Affinage

NUMA1

Nuclear mitotic apparatus protein 1 · UniProt Q14980

Length
2115 aa
Mass
238.3 kDa
Annotated
2026-06-10
100 papers in source corpus 51 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUMA1 (NuMA) is a large coiled-coil protein that serves as the principal organizer of microtubule minus-ends at mitotic spindle poles and as the cortical force-transducer for spindle positioning (PMID:8898198, PMID:19255246). Structurally it is a 236-kDa molecule with a ~207-nm central parallel coiled-coil rod flanked by globular N- and C-terminal domains (PMID:1541630, PMID:1541636, PMID:7781599), the C-terminal globular domains driving self-assembly into multiarm oligomers that build an insoluble, microtubule-independent matrix at spindle poles (PMID:10075938, PMID:10098933). NuMA acts together with cytoplasmic dynein and dynactin, streaming poleward toward microtubule minus-ends in a mitosis-specific manner; this transport and pole assembly require intact dynein/dynactin (PMID:8898198, PMID:10811826), and NuMA functions as a dynein-activating adaptor through two LIC-binding sites (PMID:32413290) while also targeting dynactin and dynein activity directly to minus-ends via a dedicated C-terminal minus-end-binding module distinct from its microtubule-binding domain (PMID:29185983). At the cell cortex NuMA is recruited through the Gαi/LGN/Ric-8A pathway—LGN acting as a conformational switch that simultaneously engages NuMA and Gαi, with Ric-8A GEF activity dissociating the complex—to assemble clustered dynein–dynactin–NuMA modules that generate cooperative spindle-pulling forces (PMID:15537540, PMID:16275912, PMID:29848445); cortical targeting of NuMA alone suffices to pull the spindle (PMID:29848445). A parallel LGN/Gαi-independent route uses direct binding of NuMA to PtdInsP/PtdInsP2 and a C-terminal membrane-binding domain for anaphase cortical enrichment (PMID:24996901, PMID:24371089). NuMA's localization is gated by mitotic phosphorylation: CDK1 phosphorylation at T2055/T2040 and Plk1 phosphorylation restrain cortical binding, Aurora-A phosphorylation controls cytoplasm-to-pole exchange, and CDK1 inactivation/cyclin B degradation at anaphase releases NuMA from poles and promotes cortical accumulation (PMID:7769006, PMID:23921553, PMID:26832443, PMID:24371089, PMID:14710193, PMID:30456393). In vivo, NuMA is essential for spindle-pole focusing and kinetochore-fiber maintenance and for spindle orientation in epithelial tissues, where its microtubule-binding function controls epidermal differentiation (PMID:19255246, PMID:26765568). Beyond mitosis, NuMA contributes to interphase nuclear functions including PARsylation-dependent recruitment to DNA breaks and SNF2h-mediated homologous recombination (PMID:24753406), p53-selective p21 induction (PMID:23589328), rDNA transcription in the nucleolus (PMID:28981686), and 53BP1 sequestration (PMID:30812030), and in neurons it organizes axonal growth-cone microtubules and axon-initial-segment assembly (PMID:34793694, PMID:31727776). The NUMA1-RARA fusion underlies a variant of acute promyelocytic leukemia, establishing RARα dysregulation as the essential lesion (PMID:9288109).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1992 Medium

    Establishing NuMA's primary structure as a tripartite coiled-coil protein and its distinctive mitotic redistribution defined it as a candidate nuclear/spindle scaffold rather than a typical lamina component.

    Evidence cDNA cloning, sequence analysis, and dual immunofluorescence with anti-lamin antibodies

    PMID:1541630 PMID:1541636

    Open questions at the time
    • Function of the globular domains not yet assigned
    • No mechanism for how segregation pathway differs from lamins
  2. 1995 High

    Direct structural visualization confirmed the central rod as the longest known parallel coiled-coil flanked by globular heads, providing the architectural basis for self-assembly and microtubule cross-linking.

    Evidence Electron microscopy of recombinant NuMA with cross-linking and circular dichroism

    PMID:7781599

    Open questions at the time
    • Atomic structure of globular domains not resolved
    • Oligomerization state in cells not addressed
  3. 1995 High

    Immunodepletion and recombinant rescue in mitotic extracts established NuMA as functionally required for microtubule aster organization, settling whether it was merely a marker or an active organizer.

    Evidence Cell-free mitotic extract immunodepletion with recombinant add-back and in vitro aster assay

    PMID:7593190

    Open questions at the time
    • Molecular partners mediating aster assembly not identified
    • Phosphorylation requirement not yet mapped
  4. 1996 High

    Identifying NuMA in a complex with dynein/dynactin and showing its depletion abolishes spindle poles connected NuMA to the minus-end transport machinery.

    Evidence Xenopus egg extract immunodepletion, Co-IP, and aster assembly assays

    PMID:8898198

    Open questions at the time
    • Direct vs. indirect dynein binding not distinguished
    • Site of NuMA-dynein contact unknown
  5. 2000 High

    Live imaging of poleward NuMA streaming dependent on functional dynein/dynactin established that minus-end transport delivers NuMA to spindle poles.

    Evidence GFP live imaging, gel filtration, dominant-negative dynamitin, and dynein antibody inhibition

    PMID:10811826

    Open questions at the time
    • Whether NuMA activates dynein motility not yet tested
    • Minus-end recognition mechanism unknown
  6. 2001 High

    Discovery that LGN binds the NuMA C-terminal tail and controls spindle-pole organization opened the cortical/spindle-orientation arm of NuMA function.

    Evidence Co-IP, RNAi, and Xenopus egg extract aster assays

    PMID:11781568

    Open questions at the time
    • How LGN couples NuMA to the cortex not yet defined
    • Regulation of the interaction unknown
  7. 2002 High

    Mapping the tankyrase-binding RXXPDG motif and showing LGN sterically blocks NuMA's microtubule-stabilizing activity revealed competing C-terminal interactions controlling NuMA output.

    Evidence Yeast two-hybrid, in vitro binding, microtubule stabilization assays in extracts and cells

    PMID:12080061 PMID:12445386

    Open questions at the time
    • Physiological balance of competing interactions not quantified
    • In vivo consequence of tankyrase binding unresolved at this stage
  8. 2004 High

    FRET and biochemical work defined LGN as a Gαi-regulated conformational switch linking NuMA to the cortex, and cyclin B degradation as the trigger releasing NuMA from poles in anaphase.

    Evidence FRET biosensor, live imaging, and Xenopus extract with non-degradable cyclin B

    PMID:14710193 PMID:15537540

    Open questions at the time
    • Phosphatases reversing NuMA phosphorylation not identified here
    • Quantitative force output not measured
  9. 2005 Medium

    Demonstration that Ric-8A GEF activity catalytically dissociates Gαi/LGN/NuMA complexes and that NuMA is a major tankyrase-1 PARsylation acceptor added enzymatic regulators of cortical and spindle-pole NuMA.

    Evidence In vitro GEF assay with purified proteins; co-IP and siRNA for tankyrase-1/NuMA

    PMID:16076287 PMID:16275912

    Open questions at the time
    • Functional consequence of NuMA PARsylation for force generation untested here
    • In vivo timing of Ric-8A action not resolved
  10. 2009 High

    Conditional knockout and pADPr-binding studies separated NuMA's roles in pole establishment versus maintenance and showed poly(ADP-ribose) provides non-covalent cross-linking at poles.

    Evidence Mouse conditional knockout in primary cells; in vitro pADPr binding and bead aster assays

    PMID:19255246 PMID:19759176

    Open questions at the time
    • Relative contribution of pADPr vs. NuMA self-assembly to matrix not quantified
    • Centrosome-NuMA cooperation mechanism incomplete
  11. 2011 High

    Crystal structures of LGN/NuMA and LGN/mInsc complexes showed mutually exclusive TPR-repeat binding, defining the molecular logic by which polarity and spindle-tethering complexes are switched during asymmetric division.

    Evidence X-ray crystallography and in vitro competition binding

    PMID:21816348

    Open questions at the time
    • Temporal handoff between mInsc and NuMA in cells not directly visualized
    • Affinity regulation by phosphorylation not addressed
  12. 2013 High

    Phosphosite-specific work established CDK1 (T2055) as a negative regulator of cortical NuMA counteracted by PPP2CA, and identified a Gαi/LGN-independent membrane-binding domain enabling anaphase cortical enrichment.

    Evidence Phosphomutant expression, CDK1 inhibition, RNAi rescue, FRAP, and spindle orientation assays

    PMID:23921553 PMID:24109598 PMID:24371089

    Open questions at the time
    • Identity of the membrane lipid ligand not yet defined at this stage
    • Crosstalk between LGN and membrane routes unquantified
  13. 2014 High

    Identifying direct NuMA binding to phosphoinositides and DNA-damage-site recruitment via PARsylation/SNF2h extended NuMA function to a defined cortical lipid anchor and to homologous recombination.

    Evidence In vitro lipid-binding and PIP depletion assays; co-IP, FRAP, and DSB repair assays with CYLD/Dishevelled deubiquitination link

    PMID:24469800 PMID:24753406 PMID:24996901

    Open questions at the time
    • Structural basis of lipid recognition unresolved
    • Mechanism of NuMA's interphase nuclear-to-damage redistribution incomplete
  14. 2016 High

    Aurora-A was shown to directly phosphorylate the NuMA C-terminus to control cytoplasm-pole exchange, and a non-overlapping microtubule-binding domain was found required for spindle orientation in vivo.

    Evidence In vitro kinase assay, FRAP, phosphomutants, conditional mouse models, and Astrin binding mapping

    PMID:26765568 PMID:26832443 PMID:27462074

    Open questions at the time
    • Integration of Aurora-A with CDK1/Plk1 phosphoregulation not unified
    • How MT-binding controls differentiation mechanistically unclear
  15. 2017 High

    Defining NuMA as the factor that delivers dynactin to minus-ends, plus structural work on the Importin-α NLS and nucleolar/rDNA roles, refined both its spindle adaptor mechanism and its interphase nuclear functions.

    Evidence Laser-ablation minus-end assays, Importin-α/NuMA crystallography, and nucleolar co-IP/RNA-IP/ChIP

    PMID:28079116 PMID:28939615 PMID:28981686 PMID:29185983

    Open questions at the time
    • How importin masking is relieved at NEBD not fully detailed
    • Direct rDNA transcriptional mechanism of NuMA undefined
  16. 2018 Medium

    Optogenetic reconstitution demonstrated cortical NuMA clusters dynein-dynactin into cooperative force modules sufficient for spindle pulling, and Plk1 was added as a phosphoregulator of cortical NuMA.

    Evidence Light-induced cortical targeting, domain-deletion, FRAP, and Plk1 kinase/inhibitor assays

    PMID:29848445 PMID:30456393

    Open questions at the time
    • Stoichiometry of DDN clusters in vivo unmeasured
    • How clustering distinguishes pole-focusing from positioning unresolved
  17. 2019 Medium

    Acentrosomal-aster work and 53BP1/AIS studies broadened NuMA's role to spindle-independent aster nucleation, 53BP1 sequestration controlling repair pathway choice, and neuronal axon-initial-segment assembly.

    Evidence Acentrosomal cell system with live imaging; co-IP, single-molecule tracking, NHEJ assays; neuronal proteomics and shRNA

    PMID:30812030 PMID:31727776 PMID:31782546

    Open questions at the time
    • Mechanism linking nuclear NuMA pools to repair choice incomplete
    • Neuronal NuMA partners beyond 4.1B/Lis1 not fully mapped
  18. 2020 High

    Structural identification of two LIC-binding sites confirmed NuMA as a bona fide dynein-activating adaptor required for spindle placement.

    Evidence Structural/biochemical interface determination and mitotic functional mutagenesis

    PMID:32413290

    Open questions at the time
    • How both LIC sites cooperate during transport not resolved
    • Adaptor activity in interphase contexts untested
  19. 2021 Medium

    Phase-separation and neuronal-disease studies revealed NuMA forms condensates that concentrate spindle factors and that its loss recapitulates Huntington's disease growth-cone defects, extending NuMA into biophysical assembly and neuropathology.

    Evidence In vitro phase separation, Aurora-A regulation; neuronal proteomics, shRNA, antagomiR and microtubule-stabilizer rescue

    PMID:34793694 PMID:34887424

    Open questions at the time
    • Whether phase separation underlies the in vivo pole matrix not established
    • Direct molecular link between miR-124/NuMA and HD pathology incomplete

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple phosphoregulatory inputs (CDK1, Aurora-A, Plk1), competing C-terminal interactions, lipid binding, and phase separation are integrated to switch NuMA between its nuclear, spindle-pole, and cortical activities in space and time remains unresolved.
  • No unified quantitative model of NuMA phosphoregulation
  • Structure of the full-length NuMA matrix/condensate unknown
  • Relationship between interphase nuclear roles and mitotic functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5 GO:0005198 structural molecule activity 3 GO:0060090 molecular adaptor activity 3 GO:0008289 lipid binding 2 GO:0098772 molecular function regulator activity 2 GO:0003723 RNA binding 1
Localization
GO:0005815 microtubule organizing center 4 GO:0005886 plasma membrane 3 GO:0005634 nucleus 2 GO:0005856 cytoskeleton 2 GO:0005730 nucleolus 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-73894 DNA Repair 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
DCTN1EPB41GNAI1LGNSNF2HTNKSTP53TP53BP1
Complex memberships
Dynein-Dynactin-NuMA (DDN) cortical clusterGαi/LGN/NuMA cortical complexspindle pole matrix

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 NuMA forms a complex with cytoplasmic dynein and dynactin; immunodepletion of NuMA from frog egg extracts prevents normal mitotic spindle assembly, producing chromatin-associated irregular microtubule arrays lacking spindle poles. A subdomain of the NuMA tail mediates microtubule bundling and aster formation. Immunodepletion from Xenopus egg extracts, Co-IP, microtubule aster assembly assay Cell High 8898198
2000 NuMA is transported toward microtubule minus-ends at spindle poles via cytoplasmic dynein and dynactin. GFP-NuMA streams poleward in association with dynactin Arp1 subunit; disruption of dynactin (dynamitin overexpression) or dynein (antibody) blocks NuMA translocation and spindle pole assembly. NuMA-dynein-dynactin complex is mitosis-specific and reversible. GFP live imaging, immunoprecipitation, gel filtration, dominant-negative dynamitin overexpression, antibody inhibition The Journal of cell biology High 10811826
1995 NuMA is required for organization of microtubules into mitotic asters: immunodepletion from mammalian mitotic extracts abolishes aster formation, and addition of purified recombinant NuMA rescues aster assembly. NuMA is phosphorylated upon aster assembly and is required only in late stages of aster formation. Cell-free mitotic extract, immunodepletion, recombinant protein add-back, in vitro aster assembly assay The Journal of cell biology High 7593190
1992 NuMA encodes a 236-kDa protein with a large central coiled-coil domain (~1,485 aa) flanked by globular N- and C-terminal domains. NuMA dissociates from chromatin in early prophase before nuclear lamina breakdown, and reassociates with telophase chromosomes before lamins, defining a novel pathway for nuclear protein segregation at mitosis. cDNA cloning, sequence analysis, double immunofluorescence with anti-NuMA and anti-lamin antibodies The Journal of cell biology Medium 1541630 1541636
1993 Expression of NuMA lacking its globular head domain causes failure of cytokinesis and micronucleation. Expression of NuMA lacking its globular tail domain prevents targeting to interphase nuclei and mitotic spindle; cells transit mitosis normally but assemble micronuclei in daughter cells. Wild-type NuMA overexpression rescues nuclear assembly defects in RCC1-mutant tsBN2 cells. Dominant-negative mutant expression, cell cycle analysis, immunofluorescence, rescue experiment in temperature-sensitive cell line The Journal of cell biology High 8432734
1995 Mutation of threonine 2040 (a predicted p34cdc2 phosphorylation site) in NuMA abolishes its ability to associate with the mitotic spindle; instead, the mutant concentrates at the plasma membrane. Cells expressing these mutants have disorganized spindles and fail cytokinesis. NuMA is phosphorylated in a mitosis-specific manner in vivo. Site-directed mutagenesis, transient expression in tissue culture cells, immunofluorescence, in vivo phosphorylation labeling Journal of cell science High 7769006
2004 LGN (mammalian Pins) acts as a conformational switch linking NuMA to Gαi at the cell cortex. In its closed state, LGN N- and C-termini interact; NuMA or Gαi disrupts this, allowing simultaneous binding to both and cortical localization. NuMA binding to LGN is required for metaphase spindle oscillations. FRET biosensor, overexpression, dominant-negative analysis, live cell imaging Cell High 15537540
2001 LGN binds directly to the C-terminal tail of NuMA. LGN associates with spindle poles during mitosis; ectopic expression or siRNA knockdown of LGN disrupts spindle-pole organization. Anti-LGN antibodies and LGN-binding domain of NuMA trigger microtubule aster formation in Xenopus egg extracts. Co-immunoprecipitation, RNAi, Xenopus egg extract aster assay, immunofluorescence Nature cell biology High 11781568
2002 LGN blocks NuMA's ability to stabilize microtubules. NuMA C-terminus directly binds microtubules and stabilizes them; the LGN-binding domain on NuMA overlaps by 10 residues with the MT-binding domain. LGN exerts its inhibitory effect on spindle organization through steric exclusion of NuMA-MT interaction. In vitro microtubule stabilization assay, in vitro binding assay, Xenopus egg extracts, mammalian cell expression Current biology High 12445386
2005 NuMA is a major acceptor of poly(ADP-ribosyl)ation (PARsylation) by tankyrase 1 in mitosis. Association between tankyrase 1 and NuMA dramatically increases at mitotic onset, coincident with NuMA PARsylation. Tankyrase 1 siRNA knockdown eliminates NuMA PARsylation. NuMA siRNA knockdown causes complete loss of tankyrase 1 from spindle poles (NuMA is required for tankyrase 1 spindle pole localization). Immunoprecipitation, immunofluorescence, siRNA knockdown The Biochemical journal Medium 16076287
2002 NuMA contains an RXXPDG motif that mediates its binding to the ankyrin repeat domain of tankyrase 1 and tankyrase 2. NuMA was identified as a tankyrase partner by yeast two-hybrid and verified by direct binding assay. Yeast two-hybrid screen, in vitro binding assay The Journal of biological chemistry Medium 12080061
2009 Poly(ADP-ribose) (pADPr) from tankyrase 1 (PARP-5a) contributes to spindle pole assembly. The rod domain of NuMA binds directly to pADPr chains in vitro. pADPr-coated beads trigger aster assembly in mitotic lysates, suggesting pADPr provides dynamic cross-linking at spindle poles by binding NuMA non-covalently. Immunoelectron microscopy, concentrated mitotic lysate system, in vitro pADPr binding assay, pADPr-coated magnetic bead aster assembly Molecular biology of the cell Medium 19759176
1999 NuMA self-assembles in vitro into multiarm oligomers (up to 10–12 arms) via interaction of its C-terminal globular domains; each arm corresponds to a NuMA dimer. Computer modeling suggests 12-arm oligomers are structural units of the NuMA nuclear scaffold observed by EM in transfected cells. In vitro assembly of recombinant NuMA, electron microscopy, domain deletion analysis, computer modeling The EMBO journal Medium 10075938
1999 A 135-kDa nonerythroid isoform of protein 4.1R interacts directly with NuMA. The interaction is mediated by exons 20–21 of 4.1R and residues 1788–1810 of NuMA. 4.1R colocalizes with NuMA in interphase nuclei and at spindle poles; overexpression of 135-kDa 4.1R alters NuMA distribution in interphase nuclei. Yeast two-hybrid, in vitro binding assay, co-immunoprecipitation, co-immunolocalization The Journal of cell biology Medium 10189366
2009 NuMA is an essential mitotic component with distinct contributions to spindle pole establishment and maintenance. When NuMA mitotic function is disrupted using a conditional knockout in primary mouse cells, centrosomes provide initial focusing but centrosome attachment under tension is defective and kinetochore fiber maintenance at poles fails. Without both centrosomes and NuMA, spindle microtubule focusing completely fails. Conditional knockout allele in mice and primary cultured cells, immunofluorescence, live cell imaging The Journal of cell biology High 19255246
2013 CDK1-mediated phosphorylation of NuMA at T2055 negatively regulates NuMA cortical localization during metaphase; PPP2CA phosphatase activity counteracts this. CDK1 inactivation in anaphase leads to rise in dephosphorylated NuMA at cortex, increasing cortical dynein and promoting spindle elongation. Phosphomutant expression, CDK1 inhibitor experiments, siRNA, immunofluorescence, quantitative imaging The EMBO journal High 23921553
2014 NuMA directly associates with PtdInsP (PIP) and PtdInsP2 (PIP2) phosphoinositides in vitro, providing a LGN/Gαi-independent mechanism for cortical localization. Chemical or enzymatic depletion of PIP/PIP2 prevents NuMA cortical localization during mitosis; increasing PIP2 augments cortical NuMA. During anaphase, LGN/Gαi are dispensable for NuMA-dependent cortical dynein enrichment. In vitro lipid-binding assay, chemical/enzymatic PIP depletion, immunofluorescence, genetic knockdown The EMBO journal High 24996901
2016 Aurora-A directly phosphorylates NuMA C-terminus on three serine residues including Ser1969, controlling dynamic exchange of NuMA between cytoplasm and spindle poles. Partial Aurora-A inhibition causes NuMA/dynein accumulation at spindle poles without reaching cortex while LGN cortical distribution is unperturbed. A new microtubule-binding domain of NuMA was identified that does not overlap with the LGN-binding motif, allowing simultaneous binding to LGN and microtubules. In vitro kinase assay, FRAP, phosphomutant expression, Aurora-A partial inhibition, immunofluorescence Current biology High 26832443
2011 Crystal structures of LGN/NuMA and LGN/mInsc complexes show that NuMA and Inscuteable bind the same TPR repeats of LGN in a mutually exclusive manner, with mInsc binding with higher affinity. The Par3/mInsc/LGN and NuMA/LGN/Gαi complexes play sequential and overlapping roles in asymmetric cell division. X-ray crystallography, in vitro competition binding assay, cell biology Molecular cell High 21816348
2005 Ric-8A GEF activity catalytically dissociates Gαi-GDP/LGN/NuMA complexes in vitro, releasing activated Gαi-GTP and concomitantly liberating NuMA from LGN. Ric-8A efficiently uses GoLoco/Gαi-GDP complexes as substrates. In vitro GEF assay with purified proteins, biochemical dissociation experiment Proceedings of the National Academy of Sciences of the United States of America High 16275912
2010 Ric-8A and Gαi function to recruit LGN, NuMA, and dynein to the cell cortex for mitotic spindle orientation. Pertussis toxin (blocking Ric-8A GEF activity for Gαi), Ric-8A knockdown, or Gαi knockdown each impair cortical localization of LGN, NuMA, and dynein, and disturb integrin-dependent spindle orientation. Pertussis toxin treatment, siRNA knockdown, immunofluorescence, live cell imaging of GFP-tubulin Molecular and cellular biology Medium 20479129
2017 NuMA targets dynactin to microtubule minus-ends, localizing dynein activity there. NuMA is recruited to new minus-ends independently of dynein and faster than dynactin; both NuMA and dynactin show steady-state minus-end binding. NuMA localization to minus-ends requires a C-terminal region outside its canonical MT-binding domain. Both NuMA's minus-end-binding and dynein-dynactin-binding modules are required for focused bipolar spindle organization. Quantitative live imaging, laser ablation to generate new minus-ends, domain-deletion analysis, siRNA rescue experiments eLife High 29185983
2018 Cortical NuMA assembles specialized focal structures that cluster multiple dynein-dynactin force-generating modules (DDN clusters) to produce cooperative spindle-pulling forces. Induced cortical targeting of NuMA alone (not dynein) is sufficient for spindle pulling. This clustering activity is required for spindle positioning but not spindle-pole focusing. Light-induced cortical reconstitution system in human cells, live imaging, domain-deletion analysis, siRNA eLife High 29848445
2013 NuMA is required to recruit dynactin to the keratinocyte cell cortex. The 4.1-binding domain of NuMA stabilizes its cortical interaction (shown by FRAP); loss of 4.1/NuMA interaction causes spindle orientation defects. CDK1 inhibition or mutation of a single NuMA residue increases cortical NuMA localization in anaphase via an LGN- and 4.1-independent mechanism. siRNA, FRAP, phosphomutant expression, immunofluorescence, spindle orientation assay Molecular biology of the cell Medium 24109598
2016 NuMA's microtubule-binding domain, which targets microtubule tips, is required for spindle orientation in keratinocytes in addition to dynein/dynactin. Loss of NuMA-MT interactions in skin causes spindle orientation defects, epidermal differentiation defects, and neonatal lethality in mice. NuMA-MT interactions are also required in adult hair follicle matrix cells for proper differentiation. Conditional knockout/knock-in mice, MT-binding domain mutation, in vivo histology and immunofluorescence eLife High 26765568
2006 Rae1 interacts with NuMA in a mitosis-specific manner. A specific binding site for Rae1 on NuMA was mapped that would convert a NuMA dimer into a tetravalent MT crosslinker. Overexpression of Rae1 Rac binding domain of NuMA in HeLa cells leads to aberrant spindle formation; coupling Rae1 overexpression to NuMA overexpression or co-depleting both prevents aberrant spindles. Co-immunoprecipitation, domain mapping, overexpression and knockdown epistasis, immunofluorescence Proceedings of the National Academy of Sciences of the United States of America Medium 17172455
1999 NuMA forms an insoluble matrix at spindle poles distinct from pericentriolar material. Once incorporated into this matrix in vivo or in vitro, NuMA's insolubility is no longer dependent on microtubules. Immunodepletion of NuMA from mitotic extracts prevents formation of this insoluble matrix at aster cores. Immunogold electron microscopy, solubility fractionation, immunodepletion from mitotic extracts Cell motility and the cytoskeleton Medium 10098933
1997 The NuMA-RARA fusion protein from APL t(11;17) variant forms sheet-like nuclear aggregates with which normal NuMA partly co-localizes, while PML organization remains normal. This establishes that RARα dysregulation rather than PML disruption is essential for APL. Characterization of patient-derived fusion gene, immunofluorescence co-localization Nature genetics Medium 9288109
2013 Cell cycle-regulated membrane binding of NuMA via a Gαi/LGN-independent lipid/membrane-binding domain at the NuMA C-terminus underlies anaphase-specific cortical NuMA enrichment. CDK1 phosphorylation inhibits this membrane binding during prophase/metaphase; CDK1 inactivation at anaphase onset enables membrane binding. Membrane-binding-deficient NuMA specifically reduces anaphase cortical dynein, impairing chromosome separation. Domain mapping, phosphomutant expression, CDK1 inhibition, endogenous NuMA replacement by RNAi + rescue, immunofluorescence Molecular biology of the cell High 24371089
2004 Cyclin B degradation in anaphase leads to NuMA dephosphorylation and release from dynein/dynactin and from spindle poles in Xenopus egg extracts. Non-degradable cyclin B (Δ90) keeps NuMA phosphorylated, associated with dynein/dynactin, and locked at stable spindle poles that fail to disassemble. Xenopus egg extract, non-degradable cyclin B expression, immunoprecipitation, immunofluorescence EMBO reports Medium 14710193
1997 NuMA is phosphorylated during nuclear breakdown (G2/M transition) in a mitosis-specific manner. This phosphorylation occurs before spindle formation (present in nocodazole-treated cells) and does not require microtubule assembly. Dephosphorylation occurs in two distinct steps in early G1 and at end of G1. 32P-orthophosphate labeling of synchronized cells, phosphatase treatment, nocodazole arrest Journal of cell science Medium 9202389
2017 Crystal structure of Importin-α/NuMA-C-terminus complex reveals a novel NLS binding pattern. Importin-β, in the presence of Importin-α, inhibits the microtubule-binding function of NuMA by sterically masking a high-affinity MT-binding region C-terminal to the NLS. X-ray crystallography, in vitro microtubule-binding assay, domain mapping The Journal of cell biology High 28939615
2020 NuMA contains two LIC (light intermediate chain)-binding sites: a hook domain contacting LIC1 and LIC2 via a conserved hydrophobic patch, and a CC1-box homologous motif in the coiled-coil region. Both LIC-binding sites are essential for correct spindle placement and cell division, establishing NuMA as a dynein-activating adaptor. Structural studies (crystal/biochemical), mitotic cell functional assays, domain mutagenesis Structure High 32413290
2014 NuMA accumulates at DNA damage sites in a poly(ADP-ribosyl)ation-dependent manner and directly interacts with ISWI ATPase SNF2h. NuMA co-immunoprecipitates with SNF2h, regulates its nucleoplasmic diffusion, and controls its accumulation at DNA breaks. NuMA knockdown reduces chromatin decompaction after DNA cleavage, impairs recruitment of homologous recombination factors, and impairs DSB repair in chromosomal contexts. Co-immunoprecipitation, FRAP, siRNA knockdown, DNA damage assays, HR factor recruitment Nucleic acids research Medium 24753406
2013 NuMA binds to p53 and is required for selective induction of p21 (but not PUMA) following DNA damage. NuMA knockdown attenuates p21 induction and impairs cell cycle arrest. NuMA is required for recruitment of CDK8 (Mediator complex component) to the p21 promoter. Co-immunoprecipitation, siRNA knockdown, gene expression analysis, ChIP (CDK8 recruitment) Molecular and cellular biology Medium 23589328
2017 NuMA is present in the nucleolus and co-immunoprecipitates with RNA polymerase I, ribosomal proteins RPL26 and RPL24, and B-WICH chromatin remodeling complex components. NuMA binds 18S and 28S rRNAs and localizes to rDNA promoter regions. NuMA downregulation triggers nucleolar stress with decreased pre-rRNA synthesis and p27kip1 upregulation independent of p53. Co-immunoprecipitation, RNA immunoprecipitation, ChIP at rDNA promoters, siRNA knockdown, nascent RNA synthesis assay Nucleic acids research Medium 28981686
2019 NuMA interacts with 53BP1 and constrains 53BP1 diffusion throughout the nucleoplasm in the absence of DNA damage. This interaction is reduced after DNA damage. NuMA prevents 53BP1 accumulation at DNA breaks; NuMA knockdown enhances NHEJ and 53BP1-dependent activities. Co-immunoprecipitation, single-molecule tracking/FRAP, siRNA knockdown, NHEJ assay, immunoglobulin class switching Nucleic acids research Medium 30812030
2000 GAS41 binds in vitro to the C-terminal part of the NuMA rod region with Kd of 2×10⁻⁷ M. GAS41 shows a dotted nuclear staining in interphase. Interaction identified by yeast two-hybrid and confirmed by dot overlay and surface plasmon resonance. Yeast two-hybrid, dot overlay assay, surface plasmon resonance The Journal of biological chemistry Medium 10913114
2018 Plk1 directly interacts with and phosphorylates NuMA, reducing NuMA's cortical localization. Acute Plk1 inhibition during metaphase enriches cortical NuMA/dynein/LGN levels and alters NuMA dynamics at the cortex. This Plk1-NuMA phosphorylation is required for precise spindle orientation. In vitro kinase assay, Plk1 inhibitor (acute inactivation), phosphomutant expression, immunofluorescence, FRAP Life science alliance Medium 30456393
2021 NuMA undergoes liquid-liquid phase separation during mitotic entry; this is mediated by its C-terminus and facilitated by its dynein-dynactin binding motif. KifC1 promotes NuMA condensate concentration at spindle poles. Phase-separated NuMA droplets concentrate tubulins, bind microtubules, and enrich Kif2A, which depolymerizes spindle MTs for poleward flux. Aurora-A phosphorylation regulates NuMA phase separation. In vitro phase separation assay, live cell imaging, Aurora-A inhibition, siRNA knockdown, domain mutagenesis Nature communications Medium 34887424
2016 C-terminal tail of NuMA directly binds the C-terminus of Astrin, helping to recruit Astrin to microtubules. NuMA knockdown dramatically impairs Astrin spindle localization. Cytoplasmic dynein is required for spindle pole accumulation of Astrin. Reduced Astrin levels impair NuMA concentration at spindle poles (reciprocal dependency). In vitro direct binding assay, co-immunoprecipitation, RNAi knockdown, immunofluorescence The Journal of biological chemistry Medium 27462074
2014 CYLD deubiquitinase deubiquitinates the cortical polarity protein Dishevelled, which enhances Dishevelled's interaction with NuMA, stimulating cortical localization of NuMA and dynein/dynactin. This is required for generating pulling forces on astral microtubules for spindle orientation. Deubiquitination assay, co-immunoprecipitation, siRNA knockdown, immunofluorescence, spindle orientation assay Proceedings of the National Academy of Sciences of the United States of America Medium 24469800
2021 NUMA1 is downregulated in Huntington's disease neural progenitor growth cones by miR-124. NUMA1 regulates microtubule organization in axonal growth cones; suppressing NUMA1 recapitulates HD microtubule bundling defects and impairs axonal growth. Raising NUMA1 levels with antagomiR-124 or stabilizing microtubules rescues axonal growth defects. Proteomic analysis of growth cones, shRNA knockdown, antagomiR-124, epothilone B treatment, microtubule imaging in growth cones Neuron Medium 34793694
2020 NuMA1 is transiently localized at the axon initial segment (AIS) during development where it interacts with scaffolding protein 4.1B and dynein regulator Lis1. NuMA1 silencing disrupts AIS assembly (not maintenance). NuMA1 inhibits endocytosis of AIS protein neurofascin-186 by impeding Lis1's interaction with doublecortin. Differential proteomics, shRNA knockdown, overexpression, co-immunoprecipitation, endocytosis assay, immunofluorescence The Journal of cell biology Medium 31727776
2017 Katanin p80 regulates microtubule remodeling in combination with NuMA and cytoplasmic dynein. Depletion of p80 and/or NuMA induces abnormal mitotic phenotypes in mouse embryonic fibroblasts and aberrant neurogenesis and neuronal migration in mouse embryonic brain, placing p80 and NuMA in a common pathway for MT organization at spindle poles. siRNA knockdown, in utero electroporation in mouse brain, patient-derived iPSCs and brain organoids, immunofluorescence Scientific reports Medium 28079116
1995 Electron microscopy of recombinant full-length NuMA reveals a tripartite structure: a 207-nm long central rod domain that is a double-stranded parallel coiled-coil (the longest known at the time), flanked by globular N- and C-terminal domains. Chemical cross-linking and circular dichroism confirm the coiled-coil structure. Electron microscopy of purified recombinant NuMA, chemical cross-linking, circular dichroism spectroscopy The EMBO journal High 7781599
1997 NuMA is cleaved in apoptosis between residues 1701 and 1725 to produce a stable 180–200 kDa fragment. TPCK protease inhibitor retards both apoptotic morphology and NuMA cleavage, whereas ICE inhibitor II does not, suggesting a serine-type protease involvement. NuMA redistribution and cleavage are early nuclear events in apoptosis. Apoptosis induction, immunoblotting, protease inhibitor panel, immunofluorescence Experimental cell research Medium 9184071
2003 NuMA is preferentially cleaved by caspase-3 in vivo during Fas-mediated apoptosis (not cleaved in caspase-3-null MCF-7 cells). NuMA is cleaved coincidently with PARP-1 and lamin B; cleavage is inhibited by z-DEVD-FMK, z-VEID-FMK, and z-IETD-FMK caspase inhibitors. Caspase-3-null cell line, caspase inhibitors, immunoblotting, immunofluorescence Journal of cell science Medium 12508117
2004 NuMA undergoes continuous exchange between soluble and spindle-associated pools at spindle poles with ~3-minute half-time in living cells (FRAP). Protein kinase activity and LGN binding regulate NuMA dynamics: kinase inhibition increases, and LGN modulates the rate of NuMA exchange at spindle poles. FRAP in living cells, GFP-NuMA, in vitro aster displacement assay, kinase inhibitors Journal of cell science Medium 15561764
2019 NuMA assembles microtubule asters at nuclear envelope breakdown in acentrosomal human cells, and these asters are assembled via dynein and NuMA's clustering activity. NuMA-mediated asters incorporate Eg5 and facilitate spindle bipolarization. In cells with centrosomes, NuMA also promotes the initial step of spindle bipolarization in early mitosis. Acentrosomal human cell system, siRNA, immunofluorescence, live cell imaging The EMBO journal Medium 31782546

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell 488 8898198
2004 Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell 321 15537540
2000 Formation of spindle poles by dynein/dynactin-dependent transport of NuMA. The Journal of cell biology 280 10811826
2006 The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nature cell biology 267 16648843
2006 The Drosophila NuMA Homolog Mud regulates spindle orientation in asymmetric cell division. Developmental cell 251 16740476
2001 A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization. Nature cell biology 223 11781568
1995 NuMA is required for the organization of microtubules into aster-like mitotic arrays. The Journal of cell biology 220 7593190
1992 NuMA: an unusually long coiled-coil related protein in the mammalian nucleus. The Journal of cell biology 220 1541630
1997 Fusion of retinoic acid receptor alpha to NuMA, the nuclear mitotic apparatus protein, by a variant translocation in acute promyelocytic leukaemia. Nature genetics 211 9288109
1992 Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. The Journal of cell biology 207 1541636
1993 NuMA is required for the proper completion of mitosis. The Journal of cell biology 186 8432734
2010 Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle. Molecular and cellular biology 139 20479129
2010 NuMA after 30 years: the matrix revisited. Trends in cell biology 137 20137953
2005 NuMA is a major acceptor of poly(ADP-ribosyl)ation by tankyrase 1 in mitosis. The Biochemical journal 132 16076287
2002 Identification of a tankyrase-binding motif shared by IRAP, TAB182, and human TRF1 but not mouse TRF1. NuMA contains this RXXPDG motif and is a novel tankyrase partner. The Journal of biological chemistry 131 12080061
2002 LGN blocks the ability of NuMA to bind and stabilize microtubules. A mechanism for mitotic spindle assembly regulation. Current biology : CB 126 12445386
2011 A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells. The Journal of cell biology 123 21444683
2009 Requirements for NuMA in maintenance and establishment of mammalian spindle poles. The Journal of cell biology 120 19255246
2018 Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. eLife 111 29848445
2010 The Fz-Dsh planar cell polarity pathway induces oriented cell division via Mud/NuMA in Drosophila and zebrafish. Developmental cell 111 21074723
1999 A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein. The Journal of cell biology 111 10189366
2009 NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha. Nature cell biology 109 19219036
2011 LGN/mInsc and LGN/NuMA complex structures suggest distinct functions in asymmetric cell division for the Par3/mInsc/LGN and Gαi/LGN/NuMA pathways. Molecular cell 108 21816348
1995 Mutation of the predicted p34cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis. Journal of cell science 102 7769006
1999 Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice. The EMBO journal 100 10075938
2006 Rae1 interaction with NuMA is required for bipolar spindle formation. Proceedings of the National Academy of Sciences of the United States of America 96 17172455
2014 CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation. Proceedings of the National Academy of Sciences of the United States of America 95 24469800
1994 Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells. Cell motility and the cytoskeleton 95 7820866
2013 NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function. The EMBO journal 90 23921553
2017 NuMA recruits dynein activity to microtubule minus-ends at mitosis. eLife 88 29185983
1994 NuMA, a nuclear protein involved in mitosis and nuclear reformation. Current opinion in cell biology 85 7917323
2016 NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation. Current biology : CB 79 26832443
2014 SLK-dependent activation of ERMs controls LGN-NuMA localization and spindle orientation. The Journal of cell biology 78 24958772
2009 Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly. Molecular biology of the cell 78 19759176
1998 The role of NuMA in the interphase nucleus. Journal of cell science 78 9394013
2016 NuMA-microtubule interactions are critical for spindle orientation and the morphogenesis of diverse epidermal structures. eLife 77 26765568
2005 Resistance to inhibitors of cholinesterase 8A catalyzes release of Galphai-GTP and nuclear mitotic apparatus protein (NuMA) from NuMA/LGN/Galphai-GDP complexes. Proceedings of the National Academy of Sciences of the United States of America 77 16275912
2013 NuMA localization, stability, and function in spindle orientation involve 4.1 and Cdk1 interactions. Molecular biology of the cell 75 24109598
1986 Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein. Experimental cell research 75 3527729
1999 NuMA is a component of an insoluble matrix at mitotic spindle poles. Cell motility and the cytoskeleton 71 10098933
2011 Inscuteable and NuMA proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions. Proceedings of the National Academy of Sciences of the United States of America 67 22171003
2014 NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. The EMBO journal 65 24996901
1996 Dynamic changes of NuMA during the cell cycle and possible appearance of a truncated form of NuMA during apoptosis. Journal of cell science 65 8838651
2002 Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold. Experimental cell research 64 12243746
1998 Induction of a regular nuclear lattice by overexpression of NuMA. Experimental cell research 63 9743603
1996 NuMA assembles into an extensive filamentous structure when expressed in the cell cytoplasm. Journal of cell science 63 8907707
1993 Microinjection of a monoclonal antibody against SPN antigen, now identified by peptide sequences as the NuMA protein, induces micronuclei in PtK2 cells. Journal of cell science 63 8449992
2021 NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation. Nature communications 60 34887424
2006 Role of NuMA in vertebrate cells: review of an intriguing multifunctional protein. Frontiers in bioscience : a journal and virtual library 60 16146802
1995 NuMA: a protein involved in nuclear structure, spindle assembly, and nuclear re-formation. Trends in cell biology 60 14731413
2006 NuMA influences higher order chromatin organization in human mammary epithelium. Molecular biology of the cell 59 17108325
1997 Phosphorylation regulates the assembly of NuMA in a mammalian mitotic extract. Journal of cell science 59 9202389
2000 NuMA: a nuclear protein involved in mitotic centrosome function. Microscopy research and technique 58 10842374
1995 Epitope mapping and direct visualization of the parallel, in-register arrangement of the double-stranded coiled-coil in the NuMA protein. The EMBO journal 56 7781599
1994 Nuclear mitotic apparatus protein (NuMA): spindle association, nuclear targeting and differential subcellular localization of various NuMA isoforms. Journal of cell science 55 7962183
1984 NuMA protein is a human autoantigen. Arthritis and rheumatism 55 6378210
2011 aPKC phosphorylates NuMA-related LIN-5 to position the mitotic spindle during asymmetric division. Nature cell biology 54 21857670
1993 Nuclear proteins of the bovine esophageal epithelium. II. The NuMA gene gives rise to multiple mRNAs and gene products reactive with monoclonal antibody W1. Journal of cell science 54 8505359
2021 Developmental defects in Huntington's disease show that axonal growth and microtubule reorganization require NUMA1. Neuron 53 34793694
2014 NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks. Nucleic acids research 53 24753406
2013 Cell cycle-regulated membrane binding of NuMA contributes to efficient anaphase chromosome separation. Molecular biology of the cell 52 24371089
2004 Multiple mechanisms regulate NuMA dynamics at spindle poles. Journal of cell science 52 15561764
2019 NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells. The EMBO journal 50 31782546
2021 The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning. Frontiers in cell and developmental biology 49 33869212
1997 Cleavage of the nuclear matrix protein NuMA during apoptosis. Experimental cell research 49 9184071
2004 Cell and molecular biology of spindle poles and NuMA. International review of cytology 47 15364196
2013 Evidence for dynein and astral microtubule-mediated cortical release and transport of Gαi/LGN/NuMA complex in mitotic cells. Molecular biology of the cell 45 23389635
2003 A functional relationship between NuMA and kid is involved in both spindle organization and chromosome alignment in vertebrate cells. Molecular biology of the cell 44 12972545
2011 Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis. Proceedings of the National Academy of Sciences of the United States of America 43 21987826
2005 Association of the NuMA region on chromosome 11q13 with breast cancer susceptibility. Proceedings of the National Academy of Sciences of the United States of America 41 15684076
2004 Cyclin B degradation leads to NuMA release from dynein/dynactin and from spindle poles. EMBO reports 41 14710193
2000 GAS41, a highly conserved protein in eukaryotic nuclei, binds to NuMA. The Journal of biological chemistry 38 10913114
2020 The Aurora-A/TPX2 Axis Directs Spindle Orientation in Adherent Human Cells by Regulating NuMA and Microtubule Stability. Current biology : CB 37 33275894
2012 Interconnected contribution of tissue morphogenesis and the nuclear protein NuMA to the DNA damage response. Journal of cell science 37 22331358
2009 NuMA is required for proper spindle assembly and chromosome alignment in prometaphase. BMC research notes 37 19400937
1993 Primary structure and microtubule-interacting domain of the SP-H antigen: a mitotic MAP located at the spindle pole and characterized as a homologous protein to NuMA. Journal of cell science 37 8408288
2020 NuMA1 promotes axon initial segment assembly through inhibition of endocytosis. The Journal of cell biology 36 31727776
2017 Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics. Scientific reports 35 28079116
2017 The nuclear mitotic apparatus protein NuMA controls rDNA transcription and mediates the nucleolar stress response in a p53-independent manner. Nucleic acids research 34 28981686
2013 NuMA is required for the selective induction of p53 target genes. Molecular and cellular biology 34 23589328
2000 Preferential expression of NuMA in the nuclei of proliferating cells. Experimental cell research 34 10739661
1995 Phosphorylation of NUMA occurs during nuclear breakdown and not mitotic spindle assembly. Journal of cell science 34 8586651
2017 Regulation of mitotic spindle assembly factor NuMA by Importin-β. The Journal of cell biology 33 28939615
2004 Myeloid leukemia with promyelocytic features in transgenic mice expressing hCG-NuMA-RARalpha. Oncogene 33 14737102
2016 SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by Negatively Regulating the Gαi-LGN-NuMA Ternary Complex. Developmental cell 32 26766442
2013 Par1b links lumen polarity with LGN-NuMA positioning for distinct epithelial cell division phenotypes. The Journal of cell biology 32 24165937
1996 NuMA: a bipartite nuclear location signal and other functional properties of the tail domain. Experimental cell research 32 8635513
2018 Plk1 regulates spindle orientation by phosphorylating NuMA in human cells. Life science alliance 31 30456393
2019 The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic acids research 30 30812030
1999 The plant nucleoskeleton: ultrastructural organization and identification of NuMA homologues in the nuclear matrix and mitotic spindle of plant cells. Experimental cell research 30 9925768
2003 NuMA and nuclear lamins behave differently in Fas-mediated apoptosis. Journal of cell science 28 12508117
2014 Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells. BMC developmental biology 27 25527079
2012 On the inscrutable role of Inscuteable: structural basis and functional implications for the competitive binding of NuMA and Inscuteable to LGN. Open biology 27 22977735
2020 Organizational Principles of the NuMA-Dynein Interaction Interface and Implications for Mitotic Spindle Functions. Structure (London, England : 1993) 26 32413290
2016 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates Astrin at the Mitotic Spindle. The Journal of biological chemistry 26 27462074
2011 The nuclear mitotic apparatus (NuMA) protein: localization and dynamics in human oocytes, fertilization and early embryos. Molecular human reproduction 25 21297155
2017 Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Scientific reports 24 28469279
2002 Association of the nuclear matrix component NuMA with the Cajal body and nuclear speckle compartments during transitions in transcriptional activity in lens cell differentiation. European journal of cell biology 24 12437190
2003 Essential role for the dimerization domain of NuMA-RARalpha in its oncogenic activities and localization to NuMA sites within the nucleus. Oncogene 23 12584566
2012 NuMA overexpression in epithelial ovarian cancer. PloS one 22 22719996

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