Affinage

CUL4B

Cullin-4B · UniProt Q13620

Length
913 aa
Mass
104.0 kDa
Annotated
2026-06-09
100 papers in source corpus 41 papers cited in narrative 40 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CUL4B is the scaffold subunit of a cullin-RING E3 ubiquitin ligase (CRL4B) that assembles with DDB1, RBX1/ROC1, and interchangeable DCAF substrate receptors to drive both proteasomal degradation of specific substrates and epigenetic gene silencing through chromatin modification (PMID:17273978, PMID:37679762). A nonsense mutation truncating its C-terminal catalytic domain abolishes ligase function and causes X-linked intellectual disability, establishing the C-terminus as essential for catalysis (PMID:17273978). As a degradative ligase, CRL4B targets a broad substrate set in a NEDD8-dependent manner — cyclin E and TSC2 (the latter activating mTOR signaling) (PMID:23348097), p53 (PMID:25464270, PMID:39695153), the COP9 signalosome component Jab1/CSN5 (PMID:23357576), HUWE1 (PMID:25883150), PPARγ (PMID:27899484), INSL6 (PMID:26846852), MEN1 via DCAF7 (PMID:36939378), the histone demethylase UTX via a DDB1-COP1 module (PMID:37679762), and ARID1A (PMID:40203790) — coupling it to cell-cycle progression, metabolism, stress responses, and tumor differentiation. In parallel, CRL4B acts as a chromatin-modifying repressor: it catalyzes monoubiquitination of H2AK119 and coordinates with PRC2 (H3K27me3) and HDAC complexes to silence tumor-suppressor microRNAs and target genes, including miR-194 (a reciprocal feedback partner) (PMID:28164432), miR-372/373 (PMID:32127645), miR-34a (PMID:32054830), and the CDKN1A/p21 promoter (PMID:33869025), thereby activating oncogenic Wnt/β-catenin, PI3K/AKT, and Notch pathways (PMID:25430888, PMID:32127645). CUL4B carries a unique N-terminal NLS (KKRK, aa 37-40) that binds importin α isoforms and is required for nuclear localization and proliferation (PMID:19801544), and its N-terminal extension is heavily phosphorylated during mitosis to recruit the noncanonical DCAFs LIS1 and WDR1, controlling spindle positioning and forebrain development (PMID:37365982). CUL4B is essential for mouse embryonic development through a requirement in extra-embryonic tissues (PMID:22453236, PMID:22606329), and in neural progenitors its loss derepresses PP2A regulatory subunits PPP2R2B/PPP2R2C, inhibiting AKT/ERK to cause premature cell-cycle exit and precocious neuronal differentiation underlying X-linked intellectual disability (PMID:38331954).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2007 Medium

    Established CUL4B as a cullin-RING E3 ligase scaffold whose C-terminal catalytic domain is essential, linking its loss of function to human disease.

    Evidence Mutation analysis of an XLID nonsense allele (p.R388X) with NMD and X-inactivation analysis in patient cells

    PMID:17273978

    Open questions at the time
    • Did not define substrate receptors or specific substrates
    • Mechanism connecting ligase loss to intellectual disability not addressed
  2. 2009 High

    Resolved why CUL4B differs from other cullins by mapping a unique N-terminal NLS required for nuclear localization and proliferation, and identified cyclin E as a degradation substrate.

    Evidence NLS deletion mutants, importin α1/α3/α5 binding assays, subcellular fractionation, RNAi and cell-cycle analysis

    PMID:19801544

    Open questions at the time
    • DCAF receptor mediating cyclin E recognition not identified
    • Did not address how nuclear/cytoplasmic partitioning is regulated
  3. 2012 High

    Demonstrated that CUL4B is required for embryonic viability and localized its essential function to extra-embryonic tissues, distinguishing it from the embryo-intrinsic role.

    Evidence Conventional and epiblast-specific (Sox2-Cre) Cul4b knockout mice with cyclin E immunoblotting

    PMID:22453236 PMID:22606329

    Open questions at the time
    • Extra-embryonic substrate(s) driving lethality not defined
    • Mechanistic basis of tissue specificity unresolved
  4. 2013 High

    Expanded the degradative substrate repertoire (Jab1/CSN5, TSC2, cyclin E) and connected CRL4B to mTOR signaling and DNA replication licensing through CDC6/CDK2.

    Evidence In vitro/in vivo ubiquitination assays, Co-IP, Cul4b-deficient MEFs, neocortical neuron expression, chromatin fractionation, miRNA manipulation

    PMID:23348097 PMID:23357576 PMID:23479742

    Open questions at the time
    • DCAF receptors for these substrates not specified
    • Whether degradative and replication-licensing functions are mechanistically separable unknown
  5. 2014 High

    Identified p53 as a CRL4B substrate, defining a role in suppressing stress-induced senescence via a p53-ROS feedback loop.

    Evidence Gain/loss-of-function with p53 ubiquitination assay, H2O2 treatment, ROS and senescence β-gal readouts in fibroblasts

    PMID:25464270

    Open questions at the time
    • Distinction between stress-induced and replicative senescence regulation not fully mechanistic
    • DCAF receptor for p53 not identified
  6. 2015 High

    Established CRL4B as an epigenetic repressor that recruits PRC2 to silence pathway antagonists, and extended its developmental roles to neural progenitor astrocytogenesis and HUWE1-controlled apoptosis.

    Evidence ChIP for PRC2/H3K27me3, rescue/epistasis experiments, nervous-system-specific KO mice, pharmacological PTGDS inhibition, in vitro ubiquitination

    PMID:25430888 PMID:25883150 PMID:26025376

    Open questions at the time
    • Mechanism of PRC2 recruitment to specific loci unresolved
    • Relationship between degradative and chromatin-silencing activities at the complex level unclear
  7. 2016 High

    Defined tissue-specific physiological roles via conditional knockouts (adipocyte PPARγ regulation; germ cell INSL6 degradation in spermatogenesis), with mass-spectrometry substrate identification.

    Evidence Adipocyte- and germ-cell-specific conditional KO mice, ubiquitination assays, mass spectrometry, metabolic and sperm phenotyping, EM of flagella

    PMID:26846852 PMID:27899484

    Open questions at the time
    • DCAF receptors for PPARγ and INSL6 not defined
    • Non-cell-autonomous niche mechanism in spermatogenesis incompletely resolved
  8. 2017 High

    Demonstrated that CRL4B directly catalyzes H2AK119 monoubiquitination at microRNA loci, establishing a catalytic chromatin mechanism and a miR-194 double-negative feedback loop downstream of p53.

    Evidence ChIP for H2AK119ub/H3K27me3 at miR-194 cluster, 3'-UTR luciferase reporter, RNAi, xenografts

    PMID:28164432

    Open questions at the time
    • How H2AK119ub coordinates with PRC2 H3K27me3 mechanistically unresolved
    • Locus-specificity determinants of CRL4B chromatin targeting unknown
  9. 2019 High

    Revealed immune-regulatory roles in which CRL4B epigenetically tunes myeloid and T-cell programs (PTEN, IL-6, miR-372/373, miR-194-5p) and defined DCAF1 as the T-cell substrate receptor linked to DNA-damage response.

    Evidence Myeloid- and T-cell-specific conditional KOs, ChIP at target promoters, pharmacological/blocking rescues, Co-IP and mass spectrometry, DNA damage and SMC1A phosphorylation assays

    PMID:31235785 PMID:31729464 PMID:32127645 PMID:33524014 PMID:37224018

    Open questions at the time
    • How CRL4B switches between degradative DCAF1-dependent and chromatin-repressive modes unclear
    • Direct chromatin substrates versus indirect effects not always distinguished
  10. 2021 High

    Broadened CRL4B oncogenic and viral roles (tamoxifen resistance via miR-32-5p/ER-α36, HBx stabilization to promote HBV, TMZ resistance via CDKN1A silencing) and refined nuclear import via THAP7-AS1 lncRNA enhancing NLS-importin α1 binding.

    Evidence ChIP at miR/promoter loci, dominant-negative and KD/OE mutants, Co-IP/RIP/IF, cycloheximide chase, in vivo HBV and tumor models

    PMID:33638154 PMID:33869025 PMID:33969670 PMID:34146543 PMID:34608273

    Open questions at the time
    • Generality of lncRNA-assisted nuclear import beyond tested contexts unknown
    • Whether HBx stabilization reflects substrate competition or altered complex composition unresolved
  11. 2023 High

    Connected mitosis-specific N-terminal phosphorylation to recruitment of noncanonical DCAFs (LIS1, WDR1) controlling spindle positioning, and defined additional DCAF-based substrate complexes (DCAF7-MEN1, DDB1-COP1-UTX) and tumor-microenvironment chromatin targets (Cxcl2, Csf3).

    Evidence Phosphoproteomics, Co-IP of LIS1/WDR1/DCAF7/COP1 with DDB1, mutagenesis, live imaging, forebrain organoids, ChIP, conditional KO tumor models

    PMID:36939378 PMID:37365982 PMID:37653114 PMID:37679762 PMID:37925424 PMID:38761506

    Open questions at the time
    • Kinase(s) phosphorylating the N-terminal extension not identified
    • Structural basis of phosphorylation-dependent DCAF selection unresolved
  12. 2024 High

    Provided a patient-iPSC mechanistic model of XLID showing that CRL4B represses PP2A regulatory subunits (PPP2R2B/C) via H2AK119ub, sustaining AKT/ERK to prevent premature neuronal differentiation, and added p53/PSME3-dependent radiation and kidney-injury roles.

    Evidence Patient iPSC-derived neurons/organoids, ChIP for H2AK119ub, PP2A activity assay, AKT/ERK and PP2A pharmacological rescue, kidney conditional KO injury models, Co-IP for CUL4B-PSME3

    PMID:38331954 PMID:38689033 PMID:39695153

    Open questions at the time
    • How a single XLID lesion produces both degradative and epigenetic deficits not integrated
    • PSME3 relocalization to γH2AX foci mechanism incompletely defined (Medium-confidence)
  13. 2025 High

    Extended CRL4B substrate degradation to chromatin-remodeling factor ARID1A, linking it to thyroid cancer dedifferentiation via PAX8 downregulation.

    Evidence Co-IP and ubiquitination assay for CUL4B-ARID1A, in vivo thyroid cancer models, RNA-seq, IHC

    PMID:40203790

    Open questions at the time
    • DCAF receptor recognizing ARID1A not identified
    • Whether ARID1A degradation and chromatin repression act on overlapping gene programs unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how the CRL4B complex partitions between its degradative substrate-receptor repertoire and its catalytic chromatin-silencing activity, and what determines locus- and substrate-specific targeting in different tissues.
  • No structural model integrating DCAF substrate selection with H2AK119ub chromatin function
  • DCAF receptors for most degradative substrates remain unidentified
  • Rules governing context-specific microRNA/gene-locus targeting unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 8 GO:0140110 transcription regulator activity 5 GO:0016874 ligase activity 4 GO:0042393 histone binding 3 GO:0060090 molecular adaptor activity 3
Localization
GO:0000228 nuclear chromosome 4 GO:0005634 nucleus 2 GO:0005829 cytosol 1
Pathway
R-HSA-392499 Metabolism of proteins 8 R-HSA-1266738 Developmental Biology 5 R-HSA-162582 Signal Transduction 5 R-HSA-168256 Immune System 5 R-HSA-4839726 Chromatin organization 5 R-HSA-1640170 Cell Cycle 4 R-HSA-1643685 Disease 4
Complex memberships
CRL4B (CUL4B-DDB1-RBX1/ROC1)CUL4B-DCAF1CUL4B-DDB1-COP1CUL4B-PRC2

Evidence

Reading pass · 40 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2009 CUL4B contains a nuclear localization signal (NLS) in its N-terminus (amino acids 37-40, KKRK) that binds importin α1, α3, and α5. Unlike CUL4A and other cullins which carry NLS in their C-termini, this N-terminal NLS is unique to CUL4B. NLS-deleted CUL4B localizes to the cytoplasm and fails to promote cell proliferation. CUL4B promotes ubiquitination and degradation of cyclin E, and RNAi silencing leads to overaccumulation of cyclin E, prolonged S phase, and inhibited cell proliferation. RNAi silencing, NLS deletion mutants, importin binding assays, subcellular fractionation, cell cycle analysis The Journal of biological chemistry High 19801544
2007 CUL4B is a scaffold protein that organizes a cullin-RING (E3) ubiquitin ligase complex. A nonsense mutation (p.R388X) truncates the C-terminal catalytic domain and causes nonsense-mediated mRNA decay, demonstrating that the C-terminal domain is essential for ligase function. Mutation analysis, mRNA expression in patient leukocytes, X-chromosome inactivation analysis American journal of human genetics Medium 17273978
2013 CUL4B promotes DNA replication licensing by positively regulating CDC6 (a replication licensing factor) through a CDK2-dependent mechanism: CUL4B represses miR-372 and miR-373, which target CDK2, thereby elevating CDK2 levels; CDK2 then phosphorylates CDC6, protecting it from APC(CDH1)-mediated degradation, which in turn promotes loading of MCM2 onto chromatin. RNAi knockdown, chromatin fractionation for MCM2 loading, miRNA overexpression/inhibition, immunoblotting The Journal of cell biology High 23479742
2013 CUL4B ubiquitin ligase targets Jab1/CSN5 (a component of the COP9 signalosome) for polyubiquitination and proteasomal degradation. The DDB1-CUL4B-ROC1 complex is required for this degradation, which is independent of CUL4A. Loss of CUL4B leads to Jab1 accumulation and abnormal upregulation of BMP signaling. RNAi, in vitro and in vivo ubiquitination assays, Co-IP, Cul4b-deficient mouse embryonic fibroblasts Biochimica et biophysica acta High 23357576
2013 CUL4B promotes ubiquitination and degradation of TSC2 and cyclin E in neocortical neurons. XLID mutants (R388X, R572C, V745A) fail to promote ubiquitination/degradation of TSC2 or cyclin E. By targeting TSC2 for degradation, wild-type CUL4B positively regulates mTOR signaling (increased p-mTOR, p-p70S6K, p-4E-BP1); CUL4B knockdown or XLID mutants fail to activate mTOR. Adenovirus-mediated expression in neocortical neurons, shRNA knockdown, immunoblotting for ubiquitination and mTOR pathway substrates Biochimica et biophysica acta High 23348097
2015 CUL4B activates Wnt/β-catenin signaling in HCC by epigenetically silencing Wnt pathway antagonists (DKK1, PPP2R2B). CRL4B promotes recruitment/retention of PRC2 at promoters of Wnt antagonists, and CUL4B knockdown decreases PRC2 components and H3K27me3 at these promoters. CUL4B also protects β-catenin from GSK3-mediated degradation through this mechanism. RNAi knockdown, ChIP for PRC2/H3K27me3, rescue experiments with simultaneous knockdown of PPP2R2B, xenograft models The Journal of pathology High 25430888
2015 CRL4B is required for proteasomal degradation of HUWE1 in response to DNA damage. CUL4B is activated in a NEDD8-dependent manner and ubiquitinates HUWE1 in vitro and in vivo. CUL4B depletion stabilizes HUWE1, which accelerates MCL-1 degradation and increases apoptosis; these phenotypes are rescued by simultaneous HUWE1 depletion. In vitro ubiquitination assay, RNAi, epistasis (double knockdown rescue), immunoblotting Nucleic acids research High 25883150
2012 CUL4B is essential for embryonic development; Cul4b null mouse embryos die before E9.5 with severe developmental arrest. Cyclin E (a CRL4B substrate) accumulates in Cul4b null embryos. Epiblast-specific deletion of Cul4b prevents lethality, demonstrating that CUL4B's essential function is in extra-embryonic tissues rather than the embryo proper. Cul4b knockout mouse generation, conditional epiblast-specific deletion (Sox2-Cre), immunoblotting for cyclin E Cell research High 22453236 22606329
2016 CUL4B negatively regulates PPARγ by promoting its polyubiquitination and proteasomal degradation. Depletion of CUL4B leads to upregulation of PPARγ-regulated genes and facilitates adipogenesis. Adipocyte-specific Cul4b KO mice show increased adipogenesis and fat accumulation but improved insulin sensitivity and glucose tolerance. Adipocyte-specific conditional KO mouse, PPARγ ubiquitination assay, high-fat diet metabolic phenotyping Diabetes High 27899484
2016 CUL4B performs both cell-autonomous and non-cell-autonomous functions in spermatogenesis. In germ cells, CUL4B directly polyubiquitinates and degrades INSL6 (identified by mass spectrometry); loss of germ cell CUL4B impairs sperm motility due to reduced mitochondrial activity and glycolysis, and defective flagellar axoneme/outer dense fiber arrangement. In somatic cells, CUL4B maintains the spermatogonial stem cell niche. Germ cell-specific conditional KO, global KO, mass spectrometry substrate identification, polyubiquitination assay, sperm ATP measurement, electron microscopy of flagella The Journal of biological chemistry High 26846852
2015 CUL4B represses GFAP expression in neural progenitor cells by epigenetically silencing PTGDS. Loss of Cul4b increases PTGDS expression, elevating prostaglandin D2, which promotes astrocyte (GFAP+) generation. CUL4B/PRC2 complex targets and represses Ptgds; pharmacological inhibition of PTGDS or shRNA knockdown attenuates the increased GFAP+ cells from Cul4b-null NPCs. Nervous system-specific Cul4b KO mice, in vitro NPC culture, ChIP for CUL4B/PRC2 at Ptgds, pharmacological PTGDS inhibition, shRNA knockdown Human molecular genetics High 26025376
2015 CUL4B unexpectedly functions as a negative regulator of myeloid-derived suppressor cells (MDSCs). Hematopoietic-specific CUL4B ablation (Tek-Cre) increases MDSC accumulation via downregulation of the AKT/β-catenin pathway. CUL4B represses phosphatases PP2A and PHLPP1/2 (which dephosphorylate/inactivate AKT) to sustain AKT pathway activation. Hematopoietic-specific conditional KO (Tek-Cre), MDSC quantification, AKT pathway analysis, multiple tumor models Cancer research High 26450912
2019 CUL4B negatively regulates TLR-triggered inflammatory responses in macrophages by epigenetically repressing transcription of Pten, thereby maintaining the anti-inflammatory PI3K-AKT-GSK3β pathway. Deletion of CUL4B in macrophages upregulates PTEN, leading to increased GSK3β activity and excessive proinflammatory cytokine production. Myeloid cell-specific Cul4b KO mice, LPS/pathogen challenge, GSK3β inhibitor rescue, ChIP/epigenetic analysis of Pten promoter Cellular & molecular immunology High 31729464
2017 CUL4B epigenetically represses miR-194 by catalyzing monoubiquitination of H2AK119 and coordinating with PRC2 to promote H3K27me3 at the miR-194 gene cluster. miR-194 in turn targets the 3'-UTR of CUL4B, creating a double-negative feedback loop. CUL4B also represses miR-194 downstream of p53. ChIP for H2AK119ub and H3K27me3 at miR-194 locus, 3'-UTR luciferase reporter assay, RNAi, xenograft tumor formation Molecular oncology High 28164432
2021 CUL4B renders breast cancer cells tamoxifen-resistant through a miR-32-5p/ER-α36 axis. CRL4B epigenetically represses miR-32-5p transcription by catalyzing H2AK119 monoubiquitination and coordinating with PRC2 and HDAC complexes to promote H3K27me3 at the miR-32-5p promoter, leading to upregulation of ER-α36. ChIP for H2AK119ub and H3K27me3 at miR-32-5p promoter, dominant-negative CUL4B mutant, pharmacological inhibition of CRL4B/PRC2/HDAC, in vitro and in vivo tamoxifen sensitivity assays The Journal of pathology High 33638154
2014 CUL4B suppresses stress-induced cellular senescence by promoting p53 ubiquitination and proteasomal degradation in normal human fibroblasts exposed to oxidative stress (H2O2), thereby dampening a p53-ROS positive feedback loop that drives senescence. CUL4B is downregulated in stress-induced (but not replicative) senescent cells. Ectopic CUL4B expression, RNAi, H2O2 treatment, p53 ubiquitination assay, ROS measurement, senescence β-galactosidase assay Free radical biology & medicine High 25464270
2021 CUL4B promotes HBV replication by interacting with the viral protein HBx and inhibiting HBx ubiquitination and proteasomal degradation, thereby stabilizing HBx. This interaction was demonstrated by immunoprecipitation and immunofluorescence co-localization. Co-immunoprecipitation, immunofluorescence co-staining, cycloheximide chase assay, in vivo ubiquitination assay, Cul4b transgenic and conditional KO mice with hydrodynamic HBV model Cancer biology & medicine High 33969670
2021 TCDD-activated AHR is partially degraded via CUL4B-mediated ubiquitination; loss of CUL4B partially prevents AHR degradation after TCDD exposure. TiPARP additionally promotes AHR nuclear export preceding degradation; knockdown of TiPARP in CUL4B-null cells completely abolishes AHR degradation, indicating CUL4B and TiPARP cooperate to restrain AHR activity. Cul4b-null MEF cell line, TiPARP knockdown in null cells, TCDD treatment, AHR protein level and transcriptional activity measurement The Journal of biological chemistry High 34146543
2023 The unique N-terminal extension of CUL4B is heavily phosphorylated during mitosis. Phosphorylation triggers chromatin exclusion of CUL4B while promoting binding to actin regulators and to two previously unrecognized CUL4B-specific DCAFs, LIS1 and WDR1, which interact with DDB1 in a phosphorylation-dependent manner. CUL4B phosphorylation is required for efficient mitosis progression (spindle positioning, cortical tension). The XLID mutation CUL4B-P50L perturbs this phosphorylation pattern. CUL4B is required for developing stable ventricular structures in human forebrain organoids. Phosphoproteomic analysis, Co-immunoprecipitation of LIS1/WDR1 with DDB1, mutagenesis of phosphorylation sites, live imaging for spindle positioning/cortical tension, human forebrain organoid model The EMBO journal High 37365982
2023 CUL4B and DCAF7 form an E3 ligase complex that promotes MEN1 protein degradation by binding MEN1 and catalyzing its ubiquitination. Neddylation pathway activation is required upstream; neddylation inhibitor MLN4924 induces MEN1 accumulation. DCAF7 downregulation reverses everolimus resistance in PanNET cells in a MEN1-dependent manner. Co-IP, RNAi knockdown, neddylation inhibitor (MLN4924), in vitro ubiquitination, DCAF7/MEN1 double knockdown epistasis Cancer research High 36939378
2023 CUL4B directly binds to the promoter of Cxcl2 and epigenetically represses its transcription. In KRAS-mutant lung tumors, CUL4B deletion increases CXCL2 expression, which promotes CXCR2-dependent MDSC recruitment to the tumor microenvironment. ChIP for CUL4B at Cxcl2 promoter, CUL4B KO/KD in autochthonous and transplantable KRAS-mutant tumor models, MDSC quantification, MDSC targeting experiments Oncogene High 37653114
2024 CUL4B mutations (XLID patient-derived) cause premature cell cycle exit and precocious neuronal differentiation of neural progenitor cells, as well as increased synapse formation and enhanced neuronal excitability. Mechanistically, CRL4B complex represses transcription of PPP2R2B and PPP2R2C (PP2A regulatory subunit isoforms) by catalyzing H2AK119 monoubiquitination at their promoters. CUL4B mutations upregulate PP2A activity, inhibiting AKT and ERK, causing premature cell cycle exit; rescue achieved by AKT/ERK activation or PP2A inhibition. Patient iPSC-derived 2D neuronal cultures and cerebral organoids, ChIP for H2AK119ub, PP2A activity assay, AKT/ERK inhibition/activation, pharmacological PP2A inhibition rescue Cell death & disease High 38331954
2021 CUL4B is required for CD4+ T cell proliferation and survival following TCR stimulation. In T cells, CUL4B preferentially associates with the substrate receptor DCAF1; Cul4b and DCAF1 interact with proteins involved in DNA damage sensing/repair. Cul4b-deficient CD4+ T cells accumulate DNA damage and fail to phosphorylate SMC1A (a downstream DNA damage response event), leading to proliferative failure. Cul4b conditional KO T cells, Co-immunoprecipitation (CUL4B-DCAF1 interaction), mass spectrometry of associated proteins, DNA damage assays, SMC1A phosphorylation analysis PLoS biology High 33524014
2023 c-Myc increases Cul4b protein levels following T cell activation. Cul4b-deficient CD8+ T cells accumulate DNA damage and p21/Cyclin E2, leading to replication stress and proliferative catastrophe. c-Myc supports CD8+ T cell expansion by maintaining genome stability via Cul4b. Cul4b conditional KO CD8+ T cells, in vivo LCMV infection model, p21/Cyclin E2 immunoblotting, DNA damage assays Nature communications High 37925424
2015 CUL4B variants interact with WDR62, a protein mutated in microcephaly patients with malformations of cortical development, suggesting a functional complex. Co-immunoprecipitation of CUL4B with WDR62 Human mutation Low 25385192
2019 CUL4B epigenetically represses IL-6 transcription in myeloid cells via the CRL4B complex. In CUL4B-deficient MDSCs, elevated IL-6 activates IL-6/STAT3 signaling in tumor cells, rendering them stem cell-like properties. Hematopoietic/myeloid-specific Cul4b KO mice, IL-6 blocking, STAT3 inhibitor, ChIP/epigenetic analysis at IL-6 promoter Oncogene High 31235785
2012 CUL4B is required for neural progenitor cell mitosis progression. Downregulation of CUL4B arrests NPCs and NT-2 cells in G2/M phase. Three isoforms of CUL4B exist in brain tissue; the larger isoforms (CUL4B-1, -2) are predominantly unneddylated due to their N-terminal extension, while the smaller isoform (CUL4B-3, lacking the N-terminus) is neddylated. Unneddylated CUL4B accumulates during mitosis and inhibits β-catenin degradation in neural stem cell niches. Immunostaining, RNAi knockdown in NPCs/NT-2 cells, cell cycle analysis, neddylation state analysis, brain tissue immunostaining BMC neuroscience Medium 22992378
2023 CUL4B-DDB1-COP1 forms a functional E3 ubiquitin ligase complex responsible for targeting the histone demethylase UTX (KDM6A) for ubiquitination and proteasomal degradation in colorectal cancer cells. Cop1 deficiency in mouse intestinal tissue results in UTX accumulation and restricts tumorigenesis. Co-immunoprecipitation demonstrating CUL4B-DDB1-COP1 complex, immunoblot for UTX degradation, conditional Cop1 KO mouse, spontaneous AOM/DSS CRC model with GSK126 rescue Experimental hematology & oncology High 37679762
2023 CUL4B promotes osteogenesis and inhibits adipogenesis of mesenchymal stem cells by epigenetically repressing KLF4 (an adipogenic factor) and C/EBPδ transcription. The CUL4B complex directly binds to Klf4 and Cebpd promoters and represses their transcription. MSC-specific Cul4b KO mice show impaired skeletal development, low bone mass, and increased marrow adipose accumulation. MSC-specific conditional Cul4b KO mice, ChIP for CUL4B complex at Klf4/Cebpd promoters, in vitro osteogenic/adipogenic differentiation assays, ovariectomy model Bone research High 37268647
2019 CUL4B-RING E3 ligase complex represses transcription of miR-372/373 via H2AK119 monoubiquitination at the miR-372/373 gene cluster, leading to upregulation of PIK3CA and activation of AKT signaling, promoting bladder cancer metastasis and stemness. ChIP for H2AK119ub at miR-372/373 locus, 3'-UTR reporter assay for PIK3CA targeting, RNAi, invasion/motility assays Oncogene High 32127645
2024 Prior to radiation exposure, CUL4B inhibits ubiquitination of PSME3, leading to PSME3 accumulation that negatively regulates p53-mediated apoptosis. After radiation, CUL4B dissociates from PSME3 and translocates to γH2AX foci in the nucleus, where it impedes DNA damage repair by inhibiting BRCA1 phosphorylation and RAD51, augmenting p53-mediated apoptosis. In vivo and in vitro intestinal models, co-IP for CUL4B-PSME3 interaction, BRCA1 phosphorylation and RAD51 immunoblotting, γH2AX co-localization by immunofluorescence Scientific reports Medium 38689033
2024 CUL4B protects kidneys from acute injury by promoting polyubiquitination and proteasomal degradation of p53, thereby suppressing p53-dependent PAI-1 expression. CUL4B-deficient kidneys show exacerbated apoptosis and inflammation upon cisplatin or ischemia-reperfusion injury; inhibition of PAI-1 or p53 prevents this aggravation. Kidney-specific Cul4b KO mice, cisplatin and IR injury models, p53 ubiquitination assay, PAI-1 and p53 inhibition rescue experiments, transcriptome analysis Cell death & disease High 39695153
2019 CUL4B in macrophages represses miR-194-5p expression; loss of CUL4B in macrophages suppresses their migration, adhesion, and renal infiltration by elevating miR-194-5p, which targets and reduces integrin α9 (ITGA9). High glucose upregulates CUL4B in macrophages in diabetic conditions. Myeloid-specific Cul4b KO mice with diabetic kidney disease models, miR-194-5p/ITGA9 pathway analysis, macrophage migration/adhesion assays Cell reports High 37224018
2019 Small molecule TSC01682 specifically disrupts the CUL4B-DDB1 protein-protein interaction, preventing CRL4B complex assembly. TSC01682 treatment decreases CRL4B components DCAF11 and DCAF13, and increases the CRL4B substrates p21 and PTEN by inhibiting their ubiquitination, consistent with CUL4B-DDB1 interaction being required for substrate ubiquitination. In vitro high-throughput screening (yeast), Co-IP in osteosarcoma cells, p21/PTEN ubiquitination assay, xenograft tumor model American journal of cancer research High 31598391
2020 CUL4B contributes to colorectal cancer stemness by coordinating with PRC2 to epigenetically repress miR34a expression, leading to upregulation of MYCN and NOTCH1 (miR34a targets). Inhibition of CUL4B in patient-derived tumor organoids reduces sphere formation and metastatic capacity. RNAi in cell lines and patient-derived organoids, ChIP for CUL4B/PRC2 at miR34a locus, sphere formation assays Oncogenesis High 32054830
2021 CUL4B promotes temozolomide resistance in glioblastoma by coordinating with HDAC to co-occupy the CDKN1A (p21) promoter and epigenetically silence CDKN1A transcription, thereby attenuating TMZ-induced cellular senescence. ChIP demonstrating CUL4B and HDAC co-occupancy at CDKN1A promoter, senescence β-galactosidase assay, CUL4B KD/OE in GBM cells Frontiers in oncology High 33869025
2025 CUL4B drives thyroid cancer dedifferentiation by promoting ubiquitination of ARID1A (a SWI/SNF complex subunit), leading to its degradation and decreased expression of the differentiation marker PAX8. In vivo TC models, RNA-seq, Co-IP and ubiquitination assay for CUL4B-ARID1A, immunohistochemistry, in vitro functional experiments Translational oncology High 40203790
2021 THAP7-AS1 lncRNA interacts with the N-terminal 1-50 amino acid region (NLS) of CUL4B and promotes the interaction between the NLS and importin α1, thereby facilitating CUL4B nuclear import. Nuclear CUL4B then represses miR-22-3p and miR-320a by catalyzing H2AK119ub1 and coordinating EZH2-mediated H3K27me3. Co-IP/RIP for THAP7-AS1-CUL4B interaction, NLS domain mapping, importin α1 interaction assay, ChIP for H2AK119ub1 and H3K27me3 Cell death and differentiation Medium 34608273
2016 CUL4B promotes invasion and metastasis of gastric cancer by epigenetically repressing miR-125a expression, leading to HER2 upregulation and subsequent activation of PI3K/AKT signaling. HER2 inhibitors reverse CUL4B-induced EMT. ChIP for epigenetic marks at miR-125a locus, HER2 inhibitor treatment, gain- and loss-of-function experiments, xenograft metastasis model Oncogene Medium 29106389
2023 CUL4B directly interacts with the promoter of Csf3 (encoding G-CSF) by coordinating with PRC2, epigenetically repressing G-CSF expression. CUL4B deletion in intestinal epithelium epigenetically activates G-CSF, promoting MDSC recruitment and ApcMin/+ adenoma formation. ChIP for CUL4B/PRC2 at Csf3 promoter, ApcMin/+;Cul4bΔIEC mouse model, MDSC depletion rescue, organoid co-culture with MDSCs Neoplasia High 38761506

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor. American journal of human genetics 195 17236139
2021 lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus. Cell death and differentiation 163 34608273
2007 Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation. American journal of human genetics 135 17273978
2009 Characterization of nuclear localization signal in the N terminus of CUL4B and its essential role in cyclin E degradation and cell cycle progression. The Journal of biological chemistry 105 19801544
2015 Distinct and overlapping functions of the cullin E3 ligase scaffolding proteins CUL4A and CUL4B. Gene 99 26344709
2012 Lack of Cul4b, an E3 ubiquitin ligase component, leads to embryonic lethality and abnormal placental development. PloS one 70 22606329
2015 CUL4B activates Wnt/β-catenin signalling in hepatocellular carcinoma by repressing Wnt antagonists. The Journal of pathology 65 25430888
2012 Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis. Cell research 60 22453236
2019 Circular RNA ZFR accelerates non-small cell lung cancer progression by acting as a miR-101-3p sponge to enhance CUL4B expression. Artificial cells, nanomedicine, and biotechnology 58 31407591
2015 The CUL4B/AKT/β-Catenin Axis Restricts the Accumulation of Myeloid-Derived Suppressor Cells to Prohibit the Establishment of a Tumor-Permissive Microenvironment. Cancer research 51 26450912
2012 Rescue of the genetically engineered Cul4b mutant mouse as a potential model for human X-linked mental retardation. Human molecular genetics 50 22763239
2009 A novel nonsense mutation in CUL4B gene in three brothers with X-linked mental retardation syndrome. Clinical genetics 49 20002452
2016 Lack of CUL4B in Adipocytes Promotes PPARγ-Mediated Adipose Tissue Expansion and Insulin Sensitivity. Diabetes 47 27899484
2010 Deletion of the CUL4B gene in a boy with mental retardation, minor facial anomalies, short stature, hypogonadism, and ataxia. American journal of medical genetics. Part A 43 20014135
2019 Inflammation-dependent downregulation of miR-194-5p contributes to human intervertebral disc degeneration by targeting CUL4A and CUL4B. Journal of cellular physiology 41 30945295
2017 CUL4B promotes gastric cancer invasion and metastasis-involvement of upregulation of HER2. Oncogene 39 29106389
2015 Variants in CUL4B are associated with cerebral malformations. Human mutation 39 25385192
2017 MicroRNA-300 promotes apoptosis and inhibits proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway by targeting CUL4B in pancreatic cancer cells. Journal of cellular biochemistry 38 28685847
2016 Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer. The Journal of biological chemistry 37 27974468
2011 CUL4B-deficiency in humans: understanding the clinical consequences of impaired Cullin 4-RING E3 ubiquitin ligase function. Mechanisms of ageing and development 37 21352845
2023 MEN1 Degradation Induced by Neddylation and the CUL4B-DCAF7 Axis Promotes Pancreatic Neuroendocrine Tumor Progression. Cancer research 36 36939378
2019 NCBP1 promotes the development of lung adenocarcinoma through up-regulation of CUL4B. Journal of cellular and molecular medicine 36 31448526
2017 Dysregulation of the miR-194-CUL4B negative feedback loop drives tumorigenesis in non-small-cell lung carcinoma. Molecular oncology 36 28164432
2021 The Traditional Chinese Medicine Compound Huangqin Qingre Chubi Capsule Inhibits the Pathogenesis of Rheumatoid Arthritis Through the CUL4B/Wnt Pathway. Frontiers in pharmacology 34 34512369
2016 Cell Autonomous and Nonautonomous Function of CUL4B in Mouse Spermatogenesis. The Journal of biological chemistry 34 26846852
2015 DNA damage-induced activation of CUL4B targets HUWE1 for proteasomal degradation. Nucleic acids research 33 25883150
2020 The CUL4B-miR-372/373-PIK3CA-AKT axis regulates metastasis in bladder cancer. Oncogene 32 32127645
2021 Roles of the ubiquitin ligase CUL4B and ADP-ribosyltransferase TiPARP in TCDD-induced nuclear export and proteasomal degradation of the transcription factor AHR. The Journal of biological chemistry 31 34146543
2013 CUL4B promotes replication licensing by up-regulating the CDK2-CDC6 cascade. The Journal of cell biology 30 23479742
2020 Downregulation of lncRNA ZEB1-AS1 Represses Cell Proliferation, Migration, and Invasion Through Mediating PI3K/AKT/mTOR Signaling by miR-342-3p/CUL4B Axis in Prostate Cancer. Cancer biotherapy & radiopharmaceuticals 29 32275162
2018 miR‑381 and miR‑489 suppress cell proliferation and invasion by targeting CUL4B via the Wnt/β‑catenin pathway in gastric cancer. International journal of oncology 29 30483755
2014 CUL4B promotes proliferation and inhibits apoptosis of human osteosarcoma cells. Oncology reports 29 25189186
2019 CUL4B/miR-33b/C-MYC axis promotes prostate cancer progression. The Prostate 28 30609075
2016 Human X-linked Intellectual Disability Factor CUL4B Is Required for Post-meiotic Sperm Development and Male Fertility. Scientific reports 28 26832838
2023 CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental hematology & oncology 27 37679762
2021 CUL4B renders breast cancer cells tamoxifen-resistant via miR-32-5p/ER-α36 axis. The Journal of pathology 27 33638154
2020 Cul4B promotes the progression of ovarian cancer by upregulating the expression of CDK2 and CyclinD1. Journal of ovarian research 27 32622365
2017 CUL4B promotes bladder cancer metastasis and induces epithelial-to-mesenchymal transition by activating the Wnt/β-catenin signaling pathway. Oncotarget 27 29100384
2016 Knockdown of CUL4B Suppresses the Proliferation and Invasion in Non-Small Cell Lung Cancer Cells. Oncology research 27 27656838
2021 CUL4B Promotes Breast Carcinogenesis by Coordinating with Transcriptional Repressor Complexes in Response to Hypoxia Signaling Pathway. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 26 34026424
2020 Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway. Cancer cell international 26 33292255
2019 CUL4B promotes prostate cancer progression by forming positive feedback loop with SOX4. Oncogenesis 26 30872583
2019 Upregulation of IL-6 in CUL4B-deficient myeloid-derived suppressive cells increases the aggressiveness of cancer cells. Oncogene 26 31235785
2014 CUL4B impedes stress-induced cellular senescence by dampening a p53-reactive oxygen species positive feedback loop. Free radical biology & medicine 25 25464270
2020 CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis 24 32054830
2023 Depletion of CUL4B in macrophages ameliorates diabetic kidney disease via miR-194-5p/ITGA9 axis. Cell reports 23 37224018
2019 CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription. Cellular & molecular immunology 23 31729464
2018 CUL4B promotes the pathology of adjuvant-induced arthritis in rats through the canonical Wnt signaling. Journal of molecular medicine (Berlin, Germany) 23 29626254
2019 CUL4B regulates autophagy via JNK signaling in diffuse large B-cell lymphoma. Cell cycle (Georgetown, Tex.) 20 30612524
2018 CUL4B promotes metastasis and proliferation in pancreatic cancer cells by inducing epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway. Journal of cellular biochemistry 20 29274277
2015 Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain. Human molecular genetics 20 26025376
2013 X-linked intellectual disability gene CUL4B targets Jab1/CSN5 for degradation and regulates bone morphogenetic protein signaling. Biochimica et biophysica acta 20 23357576
2012 Cul4B regulates neural progenitor cell growth. BMC neuroscience 20 22992378
2021 The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA. PLoS biology 19 33524014
2018 MiR-431 inhibits colorectal cancer cell invasion via repressing CUL4B. European review for medical and pharmacological sciences 19 29863249
2015 Decreased CUL4B expression inhibits malignant proliferation of glioma in vitro and in vivo. European review for medical and pharmacological sciences 19 25855927
2015 Knockdown of CUL4B inhibits proliferation and promotes apoptosis of colorectal cancer cells through suppressing the Wnt/β-catenin signaling pathway. International journal of clinical and experimental pathology 19 26617747
2020 Circ_0074027 Contributes to Non-Small Cell Lung Cancer Progression by Upregulating CUL4B Expression Through miR-335-5p. Cancer biotherapy & radiopharmaceuticals 18 32580576
2018 MiRNA-708/CUL4B axis contributes into cell proliferation and apoptosis of osteosarcoma. European review for medical and pharmacological sciences 17 30229816
2023 The CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs. The EMBO journal 16 37365982
2021 Inhibition of MicroRNA miR-101-3p on prostate cancer progression by regulating Cullin 4B (CUL4B) and PI3K/AKT/mTOR signaling pathways. Bioengineered 16 34338146
2013 XLID CUL4B mutants are defective in promoting TSC2 degradation and positively regulating mTOR signaling in neocortical neurons. Biochimica et biophysica acta 16 23348097
2014 Donor splice-site mutation in CUL4B is likely cause of X-linked intellectual disability. American journal of medical genetics. Part A 15 24898194
2021 LncRNA SNHG12 regulates the miR-101-3p/CUL4B axis to mediate the proliferation, migration and invasion of non-small cell lung cancer. The Kaohsiung journal of medical sciences 14 34002487
2014 Zebrafish cul4a, but not cul4b, modulates cardiac and forelimb development by upregulating tbx5a expression. Human molecular genetics 14 25274780
2015 CUL4B: a novel epigenetic driver in Wnt/β-catenin-dependent hepatocarcinogenesis. The Journal of pathology 13 25664533
2024 CUL4B mutations impair human cortical neurogenesis through PP2A-dependent inhibition of AKT and ERK. Cell death & disease 12 38331954
2019 Alu-mediated Xq24 deletion encompassing CUL4B, LAMP2, ATP1B4, TMEM255A, and ZBTB33 genes causes Danon disease in a female patient. American journal of medical genetics. Part A 12 31729179
2023 Knockdown of circMFN2 inhibits cell progression and glycolysis by miR-198/CUL4B pathway in ovarian cancer. Journal of biochemical and molecular toxicology 11 37158446
2023 CUL4B orchestrates mesenchymal stem cell commitment by epigenetically repressing KLF4 and C/EBPδ. Bone research 11 37268647
2020 CREB acts as a common transcription factor for major epigenetic repressors; DNMT3B, EZH2, CUL4B and E2F6. Medical oncology (Northwood, London, England) 11 32710193
2019 CUL4B regulates cancer stem-like traits of prostate cancer cells by targeting BMI1 via miR200b/c. The Prostate 11 31111526
2019 Small molecule TSC01682 inhibits osteosarcoma cell growth by specifically disrupting the CUL4B-DDB1 interaction and decreasing the ubiquitination of CRL4B E3 ligase substrates. American journal of cancer research 11 31598391
2017 Genome-first approach diagnosed Cabezas syndrome via novel CUL4B mutation detection. Human genome variation 11 28144446
2022 LncRNA NEAT1 Targets miR-342-3p/CUL4B to Inhibit the Proliferation of Cutaneous Squamous Cell Carcinoma Cells. Journal of oncology 10 35909905
2023 CUL4B functions as a tumor suppressor in KRAS-driven lung tumors by inhibiting the recruitment of myeloid-derived suppressor cells. Oncogene 9 37653114
2023 c-Myc uses Cul4b to preserve genome integrity and promote antiviral CD8+ T cell immunity. Nature communications 9 37925424
2022 Exosomal circKDM4A Induces CUL4B to Promote Prostate Cancer Cell Malignancy in a miR-338-3p-Dependent Manner. Biochemical genetics 9 35930171
2022 CUL4B increases platinum-based drug resistance in colorectal cancer through EMT: A study in its mechanism. Journal of cellular and molecular medicine 9 36385733
2022 CUL4B Upregulates RUNX2 to Promote the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Epigenetically Repressing the Expression of miR-320c and miR-372/373-3p. Frontiers in cell and developmental biology 8 35784474
2020 Genome-first approach for the characterization of a complex phenotype with combined NBAS and CUL4B deficiency. Bone 8 32768688
2016 CUL4B, NEDD4, and UGT1As involve in the TGF-β signalling in hepatocellular carcinoma. Annals of hepatology 8 27236156
2024 CUL4B protects kidneys from acute injury by restraining p53/PAI-1 signaling. Cell death & disease 7 39695153
2022 Lnc-TC/miR-142-5p/CUL4B signaling axis promoted cell ferroptosis to participate in benzene hematotoxicity. Life sciences 7 36272464
2022 CUL4B-associated epilepsy: Report of a novel truncating variant promoting drug-resistant seizures and systematic review of the literature. Seizure 7 36476360
2021 Effect and mechanism of miR-217 on drug resistance, invasion and metastasis of ovarian cancer cells through a regulatory axis of CUL4B gene silencing/inhibited Wnt/β-catenin signaling pathway activation. European review for medical and pharmacological sciences 6 33506897
2024 Circ_0011058 alleviates RA pathology through the circ_0011058/miR-335-5p/CUL4B signal axis. Autoimmunity 5 38254314
2024 Enhanced ApcMin/+ adenoma formation after epithelial CUL4B deletion by recruitment of myeloid-derived suppressor cells. Neoplasia (New York, N.Y.) 5 38761506
2021 CUL4B Promotes Temozolomide Resistance in Gliomas by Epigenetically Repressing CDNK1A Transcription. Frontiers in oncology 5 33869025
2021 CUL4B facilitates HBV replication by promoting HBx stabilization. Cancer biology & medicine 5 33969670
2019 CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β-catenin signaling pathway. Cancer medicine 5 30883036
2024 Dynamic role of CUL4B in radiation-induced intestinal injury-regeneration. Scientific reports 4 38689033
2023 CUL4B enhances the malignant phenotype of esophageal squamous cell carcinoma by suppressing TGFBR3 expression. Biochemical and biophysical research communications 4 37487438
2023 B cell expression of E3 ubiquitin ligase Cul4b promotes chronic gammaherpesvirus infection in vivo. Journal of virology 4 37962378
2023 Histone ubiquitination-related gene CUL4B promotes lung adenocarcinoma progression and cisplatin resistance. Frontiers in genetics 4 38075690
2020 CUL4B promotes aggressive phenotypes of renal cell carcinoma via upregulating c-Met expression. The international journal of biochemistry & cell biology 4 33227394
2025 NSUN2 promotes colorectal cancer progression and increases lapatinib sensitivity by enhancing CUL4B/ErbB-STAT3 signalling in a non-m5C manner. Clinical and translational medicine 3 40156167
2025 CUL4B regulates thyroid cancer differentiation and treatment sensitivity by ubiquitinating ARID1A. Translational oncology 3 40203790
2025 Circ_0003520/miR-205-5p/CUL4B Axis Drives the Progression of Clear Cell Renal Carcinoma. Journal of biochemical and molecular toxicology 3 40320861
2019 Embryonic Cul4b is important for epiblast growth and location of primitive streak layer cells. PloS one 3 31260508

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