Affinage

MAPRE2

Microtubule-associated protein RP/EB family member 2 · UniProt Q15555

Round 2 corrected
Length
327 aa
Mass
37.0 kDa
Annotated
2026-04-28
130 papers in source corpus 9 papers cited in narrative 9 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAPRE2 (EB2) is a microtubule plus-end tracking protein that governs microtubule dynamics in both interphase and mitosis, with specialized roles in epithelial differentiation, cell migration, and intracellular trafficking. During mitosis, Aurora B and CDK1 phosphorylate MAPRE2 to reduce its microtubule affinity, ensuring proper kinetochore–microtubule dynamics and faithful chromosome segregation; non-phosphorylatable MAPRE2 stabilizes kinetochore microtubules and causes aneuploidy (PMID:27030108). In interphase, MAPRE2 promotes focal adhesion turnover and cell migration through a specific interaction with HAX1 (PMID:26527684) and facilitates anterograde trafficking of the TRPM4 channel to the plasma membrane via a SxIP-like motif (PMID:31112396); it also uniquely drives initial microtubule reorganization during apico-basal epithelial differentiation, where its depletion leads to aberrantly stable, bundled microtubules (PMID:23813963). Gain-of-function mutations in MAPRE2 that enhance microtubule dwell time cause Circumferential Skin Creases Kunze Type (CSC-KT) syndrome (PMID:26637975).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 2006 Low

    Detection of MAPRE2 as a phosphoprotein with EGF-responsive phosphosites established it as a signaling-responsive cytoskeletal regulator, though the functional relevance of individual sites was unknown.

    Evidence SILAC-based quantitative phosphoproteomics in EGF-stimulated HeLa cells

    PMID:17081983

    Open questions at the time
    • No functional validation of any specific MAPRE2 phosphorylation site
    • No kinase assignment
    • Single large-scale screen without targeted follow-up
  2. 2013 High

    Demonstration that EB2, but not EB1, is essential for initial microtubule reorganization during apico-basal epithelial differentiation revealed a non-redundant, tissue-specific function for MAPRE2 in controlling microtubule architecture.

    Evidence siRNA knockdown in differentiating epithelial cells, live-cell microtubule dynamics imaging, in situ tissue analysis, formin inhibitor rescue

    PMID:23813963

    Open questions at the time
    • Direct binding partners mediating the EB2-specific reorganization signal not identified
    • Whether EB2 acts through suppressing formin-dependent bundling or via an independent route remains unclear
  3. 2015 High

    Identification of MAPRE2 mutations as the cause of CSC-KT syndrome, combined with evidence that mutant proteins display enhanced microtubule binding, established that aberrant plus-end dwell time is pathogenic and linked MAPRE2 to a developmental tubulinopathy.

    Evidence Patient mutation identification, biochemical microtubule-binding assays of mutant proteins, zebrafish craniofacial developmental model

    PMID:26637975

    Open questions at the time
    • Precise downstream signaling pathways disrupted by enhanced EB2–microtubule binding in affected tissues not defined
    • Structural basis for altered dwell time of mutant proteins not resolved
  4. 2015 High

    Discovery of the EB2–HAX1 interaction and its requirement for focal adhesion turnover and cell migration provided the first defined molecular partnership explaining how EB2 regulates adhesion-coupled motility.

    Evidence Quantitative proteomics interactor screen, reciprocal co-immunoprecipitation, siRNA knockdown with focal adhesion dynamics and migration readouts in vitro and in vivo

    PMID:26527684

    Open questions at the time
    • Structural basis of the EB2–HAX1 interface not determined
    • Whether HAX1 mediates EB2's role in all motility contexts or only in epidermal cells is unknown
  5. 2016 High

    Mapping of Aurora B and CDK1 phosphosites on MAPRE2 and demonstration that phosphorylation reduces microtubule affinity during mitosis resolved how cells temporally control EB2 activity to enable proper chromosome segregation.

    Evidence In vitro kinase assays, phospho-site mass spectrometry, non-phosphorylatable mutant expression, live-cell imaging of mitotic progression, aneuploidy quantification

    PMID:27030108

    Open questions at the time
    • Phosphatases responsible for MAPRE2 dephosphorylation at mitotic exit not identified
    • Relative contributions of Aurora B versus CDK1 to in vivo MAPRE2 regulation not deconvolved
  6. 2019 Medium

    Demonstration that EB2 mediates anterograde trafficking of TRPM4 to the plasma membrane via a SxIP-like motif expanded EB2's functional repertoire beyond cytoskeletal regulation to cargo-specific vesicular transport.

    Evidence Co-immunoprecipitation, SxIP motif mutagenesis of TRPM4, EB2 siRNA knockdown with subcellular localization, focal adhesion and invasion assays

    PMID:31112396

    Open questions at the time
    • Whether EB2 delivers other SxIP-containing cargoes to the plasma membrane not tested
    • Single-lab finding without independent replication
  7. 2020 Medium

    Identification of WDHD1-mediated ubiquitination and degradation of MAPRE2 in the nucleus, with consequences for cisplatin sensitivity, revealed a post-translational mechanism controlling MAPRE2 protein levels in cancer cells.

    Evidence Co-immunoprecipitation, ubiquitination assay, MAPRE2 knockout cisplatin sensitivity readout, xenograft experiments in A549 lung adenocarcinoma cells

    PMID:32426268

    Open questions at the time
    • Specific ubiquitin chain linkage type on MAPRE2 not characterized
    • Nuclear function of MAPRE2 that would explain cisplatin sensitivity phenotype is undefined
    • Single-lab finding

Open questions

Synthesis pass · forward-looking unresolved questions
  • A unified structural and biophysical model explaining how MAPRE2 phosphorylation, disease mutations, and partner interactions (HAX1, TRPM4) converge on microtubule dwell-time control is still lacking.
  • No high-resolution structure of MAPRE2 bound to microtubule plus ends or partner proteins
  • In vivo tissue-specific functions beyond epithelium and craniofacial development largely unexplored
  • The nuclear pool of MAPRE2 and its functional significance remain poorly defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5
Localization
GO:0005856 cytoskeleton 5 GO:0005634 nucleus 1 GO:0005886 plasma membrane 1
Pathway
R-HSA-1266738 Developmental Biology 2 R-HSA-1500931 Cell-Cell communication 1 R-HSA-1640170 Cell Cycle 1 R-HSA-1852241 Organelle biogenesis and maintenance 1
Partners
AURKBCDK1HAX1TRPM4WDHD1

Evidence

Reading pass · 9 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2015 Mutations in MAPRE2 cause Circumferential Skin Creases Kunze Type (CSC-KT) syndrome. Disease-associated MAPRE2 mutations result in enhanced binding of MAPRE2 to microtubules, implying an increased dwell time at microtubule plus ends. In vivo zebrafish analysis showed MAPRE2 variants perturb branchial arch patterning, either through excessive activity (recessive paradigm) or haploinsufficiency (dominant de novo paradigm). Patient mutation identification, functional microtubule-binding assay of mutant MAPRE2 proteins, zebrafish craniofacial developmental model (in vivo loss-of-function and gain-of-function) American journal of human genetics High 26637975
2016 Aurora B and CDK1 phosphorylate MAPRE2 (EB2) at multiple sites within the amino terminus and a serine/threonine cluster in the linker domain connecting the calponin homology and end-binding homology domains. This phosphorylation is strictly associated with mitotic entry and progression and reduces MAPRE2 binding affinity for microtubules. Expression of non-phosphorylatable MAPRE2 induces stable kinetochore microtubule dynamics, delays bipolar metaphase plate formation in a microtubule binding-dependent manner, and leads to aneuploidy in unperturbed mitosis. In vitro kinase assays with purified Aurora B and CDK1, phospho-site mapping by mass spectrometry, non-phosphorylatable mutant expression, live-cell imaging of mitotic progression, aneuploidy quantification Nature communications High 27030108
2013 MAPRE2 (EB2), but not EB1, is essential for initial microtubule reorganization during apico-basal epithelial differentiation. EB2 siRNA knockdown during early differentiation prevented microtubule reorganization, while knockdown at later stages promoted microtubule stability and bundle formation. Depletion of EB2 in undifferentiated epithelial cells induced formation of straight, less dynamic microtubules with EB1 and ACF7 lattice association and co-alignment with actin filaments, a phenotype rescued by formin inhibition. In situ tissue analysis revealed inverse correlation between EB2 expression level and presence of apico-basal microtubule bundles. siRNA knockdown, live-cell microtubule dynamics imaging, immunofluorescence, in situ tissue analysis (inner ear and intestinal crypt epithelium), formin inhibitor rescue Journal of cell science High 23813963
2015 MAPRE2 (EB2) interacts specifically with HAX1 (HCLS1-associated protein X-1) as identified by quantitative proteomics. Knockdown of either HAX1 or EB2 in skin epidermal cells stabilizes focal adhesions and impairs epidermal cell migration in vitro and in vivo. The EB2-Hax1 interaction is required for focal adhesion turnover and cell motility. Quantitative proteomics (EB2-specific interactor identification), co-immunoprecipitation validation, siRNA knockdown of EB2 and HAX1, focal adhesion dynamics assay, in vitro and in vivo migration assays The Journal of biological chemistry High 26527684
2009 MAPRE2 expression is significantly increased in highly nerve-invasive pancreatic cancer cells compared to less invasive cells. Changes in MAPRE2 expression result in altered actin distribution in pancreatic cancer cells. MAPRE2 is predominantly expressed in normal pancreatic acinar cells but absent in ductal cells, while pancreatic cancer shows strong cytoplasmic and occasional nuclear MAPRE2 expression. Comparative gene expression analysis between high and low nerve-invasive cell lines, MAPRE2 overexpression/knockdown with actin distribution readout by immunofluorescence, immunohistochemistry of patient tissues International journal of oncology Medium 19787265
2020 The ubiquitin ligase WDHD1 interacts with MAPRE2 in the nucleus and promotes MAPRE2 ubiquitination, leading to its degradation. WDHD1 knockout upregulates MAPRE2, while MAPRE2 knockout in A549 lung adenocarcinoma cells increases cell viability and decreases apoptosis upon cisplatin exposure. Thus WDHD1 induces cisplatin resistance partly by promoting MAPRE2 ubiquitination and reducing MAPRE2 protein levels. Co-immunoprecipitation (WDHD1-MAPRE2 interaction in nucleus), protein stability assay, ubiquitination assay, MAPRE2 knockout (cisplatin sensitivity readout), animal xenograft experiments Frontiers in oncology Medium 32426268
2019 MAPRE2 (EB2) is required for anterograde trafficking of the TRPM4 channel to the plasma membrane. EB2 interacts with TRPM4 via a SxIP-like motif in TRPM4; mutations of this motif abolish the TRPM4-EB2 interaction, reduce plasma membrane TRPM4 expression, and cause ER retention. EB2 depletion impairs TRPM4-dependent focal adhesion disassembly and cell invasion. Bioinformatics motif identification, co-immunoprecipitation, site-directed mutagenesis of TRPM4 EB-binding motif, immunofluorescence subcellular localization, EB2 siRNA knockdown, focal adhesion and invasion assays FASEB journal Medium 31112396
2010 A human cytomegalovirus microRNA (miR-US25-1) targets the 5'UTR of MAPRE2 mRNA to mediate repression of MAPRE2 expression, as part of a coordinated suppression of multiple cell cycle-related genes. RISC immunoprecipitation, reporter assays with MAPRE2 5'UTR, miRNA deletion from HCMV genome PLoS pathogens Medium 20585629
2006 MAPRE2 was identified as a phosphoprotein in a global mass spectrometric phosphoproteomics screen of HeLa cells stimulated with EGF, detecting specific phosphorylation sites on MAPRE2 and their temporal dynamics. Global phosphoproteomic mass spectrometry (SILAC-based quantitative MS after EGF stimulation) Cell Low 17081983

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2000 DNA cloning using in vitro site-specific recombination. Genome research 815 11076863
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
1995 APC binds to the novel protein EB1. Cancer research 392 7606712
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2005 CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. The Journal of cell biology 336 15631994
2000 Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO reports 281 11256614
2022 Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Cell 256 35063084
2000 EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability. The Journal of cell biology 227 10811817
2016 An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. Nature communications 211 27173435
2007 Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1. Molecular cell 210 17889670
2011 Toward an understanding of the protein interaction network of the human liver. Molecular systems biology 207 21988832
2018 An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications 201 29568061
2020 Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions. Nature cell biology 194 32203420
1998 EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle. Oncogene 189 10030671
2002 EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. Molecular biology of the cell 184 12388761
2010 A viral microRNA down-regulates multiple cell cycle genes through mRNA 5'UTRs. PLoS pathogens 174 20585629
2014 EB1 accelerates two conformational transitions important for microtubule maturation and dynamics. Current biology : CB 165 24508171
2002 Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Molecular biology of the cell 161 12388762
1998 The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. Proceedings of the National Academy of Sciences of the United States of America 157 9724749
2005 Essential roles of IGFBP-3 and IGFBP-rP1 in breast cancer. European journal of cancer (Oxford, England : 1990) 120 15979304
2005 TIP maker and TIP marker; EB1 as a master controller of microtubule plus ends. The Journal of cell biology 115 16247021
2000 Disease expression of RP1 mutations causing autosomal dominant retinitis pigmentosa. Investigative ophthalmology & visual science 105 10845615
2017 Regulation of end-binding protein EB1 in the control of microtubule dynamics. Cellular and molecular life sciences : CMLS 95 28204846
2003 Shortstop recruits EB1/APC1 and promotes microtubule assembly at the muscle-tendon junction. Current biology : CB 94 12842007
2012 Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interacting proteins). The Journal of neuroscience : the official journal of the Society for Neuroscience 89 22764224
2011 Kif18B interacts with EB1 and controls astral microtubule length during mitosis. Molecular biology of the cell 85 21737685
2003 Characterization of functional domains of human EB1 family proteins. The Journal of biological chemistry 84 14514668
2008 EB1 promotes Aurora-B kinase activity through blocking its inactivation by protein phosphatase 2A. Proceedings of the National Academy of Sciences of the United States of America 82 18477699
2005 Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. The Journal of biological chemistry 81 16129689
2005 Melanophilin and myosin Va track the microtubule plus end on EB1. The Journal of cell biology 77 16247022
1999 Mutations in the RP1 gene causing autosomal dominant retinitis pigmentosa. Human molecular genetics 77 10484783
2002 A novel nuclear export signal and a REF interaction domain both promote mRNA export by the Epstein-Barr virus EB2 protein. The Journal of biological chemistry 74 12403791
2014 Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. The Journal of cell biology 73 25225338
2009 Essential and synergistic roles of RP1 and RP1L1 in rod photoreceptor axoneme and retinitis pigmentosa. The Journal of neuroscience : the official journal of the Society for Neuroscience 73 19657028
2002 Dissecting interactions between EB1, microtubules and APC in cortical clusters at the plasma membrane. Journal of cell science 72 11950877
2002 Epstein-Barr virus mRNA export factor EB2 is essential for production of infectious virus. Journal of virology 72 12208942
2022 Phase separation of EB1 guides microtubule plus-end dynamics. Nature cell biology 67 36536176
2016 EB1 interacts with outwardly curved and straight regions of the microtubule lattice. Nature cell biology 66 27617931
2015 Mutations in Either TUBB or MAPRE2 Cause Circumferential Skin Creases Kunze Type. American journal of human genetics 65 26637975
2001 Clinical features and mutations in patients with dominant retinitis pigmentosa-1 (RP1). Investigative ophthalmology & visual science 64 11527933
2017 EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. The Journal of cell biology 63 28814570
2019 KLF5 regulated lncRNA RP1 promotes the growth and metastasis of breast cancer via repressing p27kip1 translation. Cell death & disease 62 31073122
2021 Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis. Nature chemical biology 61 34608293
2000 Epstein-Barr virus EB2 protein exports unspliced RNA via a Crm-1-independent pathway. Journal of virology 61 10846090
2018 EB1 binding restricts STIM1 translocation to ER-PM junctions and regulates store-operated Ca2+ entry. The Journal of cell biology 60 29563214
2018 Biological functions and clinical significance of the newly identified long non‑coding RNA RP1‑85F18.6 in colorectal cancer. Oncology reports 60 30226619
2018 Local control of intracellular microtubule dynamics by EB1 photodissociation. Nature cell biology 59 29379139
2007 MCAK associates with EB1. Oncogene 59 17968321
2003 A region of the Epstein-Barr virus (EBV) mRNA export factor EB2 containing an arginine-rich motif mediates direct binding to RNA. The Journal of biological chemistry 56 12857728
2014 Binding to Cep164, but not EB1, is essential for centriolar localization of TTBK2 and its function in ciliogenesis. Genes to cells : devoted to molecular & cellular mechanisms 55 25297623
2003 CLIP-170 interacts with dynactin complex and the APC-binding protein EB1 by different mechanisms. Cell motility and the cytoskeleton 54 12789661
2010 Effect of GFP tags on the localization of EB1 and EB1 fragments in vivo. Cytoskeleton (Hoboken, N.J.) 53 19701929
2007 BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment. The Journal of cell biology 52 17709426
2012 GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migration. PloS one 49 23236459
2015 TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation. The Journal of cell biology 47 26323690
2001 Regulation of microtubule assembly by human EB1 family proteins. Oncogene 47 11423968
2014 Superresolution imaging reveals structural features of EB1 in microtubule plus-end tracking. Molecular biology of the cell 43 25355949
2013 The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation. Journal of cell science 43 23813963
1975 RP1 properties and fertility inhibition among P, N, W, and X incompatibility group plasmids. Journal of bacteriology 43 1095558
2014 The microtubule-associated protein EB1 links AIM2 inflammasomes with autophagy-dependent secretion. The Journal of biological chemistry 42 25164813
2012 IGFBP-rP1 induces p21 expression through a p53-independent pathway, leading to cellular senescence of MCF-7 breast cancer cells. Journal of cancer research and clinical oncology 42 22392074
2000 RP1 protein truncating mutations predominate at the RP1 adRP locus. Investigative ophthalmology & visual science 40 11095597
2013 EB1, p150Glued, and Clasp1 control endothelial tubulogenesis through microtubule assembly, acetylation, and apical polarization. Blood 38 23444400
2009 Epstein-Barr virus protein EB2 contains an N-terminal transferable nuclear export signal that promotes nucleocytoplasmic export by directly binding TAP/NXF1. Journal of virology 37 19793817
2009 EB1 acts as an oncogene via activating beta-catenin/TCF pathway to promote cellular growth and inhibit apoptosis. Molecular carcinogenesis 36 18680107
2005 Interaction between EB1 and p150glued is required for anaphase astral microtubule elongation and stimulation of cytokinesis. Current biology : CB 36 16360686
2011 RP1 and autosomal dominant rod-cone dystrophy: novel mutations, a review of published variants, and genotype-phenotype correlation. Human mutation 35 22052604
2015 Compartmentalized Toxoplasma EB1 bundles spindle microtubules to secure accurate chromosome segregation. Molecular biology of the cell 34 26466679
2014 Kif4 interacts with EB1 and stabilizes microtubules downstream of Rho-mDia in migrating fibroblasts. PloS one 34 24658398
2014 ROS-mediated EB1 phosphorylation through Akt/GSK3β pathway: implication in cancer cell response to microtubule-targeting agents. Oncotarget 34 24930764
2009 Migration and actin protrusion in melanoma cells are regulated by EB1 protein. Cancer letters 34 19427113
2016 ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules. Cell discovery 33 27721984
2014 IQGAP1 regulates endothelial barrier function via EB1-cortactin cross talk. Molecular and cellular biology 33 25022754
2005 Epstein-Barr virus mRNA export factor EB2 is essential for intranuclear capsid assembly and production of gp350. Journal of virology 33 16254345
2009 XMAP215-EB1 interaction is required for proper spindle assembly and chromosome segregation in Xenopus egg extract. Molecular biology of the cell 32 19369422
2014 CYLD coordinates with EB1 to regulate microtubule dynamics and cell migration. Cell cycle (Georgetown, Tex.) 31 24552808
2012 Epstein-Barr virus protein EB2 stimulates cytoplasmic mRNA accumulation by counteracting the deleterious effects of SRp20 on viral mRNAs. Nucleic acids research 31 22505578
2008 Retinitis pigmentosa: mutation analysis of RHO, PRPF31, RP1, and IMPDH1 genes in patients from India. Molecular vision 31 18552984
2007 Protein kinase CK2 phosphorylation of EB2 regulates its function in the production of Epstein-Barr virus infectious viral particles. Journal of virology 31 17699575
2018 Protein interactions of FAM134B with EB1 and APC/beta-catenin in vitro in colon carcinoma. Molecular carcinogenesis 30 29964340
2016 Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nature communications 30 27030108
2003 De novo mutation in the RP1 gene (Arg677ter) associated with retinitis pigmentosa. Investigative ophthalmology & visual science 30 12882812
2014 Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line. Journal of ginseng research 29 25379004
2009 The microtubule-associated protein MAPRE2 is involved in perineural invasion of pancreatic cancer cells. International journal of oncology 29 19787265
2019 Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent manner. Molecular biology of the cell 28 31577529
2017 EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions. Proceedings of the National Academy of Sciences of the United States of America 28 29162697
2015 Cytoplasmic and Nuclear Localizations Are Important for the Hypersensitive Response Conferred by Maize Autoactive Rp1-D21 Protein. Molecular plant-microbe interactions : MPMI 28 26039083
2009 Translation of intronless RNAs is strongly stimulated by the Epstein-Barr virus mRNA export factor EB2. Nucleic acids research 28 19528074
2005 End binding protein-1 (EB1) complements microtubule-associated protein-1B during axonogenesis. Journal of neuroscience research 28 15789376
1997 RP1, a new member of the adenomatous polyposis coli-binding EB1-like gene family, is differentially expressed in activated T cells. Journal of immunology (Baltimore, Md. : 1950) 28 9233623
2023 Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages. Nature communications 27 37704606
2019 Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends. eLife 27 31478831
2015 IGFBP-rP1 suppresses epithelial-mesenchymal transition and metastasis in colorectal cancer. Cell death & disease 27 25789970
2012 RP1 and retinitis pigmentosa: report of novel mutations and insight into mutational mechanism. The British journal of ophthalmology 27 22317909
2009 Probing interactions between CLIP-170, EB1, and microtubules. Journal of molecular biology 27 19913027
2018 EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26 29097501
2015 Regulation of Focal Adhesion Dynamics and Cell Motility by the EB2 and Hax1 Protein Complex. The Journal of biological chemistry 26 26527684
2014 γ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning. The EMBO journal 26 24421324
2014 End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis. FEBS letters 26 24492008
2018 Clinical significance of serum DRAM1 mRNA, ARSA mRNA, hsa-miR-2053 and lncRNA-RP1-86D1.3 axis expression in malignant pleural mesothelioma. Journal of cellular biochemistry 25 30362153
2007 The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules. Experimental cell research 25 17964570
2016 Interactions between the Microtubule Binding Protein EB1 and F-Actin. Journal of molecular biology 23 26854759
2019 EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22 31112396
2016 Proto-Oncogenic Src Phosphorylates EB1 to Regulate the Microtubule-Focal Adhesion Crosstalk and Stimulate Cell Migration. Theranostics 22 27698945
2025 RP1 Combined With Nivolumab in Advanced Anti-PD-1-Failed Melanoma (IGNYTE). Journal of clinical oncology : official journal of the American Society of Clinical Oncology 21 40627813
2020 WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. Frontiers in oncology 21 32426268
2019 The Location of Exon 4 Mutations in RP1 Raises Challenges for Genetic Counseling and Gene Therapy. American journal of ophthalmology 21 30731082
2019 EB1-3 Chain of IL-35 Along With TGF-β Synergistically Regulate Anti-leishmanial Immunity. Frontiers in immunology 21 31031744
2009 Structural basis of EB1 effects on microtubule dynamics. Biochemical Society transactions 21 19754439
2015 Autosomal recessive retinitis pigmentosa with RP1 mutations is associated with myopia. The British journal of ophthalmology 20 25883087
2010 Differential pattern of RP1 mutations in retinitis pigmentosa. Molecular vision 20 20664799
2002 A novel mutation of the RP1 gene (Lys778ter) associated with autosomal dominant retinitis pigmentosa. The British journal of ophthalmology 20 11864893
2004 Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barley. Molecular plant-microbe interactions : MPMI 18 15305606
2009 The APC-EB1 interaction. Advances in experimental medicine and biology 17 19928351