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Showing RUVBL1 — RVB1 is a alias.

RUVBL1

RuvB-like 1 · UniProt Q9Y265

Length
456 aa
Mass
50.2 kDa
Annotated
2026-06-10
100 papers in source corpus 52 papers cited in narrative 51 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RUVBL1 is an essential AAA+ ATPase that pairs with RUVBL2 to form heterohexameric and stacked dodecameric ring structures, serving as a scaffold and assembly chaperone for numerous nuclear and cytoplasmic multiprotein machines (PMID:10428817, PMID:17157868, PMID:9774387). Each protomer comprises ATP-binding/hydrolysis domains (I and III) and a eukaryote-specific, nucleic-acid-binding domain II that auto-inhibits and allosterically gates ATPase and helicase activity through large conformational transitions (PMID:17060327, PMID:21933716, PMID:23002137); client proteins and cofactors bind domain II to license catalysis and remodel oligomeric state, as shown for Ino80INS, the R2TP factors PIH1D1 and RPAP3, DHX34, NOPCHAP1, and DPCD (PMID:28591576, PMID:31049401, PMID:33205750, PMID:33367824, PMID:35901867). Although isolated RUVBL1 was first characterized as an ssDNA-stimulated 3'-to-5' ATP-dependent DNA helicase, the assembled RUVBL1/2 complex shows only marginal intrinsic helicase activity and acts principally as an assembly chaperone whose ATPase cycle drives client loading and complex maturation (PMID:10336418, PMID:17157868, PMID:31883965). Through this activity RUVBL1/2 builds and regulates chromatin-modifying and remodeling complexes—notably enabling TIP60/NuA4 histone acetyltransferase activity and ATPase-dependent H2A.Z exchange into nucleosomes (PMID:18285460, PMID:19696079)—and these functions underpin DNA double-strand break repair by both homologous recombination and non-homologous end joining, with PRMT5-mediated R205 methylation of RUVBL1 promoting TIP60 HAT activity, H4K16 acetylation and 53BP1 displacement to favor HR, and DTL-mediated ubiquitination shifting the balance toward NHEJ (PMID:28238654, PMID:18834951, PMID:38609375). RUVBL1/2 is the assembly engine of broader R2TP/PAQosome chaperone activity required for PIKK stability and nonsense-mediated decay, mTORC1 dimerization and lysosomal localization, U5 snRNP and C/D snoRNP biogenesis, the Fanconi anemia core complex, and the γ-tubulin ring complex, and it supports mitotic spindle function and ciliogenesis (PMID:20371770, PMID:23142078, PMID:28561026, PMID:33367824, PMID:25428364, PMID:33355144, PMID:26201077, PMID:29228333, PMID:29959317). In transcription it acts as a cofactor for the β-catenin/TCF, MYC and E2F1 programs and, with cytoplasmic disaggregase activity toward amyloid and aggregated substrates, links the complex to proteostasis (PMID:9843967, PMID:11080158, PMID:14695187, PMID:37075745, PMID:26303906). RUVBL1/2 ATPase activity is required for cancer cell proliferation and is druggable by the allosteric inhibitor CB-6644, with MYC-driven tumors being acutely dependent on RUVBL1 (PMID:30640450, PMID:38821858).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1998 High

    Established that RUVBL1 is a nuclear, RuvB-homologous protein physically linked to transcription machinery and essential for viability, framing it as a conserved enzyme with a core cellular role.

    Evidence Yeast two-hybrid and Co-IP placing it in the RNA Pol II holoenzyme, plus a lethal yeast null mutation; parallel work mapping its binding to β-catenin and TBP

    PMID:9196036 PMID:9774387 PMID:9843967

    Open questions at the time
    • Did not define the biochemical activity of the protein
    • Did not identify the obligate RUVBL2 partner
  2. 1999 High

    Resolved the biochemical activity by showing recombinant RUVBL1 is an ATP-dependent 3'-to-5' DNA helicase and that it forms a ~700 kDa complex with the opposite-polarity, non-redundant RUVBL2.

    Evidence In vitro ATPase/helicase assays on purified protein, Co-IP, gel filtration, and yeast complementation

    PMID:10336418 PMID:10428817

    Open questions at the time
    • Helicase activity later not detected in the reconstituted human complex
    • Physiological substrate of the helicase activity unknown
  3. 2000 High

    Showed RUVBL1 and RUVBL2 act antagonistically on β-catenin/TCF transactivation, establishing the paralog pair as functionally distinct regulators of Wnt-pathway transcription conserved to Drosophila.

    Evidence Reporter transactivation assays, Co-IP, and Drosophila genetics

    PMID:11080158

    Open questions at the time
    • Mechanism of antagonism at chromatin not defined
    • Did not link to a specific chromatin-modifying complex
  4. 2003 High

    Connected RUVBL1 ATPase activity to chromatin-templated transcription, showing it operates within TIP60/TRRAP/BAF53 complexes to drive histone acetylation and β-catenin-mediated transformation.

    Evidence ATPase-dead D302N mutant, ChIP at the ITF-2 promoter, siRNA, and Co-IP; plus centrosome/spindle association and tubulin assembly assays

    PMID:14506706 PMID:14695187

    Open questions at the time
    • Did not separate scaffolding from catalytic contributions to acetylation
    • Mitotic role only correlative at this stage
  5. 2008 High

    Defined a complex-specific requirement: RUVBL1 is needed for TIP60/NuA4 HAT activity (not INO80 or SRCAP) and links chromatin acetylation to DNA damage resolution, while the yeast Rvb1/Rvb2 hexamer was shown to be an enhanced ATPase/helicase.

    Evidence siRNA with in vitro HAT assays, epistasis between Rvb1/Tip60/Ino80/SRCAP, phospho-H2AX persistence, and RAD51 foci with HDAC-inhibitor rescue; in vitro reconstitution of yeast heterohexamer

    PMID:18234224 PMID:18285460 PMID:18834951

    Open questions at the time
    • Mechanism coupling acetylation to phospho-H2AX dephosphorylation incomplete
    • Direct enzymatic role in HR not isolated from chromatin remodeling
  6. 2009 High

    Demonstrated the catalytic mechanism by which the TIP48/TIP49 ATPases drive TIP60-acetylation-dependent H2A.Z deposition into nucleosomes, defining a histone-exchange function.

    Evidence Purification of H2A.Z-interacting complexes with in vitro exchange and TIP60 HAT assays and ATPase-dependent dissection

    PMID:19696079

    Open questions at the time
    • Structural basis of nucleosome engagement not resolved
    • In vivo locus-specificity of exchange not addressed
  7. 2011 High

    Established the structural logic of regulation: dodecameric assembly with domain II auto-inhibiting helicase activity, indicating in vivo function is gated by cofactors acting on DII.

    Evidence Crystallography of dodecamer with truncated DII, SAXS, and ATPase/helicase assays; earlier 2.2 Å hexamer structure defining DII as the nucleic-acid-binding domain

    PMID:17060327 PMID:17157868 PMID:21933716

    Open questions at the time
    • Identity of physiological DII-acting cofactors not yet defined
    • Link between conformational state and specific complex assembly unclear
  8. 2012 High

    Established RUVBL1/2 as the core of the R2TP-related TTT chaperone that assembles PIKKs and mTORC1, coupling complex assembly to nutrient/energy status, and visualized the DII-driven conformational equilibrium.

    Evidence Co-IP across PIKK family members with NMD readout, TTT-mTORC1 fractionation/epistasis and metabolic flux assays, Hsp90 inhibition, and cryo-EM of compact/stretched states

    PMID:20371770 PMID:21951644 PMID:23002137 PMID:23142078

    Open questions at the time
    • Stoichiometry of client handoff to mature complexes unresolved
    • How energy status mechanically disassembles the complex not defined
  9. 2014 High

    Broadened the assembly-chaperone role to the Fanconi anemia core complex and DNA crosslink repair, with in vivo knockout phenotypes, and implicated YY1 and cytoplasmic actin in additional functions.

    Evidence AP-MS/Co-IP with FA core, siRNA co-depletion, mouse conditional Ruvbl1 knockout causing aplastic anemia, plus YY1 EM/HR assays and F-actin binding/invasion assays

    PMID:24728183 PMID:24990942 PMID:25428364

    Open questions at the time
    • Whether RUVBL1/2 directly chaperones FA assembly versus stabilizes it not fully separated
    • Cytoplasmic actin and YY1 roles rest on single-lab evidence
  10. 2017 High

    Identified post-translational control of RUVBL1 in DNA repair, with PRMT5-mediated R205 methylation enabling TIP60 HAT activity and HR, and resolved client-driven dodecamerization mechanistically via Ino80INS.

    Evidence In vitro methyltransferase assay with R205 mutant, HAT/ChIP assays for H4K16Ac and 53BP1; cryo-EM/XL-MS of Ino80INS-stimulated Rvb1/2; AP-MS linking R2TP to U5 snRNP via ZNHIT2

    PMID:28122980 PMID:28238654 PMID:28561026 PMID:28591576

    Open questions at the time
    • Enzymes reversing R205 methylation unknown
    • How DII-binding clients select among many complexes not defined
  11. 2019 High

    Defined the general DII-gated nucleotide-exchange mechanism and validated RUVBL1/2 ATPase as a druggable, essential activity for tumor growth and DNA replication.

    Evidence Cryo-EM of R2TP (RPAP3-PIH1D1) showing DII-induced gatekeeper destabilization; CB-6644 allosteric inhibition with on-target resistance mutations and xenograft regression; PAQosome maturation and replication-catastrophe assays; C-RAF S259 pathway work

    PMID:29545175 PMID:30640450 PMID:31049401 PMID:31883965

    Open questions at the time
    • How a single allosteric mechanism selectively affects diverse client complexes unresolved
    • Replication phenotype mixes direct and transcription-coupled effects
  12. 2020 High

    Extended the assembly-chaperone paradigm to γTuRC assembly, ciliary complex pre-assembly, and disaggregase activity, and resolved how the NMD helicase DHX34 down-tunes RUVBL2 ATPase via N-terminal conformational change.

    Evidence γTuRC reconstitution with cryo-EM and nucleation assays; conditional Ruvbl1 knockouts causing renal failure and hydrocephalus with cilia defects; cryo-EM of DHX34-RUVBL1/2; disaggregation/ATPase-stimulation assays with amyloid substrates; transcription-replication interference analysis

    PMID:26303906 PMID:29228333 PMID:29959317 PMID:32846207 PMID:33205750 PMID:33355144

    Open questions at the time
    • How one ATPase serves cytoplasmic disaggregation and nuclear assembly is unclear
    • Selectivity for individual ciliary/spindle clients not defined
  13. 2024 High

    Established RUVBL1 as a MYC-cooperating dependency required for oncogenic and immunoevasive gene programs and oncogenic translation, and added further PTM control (GART K7 methylation, DTL ubiquitination) tuning Wnt signaling and repair-pathway choice.

    Evidence Auxin-degron and shRNA screens with in vivo PDAC regression, CRISPR gene-body MYC-interaction mapping with ChIP, YTHDF1/40S Co-IP-MS with ribosome sequencing; in vitro K7-methyltransferase and DTL-ubiquitination assays with ChIP and pathway readouts

    PMID:34276666 PMID:34880314 PMID:35508542 PMID:35876654 PMID:36107469 PMID:37075745 PMID:37439412 PMID:38609375 PMID:38821858 PMID:38900944

    Open questions at the time
    • Whether MYC dependency reflects direct chromatin scaffolding versus chaperone-mediated complex assembly is unresolved
    • Several PTM/cancer findings rest on single-lab evidence

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single RUVBL1/2 ATPase cycle and DII-gated conformational switch achieve selective, ordered assembly of dozens of distinct nuclear and cytoplasmic client complexes remains the central open mechanistic question.
  • No unifying model linking client identity at DII to a defined ATPase output
  • Rules governing partition between disaggregation, histone exchange, and chaperone roles undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140657 ATP-dependent activity 8 GO:0016787 hydrolase activity 4 GO:0003677 DNA binding 3 GO:0044183 protein folding chaperone 2 GO:0140096 catalytic activity, acting on a protein 2 GO:0003723 RNA binding 1
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 2 GO:0005856 cytoskeleton 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-4839726 Chromatin organization 6 R-HSA-392499 Metabolism of proteins 5 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-162582 Signal Transduction 4 R-HSA-73894 DNA Repair 4 R-HSA-1640170 Cell Cycle 3 R-HSA-8953854 Metabolism of RNA 3 R-HSA-69306 DNA Replication 2
Partners
CTNNB1DHX34DPCDNOPCHAP1PIH1D1RUVBL2TBPTIP60
Complex memberships
Fanconi anemia core complexR2TP/PAQosome (RUVBL1-RUVBL2-RPAP3-PIH1D1)TIP60/NuA4 HAT complexTTT-RUVBL1/2 (mTORC1 assembly)

Evidence

Reading pass · 51 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 RUVBL1 (TIP49) was identified as a TBP-interacting protein that forms a complex with TBP in nuclear extracts, isolated by affinity purification using histidine-tagged TBP. The protein contains Walker A and B motifs and shows structural similarity to bacterial RuvB, suggesting ATP-dependent enzymatic activity. Affinity purification (histidine-tagged TBP), immunoprecipitation, sequence analysis Biochemical and biophysical research communications Medium 9196036
1998 RUVBL1 (Pontin52) was identified as a nuclear protein that binds beta-catenin in the region of Armadillo repeats 2–5 and also binds TBP, forming an in vivo multiprotein complex with beta-catenin and LEF-1/TCF. This implicated RUVBL1 in the nuclear transcriptional function of beta-catenin. Yeast two-hybrid (identification), co-immunoprecipitation (in vivo complex validation), domain mapping Proceedings of the National Academy of Sciences of the United States of America High 9843967
1998 RUVBL1 was identified as a human protein homologous to RuvB; it co-immunoprecipitated with cellular proteins and was detected in the RNA polymerase II holoenzyme complex. Null mutation of the yeast homolog scRUVBL1 was nonviable, demonstrating it is essential for cell survival. Yeast two-hybrid (identification via RPA3 bait), co-immunoprecipitation, multi-step chromatographic purification, yeast null mutation The Journal of biological chemistry High 9774387
1999 RUVBL1 (TIP49a/TIP49) is an ATP-dependent DNA helicase with ssDNA-stimulated ATPase activity that unwinds DNA duplexes in the 3' to 5' direction, as established by in vitro enzyme assays with purified recombinant protein. In vitro ATPase assay, DNA helicase assay, UV cross-linking, recombinant protein purification The Journal of biological chemistry High 10336418
1999 RUVBL1 (TIP49a) and RUVBL2 (TIP49b) bind each other and are found together in the same ~700 kDa complex in cells. TIP49b has opposite DNA helicase polarity (5' to 3') compared to TIP49a (3' to 5'), and TIP49b does not complement the TIP49a yeast null mutation, indicating non-redundant functions. Co-immunoprecipitation, gel filtration (complex size), in vitro ATPase and helicase assays, yeast complementation The Journal of biological chemistry High 10428817
2000 RUVBL1 (Pontin52) and RUVBL2 (Reptin52) interact with each other and both bind beta-catenin and TBP, but act antagonistically on beta-catenin/TCF transactivation in reporter gene assays. This antagonism is conserved in Drosophila (dpontin vs. dreptin in Wingless signaling). Reporter gene assay (transactivation), co-immunoprecipitation, Drosophila in vivo genetics The EMBO journal High 11080158
2001 RUVBL1 (TIP49a) functions as a plasminogen-binding protein on the U937 cell surface via a C-terminal lysine; it binds human plasminogen with a Kd of 0.57 μM and enhances plasminogen activation 8-fold, as demonstrated by ligand blotting and plasminogen activation assays. 2D gel electrophoresis, ligand blotting with 125I-plasminogen, carboxypeptidase B sensitivity assay, kinetic binding assay, plasminogen activation assay The Journal of biological chemistry Medium 11027681
2002 RUVBL1 (TIP49) modulates c-Myc-mediated apoptosis and also binds the E2F1 transactivation domain to modulate E2F1-dependent transforming and apoptotic activities, while the related factor TRRAP does not affect apoptosis. This distinguishes RUVBL1 as a specific cofactor for these transcription factors. Co-immunoprecipitation (E2F1 binding), functional apoptosis assays, dominant-negative mutant analysis Oncogene Medium 12185582
2003 RUVBL1 (TIP49) ATPase activity is required for beta-catenin-mediated neoplastic transformation and activation of TCF-dependent genes. An ATPase-deficient mutant (TIP49D302N) inhibited these activities. RUVBL1 was found in complexes with TIP60, TRRAP, and BAF53 chromatin-remodeling factors at the ITF-2 promoter, and its inhibition reduced histone acetylation near TCF-binding sites. ATPase-deficient mutant (D302N) expression, reporter gene assay, siRNA knockdown, chromatin immunoprecipitation (ChIP), co-immunoprecipitation Cancer research High 14695187
2003 RUVBL1 associates with alpha- and gamma-tubulin and localizes to the centrosome and mitotic spindle during mitosis, where its topology varies with mitotic stage. RUVBL1 promotes in vitro tubulin assembly. Protein affinity chromatography, co-immunoprecipitation, confocal immunofluorescence microscopy, GST pulldown, in vitro tubulin assembly assay Cell motility and the cytoskeleton Medium 14506706
2006 The crystal structure of human RuvBL1 hexamer was solved at 2.2 Å resolution in complex with ADP. The structure revealed three domains: domains I and III mediate ATP binding/hydrolysis, while domain II (unique to eukaryotes, absent from bacterial RuvB) is a novel DNA/RNA-binding domain. RuvBL1 binds single-stranded DNA/RNA and double-stranded DNA, but shows only marginal ATPase activity in isolation, suggesting requirement for cofactors. X-ray crystallography (2.2 Å), DNA binding assays, ATPase activity assay The Journal of biological chemistry High 17060327
2006 Human TIP48 and TIP49 form a stable equimolar dodecameric complex (two stacked hexameric rings) with synergistic ATPase activity. Both proteins are required for ATPase activity (catalytic mutants in either abolish activity). No DNA helicase or branch migration activity was detected in the reconstituted complex. In vitro reconstitution, ATPase assay, ATPase-deficient mutants, negative stain electron microscopy (3D reconstruction at 20 Å) Journal of molecular biology High 17157868
2008 Human RUVBL1 (Rvb1) is required for the histone acetyltransferase (HAT) activity of the Tip60/NuA4 complex but not for Ino80 or SRCAP complexes. RUVBL1 depletion increases persistence of phospho-H2AX after DNA damage, and this phenotype is phenocopied by Tip60 depletion. Histone H4 acetylation by Tip60 is required prior to dephosphorylation of phospho-H2AX. siRNA knockdown, in vitro HAT activity assay, immunofluorescence, H4 acetylation assay, epistasis between Rvb1 and Tip60/Ino80/SRCAP Molecular and cellular biology High 18285460
2008 Yeast Rvb1 and Rvb2 form a heterohexameric ring with enhanced ATPase activity compared to individual proteins; ATPase is further stimulated by dsDNA with 5' or 3' overhangs. The complex exhibits ATP-dependent DNA helicase activity preferring 5' to 3' unwinding. In vitro reconstitution, ATPase assay, electron microscopy, DNA helicase assay Journal of molecular biology High 18234224
2008 RUVBL1 depletion reduced RAD51 recruitment to chromatin and nuclear foci formation after DSBs and interstrand crosslinks by ~50%, without affecting DNA damage checkpoint signaling (H2AX phosphorylation). Histone deacetylase inhibitor treatment restored RAD51 foci, linking RUVBL1 to chromatin modification-dependent homologous recombination repair. siRNA knockdown, RAD51 foci immunofluorescence, chromatin fractionation, HDAC inhibitor rescue experiment The international journal of biochemistry & cell biology Medium 18834951
2009 TIP48 and TIP49 play a major role in catalyzing H2A acetylation-induced H2A.Z exchange into nucleosomes via their ATPase activities. TIP60-mediated acetylation of nucleosomal H2A specifically facilitates the action of the complex containing TIP48/TIP49 in the H2A.Z exchange reaction. Purification of H2A.Z-interacting complexes, in vitro H2A.Z exchange assay, TIP60 HAT assay, ATPase-dependent mechanistic dissection Nucleic acids research High 19696079
2010 RUVBL1 and RUVBL2 associate with each PIKK family member (ATM, ATR, mTOR, DNA-PKcs, SMG-1, TRRAP), control PIKK mRNA and protein abundance upon knockdown, and promote the assembly of SMG-1-containing mRNA surveillance complexes in the cytoplasm during nonsense-mediated mRNA decay (NMD). Co-immunoprecipitation (RUVBL1/2 with PIKKs), siRNA knockdown (PIKK abundance and NMD), mRNP complex assembly assay Science signaling High 20371770
2011 RUVBL1 represses p53 transcription by binding to the p53 promoter, interfering with RNF20/hBRE1-mediated histone H2B monoubiquitination, and promoting PAF1-mediated histone H3K9 trimethylation. This mechanism underlies RUVBL1's ability to block p53-mediated apoptosis downstream of EHF transcription factor activation. ChIP, co-immunoprecipitation, siRNA knockdown, reporter assay, histone modification analysis EMBO reports Medium 21617703
2011 The RuvBL1-RuvBL2 heterodimeric complex forms a dodecamer of two heterohexameric rings with alternating RUVBL1/RUVBL2 subunits bound to ADP/ATP. Truncation of domain II substantially increases ATPase activity, and domain II auto-inhibits helicase activity—showing that in vivo activities are regulated by cofactors via domain II conformational changes. X-ray crystallography (dodecamer with truncated DII), SAXS, ATPase assay, helicase assay Journal of structural biology High 21933716
2011 Hsp90 forms complexes with the RUVBL1/2 complex and the Tel2 complex, and Hsp90 inhibition reduces abundance of all PIKK family members and suppresses PIKK-mediated signaling, demonstrating that Hsp90 regulates PIKKs together with RUVBL1/2. Co-immunoprecipitation, Hsp90 inhibitor treatment, immunoblot for PIKK levels Cancer science Medium 21951644
2012 Cryo-EM of the human RuvBL1-RuvBL2 complex revealed two coexisting conformations (compact and stretched) driven by movements in domain II (DII). DII domains connect the AAA+ core and expose DNA-binding regions, suggesting that these conformational transitions regulate the activity of RUVBL1-RUVBL2-containing complexes. Cryo-electron microscopy (~15 Å), image classification, nucleic acid binding analysis Nucleic acids research High 23002137
2012 The TTT-RUVBL1/2 complex (TELO2-TTI1-TTI2 plus RUVBL1/2) is required for mTORC1 assembly into obligate dimers, its lysosomal localization, and its interaction with Rag GTPases. Energy depletion (loss of ATP from glucose/glutamine) disassembles and represses the TTT-RUVBL complex, thereby impairing mTORC1 function. Co-immunoprecipitation, siRNA knockdown, lysosome fractionation, AMPK/TSC epistasis analysis, metabolic flux assays Molecular cell High 23142078
2013 RuvbL1 and RuvbL2 function as disaggregases: they directly interact with aggregated substrate synphilin-1 near the opening of the central channel of the barrel structure, polypeptides with unfolded structures and amyloid fibrils stimulate RuvbL ATPase activity, and the complex promotes disassembly of protein aggregates and aggresome formation. siRNA screen, Co-IP (synphilin-1 interaction), ATPase stimulation assay (amyloid fibrils), aggregate disaggregation assay, yeast genetic studies The EMBO journal High 26303906
2014 RuvBL1 and RuvBL2 co-purify with the Fanconi anemia (FA) core complex under native conditions, and depletion of RuvBL1-RuvBL2 leads to co-depletion of the FA core complex, causes DNA crosslinker sensitivity, chromosomal instability, and defective FA pathway activation. Conditional Ruvbl1 knockout in mouse hematopoietic cells causes aplastic anemia. Affinity purification-MS, co-immunoprecipitation, siRNA depletion, mouse conditional knockout, DNA crosslinker sensitivity assay Nucleic acids research High 25428364
2014 YY1 oligomers preferentially interact with RUVBL1 (rather than RUVBL2) and DNA binding by YY1 oligomers is enhanced in the presence of RuvBL1-RuvBL2. YY1 and the ATPase activity of RUVBL2 are required for RAD51 foci formation during homologous recombination. Co-immunoprecipitation, electron microscopy, in vitro DNA binding assay, functional HR assay (RAD51 foci) The Journal of biological chemistry Medium 24990942
2014 RUVBL1 localizes to the cytoplasm in pancreatic cancer cells where it directly binds filamentous actin (F-actin), increases G-actin concentration in cell protrusions, promotes peripheral actin polymerization, and thereby drives formation of membrane protrusions to enhance cancer cell invasion. siRNA knockdown, co-immunoprecipitation, confocal microscopy (F-actin colocalization), motility/invasion assay International journal of oncology Medium 24728183
2015 RUVBL1 localizes to structures of the mitotic spindle apparatus in anaphase-to-telophase transition, partially co-localizing with PLK1. PLK1 phosphorylates RUVBL1 (but not RUVBL2) in vitro, and they physically associate in vivo. siRNA knockdown of RuvBL proteins causes chromosome alignment and segregation defects, and the ATPase activity of RUVBL1 is indispensable for cell proliferation. Immunofluorescence microscopy, in vitro kinase assay (PLK1), co-immunoprecipitation, siRNA knockdown, ATPase-deficient mutant complementation assay PloS one Medium 26201077
2015 A domain within the INO80 ATPase subunit (Ino80INS) stimulates Rvb1/2 ATPase activity 16-fold and promotes dodecamerization. Cryo-EM and mass spectrometry show Ino80INS binds asymmetrically at the dodecamerization interface, creating a conformationally flexible dodecamer that collapses into hexamers upon ATP addition, demonstrating chaperone-like cycling behavior. ATPase assay, cryo-EM, crosslinking mass spectrometry, integrative structural modeling Cell reports High 28591576
2017 PRMT5 methylates RUVBL1 at arginine R205, which is required for TIP60-dependent mobilization of 53BP1 from DSBs and promotes homologous recombination. PRMT5-directed methylation of RUVBL1 is critically required for TIP60 acetyltransferase activity and histone H4K16 acetylation, which facilitates 53BP1 displacement. Methylation did not affect ATM activation. In vitro methyltransferase assay (PRMT5 on RUVBL1), mutant RUVBL1 (R205 site), TIP60 HAT activity assay, ChIP (H4K16Ac, 53BP1 foci), siRNA Molecular cell High 28238654
2017 The R2TP/Prefoldin-like complex interacts with the U5 snRNP, mediated primarily by the uncharacterized factor ZNHIT2. ZNHIT2 directly binds RUVBL2's DII domain; disruption of ZNHIT2 or RUVBL2 expression impacts U5 snRNP protein composition, implicating RUVBL1/2 in U5 snRNP assembly. Affinity purification-MS (multi-target), co-immunoprecipitation, siRNA knockdown with snRNP composition analysis Nature communications Medium 28561026
2017 Adenovirus E1A binds RUVBL1 via the C-terminus of E1A and is recruited to RUVBL1-regulated promoters in an interferon-dependent manner, suppressing interferon-stimulated gene transcriptional activation. Depletion of RUVBL1 impairs adenovirus growth but does not affect viral genome replication or S-phase induction. Co-immunoprecipitation (E1A-RUVBL1), ChIP, siRNA knockdown, viral growth assay, domain mapping Journal of virology Medium 28122980
2018 RUVBL1 interacts with DNAAF1 and IFT88 (ciliary intraflagellar transport protein). Loss of RUVBL1 perturbs DNAAF1/IFT88 co-localization, and RUVBL1 orthologs show asymmetric left-sided distribution at the embryonic node and zebrafish Kupffer's vesicle. Conditional Ruvbl1 deletion in tubular epithelial cells causes renal failure with fewer ciliated cells; deletion in motile-ciliated cells causes hydrocephalus. Cilia of Ruvbl1-negative cells lack crucial ciliary proteins, consistent with cytoplasmic pre-assembly of ciliary complexes. Co-immunoprecipitation (RUVBL1-DNAAF1-IFT88), conditional mouse knockout (renal epithelium, motile cilia), immunofluorescence, zebrafish asymmetry assay Human molecular genetics / Experimental & molecular medicine High 29228333 29959317
2019 The cryo-EM structure of the human R2TP co-chaperone complex (RUVBL1-RUVBL2-RPAP3-PIH1D1) reveals that binding of PIH1D1 to the DII domain of RUVBL2 induces conformational rearrangements that destabilize an N-terminal segment of RUVBL2 acting as a gatekeeper to nucleotide exchange, thereby regulating RUVBL2 ATPase activity. This DII-mediated nucleotide access mechanism is proposed to be general. Cryo-EM structure determination, biochemical validation of conformational changes Science advances High 31049401
2019 CB-6644 is an allosteric small-molecule inhibitor of the RUVBL1/2 ATPase complex; drug-resistant clones carry mutations in RUVBL1 or RUVBL2, confirming on-target mechanism. Inhibition of RUVBL1/2 ATPase activity leads to cancer cell death and reduced tumor growth in AML and multiple myeloma xenograft models. In vitro ATPase inhibition assay, acquired resistance mutation mapping, xenograft tumor models ACS chemical biology High 30640450
2019 RUVBL1/2 ATPase activity is necessary for maturation/dissociation of the PAQosome (large RUVBL1/2-dependent multiprotein complex) and for DNA replication; its inhibition causes S-phase arrest and replication catastrophe in cancer cells. RUVBL1/2 ATPase inhibitor treatment, PAQosome complex analysis, S-phase flow cytometry, replication assay Cell chemical biology Medium 31883965
2019 RUVBL1 promotes the RAF/MEK/ERK pathway by binding C-RAF and inhibiting phosphorylation of C-RAF at serine 259, thereby activating the pathway to promote lung cancer cell proliferation and invasion. Co-immunoprecipitation (RUVBL1-CRAF), immunoblot for pS259-CRAF, RUVBL1 knockout (cell proliferation/invasion assay), xenograft model Biochemical and biophysical research communications Medium 29545175
2020 RUVBL1-RUVBL2 complex controls assembly and composition of the gamma-tubulin ring complex (γTuRC) in human cells. RUVBL1/2 interacts with γTuRC subcomplexes but is absent from fully assembled γTuRC. Purified, RUVBL1/2-assembled recombinant γTuRC has microtubule nucleation activity and resembles native γTuRC by cryo-EM (~4.0 Å). siRNA knockdown (γTuRC assembly), heterologous coexpression reconstitution, cryo-EM structure (~4.0 Å), co-immunoprecipitation, microtubule nucleation assay Science advances High 33355144
2020 Cryo-EM reveals that DHX34, an RNA helicase regulating NMD initiation, directly interacts with RUVBL1-RUVBL2 in vitro and in cells. DHX34 binding induces extensive conformational changes in N-termini of every RUVBL2 subunit, stabilizing a conformation that cannot bind nucleotide, downregulating RUVBL2 ATP hydrolysis. ATPase-deficient mutants show DHX34 acts exclusively on RUVBL2 subunits. Cryo-EM structure, in vitro binding assay, co-immunoprecipitation, ATPase-deficient mutant analysis eLife High 33205750
2020 Both excess and depletion of RUVBL1 impede DNA replication through transcription-dependent mechanisms. RUVBL1 overexpression increases c-Myc-dependent RNAPII pause release and transcription; RUVBL1 depletion increases Rpb1 ubiquitination and reduces RNAP II mobility, causing stalled RNAPII. Both states result in replication-transcription interference. siRNA knockdown, RUVBL1 overexpression, pSer2 CTD immunoblot, Rpb1 ubiquitination assay, FRAP, replication fork rate measurement The international journal of biochemistry & cell biology Medium 32846207
2021 NOPCHAP1 (C12ORF45) acts as a PAQosome cofactor that bridges NOP58 to the PAQosome by making direct physical interactions with the CC-NOP domain of NOP58 and domain II of RUVBL1/2 AAA+ ATPases. NOPCHAP1 interaction with RUVBL1/2 is disrupted upon ATP binding, suggesting a nucleotide-regulated client-loading mechanism for C/D snoRNP biogenesis. Co-immunoprecipitation, in vitro direct binding assay, ATP competition assay, NOP58 KO cell analysis, proteomic pulldown Nucleic acids research High 33367824
2021 RUVBL1 interacts with SMCHD1 and is present at D4Z4 chromatin; loss of RUVBL1 further derepresses DUX4 in FSHD myocytes, placing RUVBL1 in a SMCHD1-dependent chromatin repression pathway at the D4Z4 locus. Quantitative proteomics (SMCHD1 interactome), ChIP, siRNA knockdown, DUX4 derepression assay Scientific reports Medium 34880314
2022 Rvb1/Rvb2 are enriched at promoters and mRNAs of alternative glucose metabolism genes during starvation. Engineered Rvb1/Rvb2 tethering to mRNAs is sufficient to sequester those mRNAs into mRNP granules, repress their translation, and drive further transcriptional upregulation of target genes, demonstrating coupling of transcription, mRNA localization, and translation. ChIP, RNA immunoprecipitation, mRNA tethering (engineered binding), stress granule imaging, ribosome profiling, genetic depletion eLife Medium 36107469
2022 LINC00839 (lncRNA) recruits RUVBL1 to the Tip60 complex and increases its acetyltransferase activity; the complex is guided to the NRF1 promoter and promotes H4K5 and H4K8 acetylation to upregulate NRF1 expression. Co-immunoprecipitation (LINC00839-RUVBL1-TIP60), ChIP (H4K5Ac, H4K8Ac at NRF1 promoter), siRNA/shRNA knockdown, reporter assay EMBO reports Medium 35876654
2022 RUVBL1/2 inhibition significantly reduces histone H3K4me3 at the Nos2 and Il6 promoters and diminishes NF-κB recruitment to corresponding enhancers, demonstrating that RUVBL1/2 regulate macrophage pro-inflammatory gene expression through epigenetic H3K4 trimethylation. siRNA knockdown, RUVBL1/2 pharmacological inhibition, ChIP (H3K4me3, NF-κB), transcriptome analysis, functional NO production assay Frontiers in immunology Medium 34276666
2022 DPCD directly interacts with RUVBL1 and RUVBL2 in vitro and in cells, predominantly via DII domains of RUVBL1/2. DPCD binding disrupts the dodecameric state of the RUVBL1/2 complex. Co-immunoprecipitation (in vivo), in vitro direct binding assay, SAXS, structural mass spectrometry, electron microscopy Journal of molecular biology High 35901867
2023 GART methylates RUVBL1 at K7, enhancing RUVBL1 protein stability. This stabilization aberrantly activates the Wnt/β-catenin signaling pathway to induce tumor stemness in colorectal cancer. In vitro methyltransferase assay (GART on RUVBL1 K7), site-directed mutagenesis (E948 active site), co-immunoprecipitation, protein stability assay, β-catenin pathway reporter Advanced science Medium 37439412
2023 RUVBL1 controls MYC chromatin binding and modulates MYC-driven EEF1A1 expression and protein synthesis. A high-density CRISPR gene body scan identified the MYC-interacting residue(s) in RUVBL1 critical for this function. CRISPR screen (gene body scan), ChIP (MYC binding), RUVBL1 suppression (shRNA/CRISPR), protein synthesis assay Advanced science Medium 37075745
2024 DTL ubiquitinates RUVBL1 and facilitates RUVBL1 binding to RUVBL2 and β-catenin. Ubiquitinated RUVBL1 promotes transcriptional regulation of NHEJ repair pathway genes via the RUVBL1/2–β-catenin complex, while attenuating TIP60-mediated H4K16 acetylation and HR repair, thereby enhancing radioresistance. Co-immunoprecipitation (DTL-RUVBL1, RUVBL1-RUVBL2-β-catenin), ubiquitination assay, ChIP (NHEJ gene promoters, H4K16Ac), siRNA/knockdown, in vitro and in vivo radioresistance assays Cell death & disease Medium 38609375
2024 RUVBL1 is required for MYC to establish oncogenic and immunoevasive gene expression in pancreatic ductal adenocarcinoma (PDAC). Degradation of RUVBL1 (auxin-degron system) arrests cancer but not untransformed cells and causes complete tumor regression in mice, preceded by immune cell infiltration. shRNA library screen (in vitro and in vivo), auxin-degron targeted degradation, gene expression analysis, in vivo PDAC mouse tumor model Gut High 38821858
2024 RUVBL1 promotes enzalutamide resistance in prostate cancer by localizing to the cytoplasm upon enzalutamide treatment, enhancing recruitment of CRAF to plexin A1 (PLXNA1), and activating the downstream MAPK pathway. Co-immunoprecipitation (RUVBL1-CRAF-PLXNA1), subcellular fractionation (cytoplasmic RUVBL1), siRNA/CB-6644 inhibition, xenograft model Oncogene Medium 35508542
2024 RUVBL1/2 reciprocally interact with YTHDF1 at 40S translation initiation complexes (identified by Co-IP and mass spectrometry). RUVBL1/2 loss stalls YTHDF1-driven oncogenic translation and nascent protein biosynthesis; ribosome sequencing shows impaired MAPK, RAS, and PI3K-AKT signaling translation upon RUVBL1/2 depletion. Co-immunoprecipitation, mass spectrometry, ribosome sequencing, siRNA knockdown, polysome profiling Cancer research High 38900944

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity. The EMBO journal 238 11080158
2009 RVB1/RVB2: running rings around molecular biology. Molecular cell 194 19524533
2012 Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Molecular cell 186 23142078
1998 Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proceedings of the National Academy of Sciences of the United States of America 153 9843967
2006 Crystal structure of the human AAA+ protein RuvBL1. The Journal of biological chemistry 137 17060327
2017 PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Molecular cell 131 28238654
2010 AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Science signaling 131 20371770
2008 Human Rvb1/Tip49 is required for the histone acetyltransferase activity of Tip60/NuA4 and for the downregulation of phosphorylation on H2AX after DNA damage. Molecular and cellular biology 130 18285460
1999 TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. The Journal of biological chemistry 122 10428817
1998 An eukaryotic RuvB-like protein (RUVBL1) essential for growth. The Journal of biological chemistry 117 9774387
2019 CircMYO10 promotes osteosarcoma progression by regulating miR-370-3p/RUVBL1 axis to enhance the transcriptional activity of β-catenin/LEF1 complex via effects on chromatin remodeling. Molecular cancer 114 31665067
2011 Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex. Journal of structural biology 105 21933716
1997 Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB. Biochemical and biophysical research communications 100 9196036
2017 R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nature communications 96 28561026
2003 TIP49 regulates beta-catenin-mediated neoplastic transformation and T-cell factor target gene induction via effects on chromatin remodeling. Cancer research 90 14695187
2005 Myc interacts genetically with Tip48/Reptin and Tip49/Pontin to control growth and proliferation during Drosophila development. Proceedings of the National Academy of Sciences of the United States of America 86 16087886
2014 Autoantibodies to RuvBL1 and RuvBL2: a novel systemic sclerosis-related antibody associated with diffuse cutaneous and skeletal muscle involvement. Arthritis care & research 85 24023044
2006 Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. Journal of molecular biology 85 17157868
2013 Chaperone-like activity of the AAA+ proteins Rvb1 and Rvb2 in the assembly of various complexes. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 83 23530256
2008 Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex. Journal of molecular biology 77 18234224
2002 TIP49, but not TRRAP, modulates c-Myc and E2F1 dependent apoptosis. Oncogene 72 12185582
1999 A rat RuvB-like protein, TIP49a, is a germ cell-enriched novel DNA helicase. The Journal of biological chemistry 72 10336418
2019 CB-6644 Is a Selective Inhibitor of the RUVBL1/2 Complex with Anticancer Activity. ACS chemical biology 70 30640450
2020 RUVBL1-RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Current opinion in structural biology 63 33129013
2019 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell chemical biology 60 31883965
2010 Rvb1-Rvb2: essential ATP-dependent helicases for critical complexes. Biochemistry and cell biology = Biochimie et biologie cellulaire 60 20130677
2003 The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. Cell motility and the cytoskeleton 60 14506706
2001 Purification, cloning, and characterization of a profibrinolytic plasminogen-binding protein, TIP49a. The Journal of biological chemistry 57 11027681
2009 Cooperative action of TIP48 and TIP49 in H2A.Z exchange catalyzed by acetylation of nucleosomal H2A. Nucleic acids research 54 19696079
2012 Conformational transitions regulate the exposure of a DNA-binding domain in the RuvBL1-RuvBL2 complex. Nucleic acids research 51 23002137
2018 Presence of anti-eukaryotic initiation factor-2B, anti-RuvBL1/2 and anti-synthetase antibodies in patients with anti-nuclear antibody negative systemic sclerosis. Rheumatology (Oxford, England) 49 29294089
2020 Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Science advances 47 33355144
2015 RuvbL1 and RuvbL2 enhance aggresome formation and disaggregate amyloid fibrils. The EMBO journal 47 26303906
2022 LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1. EMBO reports 44 35876654
2017 Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones. Cell reports 44 28591576
2014 Structure of Yin Yang 1 oligomers that cooperate with RuvBL1-RuvBL2 ATPases. The Journal of biological chemistry 43 24990942
2019 Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM. Science advances 42 31049401
1998 TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human. Biochemical and biophysical research communications 42 9588198
2010 Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes. The Biochemical journal 39 20412048
2011 Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex. Cancer science 38 21951644
2010 Comparison of the multiple oligomeric structures observed for the Rvb1 and Rvb2 proteins. Biochemistry and cell biology = Biochimie et biologie cellulaire 38 20130681
2008 RAD51 foci formation in response to DNA damage is modulated by TIP49. The international journal of biochemistry & cell biology 37 18834951
2014 Abundance of the Fanconi anaemia core complex is regulated by the RuvBL1 and RuvBL2 AAA+ ATPases. Nucleic acids research 36 25428364
2011 A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis. EMBO reports 36 21617703
2012 Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2. Nucleus (Austin, Tex.) 35 22540023
2018 DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport. Human molecular genetics 30 29228333
2012 Large-scale conformational flexibility determines the properties of AAA+ TIP49 ATPases. Structure (London, England : 1993) 29 22748767
2015 Chromosome Missegregation Associated with RUVBL1 Deficiency. PloS one 28 26201077
2023 GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 27 37439412
2003 Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a. Molecular cancer 26 14675489
2023 Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 25 37075745
2022 RUVBL1 promotes enzalutamide resistance of prostate tumors through the PLXNA1-CRAF-MAPK pathway. Oncogene 25 35508542
2020 Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells. Science advances 25 32789167
2015 A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Molecular and cellular biology 25 26711270
1999 Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins. Biochimica et biophysica acta 25 10524211
2018 Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus. Experimental & molecular medicine 23 29959317
2014 RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. International journal of oncology 23 24728183
2019 Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. Viruses 22 31018511
2019 The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis. The Plant journal : for cell and molecular biology 21 30834599
2017 Pih1p-Tah1p Puts a Lid on Hexameric AAA+ ATPases Rvb1/2p. Structure (London, England : 1993) 21 28919439
2016 Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 21 27722820
1999 A notable example of an evolutionary conserved gene: studies on a putative DNA helicase TIP49. DNA sequence : the journal of DNA sequencing and mapping 21 10565543
2022 Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling. Theranostics 20 36438486
2021 NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis. Nucleic acids research 20 33367824
2017 RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer. Biochimica et biophysica acta. General subjects 20 28341484
2019 Liver haploinsufficiency of RuvBL1 causes hepatic insulin resistance and enhances hepatocellular carcinoma progression. International journal of cancer 18 31721195
2015 Yeast rvb1 and rvb2 proteins oligomerize as a conformationally variable dodecamer with low frequency. Journal of molecular biology 18 25636407
2024 RUVBL1 ubiquitination by DTL promotes RUVBL1/2-β-catenin-mediated transcriptional regulation of NHEJ pathway and enhances radiation resistance in breast cancer. Cell death & disease 17 38609375
2018 RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis. Biochemical and biophysical research communications 17 29545175
2017 Suppression of Type I Interferon Signaling by E1A via RuvBL1/Pontin. Journal of virology 16 28122980
2024 Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2. Gut 15 38821858
2024 RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis. Cancer research 14 38900944
2020 Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. eLife 14 33205750
2023 Combined CRISPRi and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression. American journal of human genetics 13 37541187
2021 RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation. Frontiers in immunology 13 34276666
2020 RUVBL1 is an amplified epigenetic factor promoting proliferation and inhibiting differentiation program in head and neck squamous cancers. Oral oncology 13 32745900
2018 Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila. Cell death discovery 13 30062057
2016 C-FLIPL Modulated Wnt/β-Catenin Activation via Association with TIP49 Protein. The Journal of biological chemistry 13 28028178
2000 Expression of Xenopus homologs of the beta-catenin binding protein pontin52. Mechanisms of development 13 10842076
2024 The Lyn/RUVBL1 Complex Promotes Colorectal Cancer Liver Metastasis by Regulating Arachidonic Acid Metabolism Through Chromatin Remodeling. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12 39665272
2022 Discovery of small-molecule inhibitors of RUVBL1/2 ATPase. Bioorganic & medicinal chemistry 12 35364523
2022 Involvement of RUVBL1 in WNT/β-Catenin Signaling in Oral Squamous Cell Carcinoma. Disease markers 12 35493294
2016 Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components. Molecular & cellular proteomics : MCP 11 26831523
2023 RUVBL1-modulated chromatin remodeling alters the transcriptional activity of oncogenic CTNNB1 in uveal melanoma. Cell death discovery 9 37076452
2023 EIF3D promotes resistance to 5-fluorouracil in colorectal cancer through upregulating RUVBL1. Journal of clinical laboratory analysis 8 36592991
2021 RuvBL1 Maintains Resistance to TRAIL-Induced Apoptosis by Suppressing c-Jun/AP-1 Activity in Non-Small Cell Lung Cancer. Frontiers in oncology 8 34164343
2016 The Relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in Benign and Malignant Human Lymphoid Tissues. Biochemistry and biophysics reports 8 27066592
2023 Downregulation of AHNAK2 inhibits cell cycle of lung adenocarcinoma cells by interacting with RUVBL1. Thoracic cancer 7 37349884
2022 Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. Journal of molecular biology 7 35901867
2020 Deregulated levels of RUVBL1 induce transcription-dependent replication stress. The international journal of biochemistry & cell biology 7 32846207
2008 Cloning, expression, purification, crystallization and preliminary X-ray analysis of the human RuvBL1-RuvBL2 complex. Acta crystallographica. Section F, Structural biology and crystallization communications 7 18765919
2007 Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues. FEMS yeast research 7 17302941
2022 Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation. eLife 6 36107469
2017 Human DNA helicase, RuvBL1 and its Chlamydomonas homologue, CrRuvBL1 plays an important role in ciliogenesis. Cytoskeleton (Hoboken, N.J.) 6 28574207
2023 IRX2 regulates endometrial carcinoma oncogenesis by transcriptional repressing RUVBL1. Experimental cell research 5 38042247
2024 Molecular Signatures of CB-6644 Inhibition of the RUVBL1/2 Complex in Multiple Myeloma. International journal of molecular sciences 4 39201707
2024 Regulation of the β‑catenin/LEF‑1 pathway by the siRNA knockdown of RUVBL1 expression inhibits breast cancer cell proliferation, migration and invasion. Oncology reports 4 39670302
2021 Leishmania major RUVBL1 has a hexameric conformation in solution and, in the presence of RUVBL2, forms a heterodimer with ATPase activity. Archives of biochemistry and biophysics 4 33775623
2021 A proteomics study identifying interactors of the FSHD2 gene product SMCHD1 reveals RUVBL1-dependent DUX4 repression. Scientific reports 4 34880314
2000 Chromosome mapping and expression of human tip49 family genes. DNA sequence : the journal of DNA sequencing and mapping 4 10902922

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