Affinage

RPL23

Large ribosomal subunit protein uL14 · UniProt P62829

Length
140 aa
Mass
14.9 kDa
Annotated
2026-06-10
91 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPL23/uL14 is a large-subunit ribosomal protein that performs dual structural and regulatory roles, positioned at the peptide exit tunnel where it both anchors co-translational factors and, when extra-ribosomal, controls the p53 pathway (PMID:15314173, PMID:12226666). Structurally it occupies the interior of the 50S/60S subunit, with a flexible loop that rigidifies upon rRNA binding to form part of the exit tunnel wall, spanning the region between the peptidyl transferase center and the peptide exit site (PMID:12766408, PMID:9079702). At the exit tunnel it serves as the essential docking platform for nascent-chain-processing factors—Trigger Factor, SRP, and the nascent polypeptide-associated complex—which contact L23 and compete for this surface (PMID:12226666, PMID:12756233, PMID:16316984). Its C-terminal tail forms an interface with the anti-association factor eIF6 at the subunit interface, an interaction required for cancer cell proliferation, while nuclear chaperoning of free RPL23 by BCCIPβ stabilizes the protein and supports 60S biogenesis (PMID:36651285, PMID:25150171, PMID:27913624). Beyond the ribosome, RPL23 is a sensor of ribosomal/nucleolar stress: it dissociates and binds the central acidic domain of MDM2/HDM2 to block MDM2-mediated p53 ubiquitination and degradation, triggering p53-dependent G1 arrest (PMID:15314173, PMID:15314174). This RPL23–MDM2–p53 axis is engaged downstream of oncogenic RAS signaling, which upregulates RPL23 via MEK/PI3K/mTOR (PMID:27402081), and is set by GRWD1/EDD-mediated ubiquitin-proteasomal turnover of RPL23 (PMID:29991511). RPL23 additionally restrains the Miz1/c-Myc/nucleophosmin circuit by retaining nucleophosmin in the nucleolus to suppress Miz1-driven induction of the CDK inhibitors p15(Ink4b) and p21(Cip1), forming a Myc-responsive feedback loop coupling growth to cell-cycle control (PMID:19160485, PMID:28539603). Loss of RPL23 also downregulates antigen processing and presentation, impairing CD8+ T cell recognition of tumor cells (PMID:40918646).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1984 Medium

    Establishing where L23 sits in the ribosome was the first mechanistic question; affinity labeling placed it within the A-site/peptidyl transferase domain of the 50S subunit with extensive rRNA contacts.

    Evidence Puromycin affinity labeling and chemical/ribonuclease RNA footprinting in E. coli

    PMID:6392564

    Open questions at the time
    • Resolution insufficient to define exit-tunnel role
    • rRNA binding site mapped only at the fragment level
  2. 1997 Medium

    Refining its position resolved that L23 spans the interior tunnel linking the transferase center to the peptide exit site, foreshadowing its role at the nascent-chain emergence point.

    Evidence Immune electron microscopy of reconstituted 50S subunits with DNP-modified L23, plus reconstitution functional assays

    PMID:1960726 PMID:2191716 PMID:6170935 PMID:9079702

    Open questions at the time
    • Two localization sites observed without single high-resolution placement
    • Functional consequence at the exit tunnel not yet tested
  3. 2002 High

    The exit-tunnel positioning was given function: L23 is the essential docking site for Trigger Factor, coupling the protein to co-translational chaperone delivery.

    Evidence Crosslinking, in vitro binding, site-directed mutagenesis and genetic complementation in E. coli

    PMID:12226666

    Open questions at the time
    • Whether the same surface accommodates other factors not yet defined here
    • Eukaryotic generalization untested in this study
  4. 2003 High

    L23 was shown to be a shared, competed-for hub for multiple targeting factors, establishing it as a regulatory node for nascent-chain fate at the exit site.

    Evidence Site-specific UV crosslinking and competitive in vitro binding assays mapping SRP/Ffh and Trigger Factor to L23; NMR solution structure of L23

    PMID:12702815 PMID:12756233 PMID:12766408

    Open questions at the time
    • Competition outcome on actively translating ribosomes not fully resolved
    • Structural basis of factor discrimination not defined
  5. 2004 High

    The discovery that L23 binds the central acidic domain of MDM2/HDM2 independent of the ribosome answered how a structural ribosomal protein signals stress, revealing an extra-ribosomal p53-stabilizing function.

    Evidence Immunoaffinity purification, domain-mapped reciprocal co-IP, p53 ubiquitination assays, siRNA knockdown and flow cytometry in human cells

    PMID:15314173 PMID:15314174

    Open questions at the time
    • Trigger that releases L23 from the ribosome not mechanistically defined
    • Stoichiometry within the L11/L23/MDM2 ternary complex unresolved
  6. 2004 Medium

    An additional cancer phenotype was linked to RPL23 — multidrug resistance — though through correlative anti-apoptotic and detoxification changes rather than a defined molecular interaction.

    Evidence Stable overexpression in gastric cancer cells, drug sensitivity assays, Western blot, enzyme activity assays

    PMID:15149863

    Open questions at the time
    • No direct protein interaction established for the resistance phenotype
    • Causal link to Bcl-2/GST changes correlative
  7. 2008 High

    RPL23 was tied to a second growth-control circuit: it suppresses Miz1 transactivation by sequestering nucleophosmin in the nucleolus, and is itself a Myc target, forming a translation-to-cell-cycle feedback loop.

    Evidence Co-IP, nucleolar retention assays, Miz1 reporter assays and knockdown

    PMID:19160485

    Open questions at the time
    • How nucleophosmin partitioning is dynamically regulated unclear
    • Crosstalk with the MDM2/p53 axis not integrated
  8. 2014 High

    The biogenesis side of RPL23 was clarified: BCCIPβ acts as a nuclear chaperone that stabilizes free RPL23 and forms a ternary complex with the 60S factor eIF6.

    Evidence Reciprocal co-IP, siRNA depletion, subcellular fractionation and overexpression in human cells; yeast Bcp1 genetics

    PMID:25150171 PMID:27913624

    Open questions at the time
    • Whether chaperoning competes with the extra-ribosomal MDM2 pool unknown
    • Structural basis of the BCCIPβ C-terminal requirement undefined at this stage
  9. 2016 High

    The RPL23–MDM2–p53 axis was placed in an oncogenic signaling context, showing RAS drives RPL23 upregulation through MEK/PI3K/mTOR to activate p53 as tumor suppression.

    Evidence Mouse genetics (MDM2 C305F x Hras G12V), pathway inhibitors, siRNA and co-IP

    PMID:27402081

    Open questions at the time
    • Direct transcriptional/translational mechanism of RPL23 induction not fully resolved
    • Relative contribution versus other ribosomal stress sensors unquantified
  10. 2018 High

    RPL23 abundance was shown to be set by regulated proteolysis, with GRWD1 and the E3 ligase EDD/UBR5 driving its ubiquitin-proteasomal degradation to dampen p53.

    Evidence Co-IP/proteomics, ubiquitylation assay, MG132 rescue and siRNA in human cells

    PMID:29991511

    Open questions at the time
    • Signals controlling GRWD1/EDD activity toward RPL23 unknown
    • Selectivity for free versus ribosome-bound RPL23 unaddressed
  11. 2019 Medium

    The RPL23–MDM2–p53 axis was extended to a disease context beyond cancer, implicating it in dopaminergic neuron death downstream of FBXO7 loss.

    Evidence Conditional knockout mouse model, immunohistochemistry, transcriptional profiling

    PMID:31144295

    Open questions at the time
    • Direct RPL23-MDM2 binding not confirmed in this study
    • Pathway placement relies on prior mechanistic work
  12. 2021 Medium

    An RNA-regulatory function emerged: RPL23 binds the MMP9 3'UTR to promote its expression and drive HCC invasion and metastasis.

    Evidence RNA immunoprecipitation, siRNA knockdown, invasion/migration and in vivo metastasis assays

    PMID:34926291

    Open questions at the time
    • Binding shown by a single method (RIP) without reciprocal validation
    • Mechanism of mRNA stabilization/translation not defined
  13. 2023 High

    The RPL23–eIF6 interface was mapped to the uL14 C-terminal tail, defining eight key residues and linking interface disruption to suppression of cancer cell proliferation.

    Evidence Recombinant interaction assays, HDX-MS, mutagenesis and proliferation assays

    PMID:36651285

    Open questions at the time
    • In vivo consequence of interface disruption untested
    • Connection to SDS disease mechanism characterized only via eIF6 mutants
  14. 2024 Medium

    Nucleolar retention of RPL23 was shown to be actively regulated, via U2AF2-SNORA68, with nucleolar RPL23 upregulating c-Myc to promote breast cancer stemness.

    Evidence RNA pull-down, RIP, fractionation, co-IP and xenograft

    PMID:38594783

    Open questions at the time
    • Molecular mechanism of c-Myc upregulation by nucleolar RPL23 unclear
    • Relationship to the Miz1/nucleophosmin circuit not integrated
  15. 2025 Medium

    A new cell-extrinsic role was uncovered: RPL23 loss downregulates antigen processing/presentation and impairs CD8+ T cell killing, linking ribosomal protein levels to tumor immune escape.

    Evidence siRNA knockdown, proteomics/peptidome analysis, T cell co-culture cytotoxicity assay in melanoma cells

    PMID:40918646

    Open questions at the time
    • Mechanism linking RPL23 to APP gene expression undefined
    • Whether effect depends on global translation or a specific pathway unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RPL23 is partitioned between its ribosomal, nuclear-chaperoned, and extra-ribosomal (MDM2/Miz1/RNA-binding) pools, and what dictates which function dominates under a given stress, remains unresolved.
  • No unified model coupling exit-tunnel occupancy to stress release
  • Quantitative control of free versus ribosome-bound RPL23 unmeasured
  • Eukaryotic structural basis for exit-tunnel chaperone docking not directly established in the corpus

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 4 GO:0003723 RNA binding 3 GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005730 nucleolus 4 GO:0005840 ribosome 4 GO:0005634 nucleus 2 GO:0005654 nucleoplasm 2
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-392499 Metabolism of proteins 5 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-168256 Immune System 1
Partners
BCCIPEEF2EIF6GRWD1MDM2NPM1U2AF2UBR5
Complex memberships
60S large ribosomal subunitL11/L23/MDM2 ternary complexRPL23-BCCIPβ-eIF6 ternary complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 RPL23 interacts with MDM2 (via immunoaffinity purification), forming a complex independent of the 80S ribosome and polysome. This interaction is enhanced by actinomycin D (ribosomal perturbation) but not gamma-irradiation. Ectopic L23 expression reduces MDM2-mediated p53 ubiquitination and induces p53 activity and G1 arrest in p53-proficient cells; siRNA knockdown of L23 abolishes this activation. Immunoaffinity purification, co-IP, siRNA knockdown, ubiquitination assay, flow cytometry Molecular and cellular biology High 15314173
2004 RPL23 (L23) interacts with HDM2 through the central acidic domain of HDM2 and an N-terminal domain of L23. L23 and L11 can simultaneously yet distinctly bind HDM2, forming a ternary complex. Overexpressed L23 inhibits HDM2-induced p53 polyubiquitination and degradation and causes p53-dependent cell cycle arrest. Knockdown of L23 triggers nucleolar stress, translocation of B23 from nucleolus to nucleoplasm, and stabilization/activation of p53. Co-IP, domain-mapping experiments, p53 ubiquitination assay, siRNA knockdown, flow cytometry Molecular and cellular biology High 15314174
2002 In E. coli, L23 (bacterial ortholog of RPL23/uL14) is located at the ribosomal peptide exit tunnel and serves as an essential docking site for the chaperone Trigger Factor. Mutation of an exposed glutamate in L23 prevents Trigger Factor from interacting with ribosomes and nascent chains, causes protein aggregation, and causes conditional lethality. Purified L23 interacts specifically with Trigger Factor in vitro. Crosslinking, in vitro binding assay, mutagenesis, genetic complementation Nature High 12226666
2003 The signal recognition particle (SRP) component Ffh crosslinks to ribosomal protein L23 at the peptide exit of the E. coli 50S subunit. Two positions in the N domain of Ffh crosslink predominantly to L23. Binding of a nascent signal peptide induces a slightly different arrangement of SRP on L23. Site-specific UV-induced crosslinking (p-azidophenacyl bromide), biochemical mapping RNA High 12702815
2003 Both Trigger Factor (TF) and SRP interact with the signal anchor sequence of nascent inner membrane proteins and contact L23 and L29 at the ribosomal exit site. TF and SRP compete for binding to L23 on non-translating ribosomes, with SRP having a competitive advantage. Photocrosslinking of nascent chains, in vitro binding assay with purified components The Journal of cell biology High 12756233
2005 The nascent polypeptide-associated complex (NAC) contacts L23 (ribosomal protein family member) at the ribosomal exit site via a conserved RRK(X)nKK ribosome-binding motif in the β-subunit of NAC. UV-crosslinking of the motif specifically crosslinks to L23 at the exit site. Mutations of L23 reduce NAC ribosome binding in vivo and in vitro without affecting other ribosome-associated factors (Ssb, Zuotin). UV-activatable crosslinking, mutagenesis of L23, in vivo and in vitro binding assays The Journal of biological chemistry High 16316984
2008 RPL23 functions as a negative regulator of Miz1-dependent transactivation by retaining nucleophosmin (an essential co-activator of Miz1) in the nucleolus, thereby preventing Miz1-induced expression of cell-cycle inhibitors p15(Ink4b) and p21(Cip1). RPL23 is encoded by a direct Myc target gene, forming a feedback circuit linking translation/cell growth with Miz1-dependent cell-cycle arrest. Co-IP, nucleolar retention assay, reporter assays for Miz1 transactivation, knockdown experiments Nature cell biology High 19160485
2003 NMR structure of ribosomal protein L23 from Thermus thermophilus determined in solution. Uncomplexed L23 has a well-ordered core (four anti-parallel beta-strands and three alpha-helices in a beta-alpha-beta-alpha-beta-beta-alpha topology) with a large flexible loop between strands 3 and 4. In RNA-complexed crystal structures, this flexible loop becomes rigid and forms part of the ribosomal exit tunnel wall through interactions with rRNA. NMR structure determination, comparison with crystal structures Journal of biomolecular NMR High 12766408
2016 RPL23 is induced downstream of oncogenic RAS signaling (requiring both MEK and PI3K pathways and mTOR function), and the resulting elevated RPL23 binds MDM2 (including the MDM2(C305F) mutant that is deficient for RPL11 binding) to activate p53. Downregulation of RPL23 abolishes RAS-induced p53 activation, establishing a RPL23-MDM2-p53 pathway specific to oncogenic RAS-induced tumor suppression. Mouse genetic crosses (MDM2(C305F) mutant x Hras(G12V) transgene), siRNA knockdown, pathway inhibitor experiments (MEK, PI3K, mTOR inhibitors), co-IP Cancer research High 27402081
2016 Bcp1 (yeast ortholog of BCCIP) acts as the nuclear chaperone of Rpl23 in Saccharomyces cerevisiae. Bcp1 dissociates Rpl23 from karyopherins and then associates with Rpl23. Loss of Bcp1 causes instability of Rpl23 and deficiency of 60S ribosomal subunits. Co-IP, genetic deletion, ribosome profiling/sedimentation, in vivo interaction assays The Journal of biological chemistry High 27913624
2014 The beta-isoform of BCCIP (BCCIPβ), but not BCCIPα, forms a ternary complex with RPL23/uL14 and the pre-60S trans-acting factor eIF6. Complex formation requires an intact C-terminal domain of BCCIPβ. Depletion of BCCIPβ reduces the pool of free RPL23 and decreases eIF6 levels in nucleoli. Overexpression of BCCIPβ leads to nucleoplasmic accumulation of extra-ribosomal RPL23 and stabilizes overexpressed RPL23, indicating BCCIPβ is a nuclear chaperone for RPL23. Co-IP (reciprocal), siRNA depletion, subcellular fractionation, overexpression assays FEBS letters High 25150171
2018 GRWD1 interacts with RPL23 and, together with the E3 ubiquitin ligase EDD/UBR5, promotes RPL23 ubiquitylation and proteasomal degradation. Overexpression of GRWD1 decreases RPL23 protein levels and stability (reversed by MG132); siRNA knockdown of GRWD1 upregulates RPL23. This GRWD1-induced RPL23 proteolysis downregulates p53. Co-IP/proteomics, siRNA knockdown, proteasome inhibitor (MG132) rescue, ubiquitylation assay Journal of cell science High 29991511
2023 The C-terminal tail of uL14 (RPL23) is essential for its physical interaction with eIF6 at the 60S ribosomal subunit interface, involving eight key residues. Disease-associated SDS mutations in eIF6 cause conformational changes that weaken the eIF6-uL14 interface. HDX-MS revealed dynamic configurational rearrangements in eIF6 upon binding to uL14 and an allosteric interface regulated by the eIF6 C-tail. Disrupting this interface markedly limits cancer cell proliferation. Recombinant protein interaction assays, hydrogen-deuterium exchange mass spectrometry (HDX-MS), mutagenesis, cell proliferation assay Nucleic acids research High 36651285
2019 Loss of FBXO7 in midbrain dopamine neurons leads to increased expression of RPL23, which inhibits MDM2 as a ribosomal stress sensor, activating a p53 transcriptional signature biased toward pro-apoptotic genes. This establishes the RPL23-MDM2-p53 axis as a mechanism underlying dopaminergic cell death in a Parkinson's disease mouse model. Conditional knockout mouse model, immunohistochemistry, transcriptional profiling The Journal of pathology Medium 31144295
2017 RPL23 knockdown in MDS cells led to increased apoptosis, G1-S arrest, upregulation of Miz-1 with transactivation of p15Ink4b and p21Cip1, and downregulation of c-Myc. Cells from higher-risk MDS patients showed elevated RPL23 and c-Myc with decreased Miz-1. This establishes an RPL23/Miz-1/c-Myc regulatory circuit where elevated RPL23 suppresses Miz-1-dependent CDK inhibitor induction. siRNA knockdown, gene microarray, flow cytometry, Western blot Scientific reports Medium 28539603
2004 Overexpression of RPL23 in gastric cancer cells enhanced resistance to multiple drugs (vincristine, adriamycin, 5-fluorouracil, cisplatin) by protecting cells from vincristine-induced apoptosis. RPL23 overexpression significantly increased Bcl-2 expression and Bcl-2/Bax ratio, and also enhanced glutathione S-transferase (GST) activity and intracellular glutathione content. Stable overexpression, drug sensitivity assays, Western blot, enzyme activity assays Experimental cell research Medium 15149863
2020 Triptolide (TP) interrupts rRNA synthesis by inhibiting RNA Pol I and UBF transcriptional activation, inducing nucleolar disintegration. This ribosomal stress increases binding of RPL23 to MDM2, releasing and stabilizing p53, which then induces apoptosis and cell cycle arrest. Co-IP, Western blot, RNA Pol I activity assay, immunofluorescence, in vivo xenograft Oncology reports Medium 32236588
1984 E. coli ribosomal protein L23 is the primary ribosomal protein crosslinked to affinity-labeled puromycin, placing it within the A-site domain of the peptidyl transferase centre on the 50S subunit. The RNA binding site of L23 consists of two main fragments of 25 and 105 nucleotides that strongly interact. Chemical probing showed altered reactivity of many nucleotides upon L23 binding, indicating large-scale RNA-protein contacts at the extremities of helices and at bulged nucleotides. Affinity labeling with puromycin, RNA footprinting with chemical reagents and ribonucleases, RNA sequencing Journal of molecular biology Medium 6392564
1990 Puromycin-labeled L23 reconstituted into E. coli 50S subunits retains virtually all peptidyl transferase activity but only 50–60% of mRNA-dependent tRNA binding stimulation activity. This indicates L23 is positioned close to (but not directly at) the peptidyl transferase center, and is close enough to interfere with tRNA binding when modified. 50S ribosome reconstitution with modified L23, peptidyl transferase assay, tRNA binding assay Biochemistry Medium 2191716
1997 Electron microscopy localized DNP-modified L23 to two sites on the E. coli 50S subunit with nearly equal frequency: one beside the central protuberance near the peptidyltransferase center, and a second at the base of the subunit near the peptide exit site. This indicates L23 spans the interior tunnel linking the transferase and exit sites. Reconstitution of 50S with DNP-L23, immune electron microscopy The Journal of biological chemistry Medium 9079702
1991 Chemical and ribonuclease footprinting of E. coli 23S rRNA revealed that L23 binding is confined to a tertiary structural motif involving a single terminal loop structure and part of an adjacent internal loop at the centre of domain III. L23 is essential for ribosomal assembly and influences secondary binding protein assembly and function of the RNA region. Chemical footprinting, ribonuclease protection assays Journal of molecular biology Medium 1960726
2021 RPL23 directly associates with the 3'UTR of MMP9 mRNA and positively regulates MMP9 expression, promoting HCC cell invasion and metastasis. RPL23 depletion inhibited HCC cell proliferation, migration, invasion, and distant metastasis. RNA immunoprecipitation (RIP), siRNA knockdown, invasion/migration assays, in vivo metastasis model Frontiers in oncology Medium 34926291
2024 U2AF2-SNORA68 promotes retention of RPL23 in the nucleolus by binding U2AF2. Nucleolar RPL23 subsequently upregulates c-Myc expression. Elevated SNORA68 leads to increased nucleolar RPL23, and this pathway promotes triple-negative breast cancer stemness. RNA pull-down, RIP, cell fractionation, co-IP, xenograft model Breast cancer research Medium 38594783
1981 RNA-protein crosslinking in E. coli ribosomes identified L23 as crosslinked to positions 137-141 in the 23S RNA sequence, localizing the RNA contact site of L23 within 23S rRNA. 2-iminothiolane crosslinking followed by UV irradiation, RNA sequencing of crosslinked fragments Nucleic acids research Medium 6170935
2025 In extracto cryo-EM at ~2.2 Å resolution showed that elongation factor eEF2•GDP is stabilized on hibernating (non-translating) ribosomes through interactions with the sarcin-ricin loop and protein uL14 (RPL23). eEF2 was found bound to >95% of hibernating ribosomes and unexpectedly also to isolated 60S subunits. In extracto cryo-EM, high-resolution 2D template matching bioRxivpreprint Medium bio_10.1101_2025.11.25.690450
2025 Loss of uL14 (RPL23) significantly downregulates antigen processing and presentation (APP) components in melanoma cells, alters the peptide pool available for MHC/HLA presentation (altered charge, anchor residues, lower predicted HLA binding), and reduces the ability of CD8+ T cells to recognize and kill melanoma cells in co-culture. siRNA knockdown, proteomics/peptide analysis, co-culture cytotoxicity assay NAR cancer Medium 40918646
2025 High-speed atomic force microscopy visualized Trigger Factor (TF) making dynamic stable and transient contacts with ribosomal proteins uL23 and bL17 specifically in ribosome-nascent chain complexes (not on non-translating ribosomes). TF exhibited transitions between extended and compact conformations during this interaction. High-speed atomic force microscopy, molecular dynamics simulations bioRxivpreprint Medium bio_10.1101_2025.05.16.654432

Source papers

Stage 0 corpus · 91 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition. Molecular and cellular biology 425 15314173
2004 Inhibition of HDM2 and activation of p53 by ribosomal protein L23. Molecular and cellular biology 308 15314174
2002 L23 protein functions as a chaperone docking site on the ribosome. Nature 284 12226666
2003 The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome. RNA (New York, N.Y.) 129 12702815
2013 Synaptic and intrinsic homeostatic mechanisms cooperate to increase L2/3 pyramidal neuron excitability during a late phase of critical period plasticity. The Journal of neuroscience : the official journal of the Society for Neuroscience 116 23678123
2003 Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome. The Journal of cell biology 116 12756233
2004 Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis. Experimental cell research 97 15149863
2008 A ribosomal protein L23-nucleophosmin circuit coordinates Mizl function with cell growth. Nature cell biology 94 19160485
2008 High frequency action potential bursts (>or= 100 Hz) in L2/3 and L5B thick tufted neurons in anaesthetized and awake rat primary somatosensory cortex. The Journal of physiology 92 18483066
2005 A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains. The Journal of biological chemistry 76 16316984
1981 The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29. Nucleic acids research 72 6170935
1976 Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39. The Journal of biological chemistry 70 1002715
1977 Isolation of eukaryotic ribosomal proteins. Purification and characterization of 60 S ribosomal subunit proteins L3, L6, L7', L8, L10, L15, L17, L18, L19, L23', L25, L27', L28, L29, L31, L32, L34, L35, L36, L36', and L37'. The Journal of biological chemistry 68 863909
2008 Purification and partial characterization of novel bacteriocin L23 produced by Lactobacillus fermentum L23. Current microbiology 53 18172715
1985 The complete primary structure of ribosomal proteins L1, L14, L15, L23, L24 and L29 from Bacillus stearothermophilus. European journal of biochemistry 50 4018095
2008 L2/3 interneuron groups defined by multiparameter analysis of axonal projection, dendritic geometry, and electrical excitability. Cerebral cortex (New York, N.Y. : 1991) 47 18802122
1995 A novel L23-related gene 40 kb downstream of the imprinted H19 gene is biallelically expressed in mid-fetal and adult human tissues. Human molecular genetics 47 8541832
1984 Structure of a protein L23-RNA complex located at the A-site domain of the ribosomal peptidyl transferase centre. Journal of molecular biology 47 6392564
1991 Attachment sites of primary binding proteins L1, L2 and L23 on 23 S ribosomal RNA of Escherichia coli. Journal of molecular biology 44 1960726
1988 Expression of the rpl23, rpl2 and rps19 genes in spinach chloroplasts. Nucleic acids research 43 3362671
2010 Presynaptic development at L4 to l2/3 excitatory synapses follows different time courses in visual and somatosensory cortex. The Journal of neuroscience : the official journal of the Society for Neuroscience 40 20861362
2000 Herpes simplex virus type 1 gene UL14: phenotype of a null mutant and identification of the encoded protein. Journal of virology 39 10590088
2017 Ribosomal protein L23 negatively regulates cellular apoptosis via the RPL23/Miz-1/c-Myc circuit in higher-risk myelodysplastic syndrome. Scientific reports 37 28539603
2007 The UL14 tegument protein of herpes simplex virus type 1 is required for efficient nuclear transport of the alpha transinducing factor VP16 and viral capsids. Journal of virology 35 18032514
1994 The evolutionarily conserved ribosomal protein L23 and the cationic urease beta-subunit of Yersinia enterocolitica O:3 belong to the immunodominant antigens in Yersinia-triggered reactive arthritis: implications for autoimmunity. Molecular medicine (Cambridge, Mass.) 34 8790600
2016 Bcp1 Is the Nuclear Chaperone of Rpl23 in Saccharomyces cerevisiae. The Journal of biological chemistry 33 27913624
2014 JNK1 controls dendritic field size in L2/3 and L5 of the motor cortex, constrains soma size, and influences fine motor coordination. Frontiers in cellular neuroscience 33 25309320
1993 A large deletion in the plastid DNA of the holoparasitic flowering plant Cuscuta reflexa concerning two ribosomal proteins (rpl2, rpl23), one transfer RNA (trnI) and an ORF 2280 homologue. Current genetics 29 8358824
2016 The Interaction between Herpes Simplex Virus 1 Tegument Proteins UL51 and UL14 and Its Role in Virion Morphogenesis. Journal of virology 28 27440890
2014 The beta-isoform of the BRCA2 and CDKN1A(p21)-interacting protein (BCCIP) stabilizes nuclear RPL23/uL14. FEBS letters 28 25150171
2021 Diversity of Receptive Fields and Sideband Inhibition with Complex Thalamocortical and Intracortical Origin in L2/3 of Mouse Primary Auditory Cortex. The Journal of neuroscience : the official journal of the Society for Neuroscience 27 33593857
2002 Herpes simplex virus type 2 UL14 gene product has heat shock protein (HSP)-like functions. Journal of cell science 27 12045222
2016 RPL23 Links Oncogenic RAS Signaling to p53-Mediated Tumor Suppression. Cancer research 26 27402081
2003 Herpes simplex virus UL14 protein blocks apoptosis. Microbiology and immunology 26 14584616
2023 KCNQ2/3 Gain-of-Function Variants and Cell Excitability: Differential Effects in CA1 versus L2/3 Pyramidal Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 25 37607817
2020 Triptolide interrupts rRNA synthesis and induces the RPL23‑MDM2‑p53 pathway to repress lung cancer cells. Oncology reports 25 32236588
2001 The UL14 protein of herpes simplex virus type 2 translocates the minor capsid protein VP26 and the DNA cleavage and packaging UL33 protein into the nucleus of coexpressing cells. The Journal of general virology 24 11161269
1990 Regulation of the chicken embryonic myosin light-chain (L23) gene: existence of a common regulatory element shared by myosin alkali light-chain genes. Molecular and cellular biology 24 2342458
2019 Loss of FBXO7 results in a Parkinson's-like dopaminergic degeneration via an RPL23-MDM2-TP53 pathway. The Journal of pathology 23 31144295
2019 The signaling proteins GPR158 and RGS7 modulate excitability of L2/3 pyramidal neurons and control A-type potassium channel in the prelimbic cortex. The Journal of biological chemistry 22 31311860
2019 Nutritional status regulates algicidal activity of Aeromonas sp. L23 against cyanobacteria and green algae. PloS one 21 30861041
2013 Co-transduction of ribosomal protein L23 enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in human gastric cancer. Oncology reports 21 23933826
2020 Muscarinic Modulation of SK2-Type K+ Channels Promotes Intrinsic Plasticity in L2/3 Pyramidal Neurons of the Mouse Primary Somatosensory Cortex. eNeuro 20 32005752
2010 Inhibition of the p53-MDM2 interaction by adenovirus delivery of ribosomal protein L23 stabilizes p53 and induces cell cycle arrest and apoptosis in gastric cancer. The journal of gene medicine 20 20020415
2000 The translational apparatus of Tortula ruralis: polysomal retention of transcripts encoding the ribosomal proteins RPS14, RPS16 and RPL23 in desiccated and rehydrated gametophytes. Journal of experimental botany 20 11053454
1999 Identification and characterization of the UL14 gene product of herpes simplex virus type 2. The Journal of general virology 20 10501497
2011 Ca L23 spectrum in amorphous and crystalline phases of calcium carbonate. The journal of physical chemistry. B 19 21861462
2010 Cytotoxic activity of acetogenins and styryl lactones isolated from Goniothalamus undulatus Ridl. root extracts against a lung cancer cell line (COR-L23). Phytomedicine : international journal of phytotherapy and phytopharmacology 18 21112751
1994 Modification by brefeldin A, bafilomycin A1 and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) of cellular accumulation and intracellular distribution of anthracyclines in the non-P-glycoprotein-mediated multidrug-resistant cell line COR-L23/R. British journal of cancer 18 7912544
2002 The herpes simplex virus type 1 US11 protein binds the coterminal UL12, UL13, and UL14 RNAs and regulates UL13 expression in vivo. Journal of virology 17 12134014
2018 GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system. Journal of cell science 16 29991511
1988 The primary structures of ribosomal proteins L16, L23 and L33 from the archaebacterium Halobacterium marismortui. FEBS letters 16 3191994
1997 Evolution of the large-subunit ribosomal RNA binding site for protein L23/25. Molecular biology and evolution 15 9159935
2022 Transcriptional changes in Plasmodium falciparum upon conditional knock down of mitochondrial ribosomal proteins RSM22 and L23. PloS one 14 36201550
2024 U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression. Breast cancer research : BCR 13 38594783
2023 Mapping thalamic innervation to individual L2/3 pyramidal neurons and modeling their 'readout' of visual input. Nature neuroscience 13 36732641
2011 Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16. Virology journal 13 21791071
2003 NMR structure of the ribosomal protein L23 from Thermus thermophilus. Journal of biomolecular NMR 13 12766408
1988 Sequence of the gene for ribosomal protein L23 from the archaebacterium Methanococcus vannielii. FEBS letters 13 3181433
2021 Ribosomal Protein L23 Drives the Metastasis of Hepatocellular Carcinoma via Upregulating MMP9. Frontiers in oncology 12 34926291
1990 Reconstitution of Escherichia coli 50S ribosomal subunits containing puromycin-modified L23: functional consequences. Biochemistry 11 2191716
2007 Antiapoptotic activity of bovine herpesvirus type-1 (BHV-1) UL14 protein. Veterinary microbiology 9 17408888
1993 Cross-linked amino acids in the protein pairs L3-L19 and L23-L29 of Bacillus stearothermophilus ribosomes after treatment with diepoxybutane. The Journal of biological chemistry 9 8444837
1976 An investigation of the binding sites of proteins S8, L23 and L24 on the ribosomal RNAs of Escherichia coli by electron microscopy. Molecular & general genetics : MGG 9 787753
2011 Screening and identification of host factors interacting with UL14 of herpes simplex virus 1. Medical microbiology and immunology 8 21512757
1991 The primary structure of rat ribosomal protein L23. Biochemical and biophysical research communications 7 1840488
2023 Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunit. Nucleic acids research 6 36651285
2023 Effector protein Hcp2a of avian pathogenic Escherichia coli interacts with the endoplasmatic reticulum associated RPL23 protein of chicken DF-1 fibroblasts. Veterinary research 6 36717947
2022 The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication. Poultry science 6 35489250
2021 Analysis of segmentation ontology reveals the similarities and differences in connectivity onto L2/3 neurons in mouse V1. Scientific reports 6 33654118
2000 Disruption of the MRP-L23 gene encoding the mitochondrial ribosomal protein L23 is lethal for Kluyveromyces lactis but not for Saccharomyces cerevisiae. Current genetics 6 10743564
1993 Nucleotide sequence of the genes encoding the L3, L4, and L23 equivalent ribosomal proteins from the archaebacterium Halobacterium halobium. Biochimica et biophysica acta 6 8241282
2025 CALB1 and RPL23 Are Essential for Maintaining Oocyte Quality and Function During Aging. Aging cell 5 39748132
2017 The uS8, uS4, eS31, and uL14 Ribosomal Protein Genes Are Dysregulated in Nasopharyngeal Carcinoma Cell Lines. BioMed research international 5 28791303
2000 Engineering of the rpl23 gene cluster to replace the plastid RNA polymerase alpha subunit with the Escherichia coli homologue. Current genetics 5 11126781
2022 Targeting RPL23 restores chemosensitivity of cisplatin-resistant ovarian carcinoma by inhibiting EMT. Cytotechnology 4 35733701
2025 Aging reduces excitatory bandwidth, alters spectral tuning curve diversity, and reduces sideband inhibition in L2/3 of primary auditory cortex. bioRxiv : the preprint server for biology 3 40236140
2002 Intercellular trafficking of herpes simplex virus type 2 UL14 deletion mutant proteins. Biochemical and biophysical research communications 3 12413948
2000 Genomic organization and chromosome location of the murine Rpl23 gene. Cytogenetics and cell genetics 3 11124519
2024 Study on the role of RPL23 gene in active immunity of termite Reticulitermes chinensis against Metarhizium anisopliae. Journal of invertebrate pathology 2 39448023
2015 Growth inhibitory effect of adenovirus-mediated tissue-targeted expression of ribosomal protein L23 on human colorectal carcinoma cells. Oncology reports 2 26044651
1997 Incorporation of dinitrophenyl protein L23 into totally reconstituted Escherichia coli 50 S ribosomal subunits and its localization at two sites by immune electron microscopy. The Journal of biological chemistry 2 9079702
1987 A chicken embryo-specific myosin light chain (L23) appears to be identical with one of brain myosin light chains. Journal of biochemistry 2 3325506
2004 Simulation of electron energy loss near-edge structure at the Al and N K edges and Al L(23) edge in cubic aluminium nitride. Ultramicroscopy 1 15013513
2026 A transient sex-biased transcriptional program shapes early postnatal L2/3 neuron development. Biology of sex differences 0 41952201
2026 GluN3A is required for coordinated postnatal development of axonal and dendritic branching patterns in mouse L2/3 callosal projection neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 0 42150879
2025 Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance. NAR cancer 0 40918646
2024 Corrigendum: Ribosomal protein L23 drives the metastasis of hepatocellular carcinoma via upregulating MMP9. Frontiers in oncology 0 38525425
2024 Multiple functions of the herpesvirus UL14 gene product in viral infection. Frontiers in microbiology 0 39507342
2024 Regulation of XOR function of reduced human L2/3 pyramidal neurons. Cognitive neurodynamics 0 39712106
1982 [Latent infection of continuous L23 cells caused by the agent of scrapie]. Voprosy virusologii 0 6818770

Missed literature

Know a paper Affinage missed for RPL23? Flag it for the maintainers and the community.

No submissions yet.