Affinage

SGO1

Shugoshin 1 · UniProt Q5FBB7

Length
561 aa
Mass
64.2 kDa
Annotated
2026-06-10
55 papers in source corpus 26 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SGO1 (Shugoshin 1) is a centromeric adaptor protein that protects centromeric cohesin during mitosis and meiosis and enforces accurate chromosome biorientation (PMID:15637284, PMID:16357219, PMID:23242214). Its core protective function is to recruit and position protein phosphatase 2A (PP2A): SGO1 binds PP2A directly, and this interaction is required for SGO1's own centromeric residence (PMID:16580887), while in budding yeast artificial tethering of the PP2A-Rts1 isoform is sufficient to substitute for Sgo1 on a chromosome (PMID:25236599). CDK-mediated mitosis-specific phosphorylation activates SGO1 to bind a cohesin complex containing PP2A, PDS5, and hypophosphorylated sororin but lacking WAPL, where SGO1-PP2A dephosphorylates PDS5-bound sororin to shield centromeric cohesin from WAPL-mediated removal (PMID:23242214). SGO1 is targeted to centromeres through layered cues: HP1-dependent recruitment to centromeric heterochromatin in G2 (PMID:20124418, PMID:21346195), a kinetochore pool established by reading the Bub1-deposited histone H2A-pT120 mark (PMID:24055156, PMID:27116032), and CENP-A binding via basic C-terminal residues that anchors SGO1 and Aurora B at the centromere (PMID:37777834, PMID:28980861). These distinct pools — a cohesin-bound inner-centromere pool and an H2A-pT120-bound kinetochore pool — are redistributed in response to kinetochore tension to couple cohesion protection to biorientation (PMID:24055156). SGO1 additionally scaffolds the chromosomal passenger complex, binding the Survivin BIR domain through its histone-H3-like N-terminal tail and the Borealin dimerization domain to drive CPC centromere localization and Aurora B function (PMID:35776132, PMID:32697622). Its activity is tuned by phosphorylation (NEK2A, Aurora B) (PMID:17621308, PMID:25451264) and antagonized by SET, which disrupts the SGO1-cohesin interaction to permit timely segregation (PMID:31227592), and SGO1 is degraded at mitotic exit by APC/C-Cdh1 acting on its KEN box and D-box (PMID:19015261). A homozygous SGOL1 K23E founder mutation causes CAID syndrome, with patient cells showing centromeric cohesion defects and the phenotype recapitulated by zebrafish knockdown (PMID:25282101).

Mechanistic history

Synthesis pass · year-by-year structured walk · 26 steps
  1. 2005 High

    Established that Sgo1 is required not for sensing microtubule occupancy but specifically for sensing tension at kinetochores and for sister-chromatid biorientation, defining its central role in chromosome segregation fidelity.

    Evidence Genetic mutant analysis with spindle checkpoint and segregation assays in S. cerevisiae

    PMID:15637284

    Open questions at the time
    • Molecular basis of how Sgo1 transduces tension was not defined
    • Did not identify the cohesin-protection biochemistry in human cells
  2. 2005 High

    Mapped the meiotic Sgo1-binding domain to a 50-kb pericentric region where cohesins are protected, and showed its establishment depends on the core centromere, Bub1, and Spo13.

    Evidence ChIP domain mapping and genetic epistasis in S. cerevisiae meiosis

    PMID:16357219

    Open questions at the time
    • Did not establish the protective enzymatic mechanism
    • Meiosis-specific factors may not generalize to mitosis or mammals
  3. 2006 High

    Identified PP2A as a direct SGO1 partner and showed the interaction is mutually required for centromeric localization, counteracting Plk1-mediated removal, establishing the SGO1-PP2A axis as the effector of cohesion protection.

    Evidence Reciprocal Co-IP, RNAi epistasis, and immunofluorescence in human cells

    PMID:16580887

    Open questions at the time
    • Did not show how PP2A protects cohesin biochemically
    • Substrate of the recruited phosphatase not yet identified
  4. 2006 Medium

    Distinguished two human SGO1 isoforms with separable localizations, foreshadowing isoform-specific functions at kinetochores versus spindles/centrosomes.

    Evidence GFP-fusion live-cell imaging and CREST co-localization in human cells

    PMID:16582621

    Open questions at the time
    • Functional consequences of isoform localization not tested here
    • Single-lab localization without functional perturbation
  5. 2007 High

    Showed NEK2A directly phosphorylates SGO1 at defined sites to support chromosome congression and correct attachment, adding a kinase regulatory input distinct from kinetochore assembly.

    Evidence In vitro kinase assay with site mapping and phospho-mutant imaging

    PMID:17621308

    Open questions at the time
    • How phosphorylation alters SGO1 partner binding not resolved
    • Phosphorylation dispensable for kinetochore assembly, leaving its precise role partial
  6. 2008 High

    Defined the short splice variant sSgo1 as a centrosome/spindle-pole protein that maintains centriole cohesion through a Plk1-regulated mechanism, separating a non-cohesin SGO1 function.

    Evidence GFP localization, reciprocal Co-IP, RNAi rescue, and Plk1 epistasis in human cells

    PMID:18331714

    Open questions at the time
    • Molecular mechanism of centriole cohesion by sSgo1 not detailed
    • Relationship between sSgo1 and full-length cohesin protection unclear
  7. 2008 High

    Identified SGO1 as an APC/C-Cdh1 substrate requiring both KEN box and D-box for degradation, but showed degradation is dispensable for mitotic progression, clarifying that turnover is regulatory rather than essential for segregation.

    Evidence In vitro ubiquitination, degron mutagenesis, and cell-cycle synchronization in human cells

    PMID:19015261

    Open questions at the time
    • Functional purpose of SGO1 degradation not established
    • Bub1-dependent steady-state control mechanism left undefined
  8. 2009 High

    Demonstrated biochemically that Sgo1 stabilizes arm cohesin in prophase and that Plk1 and Aurora B antagonize this to drive sister resolution, placing SGO1 within the prophase pathway.

    Evidence Xenopus egg extract reconstitution with immunodepletion and rescue

    PMID:19696148

    Open questions at the time
    • Direct substrate of the antagonizing kinases on SGO1 not mapped
    • Single cell-free system
  9. 2010 Medium

    Resolved the temporal recruitment logic by showing G2 centromeric loading is HP1-dependent and Bub1-kinase-independent, and that this early pool, not the mitotic Bub1-dependent pool, suffices to establish cohesion protection.

    Evidence BUB1-null/kinase-dead MEF complementation with staged immunofluorescence

    PMID:20124418

    Open questions at the time
    • Why mitotic centromeric SGO1 is dispensable for cohesion not mechanistically explained
    • Single lab
  10. 2011 High

    Separated SGO1's HP1- and CPC-dependent localization modes, showing HP1 binding governs interphase localization but is dispensable for mitotic cohesion protection.

    Evidence Structural/biochemical analysis, mutagenesis, and complementation in human cells

    PMID:21346195

    Open questions at the time
    • Determinants of mitotic centromere targeting independent of HP1 not fully enumerated here
  11. 2011 Medium

    Genetically positioned Sgo1 within a Bub1-Sgo1-Mps1 biorientation pathway acting in parallel to Aurora B, with Mps1 required for Sgo1 kinetochore localization.

    Evidence High-copy suppressor screen and genetic epistasis in S. cerevisiae

    PMID:21389114

    Open questions at the time
    • Direct biochemical Mps1-Sgo1 interaction not demonstrated
    • Genetic-only evidence
  12. 2012 High

    Defined the activating switch: CDK-mediated mitotic phosphorylation enables SGO1 to bind a WAPL-free cohesin complex, where SGO1-PP2A dephosphorylates PDS5-bound sororin to protect centromeric cohesin.

    Evidence In vitro kinase/binding assays, RNAi, and non-phosphorylatable sororin rescue in human cells

    PMID:23242214

    Open questions at the time
    • Structural basis of the phospho-dependent cohesin engagement not resolved
    • How tension reverses this protection not addressed here
  13. 2013 High

    Distinguished two mechanistically separate SGO1-PP2A pools (inner-centromere cohesin-bound and kinetochore H2A-pT120-bound) and showed tension drives their redistribution, linking cohesion protection to faithful segregation.

    Evidence RNAi, phospho-mutant expression, and tension perturbation with imaging in human cells

    PMID:24055156

    Open questions at the time
    • Signal coupling tension to SGO1 dephosphorylation not molecularly defined
  14. 2014 High

    Established by artificial-tethering sufficiency that a principal function of Sgo1 is to recruit PP2A-Rts1 to pericentromeres, and clarified that pre-anaphase chromosomal removal is tension-driven rather than degradation-driven.

    Evidence Artificial centromere targeting and genetic epistasis in S. cerevisiae

    PMID:25236599

    Open questions at the time
    • Whether PP2A recruitment is fully sufficient in mammalian cells not tested here
  15. 2014 Medium

    Extended Sgo1's biorientation role beyond cohesin protection, showing it directs condensin to centromeric chromatin and maintains Aurora B/Ipl1 at kinetochores.

    Evidence Genetic epistasis and ChIP in S. cerevisiae

    PMID:24945276

    Open questions at the time
    • Direct Sgo1-condensin interaction not demonstrated
    • Mammalian relevance untested
  16. 2014 Medium

    Identified Aurora B as a kinase that phosphorylates SGO1 to control its partition between centromeres and chromosome arms.

    Evidence Co-IP, in vitro kinase assay, and phospho-mutant localization in human cells

    PMID:25451264

    Open questions at the time
    • Functional consequence for cohesion vs biorientation not separated
    • Phospho-sites not detailed in this entry
  17. 2014 Medium

    Linked SGOL1 to human disease by identifying the K23E founder mutation causing CAID syndrome, with patient cells showing centromeric cohesion defects and zebrafish phenocopy.

    Evidence Human genetics, patient fibroblast phenotyping, and zebrafish morpholino knockdown

    PMID:25282101

    Open questions at the time
    • Molecular mechanism by which K23E impairs SGO1 function not established
    • Connection between cohesion defect and enteric/smooth muscle phenotype unclear
  18. 2015 High

    Showed SGO1 directly reads the Bub1-dependent H2A-pT120 mark and that Bub1-driven mitotic transcription by Pol II is needed for kinetochore-bound SGO1 to access cohesin in centromeric chromatin.

    Evidence Co-IP, in vitro RNA-binding, Pol II inactivation, and ChIP in human cells

    PMID:26190260

    Open questions at the time
    • How transcription physically delivers SGO1 to cohesin not resolved
    • RNA partner identity undefined
  19. 2016 High

    Demonstrated that CENP-C and CENP-T independently route SGO1 to centromeres via Bub1 recruitment, and that forced centromeric Bub1 alone restores SGO1 localization, identifying H2A phosphorylation as the convergent signal.

    Evidence Xenopus cell-free reconstitution with immunodepletion and artificial targeting

    PMID:27116032

    Open questions at the time
    • Quantitative contribution of each inner-kinetochore arm not parsed
  20. 2016 Medium

    Showed the Bub1-H2A-Sgo1-PP2A-Rts1 axis prevents premature spindle checkpoint silencing before tension is generated, coupling SGO1 to checkpoint timing.

    Evidence Systematic genetic epistasis with defined mutants in S. cerevisiae

    PMID:28040741

    Open questions at the time
    • Direct checkpoint substrate of the axis not identified
    • Yeast-specific
  21. 2016 Medium

    Defined a structural role for the Bub1/Sgo1 module as a rheostat reshaping pericentric chromatin in response to microtubule dynamics.

    Evidence Quantitative live-cell imaging and genetics in S. cerevisiae

    PMID:22365852

    Open questions at the time
    • Mechanism connecting chromatin spring changes to cohesion not established
    • Mammalian conservation untested
  22. 2018 Medium

    Identified CENP-A as a Sgo1 interactor in yeast, with the Sgo1 N-terminal coiled-coil sufficient for CENP-A binding and core-centromere association required for genome stability.

    Evidence In vivo/in vitro Co-IP, domain deletion, ChIP, and chromosome loss assays in S. cerevisiae

    PMID:28980861

    Open questions at the time
    • Whether CENP-A binding is direct vs bridged not fully resolved
    • Single lab
  23. 2019 High

    Defined SET as a direct negative regulator that disrupts the SGO1-cohesin interaction to enable timely segregation, identifying an antagonist of cohesion protection.

    Evidence In vitro binding, mutagenesis, overexpression/depletion, and live-cell imaging in human cells

    PMID:31227592

    Open questions at the time
    • How SET activity is regulated during the cell cycle not addressed
  24. 2020 High

    Showed SGO1 binds the Borealin dimerization domain directly and simultaneously with PP2A, and that this interaction drives CPC chromosomal recruitment and Aurora B-dependent spindle assembly but not SAC signaling.

    Evidence Xenopus egg extract reconstitution, direct binding, and mutagenesis

    PMID:32697622

    Open questions at the time
    • Coordination between Borealin and Survivin contacts not fully integrated here
  25. 2022 High

    Defined the histone-H3-like SGO1 N-terminal tail-Survivin BIR domain interaction as the essential hotspot for CPC-SGO1 assembly, with SGO1 and H3T3ph competing for the same Survivin surface.

    Evidence Integrative structure-function analysis, mutagenesis, and Co-IP in human cells

    PMID:35776132

    Open questions at the time
    • How competition with H3T3ph is regulated in time/space not resolved
  26. 2024 Medium

    Established in human cells that basic C-terminal SGO1 residues mediate CENP-A binding and that this interaction determines SGO1 and Aurora B centromere localization and segregation fidelity.

    Evidence Co-IP, site-directed charge-reversal mutagenesis, and segregation assays in human cells

    PMID:37777834

    Open questions at the time
    • Whether CENP-A binding is direct not fully resolved
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular signal that couples kinetochore tension to SGO1 dephosphorylation and redistribution, and the mechanism by which the CAID-causing K23E substitution disrupts SGO1 function, remain unresolved.
  • No structural model of tension-driven SGO1 conformational/positional change
  • No mechanistic explanation of K23E pathogenicity
  • Identity of the SGO1-associated RNA enabling cohesin access undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0042393 histone binding 3 GO:0098772 molecular function regulator activity 2 GO:0003723 RNA binding 1
Localization
GO:0005694 chromosome 4 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1643685 Disease 1
Partners
BOREALINBUB1CENP-ANEK2APLK1PP2ASETSURVIVIN
Complex memberships
SGO1-PP2Achromosomal passenger complex (CPC)cohesin

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 Human SGO1 directly binds to protein phosphatase 2A (PP2A). PP2A localizes to centromeres in a Bub1-dependent manner, and the SGO1-PP2A interaction is required for centromeric localization of SGO1. Depletion of Plk1 by RNAi restores centromeric SGO1 localization in PP2A-depleted cells, indicating that Bub1 targets PP2A to centromeres which counteracts Plk1-mediated removal of SGO1. Co-immunoprecipitation, RNAi depletion, immunofluorescence localization, epistasis analysis Developmental cell High 16580887
2012 CDK-mediated mitosis-specific phosphorylation of SGO1 activates its cohesion-protection function and enables direct binding of SGO1 to cohesin. The phospho-SGO1-bound cohesin complex contains PP2A, PDS5, and hypophosphorylated sororin, but lacks WAPL. SGO1-PP2A dephosphorylates PDS5-bound sororin, protecting centromeric cohesin from WAPL. Expression of non-phosphorylatable sororin bypasses the requirement for SGO1-PP2A in centromeric cohesion. In vitro kinase assay, co-immunoprecipitation, RNAi, phospho-mutant expression, rescue experiments Nature cell biology High 23242214
2015 SGO1 is a direct reader of the Bub1-mediated histone H2A-pT120 mark. Bub1 recruits RNA Polymerase II (Pol II) to unattached kinetochores, promoting mitotic transcription. Mitosis-specific inactivation of Pol II traps SGO1 at kinetochores and weakens centromeric cohesion. SGO1 interacts with Pol II in human cells and with RNA in vitro, suggesting Pol II-dependent transcription enables kinetochore-bound SGO1 to reach cohesin embedded in centromeric chromatin. Co-immunoprecipitation, RNA-binding assay in vitro, Pol II inactivation, immunofluorescence, ChIP Molecular cell High 26190260
2013 Cohesin and histone H2A-pT120 specify two distinct pools of SGO1-PP2A: one at inner centromeres (cohesin-bound) and one at kinetochores (H2A-pT120-bound). Bub1 inactivation delocalizes cohesin-SGO1 to chromosome arms. Kinetochore tension triggers SGO1 dephosphorylation and redistributes SGO1 from inner centromeres to kinetochores; incomplete redistribution causes chromosome nondisjunction. RNAi, phospho-mutant expression, immunofluorescence, tension perturbation experiments Current biology : CB High 24055156
2005 Budding yeast Sgo1 is required for sensing lack of tension at the kinetochore in mitosis. SGO1 mutant cells respond normally to microtubule depolymerization but fail to respond to lack of tension, and have difficulty attaching sister chromatids to opposite poles (biorientation defect). Degradation of Sgo1 when sister chromatids separate prevents cell cycle arrest in anaphase. Genetic mutant analysis, spindle checkpoint assays, chromosome segregation assays in S. cerevisiae Science (New York, N.Y.) High 15637284
2009 In Xenopus egg extract reconstitution, Sgo1 plays a role in stabilizing cohesin along chromosome arms during prophase. This stabilizing activity is antagonized by the mitotic kinases Plk1 and Aurora B, which promotes sister chromatid resolution. Xenopus egg extract in vitro reconstitution, immunodepletion, functional rescue Genes & development High 19696148
2008 The short splice variant of SGO1 (sSgo1) localizes to the centrosome in interphase and to spindle poles in mitosis. sSgo1 interacts with Plk1, and its spindle pole localization is Plk1-dependent. sSgo1 is required for centriole cohesion; its depletion causes separation of paired centrioles, and this can be suppressed by sSgo1 re-expression or Plk1 knockdown. GFP localization, co-immunoprecipitation, RNAi, dominant-negative mutant expression, centrosome imaging Developmental cell High 18331714
2007 NEK2A directly binds human SGO1 in vitro and co-localizes with SGO1 at kinetochores. NEK2A phosphorylates SGO1 at Ser14 and Ser507. Non-phosphorylatable SGO1 mutants perturb chromosome congression and cause increased microtubule attachment errors (syntelic and monotelic attachments), though phosphorylation is not required for SGO1 assembly at kinetochores. In vitro pulldown, in vitro phosphorylation assay with 32P incorporation, phospho-site mapping, expression of phospho-mutants, immunofluorescence Cell research High 17621308
2014 In budding yeast, Sgo1 together with PP2A-Rts1 ensures localization of condensin to centromeric chromatin. Sgo1 is also required for maintaining Aurora B/Ipl1 localization on kinetochores during metaphase. Thus Sgo1 has a dual function in promoting chromosome biorientation: modulating pericentric chromatin conformation via condensin, and maintaining Aurora B/Ipl1 at kinetochores. Genetic epistasis, chromatin immunoprecipitation (ChIP), immunofluorescence, yeast genetics PLoS genetics Medium 24945276
2008 Human SGO1 is degraded during mitotic exit and is a substrate of APC/C-Cdh1. SGO1 contains both a KEN box and a D-box; deletion of either motif alone is insufficient to stabilize SGO1, but simultaneous deletion of both renders it stable. Non-degradable SGO1 does not impair mitotic progression or sister-chromatid separation, indicating SGO1 degradation is not required for these processes. Bub1 contributes to SGO1 steady-state levels via an APC/C-independent mechanism. Ubiquitination assay in vitro, overexpression/knockdown, deletion mutagenesis, cell cycle synchronization, western blot The Journal of biological chemistry High 19015261
2005 In budding yeast, Sgo1 localizes to cohesin-associated regions (CARs) at the centromere and a 50-kb pericentric domain during meiosis I. Establishment of this domain requires the 120-bp core centromere, Bub1, and the meiosis-specific factor Spo13. Cohesins and kinetochore proteins Iml3/Chl4 are necessary for Sgo1 to associate with pericentric but not core centromeric regions. This 50-kb Sgo1-binding domain is where cohesins are protected from removal during meiosis I. ChIP, genetic epistasis, localization studies in S. cerevisiae meiosis Genes & development High 16357219
2010 SGO1 is first recruited to centromeric heterochromatin in G2 in an HP1-dependent manner that does not require Bub1 kinase activity. In prophase, SGO1 becomes diffusely localized, then returns to centromeres in a Bub1 kinase-dependent manner. Despite the absence of SGO1 from mitotic centromeres when Bub1 kinase is absent, centromeric cohesion is maintained, suggesting the G2 recruitment establishes the initial protection mechanism. BUB1-null MEF complementation assay, immunofluorescence, kinase-dead Bub1 expression, cell cycle staging Journal of cell science Medium 20124418
2011 HP1α is targeted to mitotic centromeres by INCENP (a CPC subunit), not by SGO1. Both HP1-INCENP and HP1-SGO1 interactions require the HP1 chromo shadow domain binding to PXVXL/I motifs. An SGO1 mutant deficient in HP1 binding is fully functional in centromeric cohesion protection and localizes normally to centromeres in mitosis. HP1 binding by SGO1 is required for interphase centromere localization but dispensable for mitotic cohesion protection. Biochemical and structural analysis, mutagenesis, complementation assay, immunofluorescence in human cells Molecular biology of the cell High 21346195
2011 In budding yeast, overexpression of SGO1 partially corrects chromosome segregation defects of bub1Δ cells and restores viability to bub1Δ tetraploid cells. Overexpression of CPC subunits Bir1 (survivin) and Sli15 (INCENP) suppresses growth defects of both bub1Δ and sgo1Δ tetraploids. Sgo1 is identified as a possible partner of Mps1; Mps1 is required for Sgo1 kinetochore localization, and Sgo1 overexpression rescues Mps1 inactivation defects. The Bub1-Sgo1-Mps1 pathway acts independently of Aurora B for biorientation. High-copy suppressor screen, genetic epistasis, yeast genetics, overexpression rescue experiments Molecular biology of the cell Medium 21389114
2014 In budding yeast, Sgo1 recruits the PP2A isoform containing Rts1 to the pericentromeric region prior to biorientation. Artificial recruitment of Rts1 to a single chromosome is sufficient to perform the function of Sgo1 on that chromosome, establishing that a major function of Sgo1 is to recruit PP2A-Rts1. Sgo1 is also an APC/C substrate in yeast, with mitotic destruction depending on a D-box-related motif; however, removal from chromosomes before anaphase depends on tension-responsive mechanisms rather than degradation. Yeast genetics, artificial centromere targeting, epistasis, cell cycle analysis Journal of cell science High 25236599
2014 Aurora B kinase interacts with and phosphorylates SGO1 in vitro and in vivo. Aurora B-mediated phosphorylation regulates the distribution of SGO1 between centromeres and chromosome arms. Expression of Aurora B kinase-dead mutant forms of SGO1 causes mislocalization from centromeres to chromosome arms. Co-immunoprecipitation, in vitro kinase assay, phospho-mutant expression, immunofluorescence Biochemical and biophysical research communications Medium 25451264
2022 Using an integrative structure-function approach, the 'histone H3-like' SGO1 N-terminal tail interaction with the Survivin BIR domain was identified as the hotspot essential for CPC-SGO1 assembly, with downstream SGO1 residues and Borealin contributing to high-affinity binding. Disrupting the SGO1-Survivin interaction abolishes CPC-SGO1 assembly and perturbs CPC centromere localization and function. SGO1 and H3T3ph use the same surface on Survivin to bind CPC. Structural analysis, mutagenesis, co-immunoprecipitation, functional assays in human cells The Journal of cell biology High 35776132
2018 Budding yeast CENP-A (Cse4) interacts with SGO1 in vivo and in vitro. The N-terminus coiled-coil domain of Sgo1 (without the C-terminus) is sufficient for interaction with CENP-A and association with core centromeric (CEN) but not pericentric chromatin in a cell cycle-dependent manner. Depletion of CENP-A results in loss of Sgo1 from centromeric chromatin. The N-terminus is required for genome stability. Co-immunoprecipitation in vivo and in vitro, domain deletion analysis, ChIP, chromosome loss assay in S. cerevisiae Cell cycle (Georgetown, Tex.) Medium 28980861
2020 SGO1 interacts directly with the dimerization domain of the CPC subunit Borealin in Xenopus egg extracts. Borealin and PP2A can bind simultaneously to the coiled-coil domain of SGO1. A Borealin mutant that disrupts SGO1-Borealin interaction causes defects in CPC chromosomal recruitment and Aurora B-dependent spindle assembly but not in spindle assembly checkpoint signaling at unattached kinetochores. Xenopus egg extract biochemical reconstitution, direct binding assay, mutagenesis, immunodepletion, functional assays Molecular biology of the cell High 32697622
2016 In budding yeast, the Bub1-H2A-Sgo1-PP2A-Rts1 axis prevents premature silencing of the spindle assembly checkpoint prior to tension generation. Mutation of Bub1's kinase domain, the Bub1 phosphorylation site in H2A, or sgo1 mutants with abolished binding to H2A or PP2A-Rts1 all result in premature SAC silencing in the presence of tensionless attachment. Genetic epistasis, yeast mutant analysis, checkpoint assays in S. cerevisiae Genetics Medium 28040741
2019 SET directly binds to a domain in SGO1 in close proximity to the cohesin-binding motif. SET disrupts SGO1-cohesin binding in a dose-dependent manner in vitro and by SET overexpression in cells. SET binding-deficient SGO1 mutant fully supports centromeric cohesion but delays chromosome segregation. SET depletion delays cohesion fatigue, and overexpression exacerbates it. Thus, SET functions during mitosis by disrupting the SGO1-cohesin interaction to promote timely chromosome segregation. In vitro binding assay, co-immunoprecipitation, mutagenesis, overexpression/depletion, live-cell imaging The Journal of cell biology High 31227592
2024 CENP-A interacts with SGO1 and determines the localization of SGO1 to the centromere during mitosis. Lysine and arginine residues in the C-terminal domain of SGO1 are critical for CENP-A binding. Replacement of these basic residues with acidic residues perturbs localization of SGO1 and Aurora B to the centromere, resulting in aberrant chromosome segregation and premature chromatid separation. Co-immunoprecipitation, site-directed mutagenesis, immunofluorescence in human cells, functional chromosome segregation assay Journal of molecular cell biology Medium 37777834
2016 In budding yeast, Bub1 and Sgo1 act as a rheostat to regulate pericentric chromatin structure. Histone H2A phosphorylation by Bub1 and subsequent Sgo1 recruitment softens the chromatin spring in response to changes in microtubule dynamics. In response to reduced microtubule dynamics, a Bub1/Sgo1-dependent structural change causes contraction of inner kinetochore clusters and radial expansion of pericentric chromatin, with the pericentric chromatin becoming less dynamic. Live-cell imaging, fluorescence imaging, genetic analysis in S. cerevisiae, quantitative chromatin dynamics measurements Current biology : CB Medium 22365852
2006 Two major isoforms of human SGO1 exhibit distinct subcellular localization: the longer isoform localizes to kinetochores during G2 through mitosis, while the short isoform (sSgo1, lacking exon 6) does not localize to kinetochores but enriches at mitotic spindles and is found at S-phase foci (some colocalizing with kinetochores). These different localizations suggest isoform-specific functions during the cell cycle. GFP fusion localization, live-cell imaging, co-localization with CREST antigen Cell cycle (Georgetown, Tex.) Medium 16582621
2016 In Xenopus cell-free system, both CENP-C and CENP-T can independently drive centromeric accumulation of SGO1 through recruitment of Bub1 to the KMN network. Mps1 is also required for this pathway. Forced targeting of Bub1 to centromeres (in the absence of other kinetochore components) is sufficient to restore SGO1 localization, demonstrating that Bub1-mediated H2A phosphorylation is the critical downstream signal. Xenopus egg cell-free system, immunodepletion, artificial targeting experiments, immunofluorescence Chromosoma High 27116032
2014 A homozygous founder mutation in SGOL1 (K23E) causes CAID syndrome. Cultured fibroblasts from affected individuals showed accelerated cell cycle progression, higher senescence rate, and enhanced TGF-β signaling. Karyotypes showed centromeric cohesion defect (railroad appearance). Morpholino-induced knockdown of sgol1 in zebrafish recapitulated the human abnormalities including defects in enteric nervous system and smooth muscle. Human genetics, cell biological analysis of patient fibroblasts, zebrafish morpholino knockdown, karyotyping Nature genetics Medium 25282101
2012 In budding yeast, Bub1 kinase activity is not required for spindle checkpoint function but is required for centromeric localization of Sgo1. Despite absence of centromeric Sgo1 in mitotic cells lacking Bub1 kinase activity, centromeric cohesion is still maintained until anaphase (negative result: Bub1 kinase-dependent Sgo1 centromeric localization in mitosis is not required to maintain cohesion). Bub1 kinase-dead MEF complementation, immunofluorescence, functional cohesion assays Journal of cell science (duplicate entry under PMID 20124418) Medium 20124418

Source papers

Stage 0 corpus · 55 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation. Developmental cell 283 16580887
2009 Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1. Genes & development 176 19696148
2012 Phosphorylation-enabled binding of SGO1-PP2A to cohesin protects sororin and centromeric cohesion during mitosis. Nature cell biology 162 23242214
2005 The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes. Science (New York, N.Y.) 154 15637284
2015 Mitotic Transcription Installs Sgo1 at Centromeres to Coordinate Chromosome Segregation. Molecular cell 148 26190260
2013 Phospho-H2A and cohesin specify distinct tension-regulated Sgo1 pools at kinetochores and inner centromeres. Current biology : CB 124 24055156
2008 sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1. Developmental cell 106 18331714
2007 Bub1 kinase targets Sgo1 to ensure efficient chromosome biorientation in budding yeast mitosis. PLoS genetics 97 18081426
2014 Mutations in SGOL1 cause a novel cohesinopathy affecting heart and gut rhythm. Nature genetics 94 25282101
2011 Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells. Molecular biology of the cell 79 21346195
2005 The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I. Genes & development 79 16357219
2008 Human Sgo1 downregulation leads to chromosomal instability in colorectal cancer. Gut 66 18635744
2012 Haploinsufficiency of SGO1 results in deregulated centrosome dynamics, enhanced chromosomal instability and colon tumorigenesis. Cell cycle (Georgetown, Tex.) 58 22262168
2014 Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation. PLoS genetics 56 24945276
2007 Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis. Cell research 46 17621308
2012 Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics. Current biology : CB 44 22365852
2008 Multiple anaphase-promoting complex/cyclosome degrons mediate the degradation of human Sgo1. The Journal of biological chemistry 41 19015261
2018 Genome-wide CRISPR screen reveals SGOL1 as a druggable target of sorafenib-treated hepatocellular carcinoma. Laboratory investigation; a journal of technical methods and pathology 40 29467456
2011 Bub1, Sgo1, and Mps1 mediate a distinct pathway for chromosome biorientation in budding yeast. Molecular biology of the cell 40 21389114
2010 Sgo1 establishes the centromeric cohesion protection mechanism in G2 before subsequent Bub1-dependent recruitment in mitosis. Journal of cell science 37 20124418
2014 Sgo1 recruits PP2A to chromosomes to ensure sister chromatid bi-orientation during mitosis. Journal of cell science 32 25236599
2006 Differential subcellular localizations of two human Sgo1 isoforms: implications in regulation of sister chromatid cohesion and microtubule dynamics. Cell cycle (Georgetown, Tex.) 32 16582621
2011 A novel tumor-derived SGOL1 variant causes abnormal mitosis and unstable chromatid cohesion. Oncogene 30 21532624
2015 Sgo1 is a potential therapeutic target for hepatocellular carcinoma. Oncotarget 28 25638162
2013 Frameshift mutations of chromosome cohesion-related genes SGOL1 and PDS5B in gastric and colorectal cancers with high microsatellite instability. Human pathology 26 23850494
2013 SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small cell lung cancers. Scientific reports 24 24146025
2011 Sgo1 is required for co-segregation of sister chromatids during achiasmate meiosis I. Cell cycle (Georgetown, Tex.) 23 21330786
2016 Systemic Chromosome Instability Resulted in Colonic Transcriptomic Changes in Metabolic, Proliferation, and Stem Cell Regulators in Sgo1-/+ Mice. Cancer research 20 26833665
2015 Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. Carcinogenesis 20 25740822
2021 Long noncoding RNA SGO1-AS1 inactivates TGFβ signaling by facilitating TGFB1/2 mRNA decay and inhibits gastric carcinoma metastasis. Journal of experimental & clinical cancer research : CR 19 34706749
2017 The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1. Oncotarget 19 29100415
2022 Mechanistic basis for Sgo1-mediated centromere localization and function of the CPC. The Journal of cell biology 17 35776132
2018 Budding yeast CENP-ACse4 interacts with the N-terminus of Sgo1 and regulates its association with centromeric chromatin. Cell cycle (Georgetown, Tex.) 17 28980861
2019 SGO1 induces proliferation and metastasis of prostate cancer through AKT-mediated signaling pathway. American journal of cancer research 16 31911855
2016 SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Scientific reports 16 27539729
2006 Human Sgo1 is an excellent target for induction of apoptosis of transformed cells. Cell cycle (Georgetown, Tex.) 16 16628005
2020 The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly. Molecular biology of the cell 15 32697622
2011 Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 15 22161216
2018 Molecular Signature of CAID Syndrome: Noncanonical Roles of SGO1 in Regulation of TGF-β Signaling and Epigenomics. Cellular and molecular gastroenterology and hepatology 13 30739867
2016 Bub1 targeting to centromeres is sufficient for Sgo1 recruitment in the absence of kinetochores. Chromosoma 13 27116032
2016 Premature Silencing of the Spindle Assembly Checkpoint Is Prevented by the Bub1-H2A-Sgo1-PP2A Axis in Saccharomyces cerevisiae. Genetics 13 28040741
2014 Regulation of the subcellular shuttling of Sgo1 between centromeres and chromosome arms by Aurora B-mediated phosphorylation. Biochemical and biophysical research communications 10 25451264
2019 Assessment of SGO1 and SGO1-AS1 contribution in breast cancer. Human antibodies 9 31156154
2019 SET binding to Sgo1 inhibits Sgo1-cohesin interactions and promotes chromosome segregation. The Journal of cell biology 8 31227592
2017 Characterization of Sgo1 expression in developing and adult mouse. Gene expression patterns : GEP 8 28465207
2015 Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. Molecular carcinogenesis 8 25773652
2013 SGO1 maintains bovine meiotic and mitotic centromeric cohesions of sister chromatids and directly affects embryo development. PloS one 8 24019931
2017 Dataset of Sgo1 expression in cardiac, gastrointestinal, hepatic and neuronal tissue in mouse. Data in brief 6 28748208
2022 The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development. Developmental dynamics : an official publication of the American Association of Anatomists 5 35275424
2024 Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis. Journal of molecular cell biology 4 37777834
2022 SGOL1-AS1 enhances cell survival in acute myeloid leukemia by maintaining pro-inflammatory signaling. Heliyon 4 36387443
2016 Haplo-insufficiency of both BubR1 and SGO1 accelerates cellular senescence. Journal of hematology & oncology 4 26847209
2009 Suppression of genomic instabilities caused by chromosome mis-segregation: a perspective from studying BubR1 and Sgo1. Journal of the Formosan Medical Association = Taiwan yi zhi 3 20040454
2025 Chronic atrial and intestinal dysrythmia syndrome: A late-onset intestinal pseudo-obstruction and cardiac dysfunction due to an SGO1 mutation. JPGN reports 0 41245052
2020 Microsatellite frameshift variants in SGO1 of gastric cancer are not always associated with MSI status. Journal of clinical pathology 0 32817265

Missed literature

Know a paper Affinage missed for SGO1? Flag it for the maintainers and the community.

No submissions yet.