Affinage

RBBP7

Histone-binding protein RBBP7 · UniProt Q16576

Length
425 aa
Mass
47.8 kDa
Annotated
2026-06-10
39 papers in source corpus 25 papers cited in narrative 25 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBBP7 (RbAp46) is a WD40-repeat histone chaperone and chromatin adaptor that serves as a shared subunit of histone-modifying machinery and links chromatin regulation to cell-cycle control, chromosome segregation, and gene expression (PMID:24317350, PMID:28179136). Through its WD40 domains it binds histone H4, and this histone-binding function — abolished by a truncating mutation in the sixth WD40 domain — is essential for spermatogenesis, with male infertility rescued by wild-type but not mutant human RBBP7 (PMID:37843278). As part of histone deacetylase machinery it drives maturation-associated histone deacetylation required for chromosomal passenger complex localization and faithful meiotic chromosome segregation in oocytes (PMID:24317350), while RBBP7 together with its paralog supports H3.3 deposition and chromatin regulation during preimplantation development (PMID:34709113). RBBP7 acts as the substrate-receptor (DCAF) subunit of CRL4 ubiquitin ligase complexes: as CRL4(RBBP7) it stabilizes soluble CENP-A and mediates its centromeric loading (PMID:25795299), and as CRL4B it bridges and degrades HUWE1, thereby controlling MCL-1 and BRCA1 levels (PMID:29738775); orthologs are likewise required for CENP-A deposition at centromeres (PMID:26904949, PMID:33872374). Beyond chromatin, RBBP7 is phosphorylated by AMPK, which enhances its interaction with DNMT1 to inhibit DNA methylation and remodel nucleosomes (PMID:28143904), recruits LSD1 to erase repressive H3K9me3 at stemness gene promoters (PMID:40038738), and directly deacetylates the non-histone substrate Acsl4 at K401 to modulate its enzyme activity and ferroptosis (PMID:41478474). RBBP7 also functions in transcriptional regulation through interactions with BRCA1-BRCT (PMID:11394910) and HDAC1/Sp1 at target promoters (PMID:25885317), and its expression-level changes modulate growth, apoptosis, and JNK signaling (PMID:9765217, PMID:15456884).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1998 Medium

    Established RBBP7 as a growth-regulatory gene downstream of a tumor suppressor, providing the first functional context for the protein.

    Evidence Suppression subtractive hybridization identifying RbAp46 as a WT1 target, with colony formation and growth assays

    PMID:9765217

    Open questions at the time
    • Did not define the molecular mechanism of growth inhibition
    • WT1-dependence shown but RBBP7 biochemical activity not addressed
  2. 2001 Medium

    Connected RBBP7 to transcriptional regulation and DNA-damage signaling by mapping a WD-repeat-dependent interaction with the BRCA1 BRCT domain that represses p21 transactivation.

    Evidence Yeast two-hybrid, co-IP, luciferase reporter and deletion mapping

    PMID:11394910

    Open questions at the time
    • Functional consequence at endogenous BRCA1 targets genome-wide unknown
    • Mechanism of disruption by DNA damage not resolved
  3. 2003 Medium

    Linked RBBP7 overexpression to apoptotic and tumor-suppressive output via the JNK/GADD45 pathway, placing it upstream of a stress-signaling cascade.

    Evidence Inducible expression, JNK activity assays, dominant-negative JNK1 epistasis, xenografts (idx 2,3,4)

    PMID:11433396 PMID:12767060 PMID:14981905

    Open questions at the time
    • How a chromatin adaptor activates JNK is not mechanistically defined
    • Overexpression phenotypes may not reflect endogenous function
  4. 2004 High

    Used the Drosophila ortholog p55 to place RBBP7 in pRB/E2F transcriptional repression, distinguishing cell-cycle-independent from proliferation-coupled repression modes.

    Evidence RNAi depletion of p55 with E2F target RT-PCR/reporter assays and RBF complex epistasis

    PMID:15456884

    Open questions at the time
    • Direct mammalian validation not performed in this study
    • Which complexes mediate each repression mode not delineated
  5. 2015 Medium

    Defined RBBP7 as a substrate-specific adaptor (DCAF) of CRL4 required for centromeric loading and stabilization of CENP-A, giving it a defined enzymatic-complex role in centromere identity.

    Evidence Biochemical fractionation, quantitative imaging, RNAi of CUL4/DDB1/RBBP7 with CENP-A localization assays

    PMID:25795299

    Open questions at the time
    • Ubiquitination target relevant to CENP-A loading not identified
    • Structural basis of CENP-A recognition unresolved
  6. 2015 High

    Demonstrated that RBBP7-dependent histone deacetylation governs chromosomal passenger complex function and accurate chromosome segregation in oocytes, tying its chromatin role to meiotic fidelity.

    Evidence siRNA/morpholino knockdown in mouse oocytes with histone acetylation Western, CPC immunofluorescence, kinetochore-microtubule and SAC assays

    PMID:24317350

    Open questions at the time
    • Which HDAC complex mediates the relevant deacetylation not specified
    • Direct CPC-RBBP7 link not established
  7. 2015 Medium

    Extended RBBP7's chromatin-deacetylation role to reproductive tissue, showing it supports H4 acetylation-dependent cyclin D3 expression and decidualization under progesterone control.

    Evidence RNA knockdown in murine uterine stromal cells with H4 acetylation and cyclin D3 readouts and PR signaling demonstration

    PMID:26040671

    Open questions at the time
    • Direct chromatin targets at the cyclin D3 locus not mapped
    • Complex context for the acetylation effect unclear
  8. 2015 Medium

    Placed RBBP7 in an HDAC1/Sp1 promoter-bound repressor complex controlling RECK and metastasis, connecting it to Ras-driven invasion.

    Evidence ChIP, DAPA, RECK reporter, shRNA knockdown and xenograft lung metastasis model

    PMID:25885317

    Open questions at the time
    • Whether this complex is canonical NuRD/Sin3 not defined
    • Direct vs. indirect Ras regulation of RBBP7 unresolved
  9. 2016 High

    Showed the centromere-loading function of RBBP7 orthologs can be independent of histone acetylation and known chromatin complexes, revealing a chaperone activity distinct from its HDAC-complex roles.

    Evidence RNAi and epistasis with CENP-A(HCP-3) and KNL-2 in C. elegans holocentromeres

    PMID:26904949

    Open questions at the time
    • Molecular basis of acetylation-independent CENP-A handoff unknown
    • Direct mammalian confirmation of independence not shown
  10. 2017 Medium

    Identified AMPK as an upstream kinase that phosphorylates RBBP7 to enhance DNMT1 binding and inhibit DNA methylation, integrating metabolic signaling with chromatin/DNA-methylation control.

    Evidence Consensus-site identification, co-IP upon AMPK activation, pharmacological AMPK activation in HUVECs/mouse aorta and methylation assays

    PMID:28143904

    Open questions at the time
    • Phosphosite mutagenesis validation not detailed
    • Genome-wide methylation consequences not mapped
  11. 2017 Medium

    Provided structural insight into RBBP7 within NuRD by reconstituting and imaging the MTA2-RBBP7 sub-complex, defining its stoichiometry and architecture.

    Evidence Recombinant expression, affinity purification and negative stain EM 3D reconstruction

    PMID:28179136

    Open questions at the time
    • High-resolution structure not obtained
    • Functional consequence of the hinge motion untested
  12. 2018 Medium

    Defined a second CRL4-based role, with RBBP7 acting as the CRL4B DCAF that ubiquitinates HUWE1 and thereby controls MCL-1 and BRCA1 stability, expanding its E3-adaptor function to apoptosis and DNA-damage proteins.

    Evidence Co-IP, in vitro and cellular ubiquitination assays, gain/loss of function with HUWE1/MCL-1/BRCA1 Western blots

    PMID:29738775

    Open questions at the time
    • Structural basis of HUWE1 recognition unknown
    • Physiological contexts requiring this axis not delineated
  13. 2021 High

    Revealed paralog redundancy in development, with combined Rbbp4/Rbbp7 loss causing peri-implantation lethality and aberrant H3.3/H3K27ac, implicating RBBP7 in H3.3 deposition.

    Evidence siRNA double knockdown in mouse embryos with ChIP-seq, RNA-seq and lineage marker immunofluorescence

    PMID:34709113

    Open questions at the time
    • Direct biochemical role in H3.3 chaperoning not isolated from paralog
    • Which complex mediates the embryonic function unclear
  14. 2021 Medium

    Demonstrated a neuroprotective role through RBBP7-mediated reduction of p300 and tau acetylation/phosphorylation, extending its regulation of acetylation machinery to neurodegeneration.

    Evidence Viral overexpression in PS19 hippocampus and TauP301L cells with Western blot and survival/viability assays

    PMID:33978814

    Open questions at the time
    • Mechanism by which RBBP7 lowers p300 not defined
    • Direct vs. indirect effect on tau modification unresolved
  15. 2021 High

    Distinguished de novo from maintenance centromere assembly, showing RBBP7 ortholog and HAT-1 are needed for initial CENP-A deposition on artificial chromosomes versus a KNL-2-upstream maintenance pathway.

    Evidence RNAi, artificial chromosome microinjection and epistasis with CENP-A/KNL-2 in C. elegans

    PMID:33872374

    Open questions at the time
    • Mechanistic role of acetylation (HAT-1) in initial deposition not fully resolved
    • Mammalian de novo centromere relevance untested
  16. 2023 High

    Established the WD40-H4 binding function of RBBP7 as essential for human spermatogenesis through a causative truncating mutation and cross-species rescue, providing a direct disease link.

    Evidence Human variant identification, domain mapping, H4 co-IP, and Drosophila germline/cyst-cell RNAi with WT-vs-mutant human RBBP7 rescue

    PMID:37843278

    Open questions at the time
    • Downstream chromatin events in arresting spermatocytes not detailed
    • Penetrance and broader phenotype spectrum not established
  17. 2024 Medium

    Connected RBBP7 to oncogenic metabolism, showing SP1 drives RBBP7 expression to activate PI3K/AKT and the Warburg effect in hepatocellular carcinoma.

    Evidence Luciferase, ChIP, PI3K/AKT phosphorylation Western, proliferation and glycolysis assays

    PMID:38368381

    Open questions at the time
    • Mechanism linking RBBP7 to PI3K/AKT not defined
    • Direct vs. transcriptional effect unresolved
  18. 2025 Medium

    Uncovered new catalytic and adaptor activities: a covalently druggable Cys97 conferring E3-ligase-like degradation of diverse targets, a direct deacetylase role toward Acsl4-K401, and LSD1 recruitment to demethylate stemness gene promoters.

    Evidence Chemical proteomics with Cys97 mutagenesis (idx 22); acetyl-proteomics/IP-MS with Acsl4 mutant and activity assays (idx 23); co-IP and ChIP-qPCR with CRISPR and in vivo models (idx 24)

    PMID:40038738 PMID:40600340 PMID:41478474

    Open questions at the time
    • Whether endogenous RBBP7 acts as a bona fide E3 ligase outside engineered degraders unclear
    • Direct deacetylase catalytic mechanism for a WD40 protein not structurally resolved
    • Specificity determinants for LSD1 recruitment unmapped

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RBBP7's distinct activities — histone H4 chaperoning, HDAC-complex deacetylation, CRL4 substrate adaptation, AMPK-regulated DNMT1 binding, and the newly reported intrinsic deacetylase/E3 activities — are partitioned and regulated within a single WD40 protein remains unresolved.
  • No structural model reconciling chaperone vs. catalytic claims
  • Cell-type and complex context dictating which activity dominates not defined
  • Endogenous (non-engineered) catalytic activities require independent confirmation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 4 GO:0042393 histone binding 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0016874 ligase activity 2 GO:0060090 molecular adaptor activity 2
Localization
GO:0000228 nuclear chromosome 2 GO:0005634 nucleus 2
Pathway
R-HSA-4839726 Chromatin organization 4 R-HSA-1640170 Cell Cycle 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-392499 Metabolism of proteins 2
Partners
BRCA1CENP-ADNMT1HDAC1HISTONE H4HUWE1LSD1MTA2
Complex memberships
CRL4(RBBP7) / CRL4B E3 ubiquitin ligaseHDAC1/Sp1 promoter complexNuRD (MTA2-RBBP7)

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 RbAp46 (RBBP7) is a transcriptional target of Wilms' tumor suppressor WT1; WT1 upregulates RbAp46 ~15-fold, and RbAp46 overexpression inhibits cell growth and colony formation, establishing it as a growth inhibitor downstream of WT1. Suppression subtractive hybridization PCR to identify WT1 target genes; transfection of RbAp46 cDNA with colony formation and growth rate assays The Journal of biological chemistry Medium 9765217
2001 RbAp46 (RBBP7) interacts specifically with the BRCT domain of BRCA1 via its first two WD-repeats; this interaction represses BRCA1-mediated transactivation of the p21 promoter, and is disrupted by DNA-damaging agents. Yeast two-hybrid screening; co-immunoprecipitation; luciferase reporter assay for p21 promoter transactivation; domain mapping by deletion mutagenesis Biochemical and biophysical research communications Medium 11394910
2001 RbAp46 (RBBP7) overexpression in adenovirus-transformed HEK293 cells suppresses clonal growth in soft agar and tumor growth in nude mice, increases G2/M fraction, and augments apoptosis under serum starvation, indicating a role in cell cycle control and apoptosis. Stable transfection; soft agar colony formation; nude mouse xenograft; flow cytometry cell cycle analysis; serum starvation apoptosis assay International journal of cancer Medium 11433396
2003 Inducible RbAp46 expression activates the JNK (c-Jun NH2-terminal kinase) signaling pathway and triggers apoptosis; a dominant-negative JNK1 mutant blocks RbAp46-mediated apoptosis, placing RbAp46 upstream of JNK in this pathway. Tetracycline-inducible expression system; JNK activity assay; dominant-negative JNK1 epistasis; tumor xenograft in nude mice Anticancer research Medium 14981905
2003 Overexpression of RbAp46 in neoplastigenic MCF10AT3B breast epithelial cells constitutively activates the JNK pathway and GADD45, sensitizing cells to apoptosis induced by serum deprivation and hydrocortisone withdrawal. Stable cell line establishment; soft agar colony formation; nude mouse xenograft; Western blot for JNK and GADD45 activation International journal of cancer Medium 12767060
2004 Drosophila p55 (ortholog of RbAp46/RbAp48/RBBP7) is essential for repression of dE2F2-regulated target genes by the pRB-related proteins RBF1 and RBF2; its depletion deregulates E2F targets in a cell cycle-independent manner without affecting proliferation-coupled E2F targets, indicating mechanistically distinct repression modes. RNAi depletion of p55 in Drosophila cells; RT-PCR and reporter assays for E2F target gene expression; genetic epistasis with dE2F2/RBF complexes Molecular and cellular biology High 15456884
2006 RbAp46 expression in mammary epithelial cells upregulates GSK-3β expression, leading to increased phosphorylation and reduced steady-state levels of β-catenin and decreased β-catenin/TCF nuclear signaling. Stable cell line expressing RbAp46; Western blot for β-catenin phosphorylation and GSK-3β; TCF luciferase reporter assay; cell growth assay Anticancer research Low 17201172
2006 Constitutive RbAp46 expression in MCF10AT3B mammary epithelial cells induces epithelial-mesenchymal transition (EMT), characterized by downregulation of epithelial markers, upregulation of mesenchymal markers, and increased migration and invasion. Western blot and immunofluorescence for EMT markers; migration and invasion assays (Transwell) Anticancer research Low 17094482
2008 SUMO-1 physically interacts with RbAp46 and co-localizes in the nucleus; SUMO-1 overexpression increases RbAp46 protein stability (not at the transcriptional level and not via sumoylation of RbAp46), and this stabilization enhances RbAp46-mediated suppression of Ras-driven cell growth. Co-immunoprecipitation; co-localization by immunofluorescence; Western blot for protein levels with transcription and degradation controls; cell growth assay Anticancer research Medium 19189660
2015 RbAp46 (RBBP7) binds with HDAC1 and Sp1, and this complex binds to the RECK promoter at the Sp1 site, repressing RECK expression; Ras upregulates RbAp46 to suppress RECK and increase MMP-9 activity, promoting metastasis. Chromatin immunoprecipitation (ChIP); DNA affinity precipitation assay (DAPA); RECK reporter gene assay; lentiviral shRNA knockdown; xenograft lung metastasis model BMC cancer Medium 25885317
2015 RBBP7 is a substrate-specific adaptor (DCAF) for the CRL4 E3 ubiquitin ligase complex (CUL4/DDB1/ROC1); as CRL4(RBBP7), it is required for centromeric loading of newly synthesized CENP-A during G1 phase, in addition to binding and stabilizing soluble CENP-A. Biochemical fractionation; quantitative imaging; RNAi depletion of CUL4, DDB1, and RBBP7; CENP-A localization assays by fluorescence microscopy Journal of cell science Medium 25795299
2015 Rbbp7 knockdown in mouse oocytes prevents maturation-associated histone deacetylation, which in turn disrupts chromosomal passenger complex (CPC) localization and function, leading to chromosome misalignment, improper kinetochore-microtubule attachments, impaired spindle assembly checkpoint, cytokinesis defects, and aneuploidy at metaphase II. siRNA/morpholino knockdown; Western blot for histone acetylation; immunofluorescence for CPC components; kinetochore-microtubule attachment assays; spindle assembly checkpoint assay; aneuploidy scoring Cell cycle (Georgetown, Tex.) High 24317350
2015 Rbbp7 silencing in mouse uterine stromal cells attenuates histone H4 acetylation and reduces cyclin D3 expression, compromising stromal cell decidualization; Rbbp7 expression is induced by progesterone/nuclear receptor PR signaling. In situ hybridization; immunochemistry; stealth RNA knockdown; primary murine uterine stromal cell culture; in vitro decidualization model; Western blot Biology of reproduction Medium 26040671
2016 C. elegans RbAp46/48 (LIN-53, ortholog of RBBP7) is required for CENP-A(HCP-3) localization at holocentromeres; LIN-53 and CENP-A localizations are interdependent, and LIN-53 localization at the centromere during metaphase requires both CENP-A(HCP-3) and M18BP1(KNL-2). This function is independent of histone acetylation, H3K27 trimethylation, or known chromatin-modifying complexes. RNAi depletion; immunofluorescence for CENP-A and kinetochore components; epistasis analysis with KNL-2; anaphase bridge and chromosome missegregation scoring Cell reports High 26904949
2017 AMPK directly phosphorylates RBBP7 at an AMPK consensus sequence; this phosphorylation increases interaction between RBBP7 and DNMT1, contributing to inhibition of DNMT1-mediated DNA methylation and promoting nucleosome remodeling for mitochondrial gene expression. Identification of AMPK phosphorylation consensus in RBBP7; co-immunoprecipitation showing increased RBBP7-DNMT1 interaction upon AMPK activation; pharmacological AMPK activation in HUVECs and mouse aorta; methylation assays Science signaling Medium 28143904
2017 The human MTA2-RBBP7 sub-complex of the NuRD complex can be isolated as a stable complex in vitro; negative stain electron microscopy reveals an elongated architecture with hinge-like motion, consistent with the 2:4 stoichiometry (MTA:RBBP) seen in Drosophila NuRD. Recombinant expression in HEK293F cells; affinity purification; negative stain electron microscopy and 3D reconstruction Biochimica et biophysica acta. Proteins and proteomics Medium 28179136
2018 RBBP7 functions as the DCAF substrate receptor in the CRL4B (DDB1-CUL4B-ROC1) E3 ubiquitin ligase complex, bridging the complex to HUWE1 and mediating its polyubiquitination and proteasomal degradation; RBBP7 depletion stabilizes HUWE1 and accelerates degradation of MCL-1 and BRCA1 (HUWE1 substrates). Co-immunoprecipitation; ubiquitination assay in vitro and in cells; RBBP7 overexpression and depletion with Western blot for HUWE1, MCL-1, and BRCA1 levels Biochemical and biophysical research communications Medium 29738775
2021 Double knockdown of Rbbp4 and Rbbp7 (but not individual knockdowns) causes embryonic lethality at the morula-to-blastocyst transition in mice, with cell cycle block, disrupted lineage specification, and a dramatic increase in H3.3 and H3K27ac abundance; ChIP-seq reveals H3.3 enrichment at promoters of RBBP4/7 target genes, indicating compensatory roles in H3.3 deposition and chromatin regulation. siRNA double knockdown in preimplantation embryos; ChIP-seq for H3.3 and H3K27ac; RNA-seq for gene expression; immunofluorescence for lineage markers Epigenetics High 34709113
2021 Rbbp7 overexpression in hippocampal neurons reduces p300 levels, decreases tau acetylation at lysine 280, and reduces tau phosphorylation at AT8 and AT100 sites, thereby protecting against tau-induced neuronal death; in vitro Rbbp7 overexpression rescues TauP301L-induced cytotoxicity. Rbbp7 viral overexpression in PS19 mouse hippocampus; Western blot for p300, tau acetylation, and phosphorylation; neuronal survival quantification in CA1; cell viability assays in immortalized hippocampal cells and primary cortical neurons Acta neuropathologica Medium 33978814
2021 C. elegans RbAp46/48 (LIN-53) and HAT-1 are required for de novo CENP-A(HCP-3) and Mis18BP1(KNL-2) initial deposition at newly formed centromeres on artificial chromosomes; this requirement differs from centromere maintenance on endogenous chromosomes where KNL-2 acts upstream of LIN-53. RNAi depletion; microinjection of artificial chromosome constructs; immunofluorescence for CENP-A, KNL-2, and acetylation marks; epistasis analysis Nucleic acids research High 33872374
2023 A hemizygous truncating mutation in RBBP7 (affecting the sixth WD40 domain) abrogates interaction with histone H4 and causes maturation arrest in spermatogenesis; Drosophila Caf1-55 (RBBP7 ortholog) knockdown in germ cells eliminates germ cells, while knockdown in cyst cells causes hyperproliferative testicular cells, and male infertility is rescued by wild-type human RBBP7 but not mutant variants. Human genetic variant identification; domain mapping by mutation analysis; Co-IP for histone H4 interaction; γH2AX and BRCA1 Western blot; Drosophila germline-specific and cyst cell-specific RNAi; rescue with wild-type vs. mutant human RBBP7 The Journal of clinical investigation High 37843278
2024 SP1 transcription factor directly activates the RBBP7 promoter; RBBP7 in turn activates PI3K/AKT signaling to promote glycolysis (Warburg effect) and hepatocellular carcinoma cell proliferation. Dual-luciferase reporter assay for SP1-RBBP7 promoter; chromatin immunoprecipitation; Western blot for PI3K/AKT phosphorylation; CCK8 and colony formation; measurement of glycolytic enzymes and metabolites Journal of translational medicine Medium 38368381
2025 RBBP7 functions as a novel E3 ubiquitin ligase via covalent engagement at Cys97; ynamide-containing small-molecule degraders covalently engage Cys97 of RBBP7 to recruit it as the E3 ligase responsible for targeted degradation of multiple proteins including CDK4, PDE5, PI3K, AKT, BRD4, and mutant EGFR. Chemical proteomics (activity-based protein profiling); proteome profiling after degrader treatment; covalent labeling with ynamide electrophile; functional validation of target degradation by Western blot; site-specific mutagenesis (Cys97) Angewandte Chemie (International ed. in English) Medium 40600340
2025 RBBP7 directly deacetylates Acsl4 at lysine 401, reducing Acsl4 acetylation; deacetylation of Acsl4-K401 increases its enzyme activity by promoting ATP binding, thereby enhancing ferroptosis and promoting ovarian aging. Acetyl-proteomics; immunoprecipitation-mass spectrometry (IP-MS) identifying RBBP7 as direct Acsl4 deacetylation mediator; site-specific acetylation mutant analysis; Acsl4 enzyme activity assay; ferroptosis assays in vivo and in vitro International journal of biological macromolecules Medium 41478474
2025 RBBP7 interacts with LSD1 and recruits it to promoters of stemness genes (SOX9, SOX2, OCT4, CCND1) to erase repressive H3K9me3 marks, transcriptionally upregulating stemness gene expression and promoting breast cancer stem-like properties and metastasis. Co-immunoprecipitation for RBBP7-LSD1 interaction; ChIP-qPCR for H3K9me3 and LSD1 at stemness gene promoters; CRISPR/Cas9 deletion of RBBP7 super-enhancer; tumor sphere formation; tail vein metastasis model; PDO and PDX models Journal of translational medicine Medium 40038738

Source papers

Stage 0 corpus · 39 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1. Science signaling 205 28143904
2007 Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer. Molecular cancer research : MCR 66 17314274
2004 p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes. Molecular and cellular biology 56 15456884
1998 Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition. The Journal of biological chemistry 54 9765217
2001 Rb-associated protein 46 (RbAp46) suppresses the tumorigenicity of adenovirus-transformed human embryonic kidney 293 cells. International journal of cancer 35 11433396
2013 Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell cycle (Georgetown, Tex.) 33 24317350
2001 Rb-associated protein 46 (RbAp46) inhibits transcriptional transactivation mediated by BRCA1. Biochemical and biophysical research communications 30 11394910
2003 Overexpression of RbAp46 facilitates stress-induced apoptosis and suppresses tumorigenicity of neoplastigenic breast epithelial cells. International journal of cancer 27 12767060
2021 Overlapping functions of RBBP4 and RBBP7 in regulating cell proliferation and histone H3.3 deposition during mouse preimplantation development. Epigenetics 25 34709113
2015 CRL4(RBBP7) is required for efficient CENP-A deposition at centromeres. Journal of cell science 24 25795299
2015 Rbbp7 Is Required for Uterine Stromal Decidualization in Mice. Biology of reproduction 23 26040671
2020 Circular RNA hsa_circ_0006168 contributes to cell proliferation, migration and invasion in esophageal cancer by regulating miR-384/RBBP7 axis via activation of S6K/S6 pathway. European review for medical and pharmacological sciences 22 31957828
2016 RbAp46/48(LIN-53) Is Required for Holocentromere Assembly in Caenorhabditis elegans. Cell reports 22 26904949
2015 Ras induces experimental lung metastasis through up-regulation of RbAp46 to suppress RECK promoter activity. BMC cancer 22 25885317
2021 Identification of retinoblastoma binding protein 7 (Rbbp7) as a mediator against tau acetylation and subsequent neuronal loss in Alzheimer's disease and related tauopathies. Acta neuropathologica 20 33978814
2024 RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling. Journal of translational medicine 15 38368381
2022 Hypoxia-induced RBBP7 promotes esophagus cancer progression by inducing CDK4 expression. Acta biochimica et biophysica Sinica 14 35538026
2018 CRL4BRBBP7 targets HUWE1 for ubiquitination and proteasomal degradation. Biochemical and biophysical research communications 14 29738775
2006 Constitutive expression of RbAp46 induces epithelial-mesenchymal transition in mammary epithelial cells. Anticancer research 13 17094482
2003 Constitutive expression of Rb associated protein 46 (RbAp46) reverts transformed phenotypes of breast cancer cells. Anticancer research 13 14666671
2023 X-linked RBBP7 mutation causes maturation arrest and testicular tumors. The Journal of clinical investigation 10 37843278
2017 Expression, purification and characterization of the human MTA2-RBBP7 complex. Biochimica et biophysica acta. Proteins and proteomics 9 28179136
2003 Inducible expression of RbAp46 activates c-Jun NH2-terminal kinase-dependent apoptosis and suppresses progressive growth of tumor xenografts in nude mice. Anticancer research 9 14981905
2008 SUMO-1 overexpression increases RbAp46 protein stability and suppresses cell growth. Anticancer research 8 19189660
2023 FIT links c-Myc and P53 acetylation by recruiting RBBP7 during colorectal carcinogenesis. Cancer gene therapy 7 37225855
2021 RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos. Nucleic acids research 7 33872374
2007 RbAp46 inhibits estrogen-stimulated progression of neoplastigenic breast epithelial cells. Anticancer research 7 17970062
2025 Super-enhancer-hijacking RBBP7 potentiates metastasis and stemness of breast cancer via recruiting NuRD complex subunit LSD1. Journal of translational medicine 5 40038738
2025 Chemical Proteomics Identifies RBBP7 as a New E3 Ligase Supporting Targeted Protein Degradation. Angewandte Chemie (International ed. in English) 4 40600340
2020 The multifaceted histone chaperone RbAp46/48 in Plasmodium falciparum: structural insights, production, and characterization. Parasitology research 4 32363442
2022 Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy. Journal of immunology research 3 35958876
2006 Retinoblastoma suppressor associated protein 46 (RbAp46) attenuates the beta-catenin/TCF signaling through up-regulation of GSK-3beta expression. Anticancer research 3 17201172
2005 [Detection of RbAp46 expression in bone marrow cells of leukemia patients by real-time quantitative RT-PCR]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 2 16251025
2025 Identification of Aging-Related Hub Genes (ATP11B, RBBP7, DOCK10, and NUP160) as Potential Biomarkers and Therapeutic Targets in Sepsis. Human mutation 1 41394771
2006 [RbAp46 gene activates the expression of IGFBP-rP1 gene in K562 leukemic cells]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 1 16732965
2004 [Establishment and characterization of leukemic cell line U937 stably expressing exogenous RbAp46]. Zhongguo shi yan xue ye xue za zhi 1 15363122
2025 BAP1 complexes with YY1 and RBBP7 and its downstream targets in ccRCC cells. Open life sciences 0 40688406
2025 The Dual Role of RBBP7 in Esophageal Squamous Cell Carcinoma: Cell Context-Dependent Impacts on Proliferation and Radiosensitivity. OncoTargets and therapy 0 40934061
2025 Rbbp7-mediated deacetylation of Acsl4 promotes ovarian aging by enhancing ferroptosis. International journal of biological macromolecules 0 41478474

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