Affinage

BCAS2

Pre-mRNA-splicing factor SPF27 · UniProt O75934

Length
225 aa
Mass
26.1 kDa
Annotated
2026-04-28
83 papers in source corpus 21 papers cited in narrative 21 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BCAS2 is a nuclear protein that functions as a core subunit of the PRP19/CDC5L spliceosome complex and regulates alternative pre-mRNA splicing of diverse developmental and tissue-specific genes, while also participating in DNA damage repair and Wnt/β-catenin signaling. Within the PRP19 complex, BCAS2 binds CDC5L through its C-terminal coiled-coil domain and directly contacts 5' splice sites of introns with a preference for GA-rich (GAAGAA) motifs, cooperating with co-splicing factors hnRNPH1, SRSF3, and SRSF7 to orchestrate context-specific splicing of substrates including Dazl, β-catenin, Mdm4, Trp53bp1, and Syt7 — thereby governing meiosis, hematopoiesis, neuronal dendrite growth, pancreatic β-cell insulin secretion, and antibody class-switch recombination (PMID:23249746, PMID:28128212, PMID:27713508, PMID:30482793, PMID:39520542, PMID:40585763, PMID:37820033). Independent of its splicing role, BCAS2 directly binds β-catenin via coiled-coil domains to sequester it in the nucleus and promote Wnt signaling during hematopoietic differentiation, and it facilitates DNA double-strand break repair through interactions with both the RPA complex and NBS1, enhancing NHEJ and homologous recombination pathways (PMID:40511787, PMID:26428007, PMID:32963349). BCAS2 also stabilizes androgen receptor protein through an HSP90-containing complex and negatively regulates HSF4 stability via ubiquitination (PMID:25461807, PMID:26319152).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1999 Low

    The initial discovery established BCAS2 as a novel nuclear protein amplified in breast cancer, but its molecular function was unknown.

    Evidence RNA differential display with EGFP fusion localization in breast cancer cell lines

    PMID:10403562

    Open questions at the time
    • No functional activity identified
    • Localization based on single overexpression method
    • No endogenous protein detection
  2. 2002 Low

    Early interaction studies identified BLK kinase as a binding partner and linked BCAS2 overexpression to cell death, but the physiological relevance remained unclear.

    Evidence Yeast two-hybrid screening and stable transfection in NMuMG cells

    PMID:12406557

    Open questions at the time
    • BLK interaction not validated by reciprocal methods
    • Overexpression-only functional data
    • No endogenous context tested
  3. 2012 High

    A pivotal advance established BCAS2 as a core spliceosome component by showing its C-terminal coiled-coil domain directly binds CDC5L to recruit PRP19/PLRG1, with cross-species rescue confirming functional conservation.

    Evidence Drosophila RNAi knockdown rescued by human BCAS2; co-IP and domain-deletion mapping

    PMID:23249746

    Open questions at the time
    • Specific RNA substrates of the complex not identified
    • Structural basis of the coiled-coil interaction unresolved
    • Whether BCAS2 contacts RNA directly was unknown
  4. 2014 Medium

    Two non-splicing functions were uncovered: BCAS2 stabilizes androgen receptor through an HSP90-containing complex and destabilizes HSF4 through ubiquitination at K206, revealing BCAS2 as a regulator of protein turnover.

    Evidence Co-IP, GST pull-down, cycloheximide chase, ubiquitination assays, and site-directed mutagenesis

    PMID:25461807 PMID:26319152

    Open questions at the time
    • Whether BCAS2 functions as a direct E3 ligase or recruits one for HSF4 ubiquitination is unresolved
    • AR stabilization mechanism relies on single-lab data
    • In vivo relevance of AR-HSP90-BCAS2 complex not tested
  5. 2015 High

    BCAS2 was shown to function in DNA damage repair independently of splicing, interacting with RPA1 to recruit phosphorylated RPA2 to damage sites in early embryos, and was found to facilitate Delta pre-mRNA splicing in Drosophila wing development.

    Evidence Maternal siRNA depletion with binding-deficient mutants in mouse embryos; Drosophila RNAi with Delta rescue

    PMID:26091239 PMID:26428007

    Open questions at the time
    • Whether the DNA repair and splicing functions are mechanistically separable was unclear
    • RPA interaction domain on BCAS2 not mapped at high resolution
  6. 2016 High

    Conditional knockout in mouse forebrain demonstrated that BCAS2 regulates β-catenin splicing to control neuronal dendritic growth, establishing the first mammalian tissue-specific splicing substrate.

    Evidence Conditional KO mice with exon array, Golgi staining, and β-catenin overexpression rescue

    PMID:27713508

    Open questions at the time
    • Which specific β-catenin exon/intron is mis-spliced was not defined
    • Whether BCAS2 acts directly on β-catenin pre-mRNA or through co-factors was unknown
  7. 2017 High

    BCAS2's role as a master splicing regulator of meiosis was established when conditional knockout in male germ cells blocked the transition to meiosis prophase I by mis-splicing Dazl, Ehmt2, and Hmga1.

    Evidence Conditional KO mouse with RNA-seq alternative splicing analysis and western blot for DAZL isoforms

    PMID:28128212

    Open questions at the time
    • Whether BCAS2 directly binds these pre-mRNAs or acts indirectly was unresolved
    • How BCAS2 selects specific exons (e.g. Dazl exon 8) was unknown
  8. 2018 High

    In zebrafish, BCAS2 was shown to maintain hematopoietic stem cells by preventing aberrant Mdm4 splicing that otherwise triggers p53-dependent apoptosis, revealing a conserved splicing-p53 axis.

    Evidence TALEN knockout zebrafish with p53 suppression rescue and Mdm4 splicing RT-PCR

    PMID:30482793

    Open questions at the time
    • Whether BCAS2 directly splices Mdm4 or acts through PRP19 complex partners was not distinguished
    • Relevance to mammalian hematopoiesis not yet tested at this point
  9. 2020 Medium

    BCAS2's DNA repair function was extended beyond RPA to include direct interaction with NBS1, promoting both NHEJ and HR pathways, broadening its role as a dual splicing-repair factor.

    Evidence GST pull-down, co-IP, precise end-joining and HR flow cytometry assays in prostate cancer cells

    PMID:32963349

    Open questions at the time
    • Whether BCAS2-NBS1 and BCAS2-RPA interactions occur in the same complex is unknown
    • In vivo DNA repair phenotype not tested with conditional KO
  10. 2023 Medium

    BCAS2's tissue-specific splicing repertoire was expanded to pancreatic β-cells (Syt7, Tcf7l2 substrates affecting insulin secretion) and granulosa cells (E2f3, Flt3l substrates affecting cell survival), establishing BCAS2 as a broadly required tissue-specific splicing regulator.

    Evidence Conditional KO mice with glucose tolerance tests and insulin assays; granulosa KO with RNA-seq and cell line validation

    PMID:37248466 PMID:37820033

    Open questions at the time
    • Direct RNA binding at these substrates not confirmed by CLIP
    • Whether PRP19 complex composition varies between tissues is unknown
  11. 2024 High

    CLIP-seq defined BCAS2's RNA-binding specificity at nucleotide resolution, revealing direct binding to 5' splice sites with GA-rich motif preference and identifying co-splicing partners hnRNPH1 and SRSF3 that cooperate to regulate 53BP1 and Six6os1 splicing during meiosis.

    Evidence CLIP-seq in mouse spermatocytes combined with conditional KO, co-IP, and RNA-seq

    PMID:38085152 PMID:39520542

    Open questions at the time
    • Structural basis of BCAS2-RNA recognition unknown
    • How BCAS2 coordinates with hnRNPH1 versus SRSF3 at different substrates is unresolved
  12. 2025 High

    A splicing-independent function was rigorously separated: BCAS2 directly binds β-catenin through coiled-coil domains to sequester it in the nucleus and activate Wnt signaling, essential for hematopoietic differentiation in both zebrafish and mouse.

    Evidence Conditional KO in two model organisms with domain-deletion mutants, nuclear fractionation, and Wnt reporter assays

    PMID:40511787

    Open questions at the time
    • Whether the β-catenin sequestration and PRP19 complex membership are mutually exclusive is unresolved
    • Structural details of the BCAS2-β-catenin interface are lacking
  13. 2025 High

    BCAS2 was shown to be required for antibody class-switch recombination in B cells, interacting with SRSF7 via a conserved circular domain and binding the GAAGAA RNA motif, extending its immune function beyond hematopoietic maintenance.

    Evidence Conditional KO mice (AID-Cre), CLIP-seq, interactome proteomics, B cell activation assays

    PMID:40585763

    Open questions at the time
    • Specific post-switch transcript splicing events regulated by BCAS2-SRSF7 not fully catalogued
    • Whether BCAS2 affects somatic hypermutation in addition to CSR is untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major unresolved questions include: (1) the structural basis for BCAS2's dual RNA-binding and protein-sequestration functions, (2) how tissue-specific co-factor selection (hnRNPH1/SRSF3/SRSF7) is determined, and (3) whether the splicing and DNA repair functions are mechanistically coupled or independent in vivo.
  • No high-resolution structure of BCAS2 alone or in complex
  • Mechanism of tissue-specific co-factor selection unknown
  • Splicing-repair coupling not tested in single experimental system

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140098 catalytic activity, acting on RNA 9 GO:0003723 RNA binding 3 GO:0098772 molecular function regulator activity 3 GO:0140110 transcription regulator activity 1
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-8953854 Metabolism of RNA 9 R-HSA-73894 DNA Repair 3 R-HSA-1266738 Developmental Biology 2 R-HSA-1474165 Reproduction 2 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2
Partners
CDC5LHNRNPH1NBS1PLRG1PRP19RPA1SRSF3SRSF7
Complex memberships
BCAS2-AR-HSP90 complexPRP19/CDC5L (NTC) spliceosome complex

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2017 BCAS2 regulates alternative mRNA splicing in spermatogonia, and conditional disruption of Bcas2 in male germ cells impairs spermatogenesis and blocks the transition to meiosis prophase I. At least three spermatogenesis-related genes (Dazl, Ehmt2, Hmga1) show altered alternative splicing, including loss of full-length DAZL protein with accumulation of a short isoform lacking exon 8. Conditional knockout mouse model, RNA-seq alternative splicing analysis, western blot, immunofluorescence Nature communications High 28128212
2015 Maternal BCAS2 is required for genome integrity in mouse early embryos; it functions through the RPA complex for DNA damage response. BCAS2 mutants unable to bind RPA1 fail to repair DNA during the zygotic stage, and phosphorylated RPA2 cannot localize to DNA damage sites when maternal BCAS2 is disrupted. Maternal depletion (siRNA microinjection), immunofluorescence, co-immunoprecipitation, GST pull-down with mutant BCAS2 Development (Cambridge, England) High 26428007
2012 Drosophila BCAS2 (dBCAS2) is essential for pre-mRNA splicing and viability. The C-terminal coiled-coil domain of human BCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core splicing complex. Overexpression of hBCAS2 rescues dBCAS2 knockdown phenotypes (larval lethality, wing deformities, impaired splicing). RNAi knockdown in Drosophila, rescue with human BCAS2, co-immunoprecipitation, domain-deletion mapping, splicing assays RNA (New York, N.Y.) High 23249746
2018 BCAS2 is essential for hematopoietic stem and progenitor cell (HSPC) maintenance in zebrafish. BCAS2 knockout induces abnormal alternative splicing of Mdm4, predisposing cells to p53-mediated apoptosis; HSPC defects are rescued by p53 suppression. TALEN-mediated knockout zebrafish, in situ hybridization, flow cytometry, p53 suppression rescue experiment, RT-PCR for Mdm4 splicing Blood High 30482793
2016 Conditional knockout of BCAS2 in mouse forebrain causes dendritic malformation via impaired β-catenin splicing regulation. Exon array assay identified β-catenin as a target of BCAS2 splicing regulation; overexpression of β-catenin in BCAS2-depleted primary neurons restores dendritic growth. Conditional knockout mice, exon array, Golgi staining, overexpression rescue, Morris water maze, BrdU incorporation Scientific reports High 27713508
2015 BCAS2 regulates Delta-Notch signaling activity in Drosophila wing development by facilitating Delta pre-mRNA splicing. Depletion of dBCAS2 reduces Delta mRNA, causes accumulation of Delta pre-mRNA, and diminishes transcription of Notch target genes (cut, E(spl)m8); ectopic Delta expression rescues wing deformation caused by dBCAS2 depletion. RNAi knockdown in Drosophila, RT-PCR for pre-mRNA splicing, rescue experiments with hBCAS2/dBCAS2/Delta overexpression PloS one High 26091239
2014 BCAS2 interacts with androgen receptor (AR) and HSP90 to stabilize AR protein from proteasome degradation. BCAS2 upregulates AR through two mechanisms: (1) p53-dependent suppression increasing AR mRNA/protein, and (2) p53-independent inhibition of proteasome degradation via a BCAS2-AR-HSP90 complex. GST pull-down, co-immunoprecipitation, luciferase reporter assay, MTT assay, immunohistochemistry British journal of cancer Medium 25461807
2014 ERRβ-mediated upregulation of BCAS2 downregulates β-catenin/TCF4 recruitment to the FST promoter, inhibiting FST transcription and blocking G1-S transition via cyclin D1 downregulation in breast cancer cells. ChIP cloning, gel supershift, co-immunoprecipitation, western blot, qRT-PCR, confocal microscopy British journal of cancer Medium 24667650
2019 BCAS2 interacts with ERα through its N-terminal region (AF-1) and upregulates ERα transcriptional activity both directly (via AF-1) and indirectly (via AF-2 coactivators). BCAS2 is preferentially regulated by PI3K/AKT signaling in breast cancer cells. Protein-protein interaction assay, luciferase reporter assay, signal transduction pathway inhibitors, proliferation/migration assays International journal of molecular sciences Medium 30813351
2020 BCAS2 promotes DNA double-strand break (DSB) repair by interacting with NBS1 via both the BCAS2 N-terminus and NBS1 N- and C-termini, enhancing both NHEJ and HR repair pathways in prostate cancer cells. GST pull-down, co-immunoprecipitation, precise end-joining assay, flow cytometry for HR, immunofluorescence, western blot British journal of cancer Medium 32963349
2015 BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. Lysine 206 of HSF4 is the key ubiquitination residue; the HSF4-K206R mutant blocks BCAS2's effect on HSF4 stability. BCAS2 knockdown increases HSF4 protein half-life by reducing its ubiquitination. Co-immunoprecipitation, ubiquitination assay, site-directed mutagenesis, cycloheximide chase, knockdown The international journal of biochemistry & cell biology Medium 26319152
2002 Mouse BCAS2 (mDAM1) was identified as a BLK-interacting protein by yeast two-hybrid screening; in vitro protein binding confirmed the interaction. Stable expression of mDAM1 in NMuMG mammary epithelial cells promoted cell death under serum starvation, and co-expression of mDAM1 with BLK increased cell death compared to BLK alone. Yeast two-hybrid, in vitro protein binding, stable transfection, cell death assay Cancer letters Low 12406557
1999 BCAS2 (DAM1) was identified as a novel nuclear protein amplified at chromosome 1p13.3-21 in breast cancer cell lines; EGFP fusion assay revealed the protein localizes to the nucleus. RNA differential display, Southern blot, EGFP fusion localization assay Cancer letters Low 10403562
2002 BCAS2 protein localizes to the nucleus, as demonstrated by enhanced green fluorescent protein (EGFP) fusion assay in primary breast cancer tissue study. EGFP fusion protein assay Cancer letters Low 12169396
2022 BCAS2 (RNA-binding protein) regulates the formation of circular RNA circ_002363 by interacting with Pre-DNA2 (the host gene pre-mRNA) in cells exposed to neodymium oxide nanoparticles, affecting DNA damage repair via the NHEJ pathway. RNA pull-down, western blot, functional DNA damage assays The Science of the total environment Low 36526188
2023 BCAS2 participates in insulin synthesis and secretion in pancreatic β cells through mRNA alternative splicing of Syt7 and Tcf7l2 pre-mRNA; conditional β-cell knockout mice develop glucose intolerance with decreased insulin secretion granules. Conditional knockout mice, glucose tolerance test, insulin secretion assays, RT-PCR for splicing Endocrinology Medium 37820033
2024 BCAS2 regulates oocyte meiotic prophase I via alternative splicing; conditional knockout of Bcas2 in oocytes during the pachytene phase causes infertility, DNA damage, apoptosis of primordial follicles, and failure to progress to diplotene/dictyotene stages. BCAS2 mediates AS of Dazl and Diaph2 in ovaries together with CDC5L and PRP19. Conditional knockout mice (Stra8-GFPCre), RNA-seq, immunofluorescence, TUNEL assay, fertility tests FASEB journal High 38085152
2023 BCAS2 regulates granulosa cell survival through alternative splicing of E2f3 and Flt3l mRNA via the PRP19 complex (with CDC5L and PRP19). Bcas2 conditional knockout in granulosa cells causes cell cycle arrest, DNA damage, and increased apoptosis, with abnormal RPA1 staining. Conditional knockout mice, RNA-seq, BrdU incorporation, TUNEL, immunofluorescence, KGN cell line validation Journal of ovarian research Medium 37248466
2024 BCAS2 directly binds 5' splice sites (5'SS) of introns with a preference for GA-rich regions (mapped by CLIP-seq). BCAS2 regulates alternative splicing of Trp53bp1 (53BP1) and Six6os1, affecting DNA double-strand break repair and synapsis during meiotic prophase I. BCAS2 interacts with hnRNPH1 and SRSF3 to orchestrate Trp53bp1 expression via AS. Conditional knockout mice, CLIP-seq, co-immunoprecipitation, RNA-seq, immunofluorescence Cellular and molecular life sciences High 39520542
2025 BCAS2 is essential for primitive hematopoiesis by sequestering β-catenin within the nucleus, independent of its role in β-catenin pre-mRNA splicing. BCAS2 directly binds β-catenin via its coiled-coil domains, promoting Wnt/β-catenin nuclear retention and signaling required for hematopoietic progenitor differentiation. Conditional knockout in zebrafish and mouse embryos, co-immunoprecipitation, domain-deletion mutants, β-catenin nuclear fractionation, Wnt reporter assay eLife High 40511787
2025 BCAS2 is required for antibody class switch recombination (CSR) in activated B cells. BCAS2 interacts with SRSF7 via a conserved circular domain to regulate alternative splicing of genes involved in post-switch transcription. The GAAGAA motif was identified as the BCAS2 RNA-binding motif by CLIP-seq. Conditional knockout mice (AID-Cre Bcas2 fl/fl), CLIP-seq, RNA-seq, interactome proteomics, B cell activation assays Exploration (Beijing, China) High 40585763

Source papers

Stage 0 corpus · 83 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Molecular cell 257 15664196
2006 The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 230 16415853
2010 Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B. The Journal of cell biology 173 20479468
2006 The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Proceedings of the National Academy of Sciences of the United States of America 157 16777964
2002 Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. The EMBO journal 148 11782438
2010 The Dam1 complex confers microtubule plus end-tracking activity to the Ndc80 kinetochore complex. The Journal of cell biology 146 20479465
2001 Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. The Journal of cell biology 144 11724818
2007 Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nature cell biology 131 17572669
2008 Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation. Nature cell biology 126 18364702
2006 Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Current biology : CB 84 16890524
2007 Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nature structural & molecular biology 81 17643123
2008 Different assemblies of the DAM1 complex follow shortening microtubules by distinct mechanisms. Proceedings of the National Academy of Sciences of the United States of America 79 18460602
2008 The Dam1 ring binds microtubules strongly enough to be a processive as well as energy-efficient coupler for chromosome motion. Proceedings of the National Academy of Sciences of the United States of America 75 18824692
2012 Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes. The Journal of cell biology 67 23277429
2013 Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules. Proceedings of the National Academy of Sciences of the United States of America 60 23610433
2017 BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nature communications 57 28128212
2011 Chromatin signaling to kinetochores: transregulation of Dam1 methylation by histone H2B ubiquitination. Cell 56 21884933
2009 Ipl1-dependent phosphorylation of Dam1 is reduced by tension applied on kinetochores. Journal of cell science 56 19923271
2022 BACE-1 inhibition facilitates the transition from homeostatic microglia to DAM-1. Science advances 50 35714196
2015 Maternal BCAS2 protects genomic integrity in mouse early embryonic development. Development (Cambridge, England) 48 26428007
2014 Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation. Nature communications 47 25236177
2009 A Dam1-based artificial kinetochore is sufficient to promote chromosome segregation in budding yeast. Nature cell biology 45 19684577
2017 The Ndc80 complex bridges two Dam1 complex rings. eLife 44 28191870
2007 The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast. Journal of cell science 42 17881496
2017 miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2. Oncology reports 41 29115564
2015 The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling. Nature communications 41 26560693
2014 ERRβ signalling through FST and BCAS2 inhibits cellular proliferation in breast cancer cells. British journal of cancer 41 24667650
2011 The requirement for the Dam1 complex is dependent upon the number of kinetochore proteins and microtubules. Current biology : CB 40 21549601
2008 Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2. Molecular biology of the cell 40 18256284
2017 Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1. Genome biology and evolution 37 28472331
2018 BCAS2 is essential for hematopoietic stem and progenitor cell maintenance during zebrafish embryogenesis. Blood 32 30482793
2011 The essentiality of the fungus-specific Dam1 complex is correlated with a one-kinetochore-one-microtubule interaction present throughout the cell cycle, independent of the nature of a centromere. Eukaryotic cell 27 21571923
1999 Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. Cancer letters 27 10403562
2009 Structure-function insights into the yeast Dam1 kinetochore complex. Journal of cell science 24 19889968
2016 Molecular architecture of the Dam1 complex-microtubule interaction. Open biology 23 26962051
2012 BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. RNA (New York, N.Y.) 23 23249746
2023 Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. Science (New York, N.Y.) 22 38060647
2010 A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast. Proceedings of the National Academy of Sciences of the United States of America 20 20624975
2002 Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer. Cancer letters 20 12169396
2020 Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments. Current biology : CB 18 32946748
2014 BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. British journal of cancer 17 25461807
2014 The SPF27 homologue Num1 connects splicing and kinesin 1-dependent cytoplasmic trafficking in Ustilago maydis. PLoS genetics 15 24391515
2019 BCAS2 Enhances Carcinogenic Effects of Estrogen Receptor Alpha in Breast Cancer Cells. International journal of molecular sciences 14 30813351
2017 Mps1 promotes chromosome meiotic chromosome biorientation through Dam1. Molecular biology of the cell 13 29237818
2023 DASH/Dam1 complex mutants stabilize ploidy in histone-humanized yeast by weakening kinetochore-microtubule attachments. The EMBO journal 12 36651597
2021 Circular RNA NEK6 contributes to the development of non-small-cell lung cancer by competitively binding with miR-382-5p to elevate BCAS2 expression at post-transcriptional level. BMC pulmonary medicine 12 34663267
2018 Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis. Scientific reports 12 29483520
2017 The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast. Scientific reports 12 28928489
2008 Fission yeast dam1-A8 mutant is resistant to and rescued by an anti-microtubule agent. Biochemical and biophysical research communications 12 18262494
2008 Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction. Molecular biology of the cell 12 18632983
2022 Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load. The Journal of cell biology 11 35353161
2015 BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. PloS one 11 26091239
2022 Circ_002363 is regulated by the RNA binding protein BCAS2 and inhibits neodymium oxide nanoparticle-induced DNA damage by non-homologous end-joining repair. The Science of the total environment 10 36526188
2021 Overexpression of a Pak Choi Gene, BcAS2, Causes Leaf Curvature in Arabidopsis thaliana. Genes 10 33467565
2018 Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit in Saccharomycescerevisiae. Genetics 10 30546002
2020 BCAS2, a protein enriched in advanced prostate cancer, interacts with NBS1 to enhance DNA double-strand break repair. British journal of cancer 9 32963349
2018 Identification of potential inhibitors against nuclear Dam1 complex subunit Ask1 of Candida albicans using virtual screening and MD simulations. Computational biology and chemistry 8 29346071
2016 Conditional Knockout of Breast Carcinoma Amplified Sequence 2 (BCAS2) in Mouse Forebrain Causes Dendritic Malformation via β-catenin. Scientific reports 8 27713508
2015 BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. The international journal of biochemistry & cell biology 8 26319152
2010 A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression. Biotechnology progress 8 20730753
2002 Dam1 is the right one: phosphoregulation of kinetochore biorientation. Developmental cell 7 12431367
2024 BCAS2 regulates oocyte meiotic prophase I by participating in mRNA alternative splicing. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 6 38085152
2021 Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I. Open biology 6 33529549
2020 Knockdown of long non-coding RNA plasmacytoma variant translocation 1 inhibits cell proliferation while promotes cell apoptosis via regulating miR-486-mediated CDK4 and BCAS2 in multiple myeloma. Irish journal of medical science 6 31900844
2012 Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast. Journal of cell science 6 22375062
2023 BCAS2 Participates in Insulin Synthesis and Secretion via mRNA Alternative Splicing in Mice. Endocrinology 5 37820033
2023 Molecular characterization, expression patterns and cellular localization of BCAS2 gene in male Hezuo pig. PeerJ 5 37901468
2019 Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast. Chromosoma 5 30903360
2023 BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing. Journal of ovarian research 4 37248466
2002 Mouse DAM1 regulates pro-apoptotic activity of BLK in mammary epithelial cells. Cancer letters 4 12406557
2016 The Nuclear Transport Factor Kap121 Is Required for Stability of the Dam1 Complex and Mitotic Kinetochore Bi-orientation. Cell reports 3 26947076
2025 Spindle integrity is regulated by a phospho-dependent interaction between the Ndc80 and Dam1 kinetochore complexes. PLoS genetics 2 40184422
2024 BCAS2 and hnRNPH1 orchestrate alternative splicing for DNA double-strand break repair and synapsis in meiotic prophase I. Cellular and molecular life sciences : CMLS 2 39520542
2023 Increased Sampling and Intracomplex Homologies Favor Vertical Over Horizontal Inheritance of the Dam1 Complex. Genome biology and evolution 2 36790109
2010 The Dad1 subunit of the yeast kinetochore Dam1 complex is an intrinsically disordered protein. Biochemical and biophysical research communications 2 20727855
2008 Production and initial characterization of Dad1p, a component of the Dam1-DASH kinetochore complex. PloS one 2 19065263
2025 BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus. eLife 1 40511787
2025 The RNA Binding Protein Bcas2 is Required for Antibody Class Switch in Activated-B Cells. Exploration (Beijing, China) 1 40585763
2024 Chromosome segregation: Mps1 and Dam1 battle to bind a shared interaction site at the kinetochore. Current biology : CB 1 38834024
2026 hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development. Nature communications 0 41680151
2025 BcAS2 Regulates Leaf Adaxial Polarity Development in Non-Heading Chinese Cabbage by Directly Activating BcPHB Transcription. Plants (Basel, Switzerland) 0 40284095
2025 Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation. PLoS genetics 0 40523001
2020 Structural view of the yeast Dam1 complex, a ring-shaped molecular coupler for the dynamic microtubule end. Essays in biochemistry 0 32579171