Affinage

ANAPC5

Anaphase-promoting complex subunit 5 · UniProt Q9UJX4

Length
755 aa
Mass
85.1 kDa
Annotated
2026-06-09
56 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/7 claims corpus-supported (86%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ANAPC5 (APC5) is a core scaffolding subunit of the anaphase-promoting complex/cyclosome (APC/C), the multisubunit E3 ubiquitin ligase that targets cell-cycle regulators for proteasomal degradation (PMID:9469815). Together with APC1 and APC4, APC5 builds the platform subcomplex that bridges the catalytic module (APC2/APC11/APC10) to the TPR co-activator-binding subunits; this subcomplex assembles polyubiquitin chains but cannot itself bind CDH1 or ubiquitinate substrates, marking its role as structural rather than catalytic (PMID:12956947, PMID:21307936). Structural work resolved the APC5 N-terminal α-helical fold and its contacts with APC4, and showed that the platform supports the coactivator-induced allosteric change required for UbcH10-dependent ubiquitination (PMID:26343760, PMID:27601667). Within the complex, APC5 directly binds substrates such as E2F1, mediating its K11-linked ubiquitination and degradation after S phase (PMID:22580462), and genetic studies across organisms reveal substrate-selective contributions: Drosophila IDA/APC5 is required for cyclin B degradation but not sister-chromatid separation (PMID:11870214), and C. elegans SUCH-1/APC5 governs SAC-dependent mitotic timing with a redundant paralog acting in meiosis (PMID:20944014, PMID:20944012). Beyond the APC/C, APC5 engages CBP/p300 to stimulate their acetyltransferase activity and transcription and to suppress E1A-mediated transformation (PMID:16319895), binds poly(A)-binding protein to repress IRES-mediated translation (PMID:15082755), and negatively regulates IL-17 signaling through association with IL-17 receptors and the deubiquitinase A20 (PMID:23922952). APC5 is also a target of viral subversion: HCMV pUL21a binds the APC/C and drives proteasomal degradation of APC5 (and APC4/APC1) to inactivate the complex (PMID:20686030, PMID:22792066). More recent work implicates ANAPC5-mediated ubiquitination of GPAA1 and EGFR in tumor immune evasion and macrophage polarization (PMID:41512182, PMID:40834712).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1998 High

    Established APC5 as a bona fide subunit of the APC/C, defining its membership in the cell-cycle ubiquitin ligase before any mechanistic role was known.

    Evidence Biochemical purification and cloning of human APC subunits

    PMID:9469815

    Open questions at the time
    • Did not assign a specific function to APC5 within the complex
    • No structural placement
  2. 2003 High

    Answered whether APC5 is catalytic or structural by reconstituting an APC1/APC4/APC5 subcomplex that builds ubiquitin chains but cannot bind CDH1 or ubiquitinate substrates, defining APC5 as a scaffold bridging the catalytic and co-activator-recruiting modules.

    Evidence Biochemical fractionation, subcomplex reconstitution, in vitro ubiquitination and CDH1 binding assays

    PMID:12956947

    Open questions at the time
    • No atomic-resolution view of contacts
    • Mechanism of coactivator-induced activation unresolved
  3. 2011 High

    Placed APC5 spatially within the holo-complex, showing it coordinates juxtaposition of the catalytic module and TPR subunits in a pseudo-atomic model.

    Evidence Cryo-EM, mass spectrometry and crystallographic docking of reconstituted APC/C

    PMID:21307936

    Open questions at the time
    • Limited resolution of APC5 internal architecture
  4. 2015 High

    Resolved the APC5 N-terminal fold and its APC4 contacts, refining the structural basis of platform assembly.

    Evidence Crystallography of Apc4 and Apc5N fitted into cryo-EM

    PMID:26343760

    Open questions at the time
    • Full-length APC5 structure not determined
    • Functional consequence of conformational changes untested
  5. 2016 High

    Defined how the APC5-containing platform contributes to catalysis by supporting the coactivator-induced allosteric change required for UbcH10-dependent ubiquitination.

    Evidence Crystal structure of Apc1 WD40, cryo-EM of APC/C-Cdh1, and in vitro ubiquitination assays

    PMID:27601667

    Open questions at the time
    • APC5-specific contribution not isolated from APC1/APC4
  6. 2005 High

    Revealed an APC/C-independent role by showing APC5 (with APC7) binds CBP/p300 to stimulate acetyltransferase activity, potentiate transcription, and suppress E1A-mediated transformation.

    Evidence Reciprocal Co-IP, in vitro acetyltransferase and reporter assays, RNAi, and transformation assays

    PMID:16319895

    Open questions at the time
    • Whether this occurs within or independent of assembled APC/C unclear
    • Physiological transcriptional targets not mapped
  7. 2004 Medium

    Identified APC5 as a translational regulator that binds PABP and represses IRES-mediated translation, expanding its function beyond ubiquitination.

    Evidence Yeast three-hybrid, Co-IP, sucrose gradient sedimentation, and IRES reporter assays

    PMID:15082755

    Open questions at the time
    • Single lab
    • Generality across IRES-containing mRNAs untested
    • Mechanism of translational repression unresolved
  8. 2012 Medium

    Showed APC5 directly binds E2F1 and is required for its K11-linked ubiquitination and S-phase-coupled degradation, identifying a specific substrate-recognition contribution.

    Evidence In vivo/in vitro Co-IP, GST pulldown mapping, K11-specific ubiquitination assays

    PMID:22580462

    Open questions at the time
    • Single lab
    • Whether APC5 is a dedicated substrate receptor or adaptor unclear
  9. 2013 Medium

    Identified APC5 as a negative regulator of IL-17 signaling through binding IL-17RA/RC and the deubiquitinase A20.

    Evidence Yeast two-hybrid, Co-IP, and siRNA knockdown with cytokine-stimulated gene expression

    PMID:23922952

    Open questions at the time
    • Single lab
    • Whether ubiquitin ligase activity is involved unclear
    • No in vivo validation
  10. 2010 Medium

    Demonstrated that APC5 is a target of viral attack, undergoing proteasomal degradation during HCMV infection coincident with APC/C disassembly.

    Evidence Immunoblotting, proteasome inhibition, viral mutant infections, phosphatase assays, MS

    PMID:20686030

    Open questions at the time
    • Viral effector not yet identified at this stage
    • Direct vs. indirect degradation unresolved
  11. 2012 High

    Identified HCMV pUL21a as the effector that binds the APC/C and is necessary and sufficient to degrade APC5/APC4, defining the mechanism of viral APC/C inactivation.

    Evidence Co-IP, pUL21a expression alone, point-mutant virus, proteasome inhibitor assays, proteomics

    PMID:22792066

    Open questions at the time
    • Ubiquitin ligase responsible for degradation not identified
  12. 2015 Medium

    Revealed co-regulation of platform subunits: loss of any single platform subunit (APC1/APC4/APC5) triggers co-degradation of all three, and pUL21a also targets APC1.

    Evidence siRNA knockdown of individual subunits, immunoblotting, UL21a expression

    PMID:25903336

    Open questions at the time
    • Single lab
    • Mechanism sensing platform integrity unknown
  13. 2013 Medium

    Defined cross-organism substrate roles: yeast Apc5 interacts with core subunits and targets Fob1 for G1-specific degradation linking APC5 to genomic stability and longevity.

    Evidence Yeast two-hybrid, protein stability assays, genetic epistasis, rDNA recombination assays (with prior subunit-interaction co-purification)

    PMID:12609981 PMID:24361936

    Open questions at the time
    • Single lab
    • Conservation of Fob1-like substrates in metazoans unknown
  14. 2009 High

    Showed fission yeast Atf1 binds and stimulates APC/C ubiquitylation of cyclin B and securin independent of its DNA-binding domain and suppresses apc5-1 arrest, revealing a regulatory protein acting through APC5.

    Evidence Genetic suppressor analysis, Co-IP, cell-free APC/C ubiquitination with purified Atf1

    PMID:19584054

    Open questions at the time
    • Whether contact is on APC5 itself or another subunit not pinpointed
    • Human ortholog relevance untested
  15. 2010 Medium

    Genetically distinguished APC5 functions in animal mitosis and meiosis, including SAC-dependent mitotic timing and redundant paralog roles in the germline.

    Evidence C. elegans allele characterization, time-lapse imaging, RNAi epistasis and co-depletion (with Drosophila ida mutant analysis)

    PMID:11870214 PMID:20944012 PMID:20944014

    Open questions at the time
    • Single-organism findings
    • Molecular basis of substrate selectivity unresolved
  16. 2024 Medium

    Showed opposing roles for ANAPC5 and ANAPC7 in modulating mitotic progression downstream of KIF18A.

    Evidence siRNA co-depletion in cell lines with flow-cytometric and microscopic mitotic-arrest quantification

    PMID:39677807 PMID:40596695

    Open questions at the time
    • Single lab
    • Mechanistic basis for opposing effects unknown
  17. 2025 Medium

    Linked ANAPC5 to immune regulation by showing its overexpression ubiquitinates and suppresses EGFR to drive M2 macrophage polarization via the EGFR/CD24 axis.

    Evidence Overexpression in vitro/in vivo, ubiquitination assays, EGFR inhibitor rescue, ALI mouse model

    PMID:40834712

    Open questions at the time
    • Single lab
    • Whether EGFR is a direct APC/C substrate unclear
  18. 2026 Medium

    Connected radiation-induced APC/C inhibition to immune evasion via loss of ANAPC5-mediated GPAA1 ubiquitination, stabilizing GPAA1 and enhancing CD24 surface display and phagocytosis resistance.

    Evidence ANAPC5 knockdown/overexpression, ubiquitination assays, CD24 flow cytometry, in vivo tumor models with immune cell depletion

    PMID:41512182

    Open questions at the time
    • Single lab
    • Direct vs. indirect ubiquitination of GPAA1 not fully established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How APC5's structural scaffolding role is mechanistically partitioned from its multiple moonlighting activities (transcription, translation, immune signaling, non-cell-cycle substrate targeting) remains unresolved.
  • No structural basis for direct substrate binding by APC5 within the assembled complex
  • Whether moonlighting functions require free vs. complex-bound APC5 untested
  • Human in vivo physiological relevance of non-mitotic roles largely undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 4 GO:0140096 catalytic activity, acting on a protein 3 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 1 GO:0005840 ribosome 1
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-74160 Gene expression (Transcription) 1
Partners
ANAPC1ANAPC4ANAPC7CREBBPE2F1EP300PABPTNFAIP3
Complex memberships
APC/C (anaphase-promoting complex/cyclosome)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 ANAPC5 (APC5) was identified as one of four previously uncharacterized human APC subunits (alongside APC2, APC4, APC7) and shown to be a core component of the anaphase-promoting complex, an eight-subunit E3 ubiquitin ligase responsible for targeting cell cycle regulators for degradation. Biochemical purification and cloning of human APC subunits Science High 9469815
2003 APC5, together with APC1 and APC4, forms a subcomplex that can assemble multiubiquitin chains but cannot bind CDH1 or ubiquitinate substrates. APC5 likely acts as a scaffold connecting the catalytic module (APC2/APC11) with the TPR subunits that recruit co-activators. Biochemical fractionation and reconstitution of human APC subcomplexes; in vitro ubiquitination assays; CDH1 binding assays Current Biology High 12956947
2011 Cryo-EM and mass spectrometry structural analysis of the APC/C places APC5 (along with APC1 and APC4) as a scaffolding subunit that coordinates the juxtaposition of the catalytic module (APC2, APC11, APC10) and TPR subunits, providing a pseudo-atomic model for APC/C organization. Recombinant reconstitution of holo-APC/C; electron microscopy; mass spectrometry; crystallographic docking Nature High 21307936
2015 Crystal structures of Apc4 and the N-terminal domain of Apc5 (Apc5N) were determined; Apc5N adopts an α-helical fold, and in the context of the APC/C, shows small conformational changes and contacts Apc4 to order regions disordered in the crystal. Protein crystallography; fitting into cryo-EM map Journal of Molecular Biology High 26343760
2016 APC5 (as part of the APC/C platform with APC1, APC4, and APC15) supports the coactivator-induced allosteric conformational change required for UbcH10-dependent ubiquitination; the platform structurally coordinates the catalytic and substrate-recognition modules. Crystal structure of Apc1 WD40 domain; cryo-EM of APC/C-Cdh1 with Apc1 WD40 deletion; in vitro ubiquitination assays PNAS High 27601667
2005 APC5 and APC7 directly interact with transcriptional coactivators CBP and p300 through protein-protein interaction domains evolutionarily conserved in adenovirus E1A; this interaction stimulates intrinsic CBP/p300 acetyltransferase activity and potentiates CBP/p300-dependent transcription. Co-immunoprecipitation; in vitro acetyltransferase assays; reporter gene transcription assays; RNAi knockdown of CBP Nature High 16319895
2005 APC5 and APC7 suppress E1A-mediated cellular transformation in a CBP/p300-dependent manner, indicating these subunits are functionally targeted during cellular transformation. Transformation assay with APC5/APC7 expression in CBP/p300-proficient vs. -deficient contexts Nature Medium 16319895
2004 Apc5 binds poly(A) binding protein (PABP) and represses IRES-mediated translation of PDGF-2 mRNA; overexpression of Apc5 counteracts PABP-enhanced IRES activity, and Apc5 co-sediments with the ribosomal fraction, indicating a role outside the APC/C in translational regulation. Yeast three-hybrid screen; co-immunoprecipitation; sucrose gradient sedimentation; IRES reporter assays; overexpression in differentiated cells Molecular and Cellular Biology Medium 15082755
2012 APC5 binds E2F1 directly (confirmed by in vivo and in vitro Co-IP/GST pulldown) and is essential for E2F1 ubiquitination by APC/C-Cdh1, which targets E2F1 for K11-linked ubiquitin chain-mediated proteasomal degradation after S phase. Co-immunoprecipitation in vivo and in vitro; GST pulldown mapping; ubiquitination assays with K11-specific ubiquitin Cell Cycle Medium 22580462
2013 ANAPC5 binds IL-17RA and IL-17RC and associates with the deubiquitinase A20 (TNFAIP3); siRNA-mediated knockdown of ANAPC5 enhances IL-17-induced gene expression, demonstrating that ANAPC5 acts as a negative regulator of IL-17 signaling. Yeast two-hybrid screen; co-immunoprecipitation; siRNA knockdown with cytokine-stimulated gene expression readout PLoS ONE Medium 23922952
2010 During HCMV infection, APC5 and APC4 subunits undergo proteasome-dependent degradation, which is temporally associated with disassembly of the APC/C core complex and requires viral early gene expression (not immediate early genes alone), leading to APC/C inactivation. Immunoblotting; proteasome inhibitor treatment; UV-inactivated and deletion mutant virus infections; phosphatase assays; mass spectrometry Journal of Virology Medium 20686030
2012 HCMV protein pUL21a physically binds the APC/C and is necessary and sufficient to induce proteasome-dependent degradation of APC5 and APC4, thereby disrupting APC/C integrity; residues P109-R110 of pUL21a are critical for APC binding and APC5/APC4 degradation. Co-immunoprecipitation; expression of pUL21a alone; point mutant virus construction; proteasome inhibitor assays; proteomics PLoS Pathogens High 22792066
2015 HCMV protein UL21a also targets APC1 for degradation (in addition to APC4 and APC5); furthermore, depletion of any single platform subunit (APC1, APC4, or APC5) or APC8 triggers coordinated co-degradation of all three platform subunits, revealing a cellular mechanism for platform subunit co-regulation. siRNA knockdown of individual APC subunits; immunoblotting; UL21a expression in uninfected cells Journal of Virology Medium 25903336
2002 In Drosophila, IDA (the APC5 homolog) is required for APC/C-dependent degradation of cyclin B but not for degradation of substrates controlling sister-chromatid separation; ida mutants display high mitotic index with aneuploid, overcondensed chromosomes, defining a subfunction-specific role for APC5 within the APC/C. Genetic mutant analysis; cytological examination of mitotic stages; immunostaining for APC/C substrate levels Journal of Cell Science Medium 11870214
2003 Yeast Apc5 physically interacts with Cdc23 and Apc1 in co-expression in vitro transcription/translation; yeast two-hybrid and co-purification experiments confirmed Mnd2 and Swm1 interact with Apc5 and Cdc23 as core APC subunits. In vitro transcription/translation co-expression; mass spectrometry identification; co-purification with epitope-tagged subunits Journal of Biological Chemistry Medium 12609981
2002 In yeast, APC5 (RMC1) is required for in vitro chromatin assembly; apc5 mutants display UV sensitivity, plasmid loss, G2/M accumulation, and genetic interactions with apc9Δ, apc10Δ, and cdc26Δ, linking APC5 function to chromatin metabolism and APC complex integrity. In vitro chromatin assembly screen; genetic epistasis analysis; phenotypic characterization of ts mutants Genetics Medium 12399376
2013 The APC (via Apc5) targets Fob1 for degradation specifically in G1; Fob1 is unstable in G1, stabilized in apc5(CA) and proteasome mutants, and Fob1 deletion suppresses apc5(CA) cell cycle and rDNA recombination defects, placing APC5-dependent Fob1 degradation in a pathway linking APC to replicative longevity and genomic stability. Yeast two-hybrid (Apc5 as bait, Fob1 as prey); protein stability assays; genetic epistasis; rDNA recombination assays Genetics Medium 24361936
2009 Fission yeast Atf1 physically binds the APC/C in vivo; purified Atf1 stimulates ubiquitylation of cyclin B and securin by the APC/C in a cell-free system, independent of Atf1's DNA-binding (bZIP) domain; atf1+ is a dose-dependent suppressor of apc5-1 mitotic arrest. Genetic suppressor analysis; co-immunoprecipitation; cell-free APC/C ubiquitination assay with purified Atf1 Journal of Biological Chemistry High 19584054
2010 In C. elegans, SUCH-1/APC5 is required for normal mitotic timing; a novel such-1(t1668) allele causes prolonged mitosis in embryos with monopolar spindles, and this delay is SAC-dependent (rescued by mdf-1/MAD1 or mdf-2/MAD2 inactivation), suggesting the APC/C negatively regulates the SAC. Genetic characterization of new allele; time-lapse imaging; epistasis with SAC components by RNAi Genetics Medium 20944014
2010 C. elegans has two APC5 paralogs (such-1 and gfi-3) that are coexpressed in the germline; co-depletion (but not individual depletion) causes meiotic arrest, demonstrating functionally redundant roles for APC5 paralogs in meiosis. RNAi co-depletion; germline expression analysis; phenotypic characterization of meiotic arrest Genetics Medium 20944012
2024 Co-depletion of ANAPC5 in cell lines exacerbates KIF18A-depletion-induced mitotic arrest, whereas co-depletion of ANAPC7 partially rescues this arrest, indicating opposing roles for ANAPC5 and ANAPC7 in modulating mitotic progression downstream of KIF18A. siRNA co-depletion in cell lines; mitotic arrest quantification by flow cytometry and microscopy Scientific Reports / bioRxiv Medium 39677807 40596695
2026 Radiation inhibits the APC/C complex, reducing ANAPC5-mediated ubiquitination of GPAA1 (a catalytic subunit of GPI transamidase); the resulting accumulation of GPAA1 enhances GPI anchoring and CD24 membrane localization, promoting phagocytosis resistance and immune evasion in irradiated tumor cells. ANAPC5 knockdown/overexpression; ubiquitination assays; flow cytometry for CD24 surface expression; in vivo tumor models with T cell and macrophage depletion Cancer Research Medium 41512182
2025 ANAPC5 overexpression in macrophages induces ubiquitination and suppression of EGFR, thereby promoting M2 macrophage polarization over M1; this effect is mediated through the EGFR/CD24 axis, and ANAPC5 overexpression reduces lung inflammation in an LPS-induced ALI mouse model. ANAPC5 overexpression in vitro and in vivo; ubiquitination assays; EGFR inhibitor rescue; ALI mouse model with pathological and cytokine analysis Cellular Immunology Medium 40834712

Source papers

Stage 0 corpus · 56 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 Differential immune system DNA methylation and cytokine regulation in post-traumatic stress disorder. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 261 21714072
1998 Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science (New York, N.Y.) 211 9469815
2003 TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. Current biology : CB 172 12956947
2011 Structural basis for the subunit assembly of the anaphase-promoting complex. Nature 137 21307936
2009 FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. Journal of proteome research 86 19344176
2005 The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression. Nature 84 16319895
2012 The yeast forkhead transcription factors fkh1 and fkh2 regulate lifespan and stress response together with the anaphase-promoting complex. PLoS genetics 59 22438832
2003 Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. The Journal of biological chemistry 51 12609981
2002 Phenotypic characterization of Drosophila ida mutants: defining the role of APC5 in cell cycle progression. Journal of cell science 40 11870214
2012 Regulation of E2F1 by APC/C Cdh1 via K11 linkage-specific ubiquitin chain formation. Cell cycle (Georgetown, Tex.) 37 22580462
2004 A functional analysis reveals dependence on the anaphase-promoting complex for prolonged life span in yeast. Genetics 34 15514051
2010 Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1. Journal of virology 32 20686030
2012 Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a. PLoS pathogens 28 22792066
2007 Induction of heme oxygenase-1 by cobalt protoporphyrin enhances the antitumour effect of bortezomib in adult T-cell leukaemia cells. British journal of cancer 25 17895889
2009 The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast. The Journal of biological chemistry 24 19584054
2007 Suppressors of spindle checkpoint defect (such) mutants identify new mdf-1/MAD1 interactors in Caenorhabditis elegans. Genetics 24 17237515
2002 The ubiquitin-dependent targeting pathway in Saccharomyces cerevisiae plays a critical role in multiple chromatin assembly regulatory steps. Genetics 24 12399376
2013 Validation of reference genes for the determination of platelet transcript level in healthy individuals and in patients with the history of myocardial infarction. International journal of molecular sciences 20 23389042
2013 The anaphase-promoting complex protein 5 (AnapC5) associates with A20 and inhibits IL-17-mediated signal transduction. PloS one 20 23922952
2021 System Biology-Guided Chemical Proteomics to Discover Protein Targets of Monoethylhexyl Phthalate in Regulating Cell Cycle. Environmental science & technology 19 33459556
2010 The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression. Eukaryotic cell 19 20709786
2017 Co‑expression of Axin and APC gene fragments inhibits colorectal cancer cell growth via regulation of the Wnt signaling pathway. Molecular medicine reports 18 28731177
2011 Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast. Cell division 18 21651791
2016 WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity. Proceedings of the National Academy of Sciences of the United States of America 17 27601667
2015 Studies on the Contribution of Human Cytomegalovirus UL21a and UL97 to Viral Growth and Inactivation of the Anaphase-Promoting Complex/Cyclosome (APC/C) E3 Ubiquitin Ligase Reveal a Unique Cellular Mechanism for Downmodulation of the APC/C Subunits APC1, APC4, and APC5. Journal of virology 17 25903336
2013 The anaphase promoting complex regulates yeast lifespan and rDNA stability by targeting Fob1 for degradation. Genetics 16 24361936
2004 The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation. Molecular and cellular biology 16 15082755
2005 Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae. Eukaryotic cell 12 15821127
2015 Differential Expression of Cell Cycle Regulators During Hyperplastic and Hypertrophic Growth of Broiler Subcutaneous Adipose Tissue. Lipids 11 26017028
1998 New junction models for alternate-strand triple-helix formation. Chemistry & biology 11 9862797
2010 Mutual antagonism between the anaphase promoting complex and the spindle assembly checkpoint contributes to mitotic timing in Caenorhabditis elegans. Genetics 10 20944014
2017 A novel APC promoter 1B deletion shows a founder effect in Italian patients with classical familial adenomatous polyposis phenotype. Genes, chromosomes & cancer 9 28791770
2015 Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain. Journal of molecular biology 9 26343760
2010 Functional redundancy of paralogs of an anaphase promoting complex/cyclosome subunit in Caenorhabditis elegans meiosis. Genetics 8 20944012
2024 Unraveling the molecular pathogenesis of Type 2 Diabetes and its impact on female infertility: A bioinformatics and systems biology approach. Computers in biology and medicine 7 39116715
2020 The role of CAMKK2 polymorphisms in HIV-associated sensory neuropathy in South Africans. Journal of the neurological sciences 7 32585444
2019 Polymorphisms in CAMKK2 associate with susceptibility to sensory neuropathy in HIV patients treated without stavudine. Journal of neurovirology 7 31309408
2006 Cross-talk between APC/C and CBP/p300. Cancer biology & therapy 7 16861917
2021 Morpho-molecular and mating-type locus diversity of Ustilaginoidea virens: an incitant of false smut of rice from Southern parts of India. Journal of applied microbiology 5 33772985
2003 Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae. Biological procedures online 5 14615812
2001 Expression of anaphase-promoting complex 5 in balloon angioplasty-injured rat carotid arteries and mitogen-stimulated human vascular smooth muscle cells. Biochemical and biophysical research communications 4 11401522
2025 Utility of a Large Series of B-Cell Precursor Acute Lymphoblastic Leukemia Cell Lines as a Model System. Cancer medicine 3 40022573
2021 Using Budding Yeast to Identify Molecules That Block Cancer Cell 'Mitotic Slippage' Only in the Presence of Mitotic Poisons. International journal of molecular sciences 3 34360748
2025 Two cGMP-dependent protein kinases have opposing effects on molt-inhibiting hormone regulation of Y-organ ecdysteroidogenesis. The Journal of experimental biology 2 39850985
2026 Radiation-Enhanced CD24 Membrane Trafficking via GPI Anchoring Mediates Antitumor Immune Evasion. Cancer research 1 41512182
2025 Exploring the role of circRNA-miRNA-mRNA interactions in cervical cancer progression: insights into HPV status and potential therapeutic approaches. Journal of applied genetics 1 40531429
2024 Anapc5 and Anapc7 as genetic modifiers of KIF18A function in fertility and mitotic progression. bioRxiv : the preprint server for biology 1 39677807
2010 [Effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of beta-catenin in HT-29 cells]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 1 20197613
2026 Single-Cell and Mendelian Randomization Analyses Identify Key Genes Common to COVID-19 and Multiple Sclerosis. Viral immunology 0 41841545
2026 Dracocephalum tanguticum Maxim Polysaccharide Ameliorate Autoimmune Hepatitis via Regulating TNF and IL-17 Signaling Pathways. Food science & nutrition 0 42016259
2026 ​​Deletion of Ybx1 results in germ cell hypoplasia in mouse embryos. The Journal of reproduction and development 0 42219335
2025 Therapeutic targeting of alternative pathway and C5 but not C5a protects from disease development in a preclinical model of autoimmune blistering dermatosis. Frontiers in immunology 0 40370446
2025 Anapc5 and Anapc7 as genetic modifiers of KIF18A function in fertility and mitotic progression. Scientific reports 0 40596695
2025 ANAPC5 mitigates acute lung injury through regulating macrophage M1/M2 polarization via the EGFR/CD24 axis. Cellular immunology 0 40834712
2025 PTTG1 as a common promising target for PCOS, Ovarian Cancer, and Major Depressive Disorder patients. Computational biology and chemistry 0 40925190
2018 [Identifying the Importance of MT-3 Expression for Neuroblastoma Cells]. Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti 0 29808689

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