Affinage

SRCIN1

SRC kinase signaling inhibitor 1 · UniProt Q9C0H9

Length
1183 aa
Mass
127.1 kDa
Annotated
2026-06-10
73 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SRCIN1 (p140Cap/SNIP) is a multidomain adaptor/scaffold protein that integrates Src-family kinase signaling with actin cytoskeletal remodeling to control cell adhesion, motility, tumor progression, and synaptic organization (PMID:17525734, PMID:24453341). Its core biochemical function is negative regulation of Src: p140Cap recruits and activates C-terminal Src kinase (Csk), and this depends on Abl-mediated tyrosine phosphorylation of its EPLYA/EGLYA motifs, mutation of which abolishes Csk binding (PMID:17525734, PMID:23383002). Two independent regions—a tyrosine-enriched region carrying the EPLYA/EGLYA tyrosines and a C-terminal proline-rich region—each suffice to bind Csk and Src, dampen Src activation and FAK phosphorylation, and impair tumor cell growth and migration (PMID:23841028). In cancer, p140Cap acts as a broad suppressor of progression by immobilizing E-cadherin and inhibiting EGFR/Ras/Erk1/2 signaling (PMID:20453886), by binding cortactin to block invasion and metastasis (PMID:21725361), by inhibiting Tiam1-driven Rac GTPase circuitry (PMID:33415001, PMID:28300085), by stabilizing the β-catenin destruction complex to limit tumor-initiating-cell self-renewal and an immunosuppressive microenvironment (PMID:37169737), and by upregulating HMGCR to remodel membrane cholesterol and reduce raft-associated Rac1 signaling (PMID:38123597). In neurons, p140Cap is a postsynaptic and presynaptic hub that, through binding to SNAP-25, GluN2A, β-catenin, cortactin, Citron-N, endophilin A1, and SNX17, governs dendritic spine maturation, actin organization, synaptic plasticity, and GABAergic synaptogenesis, with knockout mice showing impaired LTP/LTD, abnormal spine morphology, altered inhibitory circuits, and seizure susceptibility (PMID:23868368, PMID:24453341, PMID:35953295, PMID:29161354). Loss of p140Cap in female mice also causes hypofertility via reduced glutamatergic input onto hypothalamic GnRH neurons (PMID:35237119).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2000 High

    Establishing the first molecular partner defined SRCIN1 as a SNAP-25-binding linker to the cortical cytoskeleton with a functional role in regulated exocytosis.

    Evidence Co-IP, deletion mapping, and exocytosis assays in PC12 cells

    PMID:10625663

    Open questions at the time
    • Did not identify catalytic activity or kinase-regulatory function
    • Coiled-coil binding mode not structurally resolved
  2. 2003 High

    Identification as a p130Cas-associated, adhesion- and EGF-induced tyrosine-phosphorylated protein linked SRCIN1 to integrin/EGFR signaling and cortical actin.

    Evidence Affinity chromatography/MS, reciprocal co-IP, cell spreading assays in NIH3T3/ECV304

    PMID:14657239

    Open questions at the time
    • Kinase responsible for phosphorylation not yet identified
    • Functional consequence limited to spreading delay
  3. 2007 High

    Defining p140Cap as a Csk-activating, Src-inhibiting protein established its central mechanism and its tumor-suppressive consequences.

    Evidence RNAi/overexpression, Src kinase assays, migration/invasion and in vivo breast tumor growth assays

    PMID:17525734

    Open questions at the time
    • Did not define how p140Cap engages Csk at the residue level
    • Csk recruitment mechanism unresolved
  4. 2010 High

    Showing p140Cap immobilizes E-cadherin and suppresses EGFR/Ras/Erk1/2 separated its adhesion-stabilizing and Ras-modulating roles from pure Src inhibition.

    Evidence Gain/loss-of-function, Ras activity assays, Src-rescue epistasis in breast/colon cancer cells

    PMID:20453886

    Open questions at the time
    • Direct mechanism of Ras inhibition not defined
    • Link between E-cadherin immobilization and Src inhibition mechanistically incomplete
  5. 2011 High

    Mapping the cortactin interaction and demonstrating phosphomimetic rescue established a causal actin/invasion axis for metastasis suppression.

    Evidence Orthotopic metastasis model, domain mapping, cortactin Y421 phosphomimetic rescue

    PMID:21725361

    Open questions at the time
    • Whether cortactin regulation is fully Src-dependent not isolated
    • In vivo cell-autonomy of effect not fully dissected
  6. 2013 High

    Identifying Abl-mediated EPLYA/EGLYA tyrosine phosphorylation as required for Csk binding, and mapping two independent inhibitory domains, defined the structural logic of Src regulation.

    Evidence In vivo MS phosphosite mapping, site-directed mutagenesis, in vitro kinase assays, domain-fragment cell lines with tumor assays

    PMID:23383002 PMID:23841028

    Open questions at the time
    • Stoichiometry and order of Abl/Csk/Src assembly unresolved
    • Role of the three serine phosphosites not characterized
  7. 2013 High

    Establishing SNAP-25-dependent recruitment of p140Cap to spines placed it as a postsynaptic regulator of actin and spine maturation.

    Evidence shRNA/overexpression, spine morphology, co-IP and live imaging in neurons

    PMID:23868368

    Open questions at the time
    • Downstream actin effectors at spines not fully mapped here
    • Synaptic plasticity readouts not yet tested
  8. 2014 High

    p140Cap knockout mice connected the protein to learning, LTP/LTD, and spine actin via Src inhibition and Citron-N binding, establishing physiological neuronal function.

    Evidence KO mouse behavior, LTP/LTD electrophysiology, spine/F-actin analysis, RhoA/cofilin assays, Citron-N co-IP

    PMID:24453341

    Open questions at the time
    • Relative contributions of Src vs Citron-N pathways not quantified
    • Cell-type specificity of behavioral deficit not resolved
  9. 2015 High

    Defining endophilin A1 as an upstream regulator that positions p140Cap and cortactin extended the spine actin regulatory pathway.

    Evidence Co-IP, reciprocal shRNA knockdown, spine morphology and electrophysiology

    PMID:25771685

    Open questions at the time
    • Direct vs indirect nature of endophilin A1 binding not fully established
    • Molecular trigger for the interaction unknown
  10. 2017 High

    Multiple studies expanded p140Cap's roles: presynaptic β-catenin partnership in spinogenesis, ERBB2/Rac-dependent tumor suppression, and a 351-protein synaptic interactome converging on transmission and actin.

    Evidence Conditional viral β-catenin expression with co-IP; NeuT mouse model with Rac assays; synaptosome co-IP/MS proteomics

    PMID:28300085 PMID:28641114 PMID:28713243

    Open questions at the time
    • Most interactome partners individually unvalidated
    • Mechanism linking β-catenin binding to presynaptic stabilization not defined
  11. 2019 High

    Cell-type-specific studies established a presynaptic GABAergic role controlling inhibitory circuits and seizure susceptibility, and extended tumor suppression to neuroblastoma via Src/STAT3.

    Evidence Global and interneuron-specific conditional KO with patch-clamp/RRP analysis and seizure assays; neuroblastoma gain/loss-of-function with xenografts

    PMID:29161354 PMID:31285546

    Open questions at the time
    • Molecular target controlling RRP size at GABAergic terminals not identified
    • Mechanism of STAT3 regulation not defined
  12. 2020 High

    Mapping the N-terminal Tiam1 interaction and demonstrating GEF inhibition mechanistically linked p140Cap to Rac suppression and E-cadherin stabilization.

    Evidence Domain mapping, co-IP, Tiam1 GEF activity assay, E-cadherin localization

    PMID:33415001

    Open questions at the time
    • Structural basis of Tiam1 inhibition not resolved
    • Single-lab validation
  13. 2022 High

    Demonstrating direct GluN2A binding and activity-dependent recruitment of GluN2A/PSD95 to lipid rafts defined a specific postsynaptic receptor-organizing function, and a parallel study tied p140Cap to female fertility via hypothalamic glutamatergic input.

    Evidence Co-IP, KO+rescue, lipid raft fractionation, STED microscopy, electrophysiology; KO endocrine/neuroanatomical analysis with KP stimulation

    PMID:35237119 PMID:35953295

    Open questions at the time
    • Mechanism coupling p140Cap to raft partitioning unresolved
    • Direct vs circuit-level cause of GnRH neuron loss not separated
  14. 2023 High

    Three studies added β-catenin destruction-complex stabilization with immunomodulatory consequences, HMGCR/mevalonate-pathway control of membrane cholesterol, and an SNX17 interaction required for spine maturation.

    Evidence Co-IP with destruction complex and G-CSF/in vivo tumor models; HMGCR/cholesterol flux and Rac1 assays with statin rescue; GST pulldown and Snx17 KO mouse

    PMID:37169737 PMID:37704928 PMID:38123597

    Open questions at the time
    • How p140Cap physically stabilizes the destruction complex unresolved
    • Transcriptional mechanism of HMGCR upregulation not defined
    • SNX17 interaction directness needs reciprocal validation
  15. 2025 Medium

    An affinity-quantified BIN1 interaction (and an AD-associated variant that weakens it) plus a centrosomal ciliogenesis role broadened p140Cap's adaptor functions into neurodegeneration-relevant and ciliary contexts.

    Evidence SPR, alanine-scanning, co-IP/PLA for BIN1; BioID, loss-of-function and pharmacological epistasis (actin/Src inhibitors) for ciliogenesis (one preprint)

    PMID:40889683 PMID:bio_10.1101_2025.10.30.685566

    Open questions at the time
    • Functional consequence of BIN1 binding in disease not established
    • Ciliogenesis findings are preprint and not peer-reviewed
    • Centrosomal Src target at cilia not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • How p140Cap's many distinct interaction modules are coordinated—whether tumor-suppressive and synaptic functions reflect a shared Src/actin core or context-specific assemblies—remains unresolved.
  • No structural model integrating the multiple binding domains
  • Determinants of context-specific partner selection unknown
  • Quantitative hierarchy among Src, Rac/Tiam1, and β-catenin axes undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0098772 molecular function regulator activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005886 plasma membrane 3 GO:0005856 cytoskeleton 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-112316 Neuronal System 4 R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 3 R-HSA-1430728 Metabolism 1
Partners
BIN1CSKCTNNB1CTTNGRIN2ASNAP25SRCTIAM1
Complex memberships
postsynaptic densityβ-Catenin destruction complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 SNIP (p140Cap/SRCIN1) was identified as a novel SNAP-25-interacting protein. Biochemical and deletion analysis demonstrated that the SNIP-SNAP-25 association is mediated via coiled-coil interactions. SNIP co-localizes with SNAP-25 and the cortical actin cytoskeleton, suggesting it serves as a linker between SNAP-25 and the submembranous cytoskeleton. Overexpression of SNIP or its SNAP-25-interacting domain inhibits Ca2+-dependent exocytosis from PC12 cells. Co-immunoprecipitation, deletion analysis, subcellular fractionation, immunofluorescence, overexpression in PC12 cells with exocytosis assay The Journal of biological chemistry High 10625663
2003 p140Cap was identified as a novel p130Cas-associated protein by affinity chromatography of human ECV304 cell extracts on an MBP-p130Cas column followed by mass spectrometry. Endogenous and transfected p140Cap co-immunoprecipitate with p130Cas and associate through their carboxy-terminal region. p140Cap co-distributes with cortical actin and actin stress fibers but not focal adhesions. p140Cap is tyrosine-phosphorylated within 15 min of cell adhesion to integrin ligands and in response to EGF via an EGFR-dependent mechanism. Expression of p140Cap delays cell spreading in the early phases of adhesion to fibronectin. Affinity chromatography, mass spectrometry, co-immunoprecipitation, immunofluorescence, subcellular fractionation, overexpression in NIH3T3 and ECV304 cells Molecular biology of the cell High 14657239
2007 p140Cap was characterized as a novel Src-binding protein that regulates Src activation via C-terminal Src kinase (Csk). p140Cap silencing increases cell spreading, migration rate and Src kinase activity. Increased p140Cap expression activates Csk, leading to inhibition of Src and downstream signaling, as well as inhibition of cell motility and invasion. High levels of p140Cap strongly impair cell proliferation and in vivo breast cancer cell growth. RNA silencing, overexpression, Src kinase activity assay, cell migration/invasion assays, in vivo tumor growth assay, co-immunoprecipitation The EMBO journal High 17525734
2008 p140Cap is expressed in rat brain in a developmental stage-dependent manner and is relatively abundant in the synaptic plasma membrane fraction in adults. Electron microscopy revealed localization at pre- and post-synapses. The third SH3 domain of vinexin interacts with the C-terminal Pro-rich motif of p140Cap; this interaction was confirmed by co-immunoprecipitation in COS7 cells and rat brain. Additionally, the pre-synaptic protein synaptophysin interacts with p140Cap. Subcellular fractionation, immunohistochemistry, electron microscopy, co-immunoprecipitation, pulldown/binding domain mapping Journal of neurochemistry High 18662323
2010 p140Cap immobilizes E-cadherin at the cell membrane and inhibits EGFR and Erk1/2 signaling, blocking scatter and proliferation of cancer cells. p140Cap-dependent regulation of E-cadherin/EGFR cross-talk and cell motility is due to inhibition of Src kinase. Additionally, p140Cap impairs Erk1/2 phosphorylation by affecting Ras activity downstream of EGFR, independent of Src activity restoration. Gain and loss-of-function approaches in breast and colon cancer cells, Western blotting for phospho-EGFR/Erk1/2, Ras activity assay, cell motility assays, co-immunoprecipitation Oncogene High 20453886
2011 p140Cap suppresses invasive properties of highly metastatic breast carcinoma cells by inhibiting cortactin-dependent cell motility. p140Cap over-expression causes ~80% inhibition of lung metastasis in orthotopic transplantation. p140Cap associates with cortactin via its second proline-rich domain binding to the cortactin SH3 domain, and inhibits cortactin tyrosine phosphorylation in response to EGF. The phosphomimetic cortactin Y421 mutant rescues migration and invasion in p140Cap-overexpressing cells. Orthotopic transplantation in mice, directional migration assay, 3D invasion assay, co-immunoprecipitation, Western blotting, rescue with phosphomimetic cortactin mutant Oncogene High 21725361
2013 SNAP-25 regulates spine morphogenesis through postsynaptic binding to p140Cap. Acute reduction of SNAP-25 leads to immature dendritic spine phenotype; overexpression increases density of mature PSD-95-positive spines. The regulation depends on SNAP-25's ability to bind both the plasma membrane and p140Cap. SNAP-25 recruits and stabilizes p140Cap, which is a key regulator of actin cytoskeleton and spine formation. shRNA knockdown, overexpression, immunofluorescence, spine morphology analysis, co-immunoprecipitation, live imaging Nature communications High 23868368
2013 Mass spectrometry identified that p140Cap is in vivo phosphorylated on tyrosine within the EGLYA motif (GEGLpYADPYGLLHEGR) as well as on three serine residues. Site-specific mutagenesis of the EPLYA/EGLYA tyrosines to phenylalanine abolishes p140Cap tyrosine phosphorylation and eliminates its ability to bind Csk. Abl kinase was identified as the major kinase responsible for p140Cap tyrosine phosphorylation on EPLYA and EGLYA sequences both in vitro and in HEK-293 cells. Mass spectrometry, site-directed mutagenesis, Far Western analysis, in vitro kinase assay, co-immunoprecipitation in HEK-293 cells PloS one High 23383002
2013 Two domains of p140Cap, TER (Tyrosine Enriched Region containing EPLYA and EGLYA motifs) and CT (Carboxy Terminal with proline-rich sequence), each independently associate with Csk and Src, inhibit Src activation and FAK phosphorylation, and impair in vitro and in vivo tumor cell growth and directional migration. The ability to act as negative regulators mainly resides on the two tyrosines in EPLYA/EGLYA (TER) and the second proline-rich stretch (CT). Stable cell lines expressing domain fragments, kinase activity assays, point mutation/deletion mutagenesis, in vitro migration/proliferation assays, in vivo tumor assay American journal of cancer research High 23841028
2014 p140Cap knockout mice are impaired in object recognition and show deficits in LTP and LTD. Primary neurons from p140Cap-/- mice show reduced mushroom spines and abnormal F-actin organization. These phenotypes are caused by reduced inhibitory control of RhoA and cortactin toward cofilin. p140Cap acts through direct inhibition of Src kinase activation and through binding to the scaffold protein Citron-N to control spine actin organization. Knockout mouse model, behavioral tests (object recognition), LTP/LTD electrophysiology, spine morphology analysis, F-actin staining, co-immunoprecipitation with Citron-N, RhoA/cofilin activity assays The Journal of neuroscience High 24453341
2015 Endophilin A1 localizes to dendritic spines and regulates spine morphogenesis through p140Cap as a downstream effector. Disruption of the endophilin A1–p140Cap interaction impairs spine formation and maturation. Endophilin A1 regulates the distribution of p140Cap and its downstream effector cortactin, as well as F-actin enrichment in dendritic spines. Knockdown of either endophilin A1 or p140Cap impairs spine stabilization and synaptic function. Co-immunoprecipitation, shRNA knockdown, immunofluorescence, spine morphology analysis, electrophysiology Cell research High 25771685
2017 Presynaptic β-catenin expression upregulates excitatory synaptic transmission and dendritic spine density. p140Cap is a newly identified β-catenin-interacting protein required in the presynaptic locus for mediating these effects on synapse stabilization and spinogenesis in neocortex. Conditional viral expression of stabilized β-catenin in defined neuron layers, electrophysiology, spine density analysis, co-immunoprecipitation identifying p140Cap as β-catenin interactor Neuron High 28641114
2017 p140Cap dampens ERBB2-positive tumor cell progression by interfering with ERBB2-dependent activation of Rac GTPase-controlled circuitries. p140Cap impairs tumor onset and growth in the NeuT mouse model and counteracts EMT, resulting in decreased metastasis formation. NeuT transgenic mouse model, gain/loss of function in ERBB2-positive cell lines, Rac GTPase activity assays, EMT marker analysis, in vivo tumor growth and metastasis assays Nature communications High 28300085
2017 p140Cap synaptic interactome isolated by co-immunoprecipitation from mouse synaptosomes and mass spectrometry identified 351 p140Cap interactors clustering to postsynaptic density sub-complexes. Interactors converge on synaptic transmission, actin cytoskeleton remodeling, and cell-cell junction organization. Co-immunoprecipitation from crude mouse synaptosomes, mass spectrometry-based proteomics, gene co-expression analysis Frontiers in molecular neuroscience Medium 28713243
2019 p140Cap regulates GABAergic synaptogenesis and hippocampal inhibitory circuits. p140Cap is present in presynaptic GABAergic terminals; its genetic depletion results in higher frequency of mIPSCs without amplitude change, a larger synaptic vesicle readily releasable pool, increased number of inhibitory synapses, increased parvalbumin- and somatostatin-expressing interneurons, and enhanced susceptibility to kainate-induced seizures. Specific deletion of p140Cap in forebrain interneurons recapitulates these effects. p140Cap-/- knockout mice, cell-type-specific conditional knockout (forebrain interneurons), patch-clamp electrophysiology (mIPSC, RRP analysis), immunohistochemistry, in vitro/in vivo seizure assays Cerebral cortex High 29161354
2019 p140Cap negatively regulates Src and STAT3 signaling in neuroblastoma cells, affects anchorage-independent growth and migration, impairs in vivo tumor growth and spontaneous lung metastasis formation, and increases sensitivity to doxorubicin, etoposide, and Src inhibitors. Gain and loss of function experiments, Src and STAT3 activity assays, anchorage-independent growth assay, migration assay, xenograft mouse model Cell death and differentiation High 31285546
2020 The N-terminal domain of p140Cap (amino acids 1–287) is sufficient to associate with full-length Tiam1 and the truncated catalytic domain of Tiam1, causing a decrease in Tiam1 activity. p140Cap expression causes Tiam1 redistribution along the apicobasal junctional axis and p140Cap, Tiam1, and E-cadherin co-interact, with increased E-cadherin at the cell membrane. Biochemical domain mapping, co-immunoprecipitation, Tiam1 GEF activity assay, immunofluorescence, E-cadherin membrane localization analysis American journal of cancer research High 33415001
2022 p140Cap directly binds the GluN2A subunit of NMDAR. In p140Cap KO mice, GluN2A is less associated with PSD95 in ex vivo synaptosomes and cultured hippocampal neurons; p140Cap re-expression rescues GluN2A/PSD95 colocalization. p140Cap associates with synaptic lipid rafts and controls selective recruitment of GluN2A and PSD95 to lipid raft domains in an activity-dependent fashion. p140Cap is required for embedding GluN2A clusters in lipid rafts. Co-immunoprecipitation, KO mouse model, rescue experiments by transfection, subcellular fractionation (lipid raft isolation), gated-STED microscopy, electrophysiology The Journal of neuroscience High 35953295
2022 p140Cap loss-of-function in female mice leads to severe hypofertility, delayed puberty, altered estrus cycle, reduced ovulation, and defective LH/estradiol production. Adult p140Cap KO mice show loss of GnRH-immunoreactive neurons and reduced GnRH/LH mRNAs. The glutamatergic circuitry (VGLUT-ir punctae) in the preoptic area and on GnRH neurons is significantly reduced in p140Cap KO mice, indicating p140Cap controls female fertility via glutamatergic afference on hypothalamic GnRH neurons. p140Cap KO mouse model, immunohistochemistry for GnRH/kisspeptin/VGLUT, hormone measurements, in vivo KP stimulation assay Frontiers in neuroscience High 35237119
2023 p140Cap inhibits β-Catenin by localizing in and stabilizing the β-Catenin destruction complex, promoting enhanced β-Catenin inactivation. Through upstream inhibition of β-Catenin, p140Cap restricts tumorigenicity and self-renewal of tumor-initiating cells, limiting release of inflammatory cytokine G-CSF, which is required for polymorphonuclear myeloid-derived suppressor cells to create an immunosuppressive tumor-promoting environment. Transcriptomic analysis, co-immunoprecipitation with β-Catenin destruction complex components, loss-of-function/gain-of-function experiments, G-CSF measurement, in vivo tumor models Nature communications High 37169737
2023 p140Cap positively regulates HMGCR (the pace-maker enzyme of the mevalonate pathway) via transcriptional and post-translational mechanisms, increasing flux through the MVA pathway. Higher cholesterol synthesis is paralleled with enhanced cholesterol efflux. p140Cap promotes increased cholesterol localization in the plasma membrane, reduces lipid raft-associated Rac1 signaling, and impairs cell membrane fluidity and cell migration in a cholesterol-dependent manner. In vitro and in vivo p140Cap expression models, HMGCR activity/expression analysis, cholesterol flux assays, lipid raft fractionation, Rac1 activity assay, migration assays, statin sensitivity assays Cell death & disease Medium 38123597
2023 SNX17 interacts with p140Cap (identified by GST pulldown and interactome analysis). This interaction is crucial for dendritic spine maturation. Snx17 haploinsufficiency leads to impaired synaptic transmission and reduced spine maturation, phenocopying p140Cap loss. GST pulldown, interactome analysis, Snx17 knockout mice, spine morphology analysis, electrophysiology Molecular neurobiology Medium 37704928
2025 BIN1 SH3 domain interacts with p140Cap through two class II proline-rich motifs (but not a class I motif) in p140Cap, with biologically relevant affinity (KD = 7.7 μM). A rare BIN1 coding variant (rs138047593) significantly reduces p140Cap and tau binding. BIN1 and p140Cap colocalize within molecular distance in cultured cells and mouse brain. Surface plasmon resonance, alanine-scanning mutagenesis, co-immunoprecipitation, GST pulldown, confocal microscopy, proximity ligation assay The Journal of biological chemistry High 40889683
2025 p140Cap (and its paralog KIAA1217/SKT) localizes to centrosomes and regulates ciliogenesis through Src family signaling. Loss of p140Cap impairs ciliogenesis in RPE-1 cells. Defects caused by p140Cap loss are rescued by inhibiting actin polymerization or Src activity, establishing that p140Cap regulates actin polymerization through Src family kinase activity at centrosomes/cilia. BioID proximity labeling, loss-of-function (siRNA/KO), ciliogenesis assay (cilia number/length), rescue with actin polymerization inhibitors and Src inhibitors bioRxivpreprint Medium bio_10.1101_2025.10.30.685566

Source papers

Stage 0 corpus · 73 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 Breast cancer cell-derived exosomal miR-20a-5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1. Cancer medicine 97 31385464
2000 SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. The Journal of biological chemistry 84 10625663
2018 MicroRNA-181a promotes angiogenesis in colorectal cancer by targeting SRCIN1 to promote the SRC/VEGF signaling pathway. Cell death & disease 80 29739921
2007 p140Cap protein suppresses tumour cell properties, regulating Csk and Src kinase activity. The EMBO journal 75 17525734
2014 miR-374a promotes cell proliferation, migration and invasion by targeting SRCIN1 in gastric cancer. FEBS letters 69 25554419
2015 miR-873 induces lung adenocarcinoma cell proliferation and migration by targeting SRCIN1. American journal of translational research 66 26807196
2013 SNAP-25 regulates spine formation through postsynaptic binding to p140Cap. Nature communications 63 23868368
2003 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Molecular biology of the cell 56 14657239
2010 p140Cap dual regulation of E-cadherin/EGFR cross-talk and Ras signalling in tumour cell scatter and proliferation. Oncogene 50 20453886
2014 p140Cap regulates memory and synaptic plasticity through Src-mediated and citron-N-mediated actin reorganization. The Journal of neuroscience : the official journal of the Society for Neuroscience 47 24453341
2016 MiR-346 promotes the biological function of breast cancer cells by targeting SRCIN1 and reduces chemosensitivity to docetaxel. Gene 41 27913185
2017 A Critical Role of Presynaptic Cadherin/Catenin/p140Cap Complexes in Stabilizing Spines and Functional Synapses in the Neocortex. Neuron 40 28641114
2015 miR-211 promotes non-small cell lung cancer proliferation by targeting SRCIN1. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 40 26277787
2015 Endophilin A1 regulates dendritic spine morphogenesis and stability through interaction with p140Cap. Cell research 37 25771685
2022 How bacteria utilize sialic acid during interactions with the host: snip, snatch, dispatch, match and attach. Microbiology (Reading, England) 36 35316172
2018 MiR-150 promotes angiogensis and proliferation of endothelial progenitor cells in deep venous thrombosis by targeting SRCIN1. Microvascular research 36 30315850
2017 Genome-wide association studies of smooth pursuit and antisaccade eye movements in psychotic disorders: findings from the B-SNIP study. Translational psychiatry 36 29064472
2008 Characterization of a multidomain adaptor protein, p140Cap, as part of a pre-synaptic complex. Journal of neurochemistry 30 18662323
2023 Ginsenoside Rh2 suppresses colon cancer growth by targeting the miR-150-3p/SRCIN1/Wnt axis. Acta biochimica et biophysica Sinica 25 36916297
2019 miR-150-5p promotes the proliferation and epithelial-mesenchymal transition of cervical carcinoma cells via targeting SRCIN1. Pathology, research and practice 25 30679084
2018 Hepatitis B virus promotes proliferation and metastasis in male Chinese hepatocellular carcinoma patients through the LEF-1/miR-371a-5p/SRCIN1/pleiotrophin/Slug pathway. Experimental cell research 24 29928866
2017 Synaptic Interactome Mining Reveals p140Cap as a New Hub for PSD Proteins Involved in Psychiatric and Neurological Disorders. Frontiers in molecular neuroscience 23 28713243
2010 Survey of Neonates in Pomerania (SNiP): a population-based birth study--objectives, design and population coverage. Paediatric and perinatal epidemiology 23 20415776
2015 Genetic Sources of Subcomponents of Event-Related Potential in the Dimension of Psychosis Analyzed From the B-SNIP Study. The American journal of psychiatry 22 25615564
2019 MicroRNA-665 promotes the proliferation of ovarian cancer cells by targeting SRCIN1. Experimental and therapeutic medicine 19 32010277
2016 SRCIN1 Suppressed Osteosarcoma Cell Proliferation and Invasion. PloS one 19 27513473
2013 Identification of two regions in the p140Cap adaptor protein that retain the ability to suppress tumor cell properties. American journal of cancer research 19 23841028
2011 p140Cap suppresses the invasive properties of highly metastatic MTLn3-EGFR cells via impaired cortactin phosphorylation. Oncogene 19 21725361
2011 The adaptor proteins p140CAP and p130CAS as molecular hubs in cell migration and invasion of cancer cells. American journal of cancer research 19 21994904
2019 Role of microRNA-150-5p/SRCIN1 axis in the progression of breast cancer. Experimental and therapeutic medicine 18 30867707
2019 The SRCIN1/p140Cap adaptor protein negatively regulates the aggressiveness of neuroblastoma. Cell death and differentiation 17 31285546
2017 The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nature communications 17 28300085
2019 p140Cap Regulates GABAergic Synaptogenesis and Development of Hippocampal Inhibitory Circuits. Cerebral cortex (New York, N.Y. : 1991) 16 29161354
2018 Comparison of PCR Methods for Onchocerca volvulus Detection in Skin Snip Biopsies from the Tshopo Province, Democratic Republic of the Congo. The American journal of tropical medicine and hygiene 15 29611501
2019 MicroRNA-374a promotes pancreatic cancer cell proliferation and epithelial to mesenchymal transition by targeting SRCIN1. Pathology, research and practice 14 30890278
2017 Overexpression of Srcin1 contributes to the growth and metastasis of colorectal cancer. International journal of oncology 14 28393242
2013 Mapping of p140Cap phosphorylation sites: the EPLYA and EGLYA motifs have a key role in tyrosine phosphorylation and Csk binding, and are substrates of the Abl kinase. PloS one 14 23383002
2020 SRCIN1 Regulated by circCCDC66/miR-211 Is Upregulated and Promotes Cell Proliferation in Non-Small-Cell Lung Cancer. BioMed research international 13 32964035
2004 3'-End polishing of the kinetoplastid spliced leader RNA is performed by SNIP, a 3'-->5' exonuclease with a Motley assortment of small RNA substrates. Molecular and cellular biology 13 15542846
2023 p140Cap inhibits β-Catenin in the breast cancer stem cell compartment instructing a protective anti-tumor immune response. Nature communications 12 37169737
2023 p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells. Cell death & disease 12 38123597
2017 [ARTICLE WITHDRAWN] Downregulation of SRC Kinase Signaling Inhibitor 1 (SRCIN1) Expression by MicroRNA-32 Promotes Proliferation and Epithelial-Mesenchymal Transition in Human Liver Cancer Cells. Oncology research 12 28550679
2021 Real-time facial emotion recognition deficits across the psychosis spectrum: A B-SNIP Study. Schizophrenia research 11 34887147
2020 LincRNA00494 Suppresses Non-small Cell Lung Cancer Cell Proliferation by Regulating SRCIN1 Expression as a ceRNA. Frontiers in oncology 11 32117734
2018 VEGFA GENE variation influences hallucinations and frontotemporal morphology in psychotic disorders: a B-SNIP study. Translational psychiatry 11 30310054
2019 miR-373 promotes neuroblastoma cell proliferation, migration, and invasion by targeting SRCIN1. OncoTargets and therapy 9 31417287
2002 Comparison between the skin snip test and simple dot blot assay as potential rapid assessment tools for Onchocerciasis in the postcontrol era in Ghana. Clinical and diagnostic laboratory immunology 9 12204952
2023 Effect of genetic polymorphisms on outcomes following nivolumab for advanced renal cell carcinoma in the SNiP-RCC trial. Cancer immunology, immunotherapy : CII 8 36729213
2021 p130Cas/BCAR1 and p140Cap/SRCIN1 Adaptors: The Yin Yang in Breast Cancer? Frontiers in cell and developmental biology 8 34708040
2022 p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts. The Journal of neuroscience : the official journal of the Society for Neuroscience 7 35953295
2020 The p140Cap adaptor protein as a molecular hub to block cancer aggressiveness. Cellular and molecular life sciences : CMLS 7 33079227
2019 Dissecting the Shared and Context-Dependent Pathways Mediated by the p140Cap Adaptor Protein in Cancer and in Neurons. Frontiers in cell and developmental biology 7 31681758
1999 A novel pancreatic model: the snip method of pancreatic isolation for in vitro study. Pancreas 7 10547198
2020 The N-terminal domain of the adaptor protein p140Cap interacts with Tiam1 and controls Tiam1/Rac1 axis. American journal of cancer research 5 33415001
2013 Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats. Medical molecular morphology 5 23325552
2023 SNX17 Mediates Dendritic Spine Maturation via p140Cap. Molecular neurobiology 4 37704928
2022 miR-657 Targets SRCIN1 via the Slug Pathway to Promote NSCLC Tumor Growth and EMT Induction. Disease markers 4 36033827
2020 Correction to: The SRCIN1/p140Cap adaptor protein negatively regulates the aggressiveness of neuroblastoma. Cell death and differentiation 4 31488891
2016 Role of the Mdm2 SNIP 309 Polymorphism in Gastric Mucosal Morphologic Patterns of Patients with Helicobacter pylori Associated Gastritis. Asian Pacific journal of cancer prevention : APJCP 4 27039725
2024 Effect of HLA Genotype on Anti-PD-1 Antibody Treatment for Advanced Renal Cell Carcinoma in the SNiP-RCC Study. Journal of immunology (Baltimore, Md. : 1950) 3 38758119
2023 Clinical factors for tumor response, progression, and survival in nivolumab for advanced renal cell carcinoma in the SNiP-RCC study. International journal of urology : official journal of the Japanese Urological Association 3 37528632
2015 Correlation between Patterns of Mdm2 SNIP 309 and Histopathological Severity of Helicobacter pylori Associated Gastritis in Thailand. Asian Pacific journal of cancer prevention : APJCP 3 26625797
1997 [Endothelial cell loss after phacoemulsification with the "reversed tip and snip" technique compared with the "divide and conquer" technique]. Klinische Monatsblatter fur Augenheilkunde 3 9229600
2018 Author Correction: The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nature communications 2 29600801
2017 Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers. BioTechniques 2 28298176
2023 Hsa_circ_0046430 promotes the progression of colorectal cancer by targeting miR-6785-5p/SRCIN1 axis as a ceRNA. Medicine 1 36827049
2022 p140Cap Controls Female Fertility in Mice Acting via Glutamatergic Afference on Hypothalamic Gonadotropin-Releasing Hormone Neurons. Frontiers in neuroscience 1 35237119
1998 [Experiences with "reversed tip and snip" phacoemulsification]. Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft 1 9738377
2025 Predictive Model of Objective Response to Nivolumab Monotherapy for Advanced Renal Cell Carcinoma by Machine Learning Using Genetic and Clinical Data: The SNiP-RCC Study. JCO clinical cancer informatics 0 40279530
2025 "Snip, snip, cure"? Philosophical, legal and biomedical perspectives on novel somatic genomic therapies. Medicine, health care, and philosophy 0 40788566
2025 SRCIN1 promotes thyroid carcinoma growth by activating Wnt signaling pathway. Scientific reports 0 40849352
2025 A biophysical and molecular characterization of the interaction between the Alzheimer risk factor BIN1 and the neuronal scaffold protein p140Cap. The Journal of biological chemistry 0 40889683
2009 GEN-SNiP: an online tool to find polymorphisms in a genome. In silico biology 0 22430435

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