Affinage

COPB2

Coatomer subunit beta' · UniProt P35606

Length
906 aa
Mass
102.5 kDa
Annotated
2026-06-09
65 papers in source corpus 30 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

COPB2 (β'-COP) is a stoichiometric WD40-repeat subunit of the cytosolic coatomer complex that coats COPI vesicles mediating retrograde and intra-Golgi membrane transport (PMID:8334999, PMID:18385291). It associates peripherally with the cis-Golgi as part of a ~550 kDa coatomer assembly, and its recruitment to Golgi membranes is ARF-GTP–dependent and blocked by brefeldin A, which acts at the initial ARF–membrane step (PMID:8458872, PMID:1631136). Antibody and siRNA loss-of-function arrest ER-to-Golgi and anterograde cargo transport and collapse ERGIC/Golgi/TGN/endosome markers into globular compartments, establishing COPB2's role across the early secretory pathway (PMID:8334707, PMID:18385291). Cargo selectivity is conferred by its WD40 domain, which recognizes KTKLL-type di-lysine retrieval motifs and is required for ER recycling of cargo such as Emp47p (PMID:14699056). Through this trafficking machinery COPB2 controls the plasma-membrane surface expression and activity of multiple ion channels and transporters — TREK1, TREK2, ANO1, TTYH2, and the Na,K-ATPase α-subunit (via a Lys54 dibasic motif) — by binding their cytoplasmic determinants (PMID:20362547, PMID:20801885, PMID:27207835, PMID:37296621, PMID:30670146). COPB2 additionally serves as RACK2, a selective anchor that recruits activated PKCε to Golgi membranes via its seven WD40 repeats (PMID:9360998, PMID:41115472), and it supports ER glycoprotein homeostasis by stabilizing EDEM3-mediated mannose trimming (PMID:40736660). Homozygous loss of Copb2 is embryonic lethal in mice, and a microcephaly-associated R254C WD40 substitution causes perinatal lethality with increased brain apoptosis and reduced layer V neurons, demonstrating an essential role in embryogenesis and corticogenesis (PMID:29036432). COPB2 is also exploited by pathogens, binding HIV-1 Nef and the Orientia effector Ank9 (PMID:18725938, PMID:28103630).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 1991 High

    Established that the COPI coat is a defined cytosolic complex whose membrane association is GTP-regulated and depends on ARF, defining the recruitment logic later applied to β'-COP.

    Evidence Protein cloning, immunoEM and subcellular fractionation of β-COP; ARF reconstitution binding assays with BFA

    PMID:1631136 PMID:1840503

    Open questions at the time
    • β'-COP/COPB2 not yet distinguished as a separate subunit
    • molecular basis of ARF-dependent recruitment not resolved
  2. 1993 High

    Identified β'-COP/COPB2 as a distinct WD40-bearing stoichiometric coatomer subunit and demonstrated that the coat is required for ER-to-Golgi transport and vesicle budding in vivo and in vitro.

    Evidence Biochemical purification with sequencing, antibody microinjection transport assays, in vitro budding reconstitution with Rab1B co-IP, quantitative immunoEM

    PMID:8334707 PMID:8334999 PMID:8376457 PMID:8458872

    Open questions at the time
    • cargo-recognition specificity of COPB2 not yet defined
    • distinction between COPB2 and COPB1 functional roles unresolved
  3. 1997 Medium

    Revealed a trafficking-independent moonlighting role: COPB2 functions as RACK2, the selective Golgi anchor for activated PKCε via its WD40 repeats.

    Evidence cDNA library screen with PKCε V1 bait, colocalization in cardiomyocytes, Golgi membrane binding assay

    PMID:9360998

    Open questions at the time
    • direct in vitro PKCε–COPB2 binding not fully reconstituted
    • physiological consequence of PKCε anchoring not established
  4. 2003 High

    Defined the structural basis of COPI cargo selection by showing the β'-COP WD40 domain reads KTKLL-type di-lysine retrieval motifs for ER recycling.

    Evidence Yeast genetics with sec27 point mutants and domain deletions, two-hybrid, Emp47p turnover assay

    PMID:14699056

    Open questions at the time
    • no high-resolution structure of the human COPB2 WD40–cargo complex in this corpus
    • full repertoire of recognized motifs incomplete
  5. 2010 High

    Connected COPB2 cargo binding to plasma-membrane surface regulation, showing it controls ion channel and transporter trafficking via specific cytoplasmic determinants.

    Evidence Yeast two-hybrid, co-IP, GST pulldown, surface biotinylation, patch-clamp, Lys54 mutagenesis and ER-retention assay for Na,K-ATPase

    PMID:20362547 PMID:20801885

    Open questions at the time
    • whether channel regulation is canonical COPI coatomer activity or subunit-specific is unresolved
    • in vivo physiological relevance of channel surface control not established
  6. 2017 High

    Demonstrated COPB2 is essential for early embryogenesis and corticogenesis, linking a WD40 microcephaly mutation to neurodevelopmental phenotypes.

    Evidence CRISPR allelic series in mice, CTIP2 immunostaining, TUNEL, neurosphere growth assays

    PMID:29036432

    Open questions at the time
    • cellular mechanism linking trafficking defect to neuronal apoptosis not defined
    • human patient genetics not addressed in this entry
  7. 2024 Medium

    Extended COPB2 function to ER glycoprotein homeostasis and EV cargo sorting, showing it stabilizes EDEM3 mannose trimming and routes PPARγ into extracellular vesicles.

    Evidence Co-IP, glycoproteomics, ER stress assays, lentiviral knockout, EV proteomics, molecular dynamics

    PMID:39601826 PMID:40736660

    Open questions at the time
    • mechanism of how a coatomer subunit directs EV sorting unclear
    • single-lab findings in specific cancer/trophoblast contexts

Open questions

Synthesis pass · forward-looking unresolved questions
  • How COPB2's canonical COPI coat function is mechanistically partitioned from its many subunit-specific roles (RACK2 anchoring, individual channel surface control, EV sorting) remains unresolved.
  • no unifying model distinguishing coatomer-dependent vs independent activities
  • structural basis for non-cargo partner binding undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0005198 structural molecule activity 2 GO:0038024 cargo receptor activity 2
Localization
GO:0005794 Golgi apparatus 3 GO:0005783 endoplasmic reticulum 2 GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-9609507 Protein localization 2
Partners
ANO1ARF1EDEM3PKCERAB1BTREK1TREK2TTYH2
Complex memberships
coatomer (COPI)

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 β-COP (COPB2 paralog note: this paper describes β-COP/COPB1, but the 1993 paper PMID:8334999 identifies β'-COP/COPB2 as a distinct novel subunit) is a peripheral 110 kDa Golgi membrane protein present in a cytosolic ~550 kDa complex (~13-14S) and localizes to non-clathrin-coated vesicles and cisternae of the Golgi complex; GTPγS treatment causes accumulation of β-COP-positive coated vesicles in Golgi fractions. Protein cloning/sequencing, immunofluorescence, immunoelectron microscopy, subcellular fractionation Cell High 1840503
1993 β'-COP (COPB2) is a novel stoichiometric subunit of the coatomer complex, present in both cytosolic coatomer and non-clathrin-coated vesicles, and shows homology to β-subunits of trimeric G proteins (WD40 repeats). Biochemical purification of coatomer, SDS-PAGE on urea-containing gels, cross-reactivity with anti-peptide antibody, N-terminal sequence analysis The EMBO journal High 8334999
1993 β-COP is essential for ER-to-Golgi biosynthetic membrane transport in vivo; microinjection of anti-β-COP antibodies blocks transport of VSV-G glycoprotein from the ER and intermediate compartment (but not from the TGN), arresting cargo in tubular structures at the ER-Golgi interface and inhibiting endoglycosidase H resistance acquisition. Microinjection of antibodies, temperature-block transport assay, VSV-G trafficking, endoglycosidase H resistance assay, immunofluorescence Cell High 8334707
1993 β-COP is required for ER-to-cis-Golgi vesicle budding in vitro; anti-β-COP antibodies/Fab fragments prevent VSV-G exit from the ER. A high-molecular-weight β-COP-containing complex (>1,000 kDa, distinct from coatomer) promotes ER vesicle budding, and Rab1B co-precipitates with this complex and is also essential for its function. In vitro ER-to-Golgi transport assay, antibody inhibition, cytosol fractionation, co-immunoprecipitation with Rab1B The Journal of cell biology High 8376457
1993 β-COP localizes predominantly to the cis-Golgi side in exocrine pancreas (68% of Golgi-associated label on cis-side); energy depletion redistributes β-COP to spherical aggregates at the cis-Golgi; BFA treatment causes >90% of β-COP to become cytoplasmic; AlF treatment causes fragmentation of Golgi into β-COP-positive vesicle clusters. Immunogold electron microscopy, energy depletion, BFA and AlF treatment, quantitative immunocytochemistry The Journal of cell biology High 8458872
1991 ARF (ADP-ribosylation factor) is required for binding of β-COP (coatomer) to Golgi membranes; coatomer resolved from ARF does not bind Golgi membranes, but binding is reconstituted by addition of recombinant ARF. Brefeldin A blocks β-COP membrane association by interfering with the initial ARF-membrane interaction step. Reconstitution binding assay with resolved fractions, ARF N-terminal peptide inhibition, BFA treatment Proceedings of the National Academy of Sciences of the United States of America High 1631136
1991 βγ subunits of heterotrimeric G proteins inhibit the GTPγS-stimulated association of both ARF and β-COP with Golgi membranes, suggesting that trimeric G proteins regulate coat protein assembly onto Golgi membranes. Membrane binding assay with purified βγ subunits added to semi-intact cells Science Medium 1957170
1997 β'-COP (COPB2) is a selective RACK (receptor for activated C-kinase) for PKCε; it was isolated by screening with the PKCε V1 region, contains seven WD40 repeats, and activated PKCε colocalizes with β'-COP in cardiac myocytes and binds Golgi membranes in a β'-COP-dependent manner. cDNA library screening with PKCε V1 bait, co-localization by immunofluorescence, binding assay on Golgi membranes The Journal of biological chemistry Medium 9360998
1994 HIV-1 Nef physically interacts with β-COP (COPB2); identified by yeast two-hybrid screening and confirmed by in vitro binding and co-immunoprecipitation from HIV-1-infected cells. Yeast two-hybrid, in vitro binding assay, co-immunoprecipitation The Journal of biological chemistry Medium 7982906
1999 HIV-1 Nef recruits β-COP (COPB2) in endosomes via a diacidic motif to target internalized CD4 for lysosomal degradation; a sequence encompassing a critical acidic dipeptide in Nef's C-terminal loop is responsible for β-COP recruitment and routing to lysosomes. Mutagenesis of Nef diacidic motif, co-immunoprecipitation, subcellular fractionation, CD4 degradation assay Cell Medium 10199403
2001 Mutation of the diacidic (glutamate) residues in HIV-1 Nef does not significantly affect its ability to interact with β-COP, downregulate CD4 surface expression, or route cargo to the endocytic pathway — indicating these acidic residues are NOT the β-COP-binding determinant as previously proposed. Site-directed mutagenesis of Nef diacidic motif, co-immunoprecipitation, CD4 surface downregulation assay, endocytic routing assay Journal of virology Medium 11264386
2008 HIV-1 Nef contains two separable β-COP-binding sites: an RXR motif in the N-terminal α-helical domain required for maximal MHC-I degradation, and a di-acidic motif in the C-terminal loop needed for efficient CD4 degradation. Both MHC-I and CD4 are ultimately targeted for degradation via β-COP activity in the same Rab7+ vesicles. Mutagenesis of Nef RXR and di-acidic motifs, co-immunoprecipitation, MHC-I and CD4 degradation assays, Rab7 co-localization PLoS pathogens Medium 18725938
2003 The WD40 domain of β'-COP (COPB2/Sec27 in yeast) mediates cargo-selective interaction with KTKLL-type di-lysine motifs and is required for recycling of Emp47p back to the ER; the two WD40 domains of α-COP and β'-COP bind distinct but overlapping sets of di-lysine signals, and loss of both WD40 domains is lethal in yeast. Yeast genetics (point mutations and domain deletions in sec27), two-hybrid, turnover assay for Emp47p, invertase maturation assay Molecular biology of the cell High 14699056
2000 Rab2 requires PKC ι/λ to recruit β-COP to pre-Golgi intermediate (VTC) membranes; PKC ι/λ translocates to membranes in a Rab2-dependent manner, and depletion of PKC ι/λ prevents β-COP recruitment. PKC ι/λ kinase activity (but not its structural presence) is required for Rab2-mediated vesicle budding. Quantitative membrane binding assay, antibody depletion of PKC isoforms, kinase-deficient PKC mutant, pseudosubstrate peptide inhibition Traffic Medium 11208158
2006 Stomach-specific calpain nCL-2 (calpain-8a) co-localizes with β-COP at the Golgi in COS7 cells and proteolytically cleaves β-COP near its linker region in vitro and in cells upon Ca2+-ionophore stimulation, causing dissociation of β-COP from the Golgi. Yeast two-hybrid, co-localization by immunofluorescence, in vitro proteolysis assay, Ca2+-ionophore stimulation, Western blot The Journal of biological chemistry Medium 16476741
2008 siRNA-mediated depletion of β-COP (COPB2) causes co-localization of ERGIC, Golgi, TGN, and recycling endosome markers in large globular compartments, arrests anterograde trafficking of VSV-G and caveolin-1 (Cav1), and perturbs transferrin recycling; Cav1 co-precipitates with γ-COP subunit, identifying it as a COP-I cargo. siRNA knockdown, immunofluorescence, VSV-G trafficking assay, co-immunoprecipitation of Cav1 with γ-COP American journal of physiology. Cell physiology Medium 18385291
2010 β-COP interacts with the N-terminal region of TREK1 K+ channel (identified by yeast two-hybrid); co-expression of β-COP with TREK1 increases channel surface expression and activity, while β-COP shRNA reduces TREK1 surface expression. Yeast two-hybrid, co-immunoprecipitation, GST pulldown, surface biotinylation, patch-clamp electrophysiology, shRNA knockdown Biochemical and biophysical research communications Medium 20362547
2010 β-COP interacts with the Na,K-ATPase α-subunit via a dibasic motif at Lys54; in the absence of the Na,K-ATPase β-subunit, the α-subunit interacts with β-COP and is retained in the ER for degradation. Mutation of Lys54 abolishes β-COP interaction and allows α-subunit trafficking to the plasma membrane without β-subunit assembly. Novel labeling technique, co-immunoprecipitation, pulse-chase, site-directed mutagenesis of Lys54, ER retention assay The Journal of biological chemistry High 20801885
2004 β'-COP (COPB2) directly binds ADIP (afadin- and α-actinin-binding protein); ADIP co-localizes with β'-COP at the Golgi complex in MDCK and NRK cells, suggesting involvement of β'-COP in Golgi-ER vesicle trafficking through interaction with this adherens junction protein. Yeast two-hybrid, co-immunoprecipitation, co-localization by immunofluorescence Biochemical and biophysical research communications Low 15358183
2016 β-COP (COPB2) directly interacts with ANO1 (Anoctamin-1 chloride channel); co-expression of β-COP with ANO1 decreases ANO1 surface expression and channel activity, and β-COP silencing in U251 glioblastoma cells enhances endogenous ANO1 surface expression and whole-cell currents. Yeast two-hybrid, co-immunoprecipitation, GST pulldown, surface biotinylation, patch-clamp electrophysiology, β-COP siRNA Biochemical and biophysical research communications Medium 27207835
2017 The Orientia tsutsugamushi effector Ank9 binds host COPB2, which mediates Golgi-to-ER retrograde transport; COPB2 siRNA treatment destabilizes the Golgi similarly to Ank9 expression, and COPB2 reduction benefits Orientia intracellular replication. Co-immunoprecipitation, siRNA knockdown, Golgi morphology assay, intracellular replication assay Cellular microbiology Medium 28103630
2017 Homozygous loss-of-function of Copb2 is lethal at early embryogenesis in mice; compound heterozygotes (Copb2R254C/Zfn, carrying the human microcephaly-associated R254C substitution in a WD40 domain) show perinatal lethality, increased apoptosis in the brain, reduced layer V (CTIP2+) neurons, and neurosphere growth defects. CRISPR-Cas9 allelic series generation, mouse genetics, immunostaining (CTIP2), TUNEL apoptosis assay, neurosphere growth assay Human molecular genetics High 29036432
2019 β-COP (COPB2) is involved in apolipoprotein-mediated cholesterol exocytosis; β-COP knockdown reduces apoA-1-mediated cholesterol efflux in THP-1 macrophages, β-COP co-localizes with apoA-1/apoE on membrane protrusion complexes during cholesterol efflux, and apoA-1 promotes β-COP translocation to the cell membrane. lentiviral shRNA knockdown, confocal microscopy, immunogold electron microscopy, cholesterol efflux assay, Western blot, proteomics PloS one Medium 26986486
2021 COPB2 knockdown in mutant EGFR NSCLC cells alters post-translational processing of receptor tyrosine kinases (RTKs) and activates the ER stress response pathway; the small molecule EMI66 alters electrophoretic mobility and subcellular localization of COPB2 within the early secretory pathway and recapitulates RTK expression changes. siRNA knockdown, Western blot, immunofluorescence for subcellular localization, small molecule treatment (EMI66), organoid growth assay Journal of molecular biology Medium 34662547
2022 β-COP directly binds TREK1 (but not TWIK1) in the TWIK1/TREK1 heterodimeric channel complex in astrocytes; β-COP enhances surface expression of the TWIK1/TREK1 heterodimer in a TREK1-dependent manner and thereby regulates passive conductance (background K+ current) in mouse brain astrocytes. Co-immunoprecipitation, surface biotinylation, patch-clamp electrophysiology in astrocytes, heterologous expression system Cells Medium 36291187
2023 β-COP binds the C-terminus of TREK2 (but not TRAAK) and reduces TREK2 surface expression; C-terminal deletion or point mutations in TREK2 abolish β-COP binding and prevent β-COP-mediated reduction of surface expression. Co-immunoprecipitation, surface biotinylation, mutagenesis of TREK2 C-terminus Cells Medium 37296621
2019 β-COP (COPB2) directly interacts with TTYH2 anion channel (identified by yeast two-hybrid); co-expression of β-COP reduces surface expression and activity of TTYH2, and overexpression of β-COP in LoVo colon cancer cells decreases endogenous TTYH2 surface expression and activity. Yeast two-hybrid, co-immunoprecipitation, GST pulldown, surface biotinylation, whole-cell current recording, β-COP overexpression BMB reports Medium 30670146
2024 β'-COP (COPB2) directly interacts with PPARγ in trophoblasts (validated by co-immunoprecipitation and molecular dynamics simulation identifying critical binding sites); β'-COP mediates sorting of PPARγ into early endosomes and multivesicular bodies for incorporation into extracellular vesicles, and knockout of β'-COP impairs PPARγ loading into EVs. Co-immunoprecipitation, molecular dynamics simulation, lentiviral knockout and overexpression, proteomic analysis of EVs Cellular and molecular life sciences Medium 39601826
2025 β'-COP (COPB2) interacts with EDEM3 (an ERAD enzyme) in ovarian cancer cells, enhancing EDEM3 ER localization and its mannose-trimming function; COPB2 depletion impairs EDEM3 activity, causes glycan processing defects and ER stress accumulation. Co-immunoprecipitation, glycoproteomic analysis, COPB2 knockdown/overexpression, ER stress assays, xenograft in vivo model Cellular oncology Medium 40736660
2025 Inhibitory peptides derived from PKCε (KxKxx motif pentapeptides with C-terminal carboxylate, especially KIKIC) potently inhibit the PKCε–RACK2/COPB2 interaction in a proximity-based assay; alanine scanning confirmed the two Lys residues and C-terminal carboxylate as critical. KIKIC modifies PKCε translocation in cells, and RACK2 pulldown identified several proteins in a PKCε-RACK2 complex in a KIKIC-sensitive manner. Proximity-based chemiluminescent binding assay, alanine scan mutagenesis, PKCε translocation assay, RACK2 pulldown with mass spectrometry Biochimica et biophysica acta. Molecular cell research Medium 41115472
2025 Knockdown of COPB2 (along with other COPI subunits COPA, COPB1, COPG1, ARCN1, COPZ1) in Huh-7 hepatocarcinoma cells decreases uptake of HDL holoparticles and selective HDL lipid uptake by reducing cell surface SR-BI abundance and its glycosylation; COPB2 knockdown also decreases APOA1 expression and apoA-I secretion but increases cell-surface ABCA1 abundance and ABCA1-mediated cholesterol efflux. Genome-wide RNAi screen, targeted siRNA knockdown validation, flow cytometry for surface receptor abundance, cholesterol efflux assay, apoA-I secretion assay bioRxivpreprint Medium

Source papers

Stage 0 corpus · 65 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1991 Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 473 1840503
1992 ADP-ribosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein beta-COP to Golgi membranes. Proceedings of the National Academy of Sciences of the United States of America 440 1631136
1991 Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein. Science (New York, N.Y.) 346 1957170
1993 Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Cell 306 8334707
1999 Nef-induced CD4 degradation: a diacidic-based motif in Nef functions as a lysosomal targeting signal through the binding of beta-COP in endosomes. Cell 243 10199403
1997 The coatomer protein beta'-COP, a selective binding protein (RACK) for protein kinase Cepsilon. The Journal of biological chemistry 212 9360998
1993 Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas. The Journal of cell biology 185 8458872
1994 Physical interaction of the HIV-1 Nef protein with beta-COP, a component of non-clathrin-coated vesicles essential for membrane traffic. The Journal of biological chemistry 151 7982906
1993 Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro. The Journal of cell biology 132 8376457
2008 HIV-1 Nef targets MHC-I and CD4 for degradation via a final common beta-COP-dependent pathway in T cells. PLoS pathogens 128 18725938
1995 Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN. Journal of cell science 114 7593324
1993 beta'-COP, a novel subunit of coatomer. The EMBO journal 103 8334999
2003 The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Molecular biology of the cell 85 14699056
2017 Copb2 is essential for embryogenesis and hypomorphic mutations cause human microcephaly. Human molecular genetics 53 29036432
2008 Depletion of beta-COP reveals a role for COP-I in compartmentalization of secretory compartments and in biosynthetic transport of caveolin-1. American journal of physiology. Cell physiology 50 18385291
2019 COPB2 is up-regulated in breast cancer and plays a vital role in the metastasis via N-cadherin and Vimentin. Journal of cellular and molecular medicine 48 31119859
2001 Nef-induced CD4 downregulation: a diacidic sequence in human immunodeficiency virus type 1 Nef does not function as a protein sorting motif through direct binding to beta-COP. Journal of virology 48 11264386
1991 Involvement of beta-COP in membrane traffic through the Golgi complex. Trends in cell biology 48 14731804
2019 MicroRNA-4461 derived from bone marrow mesenchymal stem cell exosomes inhibits tumorigenesis by downregulating COPB2 expression in colorectal cancer. Bioscience, biotechnology, and biochemistry 47 31631786
2000 Rab2 requires PKC iota/lambda to recruit beta-COP for vesicle formation. Traffic (Copenhagen, Denmark) 42 11208158
2018 COPB2 promotes cell proliferation and tumorigenesis through up-regulating YAP1 expression in lung adenocarcinoma cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 40 29674272
2017 Orientia tsutsugamushi Ank9 is a multifunctional effector that utilizes a novel GRIP-like Golgi localization domain for Golgi-to-endoplasmic reticulum trafficking and interacts with host COPB2. Cellular microbiology 40 28103630
2021 Early prediction of COVID-19 severity using extracellular vesicle COPB2. Journal of extracellular vesicles 38 34122778
2003 Identification and localization of a beta-COP-like protein involved in the morphodynamics of the plant Golgi apparatus. Journal of experimental botany 37 12885863
2016 COPB2 Is Upregulated in Prostate Cancer and Regulates PC-3 Cell Proliferation, Cell Cycle, and Apoptosis. Archives of medical research 32 27986120
2019 Silencing of COPB2 inhibits the proliferation of gastric cancer cells and induces apoptosis via suppression of the RTK signaling pathway. International journal of oncology 29 30968146
1999 Closing in on the BPES gene on 3q23: mapping of a de Novo reciprocal translocation t(3;4)(q23;p15.2) breakpoint within a 45-kb cosmid and mapping of three candidate genes, RBP1, RBP2, and beta'-COP, distal to the breakpoint. Genomics 28 10191085
2013 RNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonis. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 27 24382395
2010 Enhancement of TREK1 channel surface expression by protein-protein interaction with beta-COP. Biochemical and biophysical research communications 27 20362547
2021 Chrysin Induced Cell Apoptosis Through H19/let-7a/COPB2 Axis in Gastric Cancer Cells and Inhibited Tumor Growth. Frontiers in oncology 25 34150620
2020 COPB2 gene silencing inhibits colorectal cancer cell proliferation and induces apoptosis via the JNK/c-Jun signaling pathway. PloS one 25 33211699
2017 COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle-related proteins. Experimental and therapeutic medicine 25 29399086
2006 Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP. The Journal of biological chemistry 24 16476741
2016 Surface expression of the Anoctamin-1 (ANO1) channel is suppressed by protein-protein interactions with β-COP. Biochemical and biophysical research communications 21 27207835
1993 Yeast coatomer contains a subunit homologous to mammalian beta'-COP. FEBS letters 20 8405452
2021 COPB2: a transport protein with multifaceted roles in cancer development and progression. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 18 34101128
2011 Biochemical and localization analyses of putative type III secretion translocator proteins CopB and CopB2 of Chlamydia trachomatis reveal significant distinctions. Infection and immunity 18 21606186
2020 MiR-216a-3p regulates the proliferation, apoptosis, migration, and invasion of lung cancer cells via targeting COPB2. Bioscience, biotechnology, and biochemistry 16 32619135
2018 Downregulation of COPB2 by RNAi inhibits growth of human cholangiocellular carcinoma cells. European review for medical and pharmacological sciences 16 29509246
2017 Coatomer subunit beta 2 (COPB2), identified by label-free quantitative proteomics, regulates cell proliferation and apoptosis in human prostate carcinoma cells. Biochemical and biophysical research communications 16 29129687
2019 High expression of COPB2 predicts adverse outcomes: A potential therapeutic target for glioma. CNS neuroscience & therapeutics 15 31710183
2010 Association with {beta}-COP regulates the trafficking of the newly synthesized Na,K-ATPase. The Journal of biological chemistry 14 20801885
2021 Chemical Genetics Screen Identifies COPB2 Tool Compounds That Alters ER Stress Response and Induces RTK Dysregulation in Lung Cancer Cells. Journal of molecular biology 10 34662547
2004 Afadin- and alpha-actinin-binding protein ADIP directly binds beta'-COP, a subunit of the coatomer complex. Biochemical and biophysical research communications 10 15358183
2000 Cloning and characterization of beta-COP from Dictyostelium discoideum. European journal of cell biology 10 10887966
2022 β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes. Cells 9 36291187
2019 Surface expression of TTYH2 is attenuated by direct interaction with β-COP. BMB reports 9 30670146
2016 β-COP as a Component of Transport Vesicles for HDL Apolipoprotein-Mediated Cholesterol Exocytosis. PloS one 9 26986486
2020 Upregulation of the Coatomer Protein Complex Subunit beta 2 (COPB2) Gene Targets microRNA-335-3p in NCI-H1975 Lung Adenocarcinoma Cells to Promote Cell Proliferation and Migration. Medical science monitor : international medical journal of experimental and clinical research 8 32004259
2022 Implication of COPB2 Expression on Cutaneous Squamous Cell Carcinoma Pathogenesis. Cancers 7 35454945
2022 Upregulation of COPB2 Promotes Prostate Cancer Proliferation and Invasion Through the MAPK/TGF-β Signaling Pathway. Frontiers in oncology 7 35600351
2021 An Integrative Pan-Cancer Analysis of the Oncogenic Role of COPB2 in Human Tumors. BioMed research international 7 34676262
2022 Lysine demethylase 5A promotes prostate adenocarcinoma progression by suppressing microRNA-330-3p expression and activating the COPB2/PI3K/AKT axis in an ETS1-dependent manner. Journal of cell communication and signaling 6 35581421
2017 Detection of COPB2 as a KRAS synthetic lethal partner through integration of functional genomics screens. Oncotarget 6 28415695
2021 Silencing the COPB2 gene decreases the proliferation, migration and invasion of human triple-negative breast cancer cells. Experimental and therapeutic medicine 5 34093748
2020 COPB2 promotes metastasis and inhibits apoptosis of lung adenocarcinoma cells through functioning as a target of miR-216a-3p. Translational cancer research 5 35117624
1999 Identification of the mouse beta'-COP Golgi component as a spermatocyte autoantigen in scleroderma and mapping of its gene Copb2 to mouse chromosome 9. Cytogenetics and cell genetics 4 10702668
2025 COPB2 promotes hepatocellular carcinoma progression through regulation of YAP1 nuclear translocation. Oncology research 3 40191726
2023 β-COP Suppresses the Surface Expression of the TREK2. Cells 3 37296621
2022 Comprehensive analysis reveals COPB2 and RYK associated with tumor stages of larynx squamous cell carcinoma. BMC cancer 3 35715770
2023 Novel insight into the phenotype of microcephaly 19 in the patient with missense COPB2 mutation. European journal of medical genetics 2 37734708
2024 β'-COP mediated loading of PPARγ into trophoblast-derived extracellular vesicles. Cellular and molecular life sciences : CMLS 1 39601826
2026 COPB2 drives gastric cancer progression via PI3K/AKT/NF-κB signaling: a multi-omics and functional study. Cell adhesion & migration 0 41618837
2025 COPB2 facilitates EDEM3-mediated mannose trimming to sustain ER homeostasis in ovarian cancer. Cellular oncology (Dordrecht, Netherlands) 0 40736660
2025 Cell-penetrant peptides as novel inhibitors of the interaction of coatomer protein COPB2/RACK2 with protein kinase Cε and cargo proteins. Biochimica et biophysica acta. Molecular cell research 0 41115472

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