Affinage

EHD2

EH domain-containing protein 2 · UniProt Q9NZN4

Length
543 aa
Mass
61.2 kDa
Annotated
2026-04-28
49 papers in source corpus 25 papers cited in narrative 25 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EHD2 is a dynamin-related ATPase that stabilizes caveolae at the plasma membrane by forming ATP-dependent oligomeric filament scaffolds around caveolar necks, thereby restraining caveolar dynamics, endocytosis, and membrane trafficking. ATP binding triggers release of an autoinhibitory N-terminal segment, enabling membrane insertion and G-domain-mediated oligomerization into large ring-like assemblies that tether caveolae to the cortical actin network via PIP2-dependent targeting and interactions with PACSIN2 and cavin1; ATP hydrolysis drives membrane detachment and dynamic exchange at caveolae (PMID:22505029, PMID:28223496, PMID:24508342). By controlling caveolar stability, EHD2 regulates fatty acid uptake via CD36, Orai1-mediated store-operated calcium entry, eNOS-dependent NO production, Dll4/Notch signaling, KATP channel surface expression, and insulin-stimulated GLUT4 translocation (PMID:32170013, PMID:36625722, PMID:31600286, PMID:34820962, PMID:29133341, PMID:37703099). Under mechanical stress EHD2 is released from caveolae, SUMOylated, and translocated to the nucleus where it functions as a transcriptional repressor regulating genes including caveolae components and CDKN1A (PMID:30348749, PMID:22448906).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2003 High

    The first functional characterization of EHD2 established that its EH domain binds NPF motifs in EHBP1, that it localizes with cortical actin, and that loss of EHD2 or EHBP1 impairs clathrin-mediated endocytosis, placing EHD2 at the interface of endocytosis and actin remodeling.

    Evidence siRNA knockdown, Co-IP/pulldown, transferrin/GLUT4 endocytosis assays in cultured cells

    PMID:14676205

    Open questions at the time
    • Whether EHD2 acts directly on clathrin-coated pits versus caveolae was not distinguished
    • Mechanism of actin reorganization by EHD2 undefined
  2. 2004 Medium

    Identification of EHD2 on purified GLUT4 vesicles and demonstration that blocking EHD2 suppresses insulin-stimulated GLUT4 surface delivery established a role in regulated exocytic trafficking in adipocytes.

    Evidence MALDI-TOF MS, co-IP, antibody/peptide blockade in SLO-permeabilized rat adipocytes

    PMID:15182197

    Open questions at the time
    • Whether EHD2 acts on GLUT4 vesicle fusion or on caveolae-mediated surface retention was unresolved
    • Binding interface between EHD2 and GLUT4 vesicle components not mapped
  3. 2008 High

    Discovery of direct EHD2–myoferlin binding and the demonstration that dominant-negative EHD2 blocks myoblast fusion linked EHD2 to endocytic recycling and membrane fusion events during muscle differentiation.

    Evidence Direct binding assay, dominant-negative overexpression, transferrin recycling assay in myoblasts

    PMID:18502764

    Open questions at the time
    • Whether EHD2 delivers specific membrane cargo for fusion was not determined
    • Relative contribution of EHD2 versus EHD1 to recycling unclear
  4. 2012 High

    Three independent studies converged to redefine EHD2 as a caveolae-specific stabilizer: EHD2 associates with static plasma membrane caveolae via ATP-dependent oligomerization and interactions with PACSIN2/cavin1; its depletion increases caveolar mobility and endocytosis; and a parallel study revealed that EHD2 also shuttles to the nucleus upon SUMOylation to repress transcription.

    Evidence TIRF/live-cell imaging, siRNA KD, FRAP, sedimentation, Co-IP, NLS/NES/SUMO mutagenesis, GAL4 transactivation and qRT-PCR

    PMID:22323287 PMID:22448906 PMID:22505029

    Open questions at the time
    • Whether nuclear translocation is mechanically triggered was unknown
    • Structural basis of the autoinhibited-to-oligomeric transition not yet resolved
    • Direct transcriptional targets beyond CDKN1A not comprehensively identified
  5. 2014 High

    Crystal structure, cryo-EM, and EPR spectroscopy revealed that EHD2 inserts into membranes via helical-domain tip residues and that the N-terminal sequence is autoinhibitory in solution, released upon membrane binding, providing the structural basis for regulated membrane association.

    Evidence X-ray crystallography, cryo-EM, EPR, site-directed mutagenesis

    PMID:24508342

    Open questions at the time
    • Full oligomeric filament structure on membranes not resolved
    • Structural coupling between ATP hydrolysis and membrane detachment unknown
  6. 2017 High

    Biophysical reconstitution defined the complete ATPase-coupled membrane cycle: autoinhibition in solution → ATP-driven open conformation and membrane insertion → G-domain oligomerization on the membrane → ATP hydrolysis triggering detachment, explaining how EHD2 dynamically maintains caveolar stability.

    Evidence IRRAS, ATPase assay, mutagenesis, live-cell caveolae dynamics imaging

    PMID:28223496

    Open questions at the time
    • Whether hydrolysis occurs cooperatively within the oligomer was unresolved
    • Filament geometry on native caveolar necks not visualized
  7. 2018 High

    Mechanical stress was shown to trigger rapid EHD2 release from caveolae, SUMOylation, and nuclear translocation to regulate mechanoresponsive gene expression including caveolae components, establishing EHD2 as a mechanotransducer linking caveolar flattening to transcriptional programs.

    Evidence Live-cell imaging under stretch, SUMOylation assay, transcriptomics, metal-replica EM, KO/rescue in breast cancer cells

    PMID:30348749

    Open questions at the time
    • Identity of the SUMO E3 ligase acting on EHD2 unknown
    • Whether nuclear EHD2 binds DNA directly or via cofactors not defined
  8. 2019 High

    EHD2 global knockout mice revealed that caveolae stabilization by EHD2 controls whole-organism lipid metabolism (fatty acid uptake via CD36), endothelial eNOS/NO production, and vascular relaxation, establishing physiological consequences of caveolar destabilization in vivo.

    Evidence EHD2 KO mouse, fatty acid uptake assay, TIRF, electron microscopy, pressure myography, Ca2+ imaging, NO measurement

    PMID:31600286 PMID:32170013

    Open questions at the time
    • Whether phenotypes arise solely from increased caveolar endocytosis or also from altered signaling platform composition was unresolved
    • Compensatory mechanisms among EHD paralogs in vivo not characterized
  9. 2021 Medium

    EHD2 was shown to organize around Dll4 at caveolae in endothelial cells, and its knockout in zebrafish impaired Dll4 internalization and Notch signaling, revealing a role for EHD2-stabilized caveolae in vascular developmental signaling.

    Evidence Co-localization, caveolae disruption, EHD2 morpholino/KO in zebrafish, Notch reporter assay

    PMID:34820962

    Open questions at the time
    • Direct physical interaction between EHD2 and Dll4 not demonstrated
    • Whether EHD2 promotes or restricts Dll4 endocytosis is context-dependent and mechanistically unresolved
  10. 2023 Medium

    EHD2 was found to maintain Orai1 surface expression and store-operated calcium entry via caveolar stabilization, and separately to be required for insulin-stimulated GLUT4 translocation and SNARE complex formation in adipocytes, broadening the set of physiological processes controlled by EHD2-dependent caveolar homeostasis.

    Evidence CRISPR-Cas9 KO with rescue, Ca2+ imaging, Orai1 surface expression; EHD2 KO mice on high-fat diet, GLUT4 translocation assay, plasma membrane proteomics/lipidomics

    PMID:36625722 PMID:37703099

    Open questions at the time
    • Whether EHD2 directly interacts with Orai1 or acts indirectly via caveolae not resolved
    • How caveolar destabilization changes plasma membrane lipid composition mechanistically is unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: (1) the high-resolution structure of the EHD2 filament on native caveolar necks; (2) the identity of the SUMO ligase and chromatin targets mediating nuclear EHD2 transcriptional repression; and (3) how EHD2 coordinates with dynamin and other caveolar regulators to control the balance between caveolar stability and endocytic release.
  • In vivo filament architecture on caveolae at near-atomic resolution pending
  • Nuclear EHD2 transcriptional mechanism remains largely undefined
  • Functional interplay between EHD2 and dynamin at caveolae not dissected

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0140657 ATP-dependent activity 3 GO:0008289 lipid binding 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005886 plasma membrane 6 GO:0005634 nucleus 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-1430728 Metabolism 3 R-HSA-162582 Signal Transduction 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-9612973 Autophagy 1
Partners
CAV1CAVIN1EHBP1FER1L5MYOFPACSIN2RAB10
Complex memberships
Caveolae coat complex (with caveolin-1, cavin1, PACSIN2)

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 EHD2 contains an N-terminal P-loop and a C-terminal EH domain that interacts with NPF repeats in EHBP1; disruption of EHD2 or EHBP1 by siRNA inhibits transferrin and GLUT4 endocytosis into EEA1-positive endosomes; EHD2 localizes with cortical actin filaments and high expression causes extensive actin reorganization, linking clathrin-mediated endocytosis to the actin cytoskeleton. siRNA knockdown, Co-IP/pulldown, subcellular localization by fluorescence microscopy, endocytosis assays The Journal of biological chemistry High 14676205
2004 EHD2 is present in purified GLUT4 vesicles of rat adipocytes and co-immunoprecipitates with GLUT4; insulin treatment selectively enhances this interaction in exocytic vesicle fractions; antibody or peptide blockade of EHD2 suppresses insulin-induced plasma membrane GLUT4 recruitment by up to 75%. MALDI-TOF MS identification from purified GLUT4 vesicles, co-immunoprecipitation, GST pulldown, SLO-permeabilized adipocyte trafficking assay Biochemistry Medium 15182197
2008 EHD2 directly binds the second C2 domain of myoferlin; introduction of dominant-negative EHD2 sequesters myoferlin and inhibits myoblast fusion; myoferlin-null myoblasts accumulate transferrin and show delayed recycling, implicating EHD2 in endocytic recycling during myoblast membrane fusion. Direct binding assay (pulldown), dominant-negative overexpression, transferrin recycling assay, immunofluorescence The Journal of biological chemistry High 18502764
2010 EHD2 (and EHD1) bind directly to the second C2 domain of Fer1L5; reduction of EHD1 and/or EHD2 inhibits myoblast fusion; EHD2 is required for normal translocation of Fer1L5 to the plasma membrane. Direct binding assay, siRNA knockdown, myoblast fusion assay, immunofluorescence localization The Journal of biological chemistry Medium 21177873
2010 Prohibitin (PHB) undergoes palmitoylation at Cys69, which is required for its plasma membrane translocation; membrane-translocated PHB undergoes tyrosine phosphorylation and interacts with EHD2. Palmitoylation assay, site-directed mutagenesis (Cys69), co-immunoprecipitation, membrane fractionation Biochemistry and cell biology Medium 20555396
2011 EHD2 interacts with Nek3 kinase and Vav1 (a GEF for Rho GTPases) as identified by yeast two-hybrid; wild-type EHD2 (but not P-loop mutants) reduces Rac1 activity; inhibitory effect on Rac1 partially rescued by Rac1 co-expression, placing EHD2 upstream of Rac1 in trafficking from the plasma membrane. Yeast two-hybrid, Rac1 activity assay (G-LISA/pull-down), dominant-negative/P-loop mutant overexpression, cholera toxin trafficking assay The Biochemical journal Medium 21756249
2012 EHD2 is specifically and stably associated with caveolae at the plasma membrane (not clathrin-mediated endocytosis or endosomal recycling); EHD2 interacts with pacsin2 and cavin1; ordered membrane assembly requires cavin1 and caveolar integrity; a loop in the nucleotide-binding domain and ATP binding are required for caveolar localization; EHD2 stabilizes caveolae at the plasma membrane—its depletion results in more dynamic, short-lived, endocytic caveolae. Fluorescence microscopy, TIRF, siRNA knockdown, Co-IP, dominant-negative/mutant expression, live-cell imaging of caveolar dynamics Molecular biology of the cell High 22323287
2012 EHD2 (an ATPase) associates with the static population of plasma membrane caveolae; recruitment requires ATP binding, anionic lipid interaction, and oligomerization into large (60-75S) complexes via EH domain interactions with intrinsic NPF/KPF motifs; ATP hydrolysis is essential for binding to caveolae; EHD2 undergoes dynamic exchange at caveolae dependent on the ATPase cycle; depletion increases mobile caveolar vesicles; overexpression confines cholera toxin B in caveolae; confining role relies on linking caveolae to actin filaments. Sedimentation (sucrose gradient), siRNA knockdown, dominant-negative mutant expression, FRAP, TIRF live imaging, actin co-localization The EMBO journal High 22505029
2012 EHD2 shuttles to the nucleus via a nuclear localization signal (NLS); nuclear export depends partially on a nuclear export signal (NES); elimination of a SUMOylation site causes major nuclear accumulation and SUMOylation is confirmed by co-immunoprecipitation and yeast two-hybrid; nuclear EHD2 represses transcription including p21WAF1/Cip1 (CDKN1A) as shown by GAL4 transactivation and KLF7-dependent transcription assays, confirmed by qRT-PCR in KD and OE cells. Nuclear export inhibitor treatment, NLS/NES mutagenesis, co-immunoprecipitation, yeast two-hybrid, GAL4 transactivation assay, qRT-PCR The Biochemical journal High 22448906
2012 EHD2 accumulates at the site of laser-induced injury in human myotubes and at repair domes during sarcolemmal remodeling; EHD1 and a dominant-negative EHD2 mutant do not accumulate at injury sites, indicating a specific and ATPase-dependent role for EHD2 in sarcolemmal membrane repair. Live-cell laser injury assay, GFP-tagged protein localization, dominant-negative mutant expression, time-lapse fluorescence microscopy Traffic Medium 22679923
2013 Phosphatidylinositol 4,5-bisphosphate (PIP2) is required for EHD2 plasma membrane localization; pharmacologic perturbation of PIP2 metabolism redistributes EHD2 away from the plasma membrane; EHD2 colocalizes with PIP2-enriched Arf6-generated vacuoles; actin disruption by cytochalasin D co-redistributes both PIP2 and EHD2 to actin aggregates, indicating PIP2 rather than actin per se controls EHD2 localization. Confocal microscopy, pharmacologic PIP2 perturbation, Arf6 dominant-active expression, cytochalasin D treatment PloS one Medium 24040268
2014 EHD2 residues at the tip of the helical domain insert into the membrane (wedging mechanism creating curvature); the N terminus is folded into a hydrophobic pocket of the GTPase domain in solution and is released into the membrane upon membrane binding; cryo-EM shows N terminus is not essential for oligomerization but regulates targeting and stable association of EHD2 to caveolae. Electron paramagnetic resonance (EPR), X-ray crystallography, cryo-electron microscopy, site-directed mutagenesis Structure High 24508342
2015 The NPF motif of the EHD2 unstructured loop is required for homo-dimerization and Syndapin2 binding, whereas the KPF motif phenylalanine is essential for plasma membrane localization; NPF-to-NAF mutation abolishes dimerization and Syndapin2 binding but maintains plasma membrane association; NPF-to-APA mutation preserves dimerization and Syndapin2 binding but increases nuclear localization. Mutagenesis (NPF→NAF, NPF→APA), co-immunoprecipitation, confocal microscopy, nuclear localization quantification PloS one Medium 25875965
2016 A novel Rab10–EHBP1–EHD2 complex is essential for autophagic engulfment of lipid droplets (lipophagy) in hepatocytes; Rab10 activity increases during autophagy and recruits EHBP1 and EHD2 to nascent autophagic membranes at the lipid droplet surface; siRNA knockdown or GTPase-defective Rab10 leads to LD accumulation and impairs LC3 recruitment to the autophagosome. siRNA knockdown, GTPase-defective Rab10 expression, co-immunoprecipitation, immunofluorescence co-localization, autophagy assay Science advances High 28028537
2017 EHD2 is autoinhibited in solution via N-terminal residues and EH domain interactions; upon membrane binding EHD2 adopts an open conformation by tilting helical domains (shown by infrared reflection-absorption spectroscopy); ATP binding enables partial insertion into the membrane and G-domain-mediated oligomerization; ATP hydrolysis is coupled to detachment from the membrane; oligomerization in the membrane-bound state is required to restrict caveolae dynamics in cells. Infrared reflection-absorption spectroscopy (IRRAS), ATPase assay, mutagenesis, live-cell caveolae dynamics imaging PNAS High 28223496
2018 Under mechanical stress, EHD2 is rapidly released from caveolae, SUMOylated, and translocated to the nucleus where it regulates transcription of genes including caveolae constituents; EHD2 is required to maintain the caveolae reservoir during membrane tension variations; breast cancer cells lacking EHD2 show complete absence of caveolae and loss of mechanostress gene regulation, both rescued by EHD2 re-expression. Live-cell imaging of EHD2 under stretch, SUMOylation assay, nuclear fractionation, transcriptomics, metal-replica EM, EHD2 knockout/rescue in breast cancer cells The Journal of cell biology High 30348749
2018 EHD2 positively regulates surface expression of cardiac KATP channels by stabilizing caveolar structures and reducing endocytosis rate; this effect is specific to EHD2 (EHD1, EHD3, EHD4 have no effect); dominant-negative EHD2 sensitizes cardiomyocytes to ischemic damage without changing unitary conductance or ATP sensitivity of KATP channels. Immunofluorescence, surface biotinylation, patch clamping, dominant-negative expression, cardiomyocyte ischemia assay FASEB journal Medium 29133341
2019 EHD2 controls caveolae-dependent fatty acid uptake; EHD2 global knockout in mice increases lipid droplet size in fat tissue and fatty acid uptake via a caveolae- and CD36-dependent pathway involving dynamin; elevated numbers of detached caveolae are found in adipose tissue lacking EHD2, and caveolar mobility is increased in EHD2-null MEFs; EHD2 expression is down-regulated in visceral fat of obese mouse models and obese patients. EHD2 knockout mouse, fatty acid uptake assay, electron microscopy, TIRF live imaging of caveolae mobility, tissue fractionation PNAS High 32170013
2019 EHD2 controls caveolar dynamics to orchestrate eNOS activity; EHD2 deletion leads to increased detached caveolae in arteries, redistribution of eNOS from plasma membrane to internalized caveolae, decreased NO production, impaired mesenteric artery relaxation, and reduced cytosolic Ca2+ peaks in HUVECs after ATP stimulus. EHD2 knockout mouse, super-resolution imaging, NO measurement, pressure myography, Ca2+ imaging (HUVECs), eNOS immunolocalization PloS one High 31600286
2019 EHD2 protein expression is up-regulated at onset of adipocyte differentiation; siRNA-mediated EHD2 silencing impairs insulin sensitivity, lipid storage, and lipolysis; EHD2 localizes to caveolae near cell surface-associated lipid droplets; EHD2 overexpression increases lipolytic signaling and suppresses PPARγ transcription factor activity. siRNA knockdown, fluorescence imaging, insulin sensitivity assay, lipolysis assay, PPARγ activity assay, adipocyte differentiation model Molecular biology of the cell Medium 30811273
2021 EHD2 localizes to plasma membrane-bound Dll4 and caveolae in endothelial cells; disruption of caveolae prevents EHD2 organization around Dll4 and reduces Dll4 internalization; EHD2 knockout in zebrafish increases dysmorphic vascular sprouts and reduces downstream Notch signaling, identifying EHD2 as a modulator of Dll4 endocytosis and Notch activation during blood vessel development. In vitro co-localization, caveolae disruption, EHD2 morpholino/knockout in zebrafish, Notch reporter assay, in vivo vascular imaging Microcirculation Medium 34820962
2022 Super-resolution single-molecule localization shows PACSIN2 and EHD2 co-localize with caveolin-1 at typically sized caveolae; PACSIN2 F-BAR domain is positioned closer to the plasma membrane than EHD2 and caveolin-1, suggesting PACSIN2 connects caveolae to the plasma membrane while EHD2 is positioned more internally. 3D single-molecule localization super-resolution microscopy (STORM/PALM), geometric clustering analysis PloS one Medium 35834519
2023 EHD2-dependent stabilization of plasma membrane caveolae maintains high cell surface expression of the SOCE-linked calcium channel Orai1; EHD2 shRNA knockdown and CRISPR-Cas9 knockout reduce store-operated calcium entry (SOCE) and impair tumorigenesis and metastasis in TNBC cells, rescued by mouse Ehd2 re-expression. shRNA knockdown, CRISPR-Cas9 KO, mouse Ehd2 rescue, Ca2+ imaging (SOCE assay), Orai1 surface expression, tumorigenesis and metastasis assays eLife Medium 36625722
2023 EHD2 deficiency in adipocytes (EHD2 KO mice on high-fat diet and 3T3-L1 cells) is associated with deterioration of insulin signal transduction and impaired insulin-stimulated GLUT4 translocation; lack of EHD2 alters plasma membrane lipid and protein composition, reduces insulin receptor expression, and diminishes insulin-dependent SNARE protein complex formation. EHD2 KO mouse (high-fat diet), 3T3-L1 adipocyte siRNA KD, GLUT4 translocation assay, plasma membrane lipidomics/proteomics, insulin receptor expression, SNARE complex co-IP Molecular biology of the cell Medium 37703099
2025 Cryo-electron tomography of EHD2 filaments on tubulated liposomes shows EHD2 forms a highly curved membrane scaffold that stabilizes a tubular geometry with periodic undulations; the N-terminal sequence inserts into the membrane and acts as a spacer between adjacent filaments; in endothelial cells lacking EHD2, caveolar necks become narrower and elongated. Cryo-electron tomography, tubulated liposome reconstitution, N-terminal deletion mutagenesis, EM of EHD2-null endothelial caveolae bioRxiv (preprint)preprint High bio_10.1101_2025.06.05.658037

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 Ehd2, a rice ortholog of the maize INDETERMINATE1 gene, promotes flowering by up-regulating Ehd1. Plant physiology 198 18790997
2012 EHD2 regulates caveolar dynamics via ATP-driven targeting and oligomerization. Molecular biology of the cell 156 22323287
2016 A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Science advances 152 28028537
2012 Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin. The EMBO journal 135 22505029
2003 EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton. The Journal of biological chemistry 133 14676205
2008 The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. The Journal of biological chemistry 93 18502764
2000 EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins. Genomics 80 10673336
2009 EHD2 inhibits ligand-induced endocytosis and signaling of the leucine-rich repeat receptor-like protein LeEix2. The Plant journal : for cell and molecular biology 73 19392695
2018 EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription. The Journal of cell biology 61 30348749
2010 Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion. The Journal of biological chemistry 51 21177873
2020 EHD2-mediated restriction of caveolar dynamics regulates cellular fatty acid uptake. Proceedings of the National Academy of Sciences of the United States of America 49 32170013
2017 EHD2 restrains dynamics of caveolae by an ATP-dependent, membrane-bound, open conformation. Proceedings of the National Academy of Sciences of the United States of America 43 28223496
2014 Structural insights into membrane interaction and caveolar targeting of dynamin-like EHD2. Structure (London, England : 1993) 43 24508342
2012 Sarcolemmal repair is a slow process and includes EHD2. Traffic (Copenhagen, Denmark) 43 22679923
2009 The coiled-coil domain of EHD2 mediates inhibition of LeEix2 endocytosis and signaling. PloS one 31 19936242
2004 EHD2 interacts with the insulin-responsive glucose transporter (GLUT4) in rat adipocytes and may participate in insulin-induced GLUT4 recruitment. Biochemistry 31 15182197
2016 Phosphatidylinositol glycan anchor biosynthesis, class X containing complex promotes cancer cell proliferation through suppression of EHD2 and ZIC1, putative tumor suppressors. International journal of oncology 27 27572108
2013 Effects of EHD2 interference on migration of esophageal squamous cell carcinoma. Medical oncology (Northwood, London, England) 27 23354948
2011 EHD2 mediates trafficking from the plasma membrane by modulating Rac1 activity. The Biochemical journal 27 21756249
2013 Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization. PloS one 26 24040268
2010 Palmitoylation of prohibitin at cysteine 69 facilitates its membrane translocation and interaction with Eps 15 homology domain protein 2 (EHD2). Biochemistry and cell biology = Biochimie et biologie cellulaire 26 20555396
2012 EHD2 shuttles to the nucleus and represses transcription. The Biochemical journal 25 22448906
2015 Role of EHD2 in migration and invasion of human breast cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 23 25557791
2019 EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes. Molecular biology of the cell 21 30811273
2018 The trafficking protein, EHD2, positively regulates cardiac sarcolemmal KATP channel surface expression: role in cardioprotection. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21 29133341
2023 Exogenous abscisic acid represses rice flowering via SAPK8-ABF1-Ehd1/Ehd2 pathway. Journal of advanced research 19 37399924
2019 eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization. PloS one 16 31600286
2014 Upregulation of EHD2 after intracerebral hemorrhage in adult rats. Journal of molecular neuroscience : MN 15 24664435
2013 A point mutation in the zinc finger motif of RID1/EHD2/OsID1 protein leads to outstanding yield-related traits in japonica rice variety Wuyunjing 7. Rice (New York, N.Y.) 14 24280027
2013 Scratching the surface: actin' and other roles for the C-terminal Eps15 homology domain protein, EHD2. Histology and histopathology 14 24347515
2020 TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2. International journal of molecular medicine 11 32945345
2023 EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry. eLife 10 36625722
2009 EHD2 inhibits signaling of leucine rich repeat receptor-like proteins. Plant signaling & behavior 10 19820301
2021 EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer. Cancer investigation 7 33356637
2021 Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B. American journal of cancer research 7 33520362
2015 Role of the EHD2 unstructured loop in dimerization, protein binding and subcellular localization. PloS one 7 25875965
2013 The function of EHD2 in endocytosis and defense signaling is affected by SUMO. Plant molecular biology 7 24154852
2022 Differential requirements for the Eps15 homology domain proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic (Copenhagen, Denmark) 6 35510564
2022 Super-resolution analysis of PACSIN2 and EHD2 at caveolae. PloS one 6 35834519
2021 EHD2 modulates Dll4 endocytosis during blood vessel development. Microcirculation (New York, N.Y. : 1994) 6 34820962
2020 EHD2 is a Predictive Biomarker of Chemotherapy Efficacy in Triple Negative Breast Carcinoma. Scientific reports 4 32409676
2024 OsCOL5 suppresses heading through modulation of Ghd7 and Ehd2, enhancing rice yield. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 3 38884792
2023 EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events. Molecular biology of the cell 3 37703099
2022 qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression. Plant science : an international journal of experimental plant biology 3 36041562
2023 Novel MYCBP::EHD2 and RUNX1::ZNF780A Fusion Genes in T-cell Acute Lymphoblastic Leukemia. Cancer genomics & proteomics 2 36581344
2024 EH domain-containing protein 2 (EHD2): Overview, biological function, and therapeutic potential. Cell biochemistry and function 1 38613224
2025 A Series of Novel Alleles of Ehd2 Modulating Heading and Salt Tolerance in Rice. Plants (Basel, Switzerland) 0 39861650
2023 EHD2, a novel HIF target gene, is a promising biomarker in clear cell renal cell carcinoma. International journal of clinical and experimental pathology 0 38059172
2020 [Unsaturated fatty acid of Actinidia chinesis planch seed oil (kiwi fruit essence) inhibits growth and metastasis of transplanted tumor in lung adenocarcinoma mice by up-regulating EHD2 expression]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 0 32727647