Affinage

POLR2E

DNA-directed RNA polymerases I, II, and III subunit RPABC1 · UniProt P19388

Round 2 corrected
Length
210 aa
Mass
24.6 kDa
Annotated
2026-04-28
66 papers in source corpus 17 papers cited in narrative 17 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

POLR2E (RPB5/RPABC1) is a shared subunit of all three eukaryotic nuclear RNA polymerases that occupies a structurally critical position in the downstream DNA-gripping jaw of Pol II and functions in transcriptional activation, the initiation-to-elongation transition, and cytoplasmic polymerase assembly. Its bipartite architecture—comprising a eukaryote-specific N-terminal domain and an archaeal-homologous C-terminal domain—presents overlapping surface-exposed residues (F76, I104, T111, S113) that mediate direct contacts with the general transcription factor TFIIF (RAP30) and the viral transactivator HBx, while its C-terminal domain anchors it to the Rpb1 Bridge helix and Switch 1 loop within the polymerase cleft (PMID:10784442, PMID:16169872, PMID:17179178). A separation-of-function mutation (rpb5-P151T) demonstrated that RPB5 governs the initiation-to-elongation transition specifically in Pol II by promoting Spt5 recruitment and suppressing early backtracking, without affecting Pol I or Pol III (PMID:29133017). The prefoldin-like factor Bud27/URI uses an RPB5-binding domain to chaperone cytoplasmic assembly and nuclear import of all three RNA polymerases, and in the nucleus URI acts as a corepressor—for example of androgen receptor transcription—while also competing with NRF2 for KEAP1 binding to modulate oxidative stress signaling (PMID:23459708, PMID:21730289, PMID:31541481).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1995 High

    Establishing that RPB5 is a direct protein–protein interaction target of the HBx transactivator resolved how a viral oncoprotein could co-opt the general transcription machinery, and showed that the central region of RPB5 itself can stimulate transcription.

    Evidence In vitro binding with deletion mutants, co-IP from HepG2 cells, and CAT reporter assays

    PMID:7828586

    Open questions at the time
    • Whether HBx–RPB5 interaction occurs in the context of intact polymerase in vivo
    • Structural basis of HBx–RPB5 contact at atomic resolution
  2. 1996 High

    Mapping RPB5's direct contacts with Rpb1, Rpb2, and Rpb3 established the subunit-interaction network through which RPB5 is integrated into the polymerase, prior to any high-resolution structure.

    Evidence Far-Western and in vitro pulldown reconstitution in fission yeast

    PMID:9077438

    Open questions at the time
    • Whether RPB5–Rpb3 contact is functionally significant or merely structural
    • Contacts within Pol I and Pol III not addressed
  3. 1998 High

    Demonstration that an rpb5 mutation impairs GAL4-VP16 activated transcription in vitro and in vivo—and is synthetically lethal with CTD truncation—established RPB5 as a direct participant in transcriptional activation, not merely a structural scaffold.

    Evidence In vitro transcription with mutant extracts, in vivo reporter assays, and synthetic lethality in yeast

    PMID:9860960

    Open questions at the time
    • Which activators require RPB5 and which do not
    • Mechanism by which RPB5 and CTD cooperate
  4. 2000 High

    Crystal and NMR structures of RPB5 and of the complete 10-subunit Pol II revealed RPB5's bipartite fold and its position in the downstream DNA-gripping jaw alongside Rpb1 and Rpb9, providing an atomic framework for all subsequent interaction mapping.

    Evidence X-ray crystallography of yeast RPB5 and intact Pol II; NMR of archaeal RPB5

    PMID:10784442 PMID:10841537 PMID:10841538

    Open questions at the time
    • Dynamics of jaw opening/closing during transcription
    • How the eukaryote-specific N-terminal domain contributes to function beyond structure
  5. 2003 High

    Identification of RMP/URI as a corepressor acting through TFIIF and TFIIB via its D5 domain showed that RPB5-associated factors form a regulatory network linking the polymerase subunit to general factor-mediated repression.

    Evidence In vitro pulldown, Far-Western, co-IP from COS1 cells, and luciferase reporter assays

    PMID:12737519

    Open questions at the time
    • Whether RMP competes with or cooperates with RPB5 for TFIIF binding
    • Genome-wide target genes repressed through this mechanism
  6. 2005 High

    Systematic alanine-scanning mutagenesis pinpointed the precise residues on RPB5 that mediate overlapping yet distinguishable contacts with RAP30 and HBx, resolving which surface patches are shared versus unique.

    Evidence Two-step alanine scanning with binding assays in mammalian cells and in vitro

    PMID:16169872

    Open questions at the time
    • Whether these residues are also critical for URI/RMP binding
    • Structural validation of mutant effects by crystallography
  7. 2006 Medium

    Conditional rpb5 mutations in the C-terminal domain that alter Rpb1 Bridge-helix and Switch-1 contacts, combined with synthetic lethality with rpb9Δ, demonstrated functional cooperation between RPB5 and RPB9 in maintaining the transcription bubble cleft.

    Evidence Genetic analysis of conditional/lethal rpb5 mutants; two-hybrid; synthetic lethality with rpb9Δ in yeast

    PMID:17179178

    Open questions at the time
    • Biochemical basis of cleft destabilization in rpb5 mutants
    • Whether these mutations affect elongation or initiation
  8. 2011 High

    URI was shown to function as a hormone-regulated transcriptional corepressor of androgen receptor target genes, with phosphorylation-dependent chromatin occupancy, extending the RPB5-associated regulatory network to nuclear receptor signaling.

    Evidence siRNA, overexpression, genome-wide expression profiling, ChIP, and phosphorylation analysis in prostate cancer cells

    PMID:21730289

    Open questions at the time
    • Whether URI represses AR transcription directly through RPB5 or independently
    • Kinase responsible for URI phosphorylation upon androgen treatment
  9. 2013 High

    The discovery that the prefoldin-like factor Bud27/URI mediates cytoplasmic assembly and nuclear import of all three RNA polymerases in an RPB5-dependent manner revealed RPB5 as the key scaffold for polymerase biogenesis, not only catalytic function.

    Evidence Genetic rescue by RPB5 overexpression; cytoplasmic polymerase accumulation in bud27Δ; domain mapping in yeast

    PMID:23459708

    Open questions at the time
    • Order of subunit incorporation during assembly
    • Whether human URI performs the identical assembly role
  10. 2017 High

    A separation-of-function rpb5-P151T mutation that specifically impairs Pol II elongation—increasing backtracking, reducing Spt5 recruitment, and altering CTD phosphorylation—without affecting Pol I or Pol III established a Pol II-specific role for RPB5 in the initiation-to-elongation transition.

    Evidence Pol II ChIP, run-on analysis, genetic interaction with dst1Δ, Spt5 ChIP, and CTD phosphorylation state analysis in yeast

    PMID:29133017

    Open questions at the time
    • Structural mechanism by which P151T increases backtracking
    • Whether RPB5 directly contacts Spt5 or acts indirectly
  11. 2020 High

    RMP/URI was found to compete with NRF2 for KEAP1 binding via an E**E motif, stabilizing NRF2 and activating antioxidant gene expression—connecting the RPB5-associated corepressor network to oxidative stress and drug resistance in cholangiocarcinoma.

    Evidence Binding competition assays, ubiquitination assays, nuclear translocation imaging, and in vivo mouse tumor model

    PMID:31541481

    Open questions at the time
    • Whether RPB5 binding to URI is required for the KEAP1 competition
    • Relevance of this axis in non-cancer contexts

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RPB5 coordinates its roles in polymerase jaw dynamics, elongation factor docking, and URI-mediated assembly/repression at a single structural interface, and whether these represent mutually exclusive or sequential functions, remains unresolved.
  • No time-resolved structural data for RPB5 conformational changes during the transcription cycle
  • Whether IWS1 and URI bind RPB5 competitively or in distinct complexes
  • Full atomic-resolution structure of human RPB5 in complex with URI

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0140110 transcription regulator activity 3
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 2
Pathway
R-HSA-74160 Gene expression (Transcription) 4 R-HSA-8953854 Metabolism of RNA 2
Partners
DMAP1HBXRAP30RPB1RPB2RPB3RPB9URI1
Complex memberships
RNA polymerase IRNA polymerase IIRNA polymerase III

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Human RPB5 (POLR2E) directly binds the HBx transactivation domain; the central region of RPB5 is necessary for this interaction. The HBx-binding region of RPB5 alone can stimulate transcription from X-responsive element reporters, supporting a role for RPB5 in HBx-mediated transactivation. In vitro binding assays with deletion mutants of fusion proteins; co-immunoprecipitation from transfected HepG2 cells; CAT reporter assays The EMBO journal High 7828586
1998 A mutation in RPB5 (rpb5-9) impairs transcriptional activation by GAL4-VP16 both in vitro and in vivo, demonstrating a direct role for RPB5 in transcriptional activation at a subset of promoters. RPB5 and the RPB1-CTD have overlapping roles in activation, as the double mutant is synthetically lethal with exacerbated activation defects. The conserved human RPB5 region can functionally substitute for its yeast counterpart. In vitro transcription with mutant whole-cell extracts; in vivo reporter assays; Northern analysis; synthetic lethality analysis of double mutants Proceedings of the National Academy of Sciences of the United States of America High 9860960
1996 RPB5 (Rpb5) from fission yeast binds strongly to Rpb1 and Rpb2 (the two large subunits) and also contacts Rpb3, establishing a direct subunit-contact network within RNA polymerase II. Binary Rpb5–Rpb3 complexes form in vitro. Far-Western blot analysis with 32P-labeled GST-Rpb5 probe; in vitro complex formation with protein-immobilized beads Genes to cells : devoted to molecular & cellular mechanisms High 9077438
1998 Two-hybrid mapping showed that Rpb5 interacts with a region H-containing fragment of Rpb1 that is conserved across all RNA polymerases, including RNA pol I subunit Rpa190, consistent with Rpb5 being shared by all three nuclear polymerases. Yeast two-hybrid screening with Rpb1 and Rpb2 fragments from S. pombe in S. cerevisiae Molecular & general genetics : MGG Medium 9738888
2000 Crystal structure of yeast RPB5 reveals a bipartite architecture: a eukaryote-specific N-terminal domain and a C-terminal domain homologous to archaeal subunit H. Surface-exposed alpha-helical structures correspond to the experimentally mapped binding regions for TFIIB and HBx, indicating distinct interaction surfaces. X-ray crystallography Proceedings of the National Academy of Sciences of the United States of America High 10841537
2000 Solution NMR structure of archaeal RPB5 (from M. thermoautotrophicum) reveals a four-stranded beta-sheet supporting two alpha-helices in a mushroom shape with no structural homologues, providing a structural basis for understanding eukaryotic RPB5 and its protein–protein interaction surfaces. NMR solution structure determination Proceedings of the National Academy of Sciences of the United States of America High 10841538
2000 A backbone model of 10-subunit yeast RNA polymerase II at 3 Å shows that Rpb5 forms part of the 'jaws' structure together with Rpb1 and Rpb9 that grip downstream DNA, positioning RPB5 near the downstream DNA entry channel. X-ray crystallography of intact RNA polymerase II complex Science (New York, N.Y.) High 10784442
2003 RMP (URI/RPB5-mediating protein) suppresses activated transcription through interaction with TFIIF subunits RAP30 and RAP74; both subunits interact with the same C-terminal D5 domain of RMP that also binds TFIIB. Internal deletion of D5 abolishes both TFIIF binding and transcriptional repression, establishing that RMP modulates RPB5-associated transcription via TFIIF. In vitro pull-down assay; Far-Western analysis; co-immunoprecipitation from co-transfected COS1 cells; luciferase reporter assay Cell research High 12737519
2004 RMP (URI) localizes predominantly to the cytoplasm via a coiled-coil cytoplasmic localization signal (CLS), with a nuclear localization signal (NLS) that only becomes dominant when the CLS is absent. DMAP1 binds the CLS/CC domain of RMP and facilitates both RMP nuclear localization and its corepressor activity in a dose-dependent manner. GFP-fusion subcellular localization imaging; yeast two-hybrid; functional corepressor assay Molecular and cellular biology Medium 15367675
2005 Systematic alanine-scanning mutagenesis of human RPB5 identified residues F76, I104, T111, and S113 as critical for both TFIIF (RAP30) binding and HBx binding, revealing overlapping interaction surfaces. Additional residues V74 and N98 are required only for HBx-binding, while T56 and L58 are needed only for RAP30-binding. Two-step alanine scanning mutagenesis; binding assays in mammalian cells and in vitro Journal of biochemistry High 16169872
2006 Conditional and lethal mutations in yeast Rpb5 mapped to the N-terminal eukaryotic module and C-terminal globular domain; C-terminal mutations alter Rpb5 binding to Rpb1 regions that include the Bridge helix and Switch 1 loop. Synthetic lethality between rpb5 mutants and rpb9Δ supports a functional cooperation in maintaining the DNA-binding cleft around the transcription bubble. Genetic analysis of conditional/lethal mutants; two-hybrid assay; synthetic lethality analysis Nucleic acids research Medium 17179178
2011 URI (RMP/RPB5-mediating protein) is a transcriptional repressor of androgen receptor (AR)-mediated transcription: URI depletion enhances AR target gene expression while URI overexpression suppresses it. URI is phosphorylated upon androgen treatment, is bound to chromatin prior to hormone-dependent AR recruitment, and represses AR transcription in part by binding Art-27 (an AR corepressor). siRNA depletion; overexpression; genome-wide expression profiling; ChIP; phosphorylation analysis Molecular and cellular biology High 21730289
2013 The prefoldin Bud27 (yeast ortholog of human URI) mediates assembly of all three eukaryotic RNA polymerases in an Rpb5-dependent manner: loss of BUD27 causes cytoplasmic accumulation of RNA polymerases and defective incorporation of Rpb5 and Rpb6, and these defects are rescued by RPB5 overexpression. The Rpb5-binding domain (not the PFD-binding domain) of Bud27 is required for this function. Genetic analysis; overexpression rescue; localization studies; interaction domain mapping; growth assays PLoS genetics High 23459708
2013 URI nuclear interaction with RPB5 regulates RPB5 protein stability; URI also binds all components of the R2TP/prefoldin-like complex in the nucleus. URI nuclear/cytoplasmic shuttling is affected by compounds that stall Pol II (α-amanitin, actinomycin-D) and by the CRM1 inhibitor leptomycin B, suggesting URI (and the R2TP/prefoldin-like complex) is exported from the nucleus through CRM1. Mass spectrometry-based proteomic analysis; co-immunoprecipitation; pharmacological inhibition; subcellular fractionation PloS one Medium 23667685
2017 An rpb5-P151T mutation in yeast specifically impairs RNA polymerase II transcription elongation without affecting RNA pol I or pol III. The mutant shows increased backtracking tendency early after initiation, lower elongation rate, reduced Spt5 recruitment to chromatin, and altered Pol II CTD phosphorylation state, revealing that Rpb5 is required for the initiation-to-elongation transition. RNA pol II ChIP; run-on analysis; genetic interaction with dst1Δ; phosphorylation state analysis; Spt5 recruitment assay Biochimica et biophysica acta. Gene regulatory mechanisms High 29133017
2020 RMP (URI/RPB5-mediating protein) competes with NRF2 for binding to the Kelch domain of KEAP1 via an E**E motif, leading to decreased NRF2 ubiquitination and degradation, increased NRF2 nuclear translocation, and downstream antioxidant gene transactivation. This RMP–KEAP1–NRF2 axis promotes cholangiocarcinoma tumorigenesis and drug resistance. In vivo mouse cholangiocarcinoma model; mechanistic binding competition assays; ubiquitination assays; nuclear translocation imaging Hepatology (Baltimore, Md.) High 31541481
2025 Cryo-EM mapping of IWS1 short linear motifs (SLiMs) reveals that IWS1 contacts RPB5 (among other Pol II subunits RPB1 and RPB2) and elongation factors within the transcription elongation complex, establishing RPB5 as a structural docking site for the elongation factor IWS1. Cryo-electron microscopy; functional SLiM mapping; transcription assays bioRxivpreprint Medium bio_10.1101_2025.08.28.672863

Source papers

Stage 0 corpus · 66 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
1998 A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell 1086 9491887
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2005 Nucleolar proteome dynamics. Nature 934 15635413
1997 Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase. RNA (New York, N.Y.) 869 9409616
2020 A reference map of the human binary protein interactome. Nature 849 32296183
1996 The general transcription factors of RNA polymerase II. Genes & development 849 8946909
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2002 Comprehensive proteomic analysis of the human spliceosome. Nature 725 12226669
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2010 An atlas of combinatorial transcriptional regulation in mouse and man. Cell 573 20211142
2000 Architecture of RNA polymerase II and implications for the transcription mechanism. Science (New York, N.Y.) 452 10784442
2005 Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II. Cell 443 16239144
2011 HIV latency. Cold Spring Harbor perspectives in medicine 439 22229121
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2018 DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell 379 29656893
2007 Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Molecular cell 367 17643375
1999 HIV-1 tat transcriptional activity is regulated by acetylation. The EMBO journal 361 10545121
1995 Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation. The EMBO journal 230 7828586
2013 The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner. PLoS genetics 61 23459708
2013 Analysis of URI nuclear interaction with RPB5 and components of the R2TP/prefoldin-like complex. PloS one 54 23667685
2015 Long noncoding RNAs POLR2E rs3787016 C/T and HULC rs7763881 A/C polymorphisms are associated with decreased risk of esophageal cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 52 25874495
1998 RNA polymerase subunit RPB5 plays a role in transcriptional activation. Proceedings of the National Academy of Sciences of the United States of America 45 9860960
2011 Regulation of androgen receptor-mediated transcription by RPB5 binding protein URI/RMP. Molecular and cellular biology 33 21730289
2011 RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. The Journal of biological chemistry 32 21310960
2000 Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target. Proceedings of the National Academy of Sciences of the United States of America 32 10841537
2017 The HOTAIR, PRNCR1 and POLR2E polymorphisms are associated with cancer risk: a meta-analysis. Oncotarget 31 28159929
2003 Interaction with general transcription factor IIF (TFIIF) is required for the suppression of activated transcription by RPB5-mediating protein (RMP). Cell research 29 12737519
2006 Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases. Nucleic acids research 26 17179178
2004 Subcellular localization of RPB5-mediating protein and its putative functional partner. Molecular and cellular biology 25 15367675
1996 Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5. Genes to cells : devoted to molecular & cellular mechanisms 25 9077438
1999 Arabidopsis thaliana RNA polymerase II subunits related to yeast and human RPB5. Gene 21 10231567
2005 Mutational analysis of human RNA polymerase II subunit 5 (RPB5): the residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein. Journal of biochemistry 20 16169872
2020 RPB5-Mediating Protein Promotes Cholangiocarcinoma Tumorigenesis and Drug Resistance by Competing With NRF2 for KEAP1 Binding. Hepatology (Baltimore, Md.) 18 31541481
2000 Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum. Proceedings of the National Academy of Sciences of the United States of America 17 10841538
1998 Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast. Molecular & general genetics : MGG 16 9738888
1994 Evolutionary conservation and genomic organization of XAP-4, an Xq28 located gene coding for a human rab GDP-dissociation inhibitor (GDI). Mammalian genome : official journal of the International Mammalian Genome Society 14 7849400
2018 The association of POLR2E rs3787016 polymorphism and cancer risk: a Chinese case-control study and meta-analysis. Bioscience reports 8 30291213
1995 Sequence and functional analysis of a 7.2 kb DNA fragment containing four open reading frames located between RPB5 and CDC28 on the right arm of chromosome II. Yeast (Chichester, England) 8 7483850
2017 Rpb5 modulates the RNA polymerase II transition from initiation to elongation by influencing Spt5 association and backtracking. Biochimica et biophysica acta. Gene regulatory mechanisms 7 29133017
2015 RPB5-Mediating Protein Suppresses Hepatitis B Virus (HBV) Transcription and Replication by Counteracting the Transcriptional Activation of Hepatitis B virus X Protein in HBV Replication Mouse Model. Jundishapur journal of microbiology 7 26495109
2018 Long non-coding RNA POLR2E rs3787016 is associated with the risk of papillary thyroid carcinoma in Chinese population. Pathology, research and practice 6 29724531
2018 Long non-coding RNA POLR2E gene polymorphisms increased the risk of prostate cancer in a sample of the Iranian population. Nucleosides, nucleotides & nucleic acids 6 30587086
2000 [Study of HBV X protein and RMP, an RPB5 mediate protein competitively interacting with general transcription factor TF2B]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 6 10712776
1999 Crystallization and preliminary diffraction studies of the RNA polymerase subunit RPB5 from Saccharomyces cerevisiae. Acta crystallographica. Section D, Biological crystallography 6 10393311
2018 Rpb5, a subunit shared by eukaryotic RNA polymerases, cooperates with prefoldin-like Bud27/URI. AIMS genetics 5 31435513
2022 The unconventional prefoldin RPB5 interactor mediates the gravitropic response by modulating cytoskeleton organization and auxin transport in Arabidopsis. Journal of integrative plant biology 4 35943836
2015 Cloning, soluble expression, and purification of the RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. Bioengineered 4 25551420
2014 Activation of a chimeric Rpb5/RpoH subunit using library selection. PloS one 4 24489922
2021 RPB5-mediating protein promotes the progression of non-small cell lung cancer by regulating the proliferation and invasion. Journal of thoracic disease 3 33569210
2024 Associations of long non-coding RNAs HOTAIR, LINC00951, POLR2E and HULC polymorphisms with the risk of esophageal and esophagogastric junction cancer in a western population: a case-control study. Molecular biology reports 2 38300349
2025 Evaluation of the role of unconventional prefoldin RPB5 interactor (URI1) in hepatitis B virus infection. Virology journal 1 39794779
2011 Crystallization and preliminary X-ray analysis of the RPB5 subunit of human RNA polymerase II. Acta crystallographica. Section F, Structural biology and crystallization communications 1 22102239
2025 Protocatechuic Acid Alleviates Inflammation and Oxidative Stress in Acute Respiratory Distress Syndrome by Promoting Unconventional Prefoldin RPB5 Interactor 1-Mediated Mitophagy. Chemical biology & drug design 0 40040620