Affinage

ARF1

ADP-ribosylation factor 1 · UniProt P84077

Length
181 aa
Mass
20.7 kDa
Annotated
2026-06-09
100 papers in source corpus 63 papers cited in narrative 63 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARF1 is a myristoylated small GTPase that functions as a master organizer of membrane trafficking, cytoskeletal remodeling, and organelle homeostasis by cycling between an inactive cytosolic GDP state and an active membrane-inserted GTP state (PMID:8106346, PMID:19141284). N-myristoylation at Gly2 is required for ARF1 function and stable membrane association in its GTP form, while activation is catalyzed by RCC1-like and Sec7-domain GEFs that prime ARF1 for membrane insertion through GEF-domain conformational changes (PMID:7814365, PMID:8861955, PMID:36044848); the cycle is terminated by zinc-finger ArfGAPs whose recruitment is itself ARF1- and curvature-dependent (PMID:8533093, PMID:19927117). In its GTP state ARF1 localizes to the Golgi and recruits the COPI coatomer through bivalent GTP-dependent contacts with the γζ- and βδ-COP subcomplexes plus a δ-COP longin-domain interface, and GTP-induced ARF1 dimerization generates the positive membrane curvature that drives COPI vesicle budding (PMID:8106346, PMID:7896867, PMID:17451557, PMID:18689681, PMID:22304919). Beyond coat recruitment, ARF1 nucleates actin polymerization via a CDC42/N-WASP/Arp2/3 cascade and an actin-cortactin-dynamin-2 complex to power vesicle fission and tubular-carrier formation, recruits clathrin adaptors AP-1 and AP-3, and orchestrates PI4P production (via the Pik1-Frq1 kinase) and Rab GTPase conversion (via Gyp1 recruitment) on maturing Golgi compartments (PMID:15821732, PMID:17942688, PMID:20228810, PMID:33788598, PMID:33788577). ARF1 maintains two functional classes of trafficking compartments—perinuclear stations for Golgi export and peripheral compartments for AP-1-dependent endocytic recycling—and supports recycling-endosome dynamics together with ARF3 and ARF4 (PMID:22971977, PMID:23783033, PMID:39367144). ARF1 also regulates lipid-droplet morphology, ER-LD bridge formation, and the targeting of lipid enzymes to droplet surfaces, and controls Golgi positioning and mitochondrial transport through nucleotide-dependent interactions with dynein and Miro (PMID:24497546, PMID:30459446, PMID:30054383). Through downstream RhoA, Rac1, and ERK signaling and direct integrin/FAK/paxillin coordination at focal adhesions, ARF1 drives cell adhesion, spreading, invasion, and proliferation, and its activity is gated by upstream EGFR/Grb2 signaling, cell-matrix adhesion, intracellular pH, and lipid composition (PMID:24196838, PMID:23707487, PMID:25530216, PMID:24407288, PMID:28007915). Heterozygous GTPase-defective ARF1 missense mutations cause a type I interferonopathy by perturbing mitochondrial integrity, driving mtDNA release with cGAS activation, and trapping active STING at the Golgi/ERGIC due to defective retrograde transport (PMID:37914730).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 1995 High

    Established that ARF1 is a Golgi-localized GTPase whose GTP state, not ARF6's, drives membrane recruitment of coat machinery, defining its core trafficking role and distinguishing it from a paralog.

    Evidence In vitro coatomer-binding assay with the hydrolysis-defective Q71I mutant plus immunofluorescence/immuno-EM with dominant-negative and constitutively active ARF mutants

    PMID:7896867 PMID:8106346

    Open questions at the time
    • Did not resolve the molecular contacts between ARF1 and coatomer
    • Did not address ARF1 functions outside COPI coat recruitment
  2. 1995 High

    Defined the determinants of the ARF1 nucleotide cycle by showing myristoylation is required for in-cell function while Asp26 governs activating-nucleotide binding, and by cloning a zinc-finger ArfGAP recruited to the Golgi in an ARF1-dependent manner.

    Evidence Site-directed mutagenesis with yeast complementation and in vitro ARF activity assays; ArfGAP cDNA cloning with zinc-finger mutants and BFA-sensitive fractionation

    PMID:7814365 PMID:8533093

    Open questions at the time
    • Structural basis of myristoyl-switch membrane insertion not resolved
    • Substrate specificity of the GAP toward ARF paralogs not fully defined
  3. 2002 High

    Distinguished ARF1 and coatomer kinetics on membranes, showing ARF1 cycles rapidly and stochastically and that coat persistence outlasts ARF1-GTP, refining the coupling between the GTPase cycle and coat dynamics.

    Evidence FRAP of fluorescent coatomer and ARF1-GFP in living cells with quantitative imaging

    PMID:12000962

    Open questions at the time
    • Did not capture the curvature-generation step of budding
    • Did not address ARF1 functions away from the Golgi coat
  4. 2003 High

    Connected ARF1 to mitotic Golgi inheritance and cytokinesis, showing that maintaining ARF1-GTP prevents Golgi disassembly and produces chromosome-segregation and furrow defects.

    Evidence Live-cell imaging with pharmacological (H89) and constitutively active ARF1 perturbations

    PMID:14585930

    Open questions at the time
    • Direct effectors linking ARF1 to the cytokinetic machinery not identified
    • Whether the defects are coat-dependent unresolved
  5. 2005 High

    Showed ARF1 is an upstream activator of cytoskeletal machinery at the Golgi by recruiting an actin-cortactin-dynamin-2 complex required for post-Golgi cargo transport, extending ARF1 beyond coat recruitment.

    Evidence In vitro Golgi membrane and intact-cell assays with co-IP and dominant-negative disruption of cortactin-Dyn2

    PMID:15821732

    Open questions at the time
    • How ARF1 nucleates the actin complex mechanistically not defined
    • Relationship to the later CDC42/N-WASP pathway not yet integrated
  6. 2007 High

    Provided the structural basis of ARF1 effector engagement and demonstrated ARF1-GTP directly drives actin polymerization, establishing both how ARF1 binds effectors and that it generates mechanical force for vesicle fission.

    Evidence X-ray crystallography of ARF1-GTP with the ARHGAP21 Arf-binding domain plus mutagenesis; biomimetic liposome actin-comet assay with CDC42/N-WASP/Arp2/3 dissection

    PMID:17347647 PMID:17942688

    Open questions at the time
    • Generality of the switch-region binding mode to other effectors not established
    • In vivo contribution of comet-tail force to physiological budding unquantified
  7. 2008 High

    Identified GTP-induced ARF1 dimerization as the mechanism generating membrane curvature for COPI vesicle formation, linking the nucleotide state directly to membrane deformation.

    Evidence In vitro membrane curvature and COPI budding assays with a dimerization-defective mutant and yeast viability test

    PMID:18689681

    Open questions at the time
    • Structural detail of the dimer interface on membranes not resolved
    • How dimerization is coordinated with coat assembly in cells unclear
  8. 2012 High

    Resolved the bivalent, two-site GTP-dependent mode by which two ARF1-GTP molecules engage the γζ- and βδ-COP subcomplexes, providing the definitive structural logic of coatomer recruitment.

    Evidence X-ray crystallography with structure-guided mutagenesis and biochemical binding assays

    PMID:17451557 PMID:22304919

    Open questions at the time
    • Did not capture the assembled coat on a curved membrane
    • Stoichiometry on native Golgi membranes not measured
  9. 2012 High

    Showed that GEF localization spatially patterns ARF1 activity, with Arl1 directing BIG1/BIG2-mediated activation to the trans-Golgi while GBF1 acts elsewhere, explaining compartment-specific ARF1 function.

    Evidence Liposome-based affinity purification and direct binding with Arl1 knockdown and immunofluorescence

    PMID:22291037

    Open questions at the time
    • How distinct GEF pools generate distinct effector outputs not fully mapped
    • Mammalian generality of the Arl1-Sec71 interaction partially inferred from Drosophila
  10. 2010 High

    Integrated ARF1 with clathrin-AP-1 coats and Rac1/PIX-driven N-WASP/Arp2/3 actin polymerization to form Golgi tubular carriers, unifying coat recruitment and cytoskeletal force in carrier biogenesis.

    Evidence Co-IP, reconstitution on synthetic membranes, siRNA, and live-cell imaging

    PMID:20228810

    Open questions at the time
    • Quantitative contribution of actin versus coat to scission not separated
    • How carrier identity is selected not resolved
  11. 2014 High

    Extended ARF1 function to lipid-droplet biology, showing ARF1/COPI bud nano-LDs, target TG-synthesis enzymes to LD surfaces, and control LD surface tension and ER-LD bridge formation.

    Evidence Live-cell and super-resolution imaging, in vitro nano-LD budding, phospholipid quantification, and ER-LD contact analysis; GBF1/ATGL interaction studies

    PMID:21789191 PMID:24497546

    Open questions at the time
    • Whether nano-LD budding uses the same dimerization mechanism as Golgi budding untested
    • Physiological regulation of LD-targeted ARF1 not defined
  12. 2013 High

    Defined ARF1 as an upstream regulator of Rho-family GTPase signaling in invasion and synaptic plasticity, controlling RhoA/RhoC, Rac1, and PICK1/Arp2/3 to drive invadopodia, lamellipodia, and AMPAR internalization.

    Evidence siRNA, dominant-negative/constitutively active ARF1, direct interaction assays, FRET GTPase assays, and LTD electrophysiology

    PMID:23707487 PMID:23889934 PMID:24196838

    Open questions at the time
    • Whether these signaling roles require ARF1 membrane-trafficking activity not disentangled
    • Direct versus indirect coupling to each Rho GTPase partly inferred
  13. 2014 High

    Mapped upstream control of ARF1 activation by growth-factor and adhesion signaling and by PI4P coincidence detection, showing Grb2 promotes and p66Shc blocks ARF1 recruitment to EGFR and that ARF1-GTP plus PI4P recruit FAPP1.

    Evidence Co-IP, siRNA, ARF1 activation assays, integrin coordination assays, and NMR interaction mapping with FAPP1 PH domain

    PMID:24407288 PMID:24462251 PMID:25530216

    Open questions at the time
    • Quantitative thresholds for coincidence detection in cells not defined
    • How adhesion signaling biochemically sets ARF1-GTP levels not fully resolved
  14. 2015 High

    Revealed how viral and host factors hijack and bound ARF1 activity, with Nef-ARF1 organizing AP-1 trimerization for clathrin coat assembly and GIV/Gαi imposing finiteness on the ARF1 cycle.

    Evidence Cryo-EM of the Nef-ARF1-AP-1 trimer with in vitro clathrin cage reconstitution; co-IP with ARF1-GTP loading and transport assays

    PMID:25865347 PMID:26494761

    Open questions at the time
    • Physiological (non-Nef) trigger for AP-1 trimerization not established
    • How GIV/Gαi feedback is coordinated with GAPs unclear
  15. 2018 High

    Demonstrated tissue-level ARF1 functions and effector selectivity in vivo, with BIG1-ARF1 selectively driving AP-1 engagement for Schwann cell myelination and BIG2-ARF1-RhoA-mDia1 controlling neuronal Golgi deployment.

    Evidence Conditional knockout mice with EM and co-IP dissection of coat-complex binding; siRNA, constitutively active ARF1 rescue, and in utero electroporation

    PMID:29455446 PMID:29740613

    Open questions at the time
    • How specific GEFs bias ARF1 toward AP-1 versus COPI mechanistically not resolved
    • Cell-type generality of GEF-defined effector selectivity untested
  16. 2018 Medium

    Connected ARF1 to mitochondrial positioning, showing GBF1 and ARF1-GTP interact with Miro and restrain dynein-dependent retrograde mitochondrial transport.

    Evidence Co-IP with live-cell mitochondrial tracking under GBF1/ARF1 inhibition, Miro siRNA, and dynein inhibition

    PMID:30054383 PMID:30459446

    Open questions at the time
    • Whether ARF1-Miro coupling is direct not established
    • Single-lab co-IP without reciprocal structural validation
  17. 2021 High

    Established ARF1 as a master coordinator of Golgi maturation by directly recruiting the Pik1-Frq1 PI4-kinase for PI4P production and recruiting Gyp1 to drive Rab1 inactivation and Rab conversion.

    Evidence In vitro reconstitution on Golgi-mimetic membranes with acute PI4P depletion and live-cell imaging; yeast genetics and live-cell imaging of Rab conversion

    PMID:33788577 PMID:33788598

    Open questions at the time
    • Mammalian conservation of the Gyp1 recruitment mechanism not directly shown
    • Temporal ordering of PI4P production versus Rab conversion not fully resolved
  18. 2022 High

    Provided a structural mechanism for ARF1 activation by capturing the GEF in two conformations and a Gea2-ARF1 intermediate, showing how GEF-domain movement primes ARF1 for membrane insertion on GTP binding.

    Evidence CryoEM of full-length Gea2 and the Gea2-ARF1 activation intermediate

    PMID:36044848

    Open questions at the time
    • Membrane-embedded activation step not directly visualized
    • Regulation of GEF conformational switching by upstream signals unresolved
  19. 2024 High

    Resolved ARF1 compartments into two functional classes and showed AP-1-dependent maturation into recycling endosomes, defining ARF1's role across both secretory export and endocytic recycling.

    Evidence CRISPR endogenous tagging with fast confocal, STED, CLEM, and AP-1 depletion

    PMID:28428254 PMID:39367144

    Open questions at the time
    • Molecular cues distinguishing perinuclear versus peripheral compartment fate not identified
    • How coat-independent tubular carriers relate to these compartments unclear
  20. 2023 High

    Linked ARF1 to innate-immune and metabolic homeostasis, showing GTPase-defective ARF1 mutations cause a type I interferonopathy via disrupted mitochondrial integrity, mtDNA release, and defective STING retrograde transport, and that hyperactive ARF1 dysregulates fatty acid storage and mitochondrial function.

    Evidence Patient-derived cells with ISG, mitochondrial morphology, mtDNA release, and STING trafficking assays; yeast hyperactive mutant with transcriptomics, LD staining, and ATP measurement

    PMID:31924786 PMID:37400497 PMID:37914730

    Open questions at the time
    • How a single GTPase coordinates STING transport and mitochondrial integrity mechanistically not unified
    • Therapeutic correction of the dual defect not addressed
  21. 2024 Medium

    Identified ARF1 lactylation as a metabolically controlled PTM, with astrocytic LRP1 suppressing lactate to limit ARF1 lactylation and preserve mitochondria transfer to neurons.

    Evidence LRP1 knockdown with ARF1 lactylation detection, mitochondria-transfer assay, and a mouse ischemia-reperfusion model

    PMID:38906140

    Open questions at the time
    • Lactylation site and its effect on ARF1 nucleotide cycling not defined
    • Single-lab functional study without structural mechanism

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single GTPase selects among its many effectors (COPI, AP-1/AP-3, PI4-kinase, Rab-GAPs, actin nucleators, dynein/Miro) at distinct compartments, and how upstream signals (lipid composition, pH, PTMs, adhesion) are biochemically transduced into spatially patterned ARF1 activity, remains unresolved.
  • No unified model coupling GEF/GAP identity to effector choice at each compartment
  • Effect of lactylation and phosphorylation-linked regulation on the nucleotide cycle not mechanistically defined
  • Structural picture of ARF1 acting in coat-independent tubular carriers absent

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 5 GO:0060089 molecular transducer activity 4 GO:0060090 molecular adaptor activity 3 GO:0008289 lipid binding 2
Localization
GO:0005794 Golgi apparatus 6 GO:0005768 endosome 5 GO:0005739 mitochondrion 3 GO:0005811 lipid droplet 3 GO:0005886 plasma membrane 3 GO:0005829 cytosol 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 6 R-HSA-162582 Signal Transduction 5 R-HSA-168256 Immune System 4 R-HSA-9609507 Protein localization 4 R-HSA-1430728 Metabolism 3 R-HSA-1640170 Cell Cycle 2
Partners
AP1ARHGAP21ASAP1COPB1GBF1IQGAP1MIROPICK1
Complex memberships
COPI coatomerclathrin-AP-1 adaptor coat

Evidence

Reading pass · 63 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 ARF1-GTP state supports the binding of coatomer (COPI) to Golgi membranes; the activating Q71I mutation slows GTP hydrolysis and stabilizes coatomer binding, providing evidence that ARF1 GTPase cycling regulates reversible coat protein association with the Golgi. In vitro coatomer-binding assay with GTP hydrolysis-defective ARF1 mutant (Q71I); BFA-resistance assay in cells The Journal of biological chemistry High 8106346
1995 ARF1 localizes to the Golgi complex and mediates membrane recruitment of the coat proteins coatomer (COP1) and gamma-adaptin; GTP-state ARF1 is required for this association, distinct from ARF6 which acts at the endosomal/plasma membrane system. Transient transfection with epitope-tagged ARFs; immunofluorescence; immuno-electron microscopy; dominant-negative and constitutively active ARF mutants The Journal of cell biology High 7896867
1995 An ARF1 GTPase-activating protein (GAP) was cloned from rat liver; it contains a zinc finger motif required for GAP activity, localizes to the Golgi complex, and redistributes to cytosol upon brefeldin A treatment, indicating it is recruited to the Golgi by an ARF1-dependent mechanism. cDNA cloning; in vitro GAP assay with zinc-finger mutants; subcellular fractionation; BFA treatment Science High 8533093
1995 N-myristoylation of ARF1 (at Gly2) is required for ARF1 function in cells but not for nucleotide exchange or cofactor activities in vitro; Asp26 is essential for binding activating nucleotide GTPγS, unlike the homologous residue in Ras. Site-directed mutagenesis; yeast complementation; in vitro ARF activity assay The Journal of biological chemistry High 7814365
1996 The giant protein p619 stimulates guanine nucleotide exchange (GEF activity) on myristoylated ARF1 and Rab proteins via its RCC1-like domain; it localizes to the Golgi in a BFA-sensitive manner and interacts specifically with myristoylated ARF1. In vitro nucleotide exchange assay; GST pull-down; subcellular localization; BFA treatment The EMBO journal Medium 8861955
1997 The KDEL receptor ERD2 self-oligomerizes and interacts with ARF1 GAP, regulating ARF1 GAP recruitment to membranes; ERD2 overexpression enhances GAP membrane recruitment and produces an ARF1-inactivation phenotype, linking KDEL receptor signaling to ARF1 GTPase cycling. Co-immunoprecipitation; overexpression studies; dominant-negative ARF1 phenotype analysis The EMBO journal Medium 9405360
2000 ARF1 is required for membrane recruitment of endosomal COP proteins and for in vitro biogenesis of transport intermediates destined for late endosomes; ARF1 membrane association in endosomes is regulated by endosomal pH, providing a transmembrane pH-sensing mechanism. In vitro endosome budding assay; cytosol fractionation; dominant-negative ARF1; pH manipulation The Journal of biological chemistry Medium 10713138
2001 DEF-1/ASAP1 functions as a GAP specifically for ARF1 (not ARF6) in vivo; DEF-1-mediated ARF1 deactivation enhances cell motility in a GAP-domain-dependent manner. Cell-based ARF GAP assay; stable cell line overexpression; GAP-domain mutant; cell migration assay The Journal of biological chemistry Medium 11773070
2001 Yeast Arf1 is involved in multiple distinct steps of intracellular transport; different temperature-sensitive arf1 alleles produce distinct transport defects and morphological alterations, demonstrating that Arf1 has separable functions at multiple trafficking steps. Error-prone PCR mutagenesis; yeast genetics; transport assays (CPY, invertase); intragenic complementation Molecular biology of the cell Medium 11160834
2002 Activated Arf1-GTP recruits coatomer to Golgi membranes; once membrane-associated, coatomer has a longer residence time than Arf1 and persists after Arf1-GTP hydrolysis and dissociation; Arf1 and coatomer cycle rapidly and stochastically on membranes even without vesicle budding. FRAP of fluorescently labeled coatomer and Arf1-GFP in living cells; quantitative live-cell imaging Nature High 12000962
2002 Genetic interaction screen in yeast identified that ARF1 and TRS130 (TRAPP complex component) are synthetically lethal; YPT31/32 high-copy suppresses arf1Δtrs130 lethality; these genetic interactions link Arf1 and TRAPP signaling. Yeast synthetic lethal screen; high-copy suppressor screen; yeast genetics Yeast Medium 12210902
2003 Arf1 inactivation early in mitosis leads to dissociation of peripheral Golgi proteins and subsequent Golgi disassembly; maintaining Arf1 in the active GTP-bound state (via H89 treatment or GTP-locked expression) prevents Golgi disassembly and causes defects in chromosome segregation and cytokinetic furrow ingression. Live-cell imaging; GFP-Arf1 overexpression; pharmacological activation (H89); constitutively active ARF1 expression; quantitative microscopy Proceedings of the National Academy of Sciences of the United States of America High 14585930
2003 ARF1 directly binds the carboxy-terminal tail domain of the 5-HT2A receptor in a GTP-enhanced manner; ARF1 plays a greater role than ARF6 in 5-HT2AR-dependent phospholipase D activation; the N376PxxY motif in the receptor is essential for ARF-dependent PLD signaling. GST pull-down with receptor domain fusions; co-immunoprecipitation; dominant-negative ARF constructs; in vitro GTPγS-enhanced binding Molecular pharmacology Medium 14573774
2003 Pyk2 tyrosine kinase phosphorylates ASAP1 (an ARF GAP) on Tyr308 and Tyr782, inhibiting ASAP1 GAP activity toward Arf1, thereby increasing Arf1-GTP levels; Pyk2 interacts with ASAP1 via proline-rich regions of Pyk2 and the SH3 domain of ASAP1. Yeast two-hybrid; co-immunoprecipitation; in vitro kinase and GAP assays; fluorimetric Arf-GTPase assay; phosphopeptide mapping The Journal of biological chemistry High 12771146
2004 HIV-1 Nef directly binds ARF1 and recruits it to endosomal membranes; a complex of Nef, ARF1, and βCOP can be immunoprecipitated; dominant-negative ARF1 blocks migration of Nef-CD4 complex to lysosomes, establishing ARF1 as the immediate downstream partner of Nef for CD4 lysosomal targeting. Direct binding assay; co-immunoprecipitation; dominant-negative ARF1; CD4 trafficking assay Current biology Medium 15202998
2004 ARF1 (GDP-bound form) directly interacts with adipocyte differentiation-related protein (ADRP) on lipid droplets; GDP-ARF1 induces dissociation of ADRP from lipid droplet surfaces; BFA treatment or dominant-negative ARF1 causes ADRP dissociation. Yeast two-hybrid; GST pull-down; co-immunoprecipitation; BFA treatment; dominant-negative ARF1 overexpression Biochemical and biophysical research communications Medium 15336557
2005 Arf1 activation mediates the recruitment of actin, cortactin, and dynamin-2 (Dyn2) to Golgi membranes; disruption of the cortactin-Dyn2 interaction reduces Dyn2 at the Golgi and blocks trans-Golgi network protein transport, establishing Arf1 as an upstream activator of an actin-cortactin-Dyn2 complex essential for post-Golgi transport. In vitro Golgi membrane assay; intact-cell experiments; co-immunoprecipitation; dominant-negative disruption of cortactin-Dyn2 interaction; fluorescence microscopy Nature cell biology High 15821732
2005 Mislocalization or siRNA knockdown of ASAP1 (an ARF GAP) inhibits cell spreading and migration and increases GTP loading on Arf1, demonstrating that dynamic Arf1 GTP/GDP cycling (not just a single GTP state) is required for paxillin localization to focal adhesions. siRNA knockdown; mitochondria-targeting mislocalization strategy; GTP-loading assay; cell spreading and migration assays The Journal of biological chemistry Medium 15632162
2005 Mutations in the C-terminal helix of Arf1 (position 167) reveal a novel interaction interface between Arf1-GTP and coatomer via the delta-COP longin domain, in addition to previously described interactions via switch I with beta- and gamma-COP trunk domains. Site-directed photolabeling; site-directed mutagenesis; in vitro binding assay Traffic Medium 17451557
2006 ARF1 is activated during FcγR-mediated phagocytosis via BFA-insensitive GEFs; blocking ARF1 cycling inhibits phagosome closure; ARF1 activation is spatially and temporally downstream of ARF6 activation and depends on PI 3-kinase signaling during phagocytosis. FRET stoichiometric microscopy with CFP/YFP-ARF chimeras; PI 3-kinase inhibition; dominant-negative ARF1; macrophage phagocytosis assay PLoS biology High 16669702
2007 Crystal structure of GTP-bound ARF1 in complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 Å reveals that ARF1 interacts with both a PH domain and an adjoining C-terminal α-helix through its switch regions; site-directed mutagenesis confirmed both structural elements are required for ARF1 binding and Golgi recruitment of ARHGAP21. X-ray crystallography; site-directed mutagenesis; Golgi recruitment assay The EMBO journal High 17347647
2007 ARF1 controls AP-1 (but not AP-2) recruitment to endosomal sites during FcγR-mediated phagocytosis; AP-1 depletion increases surface TNF-α levels; ARF1-dependent AP-1 recruitment supports clathrin-independent endosomal remodeling during phagocytosis. siRNA knockdown of AP-1; dominant-negative ARF1; immunofluorescence; TNF-α surface level assay Molecular biology of the cell Medium 17914058
2007 Activated ARF1 drives actin polymerization on liposomes via a CDC42/N-WASP/Arp2/3 cascade, generating actin comet tails that produce movement of ARF1-carrying vesicles, demonstrating that ARF1 can generate mechanical forces via actin polymerization to contribute to vesicle fission. Biomimetic liposome assay with cell extracts; dominant-negative CDC42; N-WASP inhibition; live-cell actin imaging Proceedings of the National Academy of Sciences of the United States of America High 17942688
2008 Arf1-GTP induces positive membrane curvature and can dimerize in a GTP-dependent manner; an Arf1 dimerization-defective mutant cannot mediate COPI vesicle formation from Golgi membranes and is lethal in yeast, despite retaining coat receptor function, establishing that GTP-induced Arf1 dimerization drives membrane curvature required for vesicle formation. In vitro membrane curvature assay; Arf1 dimerization-defective mutant; COPI vesicle budding assay from Golgi membranes; yeast viability assay Proceedings of the National Academy of Sciences of the United States of America High 18689681
2008 GBF1-mediated ARF1 activation is required for efficient mouse hepatitis coronavirus (MHV) RNA replication; siRNA knockdown of GBF1 or ARF1, or dominant-negative ARF1, significantly inhibits MHV infection; ARF1 inactivation does not block replication complex formation per se but reduces their number. Dominant-negative ARF1 expression; siRNA knockdown of GBF1, BIG1, BIG2, ARF1; BFA treatment; immunofluorescence; quantitative electron microscopy PLoS pathogens High 18551169
2009 Solution NMR structure of myristoylated ARF1 shows that myristoylation contributes to regulation of guanine nucleotide exchange and stable membrane association; ARF1-GTP has greater membrane affinity than ARF1-GDP, with the myristoyl group influencing both. Solution NMR; lipid bilayer binding assay Structure High 19141284
2009 ArfGAP1 ALPS motifs bind preferentially to positively curved membranes (threshold ~35 nm radius); when Arf1-GTP and ArfGAP1 coexist on membrane tubes, ArfGAP1 generates a smooth Arf1-GTP gradient along the tube, allowing Arf1-GTP to diffuse from flat regions to compensate for localized GTP hydrolysis at curved regions. Giant vesicle tube-pulling assay with molecular motors/optical tweezers; quantitative fluorescence microscopy; reconstituted Arf1-GTP/ArfGAP1 system The EMBO journal High 19927117
2010 Arf1 GTPase coordinates TGN association of clathrin-AP-1 coats with CYFIP/Sra/PIR121-containing complexes; Rac1/β-PIX downstream of Arf1 activates N-WASP/Arp2/3-dependent actin polymerization toward membranes, promoting tubule formation; this was recapitulated on synthetic membranes. Co-immunoprecipitation; reconstitution on synthetic membranes; siRNA knockdown; live-cell imaging; dominant-negative mutants Nature cell biology High 20228810
2011 ARF1 directly interacts with GBF1 (the Arf1 exchange factor GEF); GBF1 and ATGL interact directly through multiple contact sites, with GBF1 HDS1/HDS2 domains localizing to lipid droplets when expressed alone; GBF1/Arf1/COPI pathway is required for delivery of the lipase ATGL to lipid droplets. Yeast two-hybrid; co-immunoprecipitation; direct protein binding assay; subcellular localization of domain fragments PloS one Medium 21789191
2011 GDP-bound ARF1 directly interacts with the retinoblastoma protein (pRB) but not other pRB family members; in ARF1-depleted or dominant-negative ARF1-expressing cells, GDP-ARF1 is enriched on chromatin and stabilizes the pRB/E2F1 interaction, preventing E2F target gene expression and arresting cell proliferation. Co-immunoprecipitation; chromatin fractionation; dominant-negative ARF1; siRNA knockdown; ChIP; gene expression analysis Oncogene Medium 21478909
2012 Crystal/structural analysis combined with biochemical studies shows that Arf1-GTP binds the γζ-COP subcomplex of coatomer at one site, and a second Arf1-GTP molecule binds βδ-COP at a site common to both γ- and β-COP subunits; this bivalent GTP-dependent binding mode underlies coatomer recruitment to the Golgi. X-ray crystallography; structure-guided mutagenesis; biochemical binding assays Cell High 22304919
2012 Arl1 is necessary for Golgi recruitment of BIG1 and BIG2 (trans-Golgi-specific ARF1 GEFs) but not GBF1; Arl1 binds directly to Sec71 (Drosophila BIG1/BIG2 ortholog) via an N-terminal region, thereby directing active Arf1 preferentially to the trans-Golgi. Liposome-based affinity purification; direct binding assay; siRNA knockdown of Arl1; immunofluorescence The Journal of cell biology High 22291037
2012 Simultaneous depletion of ARF1 and ARF3 induces tubulation of recycling endosomes and suppresses transferrin recycling from endosomes to the plasma membrane, without affecting retrograde transport from endosomes to the TGN. siRNA double knockdown; fluorescence microscopy; transferrin recycling assay; retrograde transport assay Cell structure and function Medium 22971977
2013 ARF1 acts upstream of RhoA/RhoC to control myosin light chain (MLC) phosphorylation, invadopodia maturation, microvesicle shedding, and MMP-9 activity in invasive breast cancer cells; ARF1 depletion impairs extracellular matrix degradation and cell invasiveness. siRNA knockdown; dominant-negative and constitutively active ARF1; RhoA/RhoC activity assay; MLC phosphorylation assay; invadopodia and matrix degradation assay Molecular biology of the cell Medium 24196838
2013 ARF1-GTP binds PICK1, limiting PICK1-mediated inhibition of Arp2/3 actin polymerization; NMDAR stimulation downregulates Arf1 activation via the Arf-GAP GIT1, releasing PICK1 to inhibit Arp2/3, thereby mediating AMPAR internalization and LTD. Co-immunoprecipitation; dominant-negative Arf1 that does not bind PICK1; FRET-based GTPase assay; organotypic slice LTD recordings; spine morphology analysis Neuron High 23889934
2013 ARF1 controls Rac1 activation downstream of EGF; ARF1 and Rac1 directly interact regardless of nucleotide state; ARF1 is required for plasma membrane targeting of Rac1 and IRSp53 for lamellipodia formation and cell migration in invasive breast cancer cells. siRNA knockdown; direct interaction assay; dominant-negative Rac1 rescue; GTP-loading assay; plasma membrane fractionation Cellular signalling Medium 23707487
2013 Simultaneous depletion of ARF1 and ARF4 induces tubulation of recycling endosomes and inhibits retrograde transport of TGN38 and mannose-6-phosphate receptor from early/recycling endosomes to the TGN, a pathway distinct from ARF1+ARF3-dependent transferrin recycling. siRNA double knockdown; immunofluorescence; transferrin and TGN38 trafficking assays Molecular biology of the cell Medium 23783033
2014 Arf1/COPI proteins localize to cellular lipid droplets (LDs), bud nano-LDs (~60 nm) from LD surfaces, and are required for targeting specific TG-synthesis enzymes to LD surfaces; loss of Arf1/COPI function increases LD phospholipid content, decreasing surface tension and impairing LD-ER bridge formation. Live-cell imaging; super-resolution microscopy; in vitro nano-LD budding assay; phospholipid quantification; ER-LD contact site analysis eLife High 24497546
2014 ARF1 regulates adhesion of invasive breast cancer cells by controlling recruitment of paxillin, talin, and FAK to β1-integrin at focal adhesions; ARF1 can be found in complex with β1-integrin, paxillin, talin, and FAK; ARF1 is essential for EGF-mediated FAK and Src phosphorylation. siRNA knockdown; co-immunoprecipitation; immunofluorescence; focal adhesion assay; phosphorylation assays Cellular signalling Medium 25530216
2014 Adaptor protein Grb2 promotes ARF1 activation and recruits ARF1 to EGFR; p66Shc blocks ARF1 activation and receptor recruitment by preventing Grb2/ARF1 complex formation; ARF1 can be co-immunoprecipitated with both Grb2 and p66Shc upon EGF stimulation. Co-immunoprecipitation; siRNA knockdown; ARF1 activation assay; receptor recruitment assay The Journal of biological chemistry Medium 24407288
2014 NMR-based structural data shows that yeast Arf1 interacts with the PH domain of Fapp1 at a membrane surface through contacts between switch I of Arf1 and regions near the C-terminal extension of the Fapp1 PH domain, with the Arf1-binding site distinct from the PI4P-binding site, supporting coincidence detection of active ARF1 and PI4P for Fapp1 membrane recruitment. Solution NMR with membrane-surface interaction mapping Structure High 24462251
2014 In yeast, Arf1 interacts with the vacuolar ATPase (V-ATPase) and is required for glucose-induced Ras/PKA activation; cytosolic pH acts as a signal linking glucose availability to Ras/PKA through the V-ATPase–Arf1 axis. Genetic epistasis; co-immunoprecipitation; in vivo pH measurement; Ras/PKA activity assays Molecular cell Medium 25002144
2014 ARF1-GTP regulates Asrij endocytic function in Drosophila blood cells; ARF1-GTP is essential for hematopoietic niche size and prohemocyte maintenance; ARF1 perturbation causes aberrant Notch trafficking and stalls the Notch intracellular domain in sorting endosomes. RNA interference in Drosophila lymph gland; GEF knockdown (Gartenzwerg); GAP overexpression; Notch trafficking assay by immunofluorescence; co-immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America Medium 24707047
2014 E. coli EspG scaffolds tether vesicles through selective ARF1-GTP/effector complexes while locally inactivating Rab1, inducing bidirectional ER-Golgi traffic arrest; structural modeling reveals that EspG binds ARF1-GTP specifically. Structural modeling; co-immunoprecipitation; dominant-negative studies; cell-based trafficking assay Cell reports Medium 24582959
2015 HIV-1 Nef and Arf1 together induce trimerization and allosteric activation of AP-1; cryo-EM structures of the Nef- and Arf1-bound AP-1 trimer reveal a central nucleus of three Arf1 molecules organizing the trimers; reconstitution of clathrin cage assembly validated a predicted hexagonal AP-1 coat assembly. Cryo-electron microscopy; in vitro clathrin cage reconstitution; structural validation of hexagonal assembly model Science High 26494761
2015 GIV/Girdin activates Gαi at the Golgi, which interacts with active Arf1, ArfGAP2/3, and β-COP to impose finiteness on Arf1 GTPase cycling; inhibition of the GIV-Gαi pathway elevates GTP-bound Arf1 and delays protein transport along the secretory pathway. Co-immunoprecipitation; GIV-GEF inhibition; ARF1-GTP loading assay; protein transport assay Developmental cell Medium 25865347
2016 Podosome assembly requires the GTPase ARF1 and its GEF ARNO; ARF1 inhibition increases RhoA-GTP levels and triggers myosin-IIA filament assembly, causing podosome elimination; myosin-IIA suppression rescues podosome formation despite ARF1 inhibition; constitutively active ARF1 induces podosome precursor (actin-rich puncta) formation. siRNA knockdown of ARF1 and ARNO; pharmacological inhibitors; constitutively active ARF1 expression; RhoA-GTP assay; myosin-IIA rescue experiment The Journal of cell biology High 28007915
2016 ARF1-mediated MAPK signaling (ERK1/2) in prostate cancer requires Thr48 in ARF1; mutation of Thr48 abolishes ARF1's ability to activate ERK1/2 and promote cell proliferation; ARF1 activity correlates with ERK1/2 phosphorylation and tumor growth in xenograft models. ARF1 overexpression and knockdown; T48 point mutant; Raf1/MEK inhibitors; xenograft mouse model; ERK1/2 phosphorylation assay Oncotarget Medium 27213581
2017 ARF1-GTP is functionally required for formation of long thin (~3 µm, ~110 nm diameter) tubular carriers from the Golgi that carry anterograde and retrograde cargo; these tubules are largely free of COPI and clathrin coat proteins, representing a COPI-independent ARF1 function. CRISPR/Cas9-edited ARF1; super-resolution nanoscopy (STED); dynamic confocal imaging; ARF1 GTP-hydrolysis mutant Molecular biology of the cell High 28428254
2018 BIG1/Arfgef1 and Arf1 regulate initiation of myelination by Schwann cells; Schwann cell-specific BIG1 knockout reduces myelin thickness and myelin protein zero membrane localization; BIG1 knockout decreases Arf1 binding to AP-1 clathrin adaptor subunits specifically, without affecting Arf1 binding to GGA1 or COPI. Conditional knockout mice (Schwann cell-specific BIG1 KO; Arf1 conditional KO); electron microscopy of myelin; co-immunoprecipitation of Arf1 with coat complexes Science advances High 29740613
2018 BIG2-ARF1 activates RhoA, which through mDia1 promotes Golgi deployment into major dendrites; BIG2 and ARF1 co-localize with the Golgi apparatus in hippocampal neurons; constitutively active ARF1(Q71L) rescues dendrite morphogenesis defects in BIG2-null neurons. siRNA knockdown; constitutively active ARF1 rescue; RhoA activation assay; immunofluorescence; in utero electroporation Molecular neurobiology Medium 29455446
2018 GBF1 and active Arf1-GTP interact with Miro (a mitochondrial membrane protein); inhibition of GBF1 or Arf1 activation promotes dynein- and Miro-dependent retrograde mitochondrial transport towards the centrosome; GBF1 inhibition results in a two-fold increase in retrograde mitochondrial movement. Co-immunoprecipitation of GBF1 and Arf1-GTP with Miro; live-cell mitochondrial tracking; GBF1 inhibition; Miro siRNA; dynein inhibitor Scientific reports Medium 30459446
2018 Cell-matrix adhesion controls Arf1 activation; loss of adhesion reduces active Arf1-GTP and disorganizes the Golgi along microtubules; constitutively active Arf1 prevents adhesion-dependent Golgi disorganization; adhesion-dependent Arf1 activation regulates Arf1 binding to dynein to control Golgi positioning and cell surface glycosylation. Co-immunoprecipitation (Arf1-dynein); constitutively active Arf1; integrin-blocking antibody; Arf1-GTP loading assay; surface glycosylation assay Journal of cell science Medium 30054383
2020 Arf1 ablation in cancer cells induces mitochondrial defects and ER stress, causing release of damage-associated molecular patterns (DAMPs) that recruit and activate dendritic cells; this triggers CD8+ T cell infiltration and activation, establishing Arf1-mediated lipid metabolism as a regulator of tumor immune surveillance. Arf1 genetic ablation; mitochondrial function assay; DAMP release assay; DC recruitment assay; T cell activation assay; mouse tumor models Nature communications Medium 31924786
2020 Low phosphatidylcholine (PC) synthesis or LPIN1 knockdown in mammalian cells reduces GTP-bound ARF1 levels, linking changes in lipid ratios (PC content) to ARF1 inactivation and consequent SREBP-1 maturation. RNAi screen in C. elegans; siRNA knockdown in mammalian cells; ARF1-GTP loading assay Cell reports Medium 27320911
2021 Arf1 directly recruits the Pik1-Frq1 PI4-kinase complex to the Golgi in yeast; this Arf1-dependent PI4P production is a critical upstream signal for AP-1 recruitment and secretory vesicle formation at maturing Golgi compartments. In vitro protein-protein interaction assay on Golgi-mimetic membranes; acute PI4P depletion; live-cell time-lapse imaging Molecular biology of the cell High 33788598
2021 Arf1 recruits Gyp1 (Rab-GAP) to the TGN to drive Ypt1 (Rab1) inactivation, thereby orchestrating Rab GTPase conversion on maturing Golgi compartments; Arf1 is a master regulator of Rab conversion through this GAP-recruitment mechanism. Yeast genetic analysis; live-cell imaging of Rab conversion; epistasis analysis with Arf1 and TRAPPII mutants Molecular biology of the cell Medium 33788577
2021 ARF1 interacts with IQGAP1 and promotes colon tumorigenesis via activation of ERK signaling and mitochondrial fission through enhanced IQGAP1-MEK-ERK interaction and increased Drp1 phosphorylation; the drug azelastine binds Thr-48 of ARF1 and inhibits this pathway. Co-immunoprecipitation; DARTS target identification; Biacore binding assay; ARF1-T48S mutant; cell proliferation and xenograft assays Theranostics Medium 33408784
2022 CryoEM structures of full-length Gea2 (yeast GBF1 ortholog) reveal organization of regulatory domains and how the GEF domain adopts two conformations corresponding to different stages of the Arf1 activation reaction; a Gea2-Arf1 activation intermediate structure suggests GEF domain movement primes Arf1 for membrane insertion upon GTP binding. CryoEM of full-length Gea2; structural analysis of Gea2-Arf1 intermediate Cell reports High 36044848
2023 Heterozygous GTPase-defective ARF1 missense mutations cause type I interferonopathy; mutated ARF1 perturbs mitochondrial morphology causing aberrant mitochondrial DNA release and cGAS activation, and also causes accumulation of active STING at the Golgi/ERGIC due to defective retrograde STING transport; ARF1 thus has a dual role in maintaining cGAS-STING homeostasis. Patient-derived cell lines with ARF1 missense mutations; IFN-stimulated gene expression assay; mitochondrial morphology analysis; STING trafficking assay; cell line overexpression of disease mutants Nature communications High 37914730
2023 A hyperactive Arf1 mutant in yeast decreases expression of fatty acid transporters and the rate-limiting β-oxidation enzyme, causing fatty acid accumulation in lipid droplets and mitochondrial fragmentation with reduced ATP synthesis; genetic/pharmacological depletion of fatty acids phenocopies the Arf1 mutant mitochondrial phenotype, linking Arf1 to fatty acid storage/utilization balance. Yeast hyperactive Arf1 mutant; transcriptomics; lipid droplet staining; mitochondrial morphology; ATP measurement; fatty acid depletion Nature cell biology High 37400497
2024 ARF1 compartments (tubulo-vesicular structures harboring clathrin and different AP complexes) comprise two functional classes: perinuclear ARF1 compartments facilitate Golgi export of secretory cargo, while peripheral ARF1 compartments mediate endocytic recycling downstream of early endosomes; ARF1 compartments mature into recycling endosomes, and this maturation requires AP-1. CRISPR-Cas9 endogenous tagging; fast confocal live-cell imaging; STED super-resolution microscopy; correlative light and electron microscopy; AP-1 depletion Nature cell biology High 39367144
2024 Astrocytic LRP1 suppresses lactate production and thereby reduces ARF1 lactylation; elevated ARF1 lactylation (a post-translational modification) in LRP1-depleted astrocytes impairs mitochondria transfer from astrocytes to neurons. LRP1 knockdown in astrocytes; ARF1 lactylation detection; mitochondria transfer assay; mouse ischemia-reperfusion model Cell metabolism Medium 38906140

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 ARF1, a transcription factor that binds to auxin response elements. Science (New York, N.Y.) 678 9188533
1995 Overexpression of wild-type and mutant ARF1 and ARF6: distinct perturbations of nonoverlapping membrane compartments. The Journal of cell biology 333 7896867
1995 The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization. Science (New York, N.Y.) 295 8533093
2014 Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting. eLife 245 24497546
2002 Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 208 12000962
2024 Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation. Cell metabolism 199 38906140
1997 The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1. The EMBO journal 150 9405360
2005 Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport. Nature cell biology 145 15821732
2008 Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proceedings of the National Academy of Sciences of the United States of America 144 18689681
2008 Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation. PLoS pathogens 130 18551169
2013 ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion. Molecular biology of the cell 118 24196838
1996 p619, a giant protein related to the chromosome condensation regulator RCC1, stimulates guanine nucleotide exchange on ARF1 and Rab proteins. The EMBO journal 117 8861955
2007 Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association. Molecular biology of the cell 115 17429068
2000 ARF1 regulates pH-dependent COP functions in the early endocytic pathway. The Journal of biological chemistry 113 10713138
2014 Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Molecular cell 104 25002144
2003 A role for Arf1 in mitotic Golgi disassembly, chromosome segregation, and cytokinesis. Proceedings of the National Academy of Sciences of the United States of America 104 14585930
2009 Structure and membrane interaction of myristoylated ARF1. Structure (London, England : 1993) 101 19141284
2006 A phosphatidylinositol-3-kinase-dependent signal transition regulates ARF1 and ARF6 during Fcgamma receptor-mediated phagocytosis. PLoS biology 100 16669702
2012 A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes. Cell 99 22304919
2020 Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nature communications 96 31924786
1994 An activating mutation in ARF1 stabilizes coatomer binding to Golgi membranes. The Journal of biological chemistry 96 8106346
2020 The U2AF2 /circRNA ARF1/miR-342-3p/ISL2 feedback loop regulates angiogenesis in glioma stem cells. Journal of experimental & clinical cancer research : CR 95 32894165
2007 Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes. The EMBO journal 90 17347647
2010 Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN. Nature cell biology 87 20228810
2002 ARF1 regulatory factors and COPI vesicle formation. Current opinion in cell biology 83 12383792
2001 DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. The Journal of biological chemistry 81 11773070
2009 Large Arf1 guanine nucleotide exchange factors: evolution, domain structure, and roles in membrane trafficking and human disease. Molecular genetics and genomics : MGG 78 19669794
2013 The small GTPase Arf1 modulates Arp2/3-mediated actin polymerization via PICK1 to regulate synaptic plasticity. Neuron 77 23889934
1995 Mutational analysis of Saccharomyces cerevisiae ARF1. The Journal of biological chemistry 77 7814365
1998 Building a secretory apparatus: role of ARF1/COPI in Golgi biogenesis and maintenance. Histochemistry and cell biology 76 9681627
2013 ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Molecular biology of the cell 73 23783033
2013 Recruitment of PI4KIIIβ to coxsackievirus B3 replication organelles is independent of ACBD3, GBF1, and Arf1. Journal of virology 65 24352456
2001 Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Molecular biology of the cell 65 11160834
2023 Arf1 coordinates fatty acid metabolism and mitochondrial homeostasis. Nature cell biology 63 37400497
2009 ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions. The EMBO journal 62 19927117
2005 Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling. The Journal of biological chemistry 62 15632162
2017 A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi. Molecular biology of the cell 60 28428254
2012 The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2. The Journal of cell biology 57 22291037
2012 ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell structure and function 56 22971977
2011 Interaction between the triglyceride lipase ATGL and the Arf1 activator GBF1. PloS one 54 21789191
2011 ARF1 controls proliferation of breast cancer cells by regulating the retinoblastoma protein. Oncogene 52 21478909
2007 p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking. The Journal of biological chemistry 50 17693410
2016 ARF1 promotes prostate tumorigenesis via targeting oncogenic MAPK signaling. Oncotarget 49 27213581
2021 Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission. Theranostics 48 33408784
2003 Selective interaction of ARF1 with the carboxy-terminal tail domain of the 5-HT2A receptor. Molecular pharmacology 47 14573774
2017 GBF1 and Arf1 function in vesicular trafficking, lipid homoeostasis and organelle dynamics. Biology of the cell 46 28985001
2010 Modifications to the C-terminus of Arf1 alter cell functions and protein interactions. Traffic (Copenhagen, Denmark) 45 20214751
2007 AP-1 and ARF1 control endosomal dynamics at sites of FcR mediated phagocytosis. Molecular biology of the cell 45 17914058
2006 Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking. Molecular biology of the cell 45 17182848
2003 The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. The Journal of biological chemistry 45 12771146
2016 Cholesterol-Independent SREBP-1 Maturation Is Linked to ARF1 Inactivation. Cell reports 44 27320911
2023 ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling. Nature communications 43 37914730
2014 ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis. Proceedings of the National Academy of Sciences of the United States of America 43 24707047
2021 Arf1 directly recruits the Pik1-Frq1 PI4K complex to regulate the final stages of Golgi maturation. Molecular biology of the cell 41 33788598
2021 Plumbagin suppresses non-small cell lung cancer progression through downregulating ARF1 and by elevating CD8+ T cells. Pharmacological research 41 33964470
2015 HIV-1 Nef hijacks clathrin coats by stabilizing AP-1:Arf1 polygons. Science (New York, N.Y.) 41 26494761
2014 The adaptor proteins p66Shc and Grb2 regulate the activation of the GTPases ARF1 and ARF6 in invasive breast cancer cells. The Journal of biological chemistry 41 24407288
2014 ARF1 regulates adhesion of MDA-MB-231 invasive breast cancer cells through formation of focal adhesions. Cellular signalling 41 25530216
2007 Degradation of the auxin response factor ARF1. The Plant journal : for cell and molecular biology 41 18088308
2005 Preparation of myristoylated Arf1 and Arf6. Methods in enzymology 41 16413267
2019 Histone deacetylase inhibitors suppress aggressiveness of head and neck squamous cell carcinoma via histone acetylation-independent blockade of the EGFR-Arf1 axis. Journal of experimental & clinical cancer research : CR 39 30777099
2004 ARF1 regulates Nef-induced CD4 degradation. Current biology : CB 39 15202998
2018 BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Science advances 38 29740613
2013 ARF1 controls Rac1 signaling to regulate migration of MDA-MB-231 invasive breast cancer cells. Cellular signalling 38 23707487
2015 Activation of Gαi at the Golgi by GIV/Girdin imposes finiteness in Arf1 signaling. Developmental cell 37 25865347
2004 ADRP is dissociated from lipid droplets by ARF1-dependent mechanism. Biochemical and biophysical research communications 37 15336557
2016 Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. The Journal of cell biology 36 28007915
2008 High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance. Molecular plant pathology 36 18705881
2007 ARF1-mediated actin polymerization produces movement of artificial vesicles. Proceedings of the National Academy of Sciences of the United States of America 34 17942688
2020 Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport. Molecular metabolism 33 32738348
2014 Interaction of Fapp1 with Arf1 and PI4P at a membrane surface: an example of coincidence detection. Structure (London, England : 1993) 33 24462251
2012 Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases. PloS one 33 22962618
2023 Arf1 Ablation in Colorectal Cancer Cells Activates a Super Signal Complex in DC to Enhance Anti-Tumor Immunity. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 31 37786300
2007 Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP. Traffic (Copenhagen, Denmark) 31 17451557
2012 Recruitment of Arf1-GDP to Golgi by Glo3p-type ArfGAPs is crucial for golgi maintenance and plant growth. Plant physiology 30 23266962
2021 Downregulation of long non-coding RNA LINC00460 inhibits the proliferation, migration and invasion, and promotes apoptosis of pancreatic cancer cells via modulation of the miR-320b/ARF1 axis. Bioengineered 29 33345740
2005 The role of ARF1 and rab GTPases in polarization of the Golgi stack. Traffic (Copenhagen, Denmark) 29 16101683
2003 Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ. American journal of physiology. Cell physiology 29 14684384
2015 The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo. Nature communications 28 25849195
2019 LncRNA TP73-AS1 is a novel regulator in cervical cancer via miR-329-3p/ARF1 axis. Journal of cellular biochemistry 27 31232491
2018 Activators and Effectors of the Small G Protein Arf1 in Regulation of Golgi Dynamics During the Cell Division Cycle. Frontiers in cell and developmental biology 27 29632863
2017 Overexpression of ARF1 is associated with cell proliferation and migration through PI3K signal pathway in ovarian cancer. Oncology reports 27 28098897
2017 Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics. Journal of experimental & clinical cancer research : CR 27 28830537
2014 Selective protection of an ARF1-GTP signaling axis by a bacterial scaffold induces bidirectional trafficking arrest. Cell reports 27 24582959
2002 Genetic interactions link ARF1, YPT31/32 and TRS130. Yeast (Chichester, England) 27 12210902
2018 Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling. PloS one 26 29614107
2018 Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells. Journal of cell science 26 30054383
2018 GBF1 and Arf1 interact with Miro and regulate mitochondrial positioning within cells. Scientific reports 26 30459446
2020 Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition. Nucleic acids research 25 31875226
2005 Membrane curvature and the control of GTP hydrolysis in Arf1 during COPI vesicle formation. Biochemical Society transactions 25 16042557
2024 Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex. Cell metabolism 24 39442522
2009 Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Proceedings of the National Academy of Sciences of the United States of America 24 19332778
2018 BIG2-ARF1-RhoA-mDia1 Signaling Regulates Dendritic Golgi Polarization in Hippocampal Neurons. Molecular neurobiology 23 29455446
2018 Arf1 regulates the ER-mitochondria encounter structure (ERMES) in a reactive oxygen species-dependent manner. The FEBS journal 23 29603662
2005 Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1. Current biology : CB 23 16332543
1994 ARF1(2-17) does not specifically interact with ARF1-dependent pathways. Inhibition by peptide of phospholipases C beta, D and exocytosis in HL60 cells. FEBS letters 23 8045298
2024 ARF1 compartments direct cargo flow via maturation into recycling endosomes. Nature cell biology 22 39367144
2022 Profiling of phytohormone-specific microRNAs and characterization of the miR160-ARF1 module involved in glandular trichome development and artemisinin biosynthesis in Artemisia annua. Plant biotechnology journal 22 36478140
2021 Arf1 orchestrates Rab GTPase conversion at the trans-Golgi network. Molecular biology of the cell 22 33788577
2022 Structural basis for activation of Arf1 at the Golgi complex. Cell reports 21 36044848

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