Affinage

PTBP1

Polypyrimidine tract-binding protein 1 · UniProt P26599

Length
557 aa
Mass
59.6 kDa
Annotated
2026-04-28
100 papers in source corpus 44 papers cited in narrative 44 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PTBP1 is a polypyrimidine tract-binding protein that functions as a master post-transcriptional regulator, controlling alternative splicing, mRNA stability, translation, and miRNA biogenesis across diverse developmental and disease contexts. Its splicing activity follows positional rules: PTBP1-mediated RNA loops within individual introns promote cassette exon inclusion by accelerating asymmetric intron removal, whereas loops spanning across exons repress inclusion (PMID:36958328). PTBP1 directs metabolic reprogramming through PKM2/PKM1 isoform switching—a process enhanced by KAT2B-mediated crotonylation at K266, which strengthens PTBP1–hnRNPA1/A2 interaction (PMID:32497094, PMID:39497094)—and its protein stability is regulated by lactylation at K436 (written metabolically, erased by SIRT1, antagonizing TRIM21-mediated ubiquitination) and by the deubiquitinases PSMD14 and USP46 (PMID:39570804, PMID:35405117, PMID:38176460). Beyond splicing, PTBP1 destabilizes specific mRNAs (MCL1, SLC31A1, USP8), enhances translation of others (CCND3), suppresses let-7 miRNA maturation, and is recruited to pre-mRNA targets by lncRNA scaffolds including MALAT1, LUCAT1, and DCRT; conditional knockouts reveal essential roles in neuronal differentiation, cardiac morphogenesis (left ventricular noncompaction upon endothelial deletion), endothelial NF-κB priming and atherosclerosis, B-cell activation, and axonal regeneration after nerve injury (PMID:27367564, PMID:32207235, PMID:30778047, PMID:36563164, PMID:38841852, PMID:37002228, PMID:35858420, PMID:30476084, PMID:37506203).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1997 Medium

    Before domain-function mapping, how PTBP1 reached the nucleus was unknown; identification of a novel 60-amino-acid bipartite NLS in the N-terminus established that PTBP1 uses a non-canonical mechanism for nuclear import.

    Evidence CAT reporter fusion constructs and transient transfection localization assays

    PMID:9281380

    Open questions at the time
    • No importin-binding partner identified
    • NLS regulation by PTMs not tested
    • Shuttling mechanism between nucleus and cytoplasm not addressed
  2. 2011 Medium

    The question of whether PTBP1 splicing regulation has functional consequences in cancer was addressed when PTBP1 was shown to control USP5 isoform switching in glioblastoma, with knockdown inhibiting tumor cell growth and migration.

    Evidence Exon arrays, siRNA knockdown, antisense oligonucleotides, cell growth/migration assays in GBM cells

    PMID:21976412

    Open questions at the time
    • Direct binding site on USP5 pre-mRNA not mapped at nucleotide resolution
    • No in vivo GBM model tested
  3. 2015 High

    How PTBP1 controls cell fate decisions during neuronal commitment was clarified by showing it represses Pbx1 exon 7 in ESCs, suppressing the neuronal Pbx1a transcriptional isoform; CRISPR deletion of regulatory elements activated neuronal gene programs.

    Evidence CRISPR-Cas9 regulatory element deletion, splicing and transcriptional reporter assays in ESCs/NPCs

    PMID:26705333

    Open questions at the time
    • Downstream Pbx1a transcriptional targets not fully catalogued
    • Whether other RBPs compensate in Pbx1 regulation not tested
  4. 2016 High

    A central question was whether PTBP1 and PTBP2 achieve distinct splicing outputs through different RNA-binding preferences or different cofactor usage; knockin replacement showed near-identical binding profiles but distinct splicing outcomes, establishing cofactor interactions as the primary basis for paralog specificity.

    Evidence Conditional knockin mouse replacing Ptbp2 with Ptbp1, transcriptome-wide eCLIP/RNA-seq comparison

    PMID:27926877

    Open questions at the time
    • Specific cofactors responsible for differential outcomes not identified in this study
    • Structural basis of differential cofactor recruitment unknown
  5. 2016 Medium

    PTBP1's role in metabolic reprogramming was established when it was shown to promote PKM2 isoform expression via alternative splicing, driving the Warburg effect; miR-1 and miR-133b antagonize this by silencing PTBP1.

    Evidence Ectopic miRNA expression, PKM isoform RT-PCR, in vitro and in vivo growth assays in CRC

    PMID:26980745

    Open questions at the time
    • Precise PTBP1 binding sites on PKM pre-mRNA not mapped
    • Contribution of cofactors to PKM splicing not dissected
  6. 2018 Medium

    Whether PTBP1 acts beyond splicing to control mRNA translation was answered by showing it binds the CCND3 5'-UTR and enhances translation, promoting cell cycle progression in HCC; separately, it was shown to facilitate miR-101-guided AGO2 targeting of MCL1 mRNA, revealing coordination with miRISC.

    Evidence RIP, polysome fractionation, 5'-UTR luciferase reporter (CCND3); AGO2-RIP and functional co-knockdown (MCL1/miR-101)

    PMID:29748555 PMID:31301177

    Open questions at the time
    • Structural basis of PTBP1-mediated translational enhancement not resolved
    • Generality of PTBP1–miRISC cooperation across other targets unknown
  7. 2018 Medium

    Mass spectrometry revealed that PTBP1 carries distinct phosphorylation and acetylation marks compared to PTBP2 under splicing-active conditions, suggesting PTMs as an additional layer of paralog-specific regulation.

    Evidence Mass spectrometry of PTMs on proteins from in vitro splicing reactions

    PMID:29851470

    Open questions at the time
    • Functional consequence of individual PTM sites not tested by mutagenesis in this study
    • Writers and erasers for most sites not identified
  8. 2019 Medium

    Multiple studies in 2019 expanded the catalog of PTBP1 splicing targets and cofactors: NOVA1 recruits PTBP1 to hTERT pre-mRNA to maintain telomerase activity; PTBP1 cooperates with hnRNPH1 to regulate TCF3 mutually exclusive exon selection via intronic silencers; PTBP1 modulates CD33 exon 2 inclusion at an Alzheimer's-associated SNP; and PTBP1 suppresses let-7 miRNA maturation by binding precursors.

    Evidence RNA pulldowns, RIP, mutagenesis of intronic silencers, splicing reporter assays, telomerase activity (TRAP), miRNA precursor quantification across multiple systems

    PMID:30568224 PMID:30778047 PMID:31208978 PMID:31391218

    Open questions at the time
    • NOVA1–PTBP1 interaction interface not structurally resolved
    • Whether CD33 SNP effect on PTBP1 binding is sufficient for disease risk alone is unclear
    • Mechanism by which PTBP1 blocks Drosha/Dicer processing of let-7 precursors not determined
  9. 2019 High

    Conditional knockout studies established PTBP1 as essential for B-cell activation and humoral immunity, and showed that PTBP1 (hnRNP I) destabilizes the Notch intracellular domain to inhibit Notch signaling in intestinal homeostasis, extending its roles to immune and developmental signaling pathways.

    Evidence B-cell-specific Ptbp1 KO mouse with immunization and antibody titer measurement; zebrafish brom bones mutant with co-IP of PTBP1–NICD and Notch target analysis

    PMID:19197356 PMID:30476084

    Open questions at the time
    • Splicing targets responsible for B-cell activation defect not identified
    • PTBP1–NICD interaction mechanism (RNA-dependent vs. protein–protein) not fully clarified
  10. 2020 Medium

    lncRNAs emerged as key PTBP1 recruiters: LUCAT1 scaffolds PTBP1 onto DNA damage response pre-mRNAs altering splicing in CRC, and SINEUP lncRNAs require PTBP1 for cytoplasmic translational complex assembly, expanding PTBP1's function to lncRNA-guided post-transcriptional regulation.

    Evidence RNA pulldown, RIP, RNA-seq of alternative splicing, in vivo ASO experiments (LUCAT1); subcellular fractionation and translational complex assembly assays (SINEUP)

    PMID:31964396 PMID:33130894

    Open questions at the time
    • Whether PTBP1 binding to LUCAT1 is direct or mediated by bridging factors not resolved
    • SINEUP-PTBP1 mechanism not tested in vivo
  11. 2022 High

    PTBP1's physiological role in vascular biology was established: endothelial-specific Ptbp1 deletion reversed disturbed-flow-induced splicing changes, blocked NF-κB priming, and reduced atherosclerosis in vivo, partly through altered Ripk1 splicing.

    Evidence CRISPR KO screen, endothelial-specific conditional KO mouse, LDF model, NF-κB nuclear translocation assay, Ripk1 splicing analysis

    PMID:35858420

    Open questions at the time
    • Full spectrum of PTBP1 splicing targets mediating NF-κB priming not catalogued
    • How mechanical flow signals regulate PTBP1 activity is unknown
  12. 2022 Medium

    The lncRNA MALAT1 was shown to stabilize the PTBP1–PSF interaction, forming a tripartite regulatory module that controls a network of alternative splicing events, revealing how lncRNAs organize RBP complexes.

    Evidence RIP, co-IP of PTBP1–PSF ± MALAT1, alternative splicing RNA-seq, functional knockdown

    PMID:36563164

    Open questions at the time
    • Structural basis of MALAT1-mediated PTBP1–PSF stabilization not determined
    • Whether other lncRNAs similarly organize PTBP1 complexes is untested
  13. 2022 Medium

    PSMD14 was identified as a deubiquitinase that stabilizes PTBP1 by removing ubiquitin chains, linking proteasome-associated DUB activity to PTBP1 protein homeostasis in gastric cancer.

    Evidence Co-IP, ubiquitination assay, PSMD14 knockdown, in vitro and in vivo functional assays

    PMID:35405117

    Open questions at the time
    • Ubiquitin chain type removed by PSMD14 not specified
    • E3 ligase responsible for PTBP1 ubiquitination in this context not identified
  14. 2023 High

    A fundamental mechanistic principle was established: PTBP1-associated RNA loops within introns promote exon inclusion, while exon-spanning loops repress it, providing genome-wide predictive rules for PTBP1 splicing regulation.

    Evidence CRIC-seq (capture RIC-seq) for in situ PTBP1-associated RNA–RNA contacts in HeLa cells, combined with splicing analysis

    PMID:36958328

    Open questions at the time
    • Whether loop topology rules apply to all PTBP1 targets across cell types not tested
    • Cofactors that modulate loop formation not identified
  15. 2023 High

    Integrative structural analysis resolved how PTBP1 acts as an RNA chaperone on IRES elements: it orchestrates EMCV IRES RNA into a few compact conformations with stems exposed outward, maintaining conformational flexibility rather than imposing a single rigid fold.

    Evidence NMR, mass spectrometry, SAXS/SANS, integrative structural modeling

    PMID:37833274

    Open questions at the time
    • Whether the same chaperoning mode applies to pre-mRNA splicing substrates is unknown
    • No high-resolution structure of full-length PTBP1 on a splicing substrate
  16. 2023 High

    Endothelial-specific Ptbp1 knockout caused left ventricular noncompaction via altered Arrb1 splicing, establishing PTBP1 as essential for cardiac morphogenesis and demonstrating that PTBP1 splicing regulation in endothelial cells non-cell-autonomously controls cardiomyocyte proliferation.

    Evidence Endothelial-specific conditional Ptbp1 KO mouse, ventricular morphology analysis, Arrb1 splicing analysis

    PMID:37002228

    Open questions at the time
    • Mechanism by which Arrb1 isoform switch controls endothelial migration not fully resolved
    • Human relevance for congenital cardiac disease not established
  17. 2023 Medium

    PTBP1 was found to be present and functionally active in adult axons, with levels increasing after nerve injury; it associates with injury-response mRNAs (KPNB1, RHOA) and regulates local axonal translation, nociceptor regeneration, and sensory recovery.

    Evidence Immunofluorescence/fractionation in adult neurons, RNA-protein co-IP in axons, PTBP1 perturbation with behavioral sensory readouts, nerve injury model

    PMID:37506203

    Open questions at the time
    • Mechanism of injury-induced PTBP1 increase in axons not identified
    • Whether PTBP1 acts on splicing vs. translation in the axonal compartment not distinguished
  18. 2024 High

    Post-translational control of PTBP1 was further elaborated: KAT2B-mediated crotonylation at K266 enhances PTBP1–hnRNPA1/A2 interaction to drive PKM2 splicing and glycolysis, while USP46 stabilizes PTBP1 by removing K48-linked ubiquitin chains, linking metabolic PTMs and DUB activity to PTBP1-dependent metabolic reprogramming.

    Evidence Crotonylation proteomics, K266 mutagenesis, co-IP (PTBP1–hnRNPA1/A2), PKM isoform analysis, CRC xenograft (crotonylation); co-IP (USP46–PTBP1), K48-linked ubiquitination assay (USP46)

    PMID:38176460 PMID:39497094

    Open questions at the time
    • Decrotonylase (eraser) for K266 not identified
    • Interplay between crotonylation and lactylation on PTBP1 activity not tested
  19. 2024 High

    lncRNA DCRT was shown to bind PTBP1 in cardiomyocyte nuclei and inhibit PTBP1-mediated skipping of NDUFS2 exon 3; without DCRT, exon 3 skipping impairs mitochondrial complex I and causes cardiomyopathy, revealing an lncRNA–PTBP1 axis controlling mitochondrial function.

    Evidence RIP, DCRT KO mice (CRISPR-Cas9), NDUFS2 isoform sequencing, mitochondrial complex I activity assay, cardiac phenotyping

    PMID:38841852

    Open questions at the time
    • Whether DCRT–PTBP1 interaction is direct or requires other factors not resolved
    • Broader PTBP1 splicing targets in cardiomyocytes not catalogued
  20. 2025 High

    Metabolite-driven regulation of PTBP1 was uncovered: lactylation at K436 in glioma stem cells inhibits TRIM21-mediated ubiquitination and enhances RNA-binding capacity, stabilizing PFKFB4 mRNA and creating a feedforward glycolytic loop; SIRT1 was identified as the delactylase.

    Evidence Lactylation proteomics, K436 mutagenesis, co-IP (PTBP1–TRIM21), ubiquitination assay, SIRT1 delactylase assay, PFKFB4 mRNA stability assay in GSCs

    PMID:39570804

    Open questions at the time
    • Lactylation writer (acyltransferase) not identified
    • Whether K436 lactylation occurs in non-tumor cells not assessed
    • Interplay between K436 lactylation and K266 crotonylation not explored

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: the full structural basis of PTBP1 on pre-mRNA splicing substrates (vs. IRES), how mechanical and metabolic signals are transduced to PTBP1 PTMs, the logic governing lncRNA-mediated PTBP1 recruitment to specific targets, and the extent to which PTBP1's splicing vs. translational functions are partitioned across subcellular compartments.
  • No full-length PTBP1 structure on a pre-mRNA splicing substrate
  • Mechanotransduction pathway linking flow to PTBP1 in endothelium undefined
  • Compartment-specific interactomes (nuclear vs. axonal) not compared systematically

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 9 GO:0140098 catalytic activity, acting on RNA 9 GO:0098772 molecular function regulator activity 6 GO:0044183 protein folding chaperone 1
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 2
Pathway
R-HSA-8953854 Metabolism of RNA 11 R-HSA-1430728 Metabolism 4 R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
HNRNPA1HNRNPH1MATR3NOVA1PSFRALYRBM20TRIM21
Complex memberships
PTBP1–PSF–MALAT1 tripartite modulePTBP1–hnRNPA1/A2 splicing complex

Evidence

Reading pass · 44 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2015 PTBP1 represses Pbx1 exon 7 splicing in embryonic stem cells, suppressing expression of the neuronal Pbx1a isoform; CRISPR-Cas9 deletion of exon 7 regulatory elements induced Pbx1a expression and activated neuronal gene transcription, establishing PTBP1 as a controller of the Pbx1 transcriptional program during early neuronal development. CRISPR-Cas9 regulatory element deletion, alternative splicing analysis, transcriptional reporter assays in ESCs and NPCs eLife High 26705333
2016 PTBP1 and PTBP2 display similar RNA-binding profiles across the transcriptome (shown by knockin replacement of Ptbp2 with Ptbp1), yet exhibit distinct splicing outcomes for many neuronal exons, indicating that differential cofactor interactions rather than differential RNA binding underlie their distinct regulatory networks. Conditional knockin mouse (Ptbp1 replacing Ptbp2), transcriptome-wide eCLIP/RNA-seq comparison, Ptbp2 knockout rescue experiments Cell reports High 27926877
2015 PTBP1 and RBFOX proteins regulate the alternative splicing of micro-exons (≤51 nt) in human brain transcripts; PTBP1 binding adjacent to intronic sequences suppresses micro-exon inclusion, while RBFOX proteins promote inclusion in brain, muscle, and heart. Genomic deep-sequencing analysis of micro-exon splicing coupled with splicing factor binding data (CLIP-seq datasets) Genome research Medium 25524026
2011 PTBP1 binds an intronic polypyrimidine sequence downstream of USP5 exon 15 and promotes generation of a shorter USP5 isoform 2 in glioblastoma; PTBP1 knockdown shifted splicing toward isoform 1 and inhibited GBM cell growth and migration. Affymetrix exon arrays, PTBP1 siRNA knockdown, antisense oligonucleotide splicing modulation, cell growth/migration assays Molecular carcinogenesis Medium 21976412
2016 PTBP1 promotes PKM2 isoform expression by controlling alternative splicing of the PKM gene, thereby enhancing the Warburg effect in colorectal cancer; miR-1 and miR-133b suppress this pathway by silencing PTBP1 and switching PKM isoform expression from PKM2 to PKM1. Ectopic miRNA expression, PKM isoform RT-PCR, in vitro and in vivo growth assays, ROS measurement Oncotarget Medium 26980745
2016 PTBP1 regulates alternative splicing of cortactin exon 11; knockdown of PTBP1 causes exclusion of exon 11 and reduces cortactin isoform-a expression, which decreases colorectal cancer cell migration and invasion; overexpression of cortactin isoform-a rescues the PTBP1-knockdown motility phenotype. siRNA knockdown, RT-PCR isoform analysis, cell migration/invasion assays, in vivo shRNA tumor model, rescue overexpression Oncotarget Medium 28404950
2016 PTBP1 binds MCL1 mRNA and destabilizes it; PTBP1 knockdown increases MCL1 mRNA stability and cytoplasmic accumulation, elevating MCL1 protein and protecting cancer cells from antitubulin-induced apoptosis in an MCL1-dependent manner. RNA immunoprecipitation, mRNA stability assays, siRNA knockdown, MCL1-dependent apoptosis rescue experiments Cell death and differentiation Medium 27367564
2018 PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 mRNA, facilitating miR-101-induced apoptosis and clonogenic inhibition; PTBP1 coordinates with miRISC on a shared target mRNA. RNA immunoprecipitation of AGO2, functional co-knockdown/overexpression assays, clonogenic survival assays Cell death & disease Medium 29748555
2018 PTBP1 enhances CCND3 mRNA translation by binding its 5'-UTR, promoting cell cycle progression and tumor growth in hepatocellular carcinoma; miR-194 represses PTBP1 by binding its 3'-UTR, reducing CCND3 levels. RNA immunoprecipitation, luciferase 5'-UTR reporter assay, polysome fractionation, miRNA target validation The Journal of pathology Medium 31301177
2018 NOVA1 directs PTBP1 to hTERT pre-mRNA in a NOVA1-dependent fashion (shown by RNA pulldown); PTBP1 promotes full-length hTERT splicing and telomerase activity, while its knockdown reduces telomerase activity and leads to progressive telomere shortening. RNA pulldown, PTBP1 stable knockdown, telomerase activity assay (TRAP), telomere length measurement Oncogene Medium 30568224
2019 PTBP1 binds to polypyrimidine sequences on both upstream intron 11 and downstream intron 11a of the Mena pre-mRNA (confirmed by RNA pulldown and mutation analysis), promoting Mena exon 11a skipping; this skipping is required for PTBP1-driven lung cancer cell migration and invasion, and is reversed by MenaΔ11a overexpression. RNA pulldown with mutational analysis of binding sites, shRNA KD, overexpression rescue, migration/invasion assays Biochimica et biophysica acta. Gene regulatory mechanisms Medium 31075540
2019 PTBP1 binds SLC31A1 mRNA via RIP-confirmed interaction and reduces SLC31A1 mRNA stability, thereby decreasing copper transporter expression; PTBP1 knockdown upregulates SLC31A1 and enhances cisplatin sensitivity in chemoresistant osteosarcoma cells. RNA immunoprecipitation, dual-luciferase reporter assay, RNA stability (actinomycin D chase), siRNA knockdown, xenograft model Journal of cellular and molecular medicine Medium 32207235
2019 PTBP1 binds the precursors of let-7a-1 and let-7d miRNAs (shown by RNA pulldown and RIP) and suppresses their maturation; lncRNA H19 reduces PTBP1 levels, thereby relieving this suppression and promoting let-7 biogenesis in cholestasis. RNA pulldown, RNA immunoprecipitation (RIP), miRNA precursor quantification, BDL mouse model, H19 KO Cell death & disease Medium 30778047
2019 PTBP1 acts as a splicing enhancer for CD33 exon 2 inclusion; PTBP1 binds RNA sequences proximal to the intron 1-exon 2 splice junction, and this binding is altered by the Alzheimer's disease-associated SNP, providing a mechanism for SNP-dependent alternative splicing of CD33. Functional genomics splicing assays, proteomic PTBP1 identification, RNA binding site analysis by mutagenesis near SNP Molecular and cellular biology Medium 31208978
2019 hnRNP I (PTBP1 ortholog in zebrafish) destabilizes the Notch intracellular domain (NICD) and inhibits Notch signaling; this function requires the C-terminal RAM domain of NICD and is conserved from zebrafish to human, regulating intestinal epithelial homeostasis. Zebrafish mutant (brom bones), overexpression in zebrafish and human cells, co-IP/biochemistry with NICD, Notch target gene analysis PLoS genetics Medium 19197356
2019 TCF3 mutually exclusive alternative splicing is regulated by PTBP1 binding to conserved intronic splicing silencers (ISSs) between the two mutually exclusive exons; PTBP1 and hnRNPH1 act cooperatively through position-dependent interactions at ISSs and ESSs to control TCF3 exon 18a/18b selection. ISS mutagenesis, PTBP1 and hnRNPH1 co-knockdown/overexpression, splicing reporter assays RNA (New York, N.Y.) Medium 31391218
1997 A 60-amino-acid sequence at the amino terminus of hnRNP I/PTBP1 (designated NLD-I) is both necessary and sufficient for nuclear localization, representing a novel bipartite nuclear localization signal distinct from canonical NLS sequences in other hnRNP proteins. CAT reporter fusion constructs, transient transfection localization assay Experimental cell research Medium 9281380
2020 LUCAT1 lncRNA interacts with PTBP1 in colorectal cancer cells and facilitates association of PTBP1 with DNA damage-related gene pre-mRNAs, altering their alternative splicing and contributing to chemotherapy resistance; PTBP1 overexpression rescues LUCAT1 knockdown phenotypes. RNA pulldown, RNA immunoprecipitation, RNA-seq of alternative splicing, rescue overexpression, in vivo ASO experiments Molecular cancer Medium 31964396
2020 SINEUP lncRNAs require PTBP1 and HNRNPK for their sub-cellular distribution and for assembly of translational initiation complexes; these RBPs promote co-localization of SINEUP RNA with target mRNAs in the cytoplasm and enhance target mRNA translation. Co-transfection, subcellular fractionation, translational initiation complex assembly assays, RBP knockdown Nucleic acids research Medium 33130894
2021 SON acts as a master regulator of PTBP1-mediated oncogenic splicing in glioblastoma: SON knockdown causes failure of intron removal from the PTBP1 transcript, leading to PTBP1 downregulation; SON also forms a complex with hnRNP A2B1 to suppress RBFOX2-mediated neuronal splicing, including skipping of the PTBP2 neuronal exon. SON knockdown (siRNA/shRNA), PTBP1/PTBP2 mRNA/protein analysis, hnRNP A2B1 co-IP, GBM xenograft model Nature communications Medium 34548489
2022 MALAT1 lncRNA stabilizes the interaction between PTBP1 and PSF, forming a functional tripartite module that regulates a network of alternative splicing events; MALAT1 also stabilizes PSF interactions with several other hnRNP proteins. RNA immunoprecipitation, co-IP (PTBP1-PSF interaction with/without MALAT1), alternative splicing RNA-seq, functional knockdown Science advances Medium 36563164
2023 PTBP1-associated RNA loops within individual introns preferentially promote cassette exon splicing by accelerating asymmetric intron removal, while loops spanning across cassette exons repress splicing; these positional rules predict PTBP1-regulated splicing outcomes genome-wide. Capture RIC-seq (CRIC-seq) method for PTBP1-associated in situ proximal RNA-RNA contacts in HeLa cells, combined with splicing analysis Molecular cell High 36958328
2023 Integrative structural analysis (NMR, mass spectrometry, small-angle scattering) determined the solution structure of PTBP1 bound to an RNA fragment from the EMCV IRES; PTBP1 acts as an RNA chaperone orchestrating the IRES RNA into a few distinct compact conformations with RNA stems exposed outward, maintaining conformational flexibility. Magnetic resonance (NMR), mass spectrometry, small-angle X-ray/neutron scattering (SAXS/SANS), integrative structural modeling Nature communications High 37833274
2018 Mass spectrometry identified distinct phosphorylation and acetylation modifications on PTBP1 versus PTBP2; PTBP1 has unique phosphorylation sites in its N-terminal, linker 1, and linker 2 regions, and distinct acetylation sites in RRM2 and RRM3, suggesting PTMs contribute to their differential splicing activities. Mass spectrometry of PTMs on proteins from in vitro splicing reactions Biochemistry Medium 29851470
2022 PTBP1 interacts with distinct protein partners compared to PTBP2 under in vitro splicing conditions; PTBP1 uniquely associates with many mRNA processing and splicing regulatory proteins, while both paralogs interact with chromatin remodeling factors; potential PTM writers/erasers differ between the paralogs. In vitro splicing conditions, co-immunoprecipitation, mass spectrometry-based interactome PloS one Medium 35113929
2019 PTBP1 mRNA isoforms differ in their 5'-UTR and 3'-UTR sequences; eIF3 binds each isoform differently in a cell cycle-dependent manner and binding correlates with repression of PTBP1 protein during S phase, demonstrating translational regulation of PTBP1 expression during the cell cycle. PTBP1 mRNA isoform mapping in HEK293T cells, eIF3 RIP, cell cycle synchronization, western blot RNA (New York, N.Y.) Medium 31263002
2022 PTBP1 is required for endothelial NF-κB priming under low and disturbed blood flow (LDF); PTBP1 deletion reverses LDF-induced RNA splicing patterns, inhibits NF-κB nuclear translocation, and reduces ICAM1/VCAM1 induction by TNF-α; in vivo endothelial-specific Ptbp1 deletion reduced ICAM1 expression, myeloid infiltration, and atherosclerotic plaque in LDF regions; a mechanism involves altered splicing of Ripk1 (cassette exon inclusion reducing Ripk1 protein). CRISPR Cas9 KO screen, endothelial-specific conditional KO mouse, LDF model, NF-κB nuclear translocation assay, ICAM1/VCAM1 induction, Ripk1 splicing analysis Proceedings of the National Academy of Sciences of the United States of America High 35858420
2023 Endothelial-specific knockout of Ptbp1 in mice causes left ventricular noncompaction by reducing endothelial cell migration, disrupting cardiomyocyte proliferation; mechanistically, Ptbp1 deletion alters alternative splicing of β-arrestin-1 (Arrb1), which affects endothelial cell migration. Endothelial-specific conditional Ptbp1 knockout mouse, ventricular morphology analysis, endothelial cell migration assay, Arrb1 splicing analysis Nature communications High 37002228
2019 B-cell-specific Ptbp1 deletion impairs BCR-mediated B-cell activation and plasmablast generation, resulting in defective antibody production against T-cell-independent and T-cell-dependent antigens, demonstrating Ptbp1 is essential for the humoral immune response. B-cell-specific conditional Ptbp1 knockout mouse, NP-Ficoll and NP-CGG immunization, antibody titer measurement, plasmablast FACS International immunology High 30476084
2021 In dendritic cells, Ptbp1 deletion increases MHC II expression and perturbs T-cell homeostasis without affecting DC development; PTBP1 regulates alternative splicing of Pkm and a subset of interferon-response genes in DCs, and its deletion enhances antitumor immunity. DC-specific Ptbp1 conditional KO mouse, MHC II FACS, T-cell homeostasis analysis, alternative splicing RNA-seq, tumor challenge model Immunology Medium 33421118
2023 PTBP1 is expressed in adult sensory and motor neurons (not only embryonic neurons); axonal PTBP1 levels increase markedly after peripheral nerve injury, where it associates with injury-response mRNAs (including KPNB1/importin β1 and RHOA); perturbation of PTBP1 affects local axonal translation, nociceptor regeneration, and thermal/mechanical sensation. Immunofluorescence/fractionation for subcellular localization, RNA-protein co-IP in axons, PTBP1 perturbation with sensory behavioral readouts, nerve injury model Science advances Medium 37506203
2025 PTBP1 is lactylated at K436 in glioma stem cells; SIRT1 acts as the delactylase. K436 lactylation inhibits PTBP1 proteasomal degradation by attenuating interaction with the E3 ligase TRIM21, and enhances PTBP1 RNA-binding capacity to stabilize PFKFB4 mRNA, driving glycolysis in a feedforward loop. Lactylation proteomics, K436 site-directed mutagenesis, co-IP (PTBP1-TRIM21), ubiquitination assay, SIRT1 delactylase assay, PFKFB4 mRNA stability assay, GSC functional assays Cancer research High 39570804
2024 PTBP1 is crotonylated at K266 by KAT2B in colorectal cancer; K266 crotonylation enhances PTBP1 interaction with hnRNPA1/A2, promoting PKM2 isoform expression over PKM1 through alternative splicing, thereby increasing glycolysis and CRC progression. Crotonylation proteomics, K266 site mutagenesis, co-IP (PTBP1-hnRNPA1/2), PKM isoform RT-PCR, glycolysis assays, KAT2B identification, CRC xenograft model Journal of translational medicine High 39497094
2022 The deubiquitinating enzyme PSMD14 interacts with PTBP1 (confirmed by co-IP), promotes PTBP1 deubiquitination, and stabilizes PTBP1 protein; PSMD14 knockdown reduces PTBP1 protein levels and inhibits gastric cancer cell proliferation and invasion. Co-immunoprecipitation, ubiquitination assay, PSMD14 and PTBP1 co-localization, siRNA knockdown, in vitro and in vivo functional assays Experimental cell research Medium 35405117
2024 PTBP1 interacts with RALY (hnRNP associated with lethal yellow protein homolog) and together they regulate exon 5 splicing of DNMT3B, shifting from the DNMT3B-S to DNMT3B-L isoform; DNMT3B-L promotes promoter methylation of DUSP2, inhibiting its expression and enhancing radioresistance in prostate cancer. Co-IP (PTBP1-RALY), splicing minigene assays, DNMT3B isoform RT-PCR, DUSP2 promoter methylation (bisulfite sequencing/ChIP), KD/OE functional assays, irradiation experiments in vitro and in vivo Advanced science Medium 39287090
2024 USP46 deubiquitinates PTBP1 and stabilizes it by inhibiting K48-linked ubiquitin-mediated degradation; stabilized PTBP1 increases the PKM2/PKM1 ratio, promoting glycolysis and tamoxifen resistance in breast cancer cells. Co-IP (USP46-PTBP1), K48-linked ubiquitination assay, PKM isoform ratio analysis, glycolysis assay, siRNA KD functional assays Biochimica et biophysica acta. Molecular basis of disease Medium 38176460
2014 PTBP1 represses inclusion of ABLIM1 exon 11 in skeletal muscle; in a cellular splicing assay, PTBP1 acts together with CELF proteins and MBNL proteins to regulate this exon, and ABLIM1 exon 11 is aberrantly excluded in DM1 patients consistent with PTBP1 activity. Cellular splicing reporter assay, patient tissue RT-PCR, transfection overexpression Genes to cells Medium 25403273
2023 CTC-490G23.2 lncRNA acts as a scaffold to increase PTBP1 binding to CD44 pre-mRNA, causing a splicing switch from CD44s to CD44v(8-10); CD44v(8-10) binds vimentin and increases its protein stability, promoting cancer metastasis. RNA pulldown, RIP, CD44 isoform RT-PCR, vimentin co-IP with CD44 isoforms, invasion/metastasis assays in vitro and in vivo Oncogene Medium 36792757
2024 lncRNA DCRT directly binds PTBP1 in the nucleus of cardiomyocytes (shown by RIP and chromatin coIP) and inhibits PTBP1-mediated skipping of NDUFS2 exon 3; exon 3 skipping impairs mitochondrial complex I activity and disrupts PRDX5 antioxidant function, causing cardiomyopathy. RNA immunoprecipitation, DCRT KO mice (CRISPR-Cas9), NDUFS2 isoform sequencing, mitochondrial complex I activity assay, cardiac function phenotyping Circulation High 38841852
2020 PTBP1 participates with RBM20 in regulating alternative splicing of FHOD3 exons in cardiac tissue; co-expression of RBM20 and PTBP1 promotes alternative shift from exon inclusion to exclusion, affecting actin filament organization by FHOD3 isoforms. RT-PCR splicing analysis, PTBP1 and RBM20 overexpression in cells, FHOD3 isoform quantification in human tissues The international journal of biochemistry & cell biology Medium 30468920
2021 MALAT1 lncRNA is m6A-methylated by METTL3, promoting MALAT1 upregulation; MALAT1 interacts with PTBP1 to promote degradation of USP8 mRNA, reducing USP8 expression; reduced USP8 promotes TAK1 ubiquitination and protein instability, activating pyroptosis and M1 macrophage polarization to aggravate liver fibrosis. m6A MeRIP, RNA pulldown, RIP, RNA stability assay, co-IP (USP8-TAK1 ubiquitination), gain/loss-of-function, liver fibrosis mouse model Cell death discovery Medium 34839365
2005 Xenopus hnRNP I (PTBP1 ortholog) interacts with 40LoVe at the VM1 motif of the Vg1 localization element, forming a localization RNP complex; 40LoVe also interacts with Vg1RBP/Vera at the E2 motif; in vivo titration indicates VM1- and E2-binding proteins have distinct roles in Vg1LE-dependent mRNA localization. Co-immunoprecipitation, RNA localization element binding competition assays in Xenopus oocytes RNA (New York, N.Y.) Medium 16373488
2020 Charme lncRNA nuclear isoform (pCharme) interacts with PTBP1 and MATR3 through sequences within its alternatively spliced intron-1; CRISPR-Cas9 deletion of intron-1 releases pCharme from chromatin and causes cardiac defects similar to full-length Charme knockout, demonstrating PTBP1 is required for pCharme chromatin anchoring during myogenesis. RNA antisense purification (RAP) with mass spectrometry, CRISPR-Cas9 intron-1 deletion, chromatin fractionation, cardiac phenotyping in mice Cell reports Medium 33357424
2023 PTBP1 directly interacts with c-Myc protein and stabilizes its protein levels by preventing proteasomal degradation; this is mediated by upregulation of USP28 and limitation of FBW7-mediated c-Myc ubiquitination; PTBP1 depletion-induced tumor regression is rescued by exogenous c-Myc expression. Co-immunoprecipitation (PTBP1-c-Myc), ubiquitination assay, USP28/FBW7 expression analysis, rescue overexpression, in vivo xenograft British journal of cancer Medium 36635500

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 RBFOX and PTBP1 proteins regulate the alternative splicing of micro-exons in human brain transcripts. Genome research 150 25524026
2021 Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer. Theranostics 145 34158864
2020 Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response. Molecular cancer 132 31964396
2021 The METTL3/MALAT1/PTBP1/USP8/TAK1 axis promotes pyroptosis and M1 polarization of macrophages and contributes to liver fibrosis. Cell death discovery 124 34839365
2015 The splicing regulator PTBP1 controls the activity of the transcription factor Pbx1 during neuronal differentiation. eLife 124 26705333
2020 Roles of PTBP1 in alternative splicing, glycolysis, and oncogensis. Journal of Zhejiang University. Science. B 117 32115910
2016 PTBP1 and PTBP2 Serve Both Specific and Redundant Functions in Neuronal Pre-mRNA Splicing. Cell reports 101 27926877
2015 Network-based metaanalysis identifies HNF4A and PTBP1 as longitudinally dynamic biomarkers for Parkinson's disease. Proceedings of the National Academy of Sciences of the United States of America 81 25646437
2018 PTBP1 promotes the growth of breast cancer cells through the PTEN/Akt pathway and autophagy. Journal of cellular physiology 77 29856478
2011 PTBP1-dependent regulation of USP5 alternative RNA splicing plays a role in glioblastoma tumorigenesis. Molecular carcinogenesis 76 21976412
2008 Structure of the intracellular domain of the amyloid precursor protein in complex with Fe65-PTB2. EMBO reports 70 18833287
2016 PTBP1-associated microRNA-1 and -133b suppress the Warburg effect in colorectal tumors. Oncotarget 68 26980745
2023 N4-acetylcytidine modification of lncRNA CTC-490G23.2 promotes cancer metastasis through interacting with PTBP1 to increase CD44 alternative splicing. Oncogene 65 36792757
2017 MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget 62 28380435
2023 CircRNA MBOAT2 promotes intrahepatic cholangiocarcinoma progression and lipid metabolism reprogramming by stabilizing PTBP1 to facilitate FASN mRNA cytoplasmic export. Cell death & disease 57 36635270
2017 High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 56 28404950
2020 PTBP1 modulates osteosarcoma chemoresistance to cisplatin by regulating the expression of the copper transporter SLC31A1. Journal of cellular and molecular medicine 55 32207235
2022 Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina. Cell reports 54 35705053
2025 PTBP1 Lactylation Promotes Glioma Stem Cell Maintenance through PFKFB4-Driven Glycolysis. Cancer research 53 39570804
2019 MiR-326 Inhibits Inflammation and Promotes Autophagy in Silica-Induced Pulmonary Fibrosis through Targeting TNFSF14 and PTBP1. Chemical research in toxicology 52 31642316
2023 An in vitro-transcribed circular RNA targets the mitochondrial inner membrane cardiolipin to ablate EIF4G2+/PTBP1+ pan-adenocarcinoma. Nature cancer 49 37845485
2022 LncRNA FIRRE functions as a tumor promoter by interaction with PTBP1 to stabilize BECN1 mRNA and facilitate autophagy. Cell death & disease 48 35110535
2022 MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF. Science advances 48 36563164
2020 SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies. Nucleic acids research 46 33130894
2023 LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement. Journal of experimental & clinical cancer research : CR 45 37740243
2018 NOVA1 directs PTBP1 to hTERT pre-mRNA and promotes telomerase activity in cancer cells. Oncogene 43 30568224
2016 PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell death and differentiation 43 27367564
2019 H19 potentiates let-7 family expression through reducing PTBP1 binding to their precursors in cholestasis. Cell death & disease 40 30778047
2019 A miR-194/PTBP1/CCND3 axis regulates tumor growth in human hepatocellular carcinoma. The Journal of pathology 40 31301177
2019 Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis. Cell death & disease 39 31862871
2022 Reduced CircSMOC1 Level Promotes Metabolic Reprogramming via PTBP1 (Polypyrimidine Tract-Binding Protein) and miR-329-3p in Pulmonary Arterial Hypertension Rats. Hypertension (Dallas, Tex. : 1979) 37 35997022
2022 Critical examination of Ptbp1-mediated glia-to-neuron conversion in the mouse retina. Cell reports 36 35705044
2021 SON drives oncogenic RNA splicing in glioblastoma by regulating PTBP1/PTBP2 switching and RBFOX2 activity. Nature communications 36 34548489
2019 PTBP1 mRNA isoforms and regulation of their translation. RNA (New York, N.Y.) 36 31263002
2022 METTL3-modified lncRNA-SNHG8 binds to PTBP1 to regulate ALAS2 expression to increase oxidative stress and promote myocardial infarction. Molecular and cellular biochemistry 35 36282350
2023 Capture RIC-seq reveals positional rules of PTBP1-associated RNA loops in splicing regulation. Molecular cell 33 36958328
2022 N6-Methyladenosine-modified lncRNA LINREP promotes Glioblastoma progression by recruiting the PTBP1/HuR complex. Cell death and differentiation 33 35871232
2022 LncRNA SFTA1P promotes cervical cancer progression by interaction with PTBP1 to facilitate TPM4 mRNA degradation. Cell death & disease 33 36344495
2018 miR-124 promotes neural differentiation in mouse bulge stem cells by repressing Ptbp1 and Sox9. Journal of cellular physiology 33 30417370
2021 MicroRNA‑133b alleviates doxorubicin‑induced cardiomyocyte apoptosis and cardiac fibrosis by targeting PTBP1 and TAGLN2. International journal of molecular medicine 32 33982775
2020 The Emerging Role of the RBM20 and PTBP1 Ribonucleoproteins in Heart Development and Cardiovascular Diseases. Genes 32 32276354
2019 SRSF1 and PTBP1 Are trans-Acting Factors That Suppress the Formation of a CD33 Splicing Isoform Linked to Alzheimer's Disease Risk. Molecular and cellular biology 32 31208978
2019 Long noncoding RNA HCG22 suppresses proliferation and metastasis of bladder cancer cells by regulation of PTBP1. Journal of cellular physiology 32 31304601
2018 PTBP1 promotes tumorigenesis by regulating apoptosis and cell cycle in colon cancer. Bulletin du cancer 32 30309622
2023 Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B. Cell death & disease 31 36792603
2022 PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor. Theranostics 31 35664063
2022 Blocking lncRNA-SNHG16 sensitizes gastric cancer cells to 5-Fu through targeting the miR-506-3p-PTBP1-mediated glucose metabolism. Cancer & metabolism 30 36447254
2020 Intronic Determinants Coordinate Charme lncRNA Nuclear Activity through the Interaction with MATR3 and PTBP1. Cell reports 30 33357424
2019 PTB-AS, a Novel Natural Antisense Transcript, Promotes Glioma Progression by Improving PTBP1 mRNA Stability with SND1. Molecular therapy : the journal of the American Society of Gene Therapy 30 31253583
2019 PTBP1 enhances exon11a skipping in Mena pre-mRNA to promote migration and invasion in lung carcinoma cells. Biochimica et biophysica acta. Gene regulatory mechanisms 29 31075540
2009 hnRNP I inhibits Notch signaling and regulates intestinal epithelial homeostasis in the zebrafish. PLoS genetics 28 19197356
2005 40LoVe interacts with Vg1RBP/Vera and hnRNP I in binding the Vg1-localization element. RNA (New York, N.Y.) 28 16373488
2021 lncRNA SNHG6 promotes hepatocellular carcinoma progression by interacting with HNRNPL/PTBP1 to facilitate SETD7/LZTFL1 mRNA destabilization. Cancer letters 27 34252487
2021 Alternative microexon splicing by RBFOX2 and PTBP1 is associated with metastasis in colorectal cancer. International journal of cancer 27 34346508
2021 LncRNA SNHG1 modulates adipogenic differentiation of BMSCs by promoting DNMT1 mediated Opg hypermethylation via interacting with PTBP1. Journal of cellular and molecular medicine 27 34854215
2018 Post-Translational Modifications in Polypyrimidine Tract Binding Proteins PTBP1 and PTBP2. Biochemistry 27 29851470
2017 Automated fluorescent miscroscopic image analysis of PTBP1 expression in glioma. PloS one 27 28282372
2018 PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell death & disease 26 29748555
2022 Downregulating PTBP1 Fails to Convert Astrocytes into Hippocampal Neurons and to Alleviate Symptoms in Alzheimer's Mouse Models. The Journal of neuroscience : the official journal of the Society for Neuroscience 25 35944999
2021 LncRNA HOTTIP facilitates cell proliferation, invasion, and migration in osteosarcoma by interaction with PTBP1 to promote KHSRP level. Cell cycle (Georgetown, Tex.) 25 33475442
2014 ABLIM1 splicing is abnormal in skeletal muscle of patients with DM1 and regulated by MBNL, CELF and PTBP1. Genes to cells : devoted to molecular & cellular mechanisms 25 25403273
1997 Sequence determinants for hnRNP I protein nuclear localization. Experimental cell research 25 9281380
2023 PTBP1 as a potential regulator of disease. Molecular and cellular biochemistry 24 38129625
2019 RNA-binding protein Ptbp1 is essential for BCR-mediated antibody production. International immunology 24 30476084
2024 LncRNA DCRT Protects Against Dilated Cardiomyopathy by Preventing NDUFS2 Alternative Splicing by Binding to PTBP1. Circulation 23 38841852
2022 Splice factor polypyrimidine tract-binding protein 1 (Ptbp1) primes endothelial inflammation in atherogenic disturbed flow conditions. Proceedings of the National Academy of Sciences of the United States of America 23 35858420
2024 M6A-modified lncRNA FAM83H-AS1 promotes colorectal cancer progression through PTBP1. Cancer letters 22 38964733
2021 PTBP1 is necessary for dendritic cells to regulate T-cell homeostasis and antitumour immunity. Immunology 22 33421118
2021 Transcription factor ELK1 accelerates aerobic glycolysis to enhance osteosarcoma chemoresistance through miR-134/PTBP1 signaling cascade. Aging 22 33621196
2020 LncRNA-Malat1 is Involved in Lipotoxicity-Induced ß-cell Dysfunction and the Therapeutic Effect of Exendin-4 via Ptbp1. Endocrinology 22 32324218
2023 The role of RNA splicing factor PTBP1 in neuronal development. Biochimica et biophysica acta. Molecular cell research 21 37263298
2023 Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility. Nature communications 21 37833274
2024 Artesunate alleviates sepsis-induced liver injury by regulating macrophage polarization via the lncRNA MALAT1/PTBP1/IFIH1 axis. Diagnostic microbiology and infectious disease 20 38889486
2018 RNA-binding proteins RBM20 and PTBP1 regulate the alternative splicing of FHOD3. The international journal of biochemistry & cell biology 20 30468920
2023 PTBP1 drives c-Myc-dependent gastric cancer progression and stemness. British journal of cancer 19 36635500
2023 Therapeutic Potential of PTBP1 Inhibition, If Any, Is Not Attributed to Glia-to-Neuron Conversion. Annual review of neuroscience 19 36750409
2023 P53-response circRNA_0006420 aggravates lung cancer radiotherapy resistance by promoting formation of HUR/PTBP1 complex. Journal of advanced research 19 37541584
2019 TCF3 mutually exclusive alternative splicing is controlled by long-range cooperative actions between hnRNPH1 and PTBP1. RNA (New York, N.Y.) 19 31391218
2024 PTBP1 crotonylation promotes colorectal cancer progression through alternative splicing-mediated upregulation of the PKM2 gene. Journal of translational medicine 18 39497094
2023 PTBP1 suppresses porcine epidemic diarrhea virus replication via inducing protein degradation and IFN production. The Journal of biological chemistry 17 37392846
2022 Polypyrimidine tract binding proteins PTBP1 and PTBP2 interact with distinct proteins under splicing conditions. PloS one 17 35113929
2022 Deubiquitinating enzyme PSMD14 facilitates gastric carcinogenesis through stabilizing PTBP1. Experimental cell research 17 35405117
2021 hsa_circ_0005358 suppresses cervical cancer metastasis by interacting with PTBP1 protein to destabilize CDCP1 mRNA. Molecular therapy. Nucleic acids 17 34976440
2020 Long non-coding RNA SNHG1 regulates rheumatoid synovial invasion and proliferation by interaction with PTBP1. International immunopharmacology 17 33218941
2023 lncRNA Helf promotes hepatic inflammation and fibrosis by interacting with PTBP1 to facilitate PIK3R5 mRNA stabilization. Cellular & molecular biology letters 16 37805473
2016 Identification of neural stem cell differentiation repressor complex Pnky-PTBP1. Stem cell investigation 16 27358902
2024 USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis. Biochimica et biophysica acta. Molecular basis of disease 15 38176460
2024 PTBP1 Regulates DNMT3B Alternative Splicing by Interacting With RALY to Enhance the Radioresistance of Prostate Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 15 39287090
2024 PTBP1 knockdown impairs autophagy flux and inhibits gastric cancer progression through TXNIP-mediated oxidative stress. Cellular & molecular biology letters 14 39153986
2023 PTBP1 regulates injury responses and sensory pathways in adult peripheral neurons. Science advances 14 37506203
2020 Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1. Neural plasticity 14 32774357
2014 Arabidopsis PTB1 and PTB2 proteins negatively regulate splicing of a mini-exon splicing reporter and affect alternative splicing of endogenous genes differentially. The New phytologist 14 24749484
2024 circEPB41L2 blocks the progression and metastasis in non-small cell lung cancer by promoting TRIP12-triggered PTBP1 ubiquitylation. Cell death discovery 13 38341427
2023 Endothelial deletion of PTBP1 disrupts ventricular chamber development. Nature communications 13 37002228
2023 The lncRNA MIR99AHG directs alternative splicing of SMARCA1 by PTBP1 to enable invadopodia formation in colorectal cancer cells. Science signaling 13 37725664
2022 circEXOC5 promotes acute lung injury through the PTBP1/Skp2/Runx2 axis to activate autophagy. Life science alliance 13 36302650
2018 Suppression of PTBP1 signaling is responsible for mesenchymal stem cell induced invasion of low malignancy cancer cells. Biochimica et biophysica acta. Molecular cell research 13 30327198
2018 A developmentally regulated spliced variant of PTBP1 is upregulated in type 1 diabetic hearts. Biochemical and biophysical research communications 13 30594394
2017 Fe65-PTB2 Dimerization Mimics Fe65-APP Interaction. Frontiers in molecular neuroscience 13 28553201
2024 PTBP1-mediated biogenesis of circATIC promotes progression and cisplatin resistance of bladder cancer. International journal of biological sciences 12 38993556