Affinage

PLEKHG5

Pleckstrin homology domain-containing family G member 5 · UniProt O94827

Length
1006 aa
Mass
111.2 kDa
Annotated
2026-06-10
90 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PLEKHG5 (Syx/Tech) is a DH-PH domain guanine nucleotide exchange factor that selectively activates RhoA to control actomyosin organization, cell polarity, junction integrity, and migration across neuronal, endothelial, epithelial, and tumor contexts (PMID:15686487, PMID:18824598, PMID:23253477). Its output is spatially confined through scaffolded complexes: a C-terminal PDZ-binding motif tethers it to Amot–Patj/MUPP1 to position RhoA activity at endothelial lamellipodia (PMID:18824598, PMID:18537874), and Crumbs-complex recruitment to cell junctions drives integrity via the RhoA effector Diaphanous/Dia1 (PMID:23253477, PMID:24126053). This activity is switched off by phosphorylation-dependent inhibition: VEGF/PKD1-mediated phosphorylation (Ser806, Ser92, Ser938) couples PLEKHG5 to 14-3-3 proteins, removing it from junctions and suppressing GEF activity, whereas phospho-deficient PLEKHG5 shows enhanced junctional targeting and actin-ring strengthening (PMID:23253477, PMID:23335514); binding of Rnd3 to a Raf1-like RBD provides an additional layer of negative regulation independent of the DH domain (PMID:20811643). Membrane and apical targeting occur through the PH domain, which also mediates self-association required for GEF activity and allosterically regulates the DH domain against RhoA (PMID:35639414, PMID:39196644). In motor neurons PLEKHG5 also acts as a GEF for Rab26 to direct autophagy of synaptic vesicles and unconventional secretion of Sod1 via lysosome-related organelles, and its loss causes late-onset motor neuron degeneration and peripheral nerve pathology in mice (PMID:29084947, PMID:39366938, PMID:32733205). PLEKHG5 mutations cause inherited motor neuron disease and peripheral neuropathy, with disease alleles impairing protein stability, localization, NF-κB signaling, and PH-domain self-association (PMID:17564964, PMID:39196644). Recurrently across cancers, NRP1/GIPC1- and VEGF-A-driven PLEKHG5–RhoA signaling promotes proliferation, invasion, and cell-cycle progression (PMID:26209534, PMID:30456845, PMID:37427593).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2005 High

    Establishing PLEKHG5 as a RhoA-specific GEF defined its core biochemical activity and linked it to neuronal morphology.

    Evidence In vitro GEF assay across Rho subfamily members with DH-domain point mutagenesis and dominant-negative RhoA in cortical neurons

    PMID:15686487

    Open questions at the time
    • Did not address regulation of GEF activity
    • No endogenous localization or partners defined
  2. 2007 Medium

    Linking PLEKHG5 to NF-κB activation and to a disease-causing missense mutation tied its molecular activity to motor neuron disease.

    Evidence Wild-type vs p.Phe647Ser mutant transfection in HEK293/MCF10A/NSC34 with NF-κB reporters, localization, and aggregate detection

    PMID:17564964

    Open questions at the time
    • Mechanism connecting GEF activity to NF-κB not resolved
    • Aggregation cause vs consequence unclear
  3. 2008 High

    Identification of Amot–Patj/MUPP1 scaffolds and a role in angiogenic sprouting explained how RhoA activity is spatially confined and gave PLEKHG5 a developmental vascular function.

    Evidence Y2H, peptide pull-down, FRET of RhoA in lamellipodia, endogenous Co-IP from brain, and zebrafish morpholino knockdown with rescue

    PMID:18537874 PMID:18757825 PMID:18824598

    Open questions at the time
    • How recruitment is dynamically regulated not yet shown
    • Effector downstream of localized RhoA not identified at this stage
  4. 2010 High

    Discovery of Rnd3 binding to a Raf1-like RBD revealed DH-independent negative regulation of PLEKHG5 GEF activity in vivo.

    Evidence Affinity purification/MS, RBD point mutagenesis (E164A/R165D), and zebrafish axis rescue assays

    PMID:20811643

    Open questions at the time
    • Structural basis of RBD-mediated inhibition not defined
    • Physiological signals controlling Rnd3 binding unknown
  5. 2013 High

    Phosphoregulation via PKD1/14-3-3 and selective Dia1 (not ROCK) engagement defined a complete on/off switch and effector branch governing junction stability and directional migration.

    Evidence Co-IP, in vitro kinase and GEF assays, phosphomutant constructs, junctional actin imaging, and siRNA migration assays in MDCK and tumor cells

    PMID:23335514 PMID:24126053

    Open questions at the time
    • Stoichiometry and kinetics of multi-site phosphorylation not resolved
    • How Dia1 is selected over ROCK mechanistically unclear
  6. 2012 High

    A syx knockout mouse demonstrated that junctional RhoA-Diaphanous signaling controls vascular barrier integrity in vivo, with VEGF/PKD1 and Ang1 setting junction disassembly versus stability.

    Evidence syx-/- mouse with vascular permeability and cardiac function assays, PKD1 kinase assay, Ser806 phosphomutants, and junctional fractionation

    PMID:23253477

    Open questions at the time
    • Cell-type-specific contributions in vivo not dissected
    • Link between cardiac phenotype and direct PLEKHG5 function incomplete
  7. 2017 High

    Identification of PLEKHG5 as a Rab26 GEF redefined it as a regulator of synaptic-vesicle autophagy whose loss causes motor neuron degeneration, expanding its substrate range beyond RhoA.

    Evidence Plekhg5 knockout mouse, cultured motoneuron knockdown, Rab26 GEF assay, autophagy reporters, and constitutively active Rab26 rescue

    PMID:29084947

    Open questions at the time
    • Determinants of RhoA vs Rab26 substrate choice unknown
    • Spatial control of Rab26 activation in axon terminals not resolved
  8. 2018 High

    PH-domain-dependent apical cortex targeting coupling GEF activity to apical actomyosin established PLEKHG5 as a driver of apical constriction during gastrulation.

    Evidence Xenopus morpholino knockdown, GEF-dead mutants, PH-domain truncations, actomyosin imaging, and Rho inhibitor tests

    PMID:30446627

    Open questions at the time
    • PH-domain lipid/membrane ligand not identified at this stage
    • How GEF activity feeds back on apical recruitment mechanistically unclear
  9. 2015 Medium

    NRP1/GIPC1 complex formation showed PLEKHG5 functions downstream of VEGF-A receptors to drive RhoA-dependent proliferation and invasion in cancer.

    Evidence Co-IP, siRNA knockdown, RhoA-GTP pull-down, proliferation/spheroid/invasion assays, and constitutively active RhoA/p38 rescue in skin cancer and ECS cells

    PMID:26209534 PMID:30456845

    Open questions at the time
    • Direct vs indirect NRP1 binding not fully resolved
    • Whether GIPC1 alters localization or activity is unclear
  10. 2020 Medium

    Knockout phenotypes in glioblastoma and peripheral nerve linked PLEKHG5 loss to defective autophagy, reduced RhoA, myelin pathology, and altered immune signature, broadening its physiological footprint.

    Evidence CRISPR knockout with autophagy reporters and RAB26QL rescue in U251 cells; Plekhg5 knockout mouse with nerve EM, T-cell profiling, and RNAseq

    PMID:32733205 PMID:33318498

    Open questions at the time
    • Mechanism connecting RhoA and Rab26 outputs to autophagy not unified
    • Cell-autonomous vs non-autonomous immune effects undefined
  11. 2022 Medium

    Biophysical and computational characterization of purified PLEKHG5 supported PH-domain allosteric regulation of DH-domain GEF activity toward membrane-embedded RhoA.

    Evidence Recombinant expression, circular dichroism, size-exclusion co-elution with RhoA, homology modeling, and molecular dynamics on a realistic membrane

    PMID:35639414

    Open questions at the time
    • Allosteric site not validated by mutagenesis
    • No experimental structure of the full protein
  12. 2024 High

    PH-domain self-association required for GEF activity and apical localization, with a disease mutation abolishing it, mechanistically connected a structural feature to function and pathology.

    Evidence Xenopus deletion/point mutants, self-association Co-IP, in trans rescue, and apical constriction readouts

    PMID:39196644 PMID:39366938

    Open questions at the time
    • Oligomeric stoichiometry not defined
    • Whether self-association is regulated by signaling unknown
  13. 2025 Low

    An RND3–PLEKHG5 axis was reported to modulate autophagy and oxidative stress in endometriosis, extending Rnd3 regulation to a new disease context.

    Evidence Co-IP, siRNA knockdown, autophagy and oxidative stress markers, and a mouse EMS model

    PMID:41137701

    Open questions at the time
    • Single Co-IP without reciprocal structural mapping in this context
    • Reported transcriptional upregulation of PLEKHG5 by RND3 mechanism unexplained
    • Direct vs indirect effects on autophagy not separated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PLEKHG5 selects between its RhoA and Rab26 substrates, and how its scaffolds, phosphorylation, and PH-domain self-association are integrated to choose between junction control, migration, apical constriction, and synaptic-vesicle autophagy in a given cell, remains unresolved.
  • No unified model of RhoA vs Rab26 substrate switching
  • No full-length experimental structure
  • Context-specific regulatory inputs incompletely mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Localization
GO:0005886 plasma membrane 5 GO:0005856 cytoskeleton 3
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1266738 Developmental Biology 3 R-HSA-9612973 Autophagy 3
Partners
AMOTGIPC1MPDZNRP1RAB26RHOARND3YWHAB
Complex memberships
Amot–Patj/MUPP1 ternary complexNRP1–GIPC1–Syx complex

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 Tech (PLEKHG5) selectively binds to and activates RhoA (but not Rac1 or Cdc42) via its DH domain in vitro, and a constitutively active Tech construct decreases dendritic process number in cortical neurons; this effect is blocked by a DH-domain point mutation abolishing RhoA activation or by dominant-negative RhoA. In vitro GEF assay with prototypical Rho subfamily members; point mutagenesis of DH domain; dominant-negative RhoA co-expression; primary cortical neuron morphology readout Journal of neurochemistry High 15686487
2007 Wild-type PLEKHG5 activates the NF-κB signaling pathway in transfected HEK293 and MCF10A cells; a disease-causing missense mutation (p.Phe647Ser) alters protein stability and intracellular localization, severely impairing NF-κB transduction and causing protein aggregates in NSC34 motor neurons. Transient transfection of wild-type and mutant PLEKHG5 in HEK293, MCF10A, and NSC34 cells; NF-κB reporter assays; immunofluorescence localization; aggregate detection American journal of human genetics Medium 17564964
2008 Syx (PLEKHG5) forms a ternary complex with Amot and Patj/Mupp1 via its C-terminal PDZ-binding motif; this complex spatially controls RhoA GTPase activity at lamellipodia of migrating endothelial cells as shown by FRET analysis. Peptide pull-down, yeast two-hybrid screening, FRET analysis of RhoA activity in lamellipodia, morpholino knockdown in zebrafish Blood High 18824598
2008 Syx (PLEKHG5) is required for angiogenic sprouting in vivo; morpholino knockdown in zebrafish specifically impairs vascular sprouting without affecting vasculogenesis or angioblast differentiation, and this defect is partially rescued by mouse Syx mRNA; Syx knockdown in vitro impairs VEGF-A-induced endothelial cell migration. Morpholino knockdown in zebrafish; mRNA rescue; in vitro endothelial migration assay Circulation research High 18757825
2008 Tech (PLEKHG5) binds to MUPP1 PDZ domains 10 and 13 via its C-terminal PDZ ligand motif; endogenous Tech co-precipitates with MUPP1 (but not PSD-95) from hippocampal/cortical extracts, and the two proteins co-localize at peri-synaptic puncta in cortical neurons. Yeast two-hybrid; co-transfection/co-IP in HEK293; endogenous co-IP from rat brain extracts; PDZ-domain mutagenesis; immunofluorescence co-localization Journal of neurochemistry High 18537874
2010 Rnd3 (RhoE) directly interacts with Syx (PLEKHG5) via a Raf1-like Ras-binding domain (RBD) in Syx, identified by affinity purification/mass spectrometry; this interaction does not involve the Syx DH domain. A Rnd3-binding-defective Syx mutant (E164A/R165D) is more potent in rescuing zebrafish axis defects than wild-type, indicating Rnd3 negatively regulates Syx GEF activity in vivo. Two-step affinity purification/mass spectrometry; co-IP; RBD point mutagenesis; zebrafish morpholino knockdown and mRNA rescue PloS one High 20811643
2012 Syx (PLEKHG5) is recruited to endothelial cell junctions by members of the Crumbs polarity complex and promotes junction integrity by activating the RhoA downstream effector Diaphanous; VEGF causes PKD1-mediated phosphorylation of Syx at Ser806, reducing its association with junctional anchors and promoting junction disassembly; Ang1 maintains Syx at junctions to stabilize them. syx−/− mice display defective junctions, vascular leakiness, edema, and impaired heart function. Co-IP; subcellular fractionation/localization; PKD1 kinase assay; phosphomutant constructs; syx knockout mouse; vascular permeability and cardiac function assays The Journal of cell biology High 23253477
2013 14-3-3 proteins interact with Syx (PLEKHG5) at both N- and C-terminal regions in a phosphorylation-dependent manner; PKD-mediated phosphorylation at Ser92 and additional phosphorylation at Ser938 are critical for 14-3-3 association. 14-3-3 binding inhibits Syx GEF activity; phosphorylation-deficient, 14-3-3-uncoupled Syx shows increased junctional targeting and GEF activity, strengthening the circumferential junctional actin ring in MDCK cells. Co-IP; in vitro kinase assay; phosphomutant constructs; GEF activity assay; immunofluorescence of junctional actin The Journal of biological chemistry High 23335514
2013 Syx (PLEKHG5) is required for polarity of migrating brain and breast tumor cells; this function is mediated by selective activation of the RhoA effector Dia1, leading to microtubule reorganization, downregulation of focal adhesions and actin stress fibers, and activation of cofilin-mediated actin reorganization. Syx recruitment to the membrane suppresses ROCK activity while activating Dia1. siRNA knockdown; live-cell migration assay; Dia1 and ROCK activity measurements; microtubule and focal adhesion immunofluorescence Molecular and cellular biology Medium 24126053
2015 VEGF-A/NRP1 signaling induces formation of a GIPC1–Syx (PLEKHG5) complex; this complex activates RhoA and promotes cell proliferation in DJM-1 skin cancer cells. Knockdown of GIPC1 or Syx reduces active RhoA and proliferation; constitutively active RhoA rescues proliferation in siVEGF-A cells. A cell-penetrating oligopeptide targeting GIPC1/Syx complex formation inhibits RhoA activation. Co-IP; siRNA knockdown; RhoA-GTP pull-down; proliferation assays; constitutively active RhoA rescue; inhibitory peptide Biology open Medium 26209534
2017 Plekhg5 acts as a guanine exchange factor for Rab26, a small GTPase that directs synaptic vesicles to preautophagosomal structures; Plekhg5 gene knockout in mice causes late-onset motor neuron disease with degeneration of axon terminals. Cultured Plekhg5-depleted motoneurons show defective axon growth and impaired autophagy of synaptic vesicles, rescued by constitutively active Rab26. Plekhg5 knockout mouse; cultured motoneuron knockdown; GEF assay for Rab26; autophagy reporters; axon growth measurements; constitutively active Rab26 rescue Nature communications High 29084947
2017 Plekhg5 (PLEKHG5) regulates cell polarity, directional migration, adhesion, podosome organization, and bone resorption in macrophages and osteoclasts; depletion causes abnormal localization of mDia1, LIMK1, cofilin, EB1, and vinculin, with upregulation of mDia1 and LIMK1 protein levels. siRNA knockdown in macrophages and osteoclasts; migration assay; podosome and bone resorption assays; immunofluorescence of Rho effectors Experimental cell research Medium 28847484
2018 Plekhg5 RhoGEF activity is required for apical constriction of bottle cells during Xenopus gastrulation; Plekhg5 protein localizes to the apical cell cortex via its pleckstrin homology (PH) domain, and GEF activity enhances this apical recruitment. Plekhg5 induces apical actomyosin accumulation and cell elongation; knockdown inhibits activin-induced bottle cell formation and blastopore lip formation in a Rho-dependent manner. Morpholino knockdown in Xenopus embryos; GEF-activity-deficient mutants; PH domain truncations; F-actin/myosin immunofluorescence; ectopic bottle cell induction; Rho inhibitor treatment Development (Cambridge, England) High 30446627
2018 NRP-1 forms a complex with GIPC1 and Syx (PLEKHG5) to activate RhoA/ROCK-dependent p38 MAPK activity, enhancing epidermal cancer stem cell (ECS) spheroid formation, invasion, migration, and angiogenic potential; constitutively active RhoA or p38 in NRP1-knockout cells restores the ECS cell phenotype. Co-IP; siRNA/shRNA knockdown; RhoA-GTP pull-down; p38 activity assay; spheroid and invasion assays; constitutively active rescue Molecular carcinogenesis Medium 30456845
2020 PLEKHG5 knockout in U251-MG glioblastoma cells (via CRISPR/Cas9) impairs autophagy (accumulation of autolysosomes, decreased LAMP-1), reduces RhoA activity, alters morphology, and reduces filopodia; rescue by constitutively active RAB26 (RAB26QL) restores RhoA levels, autophagy, and cellular fitness, and RAB26 overexpression activates MGMT expression. CRISPR/Cas9 knockout; mRFP-GFP-LC3 autophagy reporter; RAB26QL lentiviral rescue; RhoA activity assay; LAMP-1 immunofluorescence Scientific reports Medium 33318498
2020 Absence of Plekhg5 in mice results in myelin infoldings in peripheral nerves and impaired Schwann cell autophagy, with a reduced number of CD4+ and CD8+ T-cells in sciatic nerves; RNAseq identified a transcriptional signature of impaired immune response in Plekhg5-deficient peripheral nerves. Plekhg5 knockout mouse; electron microscopy of peripheral nerves; T-cell immunofluorescence/flow cytometry; RNAseq of sciatic nerves Frontiers in cellular neuroscience Medium 32733205
2022 Human Syx (PLEKHG5) can be expressed, purified, and shown to be folded by circular dichroism; it actively binds RhoA as determined by co-elution in size exclusion chromatography. Molecular dynamics simulations on a physiologically realistic membrane reveal novel allosteric interactions between the PH domain and the membrane-embedded region of RhoA, supporting PH domain allosteric regulation of DH-domain GEF activity. Recombinant protein expression/purification; circular dichroism spectroscopy; size exclusion chromatography co-elution; homology modeling; molecular dynamics simulation FASEB journal Medium 35639414
2023 The Syx–RhoA–Dia1 signaling axis promotes GBM cell cycle progression; Syx depletion causes prolonged mitosis, increased DNA damage, G2/M arrest, and apoptosis. These effects are phenocopied by Dia1 depletion and are mediated, at least in part, by increased phosphorylation, cytoplasmic retention, and reduced activity of YAP/TAZ transcriptional coactivators. Targeting Syx cooperates with radiation and temozolomide to decrease GBM cell viability. siRNA knockdown; orthotopic GBM xenografts; cell cycle analysis; DNA damage markers; YAP/TAZ phosphorylation and localization; combination drug/radiation assays JCI insight Medium 37427593
2023 HDAC2 directly interacts with PLEKHG5 and deacetylates lysine residues within its PH domain, maintaining PLEKHG5 protein stability. HDAC2 knockout or selective inhibition reduces PLEKHG5 protein levels and sensitizes HCC cells to sorafenib; overexpression of PLEKHG5 in HDAC2-KO cells restores sorafenib resistance. PLEKHG5 overexpression activates Rac1/AKT/NF-κB signaling. Co-IP; HDAC2 KO; selective HDAC2 inhibitor; ubiquitination/acetylation assays; in vitro and in vivo HCC drug sensitivity assays Cell death discovery Medium 37248230
2023 Plekhg5 regulates both medioapical and junctional actomyosin dynamics during apical constriction of Xenopus bottle cells; knockdown of plekhg5 disrupts medioapical and junctional actomyosin activity and apical constriction without affecting initial F-actin dynamics. Correlation of apical constriction with medioapical actomyosin localization is stronger than with junctional actomyosin. Morpholino knockdown in Xenopus embryos; live imaging; quantitative image analysis of actomyosin signals; F-actin dynamics measurements Molecular biology of the cell Medium 37043306
2024 Plekhg5 apical cortex localization requires N-terminal sequences and intact GEF activity; C-terminal sequences prevent basolateral mis-localization. Plekhg5 self-associates via its PH domain, and this self-association functionally rescues in trans two mutants lacking the N-terminal region or GEF activity. A disease-associated PH domain point mutation abolishes self-association and fails to induce apical constriction. Deletion and point mutant constructs in Xenopus; subcellular localization imaging; co-IP for self-association; in trans rescue assay; apical constriction functional readout Molecular biology of the cell High 39196644
2024 Plekhg5 drives unconventional secretion (UPS) of Sod1 by sequestering Sod1 into autophagosomal carriers that fuse with secretory lysosome-related organelles (LROs); exocytosis of LROs requires Plekhg5-mediated activation of Rab26. Plekhg5 deletion in mice causes Sod1 accumulation in LROs at swollen presynaptic sites. In SOD1-G93A/Plekhg5-deleted mice, reduced secretion of toxic SOD1 accelerated disease onset but prolonged survival via attenuated microglial activation. Human iPSC-derived motoneurons with reduced PLEKHG5 show impaired ALS-linked SOD1 secretion. Plekhg5 knockout mouse; SOD1G93A/Plekhg5 double-mutant mouse; autophagosome/LRO marker co-localization; Rab26 GEF assay; secretion assay; iPSC-derived motoneurons; microglial activation markers Nature communications High 39366938
2025 RND3 directly interacts with PLEKHG5 (shown by Co-IP) and upregulates PLEKHG5 expression; RND3 overexpression in ectopic endometrial stromal cells enhances autophagy and suppresses oxidative stress in a PLEKHG5-dependent manner, inhibiting EMS progression in vitro and in vivo. Co-IP; siRNA knockdown; autophagy markers; oxidative stress assays; mouse EMS model FASEB journal Low 41137701

Source papers

Stage 0 corpus · 90 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells. Blood 115 18824598
2007 Nutritional recommendations of feedlot consulting nutritionists: the 2007 Texas Tech University survey. Journal of animal science 114 17591710
2007 The nuclear factor kappaB-activator gene PLEKHG5 is mutated in a form of autosomal recessive lower motor neuron disease with childhood onset. American journal of human genetics 77 17564964
2012 VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx. The Journal of cell biology 74 23253477
2017 Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nature communications 66 29084947
2002 Tech.Sight. Gene therapy. Hurdles and hopes for cancer treatment. Science (New York, N.Y.) 66 12130788
2019 TECH-VER: A Verification Checklist to Reduce Errors in Models and Improve Their Credibility. PharmacoEconomics 60 31705406
2014 RNA Interference (RNAi) Induced Gene Silencing: A Promising Approach of Hi-Tech Plant Breeding. International journal of biological sciences 58 25332689
2002 Tech.Sight. Phage display. Affinity selection from biological libraries. Science (New York, N.Y.) 57 12386335
2008 Syx, a RhoA guanine exchange factor, is essential for angiogenesis in Vivo. Circulation research 54 18757825
2017 Concise Review: The High Cost of High Tech Medicine: Planning Ahead for Market Access. Stem cells translational medicine 40 28749065
2019 New High-Tech Flexible Networks for the Monitoring of Deep-Sea Ecosystems. Environmental science & technology 39 31074981
2005 Tech: a RhoA GEF selectively expressed in hippocampal and cortical neurons. Journal of neurochemistry 36 15686487
2012 Marker-trait associations in Virginia Tech winter barley identified using genome-wide mapping. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 31 23139143
2008 The neuronal RhoA GEF, Tech, interacts with the synaptic multi-PDZ-domain-containing protein, MUPP1. Journal of neurochemistry 30 18537874
2015 VEGF-A/NRP1 stimulates GIPC1 and Syx complex formation to promote RhoA activation and proliferation in skin cancer cells. Biology open 29 26209534
2013 PLEKHG5 deficiency leads to an intermediate form of autosomal-recessive Charcot-Marie-Tooth disease. Human molecular genetics 29 23777631
2013 Mutations in the PLEKHG5 gene is relevant with autosomal recessive intermediate Charcot-Marie-Tooth disease. Orphanet journal of rare diseases 29 23844677
2010 The RhoA GEF Syx is a target of Rnd3 and regulated via a Raf1-like ubiquitin-related domain. PloS one 27 20811643
2018 The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation. Development (Cambridge, England) 26 30446627
2021 Highly comparable metabarcoding results from MGI-Tech and Illumina sequencing platforms. PeerJ 25 34703674
2013 The Rho guanine nucleotide exchange factor Syx regulates the balance of dia and ROCK activities to promote polarized-cancer-cell migration. Molecular and cellular biology 24 24126053
2018 NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival. Molecular carcinogenesis 21 30456845
2022 A low-tech, cost-effective and efficient method for safeguarding genetic diversity by direct cryopreservation of poultry embryonic reproductive cells. eLife 20 35074046
2007 A low-cost and low-tech electrochemical flow system for the evaluation of total phenolic content and antioxidant power of tea infusions. Talanta 18 18371884
2024 Plekhg5 controls the unconventional secretion of Sod1 by presynaptic secretory autophagy. Nature communications 17 39366938
2013 Phosphorylation-mediated 14-3-3 protein binding regulates the function of the rho-specific guanine nucleotide exchange factor (RhoGEF) Syx. The Journal of biological chemistry 14 23335514
2017 The Rho-specific guanine nucleotide exchange factor Plekhg5 modulates cell polarity, adhesion, migration, and podosome organization in macrophages and osteoclasts. Experimental cell research 13 28847484
1999 Low-tech electrophoresis, small but beautiful, and effective: electrophoretic titration curves of proteins. Electrophoresis 12 10424454
1997 Caval incorporation of the LGM Vena Tech filter: an experimental study. Journal of vascular and interventional radiology : JVIR 12 9152915
2023 PLEKHG5 is stabilized by HDAC2-related deacetylation and confers sorafenib resistance in hepatocellular carcinoma. Cell death discovery 11 37248230
2020 PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells. Scientific reports 11 33318498
2008 Prenatal diagnosis of beta-thalassemia in Egypt: implementing accurate high-tech methods did not reflect much on the outcome. Pediatric hematology and oncology 11 18728973
2022 The adverse effects of synthetic acaricide tau-fluvalinate (tech.) on winter adult honey bees. Environmental toxicology and pharmacology 10 35398274
2020 LAMP-LFD Based on Isothermal Amplification of Multicopy Gene ORF160b: Applicability for Highly Sensitive Low-Tech Screening of Allergenic Soybean (Glycine max) in Food. Foods (Basel, Switzerland) 10 33255927
2016 Low-Tech, Pilot Scale Purification of a Recombinant Spider Silk Protein Analog from Tobacco Leaves. International journal of molecular sciences 10 27735843
2023 High-Tech Methods of Cytokine Imbalance Correction in Intervertebral Disc Degeneration. International journal of molecular sciences 9 37686139
2017 Fabrication of paper devices via laser-heating-wax-printing for high-tech enzyme-linked immunosorbent assays with low-tech pen-type pH meter readout. The Analyst 9 28106171
2023 The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction during Xenopus gastrulation. Molecular biology of the cell 8 37043306
2023 A Syx-RhoA-Dia1 signaling axis regulates cell cycle progression, DNA damage, and therapy resistance in glioblastoma. JCI insight 8 37427593
2020 Novel variants broaden the phenotypic spectrum of PLEKHG5-associated neuropathies. European journal of neurology 7 33220101
2003 Modeling regulatory networks at Virginia Tech. Omics : a journal of integrative biology 7 14583117
2023 Effects of prolonged stimulation with heated tobacco products (Ploom TECH+ ) on gingival epithelial cells. Journal of periodontal research 6 36974375
2020 Absence of Plekhg5 Results in Myelin Infoldings Corresponding to an Impaired Schwann Cell Autophagy, and a Reduced T-Cell Infiltration Into Peripheral Nerves. Frontiers in cellular neuroscience 6 32733205
2010 Any use in proteomics for low-tech approaches? Detecting fibrinogen chains of different animal species in two-dimensional electrophoresis patterns. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 6 20674523
2023 The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing. Cell reports 5 37910502
2022 Sperm selection with Annexin-V coated polystrene bead technique (APB-Tech): A novel and reliable method for the microscopic selection of viable and non-apoptotic sperm to be used for intracytoplasmic sperm injection. Theriogenology 5 36209549
2021 Leukoencephalopathy and conduction blocks in PLEKHG5-associated intermediate CMT disease. Neuromuscular disorders : NMD 5 34244018
2014 Medium-term outcome of Astra Tech implants in head and neck oncology patients. International journal of oral and maxillofacial surgery 5 24907130
2012 An Innovative High-Tech Acupuncture Product: SXDZ-100 Nerve Muscle Stimulator, Its Theoretical Basis, Design, and Application. Evidence-based complementary and alternative medicine : eCAM 5 22454672
2021 PLEKHG5-related autosomal recessive lower motor neuron disease with dysmyelination in peripheral nerves. Clinical neuropathology 4 34236308
2020 A Pilot Comparison of High- Versus Low-Tech Palatability Assessment Tools in Young Children. Paediatric drugs 4 33236188
2018 Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms. Journal of visualized experiments : JoVE 4 30035770
2017 Dietary Self-management in Heart Failure: High Tech or High Touch? Current treatment options in cardiovascular medicine 4 28299612
2024 The RhoGEF protein Plekhg5 self-associates via its PH domain to regulate apical cell constriction. Molecular biology of the cell 3 39196644
2023 A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers During Drug Treatments. Methods in molecular biology (Clifton, N.J.) 3 36715910
2020 High-Tech and Nature-Made Nanocomposites and Their Applications in the Field of Sensors and Biosensors for Gas Detection. Biosensors 3 33203038
2020 Novel PLEKHG5 mutations in a patient with childhood-onset lower motor neuron disease. Annals of clinical and translational neurology 3 33275839
2015 Short communication: Comparison of predicted dietary phosphorus balance using bioavailabilities from the NRC (2001) and Virginia Tech model. Journal of dairy science 3 26709165
2007 [Doping. High-tech cheating in sport]. Der Internist 3 17426943
2005 [The peptide nucleic acids (PNAs): "high-tech" probes for genetic and molecular cytogenetic investigations]. Medecine sciences : M/S 3 16115462
2003 Structural biology: a high-tech tool for biomedical research. Current opinion in nephrology and hypertension 3 12815340
2025 CDH-3/cadherin, YAP-1/YAP, and EGL-44/TEAD promote SYX-2/syntaxin and EFF-1 fusogen-mediated phagosome closure. Genetics 2 40898783
2024 Diagnostic utility of Cyttel-Tech in identifying meningeal metastases from malignant solid tumors: An observational study. Medicine 2 39560579
2022 Characterization and computational simulation of human Syx, a RhoGEF implicated in glioblastoma. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2 35639414
2019 The tech for the next decade: promises and challenges in genome biology. Genome biology 2 31039798
2007 Virginia Tech disaster response shows value of regular drills and planning. ED management : the monthly update on emergency department management 2 17628964
2025 Wearable Arduino-Based Electronic Interactive Tattoo: A New Type of High-Tech Humanized Emotional Expression for Electronic Skin. Sensors (Basel, Switzerland) 1 40218664
2025 Validation of single-cell transcriptomic profiles from Illumina and MGI Tech sequencing platforms. FEBS letters 1 40490970
2024 Molecular epidemiological analysis of Influenza viruses in Influenza-like illness cases: a retrospective study in Chongqing Hi-Tech Zone, China (2021-2024). Virology journal 1 39741357
2023 A comparative study between low- and high-tech methods for the detection and mitigation of illicit connections in stormwater systems. Water science and technology : a journal of the International Association on Water Pollution Research 1 37830999
2022 Homozygous N-terminal missense variant in PLEKHG5 associated with intermediate CMT: A case report. Journal of neuromuscular diseases 1 34897098
2022 A Simple and Low-Tech Heat-Shock Method to Increase Genome Editing Efficiency in Plants. Current protocols 1 36469612
2010 High-tech/high-touch team-centered care provides best outcomes for wound prevention in critically ill patients. Critical care nursing quarterly 1 20827064
1999 A plethora of hi-tech vaccines -- genetic, edible, sugar glass, and more. CVI forum 1 12349328
1989 Regulatory affairs and biotechnology in Europe. II. The CPMP, "High Tech" and Multi-State procedures. Biotherapy (Dordrecht, Netherlands) 1 2701848
2026 Gene Flooding: Proposal to Flood Invasive Populations With Inbred Individuals as a Form of Low-Tech Genetic Control. Ecology and evolution 0 41568019
2026 RNA interference tech takes aim at poultry mites. The Veterinary record 0 41615019
2026 Coaching Leadership and Employees' Bootlegging Innovation Behavior in Chinese High-Tech Enterprises. Behavioral sciences (Basel, Switzerland) 0 42073847
2025 CDH-3/Cadherin, YAP-1/YAP and EGL-44/TEAD promote SYX-2/Syntaxin and EFF-1 fusogen-mediated phagosome closure. bioRxiv : the preprint server for biology 0 40236144
2025 Melanoma 3.0T-Tech Innovations, New Targeted Therapies, and T-Cell Breakthroughs. American Society of Clinical Oncology educational book. American Society of Clinical Oncology. Annual Meeting 0 40632948
2025 Catalyzing clean tech Innovation: The role of intellectual property protection in China's new energy sector. Journal of environmental management 0 40680623
2025 Tech strain: the musculoskeletal impact of electronic devices on young adults: A cross-sectional study. JPMA. The Journal of the Pakistan Medical Association 0 40751618
2025 RND3 Inhibits Endometriosis Progression by Regulating Autophagy and Oxidative Stress Through PLEKHG5. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 41137701
2024 Comparative analysis of the toxic effects on the mouse lung of 4 weeks exposure to the heated tobacco product Ploom TECH+ and 3R4F reference cigarettes. Journal of toxicologic pathology 0 40190623
2018 Tech news: stem cells for modeling and curing disease. BioTechniques 0 30477326
2008 A high-tech infusion for science. Disease models & mechanisms 0 19048066
2007 VA Tech disaster response shows value of drills, planning. Healthcare benchmarks and quality improvement 0 17520874
2000 A pharmacokinetic study of JOMO-tech in rats. European journal of drug metabolism and pharmacokinetics 0 11112087
1993 High-tech breakthrough DNA scanner for reading sequence and detecting gene mutation. A powerful 1 lb, 20 micron resolution, 16-bit personal scanner (PS) that scans 17" x 14" X-ray film in 48 s, with laser, UV and white light sources. Applied biochemistry and biotechnology 0 8379664

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