Affinage

URI1

Unconventional prefoldin RPB5 interactor 1 · UniProt O94763

Length
535 aa
Mass
59.8 kDa
Annotated
2026-06-10
95 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/7 claims corpus-supported (86%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

URI1 (RMP/RPB5-mediating protein) is a prefoldin-family co-chaperone and nutrient/growth-factor-responsive scaffold that links signaling to transcription, phosphatase control, and cell survival (PMID:14615539, PMID:17936702). As a transcriptional co-repressor it binds RPB5, the subunit shared by all three RNA polymerases, through a central domain that overlaps the TFIIB- and HBx-binding surfaces, and additionally engages TFIIB and both subunits of TFIIF (RAP30/RAP74) via its C-terminal D5 domain to suppress activated transcription (PMID:9819440, PMID:10712776, PMID:12737519). URI1 assembles with the R2TP/prefoldin-like complex in the nucleus, regulates RPB5 stability, and shuttles between nucleus and cytoplasm through CRM1-dependent export sensitive to RNA polymerase II stalling (PMID:23667685). A central regulatory module is its control of protein phosphatase 1: URI1 sequesters PP1γ in inactive complexes, and S6K1-mediated phosphorylation at Ser-371 (or PKA phosphorylation under glucose deprivation) disassembles these complexes to govern S6K1–BAD survival signaling and an OGT–c-MYC axis (PMID:17936702, PMID:27505673). Through these activities URI1 acts as an oncogene that sustains tumor-cell survival, suppresses p53 surveillance via a TRIM28–MDM2 axis to reprogram SCD1-driven lipid metabolism, and represses retrotransposons by recruiting PP2A to KAP1 (PMID:21397856, PMID:37805657, PMID:27780869). URI1 also maintains genome integrity and tissue homeostasis: its loss provokes DNA damage and p53-dependent apoptosis in germline and tumor cells, and it protects intestinal stem cells by restraining β-catenin–c-MYC signaling (PMID:16436622, PMID:18412953, PMID:31147493). In pancreatic β cells URI1 loss drives estrogen-receptor–dependent DNMT1 expression and Pdx1 promoter hypermethylation, silencing PDX1 (PMID:33205075).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1998 High

    Established URI1's founding biochemical identity as an RPB5-binding protein that negatively modulates RNA polymerase II, defining it as a transcriptional co-repressor.

    Evidence Far-Western screening, in vitro binding with domain-deletion mutants, and luciferase reporter assays in human cells

    PMID:9819440

    Open questions at the time
    • Did not place URI1 in a broader cellular signaling pathway
    • Did not resolve whether repression occurs on chromatin in vivo
  2. 2000 Medium

    Defined the molecular basis of repression by showing URI1 competes with HBx for the TFIIB d10 domain, with TFIIB overexpression rescuing repression.

    Evidence In vitro competition pull-down and CAT reporter assays in COS-1 cells

    PMID:10712776

    Open questions at the time
    • Overlaps mechanistically with the RPB5-binding work and is from a single lab
    • Did not test endogenous gene targets
  3. 2003 High

    Extended the co-repressor mechanism to TFIIF and embedded URI1 in nutrient signaling by linking it to prefoldins and TOR-controlled transcription.

    Evidence TFIIF pull-down/Far-Western with D5 domain mapping (Cell Research); co-IP of prefoldin/RPB5 complexes and rapamycin epistasis in yeast and human (Science)

    PMID:12737519 PMID:14615539

    Open questions at the time
    • Did not identify the kinase coupling nutrient status to URI1
    • Did not resolve the structure of the URI1–prefoldin complex
  4. 2006 High

    Demonstrated an in vivo requirement for URI1 in genome integrity by showing germline loss causes DNA breaks and p53-dependent apoptosis.

    Evidence RNAi and mutant allele of C. elegans uri-1 with TUNEL, HUS-1::GFP foci, and p53 genetic epistasis

    PMID:16436622

    Open questions at the time
    • Did not define the molecular source of endogenous DNA damage
    • Did not connect the phenotype to a specific URI1 biochemical activity
  5. 2007 High

    Identified URI1 as an S6K1 substrate whose Ser-371 phosphorylation toggles PP1γ sequestration, mechanistically coupling growth-factor signaling to the apoptotic threshold.

    Evidence In vitro kinase assay, mitochondrial fractionation, phospho-specific antibodies, S371A/D mutants, and PP1γ activity assays

    PMID:17936702

    Open questions at the time
    • Did not establish whether the PP1γ module operates in non-mitochondrial compartments
    • Did not address transcriptional consequences of the switch
  6. 2008 High

    Refined the phosphatase interaction by showing conserved PP1α selectivity and dual cytoplasmic/chromatin localization, linking URI1 to active RNAPII while confirming its genome-stability role across organisms.

    Evidence PP1α/β discriminating binding assays, ChIP of active RNAPII, and Drosophila uri loss-of-function with DNA damage readouts

    PMID:18412953

    Open questions at the time
    • Did not reconcile PP1α selectivity in flies with mammalian PP1γ-focused models
    • Did not define how chromatin and cytoplasmic pools are partitioned
  7. 2011 High

    Established URI1 as an amplified oncogene and a context-dependent transcriptional regulator, sustaining survival via PP1γ sequestration in ovarian cancer and repressing AR target genes in prostate cancer.

    Evidence FISH copy-number, siRNA with viability/cisplatin assays and URI/PP1γ co-IP (Cancer Cell); ChIP, microarray, and Art-27 co-IP in prostate cells (MCB)

    PMID:21397856 PMID:21730289

    Open questions at the time
    • Did not unify the survival-signaling and transcriptional-repression roles
    • Did not define how androgen-induced URI1 phosphorylation alters chromatin occupancy
  8. 2013 Medium

    Mapped URI1's nuclear interactome to the R2TP/prefoldin-like complex and PDRG1 and defined CRM1-dependent, transcription-state-sensitive shuttling controlling RPB5 stability.

    Evidence MS-based nuclear proteomics, co-IP, and leptomycin B/α-amanitin/actinomycin-D treatments

    PMID:23667685

    Open questions at the time
    • Functional consequences of identified phospho/acetyl sites untested
    • Single-lab interactome without reciprocal validation for all partners
  9. 2014 High

    Connected URI1 to metabolic genome protection and tumor-promoting transcription, showing it suppresses NAD+ biosynthesis to cause DNA damage and activates NF-κB/RPB5–driven IL-6 to expand cancer stem cells.

    Evidence Hepatocyte URI transgenic/knockout mice with RNA-seq, NAD+ metabolomics and nicotinamide riboside rescue (Cancer Cell); p65/RPB5 co-IP, IL-6 reporter and metastasis models (Oncogene); p65 co-IP and STAT3/IL-6 assays in myeloma (Cell Death Dis); yeast Bud27–RSC/RNAPII CTD co-IP and ChIP (NAR)

    PMID:24625985 PMID:24704835 PMID:25081216 PMID:25453901

    Open questions at the time
    • Did not reconcile URI1 acting as both a repressor and an NF-κB co-activator
    • Relationship between metabolic NAD+ control and the PP1γ module unresolved
  10. 2016 High

    Defined nutrient- and phosphatase-coupled mechanisms downstream of URI1: a glucose-sensing URI1/PP1γ/OGT–c-MYC switch, PP2A recruitment to KAP1 for retrotransposon silencing, and URI1-complex suppression of p53 surveillance.

    Evidence URI/PP1γ/OGT co-IP with S371A knock-in mouse and OGT/O-GlcNAc assays (Cancer Cell); URI/KAP1/PP2A co-IP, phosphatase assay and transposon microarray (JBC); conditional knockdown with p53 reporter and xenograft (Oncotarget)

    PMID:27105489 PMID:27505673 PMID:27780869

    Open questions at the time
    • Did not establish how the same Ser-371 switch is interpreted by S6K1 versus PKA in different tissues
    • STAP1-containing URI1C composition not fully defined biochemically
  11. 2019 High

    Showed URI1 governs stem-cell radiotolerance in vivo by restraining β-catenin–induced c-MYC to limit DNA damage in intestinal label-retaining cells.

    Evidence Intestine-specific URI transgenic and heterozygous knockout mice, irradiation, β-catenin reporter, and γH2AX/label-retaining-cell imaging

    PMID:31147493

    Open questions at the time
    • Did not define the direct biochemical link between URI1 and β-catenin
    • Did not test whether the PP1γ/OGT module participates in stem-cell protection
  12. 2020 High

    Revealed a tissue-specific epigenetic mechanism in which URI1 loss drives ER-dependent DNMT1 expression and Pdx1 promoter hypermethylation, silencing PDX1 and predisposing to diabetes.

    Evidence Pancreas-specific URI knockout and CVB4 infection mouse models, bisulfite sequencing, and procainamide/PDX1-overexpression rescue

    PMID:33205075

    Open questions at the time
    • Did not define how URI1 normally restrains ER nuclear translocation
    • Did not connect β-cell PDX1 control to URI1's transcription/PP1γ roles
  13. 2023 High

    Established a TRIM28–MDM2–p53–SCD1 axis by which URI1 promotes p53 degradation to reprogram lipid metabolism and confer ferroptosis resistance.

    Evidence URI/TRIM28/MDM2 co-IP, ubiquitination assay, p53 ChIP at SCD1, lipid profiling, and organoid/xenograft models

    PMID:37805657

    Open questions at the time
    • Did not determine whether prefoldin/co-chaperone activity is required for TRIM28 binding
    • Relationship to NAD+-mediated p53/DNA-damage effects not integrated
  14. 2022 Medium

    Extended URI1 function to immune-cell biology by linking macrophage RMP to M1/M2 polarization and angiogenesis after myocardial infarction.

    Evidence Macrophage-specific RMP knockout, BMDM adoptive transfer, MI model, and HSP90/p38 pathway markers

    PMID:36092156

    Open questions at the time
    • Single-lab study without biochemical demonstration of URI1–HSP90 interaction
    • Connection to URI1's transcriptional/phosphatase roles unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How URI1's distinct activities — prefoldin co-chaperone, RPB5/TFII transcriptional repressor, PP1γ/PP2A scaffold, and p53/β-catenin/ER modulator — are coordinated within a single protein, and what determines which axis dominates in a given cell type, remains unresolved.
  • No structural model integrating the co-chaperone, RPB5-binding, and phosphatase-scaffolding surfaces
  • Switching logic between repressor and NF-κB co-activator roles undefined
  • No unified model linking the Ser-371 phospho-switch to tissue-specific outcomes

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 3 GO:0140110 transcription regulator activity 3 GO:0044183 protein folding chaperone 2
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2 GO:0005829 cytosol 2 GO:0005739 mitochondrion 1
Pathway
R-HSA-1430728 Metabolism 3 R-HSA-1643685 Disease 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-162582 Signal Transduction 2
Partners
KAP1OGTPDRG1PP1ΓRELA (P65)RPB5TFIIBTRIM28
Complex memberships
R2TP/prefoldin-like complexURI1-KAP1-PP2A complexURI1/PP1γ/OGT complexURI1C (URI1-STAP1 prefoldin chaperone complex)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 URI (RMP/NNX3) forms complexes with small-molecular-weight prefoldins and with RPB5, a shared subunit of all three RNA polymerases. Both yeast and human orthologs are targets of TOR kinase-dependent nutrient signaling and participate in TOR-controlled gene expression programs, establishing URI as a component linking nutrient availability to transcription. Immunoprecipitation/co-IP, yeast and human functional genetics, rapamycin treatment Science High 14615539
1998 RMP (URI1) was identified as an RPB5-binding protein that negatively modulates RNA polymerase II function. RMP binds RPB5 (but not HBx or TBP) in vitro; the central domain of RMP mediates RPB5 binding overlapping the TFIIB- and HBx-binding sites of RPB5. Overexpression of RMP (but not an RPB5-binding-deficient mutant) inhibits HBx transactivation and VP16-dependent transcriptional activation. Far-Western blot screening, in vitro binding assay, co-immunoprecipitation, luciferase reporter assay, domain-deletion mutagenesis Molecular and cellular biology High 9819440
2000 RMP (URI1) competes with HBx for binding to the d10 domain of TFIIB (TF2B); RMP and HBx mutually disrupt each other's interaction with TFIIB in vitro, and overexpression of TFIIB rescues RMP-mediated repression of HBx transactivation, indicating that competitive TFIIB binding underlies RMP's transcriptional co-repressor function. In vitro pull-down (protein-protein binding competition assay), CAT reporter assay, co-expression in COS-1 cells Zhonghua gan zang bing za zhi Medium 10712776
2003 RMP (URI1) also suppresses activated transcription through direct interaction with both subunits of general transcription factor IIF (RAP30 and RAP74). The C-terminal D5 domain of RMP (118 aa) is sufficient for TFIIF binding and is required for transcriptional repression; deletion of D5 abolishes both TFIIF binding and suppression of Gal-VP16-activated transcription. In vitro pull-down, Far-Western analysis, co-immunoprecipitation in COS1 cells, domain-deletion mutagenesis, luciferase reporter assay Cell research High 12737519
2007 URI is a mitochondrial substrate of S6K1. In growth-factor-deprived or rapamycin-treated cells, URI forms stable complexes with PP1γ at mitochondria, inhibiting PP1γ activity. Growth factor stimulation induces S6K1-mediated phosphorylation of URI at serine 371, disassembling URI/PP1γ complexes, activating PP1γ-dependent dephosphorylation of S6K1 and BAD, thereby reducing S6K1 survival signaling and lowering the apoptotic threshold. Co-immunoprecipitation, subcellular fractionation, in vitro kinase assay, phospho-specific antibodies, S371A/D mutants, rapamycin treatment, PP1γ activity assay Molecular cell High 17936702
2006 C. elegans uri-1 is essential for germ cell proliferation and genome integrity. URI-1-deficient cells exhibit cell cycle arrest, DNA breaks (TUNEL-positive and HUS-1::GFP foci), and p53-dependent germline apoptosis, placing URI-1 in a pathway required to suppress endogenous genotoxic DNA damage in mitotic and meiotic cells. RNAi loss-of-function, uri-1(lf) allele, TUNEL staining, HUS-1::GFP foci imaging, genetic epistasis with p53 pathway Development High 16436622
2008 Drosophila Uri binds PP1α with much higher affinity than PP1β, and this PP1α selectivity is conserved in humans. Most Uri is cytoplasmic, but some associates with active RNAPII on chromatin. Loss of uri in Drosophila causes lethality, transcriptional defects, reduced germline cell viability and differentiation, and nuclear DNA damage accumulation. PP1 binding/affinity assay (discriminating PP1α vs PP1β), chromatin immunoprecipitation (active RNAPII), uri loss-of-function allele generation, TUNEL/γH2AX for DNA damage BMC molecular biology High 18412953
2011 URI is amplified/overexpressed in ovarian cancer and functions as an oncogene required for tumor cell survival by constitutively sequestering PP1γ in inactive complexes, thereby sustaining S6K1-BAD survival signaling under growth-factor-limiting conditions and conferring resistance to cisplatin. FISH/copy-number analysis, siRNA knockdown, cell viability assays, cisplatin sensitivity, co-immunoprecipitation of URI/PP1γ complexes Cancer cell High 21397856
2011 URI is a transcriptional repressor of androgen receptor (AR)-mediated transcription in prostate cancer cells. URI is phosphorylated upon androgen treatment; URI binds and regulates the AR corepressor Art-27; URI occupies chromatin at AR target gene promoters prior to hormone-dependent AR recruitment; depletion of URI increases AR occupancy and NKX-3.1 expression while decreasing Art-27 recruitment. Co-immunoprecipitation, chromatin immunoprecipitation (ChIP), genome-wide expression profiling (microarray), siRNA knockdown, phosphorylation detection by western blot Molecular and cellular biology High 21730289
2013 URI interacts with all components of the R2TP/prefoldin-like complex in the nucleus, binds and regulates RPB5 protein stability and transcription. URI nuclear/cytoplasmic shuttling is sensitive to RNA pol II stalling (α-amanitin, actinomycin-D) and is mediated by the CRM1 nuclear export pathway (blocked by leptomycin B). URI also interacts with PDRG1 and stabilizes it. Novel URI phosphorylation and acetylation sites were identified. Mass spectrometry-based nuclear proteomics, co-immunoprecipitation, leptomycin B/α-amanitin/actinomycin-D treatment, western blot for protein stability PloS one Medium 23667685
2014 URI inhibits aryl hydrocarbon receptor (AhR)- and estrogen receptor (ER)-mediated transcription of enzymes in the L-tryptophan/kynurenine/NAD+ biosynthesis pathway, thereby reducing intracellular NAD+ levels, causing DNA damage at early stages of hepatocarcinogenesis. Restoring NAD+ with nicotinamide riboside prevents URI-driven DNA damage and tumor formation in mouse models. Hepatocyte-specific URI transgenic and conditional knockout mice (genetically engineered mouse models), RNA-seq/transcriptome analysis, NAD+ metabolite quantification, nicotinamide riboside rescue experiment, DEN-induced HCC model Cancer cell High 25453901
2014 RMP (URI1) promotes HCC venous metastasis and tumor-initiating cell self-renewal by interacting with NF-κB p65 and RPB5 to activate IL-6 transcription, thereby expanding cancer stem cell populations and inducing epithelial-mesenchymal transition. Co-immunoprecipitation (RMP with p65/RPB5), reporter assays for IL-6 transcription, IL-6 neutralization assay, in vitro and in vivo metastasis models, shRNA knockdown Oncogene Medium 24704835
2014 URI modulates STAT3 activity in multiple myeloma cells through regulation of IL-6 transcription via interaction with NFκB p65. shRNA knockdown, co-immunoprecipitation, STAT3 phosphorylation assay, IL-6 reporter/quantification Cell death & disease Medium 24625985
2016 URI, PP1γ, and OGT form a functional complex that is maintained by glucose. Glucose deprivation activates PKA, which phosphorylates URI at Ser-371, releasing PP1γ and causing URI-mediated OGT inhibition. Reduced OGT activity lowers O-GlcNAcylation and promotes c-MYC degradation. In the presence of glucose, PP1γ-bound URI maintains high OGT activity and c-MYC levels. Mice expressing non-phosphorylatable URI (S371A) show constitutively high OGT and c-MYC, accelerating liver tumorigenesis. Co-immunoprecipitation (URI/PP1γ/OGT complex), phospho-specific URI-S371 antibody, S371A knock-in mouse, OGT activity assay, O-GlcNAcylation western blot, c-MYC protein stability assay, glucose deprivation/PKA inhibitor experiments Cancer cell High 27505673
2016 URI interacts with KAP1 and recruits PP2A phosphatase, reducing KAP1 phosphorylation. The URI-KAP1-PP2A complex mediates retrotransposon (LINE-1/L1PA2) repression in prostate cancer cells; URI knockdown selectively increases LINE-1 and L1PA2 transcription. Co-immunoprecipitation (URI with KAP1 and PP2A), PP2A phosphatase activity assay, KAP1 phosphorylation western blot, microarray analysis of transposable elements, siRNA knockdown The Journal of biological chemistry High 27780869
2016 In URI1-dependent colorectal cancer cells, URI1 deficiency (but not in URI1-independent cells) causes non-genotoxic p53 activation and p53-dependent apoptosis, implicating the URI1 prefoldin chaperone complex (URI1C, comprising URI1 and partner STAP1) in suppression of p53 surveillance. siRNA/conditional knockdown, p53 reporter assay, apoptosis assay, p53 target gene expression, xenograft tumor model with conditional URI1 depletion Oncotarget Medium 27105489
2019 URI overexpression in intestinal crypt protects mice from radiation-induced gastrointestinal syndrome (GIS) by inhibiting β-catenin in stem cell-like label-retaining (LR) cells. URI reduction activates β-catenin-induced c-MYC expression, causing DNA damage and radiosensitization of LR cells. URI thus maintains the radiotolerant state of intestinal LR stem cells required for organ regeneration. Intestine-specific URI transgenic and URI heterozygous knockout mice, irradiation model, β-catenin reporter assay, c-MYC expression analysis, BrdU/label-retaining cell imaging, DNA damage markers (γH2AX) Science High 31147493
2020 CVB4 viral infection and genetic URI ablation in mouse pancreas both cause loss of PDX1 in β cells. Mechanistically, URI loss triggers estrogen receptor nuclear translocation, leading to DNMT1 expression, which induces Pdx1 promoter hypermethylation and silencing. Demethylating agent procainamide (DNMT1 inhibitor) restores PDX1 expression and protects against diabetes in pancreatic URI-depleted mice. Pancreas-specific URI knockout mice, CVB4 infection model in human islet-engrafted mice, estrogen receptor nuclear translocation imaging, DNMT1 expression western blot, Pdx1 promoter methylation bisulfite sequencing, procainamide rescue experiment, PDX1-overexpression rescue in URI-OE diabetic mice Cell reports. Medicine High 33205075
2014 The yeast URI orthologue Bud27 associates with RNA pol II phosphorylated forms (CTD-Ser5P and CTD-Ser2P) and with the Sth1 subunit of the chromatin remodeling complex RSC, mediating RSC association with RNA pol II; its absence affects RNA pol II occupancy of transcribed genes. In addition to contributing to Rpb5 folding, Bud27 modulates RNA pol II transcription elongation. Co-immunoprecipitation (Bud27 with RNA pol II CTD forms and RSC/Sth1), ChIP analysis of RNA pol II occupancy, bud27 deletion strain Nucleic acids research Medium 25081216
2017 RMP (URI1) promotes EMT in HCC by activating NF-κB, which directly induces expression of COP9 signalosome subunit CSN2, which in turn represses Snail degradation, causing Snail accumulation and EMT/metastasis. Co-immunoprecipitation (RMP/p65), NF-κB reporter, CSN2 promoter luciferase assay, Snail stability assay (cycloheximide chase), shRNA knockdown, in vivo pulmonary metastasis mouse model, IHC of human HCC tissues Oncotarget Medium 28423737
2023 URI directly interacts with TRIM28 and promotes p53 ubiquitination and degradation in a TRIM28-MDM2-dependent manner. High URI suppresses p53, which normally represses SCD1 transcription; consequently, URI overexpression elevates SCD1 and reprograms lipid metabolism to confer resistance to TKI-induced ferroptosis in p53-wild-type HCC. Co-immunoprecipitation (URI/TRIM28/MDM2), ubiquitination assay, p53 ChIP at SCD1 promoter, SCD1 promoter luciferase reporter, lipid metabolism profiling, patient-derived organoids, xenograft models Nature communications High 37805657
2022 Macrophage RMP regulates M1/M2 polarization after myocardial infarction through the HSP90-p38 signaling pathway; macrophage-specific RMP knockout promotes M1 polarization and inhibits angiogenesis, while RMP overexpression promotes M2 polarization and angiogenesis. Macrophage-specific RMP knockout (LysM-Cre), bone marrow-derived macrophage transfer, MI mouse model, immunoblotting and immunofluorescence for polarization markers and HSP90/p38 pathway Oxidative medicine and cellular longevity Medium 36092156
2019 RMP/URI inhibits both intrinsic (cisplatin-induced) and extrinsic (TRAIL-induced) apoptosis via distinct mechanisms: in intrinsic apoptosis, RMP promotes Bcl-xl expression and activates NF-κB/p65 through ATM phosphorylation; in extrinsic apoptosis, RMP suppresses p53 transcription and expression, and forced p53 expression offsets this inhibitory effect. Flow cytometry (apoptosis rate), western blot (Bcl-xl, p65, pATM, p53), overexpression and knockdown, p53 rescue experiment International journal of biological sciences Medium 31754340

Source papers

Stage 0 corpus · 95 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage. Cancer cell 175 25453901
2003 Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI. Science (New York, N.Y.) 152 14615539
1996 Controlled modulation of BBB permeability using the bradykinin agonist, RMP-7. Experimental neurology 109 8912895
1995 Pathway across blood-brain barrier opened by the bradykinin agonist, RMP-7. Brain research 104 8821743
2007 S6K1-mediated disassembly of mitochondrial URI/PP1gamma complexes activates a negative feedback program that counters S6K1 survival signaling. Molecular cell 96 17936702
1998 RMP, a novel RNA polymerase II subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein. Molecular and cellular biology 90 9819440
1996 Permeability of the blood brain barrier by the bradykinin agonist, RMP-7: evidence for a sensitive, auto-regulated, receptor-mediated system. Immunopharmacology 90 8856161
1996 Unlocking the blood-brain barrier: a role for RMP-7 in brain tumor therapy. Experimental neurology 85 8812155
2016 Regulation of OGT by URI in Response to Glucose Confers c-MYC-Dependent Survival Mechanisms. Cancer cell 83 27505673
2023 URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers. Nature communications 71 37805657
1992 Gonococcal porin vaccine evaluation: comparison of Por proteosomes, liposomes, and blebs isolated from rmp deletion mutants. The Journal of infectious diseases 68 1323620
2011 URI is an oncogene amplified in ovarian cancer cells and is required for their survival. Cancer cell 67 21397856
2019 URI is required to maintain intestinal architecture during ionizing radiation. Science (New York, N.Y.) 66 31147493
2017 Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin. International journal of nanomedicine 63 28435263
2013 Red cell-derived microparticles (RMP) as haemostatic agent. Thrombosis and haemostasis 61 24030707
1994 The bradykinin analog RMP-7 increases intracellular free calcium levels in rat brain microvascular endothelial cells. The Journal of pharmacology and experimental therapeutics 61 7965719
2013 Analysis of URI nuclear interaction with RPB5 and components of the R2TP/prefoldin-like complex. PloS one 54 23667685
1993 Antibody to Rmp (outer membrane protein 3) increases susceptibility to gonococcal infection. The Journal of clinical investigation 54 8423230
2006 URI-1 is required for DNA stability in C. elegans. Development (Cambridge, England) 53 16436622
1999 Functional activities and epitope specificity of human and murine antibodies against the class 4 outer membrane protein (Rmp) of Neisseria meningitidis. Infection and immunity 53 10024570
1999 Enhanced delivery of carboplatin into brain tumours with intravenous Cereport (RMP-7): dramatic differences and insight gained from dosing parameters. British journal of cancer 52 10362103
2012 Increased circulating red cell microparticles (RMP) and platelet microparticles (PMP) in immune thrombocytopenic purpura. Thrombosis research 48 23245653
1998 Central analgesic actions of loperamide following transient permeation of the blood brain barrier with Cereport (RMP-7). Brain research 41 9729419
2014 RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription. Oncogene 40 24704835
2000 Intravenous cereport (RMP-7) modifies topographic uptake profile of carboplatin within rat glioma and brain surrounding tumor, elevates platinum levels, and enhances survival. The Journal of pharmacology and experimental therapeutics 39 10869391
2021 Triptolide decreases rheumatoid arthritis fibroblast-like synoviocyte proliferation, invasion, inflammation and presents a therapeutic effect in collagen-induced arthritis rats via inactivating lncRNA RP11-83J16.1 mediated URI1 and β-catenin signaling. International immunopharmacology 38 34358861
2000 Intravenous cereport (RMP-7) enhances delivery of hydrophilic chemotherapeutics and increases survival in rats with metastatic tumors in the brain. Pharmaceutical research 38 11145226
2011 Regulation of androgen receptor-mediated transcription by RPB5 binding protein URI/RMP. Molecular and cellular biology 33 21730289
2008 Drosophila Uri, a PP1alpha binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity. BMC molecular biology 33 18412953
1995 Chromosome mapping of Rmp-4, a gonad-dependent gene encoding host resistance to mousepox. Journal of virology 33 7474114
2012 Rapid and accurate detection of RMP- and INH- resistant Mycobacterium tuberculosis in spinal tuberculosis specimens by CapitalBio™ DNA microarray: a prospective validation study. BMC infectious diseases 31 23151186
1998 Intravenous RMP-7 increases delivery of ganciclovir into rat brain tumors and enhances the effects of herpes simplex virus thymidine kinase gene therapy. Human gene therapy 31 9607410
1999 Cereport (RMP-7) increases the permeability of human brain microvascular endothelial cell monolayers. Pharmaceutical research 30 10496650
2015 URI expression in cervical cancer cells is associated with higher invasion capacity and resistance to cisplatin. American journal of cancer research 29 26101702
2003 Interaction with general transcription factor IIF (TFIIF) is required for the suppression of activated transcription by RPB5-mediating protein (RMP). Cell research 29 12737519
2014 URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription. Cell death & disease 28 24625985
2023 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of aldosterone in human serum and plasma. Clinical chemistry and laboratory medicine 27 36952682
2018 The Expression of MicroRNA-598 Inhibits Ovarian Cancer Cell Proliferation and Metastasis by Targeting URI. Molecular therapy oncolytics 26 30662936
2016 URI Regulates KAP1 Phosphorylation and Transcriptional Repression via PP2A Phosphatase in Prostate Cancer Cells. The Journal of biological chemistry 26 27780869
1999 Selective delivery of herpes virus vectors to experimental brain tumors using RMP-7. Cancer gene therapy 26 10078959
2014 The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic acids research 25 25081216
2013 Expression analysis of URI/RMP gene in endometrioid adenocarcinoma by tissue microarray immunohistochemistry. International journal of clinical and experimental pathology 23 24228101
1999 Cereport (RMP-7) increases carboplatin levels in brain tumors after pretreatment with dexamethasone. Neuro-oncology 23 11550318
2020 Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing. Cell reports. Medicine 22 33205075
2018 HOTAIR promotes myocardial fibrosis through regulating URI1 expression via Wnt pathway. European review for medical and pharmacological sciences 21 30402865
2014 Characterization of the 19q12 amplification including CCNE1 and URI in different epithelial ovarian cancer subtypes. Experimental and molecular pathology 21 25527175
1998 Intra-arterial administration of carboplatin and the blood brain barrier permeabilizing agent, RMP-7: a toxicologic evaluation in swine. Journal of neuro-oncology 21 9525816
2014 The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP. International journal of biological sciences 19 25516716
2025 Genomic and functional analysis of rmp locus variants in Klebsiella pneumoniae. Genome medicine 18 40205597
2004 The effect of RMP-7 and its derivative on transporting Evans blue liposomes into the brain. Drug delivery 18 15742555
2002 Intracarotid RMP-7 enhanced indocyanine green staining of tumors in a rat glioma model. Journal of neuro-oncology 18 12061728
1992 Serial backcross analysis of genetic resistance to mousepox, using marker loci for Rmp-2 and Rmp-3. Journal of virology 18 1433507
2017 RMP promotes epithelial-mesenchymal transition through NF-κB/CSN2/Snail pathway in hepatocellular carcinoma. Oncotarget 17 28423737
1999 Pharmacological characterization of RMP-7, a novel bradykinin agonist in smooth muscle. Immunopharmacology 17 10614991
2021 URI1 suppresses irradiation-induced reactive oxygen species (ROS) by activating autophagy in hepatocellular carcinoma cells. International journal of biological sciences 16 34421352
2017 Detection of CCNE1/URI (19q12) amplification by in situ hybridisation is common in high grade and type II endometrial cancer. Oncotarget 16 27582547
2016 Colorectal cancer cells display chaperone dependency for the unconventional prefoldin URI1. Oncotarget 16 27105489
2013 Association of IL-2RA and IL-2RB genes with erosive status in early rheumatoid arthritis patients (ESPOIR and RMP cohorts). Joint bone spine 16 24200909
2019 RMP/URI inhibits both intrinsic and extrinsic apoptosis through different signaling pathways. International journal of biological sciences 15 31754340
2018 The Multiple Functions of the PAQosome: An R2TP- and URI1 Prefoldin-Based Chaperone Complex. Advances in experimental medicine and biology 15 30484152
2018 Roles and Functions of the Unconventional Prefoldin URI. Advances in experimental medicine and biology 13 30484155
2023 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of gabapentin in human serum and plasma. Clinical chemistry and laboratory medicine 12 36689915
2019 HBx and c-MYC Cooperate to Induce URI1 Expression in HBV-Related Hepatocellular Carcinoma. International journal of molecular sciences 12 31739577
2016 URI prevents potassium dichromate-induced oxidative stress and cell death in gastric cancer cells. American journal of translational research 12 28078011
2013 RMP plays distinct roles in the proliferation of hepatocellular carcinoma cells and normal hepatic cells. International journal of biological sciences 12 23847445
1997 Enhanced cytokines delivery and intercellular adhesion molecule 1 (ICAM-1) expression in glioma by intracarotid infusion of bradykinin analog, RMP-7. Neurological research 12 9329027
2015 RMP predicts survival and adjuvant TACE response in hepatocellular carcinoma. Oncotarget 11 25605019
2016 URI promotes gastric cancer cell motility, survival, and resistance to adriamycin in vitro. American journal of cancer research 10 27429854
2010 Antimutagenic; differentiation-inducing; and antioxidative effects of fragrant ingredients in Katsura-uri (Japanese pickling melon; Cucumis melo var. conomon). Mutation research 9 20801232
2014 Antibodies with higher bactericidal activity induced by a Neisseria gonorrhoeae Rmp deletion mutant strain. PloS one 8 24594737
2012 Replication-Associated Mutational Pressure (RMP) Governs Strand-Biased Compositional Asymmetry (SCA) and Gene Organization in Animal Mitochondrial Genomes. Current genomics 8 22942673
2023 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of lamotrigine in human serum and plasma. Clinical chemistry and laboratory medicine 7 36785916
2023 Age-adjusted high-dose chemotherapy followed by autologous stem cell transplantation or conventional chemotherapy with R-MP as first-line treatment in elderly primary CNS lymphoma patients - the randomized phase III PRIMA-CNS trial. BMC cancer 7 37596517
2017 URI promotes the migration and invasion of human cervical cancer cells potentially via upregulation of vimentin expression. American journal of translational research 7 28670391
2008 3-Methylthiopropionic acid ethyl ester, isolated from Katsura-uri (Japanese pickling melon, Cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. Journal of agricultural and food chemistry 7 18426216
2024 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of primidone in human serum and plasma. Clinical chemistry and laboratory medicine 6 38549258
2023 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of methotrexate in human serum and plasma. Clinical chemistry and laboratory medicine 6 36788118
2023 Silence of URI in gastric cancer cells promotes cisplatin-induced DNA damage and apoptosis. American journal of cancer research 6 37034221
2018 Role of the Unconventional Prefoldin Proteins URI and UXT in Transcription Regulation. Advances in experimental medicine and biology 6 30484154
2000 [Study of HBV X protein and RMP, an RPB5 mediate protein competitively interacting with general transcription factor TF2B]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 6 10712776
2024 An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of phenobarbital in human serum and plasma. Clinical chemistry and laboratory medicine 5 38407268
2022 Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice. Oxidative medicine and cellular longevity 5 36092156
2018 Rpb5, a subunit shared by eukaryotic RNA polymerases, cooperates with prefoldin-like Bud27/URI. AIMS genetics 5 31435513
1997 RMP-7 : Potential as an Adjuvant to the Drug Treatment of Brain Tumours. CNS drugs 5 27520751
1997 Intravenous infusion of RMP-7 increases ocular uptake of ganciclovir. Pharmaceutical research 5 9034225
2018 URI knockdown induces autophagic flux in gastric cancer cells. American journal of cancer research 4 30416863
1996 A competitive chemiluminescent enzyme-linked immunosorbent assay for the determination of RMP-7 in human blood. Journal of pharmaceutical and biomedical analysis 3 8887712
2016 P(URI)fying Novel Drivers of NASH and HCC: A Feedforward Loop of IL17A via White Adipose Tissue. Cancer cell 2 27411585
2025 Evaluation of the role of unconventional prefoldin RPB5 interactor (URI1) in hepatitis B virus infection. Virology journal 1 39794779
2014 [Biological function and molecular mechanism of URI in HepG2 cells]. Zhonghua zhong liu za zhi [Chinese journal of oncology] 1 25620477
2003 [Effect of RMP-7 and its derivatives on the transportation of liposome into the brain]. Yao xue xue bao = Acta pharmaceutica Sinica 1 14992004
2026 Hot-yet-suppressed under PD-1 blockade: an RMP-NRF2-PD-L1 axis associated with a reduced proportional response in hepatocellular carcinoma. Frontiers in immunology 0 41727467
2025 Retraction Notice to: The Expression of MicroRNA-598 Inhibits Ovarian Cancer Cell Proliferation and Metastasis by Targeting URI. Molecular therapy. Oncology 0 40487485
2019 RMP promotes the proliferation and radioresistance of esophageal carcinoma. Journal of Cancer 0 31333787
1996 Open-label titration study of the safety of RMP-7 in patients with the acquired immune deficiency syndrome. International journal of antimicrobial agents 0 18611707

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