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ANAPC1

Anaphase-promoting complex subunit 1 · UniProt Q9H1A4

Round 2 corrected
Length
1944 aa
Mass
216.5 kDa
Annotated
2026-04-28
62 papers in source corpus 11 papers cited in narrative 11 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ANAPC1 encodes the largest scaffolding subunit of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle progression through targeted degradation of mitotic cyclins and other substrates (PMID:17689521, PMID:25903336). Its N-terminal WD40 domain mediates coactivator (Cdh1/Cdc20)-induced allosteric activation of the APC/C catalytic module, enabling UbcH10-dependent ubiquitin chain initiation on substrates (PMID:27601667). Two disordered regulatory loops (loop300 and loop500) serve as phospho-regulated switches: CDK1/Plk1 phosphorylation and PP2A-B55/B56-mediated dephosphorylation of these loops control Cdc20 co-activator loading and thereby couple APC/C activity to cell cycle oscillation (PMID:31825153, PMID:34291488, PMID:38678563). Loss-of-function mutations in ANAPC1 cause Rothmund-Thomson syndrome type 1, characterized by reduced ANAPC1 protein, prolonged interphase, and increased cataract incidence in a heterozygous mouse model (PMID:31303264).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2001 Medium

    Full-length sequencing and chromosomal mapping of human ANAPC1 established its identity as the largest APC/C subunit and showed that APC/C subunits are co-expressed at stable stoichiometric ratios across tissues, consistent with a constitutive multi-subunit complex.

    Evidence cDNA sequencing, chromosomal mapping, Northern blot/RT-PCR across human tissues

    PMID:11179667

    Open questions at the time
    • Single-lab characterization without functional assays
    • No structural information on APC1 domains
    • Tissue-level expression data lacks single-cell resolution
  2. 2003 High

    Mass spectrometry-based phospho-mapping revealed that mitotic CDK1 phosphorylation of APC/C is concentrated on Apc1 and TPR subunits, and that this phosphorylation is sufficient to promote Cdc20 binding and APC/C activation—establishing phospho-regulation as the primary mechanism for mitotic APC/C activation.

    Evidence Mass spectrometry of immunopurified APC/C, in vitro Cdk1/Plk1 kinase assays, phospho-specific immunofluorescence, Plk1 depletion

    PMID:14657031

    Open questions at the time
    • Specific Apc1 phospho-sites responsible for Cdc20 binding were not individually assigned
    • Phosphatase(s) counteracting CDK1 on APC/C not yet identified
  3. 2007 High

    Genetic analysis of Drosophila apc1 (shattered) mutants demonstrated that APC1 is required for G1 establishment and mitotic progression through degradation of Cyclin A and String/Cdc25, providing in vivo epistatic evidence placing APC1 upstream of key cell cycle substrates.

    Evidence Drosophila genetic mutant analysis, immunostaining, genetic dosage suppression of CycA and string

    PMID:17689521

    Open questions at the time
    • Invertebrate model; direct mapping to mammalian APC1 regulatory domains not performed
    • No biochemical reconstitution of Drosophila APC/C activity
  4. 2015 High

    Discovery that HCMV protein UL21a targets APC1 for proteasomal degradation, and that loss of any single platform subunit (APC1, APC4, APC5) triggers coordinated co-degradation of the others, revealed both a viral immune-evasion strategy and an intrinsic quality-control mechanism for the APC/C platform subcomplex.

    Evidence siRNA knockdowns of individual platform subunits, viral protein overexpression, Western blot, substrate accumulation assays

    PMID:25903336

    Open questions at the time
    • E3 ligase mediating cellular co-degradation not identified
    • Functional consequence of partial APC/C platform loss on specific substrates not quantified
  5. 2016 High

    Crystal structure of the Apc1 WD40 domain and cryo-EM of WD40-deleted APC/C revealed that Apc1-WD40 is required for coactivator-induced repositioning of the catalytic module to an active conformation that exposes the UbcH10-binding site—mechanistically separating chain initiation (UbcH10-dependent, WD40-dependent) from chain elongation (Ube2S-dependent, WD40-independent).

    Evidence 2.2 Å crystal structure, cryo-EM of ΔWD40 APC/C-Cdh1, in vitro ubiquitination with UbcH10 and Ube2S

    PMID:27601667

    Open questions at the time
    • How WD40 domain communicates allosteric signal to catalytic module at atomic resolution remains incomplete
    • Role of WD40 in Cdc20-mediated (vs Cdh1-mediated) activation not separately tested
  6. 2019 High

    Identification of ANAPC1 mutations as the cause of Rothmund-Thomson syndrome type 1 in seven families connected APC/C deficiency to a human developmental disorder, with patient cells showing reduced ANAPC1 protein and prolonged interphase, and heterozygous knockout mice recapitulating the cataract phenotype.

    Evidence Exome/targeted sequencing across 7 families, RT-PCR splice analysis, Western blot of patient fibroblasts, cell cycle analysis, Anapc1+/- mouse model

    PMID:31303264

    Open questions at the time
    • How partial APC/C loss causes the specific RTS1 spectrum (poikiloderma, cataracts) rather than broader cell cycle failure is unknown
    • No rescue experiment restoring ANAPC1 in patient cells
  7. 2019 High

    Reconstitution showed that Apc1-loop500 directly recruits PP2A-B56, which preferentially dephosphorylates Cdc20 over Apc1's inhibitory domain to promote Cdc20 loading—resolving how a phosphatase paradoxically activates APC/C during mitosis when most APC/C phosphorylation is activating.

    Evidence Xenopus egg extract reconstitution, Apc1-loop500 mutagenesis, co-immunoprecipitation, in vitro ubiquitination, non-phosphorylatable Cdc20 mutant rescue

    PMID:31825153

    Open questions at the time
    • Quantitative kinetics of PP2A-B56 substrate selectivity on APC/C not measured
    • Whether loop500-B56 interaction is conserved in human cells not directly tested
  8. 2021 High

    Identification of a phosphorylation-dependent feedback loop in which Plk1 and PP2A-B56 compete for binding to Apc1-loop500 revealed that Plk1 binding is controlled by Apc3-loop phosphorylation—demonstrating inter-subunit phospho-communication within the APC/C and explaining how Plk1 and PP2A-B56 sequentially regulate APC/C activation timing.

    Evidence Xenopus egg extract reconstitution, site-directed mutagenesis of Apc1-loop500 and Apc3-loop, phospho-specific binding assays, kinase/phosphatase manipulation

    PMID:34291488

    Open questions at the time
    • Structural basis for Plk1-loop500 vs B56-loop500 competitive binding not resolved
    • In vivo validation in mammalian cells lacking
  9. 2021 Medium

    HIV-1 Vpr was found to target APC1 for DCAF1-dependent proteasomal degradation, representing a second independent viral strategy (after HCMV UL21a) to disable the APC/C, though the functional advantage for viral replication remains unclear.

    Evidence BioID proximity labeling, co-immunoprecipitation, Western blot, proteasome inhibitor rescue, mutant Vpr analysis

    PMID:34011540

    Open questions at the time
    • APC1 degradation did not affect G2 arrest or HIV replication in macrophages, so the biological purpose is unresolved
    • Single-lab finding without independent replication
  10. 2024 High

    Discovery that CDK1 phosphorylation of a second disordered loop, Apc1-loop300, and its dephosphorylation by PP2A-B55 is a primary node of the CDK-PP2A-B55 cell cycle oscillator—dephosphorylation of loop300 blocks Cdc20 recruitment and delays Cyclin B degradation, identifying the APC/C itself as a key PP2A-B55 substrate for mitotic exit control.

    Evidence Xenopus egg extract, Greatwall kinase depletion, pEnsa inhibitor, Apc1-300L deletion mutants, Cdc20 binding assays

    PMID:38678563

    Open questions at the time
    • Whether loop300 and loop500 regulation are additive or sequential in vivo is not distinguished
    • Mammalian in vivo validation of loop300 function not performed

Open questions

Synthesis pass · forward-looking unresolved questions
  • The integrated structural and biochemical logic by which the two regulatory loops (loop300, loop500) coordinate with the WD40 domain and multiple kinases/phosphatases to produce the sharp activation switch of APC/C during mitotic progression remains to be resolved at atomic resolution in a single structural framework.
  • No full-length structure of phosphorylated human APC/C capturing loop300 and loop500 conformations simultaneously
  • In vivo kinetic measurements of loop phosphorylation/dephosphorylation dynamics lacking in mammalian cells
  • Mechanism by which partial APC1 loss produces the tissue-specific phenotypes of Rothmund-Thomson syndrome type 1 is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 4 GO:0005198 structural molecule activity 3
Localization
GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-1640170 Cell Cycle 6 R-HSA-392499 Metabolism of proteins 2 R-HSA-1643685 Disease 1
Partners
ANAPC15ANAPC4ANAPC5CDC20CDH1PLK1PP2A-B55PP2A-B56
Complex memberships
APC/C

Evidence

Reading pass · 11 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 Human APC1 (the largest subunit of the APC/C) was fully sequenced, its chromosomal location mapped, and intron-exon boundaries analyzed. APC/C subunits including APC1 are expressed at fairly constant levels relative to each other across most tissues, consistent with their function as part of a stable complex. Full-length cDNA sequencing, chromosomal mapping, Northern blot/RT-PCR expression analysis Gene Medium 11179667
2003 APC/C phosphorylation by Cdk1 in mitosis generates at least 32 mitosis-specific phosphorylation sites clustered in Apc1 and TPR subunits; phosphorylation of APC (but not Plk1 activity alone) is sufficient for increased Cdc20 binding and APC activation, and APC phosphorylation is initiated in prophase during nuclear uptake of cyclin B1. Mass spectrometry identification of phospho-sites on immunopurified APC/C; in vitro kinase assays with Cdk1/Plk1; immunofluorescence with phospho-specific antibodies; Plk1 depletion experiments The EMBO journal High 14657031
2007 In Drosophila, the shattered (shtd) gene encodes Apc1, the largest subunit of the APC/C. Loss of Apc1 causes failure to establish G1 arrest and defects in progression through mitosis, accompanied by accumulation of Cyclin A and String (Cdc25) proteins. Genetic reduction of CycA or string dosage suppresses the shtd phenotype, placing Apc1 upstream of these substrates in cell cycle control. Genetic mutant analysis, immunostaining for cell cycle markers, genetic epistasis (dosage suppression) Developmental biology High 17689521
2015 Human cytomegalovirus (HCMV) protein UL21a induces the proteasomal degradation of APC1 (in addition to APC4 and APC5). UL21a is necessary and sufficient to degrade APC1. Furthermore, depletion of any single platform subunit (APC1, APC4, or APC5) or of APC8 in uninfected cells leads to co-degradation of all three platform subunits, revealing a cellular mechanism for coordinated downregulation of the APC/C platform subcomplex. siRNA knockdown, overexpression of viral proteins, Western blotting, APC/C substrate accumulation assays Journal of virology High 25903336
2016 The WD40 domain of Apc1 (Apc1(WD40)) is required for the coactivator (Cdh1)-induced allosteric conformational change of the APC/C that stimulates UbcH10-dependent ubiquitination of substrates (chain initiation). Deletion of Apc1(WD40) abolishes UbcH10-dependent ubiquitination but does not impair Ube2S-dependent ubiquitin chain elongation. Crystal structure of the N-terminal WD40 domain of human Apc1 was determined at 2.2 Å resolution; cryo-EM of APC/C-Cdh1 with Apc1(WD40) deleted showed the catalytic module locked in an inactive conformation with inaccessible UbcH10-binding site and loss of Apc15 density. Crystal structure (2.2 Å), cryo-EM, WD40 deletion mutant APC/C reconstitution, in vitro ubiquitination assays with UbcH10 and Ube2S Proceedings of the National Academy of Sciences of the United States of America High 27601667
2019 Mutations in ANAPC1 cause Rothmund-Thomson syndrome type 1 (RTS1). A deep intronic splicing mutation activates a 95 bp pseudoexon, causing premature termination codons, nonsense-mediated decay, decreased ANAPC1 protein levels, and prolongation of interphase in patient fibroblasts. Mice heterozygous for an Anapc1 knockout show increased cataract incidence, linking APC/C deficiency to the RTS1 phenotype. Exome/targeted sequencing, RT-PCR splice analysis, Western blot for ANAPC1 protein in fibroblasts, cell cycle analysis of patient fibroblasts, mouse knockout model American journal of human genetics High 31303264
2019 A disordered loop domain of Apc1 (Apc1-loop500) directly binds the B56 regulatory subunit of PP2A and promotes Cdc20 loading onto the APC/C. Mutations in Apc1-loop500 that abolish B56 binding decrease Cdc20 loading and APC/C-dependent ubiquitylation in Xenopus egg extract reconstitution. PP2A-B56 preferentially dephosphorylates Cdc20 over the Apc1 inhibitory domain, facilitating APC/C-Cdc20 complex formation in mitosis. APC/C reconstitution in Xenopus egg extracts, mutagenesis of Apc1-loop500, co-immunoprecipitation, in vitro ubiquitination assays, non-phosphorylatable Cdc20 mutant rescue EMBO reports High 31825153
2021 HIV-1 Vpr mediates degradation of APC1 via DCAF1 recruitment and the proteasome. Vpr forms a complex with APC1, and the APC/C coactivators Cdh1 and Cdc20 are associated with these complexes. APC1 degradation is a conserved feature of several primary Vpr variants from transmitted/founder HIV-1 viruses, though it does not impact Vpr-mediated G2 arrest or HIV replication in macrophages. BioID proximity labeling, co-immunoprecipitation, Western blot for APC1 degradation, proteasome inhibitor rescue, mutant Vpr analysis Journal of virology Medium 34011540
2021 Plx1 (polo-like kinase) directly binds the Apc1-loop500 domain in a phosphorylation-dependent manner and promotes APC/C-Cdc20 formation via Apc3 phosphorylation. Upon phosphorylation of loop residue T532, PP2A-B56 is recruited to Apc1-loop500 and differentially promotes dissociation of Plx1 through dephosphorylation of Plx1-binding sites, while stable Plx1 binding prevents PP2A-B56 recruitment and prematurely activates APC/C. The phosphorylation status of Apc1-loop500 is controlled by distant Apc3-loop phosphorylation, revealing a phosphorylation-dependent feedback within the APC/C. Xenopus egg extract reconstitution, site-directed mutagenesis, phospho-specific binding assays, kinase/phosphatase manipulation The EMBO journal High 34291488
2024 Phospho-regulation of Apc1-loop300 (Apc1-300L) by CDK1 and PP2A-B55 is pivotal for cell cycle oscillation. Premature PP2A-B55 activation (via Greatwall kinase depletion) leads to Apc1-300L dephosphorylation, stalling APC/C activity and delaying Cyclin B degradation; this effect is counteracted by B55-specific inhibitor pEnsa or by removing Apc1-300L. Dephosphorylation of Apc1-300L specifically inhibits Cdc20 recruitment to the APC/C, identifying APC/C as a primary substrate of the CDK-PP2A-B55 partnership for cell cycle oscillation. Xenopus egg extract assays, Greatwall kinase depletion, pEnsa inhibitor, Apc1-300L deletion mutants, Cdc20 binding assays across cell cycle stages Cell reports High 38678563
2024 Cryo-EM comparison of S. cerevisiae and human APC/C reveals that, unlike human APC/C, yeast apo-APC/C has the catalytic module already positioned to bind E2 without requiring coactivator-induced conformational change. Furthermore, no evidence for a phospho-regulatable auto-inhibitory segment of Apc1 (equivalent to the human Apc1 inhibitory loop that sterically blocks the CDC20 C-box binding site on APC8 in unphosphorylated human APC/C) is found in yeast, demonstrating species-specific regulatory differences in the Apc1-mediated auto-inhibition mechanism. Cryo-EM structures of yeast apo-APC/C, APC/C-CDH1-substrate ternary complex, and phosphorylated apo-APC/C; comparative structural analysis with human APC/C bioRxivpreprint Medium bio_10.1101_2024.06.19.599685

Source papers

Stage 0 corpus · 62 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2004 Large-scale characterization of HeLa cell nuclear phosphoproteins. Proceedings of the National Academy of Sciences of the United States of America 1159 15302935
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 Genome-wide meta-analysis identifies 56 bone mineral density loci and reveals 14 loci associated with risk of fracture. Nature genetics 958 22504420
2009 A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene. Cell 843 19490893
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2001 Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2. The Journal of cell biology 726 11535616
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2011 Global landscape of HIV-human protein complexes. Nature 593 22190034
2008 Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. Cell 442 18485873
2011 SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity. Cancer cell 441 22014574
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2013 Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions. Science signaling 383 24255178
2001 Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. The Journal of cell biology 372 11285280
2007 Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Molecular cell 367 17643375
2000 Mitotic regulation of the APC activator proteins CDC20 and CDH1. Molecular biology of the cell 363 10793135
2000 Characterization of vertebrate cohesin complexes and their regulation in prophase. The Journal of cell biology 358 11076961
2003 Mitotic regulation of the human anaphase-promoting complex by phosphorylation. The EMBO journal 341 14657031
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2011 Cubism and the cell cycle: the many faces of the APC/C. Nature reviews. Molecular cell biology 323 21633387
2003 Shortstop recruits EB1/APC1 and promotes microtubule assembly at the muscle-tendon junction. Current biology : CB 94 12842007
2012 Non-autonomous crosstalk between the Jak/Stat and Egfr pathways mediates Apc1-driven intestinal stem cell hyperplasia in the Drosophila adult midgut. Development (Cambridge, England) 88 23172913
2002 Drosophila Apc1 and Apc2 regulate Wingless transduction throughout development. Development (Cambridge, England) 84 11923210
2002 Drosophila APC2 and APC1 have overlapping roles in the larval brain despite their distinct intracellular localizations. Developmental biology 57 12297097
2002 Drosophila APC2 and APC1 play overlapping roles in wingless signaling in the embryo and imaginal discs. Developmental biology 57 12297098
2019 Mutations in ANAPC1, Encoding a Scaffold Subunit of the Anaphase-Promoting Complex, Cause Rothmund-Thomson Syndrome Type 1. American journal of human genetics 46 31303264
2005 Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. The Journal of biological chemistry 29 15994309
2008 Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 27 18622497
2019 Sequence variation at ANAPC1 accounts for 24% of the variability in corneal endothelial cell density. Nature communications 24 30894546
2007 Mutation of the Apc1 homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis. Developmental biology 22 17689521
2001 Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 22 11179667
2019 PP2A-B56 binds to Apc1 and promotes Cdc20 association with the APC/C ubiquitin ligase in mitosis. EMBO reports 17 31825153
2016 WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity. Proceedings of the National Academy of Sciences of the United States of America 17 27601667
2015 Studies on the Contribution of Human Cytomegalovirus UL21a and UL97 to Viral Growth and Inactivation of the Anaphase-Promoting Complex/Cyclosome (APC/C) E3 Ubiquitin Ligase Reveal a Unique Cellular Mechanism for Downmodulation of the APC/C Subunits APC1, APC4, and APC5. Journal of virology 17 25903336
2012 ANAPC1 and SLCO3A1 are associated with nicotine dependence: meta-analysis of genome-wide association studies. Drug and alcohol dependence 15 22377092
1998 The APC1 concept of type I diabetes. Autoimmunity 15 9609135
2009 Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival. Developmental biology 11 19397905
2009 Apc1-mediated antagonism of Wnt/beta-catenin signaling is required for retino-tectal pathfinding in the zebrafish. Zebrafish 10 19292673
2021 Dynamic regulation of mitotic ubiquitin ligase APC/C by coordinated Plx1 kinase and PP2A phosphatase action on a flexible Apc1 loop. The EMBO journal 9 34291488
1996 A urochordate putative homolog of human EB1, the protein which binds APC1. Cancer letters 9 9020915
2021 Human Immunodeficiency Virus Type 1 Vpr Mediates Degradation of APC1, a Scaffolding Component of the Anaphase-Promoting Complex/Cyclosome. Journal of virology 6 34011540
2017 Oral Contraceptive Use May Modulate Global Genomic DNA Methylation and Promoter Methylation of APC1 and ESR1. Asian Pacific journal of cancer prevention : APJCP 6 28950679
2005 The Aspergillus nidulans sldI(RAD50) gene interacts with bimE(APC1), a homologue of an anaphase-promoting complex subunit. Molecular microbiology 6 15948962
2021 Rothmund-Thomson syndrome type 1 caused by biallelic ANAPC1 gene mutations. Skin health and disease 5 35664819
2024 [SNG2], a prion form of Cut4/Apc1, confers non-Mendelian inheritance of heterochromatin silencing defect in fission yeast. Nucleic acids research 3 39565210
2024 CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop300. Cell reports 2 38678563
2022 Antibody for Serine 65 Phosphorylated Ubiquitin Identifies PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1. Molecules (Basel, Switzerland) 2 35956818
2025 Elevated expression of ANAPC1 in lung squamous cell carcinoma: clinical implications and mechanisms. Future science OA 1 40139913
2026 mGem: Cut4/Apc1 and its prion form at the cross roads of cell cycle regulation, heterochromatin organization, RNAi, stress response, and evolution. mBio 0 41910327
2026 Overexpression of ANAPC1 Affects the Cell Cycle Pathway to Promote the Progression of Lung Adenocarcinoma. Combinatorial chemistry & high throughput screening 0 42002993
2025 Small Molecules Identified by an In Silico Docking Screen Targeting Anaphase-Promoting Complex/Cyclosome Subunit 1 (APC1) Potentiate Paclitaxel-Induced Breast Cancer Cell Death. Molecules (Basel, Switzerland) 0 40005207
2025 Exploring the potential function of high expression of ANAPC1 in regulating ubiquitination in hepatocellular carcinoma. World journal of gastrointestinal oncology 0 40487972