Affinage

RAB1B

Ras-related protein Rab-1B · UniProt Q9H0U4

Length
201 aa
Mass
22.2 kDa
Annotated
2026-06-10
45 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB1B is a small GTPase that drives vesicular transport from the endoplasmic reticulum to the cis- and medial-Golgi, established by cell-free reconstitution and antibody-inhibition assays (PMID:1918138). It cycles between GDP- and GTP-bound states using intrinsic GTPase activity and conserved nucleotide-binding residues, where Lys21 governs GTP binding and Ala65 modulates hydrolysis (PMID:2509243); correct membrane targeting depends on this nucleotide cycle, since both inactive and constitutively active mutants fail to integrate tightly into target membranes (PMID:10493955). Membrane delivery further requires C-terminal geranylgeranylation by Rab-GGTase, which depends on the effector domain (I41, D44) (PMID:8325834) and on prior REP binding mediated by the Switch 2 helix to the GDP-bound form (PMID:9437002), with prenylated RAB1B subsequently recycled via GDI complexes (PMID:8631911). In its GTP-active state at ER exit sites and the Golgi, RAB1B acts upstream of GBF1, ARF1, and COPI coat recruitment to execute and regulate Golgi-directed transport, with GBF1 identified as a direct effector (PMID:12802079, PMID:17429068); it coordinates with COPII components Sec23/24/31 at ER exit sites (PMID:21093099), binds the tethering proteins GM130 and p115 in nucleotide-dependent fashion (PMID:11306556, PMID:25332841), and dynamically cycles between ERES, VTCs, and the Golgi (PMID:27500526). Through this trafficking activity RAB1B mediates ER-to-Golgi transport and maturation of diverse cargoes including βAPP, the LDL receptor, and von Willebrand factor, the last under transcriptional control of RUNX1 (PMID:7738040, PMID:8673720, PMID:29632235). Beyond classical secretion, RAB1B associates with ATG9A vesicles to support autophagosome formation (PMID:28522593), partners with the phosphoinositide phosphatase MTMR6 (PMID:23188820), recruits the effector GOLPH3 to promote Golgi-based secretion (PMID:26977884, PMID:32790781), forms a TRAF3 complex that bridges TRAF3 to MAVS to enhance RIG-I-driven IFN-β signaling (PMID:31375559), and directs DGAT2 from the ER to lipid droplet surfaces to drive lipid droplet growth (PMID:38809969). RAB1B is targeted by multiple GAPs that inactivate it, including the Legionella effector LepB (PMID:23288104), TBC1D20 (linked to Warburg Micro syndrome) (PMID:38809969), and TBC1D22B (PMID:40878439), and is hijacked by bacterial pathogens through covalent switch-region modifications: Legionella DrrA acts as a GEF and AMPylates Tyr77 to lock RAB1B active (PMID:20651120), AnkX phosphocholinates Ser76, and Lem3 reverses this by locally unfolding the switch II region (PMID:37076474).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1989 High

    Established that RAB1B is a genuine GTP-binding protein with intrinsic GTPase activity, defining the nucleotide-dependent switch that underlies its function.

    Evidence In vitro GTP/GDP binding and GTPase assays with purified recombinant protein and site-directed mutants (Lys21Met, Ala65Thr)

    PMID:2509243

    Open questions at the time
    • Did not link biochemical activity to a cellular process
    • GEFs and GAPs regulating the cycle not yet identified
  2. 1991 High

    Defined the core cellular role of RAB1B as required for ER-to-cis-Golgi and intra-Golgi vesicular transport.

    Evidence Cell-free reconstitution of ER-to-Golgi transport with monoclonal antibody inhibition and subcellular immunolocalization

    PMID:1918138

    Open questions at the time
    • Molecular effectors mediating transport not identified
    • Mechanism of vesicle budding vs tethering unresolved
  3. 1993 High

    Mapped the determinants of RAB1B membrane attachment, showing the effector domain is required for geranylgeranylation.

    Evidence In vitro isoprenylation assay in reticulocyte lysates with systematic effector-domain mutagenesis (I41N, D44N)

    PMID:8325834

    Open questions at the time
    • Did not resolve how prenylation enzyme is recruited
    • Role of REP not yet defined
  4. 1995 High

    Connected RAB1B trafficking activity to physiological cargo maturation, showing it is required for ER-to-Golgi transport of βAPP and the LDL receptor.

    Evidence Dominant-negative RAB1B (N121I, S22N) co-expression with cargo in 293 cells, pulse-chase, Endo-H sensitivity, and surface labeling

    PMID:7738040 PMID:8673720

    Open questions at the time
    • Cargo selectivity vs general transport block not distinguished
    • Step in the pathway where RAB1B acts not yet placed
  5. 1998 High

    Resolved the prenylation prerequisites, showing REP binds GDP-bound RAB1B via the Switch 2 helix to enable geranylgeranylation and that GDI recycling follows.

    Evidence Cell-free geranylgeranylation, metabolic labeling, nucleotide-binding assays, gel-filtration co-fractionation, and GDI co-IP with effector-domain mutants

    PMID:8631911 PMID:8836150 PMID:9437002

    Open questions at the time
    • Conflicting in vitro vs in vivo conformational preference for prenylation
    • Structural basis of REP recognition not solved here
  6. 2007 High

    Placed RAB1B upstream of the GBF1/ARF1/COPI machinery, identifying GBF1 as a direct effector and demonstrating rapid membrane cycling at the Golgi.

    Evidence Dominant mutant epistasis with ARF1/GBF1 rescue, reciprocal Co-IP, siRNA, and FRAP

    PMID:12802079 PMID:17429068

    Open questions at the time
    • Order of effector engagement relative to tethering not fully resolved
    • How GEF input is delivered to RAB1B at ERES unclear
  7. 2014 Medium

    Integrated tethering factors GM130 and p115 and the COPII coat into the RAB1B trafficking cycle, defining its budding-to-tethering coordination.

    Evidence Yeast two-hybrid and domain-mapping Co-IP for GM130/p115, COPII (Sec23/24/31) Co-IP and FRAP, and p115 epistasis rescue

    PMID:11306556 PMID:21093099 PMID:25332841 PMID:27500526

    Open questions at the time
    • Hierarchy among p115, GM130, GBF1 inputs not fully ordered
    • Direct vs indirect COPII engagement uncertain
  8. 2013 High

    Defined how Legionella locks and unlocks the RAB1B switch, revealing DrrA as a GEF and AMPylase (Tyr77) and LepB as a structurally atypical GAP.

    Evidence In vitro AMPylation with MS site mapping, GEF/GAP-accessibility assays, and crystal structure of the RAB1B:LepB complex

    PMID:20651120 PMID:23288104

    Open questions at the time
    • Host enzymes counteracting bacterial AMPylation not identified here
    • Physiological host GAP for RAB1B not yet characterized
  9. 2017 Medium

    Extended RAB1B function beyond classical secretion into autophagy and phosphoinositide regulation via ATG9A vesicles and MTMR6.

    Evidence ATG9A vesicle immunoisolation proteomics with siRNA autophagy-flux assays; nucleotide-state Co-IP with MTMR6 and omegasome assays

    PMID:23188820 PMID:28522593

    Open questions at the time
    • Whether autophagy role is separable from ER-to-Golgi transport unclear
    • Direct effector at autophagosome formation sites not defined
  10. 2019 Medium

    Revealed signaling and translational roles, showing RAB1B bridges TRAF3 to MAVS for IFN-β induction and directly binds FMDV IRES RNA to stimulate translation.

    Evidence Reciprocal Co-IP and CRISPR deletion with IFN-β reporters and viral infection; direct RNA binding, IRES translation assays, and RNA-FISH

    PMID:30655362 PMID:31375559

    Open questions at the time
    • Whether RNA binding and signaling depend on trafficking activity unresolved
    • Structural basis of TRAF3 and RNA engagement unknown
  11. 2025 Medium

    Expanded the regulatory and effector landscape, defining GOLPH3 as an effector, identifying TBC1D20 and TBC1D22B as host GAPs, and linking RAB1B to lipid droplet growth via DGAT2 redistribution.

    Evidence Nucleotide-state Co-IP for GOLPH3; FRET-based DGAT2 redistribution with TBC1D20 disease-model fibroblasts; proximity-labeling proteomics and RUSH transport assays for TBC1D22B

    PMID:26977884 PMID:32790781 PMID:38809969 PMID:40878439

    Open questions at the time
    • Disease mechanism of Warburg Micro syndrome via RAB1B not directly established here
    • How distinct GAPs partition RAB1B functions spatially unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RAB1B's many roles—ER-to-Golgi transport, autophagy, lipid droplet growth, antiviral signaling, and RNA binding—are spatially and temporally partitioned by distinct GEFs, GAPs, and effectors remains unresolved.
  • No unified model coordinating secretory vs non-secretory functions
  • Endogenous GEFs for most contexts undefined
  • Structural basis of effector selectivity across functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 4 GO:0060089 molecular transducer activity 3 GO:0003723 RNA binding 1
Localization
GO:0005794 Golgi apparatus 5 GO:0005783 endoplasmic reticulum 4 GO:0005829 cytosol 2 GO:0005811 lipid droplet 1
Pathway
R-HSA-9609507 Protein localization 4 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9612973 Autophagy 2 R-HSA-1430728 Metabolism 1 R-HSA-168256 Immune System 1
Partners
DGAT2GBF1GM130GOLPH3MTMR6P115TBC1D22BTRAF3

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 RAB1B is required for vesicular transport from the ER to the cis-Golgi compartment and between successive cis- and medial-Golgi compartments, as demonstrated by reconstitution of ER-to-Golgi transport in a cell-free assay and immunolocalization of RAB1B to both ER and Golgi compartments. Cell-free reconstitution assay of ER-to-Golgi transport; monoclonal antibody inhibition; subcellular fractionation and immunolocalization The Journal of Cell Biology High 1918138
1989 RAB1B protein binds GTP and GDP specifically and possesses intrinsic GTPase activity; the Lys21→Met mutant abolishes GTP binding, while the Ala65→Thr mutant reduces GTPase activity and retains autophosphorylation competence in the presence of GTP. In vitro biochemical assay with purified recombinant protein; site-directed mutagenesis; GTP/GDP binding and GTPase activity measurements FEBS Letters High 2509243
1993 The effector domain of RAB1B (residues I41, D44) is essential for geranylgeranylation by GGTase II; mutations I41N and D44N in the effector domain essentially abolish isoprenylation, while mutations in the N-terminal variable region, β3 strand, or Loop 7 do not reduce isoprenylation. In vitro isoprenylation assay using reticulocyte lysates; site-directed mutagenesis; deletion analysis The Journal of Biological Chemistry High 8325834
1995 RAB1B is required for ER-to-Golgi transport of beta-amyloid precursor protein (βAPP); dominant-negative RAB1B mutants (N121I, S22N) block conversion of immature Endo-H-sensitive βAPP to mature O-glycosylated form and inhibit secretion of APP-α and Aβ peptide. Co-expression of dominant-negative RAB1B mutants with βAPP in 293 cells; [35S]methionine pulse-chase; Endo-H sensitivity assay; immunoprecipitation The Journal of Biological Chemistry High 7738040
1995 Dominant-negative RAB1B (N121I) blocks ER-to-Golgi transport and maturation of the LDL receptor, preventing its conversion from the Endo-H-sensitive 120-125 kDa form to the mature 160-170 kDa form and preventing its delivery to the cell surface. Co-expression of dominant-negative RAB1B with LDL receptor in 293 cells; [35S]methionine pulse-chase; Endo-H sensitivity; sulfo-NHS-biotin cell surface labeling Journal of Receptor and Signal Transduction Research Medium 8673720
1996 Association of geranylgeranylated RAB1B with GDP-dissociation inhibitors (GDI-α and GDI-2) is required for recycling of prenylated RAB1B to the cytosol but not for initial membrane targeting; the effector-domain mutant D44N fails to form GDI complexes yet is still delivered to intracellular membranes. Co-immunoprecipitation with anti-FLAG beads; in vitro GDI binding assay; [3H]mevalonate metabolic labeling; subcellular fractionation; immunofluorescence The Journal of Biological Chemistry High 8631911
1996 RAB1B geranylgeranylation by Rab-GGTase preferentially requires the GDP-bound conformation of the substrate in cell-free assays; the GTPase-deficient Q67L mutant is poorly prenylated when GTP predominates but prenylated normally when GDP is the predominant nucleotide. Cell-free geranylgeranylation assay with defined nucleotide compositions; [3H]mevalonate metabolic labeling in transfected 293 cells; co-IP with GDI The Biochemical Journal Medium 8836150
1998 The Switch 2 domain (α2 helix, residues I73, Y78, A81) of RAB1B is essential for binding to Rab escort protein (REP); mutations in this helix prevent prenylation by preventing association of nascent RAB1B with REP, while REP binds preferentially to GDP-bound RAB1B. Cell-free geranylgeranylation assay; [3H]mevalonate metabolic labeling; [32P]orthophosphate nucleotide binding assay; gel filtration co-fractionation of REP-RAB1B complexes from transfected 293 cells Molecular Biology of the Cell High 9437002
1999 Correct intracellular localization and tight membrane association of RAB1B depends on GDP/GTP exchange; inactive (S22N, N121I) and constitutively active (Q67L) mutants are not tightly integrated into target membranes in BHK cells. Expression of RAB1B mutants in BHK cells; subcellular localization by immunofluorescence; membrane association assay; co-expression with Mss4 International Journal of Oncology Medium 10493955
2001 RAB1B interacts specifically with the Golgi matrix protein GM130 in a GTP-dependent manner, requiring the hypervariable regions of the N- and C-termini of RAB1B; the RAB1B binding site on GM130 is distinct from the p115 and Grasp65 binding sites. Yeast two-hybrid screen; in vitro binding assays; deletion and mutagenesis mapping EMBO Reports Medium 11306556
2003 Inactive RAB1B (N121I mutant) blocks ER-to-Golgi transport and induces Golgi disruption by compromising COPI recruitment (release of β-COP into cytosol); this phenotype can be rescued by expressing ARF1 or its GEF GBF1, placing RAB1B upstream of ARF1/GBF1-mediated COPI recruitment. The active Q67L mutant confers resistance to BFA-induced Golgi disruption. Expression of RAB1B dominant-negative and constitutively active mutants in cells; immunofluorescence; subcellular fractionation for β-COP; BFA treatment; co-expression rescue experiments Molecular Biology of the Cell High 12802079
2007 Active RAB1B (Q67L) increases GBF1 and COPI association at ER exit sites and stabilizes ARF1 on Golgi membranes; GBF1 is identified as a RAB1B effector via its N-terminal domain; RAB1B siRNA reduces GBF1 membrane association; FRAP shows rapid RAB1B cycling at the Golgi (t½ ~120 s) with minimal microtubule dependence. Dominant mutant expression; co-immunoprecipitation; siRNA knockdown; live-cell time-lapse microscopy; FRAP Molecular Biology of the Cell High 17429068
2010 The Legionella effector DrrA AMPylates RAB1B at Tyr77 (switch II region), covalently attaching AMP; this modification restricts GTPase-activating protein access, rendering RAB1B constitutively active. DrrA also acts as a guanine nucleotide exchange factor for RAB1B. Biochemical AMPylation assay with purified proteins; mass spectrometry identification of modification site; GEF activity assay; GAP accessibility assay Science High 20651120
2010 RAB1B is required for ER-to-Golgi transport of Ebolavirus matrix protein VP40, and dominant-negative RAB1B interferes with VP40-mediated particle formation; this occurs through the RAB1B→GBF1→ARF1→COPI pathway. Dominant-negative RAB1B expression; VP40 particle formation assay; GBF1 and ARF1 inhibition studies Journal of Virology Medium 20164217
2010 RAB1B interacts with COPII components Sec23, Sec24, and Sec31 and modulates COPII assembly/disassembly kinetics at ER exit sites; RAB1B inhibition (by dominant-negative or siRNA) changes COPII phenotype and delays cargo sorting at ER exit sites, as measured by FRAP. Co-immunoprecipitation; siRNA knockdown; dominant-negative mutant expression; FRAP at ER exit sites; cargo transport assay European Journal of Cell Biology Medium 21093099
2012 MTMR6 (phosphatidylinositol 3-phosphatase) preferentially interacts with GDP-bound RAB1B via its GRAM domain and partially colocalizes with RAB1B in pericentrosomal and peri-Golgi regions; RAB1B regulates the localization of MTMR6, and MTMR6 reduction accelerates VSV-G transport (a RAB1B-dependent process); both RAB1B and MTMR6 are required for omegasome tubule formation in autophagy. Co-immunoprecipitation with GDP/GTP-locked RAB1B mutants; siRNA knockdown of RAB1B and MTMR6; immunofluorescence colocalization; VSV-G transport assay; DFCP1-induced omegasome assay The Journal of Biological Chemistry Medium 23188820
2013 Legionella LepB inactivates RAB1B by acting as a GTPase-activating protein (GAP) via an atypical RabGAP mechanism reminiscent of classical GAPs (distinct from mammalian TBC-like GAPs); the crystal structure of the RAB1B:LepB complex reveals an unusual fold in the GAP domain. Crystal structure determination of RAB1B:LepB complex; biochemical GAP activity assays; mutagenesis EMBO Reports High 23288104
2014 The vesicle docking protein p115 enhances RAB1B activation and membrane association: p115 binds RAB1B through its cc2 domain, p115 inhibition causes RAB1B dissociation from Golgi membranes, and constitutively active RAB1B suppresses the COPI recruitment defect caused by p115 inhibition, establishing p115 as a functional upstream activator of RAB1B in COPI recruitment. siRNA knockdown of p115; dominant-active RAB1B rescue experiments; co-immunoprecipitation domain mapping; immunofluorescence for COPI and RAB1B localization Cellular Logistics Medium 25332841
2015 Loss of RAB1B leads to elevated TGF-β receptor 1 (TβR1) levels through decreased ubiquitin-dependent degradation, increased phospho-SMAD3, and TGF-β-induced EMT, revealing that RAB1B acts upstream of TGF-β/SMAD signaling as a metastasis suppressor. siRNA knockdown and overexpression of RAB1B in TNBC cell lines; Western blotting for TβR1, pSMAD3; ubiquitination assay; in vitro migration/invasion assays; in vivo xenograft Oncotarget Medium 25970785
2015 Host RAB1B is recruited to the Yersinia-containing vacuole (YCV) and is required for Y. pestis evasion of phagosome maturation; RAB1B knockdown prevents YCV acidification inhibition and alters LAMP1 association with the YCV, demonstrating RAB1B recruitment is a mechanism by which Y. pestis subverts phagosomal killing. siRNA/shRNA knockdown of RAB1B in macrophages; immunofluorescence for RAB1B on YCV; phagosomal pH measurement; LAMP1 colocalization assay; bacterial survival assay PLoS Pathogens Medium 26495854
2016 PITPNC1 promotes malignant secretion by binding Golgi-resident PI4P and localizing RAB1B to the Golgi; RAB1B localization to the Golgi recruits GOLPH3, which facilitates Golgi extension and enhanced vesicular release of pro-invasive factors. Biochemical PI4P binding assay; RAB1B localization by immunofluorescence; GOLPH3 co-immunoprecipitation; vesicular release assay; siRNA knockdown Cancer Cell Medium 26977884
2016 Adenylylation of Tyr77 of RAB1B by DrrA stabilizes the active (GTP-like) conformation independently of bound nucleotide, primarily through electrostatic effects of the additional negative charge in the switch II region; Phe45-adenine stacking has only minor influence. Molecular dynamics simulation; advanced sampling simulations Scientific Reports Low 26818796
2016 RAB1B dynamically associates with ER exit sites, VTCs, and the Golgi complex; RAB1B dwell time at ER exit sites is regulated by GBF1, and RAB1B membrane cycling kinetics at ERES are dependent on GBF1 membrane association and activity, as shown by live-cell dual-expression imaging and BFA washout experiments. Live-cell dual-color fluorescence microscopy; FRAP; brefeldin A washout cargo-sorting assay; GBF1 dominant-negative and siRNA experiments PLoS One Medium 27500526
2017 RAB1B is associated with ATG9A-containing vesicles in mammalian cells; RAB1B knockdown suppresses autophagy and causes ATG9A accumulation at intermediate membrane structures at autophagosome formation sites, demonstrating RAB1B is required for proper ATG9A vesicle dynamics during autophagosome formation. Immunoisolation of ATG9A vesicles followed by proteomics; siRNA knockdown of RAB1B; fluorescence microscopy of ATG9A and autophagy markers; autophagy flux assay FASEB Journal Medium 28522593
2017 Staphylococcus aureus alpha-hemolysin induces formation of dynamic tubular structures (Saf) labeled with RAB1B, RAB7, and LC3; these tubules emerge from the S. aureus-containing phagosome and their formation depends on RAB1B activity, microtubule integrity, Kinesin-1, and RILP. Live-cell imaging; dominant-negative RAB1B expression; microtubule disruption; siRNA knockdown of kinesin/RILP; fluorescence microscopy Frontiers in Cellular and Infection Microbiology Medium 29046869
2018 RAB1B is a direct transcriptional target of RUNX1; RUNX1 binds the RAB1B promoter at consensus RUNX1 sites (shown by ChIP and EMSA); RUNX1 knockdown impairs ER-to-Golgi vesicle trafficking and Golgi structure in megakaryocytic cells, and these defects are rescued by RAB1B reconstitution; RAB1B is required for trafficking of von Willebrand factor to α-granules. Chromatin immunoprecipitation; EMSA; luciferase promoter assay with RUNX1 site mutations; siRNA knockdown of RUNX1 and RAB1B; RAB1B rescue experiments; ER-to-Golgi transport assay; vWF trafficking assay Blood Advances High 29632235
2019 RAB1B promotes antiviral innate immune signaling by forming a protein complex with TRAF3 and facilitating the interaction between TRAF3 and MAVS, thereby enhancing RIG-I pathway activation and IFN-β induction; RAB1B deletion dampens IFN-β signaling and increases Zika virus susceptibility. Co-immunoprecipitation; RAB1B overexpression and CRISPR deletion in multiple human cell types; IFN-β luciferase reporter assay; viral infection assay (Zika virus) The Journal of Biological Chemistry Medium 31375559
2019 RAB1B directly binds FMDV IRES RNA and stimulates IRES-mediated translation; IRES-driven RNA localizes in close proximity to RAB1B at the ER-Golgi interface, while dominant-negative RAB1B that disorganizes the Golgi abolishes this colocalization. Proteomics-based IRES interactor identification; direct RNA binding assay; IRES translation assay; RNA-FISH imaging with RAB1B colocalization Life Science Alliance Medium 30655362
2020 Human GOLPH3 is a bona fide effector of RAB1B; GOLPH3 interacts directly with RAB1B in a nucleotide-dependent manner favoring the GTP-locked active state; expression of GTP-locked RAB1B variants reduces GOLPH3 distribution at the Golgi apparatus. Co-immunoprecipitation with GTP/GDP-locked RAB1B mutants; direct binding assay; immunofluorescence for GOLPH3 localization PLoS One Medium 32790781
2023 The Legionella enzyme Lem3 acts as a dephosphocholinase on RAB1B, hydrolytically removing the phosphocholine moiety added by AnkX at Ser76; the crystal structures of Lem3 alone and in complex with RAB1B reveal that Lem3 acts by locally unfolding RAB1B at the switch II region and that Lem3 shares structural similarity with metal-dependent protein phosphatases. Crystal structure determination of Lem3 apo and Lem3:RAB1B complex (covalent capture); enzymatic dephosphocholination assay; structural analysis Nature Communications High 37076474
2024 RAB1B promotes DGAT2 redistribution from the ER to the lipid droplet surface (shown by FRET between DGAT2 and RAB1B activity mutants), thereby facilitating lipid droplet growth; TBC1D20 (the RAB1B GAP mutated in Warburg Micro syndrome) loss-of-function alters LD metabolism and DGAT2 redistribution consistently with elevated RAB1B activity. FRET between DGAT2 and RAB1B activity mutants; dominant-negative RAB1B overexpression to block LD formation; TBC1D20 mutant fibroblasts from WARBM model mice; fluorescence microscopy Science Advances Medium 38809969
2025 TBC1D22B is a GAP that directly targets RAB1B; TBC1D22B overexpression inhibits ER-to-Golgi transport in a GAP-activity-dependent manner, and RAB1B silencing phenocopies TBC1D22B-induced trafficking defects, placing RAB1B as the direct substrate of TBC1D22B in ER-to-Golgi transport regulation. Proximity-labeling and co-immunoprecipitation proteomics; RUSH system ER-to-Golgi transport assay; RAB1B siRNA knockdown; GAP-dead mutant rescue experiments Advanced Science Medium 40878439

Source papers

Stage 0 corpus · 45 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1991 Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. The Journal of cell biology 358 1918138
2010 The Legionella effector protein DrrA AMPylates the membrane traffic regulator Rab1b. Science (New York, N.Y.) 301 20651120
2016 PITPNC1 Recruits RAB1B to the Golgi Network to Drive Malignant Secretion. Cancer cell 117 26977884
2003 COPI recruitment is modulated by a Rab1b-dependent mechanism. Molecular biology of the cell 116 12802079
2007 Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association. Molecular biology of the cell 115 17429068
2001 The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b. EMBO reports 108 11306556
1996 Association of Rab1B with GDP-dissociation inhibitor (GDI) is required for recycling but not initial membrane targeting of the Rab protein. The Journal of biological chemistry 71 8631911
1995 The Ras-related GTP-binding protein, Rab1B, regulates early steps in exocytic transport and processing of beta-amyloid precursor protein. The Journal of biological chemistry 59 7738040
2017 Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 56 28522593
2013 Mechanism of Rab1b deactivation by the Legionella pneumophila GAP LepB. EMBO reports 54 23288104
2015 Loss of RAB1B promotes triple-negative breast cancer metastasis by activating TGF-β/SMAD signaling. Oncotarget 45 25970785
2012 Phosphatidylinositol 3-phosphatase myotubularin-related protein 6 (MTMR6) is regulated by small GTPase Rab1B in the early secretory and autophagic pathways. The Journal of biological chemistry 42 23188820
2015 Yersinia pestis Requires Host Rab1b for Survival in Macrophages. PLoS pathogens 36 26495854
1993 Isoprenylation of Rab1B is impaired by mutations in its effector domain. The Journal of biological chemistry 34 8325834
2010 Role of Rab1b in COPII dynamics and function. European journal of cell biology 27 21093099
1998 The putative "switch 2" domain of the Ras-related GTPase, Rab1B, plays an essential role in the interaction with Rab escort protein. Molecular biology of the cell 27 9437002
1989 Biochemical properties of the YPT-related rab1B protein. Comparison with rab1A. FEBS letters 25 2509243
2010 Role of the GTPase Rab1b in ebolavirus particle formation. Journal of virology 24 20164217
2019 The small GTPase RAB1B promotes antiviral innate immunity by interacting with TNF receptor-associated factor 3 (TRAF3). The Journal of biological chemistry 23 31375559
2017 Staphylococcus aureus Alpha-Toxin Induces the Formation of Dynamic Tubules Labeled with LC3 within Host Cells in a Rab7 and Rab1b-Dependent Manner. Frontiers in cellular and infection microbiology 23 29046869
2016 Type II Secretion Is Necessary for Optimal Association of the Legionella-Containing Vacuole with Macrophage Rab1B but Enhances Intracellular Replication Mainly by Rab1B-Independent Mechanisms. Infection and immunity 23 27600508
2014 Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues. International journal of oncology 21 25339198
2019 Rab1b and ARF5 are novel RNA-binding proteins involved in FMDV IRES-driven RNA localization. Life science alliance 16 30655362
2018 Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor. Blood advances 14 29632235
2016 Adenylylation of Tyr77 stabilizes Rab1b GTPase in an active state: A molecular dynamics simulation analysis. Scientific reports 13 26818796
1996 Prenylation of a Rab1B mutant with altered GTPase activity is impaired in cell-free systems but not in intact mammalian cells. The Biochemical journal 13 8836150
2020 Human Golgi phosphoprotein 3 is an effector of RAB1A and RAB1B. PloS one 12 32790781
2016 Spatial-Temporal Study of Rab1b Dynamics and Function at the ER-Golgi Interface. PloS one 12 27500526
2024 Rab1b facilitates lipid droplet growth by ER-to-lipid droplet targeting of DGAT2. Science advances 11 38809969
2021 Rab1b-GBF1-ARF1 Secretory Pathway Axis Is Required for Birnavirus Replication. Journal of virology 11 34878889
1995 Intracellular transport and maturation of nascent low density lipoprotein receptor is blocked by mutation in the Ras-related GTP-binding protein, RAB1B. Journal of receptor and signal transduction research 11 8673720
2024 SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway. Journal of virology 10 38169281
2020 Rab1b-GBF1-ARFs mediated intracellular trafficking is required for classical swine fever virus replication in swine umbilical vein endothelial cells. Veterinary microbiology 9 32605744
2020 MiR-135a inhibits non-small cell lung cancer progression by suppressing RAB1B expression and the RAS pathway. Aging 9 32710726
1999 Inactive and active mutants of rab1b are not tightly integrated into target membranes. International journal of oncology 9 10493955
2023 LINC00173 facilitates tumor progression by stimulating RAB1B-mediated PA2G4 and SDF4 secretion in nasopharyngeal carcinoma. Molecular oncology 8 36606322
2014 Binding of the vesicle docking protein p115 to the GTPase Rab1b regulates membrane recruitment of the COPI vesicle coat. Cellular logistics 8 25332841
2023 Inhibition of Rab1B Impairs Trafficking and Maturation of SARS-CoV-2 Spike Protein. Viruses 7 37112806
2023 Circ_RBM23 knockdown suppresses chemoresistance, proliferation, migration and invasion of sorafenib-resistant HCC cells through miR-338-3p/RAB1B axis. Pathology, research and practice 6 37075641
2023 Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B in Toxoplasma gondii. Parasites & vectors 4 37941035
2008 Rab1b silencing using small interfering RNA for analysis of disease-specific function. Methods in enzymology 4 18413237
2025 TBC1D22B Regulates ER-to-Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 3 40878439
2023 Dephosphocholination by Legionella effector Lem3 functions through remodelling of the switch II region of Rab1b. Nature communications 2 37076474
2021 Influence of a Ser111-phosphorylation on Rab1b GTPase conformational dynamics studied by advanced sampling simulations. Proteins 1 34056776
2025 A guide to selecting high-performing antibodies for Rab1A and Rab1B for use in Western Blot, immunoprecipitation and immunofluorescence. F1000Research 0 38559361

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