| 2005 |
Fission yeast has two distinct eIF3 complexes sharing common core subunits but distinguished by the PCI proteins eIF3e and the novel eIF3m; eIF3m (unlike eIF3e) is an essential gene required for global cellular protein synthesis and polysome formation, and the eIF3m complex associates with the bulk of cellular mRNAs whereas the eIF3e complex associates with a far more restricted set. |
Genetic analysis (essential gene determination), polysome profiling, ribonomic/microarray approach, RT-PCR validation of 14 mRNAs |
BMC biology |
High |
15904532
|
| 2013 |
Drosophila Tango7 (ortholog of EIF3M) collaborates with the Drosophila apoptosome to drive caspase-dependent cellular remodeling required to resolve individual sperm from a syncytium; Tango7 is required for caspase activity, localizes to the active apoptosome compartment via its C terminus, and directly stimulates the activity of this complex in vitro. |
Genetic loss-of-function, in vitro apoptosome activity assay, C-terminal domain localization experiments |
Genes & development |
High |
23913920
|
| 2017 |
Drosophila Tango7 (ortholog of EIF3M) regulates cortical (but not cytoplasmic) activity of the initiator caspase Dronc in living salivary gland cells, enabling F-actin dismantling and tissue stretching; this is distinct from the canonical Dark (Apaf-1) adaptor that governs cytoplasmic Dronc activity during cell death, defining mutually exclusive subcellular domains of caspase activity. |
Genetic loss-of-function in Drosophila salivary glands, live imaging of F-actin dynamics, subcellular fractionation/localization studies |
Nature communications |
High |
28928435
|
| 2010 |
In human colon cancer cells, eIF3m silencing by siRNA affects cell proliferation, cell cycle progression, and cell death; a ribonomic approach identified that eIF3m influences the mRNA levels of MIF and MT2, and that eIF3m-dependent regulation of MT2A controls downstream CDC25A expression required for cell cycle progression. |
siRNA knockdown, ribonomics, RT-PCR, cell cycle analysis |
Oncogene |
Medium |
20838379
|
| 2019 |
In vivo RNAi-mediated knockdown of eIF3m in mouse liver leads to inhibition of rRNA processing, increased transcription of ribosomal protein genes, and metabolic gene expression changes, but yields few detectable differences in translation of particular mRNAs; a similar reduction in eIF3m protein is associated with induction of the mTOR pathway in vitro but not in vivo. |
RNAi knockdown in mouse liver, ribosome profiling, transcriptome sequencing, whole proteome and phosphoproteome analyses |
Molecular therapy. Nucleic acids |
High |
31855834
|
| 2021 |
EIF3m binds to the 5'UTR of CAPRIN1 mRNA and positively modulates its expression at the post-transcriptional level; additionally, EIF3m interacts with the deubiquitinase UCHL5, which stabilizes EIF3m protein and promotes its accumulation in lung adenocarcinoma cells. |
RNA immunoprecipitation (5'UTR binding), co-immunoprecipitation (UCHL5 interaction), gain- and loss-of-function assays |
American journal of cancer research |
Medium |
33791168
|
| 2026 |
USP34 maintains eIF3m protein stability through deubiquitination; the stabilized eIF3m binds directly to the 5'UTR of MTCH2 mRNA to upregulate MTCH2 expression, thereby maintaining mitochondrial function in triple-negative breast cancer cells. |
Co-immunoprecipitation, GST pull-down, RNA immunoprecipitation, RNA pull-down, JC-1 mitochondrial membrane potential assay, MitoSOX assay |
Journal of histotechnology |
Medium |
42023842
|
| 2026 |
EIF3m, especially its C-terminal part, interacts with FAdV-4 ORF1B protein and co-localizes with it in the cytoplasm; overexpression of eIF3m promotes FAdV-4 replication while knockdown reduces it, indicating the virus hijacks eIF3m to facilitate infection. |
Co-immunoprecipitation coupled with mass spectrometry, domain mapping, co-localization imaging, overexpression and knockdown assays |
Poultry science |
Medium |
41637784
|