| 2006 |
NOL7 localizes to the nucleolus and functions as a tumor suppressor in cervical cancer; reintroduction into cervical carcinoma cells suppressed in vivo tumor growth and induced an angiogenic switch characterized by decreased VEGF production and increased thrombospondin-1 (TSP-1) production. |
Transfection/xenograft assay, fluorescence in situ hybridization (LOH analysis), protein localization |
Oncogene |
Medium |
16205646
|
| 2010 |
NOL7 requires cytosolic machinery for active nuclear import; three functional nuclear localization signals (NLSs) were identified, with NLS2 and NLS3 critical for rate and efficiency of nuclear targeting; four basic clusters within NLS2 and NLS3 independently mediate nucleolar targeting; nucleolar occupancy involves rapid nucleoplasmic shuttling but low nucleolar mobility; depletion of RNA (total or rRNA) causes a nucleoplasmic shift of NOL7, indicating RNA-dependent nucleolar targeting. |
In vitro nuclear import assay, deletion constructs, cytoplasmic pyruvate kinase fusion proteins, site-directed mutagenesis, FRAP, RNA depletion experiments |
BMC cell biology |
High |
20875127
|
| 2010 |
The NOL7 promoter is a TATA-less promoter regulated by transcription factors SP1, c-Myc, and RXRalpha, which bind to its promoter region; c-Myc and RXRalpha positively regulate NOL7 expression. |
5' RACE (transcriptional start site mapping), promoter deletion analysis, chromatin immunoprecipitation (ChIP) |
Gene |
Medium |
20206243
|
| 2011 |
Three alternatively spliced NOL7 isoforms exist with distinct localizations: SP1 (29 kD, long form) localizes to the nucleolar granular component and dissociates from the nucleolus during cell division; SP2 is nucleoplasmic; SP3 is cytoplasmic. Knockdown of NOL7-SP1 abrogates nucleolar architecture (particularly internal regions) and reduces cell proliferation. Nucleolar targeting is mediated by a C-terminal nucleolar localization sequence (NoLS), but high nucleolar binding affinity requires additional protein regions beyond the NoLS. |
Alternative splice isoform cloning, fluorescence localization, siRNA knockdown, domain dissection |
Nucleus |
Medium |
21818416
|
| 2012 |
RB tumor suppressor positively regulates NOL7 at the transcriptional level by recruiting transcription factors and transcription machinery proteins to the NOL7 promoter; loss of RB represses NOL7 transcription by inhibiting assembly of these proteins at the promoter. |
Chromatin immunoprecipitation (ChIP), transcriptional reporter assays, RB loss-of-function analysis |
Neoplasia |
Medium |
23308053
|
| 2012 |
NOL7 is an mRNA-binding protein that must localize to the nucleoplasm to exert its antiangiogenic effects; it associates with RNA-processing machinery and directly binds the 3'UTR of TSP-1 mRNA, increasing TSP-1 mRNA stability (increased mRNA half-life) and protein levels; NOL7-mediated post-transcriptional stabilization also affects a broader subset of angiogenesis-related mRNAs. |
RNA immunoprecipitation, 3'UTR luciferase reporter assay, mRNA half-life assay, RNA-binding protein characterization, localization mutant analysis |
Oncogene |
High |
23085760
|
| 2023 |
NOL7 is the likely human ortholog of yeast Bud21 and functions as a U3 Associated Protein (UTP) subunit of the small subunit processome; it is required for early pre-rRNA accumulation and pre-18S rRNA processing in human cells; NOL7 depletion leads to decreased protein synthesis and induction of the nucleolar stress response. |
siRNA knockdown, pre-rRNA processing assays (Northern blot/primer extension), protein synthesis assays, nucleolar stress response assays |
RNA biology |
High |
37246770
|
| 2024 |
PQBP3/NOL7 directly binds PSME3 (proteasome activator complex subunit 3), decreasing PSME3 interaction with Lamin B1 and thereby preventing proteasomal Lamin B1 degradation; depletion of PQBP3/NOL7 causes nuclear membrane instability and release of genomic DNA to the cytosol, triggering senescence. Mutant ataxin-1 (ATXN1) sequesters PQBP3 into inclusion bodies, reducing nucleolar PQBP3 levels and causing a senescence phenotype in Purkinje cells in knock-in mice. |
Co-immunoprecipitation (direct binding assay), knockdown/depletion experiments, knock-in mouse model, nuclear membrane integrity assays, cytosolic DNA detection |
The EMBO journal |
High |
39103492
|
| 2024 |
PQBP3/NOL7 is an intrinsically disordered protein (IDP); bioinformatics and high-speed atomic force microscopy (HS-AFM) demonstrate that its N-terminal region is dynamically unstructured. |
Bioinformatics disorder prediction, high-speed atomic force microscopy (HS-AFM) |
Biochemical and biophysical research communications |
Medium |
39126896
|
| 2026 |
NOL7 binds the 3'UTR of GADD45A mRNA, stabilizing GADD45A mRNA and upregulating GADD45A expression; the NOL7/GADD45A axis suppresses STAT3 phosphorylation at Ser727, thereby inhibiting ovarian cancer cell proliferation and angiogenesis. |
RNA immunoprecipitation, 3'UTR reporter assay, mRNA stability assay, western blot for pSTAT3-Ser727, in vivo xenograft |
Cancer science |
Medium |
41812272
|