Affinage

RBBP7

Histone-binding protein RBBP7 · UniProt Q16576

Round 2 corrected
Length
425 aa
Mass
47.8 kDa
Annotated
2026-04-28
69 papers in source corpus 31 papers cited in narrative 27 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBBP7 (RbAp46) is a WD40-repeat histone-binding protein that serves as a shared scaffold subunit within multiple chromatin-regulatory complexes—including NuRD, Sin3-HDAC, PRC2, and CAF-1—where it bridges histone substrates (primarily H4) to enzymes that deacetylate, methylate, or remodel chromatin, thereby mediating transcriptional repression and epigenetic gene regulation (PMID:9150135, PMID:10444591, PMID:12435631). RBBP7 additionally functions as a DDB1-CUL4-associated factor (DCAF) substrate receptor in CRL4 E3 ubiquitin ligase complexes, directing ubiquitin-dependent CENP-A loading at centromeres and proteasomal degradation of targets such as HUWE1 (PMID:25795299, PMID:29738775). AMPK-mediated phosphorylation of RBBP7 enhances its inhibitory interaction with DNMT1, coupling metabolic sensing to DNA methylation and mitochondrial biogenesis gene expression (PMID:28143904). A hemizygous loss-of-function variant in X-linked RBBP7 that disrupts histone H4 binding causes maturation arrest azoospermia in humans, with cross-species rescue confirming a cell-autonomous requirement in germ cell survival (PMID:37843278).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1997 High

    The identification of RBBP7 as the histone-binding subunit that tethers the mSin3–HDAC1/2 co-repressor complex to chromatin established its foundational role as a histone chaperone bridging enzymes to their nucleosomal substrates.

    Evidence Biochemical purification and co-immunoprecipitation of the mSin3 complex from mammalian cells with functional transcription repression assays

    PMID:9150135

    Open questions at the time
    • Structural basis of the RBBP7–histone H4 interaction was not resolved
    • Whether RBBP7 contributes catalytic selectivity versus mere tethering was unclear
  2. 1998 High

    Demonstrating that RBBP7 is a core subunit shared between the NuRD and Sin3-HDAC complexes—and not exclusive to one—reframed it as a general-purpose HDAC-delivery module used by multiple remodeling machines.

    Evidence Biochemical purification and mass spectrometry of the NuRD complex from multiple labs, showing RBBP7/RBBP4 as shared HDAC core components

    PMID:10444591 PMID:9804427 PMID:9885572

    Open questions at the time
    • How specificity among NuRD versus Sin3 is achieved despite shared RBBP7 remained unknown
    • Stoichiometry within each complex was unresolved
  3. 2002 High

    The discovery that RBBP7 is an integral subunit of PRC2 with histone H3K27/K9 methyltransferase activity extended its functional repertoire beyond deacetylation to histone methylation-mediated Polycomb silencing.

    Evidence Biochemical reconstitution of PRC2 and in vitro HMT assay with SET-domain mutagenesis

    PMID:12435631

    Open questions at the time
    • Whether RBBP7 contributes allosterically to PRC2 catalysis or acts solely as a histone-presenting platform was not distinguished
  4. 2004 High

    RNAi depletion of the Drosophila ortholog p55 revealed that RBBP7 is selectively required for RBF/E2F2-mediated repression of differentiation genes but dispensable for E2F1-driven proliferation targets, establishing functional specificity in gene repression.

    Evidence RNAi knockdown in Drosophila cells with RT-PCR of specific E2F target gene classes

    PMID:15456884

    Open questions at the time
    • Mechanism distinguishing RBBP7-dependent from RBBP7-independent E2F repression was not identified
  5. 2013 High

    Linking RBBP7 to chromosome segregation fidelity, its depletion during oocyte maturation impaired histone deacetylation, chromosomal passenger complex localization, and spindle checkpoint function, causing aneuploidy.

    Evidence siRNA/morpholino knockdown in mouse oocytes with immunofluorescence of CPC components and chromosome segregation analysis

    PMID:24317350

    Open questions at the time
    • Which HDAC-containing complex mediates the maturation-specific deacetylation was not determined
    • Whether RBBP7 acts independently of RBBP4 in this context was unclear
  6. 2015 High

    RBBP7 was shown to function as a DCAF substrate receptor in CRL4 E3 ubiquitin ligase complexes required for ubiquitin-dependent CENP-A loading at centromeres during G1, revealing an unexpected non-chromatin-remodeling role in centromere maintenance.

    Evidence RNAi depletion with quantitative CENP-A imaging and biochemical co-immunoprecipitation of CRL4 components in human cells

    PMID:25795299

    Open questions at the time
    • The direct ubiquitination substrate at centromeres was not identified
    • Whether the CRL4-RBBP7 complex has substrates beyond CENP-A loading remained open
  7. 2016 High

    Cross-species conservation of RBBP7's centromere function was established when C. elegans LIN-53 depletion phenocopied CENP-A mislocalization and chromosome missegregation at holocentromeres, independent of known chromatin-modifying activities.

    Evidence RNAi in C. elegans with immunofluorescence and epistasis analysis with KNL-2 and CENP-A

    PMID:26904949

    Open questions at the time
    • The molecular mechanism by which RBBP7 promotes CENP-A deposition independently of its HDAC/PRC2 roles was not resolved
  8. 2017 High

    AMPK was identified as a direct kinase for RBBP7, and phosphorylation enhanced RBBP7's inhibitory interaction with DNMT1, establishing a signaling axis from metabolic sensing through epigenetic modification to mitochondrial gene expression.

    Evidence Biochemical phosphorylation assay, co-immunoprecipitation, pharmacological AMPK activation in HUVECs, and AMPK-knockout mouse aorta studies

    PMID:28143904

    Open questions at the time
    • The specific phosphorylation sites were not mapped at single-residue resolution
    • Whether AMPK-phosphorylated RBBP7 is selectively excluded from NuRD/PRC2 was not tested
  9. 2018 Medium

    RBBP7's DCAF function was extended to proteasomal degradation of HUWE1 via CRL4B, with downstream consequences for MCL-1 and BRCA1 stability, linking RBBP7 to apoptotic and DNA damage response regulation through ubiquitin-dependent proteolysis.

    Evidence Co-immunoprecipitation, ubiquitination assays, reciprocal overexpression/depletion of RBBP7 with monitoring of HUWE1, MCL-1, and BRCA1 protein levels

    PMID:29738775

    Open questions at the time
    • Single-lab finding not yet independently confirmed
    • Whether RBBP7's CRL4 adaptor role extends to additional substrates was not explored systematically
  10. 2021 High

    Redundancy between RBBP7 and RBBP4 was resolved by showing that double, but not single, knockdown causes embryonic lethality at the morula-to-blastocyst transition with aberrant H3.3 deposition and hyperacetylation, revealing compensatory roles in restricting variant histone incorporation during preimplantation development.

    Evidence siRNA knockdown in mouse embryos with RNA-seq, ChIP-seq for H3.3 and H3K27ac, and immunofluorescence

    PMID:34709113

    Open questions at the time
    • Whether H3.3 accumulation is a direct consequence of RBBP4/7 loss or secondary to HDAC/PRC2 dysfunction was not distinguished
    • Individual contributions of RBBP7 versus RBBP4 at specific loci remain unresolved
  11. 2023 High

    A hemizygous loss-of-function RBBP7 variant causing maturation arrest azoospermia in humans provided definitive genetic evidence that histone H4 binding by the sixth WD40 domain is essential for germ cell survival, confirmed by cross-species rescue in Drosophila.

    Evidence Human genetic variant identification, disrupted RBBP7–H4 co-immunoprecipitation, siRNA in spermatogonial cells, Drosophila germ-cell-specific knockdown with transgenic rescue by wild-type but not mutant human RBBP7

    PMID:37843278

    Open questions at the time
    • Which chromatin complex is critically disrupted in germ cells was not determined
    • Whether female fertility is similarly affected by RBBP7 hemizygosity is unknown
  12. 2025 Medium

    RBBP7 was found to recruit LSD1 to stemness gene promoters in breast cancer, erasing H3K9me3 to activate SOX2/SOX9/OCT4 transcription, and separately to mediate deacetylation of the non-histone substrate Acsl4, enhancing ferroptosis in ovarian aging—expanding its functional scope to demethylation recruitment and non-histone substrate regulation.

    Evidence ChIP-qPCR, CRISPR super-enhancer deletion, PDX/PDO models for stemness; IP-MS, acetyl-proteomics, and ferroptosis assays for Acsl4 deacetylation

    PMID:40038738 PMID:41478474

    Open questions at the time
    • Whether RBBP7 recruits LSD1 independently of NuRD is not resolved
    • The deacetylase that RBBP7 recruits to Acsl4 was not identified
    • Both findings from single labs await independent confirmation

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include which specific complex contexts mediate RBBP7's distinct functions (centromere assembly, germ cell survival, ferroptosis), how post-translational modifications partition RBBP7 among competing complexes, and whether a high-resolution structure of full-length RBBP7 within NuRD or CRL4 can explain its multivalent substrate recognition.
  • No high-resolution cryo-EM structure of RBBP7 within intact NuRD or CRL4 complex
  • Post-translational modification map (beyond AMPK phosphorylation) not systematically determined
  • Relative contribution of RBBP7 versus RBBP4 in individual complex contexts remains unclear

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0042393 histone binding 7 GO:0140110 transcription regulator activity 3 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 7 GO:0005694 chromosome 4
Pathway
R-HSA-4839726 Chromatin organization 7 R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-74160 Gene expression (Transcription) 3
Partners
CENP-ACUL4BDDB1DNMT1HDAC1HDAC2LSD1MTA2
Complex memberships
CRL4NuRDPRC2Sin3-HDAC

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 RbAp46 (RBBP7) and RbAp48 are components of the NuRD multisubunit complex, which contains both ATP-dependent nucleosome remodeling and histone deacetylase (HDAC1/2) activities; RbAp46/48 form a core histone deacetylase complex shared between NuRD and Sin3-HDAC complexes, and MTA2 modulates the enzymatic activity of this core complex. Biochemical purification, co-immunoprecipitation, mass spectrometry identification of complex subunits Genes & development High 10444591 9790534 9804427 9885572
1997 RbAp46/RbAp48 (RBBP7/RBBP4) are histone-binding proteins that tether the mSin3 co-repressor complex (containing HDAC1 and HDAC2) to core histone proteins, enabling transcriptional repression. Biochemical purification, co-immunoprecipitation, functional transcription repression assay Cell High 9150135
1999 RbAp46/48 are subunits of the Mi-2/NuRD complex in Xenopus and human cells, which couples DNA methylation to histone deacetylation and chromatin remodeling; MBD2 directs the complex to methylated DNA. Biochemical purification from Xenopus egg extracts and human cell lines, subunit identification by mass spectrometry and cloning Nature genetics High 10471499 10471500
1998 RbAp46 (RBBP7) is transcriptionally induced ~15-fold by the Wilms' tumor suppressor WT1, and its overexpression inhibits cell growth and colony formation, identifying it as a mediator of WT1-dependent growth inhibition. Suppression subtractive hybridization PCR to identify WT1 target genes; colony formation and growth rate assays in transfected cells The Journal of biological chemistry Medium 9765217
2001 RbAp46 (RBBP7) interacts specifically with the BRCT domain of BRCA1 via its first two WD-repeat domains, and represses BRCA1-mediated transactivation of the p21 promoter; this interaction is disrupted by DNA-damaging agents. Yeast two-hybrid screening, co-immunoprecipitation, luciferase transactivation assay, domain mapping Biochemical and biophysical research communications Medium 11394910
2001 RbAp46 (RBBP7) overexpression suppresses clonal growth of HEK293 cells in soft agar, inhibits tumor growth in nude mice, increases the G2/M cell fraction, and augments apoptosis in serum-starved cells. Soft agar colony formation, nude mouse xenograft, cell cycle analysis (flow cytometry), apoptosis assay International journal of cancer Medium 11433396
2002 RbAp46/RbAp48 (RBBP7/RBBP4) are components of the PRC2 (Polycomb repressive complex 2), which contains Enhancer of Zeste, Extra sex combs, and Su(z)12, and possesses histone methyltransferase activity specific for H3K9 and H3K27; the HMT activity requires an intact SET domain in E(z). Biochemical purification, co-immunoprecipitation, histone methyltransferase assay, domain mutagenesis Genes & development High 12435631
2003 RbAp46 (RBBP7) inducible expression activates the c-Jun NH2-terminal kinase (JNK) pathway and triggers apoptosis; a dominant-negative JNK1 mutant blocks RbAp46-mediated apoptosis, placing RbAp46 upstream of JNK in a pro-apoptotic pathway. Tetracycline-inducible expression system, JNK kinase assay, dominant-negative JNK1 epistasis, xenograft tumor model Anticancer research Medium 14981905
2004 The Drosophila ortholog of RbAp46/48, p55/dCAF-1, is essential for repression of dE2F2-regulated target genes by RBF1 and RBF2 complexes; RNAi depletion of p55 specifically deregulates cell cycle-independent E2F2 targets without affecting proliferation-coupled E2F1 targets, revealing mechanistically distinct repression pathways. RNAi depletion in Drosophila cells, RT-PCR of E2F target genes, genetic epistasis analysis Molecular and cellular biology High 15456884
2006 RbAp46 (RBBP7) expression in mammary epithelial cells increases GSK-3β expression, leading to hyperphosphorylation and reduced steady-state levels of β-catenin, thereby suppressing β-catenin/TCF nuclear signaling and cell growth. Stable transfection, Western blot, luciferase reporter assay for β-catenin/TCF signaling Anticancer research Low 17201172
2008 SUMO-1 forms a protein complex with RbAp46 (RBBP7) in the nucleus (co-localization and co-immunoprecipitation), stabilizing RbAp46 protein levels post-translationally without sumoylating RbAp46 directly; SUMO-1-mediated stabilization of RbAp46 enhances suppression of Ras-induced cell proliferation. Co-immunoprecipitation, co-localization imaging, Western blot, cell growth assays Anticancer research Low 19189660
2013 RBBP7 is a dormant maternal mRNA recruited for translation during mouse oocyte maturation to regulate histone deacetylation; RBBP7 depletion by siRNA/morpholino impairs maturation-associated histone deacetylation, which in turn is required for chromosomal passenger complex (CPC) localization and function, causing chromosome misalignment, improper kinetochore-microtubule attachments, impaired spindle assembly checkpoint, cytokinesis defects, and increased aneuploidy at metaphase II. siRNA/morpholino knockdown, immunofluorescence of CPC components, histone acetylation assays, chromosome segregation analysis Cell cycle (Georgetown, Tex.) High 24317350
2015 RBBP7 acts as a substrate-specific adaptor (DCAF) in the CRL4 (CUL4-DDB1-ROC1) E3 ubiquitin ligase complex; CRL4(RBBP7) is required for ubiquitin-dependent loading of newly synthesized CENP-A at centromeres during G1, and RBBP7 also stabilizes soluble pre-nucleosomal CENP-A. RNAi depletion, quantitative imaging of CENP-A dynamics, biochemical co-immunoprecipitation, cell cycle staging Journal of cell science High 25795299
2015 Rbbp7 expression in mouse uterine stromal cells is induced by progesterone-nuclear receptor PR signaling; siRNA knockdown of Rbbp7 in primary murine stromal cells compromises decidualization by attenuating histone H4 acetylation and cyclin D3 expression. In situ hybridization, immunochemistry, siRNA knockdown, in vitro decidualization model, histone acetylation assay, Western blot Biology of reproduction Medium 26040671
2015 RbAp46 (RBBP7) is upregulated by oncogenic Ha-ras and forms a complex with HDAC1 and Sp1 that binds the RECK promoter at Sp1 sites, repressing RECK expression and thereby increasing MMP-9 activity and promoting lung metastasis in vivo. Suppression subtractive hybridization PCR, ChIP assay, DNA affinity precipitation, RECK reporter gene assay, shRNA knockdown, xenograft nude mouse model BMC cancer Medium 25885317
2016 RbAp46/48 (LIN-53), the C. elegans ortholog of RBBP7, is required for CENP-A(HCP-3) localization at holocentromeres; its centromeric localization during metaphase is interdependent with CENP-A and depends on M18BP1(KNL-2); depletion causes anaphase bridges and chromosome missegregation, and this function is independent of histone acetylation, H3K27 trimethylation, or known chromatin-modifying complexes. RNAi depletion in C. elegans, immunofluorescence of centromere/kinetochore proteins, epistasis analysis with KNL-2 and CENP-A Cell reports High 26904949
2017 AMPK directly phosphorylates RBBP7 at consensus phosphorylation sequences; AMPK-mediated phosphorylation of RBBP7 increases its interaction with and inhibition of DNMT1, contributing to reduced DNA methylation and increased expression of mitochondrial biogenesis genes (PGC-1α, Tfam, UCP2, UCP3). Consensus sequence analysis, biochemical phosphorylation assays, co-immunoprecipitation, pharmacological AMPK activation, pulsatile shear stress in HUVECs, mouse aorta studies with AMPKα2 knockout Science signaling High 28143904
2017 The human MTA2-RBBP7 sub-complex of NuRD can be isolated as a stable entity; negative stain electron microscopy reveals an elongated architecture capable of hinge-like motion, with stoichiometry analogous to the Drosophila NuRD MTA-RBBP complex (2:4 MTA:RBBP), suggesting MTA-RBBP is a stable core module for NuRD assembly. HEK293F cell expression, biochemical purification, negative stain electron microscopy, 3D reconstruction Biochimica et biophysica acta. Proteins and proteomics Medium 28179136
2018 RBBP7 is the substrate-receptor (DCAF) in the CRL4B E3 ubiquitin ligase complex (CRL4B-RBBP7) that bridges DDB1-CUL4B-ROC1 to HUWE1, mediating HUWE1 polyubiquitination and proteasomal degradation; RBBP7 overexpression promotes HUWE1 degradation, while RBBP7 depletion stabilizes HUWE1 and accelerates degradation of MCL-1 and BRCA1. Co-immunoprecipitation, overexpression/depletion experiments, ubiquitination assay, Western blot for substrate levels Biochemical and biophysical research communications Medium 29738775
2021 RBBP7 (as a NuRD complex subunit) physically interacts with p300; overexpression of Rbbp7 reduces p300 protein levels, decreases tau acetylation at K280, and reduces tau phosphorylation at AT8 and AT100 epitopes; hippocampal Rbbp7 overexpression rescues neuronal death in the CA1 of PS19 tauopathy mice. In vitro overexpression in immortalized hippocampal cells and primary cortical neurons, in vivo hippocampal viral overexpression, Western blot for tau acetylation/phosphorylation, co-immunoprecipitation (Rbbp7-p300 interaction) Acta neuropathologica Medium 33978814
2021 Double knockdown of Rbbp4 and Rbbp7 (but not individually) causes embryonic lethality during the morula-to-blastocyst transition in mice; RBBP4/7 depletion leads to cell cycle block, disrupted lineage specification, and a dramatic increase in H3.3 and H3K27ac abundance with H3.3 enrichment at promoters of RBBP4/7 target genes, revealing a compensatory role for RBBP4/7 in regulating histone H3.3 deposition during preimplantation development. siRNA knockdown in mouse embryos, RNA-seq, ChIP-seq, Western blot, immunofluorescence Epigenetics High 34709113
2021 In C. elegans, RbAp46/48 (LIN-53) and HAT-1 are required for histone H3 and H4 acetylation (H3K9ac, H4K5ac, H4K12ac), chromatinization of artificial chromosomes, and de novo CENP-A(HCP-3) and Mis18BP1(KNL-2) deposition at nascent centromeres; this requirement is distinct from centromere maintenance on endogenous chromosomes where Mis18BP1(KNL-2) acts upstream of RbAp46/48. RNAi depletion, immunofluorescence of centromeric and histone marks, artificial chromosome microinjection assay Nucleic acids research High 33872374
2023 A hemizygous loss-of-function variant in X-linked RBBP7 causes maturation arrest azoospermia; the mutation disrupts the sixth WD40 domain, abrogating interaction of RBBP7 with histone H4; in mouse spermatogonial and pachytene spermatocyte cells, Rbbp7 depletion causes cell cycle arrest and apoptosis with decreased BRCA1 and increased γH2AX; in Drosophila, germ-cell-specific knockdown of Caf1-55 eliminates germ cells (male infertility rescued by wild-type human RBBP7 but not mutant), while cyst-cell knockdown causes testicular hyperproliferation. Human genetic variant identification, co-immunoprecipitation (RBBP7-H4 interaction), siRNA knockdown in cell lines, flow cytometry, Western blot, Drosophila genetics with transgenic rescue The Journal of clinical investigation High 37843278
2025 RBBP7 promotes breast cancer stemness and metastasis by recruiting the NuRD complex subunit LSD1; RBBP7 interacts with LSD1 and relies on LSD1 to erase H3K9me3 repressive marks at promoters of stemness genes (SOX9, SOX2, OCT4, CCND1), thereby transcriptionally upregulating them; CRISPR/Cas9 deletion of the RBBP7 super-enhancer decreases RBBP7 expression and suppresses malignant features. ChIP-qPCR, dual-luciferase reporter assay, Co-IP (RBBP7-LSD1 interaction), CRISPR/Cas9 SE deletion, tail vein injection metastasis model, PDO/PDX models Journal of translational medicine Medium 40038738
2025 RBBP7 functions as an E3 ubiquitin ligase through covalent engagement of its Cys97 residue by ynamide electrophile-containing degrader compounds, enabling targeted protein degradation; chemical proteomics identified RBBP7 Cys97 as the covalent engagement site responsible for the degradation mechanism. Chemical proteomics (proteome profiling after ynamide compound treatment), functional validation of degradation, site-specific mutagenesis (Cys97) Angewandte Chemie (International ed. in English) Medium 40600340
2025 Rbbp7 mediates deacetylation of Acsl4 at lysine 401 (Acsl4-K401); this deacetylation increases Acsl4 enzyme activity by promoting ATP binding, thereby enhancing ferroptosis and promoting ovarian aging; Rbbp7 was identified as a direct mediator of Acsl4 deacetylation by immunoprecipitation-mass spectrometry. Acetyl-proteomic analysis, IP-MS identification of Rbbp7 as Acsl4 deacetylase, Western blot, in vivo/in vitro ferroptosis assays, pharmacological inhibition International journal of biological macromolecules Medium 41478474
2025 BAP1 forms a complex with YY1 and RBBP7 in clear cell renal cell carcinoma cells, and BAP1 knockdown alters gene expression profiles in ccRCC cells. Co-immunoprecipitation, gene expression profiling after BAP1 knockdown Open life sciences Low 40688406

Source papers

Stage 0 corpus · 69 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2002 Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein. Genes & development 1332 12435631
2010 Network organization of the human autophagy system. Nature 1286 20562859
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2006 Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. Molecular cell 1260 16916647
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2005 Nucleolar proteome dynamics. Nature 934 15635413
1999 Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes & development 914 10444591
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
1998 NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities. Molecular cell 825 9885572
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
1999 MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nature genetics 720 10471499
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
1998 The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities. Cell 701 9790534
2012 A census of human soluble protein complexes. Cell 689 22939629
1999 Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation. Nature genetics 679 10471500
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2010 Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. Cell 639 20850016
2011 Global landscape of HIV-human protein complexes. Nature 593 22190034
2006 The human CENP-A centromeric nucleosome-associated complex. Nature cell biology 593 16622419
1998 Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex. Nature 585 9804427
2002 ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Molecular cell 576 12453419
2009 LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer. Cell 564 19703393
2009 Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. Cell 555 19410544
2006 ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. Molecular cell 550 16387653
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2011 Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nature biotechnology 531 21258344
1997 Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex. Cell 506 9150135
2017 AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1. Science signaling 203 28143904
2007 Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer. Molecular cancer research : MCR 66 17314274
2004 p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes. Molecular and cellular biology 56 15456884
1998 Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition. The Journal of biological chemistry 54 9765217
2001 Rb-associated protein 46 (RbAp46) suppresses the tumorigenicity of adenovirus-transformed human embryonic kidney 293 cells. International journal of cancer 35 11433396
2013 Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell cycle (Georgetown, Tex.) 32 24317350
2001 Rb-associated protein 46 (RbAp46) inhibits transcriptional transactivation mediated by BRCA1. Biochemical and biophysical research communications 29 11394910
2003 Overexpression of RbAp46 facilitates stress-induced apoptosis and suppresses tumorigenicity of neoplastigenic breast epithelial cells. International journal of cancer 27 12767060
2021 Overlapping functions of RBBP4 and RBBP7 in regulating cell proliferation and histone H3.3 deposition during mouse preimplantation development. Epigenetics 25 34709113
2015 CRL4(RBBP7) is required for efficient CENP-A deposition at centromeres. Journal of cell science 24 25795299
2015 Rbbp7 Is Required for Uterine Stromal Decidualization in Mice. Biology of reproduction 23 26040671
2020 Circular RNA hsa_circ_0006168 contributes to cell proliferation, migration and invasion in esophageal cancer by regulating miR-384/RBBP7 axis via activation of S6K/S6 pathway. European review for medical and pharmacological sciences 22 31957828
2015 Ras induces experimental lung metastasis through up-regulation of RbAp46 to suppress RECK promoter activity. BMC cancer 22 25885317
2016 RbAp46/48(LIN-53) Is Required for Holocentromere Assembly in Caenorhabditis elegans. Cell reports 21 26904949
2021 Identification of retinoblastoma binding protein 7 (Rbbp7) as a mediator against tau acetylation and subsequent neuronal loss in Alzheimer's disease and related tauopathies. Acta neuropathologica 20 33978814
2022 Hypoxia-induced RBBP7 promotes esophagus cancer progression by inducing CDK4 expression. Acta biochimica et biophysica Sinica 14 35538026
2024 RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling. Journal of translational medicine 13 38368381
2018 CRL4BRBBP7 targets HUWE1 for ubiquitination and proteasomal degradation. Biochemical and biophysical research communications 13 29738775
2006 Constitutive expression of RbAp46 induces epithelial-mesenchymal transition in mammary epithelial cells. Anticancer research 13 17094482
2003 Constitutive expression of Rb associated protein 46 (RbAp46) reverts transformed phenotypes of breast cancer cells. Anticancer research 13 14666671
2023 X-linked RBBP7 mutation causes maturation arrest and testicular tumors. The Journal of clinical investigation 10 37843278
2017 Expression, purification and characterization of the human MTA2-RBBP7 complex. Biochimica et biophysica acta. Proteins and proteomics 9 28179136
2003 Inducible expression of RbAp46 activates c-Jun NH2-terminal kinase-dependent apoptosis and suppresses progressive growth of tumor xenografts in nude mice. Anticancer research 9 14981905
2008 SUMO-1 overexpression increases RbAp46 protein stability and suppresses cell growth. Anticancer research 8 19189660
2023 FIT links c-Myc and P53 acetylation by recruiting RBBP7 during colorectal carcinogenesis. Cancer gene therapy 7 37225855
2007 RbAp46 inhibits estrogen-stimulated progression of neoplastigenic breast epithelial cells. Anticancer research 7 17970062
2021 RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos. Nucleic acids research 6 33872374
2025 Super-enhancer-hijacking RBBP7 potentiates metastasis and stemness of breast cancer via recruiting NuRD complex subunit LSD1. Journal of translational medicine 4 40038738
2020 The multifaceted histone chaperone RbAp46/48 in Plasmodium falciparum: structural insights, production, and characterization. Parasitology research 4 32363442
2025 Chemical Proteomics Identifies RBBP7 as a New E3 Ligase Supporting Targeted Protein Degradation. Angewandte Chemie (International ed. in English) 3 40600340
2022 Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy. Journal of immunology research 3 35958876
2006 Retinoblastoma suppressor associated protein 46 (RbAp46) attenuates the beta-catenin/TCF signaling through up-regulation of GSK-3beta expression. Anticancer research 3 17201172
2005 [Detection of RbAp46 expression in bone marrow cells of leukemia patients by real-time quantitative RT-PCR]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 2 16251025
2025 Identification of Aging-Related Hub Genes (ATP11B, RBBP7, DOCK10, and NUP160) as Potential Biomarkers and Therapeutic Targets in Sepsis. Human mutation 1 41394771
2006 [RbAp46 gene activates the expression of IGFBP-rP1 gene in K562 leukemic cells]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 1 16732965
2004 [Establishment and characterization of leukemic cell line U937 stably expressing exogenous RbAp46]. Zhongguo shi yan xue ye xue za zhi 1 15363122
2025 BAP1 complexes with YY1 and RBBP7 and its downstream targets in ccRCC cells. Open life sciences 0 40688406
2025 The Dual Role of RBBP7 in Esophageal Squamous Cell Carcinoma: Cell Context-Dependent Impacts on Proliferation and Radiosensitivity. OncoTargets and therapy 0 40934061
2025 Rbbp7-mediated deacetylation of Acsl4 promotes ovarian aging by enhancing ferroptosis. International journal of biological macromolecules 0 41478474