| 2011 |
USP34 was identified in purified axin-containing protein complexes by LC-MS/MS and shown to stabilize axin by opposing its tankyrase-dependent ubiquitination, thereby functioning downstream of the β-catenin destruction complex to regulate Wnt/β-catenin signaling; RNAi knockdown of USP34 led to axin degradation and inhibition of β-catenin-mediated transcription. |
LC-MS/MS of purified axin complexes, RNA interference, Wnt/β-catenin reporter assays |
Molecular and cellular biology |
High |
21383061
|
| 2013 |
USP34 stabilizes the E3 ubiquitin ligase RNF168 by deubiquitylation in response to DNA double-strand breaks; loss of USP34 causes rapid RNF168 degradation, attenuated DSB-associated ubiquitylation, defective recruitment of BRCA1 and 53BP1, and compromised cell survival after ionizing radiation. |
siRNA knockdown, immunofluorescence for DSB repair factors (BRCA1, 53BP1), clonogenic survival assay, ubiquitylation assays |
Nucleic acids research |
High |
23863847
|
| 2013 |
In a genome-wide siRNA DUB screen in T lymphocytes, knockdown of USP34 selectively enhanced TCR-driven NF-κB activation, led to more pronounced degradation of the NF-κB inhibitor IκBα, and increased NF-κB DNA-binding activity, positioning USP34 as a negative regulator of NF-κB signaling downstream of TCR engagement. |
siRNA library screen, NF-κB reporter assay, IκBα degradation analysis, NF-κB DNA binding assay |
Cell communication and signaling : CCS |
Medium |
23590831
|
| 2018 |
USP34 stabilizes both Smad1 and RUNX2 by deubiquitylation in mesenchymal stem cells; USP34 depletion inhibits osteogenic differentiation and BMP2 signaling responses, and depletion of Smurf1 (an E3 ligase for Smad1) restores the osteogenic potential of Usp34-deficient MSCs in vitro, placing USP34 in opposition to Smurf1-mediated degradation of Smad1/RUNX2. |
Conditional knockout in mice, siRNA knockdown, ubiquitylation assays, epistasis (Smurf1 co-depletion rescue), bone phenotype analysis (micro-CT, histology) |
The EMBO journal |
High |
30181118
|
| 2018 |
USP34 was identified as an interaction partner of the E3 ligase gp78 by Co-IP/LC-MS/MS; USP34 knockdown facilitates proteasomal degradation of gp78 and consequently impairs gp78-mediated lipid droplet formation. |
Co-IP coupled to LC-MS/MS, siRNA knockdown, lipid droplet formation assay |
Biochemical and biophysical research communications |
Medium |
30585151
|
| 2024 |
USP34 deubiquitinates and stabilizes the peptidyl-prolyl isomerase Pin1; this interaction is facilitated by Plk1-mediated phosphorylation of Pin1, and stabilized Pin1 promotes isomerization of the SUMO E2 enzyme Ubc9 (requiring CDK1-mediated phosphorylation of Ubc9), leading to elevated SUMO1-modified protein hypersumoylation that supports glioma stem cell maintenance. |
Co-immunoprecipitation, ubiquitylation assays, pharmacological inhibition (Plk1 inhibitor, CDK1 inhibitor), in vitro isomerization assay, orthotopic tumor xenograft |
Nature communications |
High |
38167292
|
| 2020 |
USP34 interacts with SOX2, reduces its polyubiquitination, and stabilizes SOX2 protein (without affecting SOX2 mRNA), thereby promoting cell survival and cisplatin resistance in laryngeal squamous cell carcinoma cells. |
Co-immunoprecipitation, ubiquitylation assay, siRNA knockdown, overexpression rescue, cell viability assay |
The Kaohsiung journal of medical sciences |
Medium |
32783291
|
| 2021 |
USP34 stabilizes the transcription factor NFIC by deubiquitylation in dental pulp cells; conditional deletion of Usp34 in dental mesenchymal cells reduces NFIC levels and impairs odontogenic differentiation, resulting in short root anomaly, and overexpression of NFIC partially restores the differentiation defect. |
Conditional knockout in mice, siRNA knockdown, overexpression rescue, ubiquitylation assay, histomorphometry |
International journal of oral science |
High |
33686052
|
| 2022 |
In Drosophila, loss of Usp34 by siRNA abolishes homologous recombination at I-SceI-induced DSBs (DR-white assay), demonstrating an indispensable role for Usp34 specifically in homologous recombination-mediated DSB repair. |
siRNA knockdown, DR-white homologous recombination reporter assay, survival assay after UV/X-ray irradiation |
Scientific reports |
Medium |
35393473
|
| 2023 |
An siRNA screen of 96 DUBs in endothelial and HeLa cells identified USP34 as a regulator of thrombin-GPCR (PAR1)-driven p38 MAPK signaling; USP34 knockdown decreased thrombin-stimulated p38 phosphorylation and reduced IL-6 cytokine expression, but had no effect on endothelial barrier permeability. |
siRNA library screen, p38 phosphorylation assay, IL-6 ELISA, endothelial permeability assay |
The Journal of biological chemistry |
Medium |
37865315
|
| 2024 |
USP34 knockdown in endothelial cells increases PAR1 (F2R) mRNA transcript levels, leading to elevated PAR1 cell surface abundance and altered thrombin-stimulated p38 activation; this effect is not due to altered PAR1 ubiquitination, internalization, or degradation, but to transcriptional regulation of the F2R gene. |
siRNA knockdown, flow cytometry, RT-PCR, receptor internalization and degradation assays, p38 phosphorylation assay |
Molecular biology of the cell |
Medium |
39705380
|
| 2024 |
USP34 mediates BPTF-regulated stabilization of FOXC1 via deubiquitylation; immunoprecipitation experiments showed that BPTF affects FOXC1 protein stability through USP34-dependent de-ubiquitylation, and FOXC1 overexpression rescues the phenotype of BPTF knockdown in glioma cells. |
Co-immunoprecipitation, ubiquitylation assay, western blot, lentiviral overexpression/knockdown, functional rescue |
Histology and histopathology |
Medium |
38686761
|
| 2025 |
USP34 interacts with c-Myc, reduces its ubiquitination, and stabilizes the c-Myc protein; USP34 knockdown enhances c-Myc ubiquitination and degradation, and reduces aerobic glycolysis in hepatocellular carcinoma cells; overexpression of c-Myc reverses si-USP34-mediated phenotypic effects. |
Co-immunoprecipitation, ubiquitylation assay, siRNA knockdown, CCK-8, glycolysis assays, overexpression rescue |
The Turkish journal of gastroenterology |
Medium |
40260316
|
| 2026 |
USP34 deubiquitinates and stabilizes ANT1 (adenine nucleotide translocase 1) in TMJ chondrocytes; USP34 deficiency in chondrocyte-specific Usp34 KO mice leads to impaired ANT1-dependent initiation of PINK1-Parkin mitophagy and age-dependent TMJ osteoarthritis; USP34 overexpression protects chondrocytes against cellular injury. |
Chondrocyte-specific conditional KO mouse model, micro-CT/histomorphometry, Co-IP, ubiquitylation assay, mitophagy assays, overexpression studies |
JBMR plus |
High |
41631201
|
| 2026 |
USP34 stabilizes eIF3m protein through deubiquitylation in triple-negative breast cancer cells; stabilized eIF3m binds the 5'UTR of MTCH2 mRNA to upregulate MTCH2 expression, thereby maintaining mitochondrial function and promoting TNBC proliferation. |
Co-immunoprecipitation, GST pulldown, RNA immunoprecipitation, RNA pulldown, ubiquitylation assay, siRNA knockdown, mitochondrial function assays |
Journal of histotechnology |
Medium |
42023842
|