Affinage

RAB3A

Ras-related protein Rab-3A · UniProt P20336

Length
220 aa
Mass
25.0 kDa
Annotated
2026-04-28
100 papers in source corpus 60 papers cited in narrative 58 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB3A is a small GTPase that cycles between GTP-bound (membrane-associated, active) and GDP-bound (cytosolic, inactive) states to regulate the docking, priming, and Ca²⁺-dependent fusion of secretory vesicles at specialized release sites in neurons, neuroendocrine cells, and non-neuronal cell types. Geranylgeranylation of its C-terminal Cys-X-Cys motif anchors RAB3A to vesicle membranes, where its GTPase cycle — driven by the GEF GRAB, GAP proteins (including TBC-domain family members), and GDI — controls the recruitment of effectors including rabphilin-3A, RIM1α, Munc13-1, synapsin I, Slp4-a, NMHC IIA, and myosin-Va to coordinate vesicle transport, active-zone docking, and readily releasable pool replenishment (PMID:1648935, PMID:11516400, PMID:10025402, PMID:15265868, PMID:27325790, PMID:21349835). Loss of RAB3A abolishes mossy fiber LTP, impairs activity-dependent vesicle recruitment to active zones, reduces first-phase insulin secretion from pancreatic β-cells, and compromises plasma membrane repair via lysosome exocytosis (PMID:9252190, PMID:11598194, PMID:12510060, PMID:27325790). Beyond canonical synaptic exocytosis, RAB3A functions in acrosomal exocytosis in spermatozoa — acting upstream of NSF in a Ca²⁺-dependent docking/fusion cascade — and participates in APP transport vesicle assembly and BDNF vesicle docking in astrocytes (PMID:10727281, PMID:10954749, PMID:19923287, PMID:27559163).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1990 High

    Establishing that RAB3A is a membrane-associated secretory vesicle protein resolved its subcellular site of action, linking it to the regulated exocytosis machinery rather than constitutive trafficking.

    Evidence Subcellular fractionation and immunoadsorption of chromaffin granules, followed by immunofluorescence at frog motor end plates showing translocation during exocytosis

    PMID:1655810 PMID:2165599

    Open questions at the time
    • Mechanism of initial targeting to secretory vesicles (vs. Golgi or endosomes) not determined
    • Whether association is direct lipid-mediated or protein-mediated was unclear
  2. 1991 High

    Identifying geranylgeranylation of the C-terminal Cys-X-Cys as the membrane anchor explained how RAB3A partitions between cytosol and vesicle membranes and established the lipid modification as functionally required.

    Evidence Mevalonate pathway inhibition (compactin) and C-terminal cysteine mutagenesis abolishing membrane binding; later confirmed by mass spectrometry of dual geranylgeranylation

    PMID:1648935 PMID:7991565

    Open questions at the time
    • How geranylgeranylated RAB3A is specifically sorted to synaptic/secretory vesicles rather than other endomembranes was not resolved
  3. 1992 High

    Demonstrating that effector-domain peptides stimulate exocytosis in mast cells, pancreatic acini, and β-cells established that the GTP-bound effector interface is the functional surface driving fusion, and identified the first putative effector (p85/rabphilin-3A).

    Evidence Patch-clamp capacitance in mast cells, amylase release from permeabilized acini, and chemical cross-linking identifying an 85-kDa GTP-dependent target

    PMID:1331813 PMID:1371881 PMID:1597436

    Open questions at the time
    • Identity of the cross-linked target only later confirmed as rabphilin-3A
    • Whether peptide effects faithfully recapitulate intact protein function was debated
  4. 1993 High

    Characterization of rabphilin-3A as a bona fide GTP-dependent effector with dual C2 domains, and mapping of the complete GTPase regulatory cycle (GDI, GEF/GRF, GAP), provided the first integrated biochemical framework for RAB3A signaling on vesicles.

    Evidence Cloning and domain analysis of rabphilin-3A showing GTP-selective binding and GAP inhibition; nucleotide-state analysis in PC12 cell fractions defining GDI/GRF/GAP interplay

    PMID:8226729 PMID:8262955 PMID:8384302 PMID:8387493

    Open questions at the time
    • In vivo relevance of rabphilin-3A for neurotransmitter release not yet tested genetically
    • Molecular identity of the brain GEF (GRF) remained unknown
  5. 1994 High

    RAB3A knockout mice revealed that the protein is dispensable for basal single-stimulus release but required for vesicle recruitment during repetitive activity and for maintaining rabphilin-3A at synapses, shifting the model from a simple fusion trigger to a regulator of vesicle supply and Ca²⁺ sensitivity.

    Evidence Homologous recombination knockout with hippocampal electrophysiology showing enhanced synaptic depression; overexpression of Q81L mutant inhibiting secretion in chromaffin cells; nucleotide-state shift during α-latrotoxin-induced exocytosis

    PMID:7911226 PMID:7929154 PMID:7946335 PMID:8144603

    Open questions at the time
    • Redundancy with Rab3B/C/D complicated interpretation of single KO
    • Whether the 'inhibitory brake' model or 'recruitment facilitator' model was primary remained contentious
  6. 1997 High

    Electrophysiological dissection in RAB3A-null mice demonstrated that RAB3A acts at a late post-docking step to regulate the probability of Ca²⁺-triggered fusion, and is absolutely required for mossy fiber LTP, establishing a presynaptic plasticity function.

    Evidence Readily releasable pool measurements showing normal pool size but altered release probability in KO; abolished mossy fiber LTP with normal short-term plasticity

    PMID:9194562 PMID:9252190

    Open questions at the time
    • Molecular mechanism linking RAB3A to LTP expression unknown
    • Whether Ca²⁺/calmodulin-dependent membrane dissociation (demonstrated in vitro) contributes in vivo was untested
  7. 1999 High

    The crystal structure of GTP-Rab3A bound to the rabphilin-3A effector domain revealed a dual-interface recognition mechanism including a Rab-specific complementarity-determining region (RabCDR), explaining how RAB3A achieves effector selectivity.

    Evidence X-ray crystallography at 2.6 Å resolution

    PMID:10025402

    Open questions at the time
    • Structure of RAB3A with other effectors (RIM, Slp4-a) not determined
    • Conformational dynamics during the GTPase cycle not captured
  8. 2001 High

    Identification of GRAB as the physiological GEF for RAB3A, and EM demonstration that RAB3A is required for activity-dependent vesicle accumulation at active zones, established the upstream activation mechanism and refined the docking phenotype.

    Evidence GRAB catalyzes GDP→GTP exchange on RAB3A and regulates dopamine/GH release; EM of RAB3A-null terminals shows abolished depolarization-induced vesicle recruitment to active zones

    PMID:11516400 PMID:11598194

    Open questions at the time
    • How GRAB itself is regulated spatiotemporally at the synapse was unknown
    • Whether GRAB is the sole RAB3A GEF in all cell types was not established
  9. 2003 High

    Systematic mapping of RIM1/RIM2 interactions with Rab3 isoforms, identification of rabconnectin-3 as a scaffold coordinating GEF/GAP, and demonstration that RAB3A loss impairs first-phase insulin secretion broadened the effector network and extended RAB3A function to endocrine physiology.

    Evidence Mutagenesis/chimeric analysis of RIM Rab-binding domain; co-IP of rabconnectin-3 subunits with GEP/GAP; glucose tolerance and islet insulin secretion in RAB3A-null mice

    PMID:12510060 PMID:12578829 PMID:12786944

    Open questions at the time
    • Functional redundancy between RIM1α and RIM2 in RAB3A-dependent processes unclear
    • Whether rabconnectin-3 is present at all RAB3A-positive vesicle populations was untested
  10. 2006 High

    TIRF imaging and epistasis experiments established that RAB3A and RAB27A cooperatively promote dense-core vesicle docking, and that RAB3A-effector RIM1α recruits Munc13 to active zones, linking RAB3A to the vesicle priming machinery.

    Evidence siRNA double-knockdown reducing docking more than single KD in PC12 cells; RIM1α KO reducing Munc13-1 active-zone levels; Munc13 point mutant abolishing RIM binding

    PMID:16684812 PMID:16704978

    Open questions at the time
    • Direct RAB3A–Munc13 interaction not demonstrated
    • Whether RAB3A and RAB27A act on the same or distinct vesicle pools was unresolved
  11. 2007 High

    Demonstrating that GTP hydrolysis cycling (not a locked nucleotide state) is required for RAB3A's docking function, and that this function depends on Munc18-1, placed RAB3A within a defined epistatic docking cascade (RAB3A cycling → Munc18-1 → docking).

    Evidence Both GTP- and GDP-locked RAB3A mutants fail to promote docking; wild-type RAB3A cannot rescue docking in munc18-1-null chromaffin cells

    PMID:17637832 PMID:17640821

    Open questions at the time
    • How Munc18-1 reads the RAB3A nucleotide state mechanistically was unknown
    • Whether this epistatic hierarchy applies at all synapse types was not tested
  12. 2011 High

    Identification of myosin-Va as a direct GTP-dependent RAB3A effector on synaptic vesicles linked RAB3A to actin-based vesicle transport, and coupling of the RAB3A/RIM/Munc13-1/Munc18-1 cascade integrated docking with priming.

    Evidence Analytical ultracentrifugation and GST pulldown demonstrating direct Myo5a–GTP-Rab3A interaction; epistasis experiments with Munc13-1/Munc18-1 co-expression in neuroendocrine cells

    PMID:21349835 PMID:21689256

    Open questions at the time
    • Whether Myo5a-RAB3A interaction is required for long-range vesicle transport versus local docking movements was unclear
    • Structural basis of the Myo5a–RAB3A interface unknown
  13. 2012 High

    At ribbon synapses, RAB3A was shown to deliver vesicles to Ca²⁺-dependent release sites in a GTP-hydrolysis-dependent manner, and in sperm the RIM/Munc13/RAB3A module was confirmed to mediate acrosomal membrane docking, generalizing the RAB3A docking mechanism beyond conventional synapses.

    Evidence Fluorescent RAB3A delivery via patch pipette with GTPase-deficient mutant blocking resupply; antibody/recombinant protein sequestration impairing acrosomal docking by EM

    PMID:22248876 PMID:22593061

    Open questions at the time
    • Ribbon-specific effectors beyond RIM not identified
    • Whether the sperm acrosomal mechanism requires Munc18 homologs was untested
  14. 2016 High

    Discovery that RAB3A functions on lysosomes — recruiting Slp4-a and NMHC IIA to position lysosomes at the cell periphery for exocytosis and plasma membrane repair — extended RAB3A beyond secretory granule biology into a broader membrane repair pathway.

    Evidence siRNA screen of Rab family followed by mechanistic dissection: RAB3A silencing impairs lysosome positioning, PMR assay (streptolysin-O), co-IP identifying NMHC IIA as effector

    PMID:27325790

    Open questions at the time
    • Whether RAB3A's lysosomal function uses the same GEF (GRAB) and GAP as synaptic vesicles was unknown
    • Relative contribution of RAB3A versus other Rabs (e.g., RAB27A) to PMR not quantified
  15. 2021 Medium

    Placement of RAB3A downstream of a Rab11→GRAB cascade for Ca²⁺-induced lysosome exocytosis revealed how RAB3A activation is coordinated by inter-Rab signaling outside the neuronal context.

    Evidence siRNA silencing of Rab11a/b, live imaging of Rab11–lysosome interactions, co-IP of Rab11 with GRAB and RAB3A

    PMID:34100549

    Open questions at the time
    • Whether Rab11→GRAB→RAB3A cascade operates at synapses is unknown
    • Direct demonstration that Rab11 activates GRAB enzymatic activity is lacking
    • Single-lab finding not independently confirmed

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include how RAB3A achieves vesicle-type-specific targeting despite ubiquitous geranylgeranylation, the structural basis of RAB3A interaction with RIM, Slp4-a, and Myo5a, and the precise mechanism by which RAB3A cycling controls mossy fiber LTP expression.
  • No structure of RAB3A in complex with RIM or Slp4-a
  • Mechanism connecting RAB3A to LTP expression remains molecularly undefined
  • Relative contributions of individual RAB3A effectors to docking versus priming versus fusion not dissected in a single system

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 4 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0031410 cytoplasmic vesicle 5 GO:0005886 plasma membrane 3 GO:0005764 lysosome 2 GO:0005829 cytosol 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 8 R-HSA-112316 Neuronal System 5 R-HSA-1474165 Reproduction 3 R-HSA-162582 Signal Transduction 3
Partners
GRABMYH9MYO5ARIMS1RIMS2RPH3ASYN1SYTL4
Complex memberships
RAB3A–RIM1α–Munc13-1RAB3A–Slp4-a–NMHC IIARAB3A–rabphilin-3A

Evidence

Reading pass · 58 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 Rab3A associates with synaptic vesicle membranes at the cell periphery (not at the Golgi), and undergoes translocation to the cell surface during massive exocytosis, suggesting it is part of a regulatory machinery assembled onto vesicles in preparation for exocytosis. Immunofluorescence, subcellular fractionation, live imaging of frog motor end plates The Journal of cell biology High 1655810
1990 Rab3A is associated with chromaffin granule membranes in adrenal medulla chromaffin cells; it is partially cytosolic and partially membrane-bound, and membrane association requires hydrophobic modification (likely fatty acid acylation or lipid anchor), consistent with a role in regulated secretion. Subcellular fractionation, immunoadsorption with anti-dopamine beta-hydroxylase antibody, detergent/salt extraction Proceedings of the National Academy of Sciences of the United States of America High 2165599
1991 Rab3A membrane attachment to synaptic vesicles is mediated by posttranslational polyisoprenylation (geranylgeranylation) of its C-terminal Cys-X-Cys sequence; this modification is required for membrane binding and is distinct from intracellular targeting to synaptic vesicles. Compactin inhibition (mevalonate-dependent), mutagenesis of C-terminal cysteines, biochemical fractionation Neuron High 1648935
1992 Rab3A effector domain peptides stimulate exocytotic fusion (degranulation) in mast cells in a Mg2+- and ATP-dependent, sequence-specific manner, suggesting activated Rab3A causes exocytotic fusion via an effector protein at the target membrane. Patch-clamp capacitance measurements, intracellular perfusion of synthetic peptides in mast cells Nature High 1331813
1992 A target protein for GTP-bound Rab3A (smg p25A) of ~85-86 kDa exists in bovine brain membranes; cross-linking is GTP-dependent and specific to Rab3A over other small GTPases, identifying the first putative Rab3A effector (later characterized as rabphilin-3A). Chemical cross-linking (disuccinimidyl suberate), SDS-PAGE, protein purification from brain membranes The Journal of biological chemistry High 1597436
1992 Rab3A effector domain peptide (rab3AL) stimulates amylase release from permeabilized pancreatic acini in an ATP-dependent manner, potentiates GTPγS-induced secretion, and lowers the Ca2+ threshold for secretion, demonstrating a role for Rab3-like proteins in a distal step of regulated secretion. Streptolysin-O permeabilized pancreatic acinar cells, amylase release assay Proceedings of the National Academy of Sciences of the United States of America High 1371881
1992 The geranylgeranyl moiety, but not the carboxymethyl moiety, of Rab3A is essential for interactions with membranes and with the GDP dissociation inhibitor (GDI); unmodified recombinant Rab3A lacks both activities. In vitro geranylgeranylation assay with purified geranylgeranyltransferase, membrane-binding assay, GDI sensitivity assay The Journal of biological chemistry High 1315770
1993 Rabphilin-3A is a GTP-dependent effector of Rab3A: it forms a complex specifically with GTPγS-bound (not GDP-bound) Rab3A, contains two C2 domains homologous to synaptotagmin, and its N-terminal domain binds Rab3A while its C-terminal C2 domains bind Ca2+/phospholipid. cDNA cloning, pulldown/complex formation assay, domain deletion analysis with recombinant fragments Molecular and cellular biology High 8384302
1993 The N-terminal domain of rabphilin-3A binds GTP-Rab3A and inhibits Rab3A-GAP-stimulated GTPase activity, thereby prolonging the GTP-bound active state of Rab3A; the C-terminal C2 domain does not bind Rab3A but mediates Ca2+/phospholipid binding. Recombinant fragment domain analysis, GTPase activity assay, GAP inhibition assay The Journal of biological chemistry High 8226731 8262955
1991 Rab3A has a detergent-soluble, brain-membrane-associated GAP activity that accelerates its GTPase; the GAP activity is thermolabile, trypsin-sensitive, and behaves as an integral membrane protein; a cytosolic GAP activity is also present. GTPase activity assay, membrane/cytosol fractionation, gel filtration chromatography The Journal of biological chemistry High 1847129
1993 The effector domain (residues 51-59) of Rab3A is required for interaction with Rab3A-GRF (guanine nucleotide releasing factor) and for cross-linking to the putative target protein p85; mutations in this domain abolish GRF sensitivity and most interactions, while the first G-domain has modest effects. Site-directed mutagenesis, GRF activity assay, GAP activity assay, cross-linking The Journal of biological chemistry High 8226229 8387493
1993 Rab3A GTPase cycle is regulated by GDP dissociation inhibitor (GDI), guanine nucleotide releasing factor (GRF), and GAP; in PC12 cells, cytosolic Rab3A is predominantly GDP-bound, while membrane-associated Rab3A is ~50% GTP-bound; GDI acts only on GDP-Rab3A and antagonizes GRF but not GAP. Nucleotide binding assay, GDI/GRF/GAP activity assays in PC12 cell fractions The Journal of biological chemistry High 8226729
1994 Rab geranylgeranyltransferase, together with Rab escort protein, catalyzes geranylgeranylation of both adjacent C-terminal cysteines of Rab3A (Cys-Ala-Cys-Cys motif), as established by mass spectrometric analysis of in vitro prenylated proteins. In vitro prenylation assay with purified enzyme, tryptic peptide HPLC, electrospray mass spectrometry Proceedings of the National Academy of Sciences of the United States of America High 7991565
1994 Rab3A plays a role in the recruitment of synaptic vesicles for exocytosis during repetitive stimulation but is not essential for basal exocytosis; rab3A-null mice show increased synaptic depression after short trains of stimuli and 70% reduction in rabphilin levels at synapses. Homologous recombination knockout, electrophysiological recordings in hippocampal CA1 cells, protein quantitation by immunoblot Nature High 7911226
1994 Synaptic targeting of rabphilin-3A depends on Rab3A (and Rab3C); in rab3A-deficient mice, rabphilin-3A is decreased in synapses of neurons primarily expressing rab3A and accumulates in perikarya; rabphilin-3A binds Rab3C in vitro, explaining rescue in neurons expressing Rab3C. Rab3A knockout mice analysis, immunocytochemistry, in vitro binding assay Neuron High 7946335
1994 Rab3A overexpression or expression of a constitutively GTP-bound mutant (Q81L) inhibits Ca2+-dependent exocytosis in chromaffin cells; this inhibition acts as a 'prefusion block', suggesting Rab3A may be an inhibitor of secretion that is overcome by elevated Ca2+. Transient transfection of chromaffin cells, human growth hormone reporter assay for exocytosis, Ca2+-stimulated secretion from permeabilized cells The Journal of biological chemistry High 8144603
1994 GTP cleavage by synaptic vesicle-bound Rab3A occurs during exocytosis: membrane-associated Rab3A is predominantly GTP-bound at rest, while cytosolic Rab3A is GDP-bound; alpha-latrotoxin-induced exocytosis causes a significant increase in GDP/GTP ratio of Rab3A. Nucleotide binding analysis of synaptosome fractions, alpha-latrotoxin stimulation, GDP/GTP ratio measurement The Journal of biological chemistry High 7929154
1994 Rab3A effector domain peptides specifically stimulate insulin exocytosis in electroporated beta-cells and interact with a cytosolic protein doublet (REEP-1 and REEP-2) via photocrosslinking; these proteins are membrane-associated under basal conditions and released to cytosol upon exocytosis stimulation. Electroporation of beta-cells, insulin release assay, 125I-radiolabeled photoactivatable crosslinking peptide The Journal of biological chemistry Medium 7961732
1994 Rabphilin-3A is phosphorylated by cAMP-dependent protein kinase (PKA) at its N-terminal region (approximately 0.8 mol phosphate per mol protein); Rab3A itself is not a PKA substrate. In vitro phosphorylation assay with purified PKA and recombinant proteins Biochemical and biophysical research communications High 7945346
1995 Rabphilin-3A overexpression enhances regulated secretion in chromaffin cells (~30%), while antisense inhibition reduces it; C2 domain deletion mutants strongly inhibit exocytosis despite retaining the Rab3A-binding domain, indicating the C2 domains are required for rabphilin-3A's positive regulatory function downstream of Rab3A interaction. cDNA transfection in chromaffin cells, growth hormone reporter assay, permeabilized cell secretion assay The Journal of biological chemistry High 7622481
1995 The Cys-rich zinc-finger domain of rabphilin-3A binds two Zn2+ ions and is necessary but not sufficient for Rab3A binding; a minimal Rab3A-binding domain spans residues 45-170; Rab3A targeting to vesicles is independent of its interaction with rabphilin-3A (Rab3A T54A mislocalizes from rabphilin but not from vesicles). Domain deletion/mutagenesis, GFP-fusion localization in PC12 cells, metal binding assay Molecular and cellular biology High 8756657
1995 Rabin3 (a novel 50-kDa brain protein) interacts specifically with Rab3A and Rab3D via the Rab3A effector domain; multiple effector domain mutations abolish the interaction; Rabin3 has sequence similarity to yeast Sec2p (a GEF for Sec4p, the Rab3A yeast ortholog), suggesting a conserved GEF-like role. Yeast two-hybrid screen, effector domain mutagenesis, GST pulldown Molecular and cellular biology Medium 7532276
1997 Ca2+/calmodulin causes Rab3A to dissociate from synaptic membranes in vitro by forming a 1:1 complex with Rab3A that requires both the lipidated C terminus and bound guanine nucleotide; this differs from GDI in being Ca2+-dependent and less stringently requiring GDP. In vitro membrane dissociation assay, complex formation assay, synthetic peptide competition The Journal of biological chemistry High 9252412
1997 Rab3A acts at a late step in synaptic vesicle fusion (after docking): rab3A-null mice have a normal readily releasable pool size but altered Ca2+-triggered fusion, with more exocytic events occurring within a brief time window after nerve impulse arrival. Electrophysiological analysis of rab3A-deficient mice, analysis of readily releasable pool, quantal analysis Nature High 9194562
1997 Rab3A is essential for mossy fiber LTP in the hippocampus; rab3A-null mice show abolishment of LTP at hippocampal mossy fiber synapses while short-term plasticities remain normal, placing Rab3A as a required presynaptic component of this NMDA-independent form of LTP. Rab3A knockout mice, hippocampal slice electrophysiology, LTP induction protocols Nature High 9252190
1998 In mossy fiber synapses, cAMP enhances glutamate release by multiple mechanisms including direct activation of the secretory apparatus (Ca2+ sensitivity), and only this last mechanism requires Rab3A; forskolin still enhances KCl- and sucrose-induced release in rab3A-deficient synaptosomes but fails to enhance ionomycin-induced release. CA3 synaptosome preparations from rab3A-null mice, glutamate release assay with multiple stimuli Neuron High 9856469
1999 Crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A (2.6 Å resolution) reveals two interfaces: one involving Rab3A switch I and switch II regions (nucleotide-state sensitive), and a second involving a unique deep pocket (RabCDR) that interacts with the SGAWFF element of rabphilin-3A and determines effector specificity. X-ray crystallography at 2.6 Å Cell High 10025402
1999 Rab3A is associated with the acrosomal membrane in rat sperm; synthetic Rab3 effector domain peptide inhibits ionophore-triggered acrosomal exocytosis in a concentration-dependent manner, suggesting Rab3A acts as an inhibitory regulator of the acrosome reaction. Immunogold EM, sucrose gradient fractionation, acrosome reaction assay with effector peptide Developmental biology Medium 10373312
2000 GTP-bound Rab3A triggers acrosomal exocytosis in permeabilized human spermatozoa; GDP-bound Rab3A and Rab11-GTP are inactive; recombinant GDI inhibits GTPγS-stimulated exocytosis, indicating Rab3A (or a Rab3 isoform) is a required positive regulator of acrosomal exocytosis. Streptolysin-O permeabilized sperm, recombinant Rab3A loaded with GTP/GDP, acrosome reaction assay Biology of reproduction High 10727281
2000 Calcium-dependent acrosomal exocytosis requires both active Rab3A (GTP-bound) and NSF; Rab3A activation protects NSF from NEM inhibition and prevents exchange of endogenous NSF with dominant-negative NSF mutants; Rab3A and NSF act in a coordinated cascade for acrosome fusion. Permeabilized sperm exocytosis assay, NEM inhibition, dominant-negative NSF protein microinjection Proceedings of the National Academy of Sciences of the United States of America High 10954749
2001 GRAB is a physiological GEF for Rab3A: it directly catalyzes GDP/GTP exchange on Rab3A, interacts with InsP6K1, and regulates depolarization-induced dopamine and growth hormone release from neuroendocrine cells. Protein cloning, GEF activity assay, dopamine release assay in PC12 cells, chromaffin cell secretion assay Neuron High 11516400
2001 Rab3A is required for activity-dependent recruitment of synaptic vesicles to and docking at the active zone: rab3A deletion completely abolishes depolarization-induced vesicle accumulation near active zones without affecting resting vesicle number or single-stimulus secretion; replenishment of docked vesicles after exhaustive stimulation is also impaired. Electron microscopy of nerve terminals from rab3A-null mice, vesicle distribution quantitation, secretion assay Molecular biology of the cell High 11598194
2003 Rim1 interacts with Rab3A/B/C/D and other Rabs; Rim2 interacts with Rab3A/B/C/D and Rab8A; an acidic cluster (Glu-50, Glu-51, Glu-52) in the first alpha-helical region of Rim2's Rab-binding domain is a critical determinant of Rab3A recognition, as shown by mutagenesis and chimeric analysis. Cotransfection assay with 42 Rab proteins, site-directed mutagenesis, chimeric protein analysis The Journal of biological chemistry High 12578829
2003 Rabconnectin-3 consists of alpha and beta subunits; the beta subunit directly binds Rab3 GEP (the GEF for Rab3A), while the alpha subunit indirectly associates with Rab3 GAP, forming a complex that coordinates Rab3A GTPase cycle regulation at synaptic vesicles. Co-immunoprecipitation from synaptic vesicle fractions, cDNA cloning, direct binding assay Genes to cells Medium 12786944
2003 Rab3A null mice develop fasting hyperglycemia and ablated first-phase insulin release in vivo; isolated Rab3A-null islets show ~60-70% reduction in secretagogue-induced insulin release with normal glucose oxidation and Ca2+ flux, placing Rab3A function downstream of Ca2+ signaling at the level of secretory granule transport/exocytosis. Rab3A knockout mice, glucose tolerance test, isolated islet insulin release, glucose oxidation and Ca2+ flux assays The Journal of biological chemistry High 12510060
2004 Synapsin I is a Rab3A effector on synaptic vesicles: it stimulates GTP binding and GTPase activity of Rab3A; conversely, Rab3A inhibits synapsin I binding to F-actin and actin bundling; synapsin I prevents RabGDI-induced Rab3A dissociation from vesicles; Rab3A levels on vesicles are reduced in synapsin KO mice. In vitro GTPase/GTP binding assay, F-actin binding/bundling assay, RabGDI dissociation assay, synapsin KO mice The Journal of biological chemistry High 15265868
2005 Zn7-metallothionein-3 binds reversibly to Rab3A in its GDP-bound form (Kd = 2.6 µM) but not to GTP-Rab3A; the interaction site maps to the effector binding region; GDP exchange kinetics are unaffected by the interaction, indicating Zn7MT-3 is not a GEF but may regulate Rab3A via its effector domain. Affinity precipitation, surface plasmon resonance, Rab3A mutagenesis, GDP exchange kinetics assay Biochemistry High 15736926
2006 Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicles to the plasma membrane in PC12 cells: siRNA silencing of either reduces docked vesicle number without altering exocytotic kinetics; simultaneous silencing causes a significantly greater decrease in docking. siRNA knockdown, TIRF microscopy single-cell analysis in PC12 cells Journal of cell science High 16684812
2006 Active zone recruitment of Munc13-1 and ubMunc13-2 requires binding to RIM1α; a single point mutation (I121N) in Munc13s abolishes RIM1α (Rab3A-interacting molecule) binding and prevents synaptic recruitment of Munc13s; Munc13-1 levels and active zone enrichment are reduced in RIM1α-deficient brain. Point mutagenesis, co-IP, RIM1α KO mice immunostaining and Western blot The Journal of biological chemistry High 16704978
2007 FRAP analysis shows EGFP-Rab3A exchanges rapidly between granules and cytosol (faster recovery than Rab27A or granule cargo ppANF), consistent with a GTP hydrolysis-dependent cycle; newly synthesized secretory granules preferentially recruit Rab3A and Rab27A, suggesting these Rabs mark young granules for preferential exocytosis. FRAP in PC12 cells, post-transfection time-course of granule association, live cell imaging during stimulation Journal of cell science High 17311845
2007 Rab3A cycling between GTP and GDP forms (not either locked state alone) is required for its docking function; both GTP- and GDP-locked Rab3A mutants fail to promote vesicle docking; furthermore, the docking function of Rab3A requires Munc18-1, as wild-type Rab3A cannot promote docking in munc18-1 null chromaffin cells. Expression of Rab3A mutants in wild-type and munc18-1 null chromaffin cells, electron microscopy vesicle distribution PloS one High 17637832
2007 Rab3A deletion reduces vesicle docking (26% reduction) and quantal release (27% reduction in quantal content, 28% reduction in mini frequency) at the mouse diaphragm neuromuscular junction; Ca2+ sensitivity (not cooperativity) of release is affected. rab3A-null mice, electron microscopy, focal electrophysiological recordings, Ca2+ concentration-response Neuroscience High 17640821
2008 FLJ13130 (TBC domain protein) is a novel Rab3A-GAP: its expression promotes GTPase activity of Rab3A in vitro and reduces GTP-Rab3A levels in living cells; a catalytically inactive R134K mutant is ineffective; FLJ13130 also acts on Rab22A, Rab27A, and Rab35 but not Rab2A or Rab6A. Cell-based screen for Rab3A exclusion from dense-core vesicles, in vitro GTPase activity assay, catalytic mutant control Genes to cells High 19077034
2009 APP anterograde transport vesicles contain kinesin-1C, Rab3A, and a specific subset of presynaptic proteins; assembly of kinesin-1C and APP in this vesicle requires Rab3A GTPase activity, as shown by immunoisolation and time-lapse analysis. Time-lapse microscopy, immunoisolation of transport vesicles, GTPase-deficient Rab3A mutant analysis The Journal of neuroscience High 19923287
2009 Epac activates Rab3A (promotes GDP→GTP exchange) in human sperm downstream of cAMP/Epac/Rap1/PLC signaling during acrosomal exocytosis; recombinant Epac does not directly exchange GDP from Rab3A in vitro, indicating an indirect GEF activation pathway. GTP/GDP loading assay in sperm, recombinant Epac in vitro exchange assay, pharmacological inhibitors The Journal of biological chemistry High 19546222
2009 SNAP-29 interacts with Rab3A in a GTP-dependent manner (yeast two-hybrid and coimmunoprecipitation); coexpression of SNAP-29 and Rab3A redistributes cytoplasmic SNAP-29 and enhances surface-directed trafficking of myelin proteolipid protein, placing Rab3A upstream of SNAP-29-mediated membrane fusion. Yeast two-hybrid, co-immunoprecipitation, HEK293 trafficking assay Journal of neuroscience research Medium 19170188
2010 Mass spectrometry and quantitative immunoblotting identify Rab3A (along with Rab3b, Rab3c, Rab27b) as exocytotic Rab machinery on synaptic vesicles; Rab3A readily dissociates from SVs during Ca2+-triggered exocytosis and is susceptible to GDI-mediated membrane extraction, whereas Rab27b persists on vesicle membranes after stimulation. High-resolution mass spectrometry, iTRAQ chemical labeling, quantitative immunoblotting, fluorescence microscopy, stimulation and GDI extraction assay The Journal of neuroscience High 20926670
2011 Myo5a (myosin-Va) tail directly interacts with GTP-bound Rab3A on synaptic vesicles: the interaction requires GTP (not GDP or nucleotide-free Rab3A), is demonstrated by sedimentation velocity analytical ultracentrifugation, GST pulldown from synaptosomes, and in vitro motility assays requiring Rab GTPase activity. Analytical ultracentrifugation (sedimentation velocity), GST pulldown from synaptosomes, in vitro motility assay in squid axoplasm The Journal of biological chemistry High 21349835
2011 Rab3A cycle (via RIM interaction) is coupled with Munc13-1 activation for vesicle priming; Munc18-1 promotes Rab3A dissociation from vesicles and acts downstream of the Munc13-1/RIM/Rab3A complex to enable vesicle priming and fusion. Rab3A overexpression/knockdown, Munc13-1/Munc18-1 co-expression, secretion assays in neuroendocrine cells Traffic Medium 21689256
2012 RIM, Munc13, and Rab3A are all present in human sperm acrosomal region and participate in a pre-fusion docking step during acrosomal exocytosis; sequestering RIM or Rab3A (by antibody or recombinant protein) impairs docking of the acrosomal membrane to the plasma membrane. Immunostaining, functional inhibition with antibodies/recombinant proteins, transmission electron microscopy of docking Experimental cell research Medium 22248876
2012 Rab3A delivers synaptic vesicles to Ca2+-dependent release sites at ribbon synapses in photoreceptors; GTPase-deficient Rab3A blocks synaptic release in an activity-dependent, frequency-dependent manner by competing with vesicles for resupply to release sites; ribbon binding and dissociation are governed by the GTP hydrolysis cycle. Fluorescent Rab3A delivery via patch pipette, GTPase-deficient mutant expression, paired pre- and postsynaptic recordings The Journal of neuroscience High 22593061
2013 α-Synuclein interacts with membrane-associated GTP-bound Rab3A but not cytosolic GDP-Rab3A; GTPase-deficient Rab3A mutant, dominant-negative GDI (unable to recycle Rab3A from membranes), and Hsp90 inhibitors all increase membrane-bound α-synuclein, indicating that the GDI·Hsp90 complex controlling Rab3A recycling also regulates α-synuclein membrane association. Density gradient sedimentation, co-immunoprecipitation, GTPase-deficient mutant, dominant-negative GDI, Hsp90 inhibitors The Journal of biological chemistry High 23344955
2013 β-Adrenergic receptor activation via cAMP/Epac increases the Rab3A–RIM1α association and redistributes synaptic vesicles closer to the presynaptic membrane to potentiate glutamate release, independently of PKA. Co-immunoprecipitation, synaptic vesicle redistribution by EM, glutamate release assay in cerebrocortical synaptosomes The Journal of biological chemistry Medium 24036110
2015 ARF6 activation during acrosomal exocytosis increases GDP→GTP exchange on Rab3A (a prerequisite for exocytosis), acting via PLC/PIP2 signaling; ARF6 thus functions upstream of Rab3A in the acrosomal exocytosis cascade. GTP/GDP loading assay on Rab3A in sperm, pulldown assays, permeabilized sperm exocytosis, ARF6 inhibition The Journal of biological chemistry Medium 25713146
2016 Rab3A partially localizes to peripheral lysosomes and is required for lysosome positioning and plasma membrane repair (PMR); Rab3A forms a complex with its effectors Slp4-a (synaptotagmin-like protein 4a) and non-muscle myosin heavy chain IIA (NMHC IIA) to position lysosomes at the cell periphery for exocytosis and PMR. siRNA screen of Rab family, Rab3A silencing, lysosome localization by imaging, PMR assay (streptolysin-O), co-immunoprecipitation identifying NMHC IIA as effector The Journal of cell biology High 27325790
2016 Mutant huntingtin (mHtt) associates with Rab3A and prevents GTP-Rab3A from binding Rab3-GAP1, disrupting GTP→GDP conversion; this impairs BDNF vesicle docking on astrocyte plasma membranes; Rab3A overexpression rescues BDNF vesicle docking and secretion in HD astrocytes. Co-immunoprecipitation of mHtt and Rab3A, BDNF vesicle docking assay, Rab3A overexpression rescue in HD knock-in astrocytes The Journal of neuroscience High 27559163
2018 O-GlcNAcylation of Rab3A attenuates its GTP-binding activity and suppresses its effects on mitochondrial oxidative phosphorylation and hepatocellular carcinoma cell metastasis; O-GlcNAcylation and Rab3A have opposing functional effects on these processes. O-GlcNAc modification identification, GTP-binding assay, in vitro and in vivo metastasis assays, mitochondrial OXPHOS measurement Cell death & disease Medium 30237463
2021 Rab11 regulates Ca2+-induced lysosome exocytosis via a cascade involving Rab3A: Rab11-positive vesicles transiently interact with peripheral lysosomes; Rab11 binds GRAB (Rab3A GEF) and Rab3A, suggesting a Rab11→GRAB→Rab3A signaling cascade for lysosome exocytosis. siRNA silencing of Rab11a/b, live imaging of Rab11–lysosome interactions, co-immunoprecipitation of Rab11 with GRAB and Rab3A, lysosome exocytosis assay Journal of cell science Medium 34100549

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1994 The role of Rab3A in neurotransmitter release. Nature 428 7911226
1993 Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin. Molecular and cellular biology 378 8384302
1997 The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion. Nature 359 9194562
1999 Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell 299 10025402
1997 Rab3A is essential for mossy fibre long-term potentiation in the hippocampus. Nature 297 9252190
1991 Association of Rab3A with synaptic vesicles at late stages of the secretory pathway. The Journal of cell biology 227 1655810
1994 Evidence for the involvement of Rab3A in Ca(2+)-dependent exocytosis from adrenal chromaffin cells. The Journal of biological chemistry 209 8144603
1990 Association of the GTP-binding protein Rab3A with bovine adrenal chromaffin granules. Proceedings of the National Academy of Sciences of the United States of America 209 2165599
1994 Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron 181 7946335
1992 Exocytotic fusion is activated by Rab3a peptides. Nature 167 1331813
2003 Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. The Journal of biological chemistry 159 12578829
2006 Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. Journal of cell science 158 16684812
1993 Two functionally different domains of rabphilin-3A, Rab3A p25/smg p25A-binding and phospholipid- and Ca(2+)-binding domains. The Journal of biological chemistry 136 8262955
1994 Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A. Proceedings of the National Academy of Sciences of the United States of America 134 7991565
1992 A synthetic peptide of the rab3a effector domain stimulates amylase release from permeabilized pancreatic acini. Proceedings of the National Academy of Sciences of the United States of America 133 1371881
1995 Evidence that the Rab3a-binding protein, rabphilin3a, enhances regulated secretion. Studies in adrenal chromaffin cells. The Journal of biological chemistry 128 7622481
2003 Insulin secretory deficiency and glucose intolerance in Rab3A null mice. The Journal of biological chemistry 109 12510060
1992 A possible target protein for smg-25A/rab3A small GTP-binding protein. The Journal of biological chemistry 106 1597436
1998 Mechanism of action of rab3A in mossy fiber LTP. Neuron 105 9856469
2009 APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle. The Journal of neuroscience : the official journal of the Society for Neuroscience 102 19923287
1996 Rab3A small GTP-binding protein in Ca(2+)-dependent exocytosis. Genes to cells : devoted to molecular & cellular mechanisms 100 9078389
1991 rab3A attachment to the synaptic vesicle membrane mediated by a conserved polyisoprenylated carboxy-terminal sequence. Neuron 97 1648935
2016 A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair. The Journal of cell biology 90 27325790
1999 Subcellular distribution and function of Rab3A, B, C, and D isoforms in insulin-secreting cells. Molecular endocrinology (Baltimore, Md.) 90 9973251
2001 Rab3a is involved in transport of synaptic vesicles to the active zone in mouse brain nerve terminals. Molecular biology of the cell 89 11598194
1993 Mutants of Rab3A analogous to oncogenic Ras mutants. Sensitivity to Rab3A-GTPase activating protein and Rab3A-guanine nucleotide releasing factor. The Journal of biological chemistry 87 8387493
2013 α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity. The Journal of biological chemistry 86 23344955
2010 Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. The Journal of neuroscience : the official journal of the Society for Neuroscience 86 20926670
2016 Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP. The Journal of neuroscience : the official journal of the Society for Neuroscience 85 27559163
2001 GRAB: a physiologic guanine nucleotide exchange factor for Rab3A, which interacts with inositol hexakisphosphate kinase. Neuron 85 11516400
2006 Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2. The Journal of biological chemistry 84 16704978
2009 Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis. The Journal of biological chemistry 83 19546222
2000 Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor. Proceedings of the National Academy of Sciences of the United States of America 82 10954749
2000 Rab3A triggers the acrosome reaction in permeabilized human spermatozoa. Biology of reproduction 80 10727281
1992 The geranylgeranyl moiety but not the methyl moiety of the smg-25A/rab3A protein is essential for the interactions with membrane and its inhibitory GDP/GTP exchange protein. The Journal of biological chemistry 80 1315770
1991 Regulation of the GTPase activity of the ras-like protein p25rab3A. Evidence for a rab3A-specific GAP. The Journal of biological chemistry 77 1847129
1992 Amino acid residues in the Ras-like GTPase Rab3A that specify sensitivity to factors that regulate the GTP/GDP cycling of Rab3A. The Journal of biological chemistry 76 1331063
1999 Identification of Rab3A GTPase as an acrosome-associated small GTP-binding protein in rat sperm. Developmental biology 74 10373312
1996 Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. The Journal of biological chemistry 73 8636125
1995 Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A. Molecular and cellular biology 71 7532276
1997 Ca2+/calmodulin causes Rab3A to dissociate from synaptic membranes. The Journal of biological chemistry 70 9252412
2002 Mutations in Rab3a alter circadian period and homeostatic response to sleep loss in the mouse. Nature genetics 68 12244319
1995 Nonneuronal expression of Rab3A: induction during adipogenesis and association with different intracellular membranes than Rab3D. Proceedings of the National Academy of Sciences of the United States of America 63 7753798
1993 Rab3A GTPase-activating protein-inhibiting activity of Rabphilin-3A, a putative Rab3A target protein. The Journal of biological chemistry 63 8226731
1994 Rabphilin-3A is associated with synaptic vesicles through a vesicle protein in a manner independent of Rab3A. The Journal of biological chemistry 61 7806490
2001 Rab3A is required for brain-derived neurotrophic factor-induced synaptic plasticity: transcriptional analysis at the population and single-cell levels. The Journal of neuroscience : the official journal of the Society for Neuroscience 60 11517266
1995 The GTPase Rab3a is associated with large dense core vesicles in bovine chromaffin cells and rat PC12 cells. Journal of cell science 57 7615682
2007 Differential dynamics of Rab3A and Rab27A on secretory granules. Journal of cell science 56 17311845
2004 Synapsin is a novel Rab3 effector protein on small synaptic vesicles. II. Functional effects of the Rab3A-synapsin I interaction. The Journal of biological chemistry 56 15265868
1996 Role of the Rab3A-binding domain in targeting of rabphilin-3A to vesicle membranes of PC12 cells. Molecular and cellular biology 54 8756657
1994 GTP cleavage by the small GTP-binding protein Rab3A is associated with exocytosis of synaptic vesicles induced by alpha-latrotoxin. The Journal of biological chemistry 52 7929154
2003 A novel rabconnectin-3-binding protein that directly binds a GDP/GTP exchange protein for Rab3A small G protein implicated in Ca(2+)-dependent exocytosis of neurotransmitter. Genes to cells : devoted to molecular & cellular mechanisms 51 12786944
2005 Zn7metallothionein-3 and the synaptic vesicle cycle: interaction of metallothionein-3 with the small GTPase Rab3A. Biochemistry 47 15736926
2003 Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. The American journal of pathology 47 12937130
2008 Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes to cells : devoted to molecular & cellular mechanisms 46 19077034
2004 Mice deficient for the synaptic vesicle protein Rab3a show impaired spatial reversal learning and increased explorative activity but none of the behavioral changes shown by mice deficient for the Rab3a regulator Gdi1. The European journal of neuroscience 46 15078563
2009 The SNARE protein SNAP-29 interacts with the GTPase Rab3A: Implications for membrane trafficking in myelinating glia. Journal of neuroscience research 45 19170188
2008 Loss of RAB-3/A in Caenorhabditis elegans and the mouse affects behavioral response to ethanol. Genes, brain, and behavior 44 18397381
2011 Glucagon-like peptide-1 enhances glucokinase activity in pancreatic β-cells through the association of Epac2 with Rim2 and Rab3A. Endocrinology 43 22147008
2000 Rab3A and Rab3D control the total granule number and the fraction of granules docked at the plasma membrane in PC12 cells. Traffic (Copenhagen, Denmark) 42 11208087
2016 Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair. Small GTPases 41 27687479
2001 Rab3a and SNARE proteins: potential regulators of melanosome movement. The Journal of investigative dermatology 41 11180007
2013 β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminals. The Journal of biological chemistry 40 24036110
2007 Rab3a deletion reduces vesicle docking and transmitter release at the mouse diaphragm synapse. Neuroscience 38 17640821
1999 The monomeric GTP binding protein, rab3a, is associated with the acrosome in mouse sperm. Molecular reproduction and development 38 10398417
1994 Rab3A effector domain peptides induce insulin exocytosis via a specific interaction with a cytosolic protein doublet. The Journal of biological chemistry 38 7961732
2021 Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB. Journal of cell science 37 34100549
2012 RIM, Munc13, and Rab3A interplay in acrosomal exocytosis. Experimental cell research 36 22248876
2005 Alpha-synuclein binding to rab3a in multiple system atrophy. Neuroscience letters 36 15854772
1993 The Rab3A GTPase interacts with multiple factors through the same effector domain. Mutational analysis of cross-linking of Rab3A to a putative target protein. The Journal of biological chemistry 36 8226995
2007 The role of Rab3a in secretory vesicle docking requires association/dissociation of guanidine phosphates and Munc18-1. PloS one 34 17637832
1993 Structure of the murine rab3A gene: correlation of genomic organization with antibody epitopes. The Biochemical journal 33 7687127
2014 Rab3a promotes brain tumor initiation and progression. Molecular biology reports 32 24965146
2000 Evidence of Rab3A expression, regulation of vesicle trafficking, and cellular secretion in response to heregulin in mammary epithelial cells. Molecular and cellular biology 31 11074007
1997 Rat basophilic leukaemia (RBL) cells overexpressing Rab3a have a reversible block in antigen-stimulated exocytosis. The Biochemical journal 31 9163319
2018 O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma. Cell death & disease 30 30237463
2015 ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation. The Journal of biological chemistry 30 25713146
2001 Rab3a binding and secretion-enhancing domains in Rim1 are separate and unique. Studies in adrenal chromaffin cells. The Journal of biological chemistry 30 11278839
1995 Rab3a, a small GTP-binding protein, undergoes fast anterograde transport but not retrograde transport in neurons. European journal of cell biology 30 8521869
2011 Involvement of Rab3A in vesicle priming during exocytosis: interaction with Munc13-1 and Munc18-1. Traffic (Copenhagen, Denmark) 29 21689256
1993 Regulation of the GTPase cycle of the neuronally expressed Ras-like GTP-binding protein Rab3A. The Journal of biological chemistry 29 8226729
2021 Small Rab GTPases in Intracellular Vesicle Trafficking: The Case of Rab3A/Raphillin-3A Complex in the Kidney. International journal of molecular sciences 28 34299299
2011 Myosin5a tail associates directly with Rab3A-containing compartments in neurons. The Journal of biological chemistry 28 21349835
2007 Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse. Genes, brain, and behavior 28 16734774
2003 Rab3A negatively regulates activity-dependent modulation of exocytosis in bovine adrenal chromaffin cells. The Journal of physiology 28 14694148
1995 Double in situ hybridization reveals overlapping neuronal populations expressing the low molecular weight GTPases Rab3a and Rab3b in Rat brain. The European journal of neuroscience 28 7620619
1994 Phosphorylation of Rabphilin-3A, a putative target protein for Rab3A, by cyclic AMP-dependent protein kinase. Biochemical and biophysical research communications 28 7945346
2002 Rab3A and calmodulin regulate acrosomal exocytosis by mechanisms that do not require a direct interaction. FEBS letters 27 12163174
1998 Regulation of the Ca2+ sensitivity of exocytosis by Rab3a. Journal of neurochemistry 27 9721737
1992 Localization of smg p25A/rab3A p25, a small GTP-binding protein, at the active zone of the rat neuromuscular junction. Biochemical and biophysical research communications 27 1324664
2006 Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs. American journal of physiology. Cell physiology 26 16822953
2001 Reduced expression of amyloid precursor protein, presenilin-1 and rab3a in cortical brain regions in Alzheimer's disease. Dementia and geriatric cognitive disorders 26 11351135
2000 Small Gtpase rab3A is associated with melanosomes in melanoma cells. Pigment cell research 26 11041209
2016 Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation. Histochemistry and cell biology 25 26791531
1997 Rab3A immunolocalization in the mammalian vestibular end-organs during development and comparison with synaptophysin expression. Brain research. Developmental brain research 24 9088571
1992 Localization of the ras-like rab3A protein in the adult rat brain. Brain research 24 1330210
2012 Rab3A mediates vesicle delivery at photoreceptor ribbon synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience 22 22593061
2009 Rab3a interacting molecule (RIM) and the tethering of pre-synaptic transmitter release site-associated CaV2.2 calcium channels. Journal of neurochemistry 22 19878533
1994 Developmental changes in the localization of the synaptic vesicle protein rab3A in rat brain. Neuroscience 22 7830899
2000 Reduction of the synaptic protein rab3a in the thalamus and connecting brain regions in post-mortem schizophrenic brains. Journal of neural transmission (Vienna, Austria : 1996) 21 11041284