Affinage

Showing CDC5L — CEF1 is a alias.

CDC5L

Cell division cycle 5-like protein · UniProt Q99459

Length
802 aa
Mass
92.3 kDa
Annotated
2026-06-09
100 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDC5L is a core component of the human Prp19/CDC5L spliceosomal complex and is required for the second catalytic step of pre-mRNA splicing; it incorporates into the spliceosome in an ATP-dependent step and associates with the spliceosome throughout the reaction, with immunodepletion blocking splicing product formation without preventing spliceosome assembly (PMID:11101529, PMID:10570151). Within the complex it forms a stable core with hPrp19, PRL1, and SPF27, in which SPF27 contacts each core subunit and a protease-resistant subcomplex includes the C-terminus of CDC5L (PMID:20176811). CDC5L makes a direct, splicing-essential interaction with PLRG1 through its C-terminal region binding the PLRG1 WD40 domain, and competing peptides that disrupt this interface abolish splicing in vitro (PMID:11544257, PMID:14576297). Its incorporation and complex integrity are mediated by CTNNBL1, which binds the CDC5L nuclear localization sequence via its armadillo domain and functions as a chaperone that supports CWC15-CDC5L association and overall Prp19 complex levels (PMID:21385873, PMID:26130721). Splicing activity is gated by cell-cycle phosphorylation: CDK2 phosphorylates CDC5L at T411/T438, an event required for splicing, while NIPP1/PP1 docks onto cyclin E-Cdk2-phosphorylated CDC5L through its FHA domain (PMID:18583928, PMID:10827081). Beyond splicing, CDC5L physically associates with the checkpoint kinase ATR and is required for ATR-dependent S-phase checkpoint signaling, including phosphorylation of Chk1, Rad17, and FancD2 (PMID:19633697). Loss of CDC5L causes mitotic catastrophe with chromosome misalignment, impaired kinetochore-microtubule attachment, and defective splicing of mitosis and DNA-damage-response genes (PMID:24675469), and in chondrocytes CDC5L directly binds and regulates splicing of SOX9, COL2A1, and WEE1 to drive chondrogenesis (PMID:34298017). The N-terminal Myb-like repeats confer site-specific DNA binding and transcriptional activation activity (PMID:11082045).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1998 Medium

    Established CDC5L as a nuclear ~100 kDa protein with N-terminal Myb-related DNA-binding domain and a central proline-rich activation domain, and showed overexpression promotes G2/M progression — first hint of a cell-cycle role.

    Evidence Genomic organization mapping and overexpression/dominant-negative cell-cycle analysis in mammalian cells

    PMID:9468527 PMID:9598309

    Open questions at the time
    • G2/M effect inferred from over/under-expression without endogenous pathway placement
    • Molecular function (splicing) not yet defined
  2. 1999 High

    Defined CDC5L as a spliceosome-associated factor conserved from yeast, addressing whether the Myb protein had an RNA-processing function.

    Evidence Immunofluorescence colocalization with splicing factors, nuclear-extract co-IP, in vitro spliceosome assembly, and yeast CEF1 depletion

    PMID:10570151

    Open questions at the time
    • Whether CDC5L acts catalytically or structurally not resolved
    • Step of splicing affected in human system unclear
  3. 2000 High

    Demonstrated that the CDC5L complex is required specifically for the second catalytic step of splicing and joins the spliceosome in an ATP-dependent manner, distinguishing its role from assembly.

    Evidence Immunodepletion/add-back from HeLa nuclear extract with in vitro splicing and MS identification of complex members

    PMID:11101529

    Open questions at the time
    • Individual subunit contributions not yet dissected
    • Mechanism of catalytic-step facilitation unknown
  4. 2000 High

    Linked CDC5L splicing activity to cell-cycle kinase signaling by showing cyclin E-Cdk2 phosphorylation creates a docking site for the NIPP1/PP1 FHA module.

    Evidence Yeast two-hybrid, reciprocal co-IP, nuclear-extract co-purification, and FHA-domain mutant splicing assays

    PMID:10827081

    Open questions at the time
    • Precise phosphosites not mapped here
    • How PP1 activity modulates splicing kinetics unresolved
  5. 2001 High

    Identified the CDC5L-PLRG1 interaction (C-terminus to WD40 domain) as a direct, mechanistically essential contact for splicing.

    Evidence Co-IP, direct in vitro binding, and competitive peptide disruption in in vitro splicing

    PMID:11544257

    Open questions at the time
    • Structural basis of the interface not resolved
    • Function of PLRG1 within the catalytic step unclear
  6. 2003 Medium

    Mapped additional spliceosomal interactions (hLodestar/HuF2) and confirmed CDC5L-PLRG1 essentiality, extending the interaction network required for splicing.

    Evidence Yeast two-hybrid, co-IP, and competitive peptide/inhibition in vitro splicing assays

    PMID:12927788 PMID:14576297

    Open questions at the time
    • Role of hLodestar in assembly versus catalysis not separated
    • In vivo relevance of these contacts limited
  7. 2008 High

    Pinpointed CDK2 phosphorylation of CDC5L at T411/T438 as required for splicing, mechanistically connecting cell-cycle kinases to spliceosome function.

    Evidence Phosphopeptide mapping, MS, in vitro kinase assay with CDK2 inhibitor, and splicing assays with phosphosite mutants

    PMID:18583928

    Open questions at the time
    • How phosphorylation alters complex behavior structurally unknown
    • Homodimerization function not defined
  8. 2009 High

    Revealed a splicing-independent role: CDC5L binds ATR and is required for S-phase checkpoint activation, broadening its function into the DNA-replication stress response.

    Evidence Co-IP, RNAi knockdown, Chk1/Rad17/FancD2 phosphorylation assays, and ATR-binding-deficient deletion-mutant rescue

    PMID:19633697

    Open questions at the time
    • Direct biochemical role of CDC5L in ATR activation unresolved
    • Whether checkpoint role depends on the splicing complex unclear
  9. 2010 High

    Resolved the architecture of the human Prp19/CDC5L complex, defining a stable CDC5L/hPrp19/PRL1/SPF27 core, and characterized regulatory interactions including hnRNP-M and Akt-dependent PRP19α/14-3-3β assembly.

    Evidence Native complex purification, stoichiometry, limited proteolysis and EM; co-IP and domain-mapping for hnRNP-M and PRP19α phospho-dependent assembly

    PMID:20176811 PMID:20467437 PMID:20629186

    Open questions at the time
    • Atomic structure of the complex not determined
    • How accessory factors remodel the core during the cycle unknown
  10. 2011 Medium

    Showed CTNNBL1 binds the CDC5L NLS via its armadillo domain to integrate CDC5L into the Prp19 complex, identifying a recruitment/chaperone mechanism.

    Evidence Co-IP, domain mapping, and NLS-binding assays

    PMID:21385873

    Open questions at the time
    • Whether CTNNBL1 affects nuclear import versus complex assembly not separated
  11. 2014 Medium

    Connected CDC5L splicing function to mitotic fidelity, showing depletion causes mitotic catastrophe through impaired splicing of mitosis and DNA-damage genes.

    Evidence RNAi, live-cell imaging, chromosome spreads, genome-wide expression and splicing-efficiency profiling

    PMID:24675469

    Open questions at the time
    • Which specific splicing targets drive each phenotype not fully resolved
    • Direct versus indirect transcript effects not separated
  12. 2015 Medium

    Defined CTNNBL1 as a chaperone required for Prp19 complex integrity, showing it promotes CWC15-CDC5L association via an overlapping binding region.

    Evidence Crosslinking + HDX-MS, in vitro binding, and CTNNBL1-deficient cell analysis

    PMID:26130721

    Open questions at the time
    • Dynamics of CWC15/CTNNBL1 exchange on CDC5L not directly observed
  13. 2021 Medium

    Demonstrated tissue-specific, target-directed splicing by CDC5L, which directly binds and regulates SOX9, COL2A1, and WEE1 transcripts to drive chondrogenesis.

    Evidence siRNA, RNA-binding protein immunoprecipitation, splicing-efficiency assays, and cartilage rudiment culture

    PMID:34298017

    Open questions at the time
    • Sequence determinants of CDC5L transcript selectivity unknown
    • Whether direct RNA contact is via Myb-like domain not established here
  14. 2017 Low

    Reported direct CDC5L promoter binding and transcriptional activation activity at hTERT and other genes, supporting a DNA-binding/transcription function distinct from splicing.

    Evidence DNA pulldown, ChIP, luciferase reporter, and siRNA assays in tumor cells

    PMID:11082045 PMID:28472785 PMID:32167655

    Open questions at the time
    • Transcriptional role demonstrated in limited cell contexts without endogenous-promoter mechanism
    • Relationship between DNA-binding and spliceosomal pools unclear
  15. 2024 Low

    Defined upstream regulation of CDC5L abundance by Prp19 (post-transcriptional/lysosomal) and by the m6A reader IGF2BP1, linking CDC5L levels to proliferation in disease contexts.

    Evidence siRNA/overexpression rescue, lysosomal inhibitors, m6A-seq, and RIP in HCC and myeloma cells

    PMID:28387715 PMID:39534570

    Open questions at the time
    • Mechanisms inferred without direct biochemical reconstitution
    • Generality beyond specific cancer models untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CDC5L's distinct activities — spliceosomal catalysis, ATR checkpoint signaling, and Myb-mediated DNA binding/transcription — are coordinated and whether they reflect physically separate protein pools remains unresolved.
  • No structure of the human complex bound to spliceosome substrate
  • Mechanism integrating phosphorylation, complex assembly, and non-splicing roles unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 2 GO:0140098 catalytic activity, acting on RNA 2 GO:0003677 DNA binding 1 GO:0005198 structural molecule activity 1 GO:0140110 transcription regulator activity 1
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 2
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-1640170 Cell Cycle 2 R-HSA-73894 DNA Repair 1
Partners
ATRBCAS2CTNNBL1CWC15HNRNPMNIPP1PLRG1PRPF19
Complex memberships
Prp19/CDC5L complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 CDC5L is part of a larger multiprotein complex in HeLa nuclear extract that incorporates into the spliceosome in an ATP-dependent step and is required for the second catalytic step of pre-mRNA splicing. Immunodepletion of the CDC5L complex inhibits pre-mRNA splicing product formation in vitro but does not prevent spliceosome assembly; the purified complex restores splicing activity when added back to immunodepleted extracts. Immunodepletion from HeLa nuclear extract, in vitro splicing assay, mass spectrometry identification of complex components The EMBO journal High 11101529
2001 CDC5L directly interacts with PLRG1 via the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1; disruption of this interaction by a bacterially expressed competing peptide inhibits pre-mRNA splicing in HeLa nuclear extract, demonstrating that this protein-protein interaction is essential for pre-mRNA splicing. Co-immunoprecipitation in vivo, direct in vitro binding assay, competitive peptide disruption with in vitro splicing assay The Journal of biological chemistry High 11544257
2010 The human Prp19/CDC5L complex contains four copies of hPrp19, with a stable core comprised of CDC5L, hPrp19, PRL1, and SPF27. SPF27 directly interacts with each core component; limited proteolysis revealed a protease-resistant sub-complex of SPF27, the C-terminus of CDC5L, and N-termini of PRL1 and hPrp19. Under EM the complex has an elongated asymmetric shape (~20 nm). Native complex purification from HeLa cells, stoichiometric analysis, salt-treatment dissection, protein-protein interaction studies, limited proteolysis, electron microscopy Molecular and cellular biology High 20176811
2000 NIPP1, a regulatory subunit of protein phosphatase-1, interacts with CDC5L through its FHA (forkhead-associated) domain in a phosphorylation-dependent manner; CDC5L is phosphorylated by cyclin E-Cdk2, which is required for the NIPP1 FHA domain to bind CDC5L. CDC5L, NIPP1, and PP1 form a complex in rat liver nuclear extracts. Expression of the NIPP1 FHA domain in cells blocks beta-globin pre-mRNA splicing, and a mutation that abolishes FHA-CDC5L interaction also abolishes this anti-splicing effect. Yeast two-hybrid, co-immunoprecipitation, co-purification from nuclear extracts, in vitro splicing assay, FHA domain mutant analysis The Journal of biological chemistry High 10827081
1999 CDC5 (mammalian) colocalizes with pre-mRNA splicing factors in mammalian nuclei, associates with core spliceosomal components in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Genetic depletion of the yeast homolog CEF1 blocks the first step of pre-mRNA processing in vivo. Immunofluorescence colocalization, nuclear extract co-immunoprecipitation, in vitro spliceosome assembly, yeast genetic depletion Proceedings of the National Academy of Sciences of the United States of America High 10570151
2009 CDC5L physically interacts with the checkpoint kinase ATR; depletion of CDC5L by RNAi causes a defective S-phase cell-cycle checkpoint and cellular sensitivity to replication-fork blocking agents. CDC5L is required for activation of downstream ATR effectors Chk1, Rad17, and FancD2. A CDC5L deletion mutant unable to bind ATR fails to rescue the checkpoint deficiency in CDC5L-depleted cells. Co-immunoprecipitation, RNAi knockdown, checkpoint assays (Chk1/Rad17/FancD2 phosphorylation), domain-mapping with deletion mutant rescue EMBO reports High 19633697
2008 Cell cycle-dependent phosphorylation of human CDC5L at threonines 411 and 438 (within CDK recognition sequences) is required for CDC5L-mediated pre-mRNA splicing in vitro. CDK2 phosphorylates CDC5L in vitro and in vivo; a specific CDK2 inhibitor (CVT-313) inhibits CDC5L phosphorylation. CDC5L forms homodimers in vitro and in vivo, but homodimerization and nuclear localization do not depend on phosphorylation at these sites. 2D phosphopeptide mapping, nanoelectrospray mass spectrometry, in vitro splicing assay with phosphorylation-site mutants, in vitro kinase assay, in vivo radiolabeling Cell cycle (Georgetown, Tex.) High 18583928
1998 Human CDC5 (hCdc5/CDC5L) promotes G2/M progression in mammalian cells: overexpression shortened G2 and reduced cell size, while a dominant-negative mutant lacking the C-terminal activation domain slowed G2 progression and delayed mitotic entry. Overexpression and dominant-negative mutant analysis in mammalian cells, cell cycle analysis The Journal of biological chemistry Medium 9468527
2000 Human CDC5 binds specifically and with high affinity to a 12 bp DNA sequence through its amino terminus, and this DNA-protein interaction is capable of activating transcription. Multiple human genomic sequences with similar motifs also interact with CDC5. DNA binding assay, transcriptional activation assay, yeast selection system for genomic binding sites Journal of cell science Medium 11082045
2010 hnRNP-M directly interacts with CDC5L and PLRG1 in vivo; this interaction is inhibited during heat-shock stress. A central region of hnRNP-M is required for CDC5L/PLRG1 interaction, and an hnRNP-M mutant lacking this domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate. In vivo interaction assays, domain-mapping with truncation mutants, in vitro/in vivo alternative splicing assays EMBO reports Medium 20467437
2011 CTNNBL1 binds the nuclear localization sequence (NLS) of CDC5L via its armadillo domain, mediating CTNNBL1 association with the Prp19 spliceosomal complex. CTNNBL1 also interacts with Prp31 through its NLS, but via a binding specificity distinct from karyopherin α. Co-immunoprecipitation, domain-mapping, NLS-binding assays The Journal of biological chemistry Medium 21385873
2015 CTNNBL1 enhances the association of CWC15 with CDC5L in vitro; in vivo, CTNNBL1 deficiency reduces normal levels of the Prp19 complex and impairs CWC15-CDC5L interaction. The region of CDC5L that binds CTNNBL1 overlaps with that which binds CWC15, suggesting the two proteins may exchange at the complex. CTNNBL1 thus has a chaperone function required for Prp19 complex integrity. Amine crosslinking + hydrogen-deuterium exchange MS, in vitro binding assays, in vivo complex abundance analysis, CTNNBL1-deficient cells Nucleic acids research Medium 26130721
2003 Peptides derived from the CDC5L-PLRG1 binding interface inhibit pre-mRNA splicing in vitro; this inhibition is prevented by pre-incubating peptides with the corresponding partner protein, confirming that the direct CDC5L-PLRG1 interaction is mechanistically essential for splicing. Competitive peptide inhibition in in vitro splicing assay, rescue by recombinant partner protein Nucleic acids research Medium 14576297
2002 DAP-like kinase (Dlk/ZIP kinase) interacts with rat CDC5 (homolog of human CDC5L) in vitro, but does not phosphorylate it directly; instead, an associated kinase identified as CK2 phosphorylates CDC5. Both proteins co-localize in nuclear speckles in vivo. The interaction domain of Dlk maps to its leucine zipper, and that of CDC5 maps to its C-terminal region (residues 500-802). In vitro binding assay, in vitro kinase assay, immunofluorescence colocalization, domain mapping Nucleic acids research Medium 11884640
2003 hLodestar/HuF2 (SNF2 family) interacts with CDC5L in yeast two-hybrid and HeLa nuclear extracts; a truncated hLodestar/HuF2 polypeptide overlapping the CDC5L-binding region inhibits pre-mRNA splicing by disrupting spliceosome assembly. Yeast two-hybrid, co-immunoprecipitation from HeLa nuclear extract, in vitro splicing inhibition assay Biochemical and biophysical research communications Medium 12927788
2012 Alleles of yeast CEF1 (the S. cerevisiae ortholog of CDC5L) suppress second-step splicing defects caused by intron mutations, U6 snRNA mutations, or deletion of the second-step factor Prp17, and can activate alternative 3' splice sites. Genetic interactions with prp8 alleles suggest CEF1/CDC5L modulates the first-to-second-step conformational transition of the spliceosome, likely through its Myb-like domain. Genetic suppressor analysis, in vitro splicing assays with mutant alleles, epistasis with prp8 and U6 alleles RNA (New York, N.Y.) Medium 22408182
2014 The N-terminus of S. pombe Cdc5 (CDC5L ortholog) contains two canonical Myb repeats (R1, R2) and a third domain (D3) that, while not adopting a canonical Myb fold, preferentially binds double-stranded RNA in vitro. All three domains (R1, R2, D3) are required for Cdc5 function in yeast cells. Yeast genetics (truncation mutants), NMR/structural analysis of D3, RNA binding assays (EMSA), functional complementation Biochemistry Medium 25263959
2010 Akt phosphorylates PRP19α at Thr193, which is critical for PRP19α binding to 14-3-3β; this allows nuclear translocation and formation of a PRP19α/14-3-3β/CDC5L complex required for active spliceosome assembly during NGF-induced neuronal differentiation of PC12 cells. A nonphosphorylatable PRP19α T193A mutant loses 14-3-3β binding and acts as a dominant negative in neuronal differentiation. Co-immunoprecipitation, dominant-negative mutant analysis, knockdown/overexpression in PC12 cells, nuclear fractionation Journal of neuroscience research Medium 20629186
2003 Human CDC5 (CDC5L) nuclear import is directed by the amino-terminal domain independent of consensus nuclear localization signals or phosphorylation, while the carboxyl-terminus preferentially associates with spliceosomal complexes near RNA transcription sites during interphase. CDC5L colocalizes with Sm proteins in a cell cycle- and domain-dependent manner. Domain-deletion constructs, nuclear fractionation, immunofluorescence colocalization, cell cycle analysis Cell biochemistry and biophysics Medium 14515018
2014 Depletion of CDC5L causes dramatic mitotic arrest, chromosome misalignments, sustained spindle assembly checkpoint activation, severe impairment of kinetochore-microtubule attachment, and DNA damage, ultimately leading to mitotic catastrophe. Genome-wide expression analysis reveals that CDC5L modulates expression of mitosis and DNA damage response genes, and pre-mRNA splicing efficiency of these genes is impaired upon CDC5L knockdown. RNAi knockdown, live-cell imaging, chromosome spread analysis, genome-wide expression profiling, splicing efficiency assays Cell death & disease Medium 24675469
2021 Cdc5l (CDC5L) promotes early chondrogenesis and chondrocyte proliferation by modulating pre-mRNA splicing of Sox9, Col2a1, and Wee1. Knockdown of Cdc5l in murine chondrocytes decreased Sox9 and Col2a1 expression, enhanced Wee1 expression (causing G2/M arrest), and reduced pre-mRNA splicing efficiency of Sox9 and Col2a1 while paradoxically enhancing splicing of Wee1 pre-mRNA. RNA-binding protein immunoprecipitation confirmed direct binding of Cdc5l to these target transcripts. siRNA knockdown, FACS cell cycle analysis, cartilage rudiment culture, RNA-binding protein immunoprecipitation (RIP), splicing efficiency assays The Journal of biological chemistry Medium 34298017
1998 The human CDC5L gene maps to chromosome 6p21, consists of at least 16 exons spanning ~50 kb, and encodes a ~100 kDa nuclear protein. Immunocytochemistry confirmed nuclear localization. The protein contains a Myb-related DNA binding domain and nuclear localization signals in its N-terminus and a proline-rich putative transcriptional activating domain in its central region. Genomic organization mapping, immunocytochemistry, Western blot, Northern blot Genomics Low 9598309
1997 Human CDC5L (PCDC5RP) translocates rapidly from cytoplasm to nucleus upon serum stimulation of cultured cells, correlating temporally with increased CDC5L phosphorylation, suggesting it transduces cytoplasmic signals to the nucleus. Immunofluorescence, subcellular fractionation, phosphorylation analysis after serum stimulation The Journal of biological chemistry Low 9038199
2017 CDC5L binds directly to the hTERT promoter (identified by pulldown with biotin-labeled hTERT promoter and confirmed by ChIP assay) and acts as a transcriptional activator of hTERT, as shown by luciferase reporter assay. CDC5L knockdown inhibits tumor growth by down-regulating hTERT expression. Biotin-labeled DNA pulldown, ChIP assay, luciferase reporter assay, siRNA knockdown Cellular physiology and biochemistry Low 28472785
2020 CDC5L protein binds directly to the PEAK1 gene promoter to promote its transcription, as confirmed by chromatin immunoprecipitation (ChIP) assay in ovarian cancer cells. ChIP assay, transcriptional reporter, siRNA knockdown Cancer medicine Low 32167655
2017 Prp19 regulates CDC5L protein levels post-transcriptionally: silencing Prp19 in HCC cells inhibits Cdc5L mRNA translation and facilitates lysosome-mediated degradation of CDC5L protein. Overexpression of CDC5L partially rescues the cell cycle arrest caused by Prp19 knockdown, placing Prp19 upstream of CDC5L in the mitotic progression pathway. siRNA knockdown, overexpression rescue, lysosomal inhibitor experiments, Western blot, flow cytometry International journal of molecular sciences Low 28387715
2024 IGF2BP1, an m6A reader, binds to m6A sites on CDC5L mRNA and up-regulates CDC5L protein abundance post-transcriptionally. Knockdown or mutation of CDC5L attenuates the pro-proliferative effect of IGF2BP1 in multiple myeloma cells with chromosome 1q gain. m6A sequencing, RIP assay, siRNA knockdown, mutant analysis, in vitro and in vivo proliferation assays Genes & diseases Low 39534570

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 CDC5 and CKII control adaptation to the yeast DNA damage checkpoint. Cell 369 9323137
2001 Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast. Cell 313 11371343
1994 Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5. Journal of cell science 260 7962193
1993 A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Molecular and cellular biology 237 8321244
2001 Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 225 11733064
1998 The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae. Current biology : CB 217 9560342
2003 Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation. Science (New York, N.Y.) 208 12663816
2003 Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I. Nature cell biology 182 12717442
2000 Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. The EMBO journal 179 11101529
1997 Plk is a functional homolog of Saccharomyces cerevisiae Cdc5, and elevated Plk activity induces multiple septation structures. Molecular and cellular biology 166 9154840
2008 Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis. Genes & development 150 18832066
2000 Essential function of the polo box of Cdc5 in subcellular localization and induction of cytokinetic structures. Molecular and cellular biology 141 10594031
2001 Order of function of the budding-yeast mitotic exit-network proteins Tem1, Cdc15, Mob1, Dbf2, and Cdc5. Current biology : CB 133 11378390
2006 Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science (New York, N.Y.) 129 16763112
2013 CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 119 24101471
2013 Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover. The EMBO journal 115 23531881
2010 Molecular architecture of the human Prp19/CDC5L complex. Molecular and cellular biology 113 20176811
2001 A novel function of Saccharomyces cerevisiae CDC5 in cytokinesis. The Journal of cell biology 110 11157974
2000 NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry. The Journal of biological chemistry 95 10827081
1994 The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. The EMBO journal 86 8313892
2009 Cdc5L interacts with ATR and is required for the S-phase cell-cycle checkpoint. EMBO reports 83 19633697
2018 Oncogenic Properties of NEAT1 in Prostate Cancer Cells Depend on the CDC5L-AGRN Transcriptional Regulation Circuit. Cancer research 82 29871935
2003 The role of the polo kinase Cdc5 in controlling Cdc14 localization. Molecular biology of the cell 82 14551257
2003 In vitro regulation of budding yeast Bfa1/Bub2 GAP activity by Cdc5. The Journal of biological chemistry 81 12637549
2008 APC/C-Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus. Genes & development 75 18172166
2008 Cell cycle regulator gene CDC5L, a potential target for 6p12-p21 amplicon in osteosarcoma. Molecular cancer research : MCR 74 18567798
1999 Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proceedings of the National Academy of Sciences of the United States of America 69 10570151
2014 Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe. Cell death & disease 65 24675469
2002 Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC molecular biology 63 11960554
2005 Cdc28-dependent regulation of the Cdc5/Polo kinase. Current biology : CB 60 16303563
2010 Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice. EMBO reports 59 20467437
1998 A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry. The Journal of biological chemistry 59 9468527
2002 Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus. Biochemical and biophysical research communications 57 12056824
2015 CEF1/OsMYB103L is involved in GA-mediated regulation of secondary wall biosynthesis in rice. Plant molecular biology 52 26350403
2013 Polo kinase Cdc5 is a central regulator of meiosis I. Proceedings of the National Academy of Sciences of the United States of America 50 23918381
2014 Digital expression profiling identifies RUNX2, CDC5L, MDM2, RECQL4, and CDK4 as potential predictive biomarkers for neo-adjuvant chemotherapy response in paediatric osteosarcoma. PloS one 49 24835790
1997 Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling. The Journal of biological chemistry 49 9038199
2010 CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation. PLoS biology 48 20126259
2001 A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing. The Journal of biological chemistry 48 11544257
2001 Cdc5 interacts with the Wee1 kinase in budding yeast. Molecular and cellular biology 45 11438652
2020 Depletion of CDC5L inhibits bladder cancer tumorigenesis. Journal of Cancer 42 31897231
2010 Elevated levels of the polo kinase Cdc5 override the Mec1/ATR checkpoint in budding yeast by acting at different steps of the signaling pathway. PLoS genetics 41 20098491
2000 Human Cdc5, a regulator of mitotic entry, can act as a site-specific DNA binding protein. Journal of cell science 38 11082045
2020 Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway. Cancer medicine 37 32167655
2012 α-Synuclein disrupts stress signaling by inhibiting polo-like kinase Cdc5/Plk2. Proceedings of the National Academy of Sciences of the United States of America 36 22988096
2003 Loss of CDC5 function in Saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics 36 12586693
2008 Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics. Eukaryotic cell 35 18178775
2020 Exo1 recruits Cdc5 polo kinase to MutLγ to ensure efficient meiotic crossover formation. Proceedings of the National Academy of Sciences of the United States of America 34 33199619
2011 CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31. The Journal of biological chemistry 34 21385873
2010 Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction. The Journal of biological chemistry 33 21036905
2014 The yeast polo kinase Cdc5 regulates the shape of the mitotic nucleus. Current biology : CB 32 25454593
2017 Functions and regulation of the Polo-like kinase Cdc5 in the absence and presence of DNA damage. Current genetics 31 28770345
1998 Rearrangement of the human CDC5L gene by a t(6;19)(p21;q13.1) in a patient with multicystic renal dysplasia. Genomics 30 9598309
2009 The yeast SUMO isopeptidase Smt4/Ulp2 and the polo kinase Cdc5 act in an opposing fashion to regulate sumoylation in mitosis and cohesion at centromeres. Cell cycle (Georgetown, Tex.) 29 19823017
2017 CDC5L Promotes hTERT Expression and Colorectal Tumor Growth. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 27 28472785
2015 Expression and Clinical Role of Cdc5L as a Novel Cell Cycle Protein in Hepatocellular Carcinoma. Digestive diseases and sciences 26 26553251
2011 The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway. Molecular biology of the cell 26 21795394
2011 Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability. Proceedings of the National Academy of Sciences of the United States of America 26 21987786
2010 Cell cycle-dependent phosphorylation of Rad53 kinase by Cdc5 and Cdc28 modulates checkpoint adaptation. Cell cycle (Georgetown, Tex.) 26 20046099
2008 Cell cycle-dependent phosphorylation of human CDC5 regulates RNA processing. Cell cycle (Georgetown, Tex.) 24 18583928
2016 Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1. Cell reports 23 27210759
2015 Expression of CDC5L is associated with tumor progression in gliomas. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 23 26490980
2008 The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell cycle (Georgetown, Tex.) 23 18927509
2019 Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast. Molecular biology of the cell 21 30726152
2017 Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells. International journal of molecular sciences 21 28387715
2012 Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit. PLoS genetics 21 22253605
2009 The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase. Molecular biology of the cell 21 19570916
2003 Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1. Nucleic acids research 21 14576297
2016 Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis. Molecular biology of the cell 19 27226485
2002 DAP-like kinase interacts with the rat homolog of Schizosaccharomyces pombe CDC5 protein, a factor involved in pre-mRNA splicing and required for G2/M phase transition. Nucleic acids research 19 11884640
2015 CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic acids research 18 26130721
2016 Centrosome-Dependent Bypass of the DNA Damage Checkpoint by the Polo Kinase Cdc5. Cell reports 17 26832404
2014 The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit. Cell cycle (Georgetown, Tex.) 17 25485506
2012 CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA (New York, N.Y.) 17 22408182
2021 Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L). Bioengineered 16 34903139
2020 ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L. Aging 16 32526706
2020 Long Noncoding RNA LINC00963 Promotes CDC5L-Mediated Malignant Progression in Gastric Cancer. OncoTargets and therapy 16 33376349
1981 Elevated recombination and pairing structures during meiotic arrest in yeast of the nuclear division mutant cdc5. Molecular & general genetics : MGG 16 7038388
2023 FOXA1 prolongs S phase and promotes cancer progression in non-small cell lung cancer through upregulation of CDC5L and activation of the ERK1/2 and JAK2 pathways. The Kaohsiung journal of medical sciences 15 37658700
2021 CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. The Journal of biological chemistry 15 34298017
2016 Reduced kinase activity of polo kinase Cdc5 affects chromosome stability and DNA damage response in S. cerevisiae. Cell cycle (Georgetown, Tex.) 14 27565373
2003 hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing. Biochemical and biophysical research communications 14 12927788
1997 Differential expression of elongation factor-2, alpha4 phosphoprotein and Cdc5-like protein in prolactin-dependent/independent rat lymphoid cells. Molecular and cellular endocrinology 14 9296381
2020 Distinct surfaces on Cdc5/PLK Polo-box domain orchestrate combinatorial substrate recognition during cell division. Scientific reports 13 32099015
2004 A cDNA homologue of Schizosaccharomyces pombe cdc5(+) from the mushroom Lentinula edodes: characterization of the cDNA and its expressed product. Biochimica et biophysica acta 12 15488989
2010 Implication of Akt-dependent Prp19 alpha/14-3-3beta/Cdc5L complex formation in neuronal differentiation. Journal of neuroscience research 11 20629186
2003 Distinct domains of human CDC5 direct its nuclear import and association with the spliceosome. Cell biochemistry and biophysics 10 14515018
2014 Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry 9 25263959
2008 Basidiomycete Lentinula edodes CDC5 and a novel interacting protein CIPB bind to a newly isolated target gene in an unusual manner. Fungal genetics and biology : FG & B 9 18448367
2006 Adenoviral delivery of human CDC5 promotes G2/M progression and cell division in neonatal ventricular cardiomyocytes. Gene therapy 9 16482203
2016 Physical Association of Saccharomyces cerevisiae Polo-like Kinase Cdc5 with Chromosomal Cohesin Facilitates DNA Damage Response. The Journal of biological chemistry 8 27325700
2017 Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha. eLife 7 28853395
2011 Phosphorylation of Cdc5 regulates its accumulation. Cell division 7 22204387
2006 Developmental regulator Le.CDC5 of the mushroom Lentinula edodes: analyses of its amount in each of the stages of fruiting-body formation and its distribution in parts of the fruiting bodies. FEMS microbiology letters 7 16842359
2024 m6A modification of CDC5L promotes lung adenocarcinoma progression through transcriptionally regulating WNT7B expression. American journal of cancer research 6 39113868
2024 IGF2BP1 promotes multiple myeloma with chromosome 1q gain via increasing CDC5L expression in an m6A-dependent manner. Genes & diseases 6 39534570
2022 HuR Promotes the Progression of Gastric Cancer through Mediating CDC5L Expression. Disease markers 6 35313568
2010 Cdc5 blocks in vivo Rad53 activity, but not in situ activity (ISA). Cell cycle (Georgetown, Tex.) 6 20962588
2001 Prolactin, interleukin-2 and FGF-2 stimulate expression, nuclear distribution and DNA-binding of rat homolog of pombe Cdc5 in Nb2 T lymphoma cells. Molecular and cellular endocrinology 6 11694351
2020 Identification of CDC5L as bridge gene between chronic obstructive pulmonary disease and lung adenocarcinoma. Epigenomics 5 32543224

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