Focus on EIF3B, KHSRP, NCL, PDCD1LG2, and SLC25A37. Cytokine 3 40592131
2025 Prostate cancer cell-derived exosomes inhibit macrophage phagocytosis through EIF3B-mediated exosomal sorting of miR-100-5p. Scientific reports 3 40681642
2026 CD133+-Derived Exosomes Carrying EIF3B Mediate Cell Metasta sis and Stemness in Colorectal Cancer. Current cancer drug targets 2 40660438
2001 Functional glycosylphosphatidylinositol anchor signal sequences in the Pneumocystis carinii PRT1 protease family. American journal of respiratory cell and molecular biology 2 11694452
2025 EIF3B Promotes KRAS Gene Mutation-Driven Colon Adenocarcinoma Progression Through the TBK1 and PI3K/AKT Signaling Pathways. Journal of biochemical and molecular toxicology 1 40503732
2025 EIF3B‑METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer. Oncology reports 1 40576144
2025 EIF3B stabilizes MAP2K2 to activate the ERK pathway and promote the progression of laryngeal squamous cell carcinoma. Cell death discovery 1 40691141
2025 [The study on effect of EIF3B in laryngeal carcinoma]. Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery 1 40744883
2025 eIF3b-driven autophagy and Wnt/β-catenin crosstalk: a novel regulatory axis in adriamycin resistance of breast cancer. Oncology reviews 1 41089706
2021 EIF3B Associates with Exacerbated Clinical Features, Poor Treatment Response and Survival in Adult Philadelphia Chromosome Negative Acute Lymphoblastic Leukemia Patients. Technology in cancer research & treatment 1 34617851
2026 EIF3B regulates the cell cycle of lung adenocarcinoma cells by activating the GTSE1 mediated ERK/MAPK pathway. Respiratory research 0 41668065
2026 Inhibition of eukaryotic translation initiation factor 1 A (eIF1A) and 3B (eIF3B) diminishes the psoriatic phenotype in two mouse models and human 3D model samples. Journal of dermatological science 0 41916779
2026 ADAM12 Stabilizes EIF3B to Promote Glycolysis and Tumor Progression in Hepatocellular Carcinoma. Journal of hepatocellular carcinoma 0 42117088
2025 Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin. Nature communications 0 40841552

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