Affinage

RPH3A

Rabphilin-3A · UniProt Q9Y2J0

Length
694 aa
Mass
76.9 kDa
Annotated
2026-06-10
90 papers in source corpus 50 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Rabphilin-3A (RPH3A) is a multidomain synaptic effector that couples Rab GTPase signaling to the membrane fusion machinery, regulating both presynaptic vesicle exocytosis and postsynaptic receptor stabilization (PMID:8384302, PMID:16763567, PMID:26679993). Its N-terminal Zn2+-finger domain binds the GTP-bound forms of Rab3A and, with higher functional relevance for vesicle recruitment, Rab27A, which reversibly targets the protein to secretory and dense-core vesicles (PMID:8384302, PMID:8617225, PMID:12578829, PMID:14722103); the structural basis of this state-dependent recognition was defined by the Rab3A/GTP–rabphilin effector-domain crystal structure, in which an SGAWFF element engages a Rab pocket conferring effector specificity (PMID:10025402). Its tandem C2 domains bind Ca2+ and phospholipids including PIP2 — C2A via a Ca2+/IP3 target-activated (TAMA) mechanism — providing membrane-coincidence sensing (PMID:8262955, PMID:18434502, PMID:18945677). Presynaptically, the C2B domain directly binds the plasma-membrane SNARE SNAP-25 through a polybasic helix and bottom alpha-helix, promoting vesicle docking and accelerating SNARE complex assembly by inducing an alpha-helical conformational switch in the SNAP-25 SNARE motif (PMID:16203731, PMID:17156129, PMID:28634303, PMID:36173100); through this C2B–SNAP-25 interaction RPH3A modulates synaptic vesicle re-priming and acts as a negative regulator of dense-core vesicle exocytosis (PMID:16763567, PMID:39412498). Postsynaptically, RPH3A is enriched at dendritic spines where its N-terminal domain forms a ternary complex with GluN2A and PSD-95 and undergoes arginine-dependent liquid-liquid phase separation to cluster and stabilize synaptic GluN2A-containing NMDARs (PMID:26679993, PMID:36693856). RPH3A is a convergent phosphorylation target of PKA and CaMKII at Ser234/Ser274, modifications that reduce its membrane affinity, and is regulated by mono-ubiquitination and 14-3-3 binding (PMID:7891174, PMID:11466418, PMID:33542309, PMID:29441694). Disease-associated RPH3A missense variants impair 14-3-3 binding or perturb GluN2A retention and glutamate release, linking the protein to a presynaptic congenital myasthenic syndrome and to pre- and postsynaptic synaptic dysfunction (PMID:29441694, PMID:37403762, PMID:40082528). A distinct, non-neuronal role is established in neutrophils, where PKN1 phosphorylation of RPH3A enhances GTP-RAB21 binding to drive polarized vesicle trafficking and integrin activation (PMID:28636945, PMID:31924649).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1993 High

    Establishing what links Rab3A to its downstream effects: RPH3A was defined as the first GTP-state-dependent Rab3A effector, with separable Rab-binding and Ca2+/lipid-binding modules.

    Evidence In vitro pulldown of recombinant rabphilin with GTPgammaS- vs GDP-Rab3A, and domain-fragment binding assays with phospholipid and 45Ca2+

    PMID:8262955 PMID:8384302

    Open questions at the time
    • Did not establish the cellular consequence of the effector interaction
    • Domain boundaries refined only in later work
  2. 1993 High

    Addressing how RPH3A might influence Rab3A nucleotide state: it inhibits GAP-stimulated GTP hydrolysis and was reported to have exchange activity, positioning it to stabilize active Rab3A.

    Evidence In vitro GTPase and GDP/GTP exchange assays with purified Rab3A and rabphilin fragments

    PMID:7926025 PMID:8226731

    Open questions at the time
    • Physiological relevance of GAP inhibition/exchange activity in cells not established
    • Exchange activity reported by a single lab
  3. 1994 High

    Defining where RPH3A acts and how it gets there: it localizes to presynaptic synaptic vesicles, and its synaptic targeting depends on Rab3A/3C.

    Evidence Immunogold EM and subcellular fractionation of rat brain; immunofluorescence in rab3A knockout mice with in vitro rab3C binding

    PMID:7946335 PMID:8060298

    Open questions at the time
    • A Rab3A-independent vesicle association was also reported (idx 5), leaving recruitment mechanism only partly resolved
  4. 1994 High

    Identifying RPH3A as a kinase target: it is phosphorylated by PKA and CaMKII, marking it as a node integrating second-messenger signaling with release.

    Evidence In vitro phosphorylation assays with PKA and CaMKII and phosphopeptide site mapping

    PMID:7811264 PMID:7891174 PMID:7945346

    Open questions at the time
    • Functional consequence of individual sites not established at this stage
    • Early site assignments later refined to Ser234/Ser274
  5. 1996 High

    Clarifying the recruitment cycle and cytoskeletal coupling: a Zn2+-finger motif mediates GTP-Rab3A binding essential for vesicle recruitment, RPH3A dissociates upon Ca2+-dependent fusion, and its N-terminus binds alpha-actinin to modulate actin crosslinking.

    Evidence Exocytosis-coupled dissociation assays and Zn2+-finger mutagenesis; yeast two-hybrid plus actin crosslinking assays

    PMID:8617225 PMID:8943213

    Open questions at the time
    • In vivo significance of actin crosslinking modulation not established
    • Competition between Rab3A and alpha-actinin for the same region left functional logic unresolved
  6. 1996 High

    Testing functional requirement: RPH3A is required for Ca2+-regulated exocytosis, with C2B but not C2A fragments inhibiting release.

    Evidence Antisense knockdown and domain-fragment overexpression with growth hormone release readout in PC12 cells; presynaptic microinjection into squid giant synapse with electrophysiology and EM

    PMID:8605005 PMID:9450942

    Open questions at the time
    • Fragment overexpression is dominant-negative and does not pinpoint endogenous mechanism
    • Molecular target of C2B in release not yet identified
  7. 1999 High

    Providing structural rules for specificity and Ca2+-independent interactions: crystal and NMR structures defined the Rab3A effector interface and a Janus-faced C2B domain with a Ca2+-independent bottom surface.

    Evidence X-ray structure of Rab3A/GTP/Mg2+–rabphilin effector domain at 2.6 A; NMR solution structure of the C2B domain

    PMID:10025402 PMID:10559882

    Open questions at the time
    • Identity of the Ca2+-independent C2B partners not defined at this point
  8. 2001 High

    Uncovering Rab3-independent functions: secretory potentiation and endocytosis control can occur without high-affinity Rab3 binding, the latter via Rabaptin-5, and genetics revealed a Rab3-independent role in potentiating SNARE function.

    Evidence Rab3-binding-domain point mutagenesis with secretion and transferrin endocytosis assays in HIT-T15 cells; C. elegans genetic epistasis with SNARE hypomorphs

    PMID:10504306 PMID:11309205 PMID:11717359

    Open questions at the time
    • The unknown factor binding the Rab3-binding domain to drive secretion was not identified
    • Mechanistic link between rabphilin and SNAREs still inferred genetically
  9. 2003 High

    Broadening the effector repertoire and identifying the operative vesicle Rab: RPH3A binds multiple Rabs including Rab27, and Rab27A rather than Rab3A recruits it to dense-core vesicles.

    Evidence Co-transfection binding screen against 42 Rabs; mutagenesis and live-cell localization with neuropeptide Y secretion in PC12 cells across species

    PMID:12578829 PMID:14722103

    Open questions at the time
    • Relative in vivo contributions of Rab3 vs Rab27 across cell types not fully resolved
  10. 2005 High

    Identifying the molecular basis of docking: the C2B domain binds SNAP-25 and an alpha-actinin-dependent mechanism tethers RPH3A to the subplasmalemmal actin cytoskeleton, increasing docked vesicles.

    Evidence In vitro SNAP-25 binding with affinity measurement and TIRFM docking assays; F-actin reconstitution with purified components and granule co-sedimentation

    PMID:16043482 PMID:16203731

    Open questions at the time
    • Whether docking and re-priming use the same C2B-SNAP-25 contact left for later work
  11. 2006 High

    Mapping the docking motif and physiological role: a C2B polybasic sequence mediates SNAP-25 binding required for docking, and knockout/rescue shows RPH3A regulates SNARE-dependent vesicle re-priming through this interaction.

    Evidence Polybasic-sequence mutagenesis with binding and TIRFM exocytosis assays in PC12 cells; knockout neuron electrophysiology with WT and C2B-mutant viral rescue and synaptobrevin double knockout

    PMID:16763567 PMID:17156129

    Open questions at the time
    • Structural conformation enabling simultaneous SNAP-25 and membrane binding not yet resolved
  12. 2008 High

    Defining the lipid-sensing chemistry: both C2 domains bind PIP2, with C2A using a Ca2+/IP3 mutually-enhancing TAMA mechanism, and the structural conformational change enabling it was determined.

    Evidence NMR mapping of IP3 binding with affinity measurements; NMR solution structure of Ca2+-bound C2A with docking and CBL3 mutagenesis; C2A-C2B linker structural studies

    PMID:16790935 PMID:17166855 PMID:18434502 PMID:18945677 PMID:31924649

    Open questions at the time
    • In vivo PIP2 sensing during release not directly demonstrated in this set
  13. 2015 High

    Revealing a postsynaptic role: RPH3A forms a ternary complex with GluN2A and PSD-95 to stabilize synaptic NMDARs.

    Evidence Co-IP, GST pulldown, RNAi knockdown, patch clamp, confocal imaging, and in vivo interfering-peptide injection in organotypic slices

    PMID:26679993

    Open questions at the time
    • Mechanism by which complex stabilizes surface GluN2A not yet defined (resolved later by phase separation)
  14. 2017 High

    Defining how RPH3A engages the fusion machinery and is regulated in non-neuronal cells: C2B binds the same SNAP-25 surface as synaptotagmin-1 while simultaneously binding PIP2/Ca2+, and PKN1 phosphorylation directs RAB21 vesicle trafficking in neutrophils.

    Evidence Crystal structures of C2B-SNAP25 and C2B-PIP2 complexes with biochemical analysis; kinase assay, co-IP, neutrophil imaging, siRNA, and myeloid PKN1 knockout mice

    PMID:28634303 PMID:28636945

    Open questions at the time
    • How C2B coordinates with the complete assembled SNARE complex left for functional reconstitution
  15. 2022 High

    Establishing the catalytic action on SNARE assembly: RPH3A binds the SNAP-25 N-peptide via the C2B bottom helix, an intramolecular N-/C-terminal interplay enables it, and it induces an alpha-helical conformational switch accelerating SNARE complex assembly.

    Evidence In vitro SNARE assembly assays, NMR, binding studies, mutagenesis, and TIRF microscopy in PC12 cells

    PMID:36173100

    Open questions at the time
    • Quantitative contribution to fusion kinetics in native synapses not measured
  16. 2023 High

    Explaining how RPH3A stabilizes postsynaptic receptors: arginine-dependent liquid-liquid phase separation, promoted by the GluN2A/PSD-95 ternary complex, clusters and stabilizes synaptic GluN2A.

    Evidence In vitro phase separation assays, arginine mutagenesis, FRAP, super-resolution imaging, and electrophysiology in hippocampal neurons; GluN2A/NMDAR functional assays

    PMID:35626653 PMID:36693856

    Open questions at the time
    • Whether the same phase-separation property operates presynaptically not addressed
  17. 2024 High

    Resolving the direction of presynaptic regulation: RPH3A is a stationary presynaptic negative regulator of dense-core vesicle exocytosis acting through SNAP-25 binding, separable from its Rab3A-dependent synaptic enrichment.

    Evidence Single-vesicle live imaging in RPH3A knockout hippocampal neurons with WT and domain-specific mutant viral rescue and tetanus neurotoxin ablation

    PMID:39412498

    Open questions at the time
    • Reconciliation of negative DCV regulation with earlier positive secretion roles across cell types not fully resolved
  18. 2025 Medium

    Linking RPH3A dysfunction to disease: missense variants impair 14-3-3 binding, alter GluN2A retention and glutamate release, and produce pre- and postsynaptic dysfunction, connecting RPH3A to presynaptic congenital myasthenic syndrome and synaptic disorders.

    Evidence Variant 14-3-3 binding assays in cell lines; neuronal cultures with electrophysiology, calcium imaging, and GluN2A localization for multiple disease variants

    PMID:29441694 PMID:37403762 PMID:40082528

    Open questions at the time
    • Single-lab functional characterizations for most variants
    • Genotype-phenotype relationships across patients not established in this corpus

Open questions

Synthesis pass · forward-looking unresolved questions
  • How presynaptic SNARE regulation, postsynaptic NMDAR phase separation, and phosphorylation/ubiquitination control are coordinated within a single neuron, and how alpha-synuclein interaction integrates into neurodegenerative pathology, remain open.
  • No unified model integrating pre- and postsynaptic functions
  • Cellular regulation of the LLPS versus SNARE-assembly activities not defined
  • alpha-synuclein/RPH3A pathway mechanistically incomplete (idx 42)

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 4 GO:0008289 lipid binding 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005886 plasma membrane 4 GO:0031410 cytoplasmic vesicle 3 GO:0005829 cytosol 2 GO:0005856 cytoskeleton 1
Pathway
R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-112316 Neuronal System 3 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 2
Partners
ACTN (ALPHA-ACTININ)DLG4 (PSD-95)GLUN2A (GRIN2A)RAB21RAB27ARAB3ASNAP25UBE3A
Complex memberships
RPH3A/GluN2A/PSD-95 ternary complex

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 Rabphilin-3A (RPH3A) was identified as a novel protein that selectively interacts with the GTP-bound form of Rab3A but not the GDP-bound form, establishing it as a GTP-state-dependent effector of Rab3A. Biochemical pulldown/complex formation assay using GTPγS-bound vs. GDP-bound Rab3A with purified and recombinant rabphilin-3A; cDNA cloning and expression in COS7 cells Molecular and cellular biology High 8384302
1993 Rabphilin-3A has two functionally distinct domains: an N-terminal domain (residues 1–280) that binds GTP-Rab3A and a C-terminal domain (residues 281–704) containing two C2 domains that bind phospholipid and Ca2+ in a cooperative manner. In vitro binding assays using recombinant full-length, N-terminal, and C-terminal fragments of rabphilin-3A with GTP-Rab3A, phospholipid, and 45Ca2+ The Journal of biological chemistry High 8262955
1993 Rabphilin-3A and its N-terminal fragment inhibit Rab3A GAP-stimulated GTPase activity of Rab3A, thereby potentially keeping Rab3A in the GTP-bound active form. In vitro GTPase assay measuring basal and GAP-stimulated GTP hydrolysis of Rab3A in the presence of rabphilin-3A or its fragments The Journal of biological chemistry High 8226731
1994 Rabphilin-3A is localized on synaptic vesicles in presynaptic terminals, as determined by immunogold electron microscopy of neuromuscular junctions and subcellular fractionation showing enrichment in purified synaptic vesicle fractions. Immunogold electron microscopy and subcellular fractionation of rat brain Biochemical and biophysical research communications High 8060298
1994 Synaptic targeting of rabphilin-3A depends on Rab3A/3C: in rab3A-deficient mice, rabphilin-3A is decreased at synapses of neurons expressing primarily rab3A and accumulates in perikarya, while neurons expressing rab3C retain normal synaptic rabphilin-3A; rabphilin-3A binds rab3C in vitro. Immunofluorescence and Western blot in rab3A knockout mice; in vitro binding assay with rab3C Neuron High 7946335
1994 Rabphilin-3A is associated with synaptic vesicles via a vesicle-resident protein in a manner independent of Rab3A; removal of Rab3A or addition of exogenous Rab3A did not affect rabphilin-3A binding to vesicles, but trypsin treatment abolished binding. Reconstitution binding assay using salt-stripped synaptic vesicles, Rab GDI extraction of endogenous Rab3A, trypsin treatment The Journal of biological chemistry Medium 7806490
1994 Rabphilin-3A is phosphorylated by cyclic AMP-dependent protein kinase (PKA) at its N-terminal region (~0.8 mol phosphate/mol protein), identifying it as a PKA substrate involved in neurotransmitter release regulation. In vitro phosphorylation assay with purified recombinant rabphilin-3A and PKA Biochemical and biophysical research communications High 7945346
1994 CaMKII phosphorylates rabphilin-3A at Ser34, Thr205, Thr209, and Thr537 (two mol phosphate maximally incorporated), identifying additional regulatory phosphorylation sites. In vitro phosphorylation assay with CaMKII purified from rat brain and recombinant rabphilin-3A; phosphorylation site identification Biochemical and biophysical research communications Medium 7811264
1994 Rabphilin-3A binds beta-adducin through its C-terminal C2 domain-containing region in the presence of Ca2+ and phosphatidylserine, identifying beta-adducin as a calcium/phospholipid-dependent binding partner. Purification and amino acid sequence analysis of co-purified 115 kDa protein; overlay assay and biochemical characterization Biochemical and biophysical research communications Medium 7999065
1994 GTP cyclohydrolase I (Mr ~30 kDa) co-immunoprecipitates with rabphilin-3A from PC12 cell lysates and is phosphorylated upon high KCl stimulation, identifying it as a rabphilin-3A-interacting protein in neuroendocrine cells. Co-immunoprecipitation from PC12 cell lysate followed by SDS-PAGE and amino acid sequence analysis Biochemical and biophysical research communications Low 7802677
1994 Rabphilin-3A has GDP/GTP exchange activity for Rab3A, potentially keeping Rab3A in the GTP-bound form by re-converting GDP-Rab3A produced by GAP activity. In vitro GDP/GTP exchange assay with purified Rab3A and rabphilin-3A FEBS letters Medium 7926025
1995 Rabphilin-3A is phosphorylated by CaMKII at residues 234 and 274 and by cAMP-dependent protein kinase (PKA) at residue 234, placing the middle region between the Rab3A-binding domain and C2 domains as a convergent regulatory phosphorylation site. In vitro phosphorylation assays with CaMKII and PKA; tryptic peptide mapping to identify phosphorylation sites The Journal of neuroscience High 7891174
1996 Rabphilin-3A dissociates from synaptic vesicles after exocytosis in a manner requiring Ca2+ and membrane fusion; it interacts with GTP-Rab3A via an N-terminal Zn2+-finger motif, and this interaction is essential for rabphilin binding to synaptic vesicles, showing that Rab3 reversibly recruits rabphilin to vesicles analogously to Ras recruiting Raf. Exocytosis-coupled dissociation assay from synaptic vesicles; Zn2+-finger domain mutational analysis; in vitro binding The EMBO journal High 8617225
1996 Alpha-actinin interacts with the N-terminal region of rabphilin-3A (the same region that binds GTP-Rab3A), and this interaction stimulates alpha-actinin's ability to cross-link actin filaments into bundles; GTPγS-Rab3A inhibits the rabphilin-3A/alpha-actinin interaction. Yeast two-hybrid screen from human brain cDNA library; direct binding assays; actin cross-linking assay The Journal of biological chemistry High 8943213
1996 Rabphilin-3A is involved in Ca2+-dependent exocytosis from PC12 cells: reduction of endogenous rabphilin-3A inhibits high K+-induced growth hormone release, and N-terminal, C-terminal, or C2B fragments (but not C2A fragment alone) inhibit release when overexpressed. Growth hormone co-expression assay in PC12 cells; antisense knockdown; overexpression of domain fragments Biochemical and biophysical research communications Medium 8605005
1996 The Cys-rich region in the N-terminal domain binds two Zn2+ ions and is necessary but not sufficient for efficient Rab3A binding; a minimal Rab3A-binding domain spans residues 45–170; C2 domains are required for efficient membrane attachment in PC12 cells; Rab3A binding targets rabphilin to the correct membrane compartment. Deletion and mutagenesis analysis; Rab3A binding assays; GFP-rabphilin subcellular localization in PC12 cells; zinc binding characterization Molecular and cellular biology Medium 8756657
1998 Presynaptic microinjection of rabphilin-3A into squid giant synapse reversibly inhibits neurotransmitter release; the N-terminal Rab3A-binding/phosphorylation region and the two C2 domains each independently inhibit release; the N-terminal domain also perturbs endocytosis (altering endosome, coated vesicle, and plasma membrane areas). Presynaptic microinjection of recombinant rabphilin-3A and domain fragments into squid giant synapse; electrophysiology; electron microscopy The Journal of general physiology High 9450942
1998 Rabphilin phosphorylation at Ser234 is selectively increased by PKA and Ca2+ influx in mossy fiber CA3 synaptosomes but not in CA1 synaptosomes, correlating with the region-specific PKA-dependent LTP in mossy fibers. Phosphorylation assay in isolated CA1 and CA3 synaptosomes with forskolin treatment and K+-depolarization; region-specific fractionation of hippocampus The Journal of neuroscience Medium 9425005
1999 Crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A solved at 2.6 Å resolution; rabphilin-3A contacts Rab3A at two interfaces: switch I/II regions (nucleotide-state sensitive) and a deep pocket (RabCDR) containing the SGAWFF element of rabphilin-3A that confers Rab effector specificity. X-ray crystallography at 2.6 Å resolution; biochemical binding data Cell High 10025402
1999 NMR solution structure of the C2B domain of rabphilin-3A reveals a Janus-faced architecture: a Ca2+-binding top surface (similar to other C2 domains) and a Ca2+-independent bottom surface bearing a conserved alpha-helix, suggesting Ca2+-independent protein interactions from the bottom surface. NMR spectroscopy structure determination Nature cell biology High 10559882
2001 High-affinity Rab3 binding is dispensable for rabphilin's stimulatory effect on Ca2+-regulated secretion in HIT-T15 cells; mutations V61A and L83A abolish Rab3 binding but do not impair secretory potentiation; however, mutant R60A retains Rab3 binding but loses stimulatory activity, suggesting secretory potentiation depends on an unknown factor binding to the Rab3-binding domain. Point mutagenesis of Rab3-binding domain; secretion assay in HIT-T15 cells; in vitro Rab3 binding assay Journal of cell science Medium 10504306
2001 Rabphilin promotes receptor-mediated endocytosis through interaction with Rabaptin-5; this activity is negatively regulated by Rab3. The Rabphilin V61A mutant (unable to bind Rab3) enhances transferrin endocytosis and binds Rabaptin-5, whereas L83A (also Rab3-binding defective) does not interact with Rabaptin-5 and does not enhance endocytosis. Transferrin endocytosis assay; co-immunoprecipitation of Rabaptin-5; Rab3-binding mutant analysis in HIT-T15 cells Journal of cell science Medium 11309205
2001 In C. elegans, rabphilin mutants show synergistic genetic interactions with hypomorphic SNARE (syntaxin, SNAP-25, synaptobrevin) mutants, causing severe behavioral defects not seen in rab3-SNARE double mutants, establishing a Rab3-independent function of rabphilin in potentiating SNARE function. C. elegans genetic epistasis; behavioral assays (locomotion, mechanosensation); pharmacological assays The Journal of neuroscience High 11717359
2001 Phosphorylation of rabphilin on Ser234 and Ser274 is dynamically regulated in nerve terminals; soluble rabphilin (not vesicle-bound) is the primary phosphorylation target; phospho-rabphilin shows reduced membrane affinity; phosphorylation requires external Ca2+ and Rab3A; PKA and CaMKII differentially phosphorylate the two sites. Phospho-specific antibodies against Ser234 and Ser274; subcellular fractionation; synaptosome stimulation experiments; depolarization assays The Journal of neuroscience High 11466418
2003 Rabphilin interacts with Rab3A/B/C/D, Rab8A, and Rab27A/B (but not other Rabs), and Noc2 shows the same specificity; this broader Rab specificity beyond Rab3 suggests rabphilin functions as effector for multiple Rab proteins including Rab27. Co-transfection assay testing rabphilin binding to 42 different Rab proteins; site-directed mutagenesis of Rab-binding domain The Journal of biological chemistry Medium 12578829
2004 Rabphilin is recruited to dense-core vesicles in PC12 cells through specific interaction with Rab27A, not Rab3A; Rab3A-binding-defective mutant (E50A) still localizes to vesicles, but Rab27A-binding-defective double mutant (E50A/I54A) is cytosolic; this is conserved across C. elegans and Drosophila orthologs. Deletion and mutation analysis; live-cell localization in PC12 cells; neuropeptide Y secretion assay; cross-species binding analysis The Journal of biological chemistry High 14722103
2004 The C2B domain of rabphilin directly interacts with annexin A4; rabphilin, annexin A4, and synaptotagmin 1 form a novel protein complex in PC12 cells; annexin A4 colocalizes with rabphilin at the plasma membrane. Yeast two-hybrid; pulldown assay; co-immunoprecipitation from PC12 cells; immunofluorescence colocalization FEBS letters Medium 14960300
2005 The C2B domain of rabphilin directly interacts with the plasma membrane SNARE protein SNAP-25 (EC50 ~0.82 μM; Ca2+ increases affinity ~2-fold); rabphilin expression increases the number of docked dense-core vesicles at the plasma membrane in PC12 cells without altering fusion kinetics; rabphilin-ΔC2B decreases docked vesicles. In vitro binding assay (SNAP-25 interaction); TIRFM of neuropeptide Y-Venus in PC12 cells; C2B domain mutagenesis The Journal of biological chemistry High 16203731
2005 Rabphilin localizes to the subplasmalemmal actin cytoskeleton via an alpha-actinin-dependent mechanism; purified rabphilin associates with F-actin only in the presence of alpha-actinin; rabphilin stimulates (~8-fold) the association of granules with alpha-actinin-crosslinked F-actin in an in vitro assay. Immunofluorescence and immunoelectron microscopy; in vitro F-actin binding assay with purified components; granule-actin co-sedimentation assay The Journal of biological chemistry Medium 16043482
2006 Deletion of rabphilin dramatically accelerates recovery of synaptic vesicle pools from use-dependent depression; this phenotype is rescued by wild-type rabphilin but not by rabphilin lacking the C2B domain (which binds SNAP-25); double deletion of rabphilin and synaptobrevin further increases responses from depleted pools, suggesting rabphilin regulates SNARE-dependent re-priming via C2B-SNAP-25 interaction. Electrophysiological recordings in rabphilin knockout neurons; viral rescue with wild-type and C2B-mutant rabphilin; synaptobrevin double knockout analysis The EMBO journal High 16763567
2006 The polybasic sequence (587KKAKHKTQIKKK598) in the C2B domain of rabphilin is required for SNAP-25 binding; Lys→Gln mutations abolishing SNAP-25 binding significantly decrease plasma-membrane-docked vesicles and inhibit high-KCl-induced DCV exocytosis in PC12 cells. SNAP-25 binding assay with polybasic sequence mutants; TIRFM of PC12 cells; high-KCl exocytosis assay Journal of neurochemistry High 17156129
2006 The crystal structure of the Ca2+-free C2A domain of rabphilin-3A was solved at 1.92 Å; the domain adopts a classical eight-stranded antiparallel beta-sandwich with conserved Ca2+-binding acidic residues; a conserved Asp→Glu substitution increases rigidity of Ca2+-binding loop 1. X-ray crystallography at 1.92 Å resolution Acta crystallographica. Section D, Biological crystallography High 16790935
2006 Acidic residues from the C2A-C2B linker interact with the Ca2+-binding region of the C2B domain, providing an unusual Ca2+-binding mode; mutation of these linker residues to Ala caused a 10-fold decrease in C2B Ca2+-binding affinity; this interaction persists in the full C2 domain tandem. X-ray crystallography of C2B domain with linker; NMR spectroscopy; Ca2+-binding affinity measurements; site-directed mutagenesis The Journal of biological chemistry High 17166855
2008 Both C2A and C2B domains of rabphilin-3A bind PIP2; C2A binds the PIP2 headgroup IP3 in a Ca2+-dependent manner (Kd ~55 μM at saturating Ca2+) via a defined binding site on the concave surface, with IP3 and Ca2+ mutually enhancing binding (TAMA mechanism); C2B binds IP3 in a Ca2+-independent fashion with low affinity via a different binding mode. NMR spectroscopy mapping of IP3 binding to each C2 domain; Ca2+-binding affinity measurements Protein science High 18434502
2008 The Ca2+-bound solution structure of the C2A domain shows Ca2+ induces a conformational change in Ca2+-binding loop 3 (CBL3) that enables IP3 binding; the structural basis for the TAMA (target-activated messenger affinity) mechanism was determined. NMR solution structure determination of Ca2+-bound C2A domain; IP3 docking model; mutagenesis of CBL3 The Journal of biological chemistry High 18945677
2008 Rabphilin (as Rab27 effector) undergoes rapid and complete exchange between secretory granules and cytosol in PC12 cells (as measured by FRAP), unlike Granuphilin and Noc2 which show little exchange; both Noc2 and Rabphilin are recruited to granules by Rab27 but Rabphilin does not form stable Rab27 complexes on granules. FRAP (fluorescence recovery after photobleaching) of EGFP-tagged Rabphilin, Granuphilin, and Noc2 in PC12 cells Biochemical and biophysical research communications Medium 18573236
2015 Rabphilin 3A (Rph3A) is enriched at dendritic spines (postsynaptic site) and forms a ternary complex with GluN2A (binding to GluN2A residues 1349–1389) and PSD-95 (PDZ3 domain) via its N-terminal domain; Rph3A silencing reduces surface localization of synaptic GluN2A and NMDAR currents; interfering peptides disrupting GluN2A/Rph3A interaction decrease NMDAR-mediated currents and GluN2A density at dendritic spines. Protein-protein interaction assays (co-IP, GST pulldown); RNAi knockdown in neurons; whole-cell patch clamp; confocal imaging; in vivo peptide injection in organotypic slices Nature communications High 26679993
2017 Crystal structures of rabphilin-3A C2B domain bound to SNAP-25 and to PIP2 were solved; rabphilin-3A C2B uses a unique structural element (bottom alpha-helix) to contact the same SNAP-25 surface as synaptotagmin-1; the C2B domain can simultaneously bind PIP2/Ca2+ and SNAP-25, adopting a conformation compatible with interaction with the complete SNARE complex and suggesting membrane bending in Ca2+-dependent fusion. X-ray crystallography of C2B-SNAP25 and C2B-PIP2 complexes; biochemical binding analyses Proceedings of the National Academy of Sciences of the United States of America High 28634303
2017 PKN1 phosphorylates RPH3A, which enhances binding of RPH3A to GTP-bound RAB21; PKN1 and RPH3A are required for polarized localization of RAB21 and RPH3A in neutrophils, leading to PIP5K1C90 polarization essential for integrin activation and neutrophil adhesion to endothelial cells. Kinase assay; co-IP; confocal imaging of neutrophil polarization; siRNA knockdown; inflammatory adhesion assay; myeloid-specific PKN1 knockout mice Cell reports High 28636945
2018 A missense variant in RPH3A (p.Arg269Gln) strongly impairs binding of rabphilin-3A to 14-3-3 protein; this variant is associated with presynaptic congenital myasthenic syndrome with altered synaptic vesicle homeostasis. Expression studies in mammalian cell lines; 14-3-3 binding assay comparing wild-type and mutant rabphilin-3A Molecular genetics & genomic medicine Medium 29441694
2020 ARF6 binds RPH3A and enhances the interaction between plasma membrane PtdIns4P and RPH3A; ARF6 polarization at the plasma membrane coincides with RPH3A, RAB21, PIP5K1C90, and PM PtdIns4P in neutrophils upon integrin stimulation; ARF6 functions as a coincidence-detection code directing RPH3A polarization. siRNA knockdown of ARF6; dominant-negative ARF6 mutant; co-immunoprecipitation; confocal imaging of neutrophil polarization; ARF6 inhibitor SecinH3 Journal of immunology Medium 31924649
2021 UBE3A mono-ubiquitinates RPH3A in mouse brain via a non-degradative mechanism; the UBE3A and RAB3A binding sites on RPH3A partially overlap, and RAB3A binding interferes with UBE3A binding; reduced RPH3A levels in absence of RAB3A are not mediated by UBE3A; an AS-linked UBE3A missense mutation abrogates interaction with RPH3A. Co-immunoprecipitation; ubiquitination assays in mouse brain; RAB3A knockout comparison; UBE3A missense variant binding assay Scientific reports Medium 33542309
2022 Rabphilin-3A interacts with alpha-synuclein; in vivo intrastriatal injection of alpha-synuclein preformed fibrils reduces synaptic levels of Rph3A and impairs Rph3A/NMDAR interaction; restoring Rph3A expression or disrupting the Rph3A/alpha-synuclein complex prevents dendritic spine loss and rescues early motor defects. In vivo intrastriatal injection; Western blotting; co-immunoprecipitation; confocal imaging; motor behavioral testing; in vitro primary neuron experiments with small molecule Pharmacological research Medium 35918045
2022 Rabphilin-3A promotes SNARE complex assembly by binding the N-peptide region of SNAP-25 via the C2B bottom alpha-helix; this interaction is enabled by an intramolecular interplay between the N-terminal Rab-binding domain and the C-terminal C2AB domain; the C2B/SNAP-25 N-peptide interaction induces a conformational switch from random coils to alpha-helical structure in the SNAP-25 SNARE motif, accelerating SNARE complex assembly; disrupting this interaction impairs vesicle docking and fusion in PC12 cells. In vitro SNARE complex assembly assay; NMR; binding studies; mutagenesis; TIRF microscopy in PC12 cells eLife High 36173100
2022 Rph3A overexpression in hippocampal neurons increases dendritic spine density, increases synaptic GluN2A-containing NMDARs, and decreases surface GluA1-containing AMPARs; these changes occlude LTP-induced spine formation but do not prevent LTD-induced spine loss. Confocal imaging of spine density; surface NMDAR and AMPAR quantification; LTP and LTD induction protocols in primary hippocampal neurons Cells Medium 35626653
2023 Rph3A undergoes liquid-liquid phase separation dependent on arginine residues in its N-terminal domain; phase separation promotes GluN2A clustering by binding GluN2A C-terminal domain; a ternary Rph3A/GluN2A/PSD95 complex promotes Rph3A phase separation; disrupting Rph3A phase separation suppresses synaptic and extrasynaptic surface clustering, synaptic localization, and stability of GluN2A and reduces NMDAR synaptic responses. In vitro phase separation assay; mutagenesis of arginine residues; FRAP; super-resolution imaging; electrophysiology in hippocampal neurons Nature communications High 36693856
2023 RPH3A missense gain-of-function variants (p.Thr450Ser, p.Asn618Ser) reduce synaptic localization of GluN2A; p.Thr450Ser also increases surface GluN2A levels; both variants increase GluN2A-dependent NMDAR currents and alter postsynaptic calcium levels; Thr450Ser expression affects dendritic spine morphology. Primary hippocampal neuronal cultures; immunofluorescence; whole-cell patch clamp; calcium imaging; spine morphology analysis Genetics in medicine Medium 37403762
2018 CAND1 (cullin-associated NEDD8-dissociated protein 1) was identified as a rabphilin-3A-binding protein in posterior pituitary by GST-pulldown and co-immunoprecipitation; CAND1 overexpression leads to deubiquitylation of rabphilin-3A in PC12 cells and enhances both basal and KCl-stimulated AVP secretion. GST-pulldown; proteomic analysis; co-immunoprecipitation; ubiquitination assay; AVP secretion assay in PC12 cells Endocrine journal Medium 29367474
2024 RPH3A is a negative regulator of dense-core vesicle (DCV) exocytosis in hippocampal neurons; RPH3A KO increases DCV exocytosis ~3-fold; RAB3A-binding deficient RPH3A loses synaptic enrichment but still restores WT DCV exocytosis; SNAP25-binding deficient RPH3A does not rescue DCV exocytosis, indicating SNAP25 interaction mediates the inhibitory function; RPH3A resides stationary at presynapses and does not travel with DCVs. Live-cell imaging at single-vesicle resolution in RPH3A knockout hippocampal neurons; viral re-expression of wild-type and mutant RPH3A; tetanus neurotoxin ablation of regulated secretion eLife High 39412498
2025 RPH3A variants p.(Arg209Lys) and p.(Gln508His) reduce presynaptic glutamate release and decrease synaptic retention of GluN2A-containing NMDARs, with reduced calcium event frequency at dendritic spines, demonstrating both pre- and post-synaptic dysfunction from disease-associated RPH3A missense variants. Primary hippocampal neuronal cultures; electrophysiology; calcium imaging; immunofluorescence of GluN2A synaptic localization Scientific reports Medium 40082528

Source papers

Stage 0 corpus · 90 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1993 Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin. Molecular and cellular biology 378 8384302
1999 Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell 299 10025402
1994 Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron 183 7946335
2003 Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. The Journal of biological chemistry 159 12578829
1993 Two functionally different domains of rabphilin-3A, Rab3A p25/smg p25A-binding and phospholipid- and Ca(2+)-binding domains. The Journal of biological chemistry 136 8262955
1996 Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras. The EMBO journal 121 8617225
1994 Localization of Rabphilin-3A on the synaptic vesicle. Biochemical and biophysical research communications 104 8060298
1999 Novel rabphilin-3-like protein associates with insulin-containing granules in pancreatic beta cells. The Journal of biological chemistry 101 10497219
1996 Physical and functional interaction of rabphilin-3A with alpha-actinin. The Journal of biological chemistry 101 8943213
1995 Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. The Journal of neuroscience : the official journal of the Society for Neuroscience 98 7891174
1998 Rabphilin-3A: a multifunctional regulator of synaptic vesicle traffic. The Journal of general physiology 94 9450942
2006 Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion. The EMBO journal 85 16763567
2005 The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. The Journal of biological chemistry 82 16203731
2004 Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. The Journal of biological chemistry 81 14722103
1999 Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling. The Journal of biological chemistry 81 10464283
2007 Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease. Journal of neurochemistry 69 17877635
1998 Region-specific phosphorylation of rabphilin in mossy fiber nerve terminals of the hippocampus. The Journal of neuroscience : the official journal of the Society for Neuroscience 69 9425005
1999 Structure of the Janus-faced C2B domain of rabphilin. Nature cell biology 65 10559882
1993 Rab3A GTPase-activating protein-inhibiting activity of Rabphilin-3A, a putative Rab3A target protein. The Journal of biological chemistry 63 8226731
2015 Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nature communications 62 26679993
1994 Rabphilin-3A is associated with synaptic vesicles through a vesicle protein in a manner independent of Rab3A. The Journal of biological chemistry 61 7806490
1996 Role of the Rab3A-binding domain in targeting of rabphilin-3A to vesicle membranes of PC12 cells. Molecular and cellular biology 54 8756657
2001 Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs. The Journal of biological chemistry 53 11431472
2010 A manual collection of Syt, Esyt, Rph3a, Rph3al, Doc2, and Dblc2 genes from 46 metazoan genomes--an open access resource for neuroscience and evolutionary biology. BMC genomics 47 20078875
2003 Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. The American journal of pathology 47 12937130
1996 Involvement of rabphilin-3A in Ca2+-dependent exocytosis from PC12 cells. Biochemical and biophysical research communications 46 8605005
1994 Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells. Biochemical and biophysical research communications 45 7802677
1996 Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs. The Journal of cell biology 44 8991087
2008 The PIP2 binding mode of the C2 domains of rabphilin-3A. Protein science : a publication of the Protein Society 42 18434502
2001 Rabphilin potentiates soluble N-ethylmaleimide sensitive factor attachment protein receptor function independently of rab3. The Journal of neuroscience : the official journal of the Society for Neuroscience 42 11717359
2013 Decreased rabphilin 3A immunoreactivity in Alzheimer's disease is associated with Aβ burden. Neurochemistry international 40 24200817
1994 Identification as beta-adducin of a protein interacting with rabphilin-3A in the presence of Ca2+ and phosphatidylserine. Biochemical and biophysical research communications 37 7999065
2017 Structural characterization of the Rabphilin-3A-SNAP25 interaction. Proceedings of the National Academy of Sciences of the United States of America 36 28634303
2017 Rabphilin 3A: A novel target for the treatment of levodopa-induced dyskinesias. Neurobiology of disease 36 28823933
2008 Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A. The Journal of biological chemistry 33 18945677
1994 Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II. Biochemical and biophysical research communications 33 7811264
1999 High affinity Rab3 binding is dispensable for Rabphilin-dependent potentiation of stimulated secretion. Journal of cell science 31 10504306
2017 PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury. Cell reports 30 28636945
2005 Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction. Neurobiology of disease 30 15967669
1997 The stimulatory effect of rabphilin 3a on regulated exocytosis from insulin-secreting cells does not require an association-dissociation cycle with membranes mediated by Rab 3. European journal of cell biology 28 9402469
1994 Phosphorylation of Rabphilin-3A, a putative target protein for Rab3A, by cyclic AMP-dependent protein kinase. Biochemical and biophysical research communications 28 7945346
1994 Cloning of a mouse Rabphilin-3A expressed in hormone-secreting cells. Journal of biochemistry 27 7822236
2018 Critical role of rabphilin-3A in the pathophysiology of experimental lymphocytic neurohypophysitis. The Journal of pathology 25 29377134
2001 Physiological modulation of rabphilin phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience 23 11466418
2019 Protein and fat intake interacts with the haplotype of PTPN11_rs11066325, RPH3A_rs886477, and OAS3_rs2072134 to modulate serum HDL concentrations in middle-aged people. Clinical nutrition (Edinburgh, Scotland) 22 31006500
2001 Rabphilin dissociated from Rab3 promotes endocytosis through interaction with Rabaptin-5. Journal of cell science 22 11309205
2006 The polybasic sequence in the C2B domain of rabphilin is required for the vesicle docking step in PC12 cells. Journal of neurochemistry 21 17156129
2000 Physical and functional interaction of rabphilin-11 with mammalian Sec13 protein. Implication in vesicle trafficking. The Journal of biological chemistry 21 10747849
2022 Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease. Pharmacological research 20 35918045
2006 Deletion of synapsins I and II genes alters the size of vesicular pools and rabphilin phosphorylation. Brain research 19 16844103
2022 Role of Rph3A in brain injury induced by experimental cerebral ischemia-reperfusion model in rats. CNS neuroscience & therapeutics 18 35467084
2008 The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells. Biochemical and biophysical research communications 18 18573236
2004 Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin. FEBS letters 18 14960300
1994 Localization of rabphilin-3A, a putative target protein for Rab3A, at the sites of Ca(2+)-dependent exocytosis in PC12 cells. Biochemical and biophysical research communications 18 8292018
2011 Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer. Clinica chimica acta; international journal of clinical chemistry 17 21536019
2005 Rabphilin localizes with the cell actin cytoskeleton and stimulates association of granules with F-actin cross-linked by {alpha}-actinin. The Journal of biological chemistry 17 16043482
2006 The C2A-C2B linker defines the high affinity Ca(2+) binding mode of rabphilin-3A. The Journal of biological chemistry 16 17166855
1995 Rabphilin-3A binds to a M(r) 115,000 polypeptide in a phosphatidylserine- and Ca(2+)-dependent manner. Brain research. Molecular brain research 16 7707875
2020 Small GTPase ARF6 Is a Coincidence-Detection Code for RPH3A Polarization in Neutrophil Polarization. Journal of immunology (Baltimore, Md. : 1950) 15 31924649
2023 Rabphilin-3A undergoes phase separation to regulate GluN2A mobility and surface clustering. Nature communications 13 36693856
2018 Presynaptic congenital myasthenic syndrome with altered synaptic vesicle homeostasis linked to compound heterozygous sequence variants in RPH3A. Molecular genetics & genomic medicine 13 29441694
1998 Depolarization of cerebellar granule cells increases phosphorylation of rabphilin-3A. Journal of neurochemistry 13 9751200
1994 GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein. FEBS letters 13 7926025
2023 Missense variants in RPH3A cause defects in excitatory synaptic function and are associated with a clinically variable neurodevelopmental disorder. Genetics in medicine : official journal of the American College of Medical Genetics 12 37403762
2001 Characterization of rabphilin phosphorylation using phospho-specific antibodies. Neuropharmacology 12 11640918
2021 Mono-ubiquitination of Rabphilin 3A by UBE3A serves a non-degradative function. Scientific reports 10 33542309
2022 Rabphilin-3A Drives Structural Modifications of Dendritic Spines Induced by Long-Term Potentiation. Cells 9 35626653
2008 In-depth fluorescence lifetime imaging analysis revealing SNAP25A-Rabphilin 3A interactions. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 9 18986604
2022 A 7-year-old boy with central diabetes insipidus presenting with thickened pituitary stalk and anti-rabphilin-3A antibody positivity. Journal of pediatric endocrinology & metabolism : JPEM 8 35119230
2022 Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis. eLife 8 36173100
2020 Rabphilin involvement in filtration and molecular uptake in Drosophila nephrocytes suggests a similar role in human podocytes. Disease models & mechanisms 8 32680845
2009 Immunohistochemical expression of rabphilin-3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas. Biotechnic & histochemistry : official publication of the Biological Stain Commission 7 19212825
2024 Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease. Scientific reports 6 39134564
2023 Two children with lymphocytic hypophysitis presenting with positive anti-rabphilin-3A antibody. Endocrine journal 6 37045780
2021 Ca2+ sensor proteins in spontaneous release and synaptic plasticity: Limited contribution of Doc2c, rabphilin-3a and synaptotagmin 7 in hippocampal glutamatergic neurons. Molecular and cellular neurosciences 6 33753311
2021 Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage. Scientific reports 6 34315987
2006 Structure of the C2A domain of rabphilin-3A. Acta crystallographica. Section D, Biological crystallography 6 16790935
2005 Assay of the Rab-binding specificity of rabphilin and Noc2: target molecules for Rab27. Methods in enzymology 6 16473612
2018 Cullin-associated NEDD8-dissociated protein 1, a novel interactor of rabphilin-3A, deubiquitylates rabphilin-3A and regulates arginine vasopressin secretion in PC12 cells. Endocrine journal 5 29367474
2025 Arg209Lys and Gln508His missense variants in Rabphilin 3A cause pre- and post-synaptic dysfunctions at excitatory glutamatergic synapses. Scientific reports 4 40082528
2020 The Rab-Rabphilin system in injured human podocytes stressed by glucose overload and angiotensin II. American journal of physiology. Renal physiology 4 32567349
2008 Detection of the interaction between SNAP25 and rabphilin in neuroendocrine PC12 cells using the FLIM/FRET technique. Microscopy research and technique 4 17886343
2024 Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction. eLife 3 39412498
2023 Absence of anti-rabphilin-3A antibodies in children and young adults with idiopathic central diabetes insipidus: a potential clue to elucidating a tumor etiology. Hormones (Athens, Greece) 3 37697216
2022 Lymphocytic panhypophysitis and anti-rabphilin-3A antibody with pulmonary sarcoidosis. Pituitary 3 35088194
2022 Central diabetes insipidus with anti-rabphilin-3A antibody positivity causing hypovolemic shock after resection of tumorous lesions in the pelvic cavity. CEN case reports 2 36574196
1995 Heterotetramer formation of prenylated Rab3A with two Rabphilin-3A molecules. Biochemical and biophysical research communications 2 8554624
2024 A 4-year-old Boy Positive for Anti-rabphilin-3A Antibody and Diagnosed With Lymphocytic Infundibuloneurohypophysitis. JCEM case reports 1 39726668
2006 Tyrosine phosphorylation of rabphilin during long-lasting long-term potentiation. Neuroscience letters 1 17223267
2025 Lymphocytic panhypophysitis with clival extension and positive anti-rabphilin-3A antibodies: illustrative case. Journal of neurosurgery. Case lessons 0 41569905

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