Affinage

BCAS2

Pre-mRNA-splicing factor SPF27 · UniProt O75934

Length
225 aa
Mass
26.1 kDa
Annotated
2026-06-09
82 papers in source corpus 25 papers cited in narrative 25 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 5/6 claims corpus-supported (83%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BCAS2 is a core component of the PRP19/NTC spliceosome complex that drives constitutive and alternative pre-mRNA splicing across diverse cell types, and it carries out additional splicing-independent regulatory functions in Wnt signaling, DNA repair, and protein stability (PMID:23249746, PMID:39520542). Within the splicing machinery, its C-terminal coiled-coil domain directly binds CDC5L and recruits hPrp19 and PLRG1 to assemble a core splicing sub-complex, a function conserved from Drosophila to human (PMID:23249746). BCAS2 binds RNA directly at 5' splice sites and GA-rich/GAAGAA motifs as mapped by CLIP-seq, and cooperates with the splicing factors hnRNPH1, SRSF3, SRSF7, and hnRNPM to control alternative splicing of specific substrates (PMID:39520542, PMID:40585763, PMID:41680151). Through this activity BCAS2 governs developmental and tissue programs by splicing defined targets: Delta in Drosophila wing Notch signaling (PMID:26091239), Mdm4 to gate p53-dependent survival of zebrafish hematopoietic progenitors (PMID:30482793), Dazl in male meiotic entry (PMID:28128212), Trp53bp1 and Six6os1 during meiotic DSB repair (PMID:39520542), and Syt7/Tcf7l2 in pancreatic β-cell insulin secretion (PMID:37820033). Beyond splicing, BCAS2 directly binds β-catenin via its coiled-coil domain to sequester it in the nucleus and enhance Wnt signaling independently of splicing (PMID:40511787), interacts with NBS1 to promote both NHEJ and HR double-strand break repair (PMID:32963349), acts through the RPA complex in the DNA damage response (PMID:26428007), stabilizes androgen receptor in concert with HSP90 against proteasomal degradation (PMID:25461807), and promotes ubiquitination of HSF4 at K206 (PMID:26319152). BCAS2 was originally identified as a gene amplified in breast cancer (PMID:10403562), and a Bcas2 3'UTR mutation was found in children with hyper-IgM syndrome type 1, where it disrupts antibody class switch recombination (PMID:40585763).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1999 Medium

    Established BCAS2 as a candidate breast cancer gene, framing the question of what this amplified locus encodes.

    Evidence RNA differential display and Southern blot mapping of amplification in breast cancer cell lines

    PMID:10403562

    Open questions at the time
    • No molecular function assigned
    • Amplification correlation not linked to a mechanism
  2. 2002 Medium

    Localized BCAS2 to the nucleus and linked it to a Src-family kinase, providing the first hints of cellular context before its splicing role was known.

    Evidence EGFP fusion localization in breast cancer cells; yeast two-hybrid and cell death assay with BLK in mammary epithelial cells

    PMID:12169396 PMID:12406557

    Open questions at the time
    • BLK interaction not connected to later splicing function
    • Functional significance of nuclear localization unresolved at the time
  3. 2012 High

    Defined BCAS2 as a core splicing complex component, answering how it acts molecularly: its coiled-coil domain binds CDC5L and recruits hPrp19/PLRG1.

    Evidence Drosophila RNAi with human BCAS2 rescue, domain-specific binding assays, and co-immunoprecipitation

    PMID:23249746

    Open questions at the time
    • Direct RNA-binding sites not yet mapped
    • Substrate specificity within the spliceosome undefined
  4. 2014 High

    Extended BCAS2 beyond splicing to protein-stability and transcriptional control, showing it stabilizes androgen receptor via HSP90 and mediates ERRβ-driven cell cycle effects.

    Evidence GST pull-down, reciprocal co-IP, luciferase and ChIP assays in breast cancer cells; parallel fungal ortholog NTC/splicing analysis

    PMID:24391515 PMID:24667650 PMID:25461807

    Open questions at the time
    • Whether AR stabilization is independent of the splicing complex unclear
    • Generality across non-breast tissues untested
  5. 2015 High

    Connected BCAS2 to the DNA damage response and protein degradation pathways, identifying RPA1 binding as required for repair and HSF4-K206 ubiquitination as a degradation route.

    Evidence Maternal depletion in mouse zygotes with RPA1-binding mutant rescue; cycloheximide chase, K206R mutagenesis and ubiquitination assays

    PMID:26091239 PMID:26319152 PMID:26428007

    Open questions at the time
    • Whether BCAS2 is itself a ubiquitin ligase or an adaptor for HSF4 not resolved
    • Mechanistic link between splicing role and RPA-dependent repair unclear
  6. 2016 High

    Identified β-catenin pre-mRNA as a tissue-relevant splicing target, placing BCAS2 upstream of β-catenin in dendrite development.

    Evidence Forebrain conditional knockout, exon array, and β-catenin overexpression rescue in primary neurons

    PMID:27713508

    Open questions at the time
    • Did not distinguish splicing of β-catenin from direct protein-level regulation
    • Splice site recognition mechanism unmapped
  7. 2017 High

    Demonstrated BCAS2 controls cell-type-specific alternative splicing programs, with Dazl isoform regulation gating meiotic entry in male germ cells.

    Evidence Germ-cell conditional knockout with RNA-seq alternative splicing analysis and protein isoform detection; miR-486 targeting in esophageal cancer

    PMID:28128212 PMID:29115564

    Open questions at the time
    • Direct RNA-binding sites still not mapped
    • How target selection is achieved within the NTC undefined
  8. 2018 High

    Linked BCAS2-dependent splicing to a survival pathway, showing Mdm4 mis-splicing triggers p53-mediated apoptosis of hematopoietic progenitors.

    Evidence Zebrafish TALEN knockout, splicing analysis, and p53-suppression rescue; super-resolution localization of the fungal ortholog at microtubules

    PMID:29483520 PMID:30482793

    Open questions at the time
    • Whether the splicing-to-p53 axis operates in mammalian HSPCs untested
    • Cytoplasmic functions seen in fungi not validated for human BCAS2
  9. 2019 Medium

    Showed BCAS2 functions as a nuclear receptor coactivator, binding ERα and enhancing its transcriptional activity downstream of PI3K/AKT.

    Evidence Co-IP, GST pull-down, and AF-1/AF-2 luciferase dissection with signaling inhibitors in breast cancer cells

    PMID:30813351

    Open questions at the time
    • Direct vs complex-mediated coactivation not fully separated
    • Relationship to splicing role unaddressed
  10. 2020 High

    Defined a direct DNA repair function, mapping BCAS2-NBS1 interaction domains and showing enhancement of both NHEJ and HR.

    Evidence GST pull-down, reciprocal co-IP with domain mapping, and functional NHEJ/HR repair assays

    PMID:32963349

    Open questions at the time
    • Whether repair function requires the splicing complex unresolved
    • Order of recruitment relative to MRN complex unclear
  11. 2023 High

    Generalized the PRP19-complex splicing role across reproductive and metabolic tissues, identifying distinct substrate sets in oocytes, granulosa cells, and β-cells.

    Evidence Multiple tissue-specific conditional knockouts with RNA-seq splicing analysis and PRP19-complex co-IP

    PMID:37248466 PMID:37820033 PMID:38085152

    Open questions at the time
    • Basis for tissue-specific target selection not explained
    • Direct RNA contacts in these tissues not yet mapped
  12. 2024 High

    Provided direct RNA-binding evidence, mapping BCAS2 to 5' splice sites and GA-rich motifs and identifying hnRNPH1/SRSF3 partners controlling meiotic DSB-repair splicing targets.

    Evidence CLIP-seq, conditional knockout, RNA-seq, and co-immunoprecipitation in testis

    PMID:39520542

    Open questions at the time
    • How binding-site recognition is regulated unknown
    • Quantitative contribution of each partner not dissected
  13. 2025 High

    Resolved a splicing-independent function and a disease link, showing BCAS2 directly retains β-catenin in the nucleus to enhance Wnt signaling and controls B-cell class switch recombination, with a human hyper-IgM mutation.

    Evidence Zebrafish/MEF knockouts with coiled-coil domain mapping and Wnt reporter assays; AID-Cre conditional knockout with CLIP-seq and interactome proteomics identifying SRSF7

    PMID:40511787 PMID:40585763

    Open questions at the time
    • Switch between splicing and nuclear-retention modes not mechanistically defined
    • Causality of the 3'UTR mutation not established by reconstitution
  14. 2026 Medium

    Identified hnRNPM as a partner directing BCAS2 to pre-mRNA loci, refining how splicing fidelity is achieved in oocytes.

    Evidence hnRNPM genetic ablation, LACE-seq RNA-binding mapping, SCAN-seq, and co-immunoprecipitation

    PMID:41680151

    Open questions at the time
    • Single-lab interaction not reciprocally validated across systems
    • Whether hnRNPM recruits BCAS2 or vice versa unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How BCAS2 partitions between its spliceosomal role and its multiple splicing-independent functions (Wnt nuclear retention, NBS1/RPA-dependent repair, AR/HSF4 protein stability) within a single cell remains unresolved.
  • No structural model integrating coiled-coil binding to CDC5L, β-catenin, and NBS1
  • Regulatory switch between moonlighting functions unknown
  • Whether splicing-independent roles require complex assembly untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 3 GO:0140098 catalytic activity, acting on RNA 3 GO:0060090 molecular adaptor activity 2 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 2
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-162582 Signal Transduction 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-73894 DNA Repair 2
Partners
CDC5LCTNNB1HNRNPH1NBS1PLRG1PRP19RPA1SRSF7
Complex memberships
PRP19/NTC spliceosome complex

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 BCAS2 (DAM1) was identified as a novel gene amplified at chromosome 1p13.3-21 in human breast cancer cell lines MCF-7 and BT-20, encoding a 26-kDa protein upregulated by gene amplification. RNA differential display, Southern blot analysis, chromosomal mapping Cancer letters Medium 10403562
2002 BCAS2 protein localizes to the nucleus, as shown by enhanced green fluorescent protein (EGFP) fusion assay in breast cancer cells. EGFP fusion protein localization assay Cancer letters Medium 12169396
2002 Mouse BCAS2 (mDAM1) interacts with the Src-family kinase BLK in vitro and in vivo, and co-expression of mDAM1 with BLK increases cell death compared to BLK alone in NMuMG mammary epithelial cells, indicating BCAS2 promotes pro-apoptotic activity of BLK. Yeast two-hybrid screen, in vitro protein binding assay, stable transfection with cell death assay Cancer letters Medium 12406557
2012 Drosophila BCAS2 (dBCAS2) is essential for viability and functions in pre-mRNA splicing; its depletion causes impaired splicing and apoptosis. The C-terminal coiled-coil domain of human BCAS2 directly binds CDC5L and recruits hPrp19/PLRG1 to form a core splicing complex. Overexpression of hBCAS2 rescues Drosophila dBCAS2 depletion phenotypes, demonstrating functional conservation. RNAi knockdown in Drosophila, rescue with human BCAS2, direct binding assay (C-terminal coiled-coil domain), co-immunoprecipitation RNA (New York, N.Y.) High 23249746
2014 BCAS2 is a direct transcriptional target of ERRβ and mediates ERRβ-dependent inhibition of FST transcription through downregulation of β-catenin/TCF4 recruitment to the FST promoter. ERRβ-mediated upregulation of BCAS2 downregulates cyclin D1, blocking G1/S transition in breast cancer cells. ChIP cloning, gel supershift assays, co-immunoprecipitation, confocal microscopy, western blotting, quantitative RT-PCR, luciferase assay British journal of cancer Medium 24667650
2014 BCAS2 interacts directly with androgen receptor (AR) and HSP90, stabilizing AR protein from proteasomal degradation via two mechanisms: (1) p53-dependent suppression increasing AR mRNA and protein, and (2) p53-independent inhibition of proteasome degradation through BCAS2-AR-HSP90 complex formation. GST pull-down assay, co-immunoprecipitation, luciferase assay, MTT assay, western blotting, immunohistochemistry British journal of cancer High 25461807
2014 In Ustilago maydis, the SPF27/BCAS2 homologue Num1 functions as a core NTC/PRP19 complex component required for pre-mRNA splicing (global intron retention upon deletion) and additionally interacts with the kinesin motor Kin1 in the cytoplasm, connecting splicing to cytoplasmic vesicle trafficking and polarized growth. RNA-seq (intron retention), yeast two-hybrid/interactome screen, co-immunoprecipitation, genetic phenotypic analysis PLoS genetics Medium 24391515
2015 Maternal BCAS2 maintains genome integrity in mouse early embryos by functioning through the RPA complex: BCAS2 responds to DNA damage in zygotes, and BCAS2 mutants unable to bind RPA1 fail to support DNA repair. Phosphorylated RPA2 cannot localize to DNA damage sites when maternal BCAS2 is disrupted, establishing BCAS2's role in the DNA damage response via RPA. Maternal depletion (morpholino/siRNA injection in zygotes), immunofluorescence, DNA damage assays, mutant rescue experiments, co-immunoprecipitation Development (Cambridge, England) High 26428007
2015 BCAS2 negatively regulates HSF4 protein stability by promoting its ubiquitination at lysine 206; knockdown of BCAS2 increases HSF4 protein half-life and reduces ubiquitination. The HSF4-K206R mutant blocks BCAS2's impact on HSF4 stability, implicating BCAS2 in HSF4 degradation via the ubiquitin-proteasome system. Co-immunoprecipitation, western blotting, cycloheximide chase (protein half-life), site-directed mutagenesis (K206R), ubiquitination assay The international journal of biochemistry & cell biology High 26319152
2015 BCAS2 regulates Delta-Notch signaling in Drosophila wing development through pre-mRNA splicing of Delta; dBCAS2 depletion reduces Delta mRNA, causes accumulation of Delta pre-mRNA, and diminishes transcription of Notch target genes (cut, E(spl)m8). Overexpression of Delta rescues dBCAS2 depletion wing deformation, and ectopic expression of hBCAS2 or dBCAS2 rescues pre-mRNA splicing defects. RNAi knockdown, RT-PCR (pre-mRNA accumulation), genetic rescue experiments, reporter gene assays PloS one High 26091239
2016 Conditional knockout of BCAS2 in mouse forebrain causes dendritic malformation; exon array analysis identified β-catenin pre-mRNA as a splicing target of BCAS2. Overexpression of β-catenin in BCAS2-depleted primary neurons restores dendritic growth, placing BCAS2 upstream of β-catenin in a pathway regulating dendrite development. Conditional knockout mouse model, exon array assay, Golgi staining, BrdU incorporation, DCX immunostaining, β-catenin overexpression rescue Scientific reports High 27713508
2017 BCAS2 is specifically enriched in spermatogonia and is required for alternative splicing in male germ cells and the transition to meiosis. Conditional disruption of Bcas2 in male germ cells alters alternative splicing of 245 genes including Dazl, Ehmt2, and Hmga1; loss of BCAS2 causes decrease in full-length DAZL protein and increase of a short form lacking exon 8, impairing meiosis initiation. Conditional knockout mouse model, alternative splicing analysis (RNA-seq), western blotting, immunofluorescence, histological analysis of spermatogenesis Nature communications High 28128212
2017 miR-486 targets BCAS2 (and CDK4) in esophageal cancer cells; dual-luciferase reporter assay confirmed BCAS2 as a direct target of miR-486. Knockdown of BCAS2 phenocopies miR-486 overexpression, inducing G0/G1 arrest and apoptosis, with upregulation of p21 and caspase-3. Dual-luciferase reporter gene assay, siRNA knockdown, flow cytometry, western blotting Oncology reports Medium 29115564
2018 BCAS2 is essential for hematopoietic stem and progenitor cell (HSPC) maintenance in zebrafish; bcas2 knockout induces abnormal alternative splicing of Mdm4 (producing a pro-apoptotic isoform), leading to p53-mediated HSPC apoptosis. Suppression of p53 rescues the HSPC deficiency, establishing a BCAS2 → Mdm4 splicing → p53 pathway. TALEN-mediated knockout in zebrafish, alternative splicing analysis, p53 suppression rescue experiments, TUNEL assay, in situ hybridization Blood High 30482793
2018 In Ustilago maydis, the SPF27/BCAS2 homologue Num1 localizes predominantly in the nucleus but also in the cytoplasm near microtubules; its cytoplasmic movement depends on interaction with kinesin Kin1 (not Kin3), as shown by super-resolution localization microscopy. Super-resolution localization microscopy (STORM/PALM with tdEosFP fusion), particle tracking analysis, genetic dependency on Kin1 vs Kin3 Scientific reports Medium 29483520
2019 BCAS2 interacts with ERα both in vitro and in vivo and upregulates ERα transcriptional activity through the AF-1 (N-terminal) region of ERα directly, and indirectly through the AF-2 (C-terminal) region by acting in concert with AF-2 coactivators. BCAS2 is regulated preferentially by the PI3K/AKT signaling pathway in breast cancer cells. Co-immunoprecipitation, GST pull-down (protein-protein interaction), luciferase reporter assay, signal transduction inhibitor experiments International journal of molecular sciences Medium 30813351
2020 BCAS2 enhances both non-homologous end joining (NHEJ) and homologous recombination (HR) DNA double-strand break repair pathways by directly interacting with NBS1; this interaction involves the BCAS2 N-terminus and both the NBS1 N- and C-termini, as defined by GST pull-down and co-immunoprecipitation assays. GST pull-down assay, co-immunoprecipitation, precise end-joining assay (NHEJ), flow cytometry (HR), immunofluorescence, radiation-induced DSB repair assay British journal of cancer High 32963349
2022 BCAS2 (as an RNA binding protein) regulates the expression of circular RNA circ_002363 by interacting with Pre-DNA2, the host gene of circ_002363, in bronchial epithelial cells exposed to neodymium oxide nanoparticles, as shown by RNA pull-down and western blot assays. RNA pull-down assay, western blot, RT-qPCR, functional DNA damage assays The Science of the total environment Low 36526188
2023 BCAS2 regulates oocyte meiotic prophase I by participating in alternative splicing through the PRP19 complex (with CDC5L and PRP19); conditional knockout of Bcas2 in oocytes during pachytene phase causes infertility, primordial follicle depletion, and abnormal alternative splicing of Dazl and Diaph2. Conditional knockout mouse model (Stra8-GFPCre), RNA-seq alternative splicing analysis, immunostaining, fertility testing FASEB journal High 38085152
2023 BCAS2 regulates granulosa cell survival by participating in alternative splicing of E2f3 and Flt3l mRNA through the PRP19 complex (with CDC5L and PRP19); Bcas2 knockout in granulosa cells causes cell cycle arrest, DNA damage, apoptosis, and abnormal RPA1 staining. Conditional knockout mouse model, RNA-seq, BrdU incorporation assay, immunostaining, co-immunoprecipitation (PRP19 complex) Journal of ovarian research High 37248466
2023 BCAS2 participates in insulin synthesis and secretion in pancreatic β-cells via alternative splicing; Bcas2 conditional knockout in β-cells causes glucose intolerance and decreased insulin secretion granules, linked to abnormal splicing of Syt7 and Tcf7l2 pre-mRNA. Conditional knockout mouse model (Bcas2 f/f-βKO), glucose tolerance test, insulin secretion assay, immunohistochemistry, electron microscopy of secretion granules, alternative splicing analysis Endocrinology High 37820033
2024 BCAS2 directly binds 5' splice sites (5'SS) of introns and GA-rich regions in testis (mapped by CLIP-seq); it regulates alternative splicing of Trp53bp1 (53BP1) and Six6os1 during meiotic prophase I DSB repair and synapsis. BCAS2 interacts with hnRNPH1 and SRSF3 to orchestrate Trp53bp1 expression via alternative splicing. CLIP-seq (crosslinking immunoprecipitation and sequencing), conditional knockout mouse model, co-immunoprecipitation, RNA-seq Cellular and molecular life sciences High 39520542
2025 BCAS2 promotes primitive hematopoiesis by directly binding to β-catenin via its coiled-coil domains and sequestering β-catenin within the nucleus, thereby enhancing Wnt/β-catenin signaling. BCAS2 deficiency reduces β-catenin nuclear accumulation without affecting β-catenin pre-mRNA splicing, revealing a splicing-independent nuclear retention function. bcas2 knockout in zebrafish and mouse embryonic fibroblasts, co-immunoprecipitation, domain mapping (coiled-coil), β-catenin localization assay, Wnt reporter assay, splicing analysis eLife High 40511787
2025 BCAS2 regulates antibody class switch recombination (CSR) in activated B cells by interacting with SRSF7 at a conserved circular domain to form a complex that controls alternative splicing of genes involved in post-switch transcription. BCAS2 binds RNA at GAAGAA motifs (identified by CLIP-seq). A mutation at the 3'UTR of Bcas2 was found in children with hyper-IgM syndrome type 1, with similar AS and CSR patterns. Conditional knockout mouse model (AID-Cre Bcas2 fl/fl), CLIP-seq, RNA-sequencing, co-immunoprecipitation, interactome proteomics Exploration (Beijing, China) High 40585763
2026 BCAS2 interacts with hnRNPM and modulates its binding to pre-mRNA loci to control alternative splicing during oocyte development; LACE-seq revealed hnRNPM-binding sites at single-nucleotide resolution and showed that hnRNPM-BCAS2 interaction is required for splicing fidelity in growing oocytes. Genetic ablation of hnRNPM, LACE-seq (RNA-binding site mapping), SCAN-seq (single-cell), co-immunoprecipitation Nature communications Medium 41680151

Source papers

Stage 0 corpus · 82 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Molecular cell 258 15664196
2006 The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 230 16415853
2010 Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B. The Journal of cell biology 174 20479468
2006 The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Proceedings of the National Academy of Sciences of the United States of America 157 16777964
2010 The Dam1 complex confers microtubule plus end-tracking activity to the Ndc80 kinetochore complex. The Journal of cell biology 148 20479465
2002 Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. The EMBO journal 148 11782438
2001 Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. The Journal of cell biology 144 11724818
2007 Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nature cell biology 131 17572669
2008 Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation. Nature cell biology 127 18364702
2006 Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Current biology : CB 85 16890524
2007 Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nature structural & molecular biology 81 17643123
2008 Different assemblies of the DAM1 complex follow shortening microtubules by distinct mechanisms. Proceedings of the National Academy of Sciences of the United States of America 79 18460602
2008 The Dam1 ring binds microtubules strongly enough to be a processive as well as energy-efficient coupler for chromosome motion. Proceedings of the National Academy of Sciences of the United States of America 75 18824692
2012 Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes. The Journal of cell biology 69 23277429
2013 Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules. Proceedings of the National Academy of Sciences of the United States of America 62 23610433
2017 BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nature communications 58 28128212
2011 Chromatin signaling to kinetochores: transregulation of Dam1 methylation by histone H2B ubiquitination. Cell 56 21884933
2009 Ipl1-dependent phosphorylation of Dam1 is reduced by tension applied on kinetochores. Journal of cell science 56 19923271
2022 BACE-1 inhibition facilitates the transition from homeostatic microglia to DAM-1. Science advances 51 35714196
2015 Maternal BCAS2 protects genomic integrity in mouse early embryonic development. Development (Cambridge, England) 48 26428007
2014 Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation. Nature communications 47 25236177
2009 A Dam1-based artificial kinetochore is sufficient to promote chromosome segregation in budding yeast. Nature cell biology 46 19684577
2017 The Ndc80 complex bridges two Dam1 complex rings. eLife 44 28191870
2007 The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast. Journal of cell science 42 17881496
2017 miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2. Oncology reports 41 29115564
2015 The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling. Nature communications 41 26560693
2014 ERRβ signalling through FST and BCAS2 inhibits cellular proliferation in breast cancer cells. British journal of cancer 41 24667650
2011 The requirement for the Dam1 complex is dependent upon the number of kinetochore proteins and microtubules. Current biology : CB 40 21549601
2008 Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2. Molecular biology of the cell 40 18256284
2017 Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1. Genome biology and evolution 39 28472331
2018 BCAS2 is essential for hematopoietic stem and progenitor cell maintenance during zebrafish embryogenesis. Blood 32 30482793
2011 The essentiality of the fungus-specific Dam1 complex is correlated with a one-kinetochore-one-microtubule interaction present throughout the cell cycle, independent of the nature of a centromere. Eukaryotic cell 28 21571923
1999 Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. Cancer letters 27 10403562
2023 Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. Science (New York, N.Y.) 26 38060647
2009 Structure-function insights into the yeast Dam1 kinetochore complex. Journal of cell science 24 19889968
2016 Molecular architecture of the Dam1 complex-microtubule interaction. Open biology 23 26962051
2012 BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. RNA (New York, N.Y.) 23 23249746
2010 A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast. Proceedings of the National Academy of Sciences of the United States of America 20 20624975
2002 Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer. Cancer letters 20 12169396
2020 Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments. Current biology : CB 19 32946748
2014 BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. British journal of cancer 17 25461807
2017 Mps1 promotes chromosome meiotic chromosome biorientation through Dam1. Molecular biology of the cell 15 29237818
2014 The SPF27 homologue Num1 connects splicing and kinesin 1-dependent cytoplasmic trafficking in Ustilago maydis. PLoS genetics 15 24391515
2019 BCAS2 Enhances Carcinogenic Effects of Estrogen Receptor Alpha in Breast Cancer Cells. International journal of molecular sciences 14 30813351
2023 DASH/Dam1 complex mutants stabilize ploidy in histone-humanized yeast by weakening kinetochore-microtubule attachments. The EMBO journal 13 36651597
2017 The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast. Scientific reports 13 28928489
2022 Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load. The Journal of cell biology 12 35353161
2021 Circular RNA NEK6 contributes to the development of non-small-cell lung cancer by competitively binding with miR-382-5p to elevate BCAS2 expression at post-transcriptional level. BMC pulmonary medicine 12 34663267
2018 Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis. Scientific reports 12 29483520
2008 Fission yeast dam1-A8 mutant is resistant to and rescued by an anti-microtubule agent. Biochemical and biophysical research communications 12 18262494
2008 Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction. Molecular biology of the cell 12 18632983
2015 BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. PloS one 11 26091239
2022 Circ_002363 is regulated by the RNA binding protein BCAS2 and inhibits neodymium oxide nanoparticle-induced DNA damage by non-homologous end-joining repair. The Science of the total environment 10 36526188
2018 Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit in Saccharomycescerevisiae. Genetics 10 30546002
2020 BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair. British journal of cancer 9 32963349
2018 Identification of potential inhibitors against nuclear Dam1 complex subunit Ask1 of Candida albicans using virtual screening and MD simulations. Computational biology and chemistry 8 29346071
2016 Conditional Knockout of Breast Carcinoma Amplified Sequence 2 (BCAS2) in Mouse Forebrain Causes Dendritic Malformation via β-catenin. Scientific reports 8 27713508
2015 BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. The international journal of biochemistry & cell biology 8 26319152
2010 A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression. Biotechnology progress 8 20730753
2002 Dam1 is the right one: phosphoregulation of kinetochore biorientation. Developmental cell 7 12431367
2024 BCAS2 regulates oocyte meiotic prophase I by participating in mRNA alternative splicing. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 6 38085152
2021 Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I. Open biology 6 33529549
2020 Knockdown of long non-coding RNA plasmacytoma variant translocation 1 inhibits cell proliferation while promotes cell apoptosis via regulating miR-486-mediated CDK4 and BCAS2 in multiple myeloma. Irish journal of medical science 6 31900844
2012 Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast. Journal of cell science 6 22375062
2023 BCAS2 Participates in Insulin Synthesis and Secretion via mRNA Alternative Splicing in Mice. Endocrinology 5 37820033
2023 Molecular characterization, expression patterns and cellular localization of BCAS2 gene in male Hezuo pig. PeerJ 5 37901468
2019 Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast. Chromosoma 5 30903360
2025 Spindle integrity is regulated by a phospho-dependent interaction between the Ndc80 and Dam1 kinetochore complexes. PLoS genetics 4 40184422
2025 The RNA Binding Protein Bcas2 is Required for Antibody Class Switch in Activated-B Cells. Exploration (Beijing, China) 4 40585763
2023 BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing. Journal of ovarian research 4 37248466
2002 Mouse DAM1 regulates pro-apoptotic activity of BLK in mammary epithelial cells. Cancer letters 4 12406557
2016 The Nuclear Transport Factor Kap121 Is Required for Stability of the Dam1 Complex and Mitotic Kinetochore Bi-orientation. Cell reports 3 26947076
2024 BCAS2 and hnRNPH1 orchestrate alternative splicing for DNA double-strand break repair and synapsis in meiotic prophase I. Cellular and molecular life sciences : CMLS 2 39520542
2023 Increased Sampling and Intracomplex Homologies Favor Vertical Over Horizontal Inheritance of the Dam1 Complex. Genome biology and evolution 2 36790109
2010 The Dad1 subunit of the yeast kinetochore Dam1 complex is an intrinsically disordered protein. Biochemical and biophysical research communications 2 20727855
2008 Production and initial characterization of Dad1p, a component of the Dam1-DASH kinetochore complex. PloS one 2 19065263
2025 BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus. eLife 1 40511787
2024 Chromosome segregation: Mps1 and Dam1 battle to bind a shared interaction site at the kinetochore. Current biology : CB 1 38834024
2026 hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development. Nature communications 0 41680151
2025 BcAS2 Regulates Leaf Adaxial Polarity Development in Non-Heading Chinese Cabbage by Directly Activating BcPHB Transcription. Plants (Basel, Switzerland) 0 40284095
2025 Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation. PLoS genetics 0 40523001
2020 Structural view of the yeast Dam1 complex, a ring-shaped molecular coupler for the dynamic microtubule end. Essays in biochemistry 0 32579171

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