Affinage

RBM5

RNA-binding protein 5 · UniProt P52756

Length
815 aa
Mass
92.2 kDa
Annotated
2026-06-10
100 papers in source corpus 28 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBM5 is a multi-domain nuclear RNA-binding protein that functions as a sequence-specific regulator of alternative pre-mRNA splicing and, through it, controls apoptosis and cell proliferation (PMID:18851835, PMID:38537639, PMID:37790489, PMID:24332178). Transcriptome-wide, RBM5 (together with RBM10) is engaged with the U2 snRNP at intron branch sites via a conserved zinc-finger-containing peptide, and this association is required for its repression of many alternative exons (PMID:38537639, PMID:37790489). Its modular architecture underlies this activity: a cooperative RRM1–ZnF1 module sandwiches target RNA and recognizes a GG dinucleotide, with RRM2 contributing additional CU/GA-rich RNA contacts, while the OCRE domain adopts a unique β-sheet fold that binds the proline-rich tails of spliceosomal SmN/B/B' proteins to govern splice-site pairing of FAS/CD95 pre-mRNA (PMID:27894420, PMID:37454201, PMID:22839758). RBM5 acts mechanistically at the spliceosome by inhibiting the transition from prespliceosomal to mature complexes around the regulated exon and by competing with U2AF65 at weak 3' splice sites (PMID:18851835, PMID:23017209), and its G-patch domain directly stimulates the helicase activity of DHX15 (PMID:22569250). Through these activities it promotes the proapoptotic caspase-2L isoform by binding a U/C-rich element upstream of the In100 repressor (PMID:18840686), regulates FAS exon 6 inclusion (in cooperation with lncRNA MGC32805 and antagonized by RBM10) (PMID:40571778), and antagonizes RBM10 in NUMB splicing to suppress cancer cell proliferation and tumor growth (PMID:24332178). Beyond splicing, RBM5 stabilizes p53 protein post-transcriptionally and induces G1 arrest and mitochondrial apoptosis acting upstream of Bax (PMID:20309933, PMID:16585163). RBM5 expression is itself cross-regulated by RBM10-driven AS-NMD and its protein activity is controlled by phosphorylation and by PRPF19-mediated ubiquitination (PMID:28586478, PMID:16927403, PMID:40571778). In vivo, a missense mutation in RRM2 disrupts RNA binding and causes spermatid differentiation arrest and male sterility in mouse (PMID:23935508).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2000 Medium

    Establishing RBM5 as a nuclear RNA-binding protein with growth-suppressive activity set the foundation for its role as a tumor suppressor acting on RNA.

    Evidence Epitope-tag localization, in vitro poly(G) RNA binding, and ectopic expression growth assays in fibrosarcoma cells

    PMID:11029660

    Open questions at the time
    • RNA targets undefined
    • molecular mechanism of growth suppression not established
    • splicing role not yet identified
  2. 2006 Medium

    Defining the apoptotic output of RBM5 placed it functionally upstream of the mitochondrial cell-death pathway, explaining its tumor-suppressor phenotype.

    Evidence Stable overexpression in A549 cells with caspase/cytochrome c readouts and Bax siRNA epistasis

    PMID:16585163

    Open questions at the time
    • direct molecular targets linking RBM5 to Bax not identified
    • single cell line
    • does not distinguish splicing from non-splicing mechanism
  3. 2008 High

    Two parallel studies converted RBM5 from a generic RNA-binder into a sequence-specific splicing regulator, showing it controls apoptotic isoform choice of FAS and caspase-2.

    Evidence In vitro spliceosome assembly assays with OCRE mutagenesis (FAS) and yeast RNA interaction plus in vitro/cellular splicing assays with binding-site mutagenesis (caspase-2)

    PMID:18840686 PMID:18851835

    Open questions at the time
    • protein partners mediating the spliceosomal arrest not fully resolved
    • genome-wide target spectrum unknown
  4. 2010 Medium

    Connecting RBM5 to post-transcriptional p53 stabilization expanded its tumor-suppressor activity beyond splicing into protein-level control of a master regulator.

    Evidence Reporter assays, shRNA knockdown and overexpression with p53 target gene readouts after DNA damage

    PMID:20309933

    Open questions at the time
    • direct mechanism of p53 protein stabilization unknown
    • no direct binding partner identified
  5. 2012 High

    Structural and biochemical dissection assigned discrete functions to RBM5 domains: RRM2 RNA recognition, Zf1 RNA binding/druggability, and G-patch-dependent stimulation of the DHX15 helicase within the spliceosome.

    Evidence NMR solution structures of RRM2 and Zf1, fragment screening, co-IP/pull-down with DHX15 and PRP19, and in vitro helicase assays with domain mutagenesis

    PMID:22162216 PMID:22569250 PMID:22839758

    Open questions at the time
    • how DHX15 stimulation maps onto specific splicing events not resolved
    • Zf1 ligand has no cellular validation
  6. 2012 High

    Identifying U2AF65 competition at weak 3' splice sites provided a concrete biochemical mechanism for RBM5-mediated exon skipping.

    Evidence Minigene splicing in HeLa and in vitro U2AF65 competition assays on AID pre-mRNA

    PMID:23017209

    Open questions at the time
    • generality across endogenous targets not shown in this study
    • structural basis of competition not defined
  7. 2013 High

    RBM5 was shown to govern proliferation through splicing of the Notch regulator NUMB, antagonizing RBM10, and an in vivo mutation established its physiological splicing role in spermatogenesis.

    Evidence Minigene/RNA-seq/knockdown plus colony-formation and xenograft assays (NUMB); mouse genetic R263P RRM2 mutant with RNA pull-down and splicing phenotyping (testis)

    PMID:23935508 PMID:24332178

    Open questions at the time
    • positional rules distinguishing RBM5 from RBM10 not fully mechanistic
    • in vivo target set incompletely defined
  8. 2016 High

    Solving the OCRE–SmN complex structure revealed how RBM5 physically contacts the spliceosome to dictate FAS splice-site pairing.

    Evidence NMR structure of OCRE–SmN peptide complex with aromatic-residue mutagenesis and functional splicing assays

    PMID:27894420

    Open questions at the time
    • how Sm contact translates into the kinetic spliceosomal block not fully resolved
  9. 2017 Medium

    Reciprocal cross-regulation between RBM5 and RBM10 was established, with RBM10 triggering AS-NMD of RBM5 and RBM5 binding specific RBM10 variants, defining a feedback circuit between the paralogs.

    Evidence Computational prediction with RT-PCR/NMD reporters (RBM10→RBM5) and RIP-seq plus Western blot (RBM5→RBM10)

    PMID:28586478 PMID:28662214

    Open questions at the time
    • physiological contexts of the circuit not defined
    • single-lab studies
  10. 2018 Medium

    Genetic epistasis in C. elegans confirmed a conserved functional relationship between RBM5 and U2AF, reinforcing the U2AF-competition model at weak 3' splice sites.

    Evidence rbm-5/uaf-1 genetic suppression screen with transcriptome analysis and neuronal tissue-specific rescue

    PMID:30295127

    Open questions at the time
    • gene-specific suppress-vs-enhance behavior not mechanistically explained
    • ortholog system
  11. 2024 High

    Native spliceosome isolation placed RBM5/RBM10 directly on the U2 snRNP at branch sites transcriptome-wide, unifying prior single-target studies into a genome-scale repression mechanism.

    Evidence Chromatin-associated spliceosome isolation, branch-site RNA-seq, and conserved-peptide deletion mutagenesis

    PMID:37790489 PMID:38537639

    Open questions at the time
    • how branch-site engagement selects which exons are repressed not fully resolved
  12. 2024 Medium

    Multiple studies extended RBM5 into non-splicing and disease-context roles—mRNA stabilization (PTEN), m6A regulation via FTO, ER-stress pathway output, and a noncanonical DNA-binding-dependent maintenance of HOXA9 transcription in AML.

    Evidence RIP/half-life/luciferase and xenograft (PTEN); RIP-seq/co-IP and methylation assays (FTO); ubiquitination/epistasis (eIF2α); CRISPR/degron/domain screens (HOXA9)

    PMID:38216972 PMID:38272235 PMID:38742685 PMID:39072172

    Open questions at the time
    • direct DNA binding by RBM5 not biochemically demonstrated
    • FTO and eIF2α links are low-confidence single-lab findings
    • relationship between splicing and these noncanonical roles unclear
  13. 2025 Medium

    A detailed domain-level model showed how a lncRNA cofactor and PRPF19 jointly tune RBM5's FAS-splicing activity and its own stability.

    Evidence Domain mutagenesis mapping ZnF-C2H2 and other residues, RNA pull-down, co-IP, minigene splicing, ubiquitination assays, and xenograft (MGC32805/FAS/PRPF19)

    PMID:40571778

    Open questions at the time
    • generality of lncRNA-assisted recruitment to other targets unknown
    • single-lab study

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RBM5 partitions between its canonical U2 snRNP/branch-site splicing function and its reported noncanonical roles (DNA-binding transcriptional control, mRNA stability, m6A regulation) remains unresolved.
  • no biochemical demonstration of direct DNA binding
  • no unified model integrating splicing and non-splicing activities
  • structural basis of full-length RNA target selection in cells undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 2
Pathway
R-HSA-5357801 Programmed Cell Death 2 R-HSA-8953854 Metabolism of RNA 2
Partners
DHX15FTOPRPF19RBM10SNRPBU2AF2
Complex memberships
U2 snRNP

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 RBM5 regulates alternative splicing of Fas pre-mRNA by inhibiting the transition between prespliceosomal complexes assembled around exon 6 to mature spliceosomes assembled on flanking introns, promoting sequence-specific pairing of distal splice sites after exon definition rather than affecting early splice site recognition events. Biochemical spliceosome assembly assays, RNA-protein complex analysis, mutagenesis of OCRE domain Molecular cell High 18851835
2008 RBM5 binds to a U/C-rich sequence immediately upstream of the In100 splicing repressor element in caspase-2 pre-mRNA, and this binding enhances production of the proapoptotic Casp-2L isoform; mutagenesis of the binding site disrupts the ratio of proapoptotic/antiapoptotic isoforms. Yeast RNA-protein interaction assay, in vitro splicing assay, transfection in cells, mutagenesis Proceedings of the National Academy of Sciences of the United States of America High 18840686
2016 The RBM5 OCRE domain adopts a unique β-sheet fold and directly binds the proline-rich C-terminal tail of spliceosomal SmN/B/B' proteins; NMR structure of the OCRE–SmN peptide complex reveals specific recognition of poly-proline helical motifs; mutation of conserved aromatic residues in OCRE impairs Sm protein binding in vitro and compromises RBM5-mediated FAS/CD95 alternative splicing regulation. NMR structure determination, biochemical binding assays, mutagenesis, functional splicing assays eLife High 27894420
2023 Crystal/NMR structure of RBM5 RRM1-ZnF1-RRM2 domains in complex with RNA shows that RRM1-ZnF1 cooperate as a single structural module to sandwich target RNA and specifically recognize a GG dinucleotide in a non-canonical fashion; RRM2 is connected by a flexible linker but participates in RNA binding, adopting a closed architecture upon RNA engagement. X-ray crystallography, NMR, biochemical RNA binding assays Nature communications High 37454201
2012 RBM5 solution structure of the second RRM domain (RRM2) reveals it can preferentially bind both CU-rich and GA-rich RNA sequences with affinity in the 10^-5 molar range; both N- and C-terminal regions flanking the β-sheet surface participate in recognition. NMR solution structure determination, RNA binding assays Biochemistry High 22839758
2012 RBM5 directly interacts with the DExD/H-box helicase DHX15 and with PRP19 (two spliceosome components); the G-patch domain of RBM5 is required for DHX15 interaction; RBM5 stimulates DHX15 helicase activity in vitro in a G-patch domain-dependent manner. Co-immunoprecipitation, pull-down assays, in vitro helicase activity assay, domain mutagenesis FEBS letters High 22569250
2024 RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites across the transcriptome on chromatin; deletion of a conserved RBM5/RBM10 peptide containing a zinc finger motif disrupts U2 snRNP interaction and renders RBM5 inactive for repression of many alternative exons. Chromatin-associated spliceosome isolation, RNA sequencing of branch-site protected fragments, domain deletion mutagenesis, transcriptome-wide analysis Molecular cell High 37790489 38537639
2013 RBM5, RBM6, and RBM10 antagonistically regulate alternative splicing of the Notch pathway regulator NUMB pre-mRNA; RBM5/RBM6 and RBM10 show distinct positional effects on splicing; modulation of NUMB splicing recapitulates the effects of these factors on cancer cell colony formation and xenograft tumor growth. Minigene splicing assays, RNA-seq, siRNA knockdown, colony formation, xenograft assays Molecular cell High 24332178
2013 In mouse testes, RBM5 localizes to spermatogonia, spermatocytes, and round spermatids; a missense mutation R263P in the second RRM of RBM5 compromises mRNA binding and causes defective pre-mRNA splicing of target transcripts in round spermatids, leading to spermatid differentiation arrest and male sterility. Genetic screen, RNA pull-down with microarrays, immunofluorescence/localization, RT-PCR splicing assays, molecular modelling PLoS genetics High 23935508
2012 RBM5 promotes AID exon 4 skipping by competing with U2AF65 binding to the polypyrimidine tract at the 3' splice site of intron 3; this competition was demonstrated in vitro; a weak 3' splice site is required for the inhibitory effect. Minigene splicing assay in HeLa cells, in vitro competition assay for U2AF65 binding FEBS letters High 23017209
2017 RBM10 promotes alternative splicing-coupled nonsense-mediated decay (AS-NMD) of RBM5 mRNA by promoting skipping of RBM5 exon 6 or exon 16; this cross-regulation was validated computationally and experimentally, establishing a mechanism for RBM10-mediated cross-regulation of RBM5 expression. Computational prediction, RT-PCR splicing validation, NMD reporter assays, overexpression and knockdown experiments Nucleic acids research Medium 28586478
2017 RBM5 post-transcriptionally regulates RBM10 expression via direct interaction with specific RBM10 splice variants, as demonstrated by RNA immunoprecipitation followed by next-generation sequencing (RIP-Seq) and Western blotting. RIP-Seq, Western blotting, stable cell line with endogenous RBM5 deletion PloS one Medium 28662214
2018 In C. elegans, rbm-5 mutations suppress the temperature-sensitive lethality of a U2AF large subunit (uaf-1) mutant; rbm-5 mutations can suppress or enhance altered splicing of uaf-1-dependent genes in a gene-specific manner, with a preference at weak 3' splice sites; neuronal expression of rbm-5 was sufficient to rescue the suppression. Genetic epistasis screen, transcriptome analysis, tissue-specific rescue experiments RNA biology Medium 30295127
2010 RBM5 augments p53 transcriptional activity: RBM5 overexpression increases mRNA and protein levels of endogenous p53 target genes, and shRNA knockdown decreases them; RBM5 affects p53 protein but not mRNA levels after DNA damage, suggesting a post-transcriptional mechanism of p53 stabilization. Reporter gene assays, shRNA knockdown, Western blotting, RT-PCR, overexpression studies International journal of cancer Medium 20309933
2006 Stable overexpression of RBM5/H37 in A549 lung cancer cells induces G1 cell cycle arrest (decreased cyclin A and phospho-RB) and apoptosis via the mitochondrial pathway (increased Bax, cytochrome c release, caspase-9 and caspase-3 activation); Bax siRNA knockdown reduces RBM5-mediated apoptosis, placing Bax functionally downstream of RBM5. Stable transfection, Western blotting, mitochondrial membrane potential assay, cytochrome c fractionation, caspase activity assays, siRNA epistasis Cancer research Medium 16585163
2007 RBM5 is a phosphoprotein; phosphatase treatment decreases its apparent molecular mass; phosphorylation changes reversibly with growth factor addition/deprivation; mutagenesis and immunoprecipitation indicate serine 69 is phosphorylated under normal growth conditions (not by Akt kinase), with no evidence of phosphotyrosines. Phosphatase treatment (CIP), immunoprecipitation, site-directed mutagenesis, Western blotting Cell biochemistry and function Medium 16927403
2014 The two RRM domains (aa 98-178 and 231-315) of RBM5 are required for RNA binding; a mutant lacking both RRMs (RBM5-ΔRRM) fails to bind RNA, shows compromised caspase-2 alternative splicing activity, and lacks cell proliferation inhibition and apoptosis induction in A549 cells. Domain deletion mutagenesis, RNA binding assays, splicing assays, cell proliferation and apoptosis assays Biochemical and biophysical research communications Medium 24486491
2000 LUCA15/RBM5 protein localizes to the nucleus and binds poly(G) RNA homopolymers in vitro; ectopic expression of LUCA15 in HT1080 fibrosarcoma cells suppresses cell growth; LUCA15 mRNA is downregulated by constitutively active Ras(G12V). Epitope-tag localization, in vitro RNA binding assay, ectopic expression cell growth assay Genes to cells : devoted to molecular & cellular mechanisms Medium 11029660
2011 The RanBP2-type zinc finger 1 (Zf1) of RBM5 adopts a defined solution structure; NMR screening identified a small molecule (compound 1) that binds within the RNA binding pocket of the Zf1 domain, competing with RNA binding. NMR solution structure, NMR fragment screening, competitive binding assay Chembiochem : a European journal of chemical biology Medium 22162216
2020 The RanBP2-type zinc finger 1 (Zf1) of RBM5 harbors a non-canonical cysteine that kinetically destabilizes the zinc coordination; mutation of this cysteine establishes high-affinity zinc coordination, indicating that the native Zf1 has intrinsically dynamic metal exchange kinetics. NMR structure/dynamics analysis, metal-exchange kinetics, site-directed mutagenesis Journal of molecular biology Medium 32450081
2022 The L. monocytogenes virulence factor InlP translocates into human cell nuclei, interacts directly with RBM5 (identified by yeast two-hybrid, immunoprecipitation, and pull-down), inhibits RBM5-induced cell death, and stimulates formation of RBM5-containing nuclear granules where the SC35 speckle protein redistributes. Yeast two-hybrid, co-immunoprecipitation, pull-down, ectopic expression, cell death assay, immunofluorescence Scientific reports Medium 36535993
2024 In AML cells, RBM5 functions through a noncanonical transcriptional regulation mechanism (dependent on DNA-binding domains) rather than RNA splicing to maintain HOXA9 transcription; acute degradation of RBM5 via an auxin-inducible degron immediately reduces HOXA9 transcription; ectopic HOXA9 rescues impaired proliferation upon RBM5 loss. Genome-wide CRISPR/Cas9 screen, domain CRISPR screening, shRNA knockdown, auxin-inducible protein degradation, epistasis rescue experiments Genome biology Medium 38216972
2024 RBM5 binds directly to PTEN mRNA and stabilizes its expression; RBM5 overexpression increases PTEN protein, decreases PI3K and p-AKT, and suppresses glycolysis, proliferation, and metastasis of CRC cells; PTEN knockdown partially reverses RBM5-mediated suppression. RNA immunoprecipitation (RIP), half-life RT-PCR, dual-luciferase reporter assay, Western blotting, xenograft model, siRNA epistasis World journal of gastrointestinal oncology Medium 39072172
2024 RBM5 interacts with the m6A demethylase FTO in normal (cisplatin-sensitive) NSCLC cells but not in cisplatin-resistant cells; loss of RBM5 causes FTO to aberrantly methylate 5'-UTRs of resistance-associated transcripts, silencing their translation. RIP-seq, co-immunoprecipitation, translational activity assays, in vivo/in vitro m6A methylation analysis Journal of cellular physiology Low 38742685
2024 CHIP (E3 ubiquitin ligase) mediates ubiquitination and degradation of non-phosphorylated eIF2α; this promotes transcriptional upregulation of RBM5 (via ATF), and elevated RBM5 suppresses A549 cell proliferation and migration; this places RBM5 downstream of the PERK-eIF2α pathway under ER stress. In vitro ubiquitination assay, co-immunoprecipitation, reporter assay, siRNA knockdown, Western blotting The Journal of biological chemistry Low 38272235
2025 RBM5 recruits lncRNA MGC32805 and together they promote exclusion of FAS exon 6, decreasing apoptotic mFAS and increasing anti-apoptotic ΔFAS isoforms; RBM5 ZnF-C2H2 residues Leu650 and Arg681 recognize a 'GUACG' motif in MGC32805; His665 and Leu668 of RBM5 bind the FAS exon 6 adjacent element 'GAACAAA'; MGC32805 blocks the PRPF19 binding site (Lys645) on RBM5, preventing its ubiquitination and degradation. Mutagenesis, RNA pull-down, co-immunoprecipitation, minigene splicing assay, xenograft model, ubiquitination assay Oncogene Medium 40571778
2023 RBM5 binds directly to CARM1 mRNA and participates in alternative splicing-coupled nonsense-mediated decay (AS-NMD) to downregulate CARM1 expression in bladder cancer cells; reduced RBM5 leads to CARM1 upregulation, which activates the PRKACA/GSK3β/Wnt-β-catenin axis. RNA immunoprecipitation, AS-NMD reporter assays, Western blotting, pathway epistasis Cancers Low 38201567

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2013 RBM5, 6, and 10 differentially regulate NUMB alternative splicing to control cancer cell proliferation. Molecular cell 294 24332178
2008 RBM5/Luca-15/H37 regulates Fas alternative splice site pairing after exon definition. Molecular cell 145 18851835
2008 Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5. Proceedings of the National Academy of Sciences of the United States of America 108 18840686
2006 3p21.3 tumor suppressor gene H37/Luca15/RBM5 inhibits growth of human lung cancer cells through cell cycle arrest and apoptosis. Cancer research 72 16585163
2002 A candidate tumor suppressor gene, H37, from the human lung cancer tumor suppressor locus 3p21.3. Cancer research 71 12036935
2010 RBM5 as a putative tumor suppressor gene for lung cancer. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 68 20186023
2016 RBM5-AS1 Is Critical for Self-Renewal of Colon Cancer Stem-like Cells. Cancer research 66 27520449
2013 RBM5 is a male germ cell splicing factor and is required for spermatid differentiation and male fertility. PLoS genetics 65 23935508
2012 Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity. FEBS letters 54 22569250
2007 Expression of RBM5-related factors in primary breast tissue. Journal of cellular biochemistry 54 17131366
2000 LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells. Genes to cells : devoted to molecular & cellular mechanisms 54 11029660
2017 Autoregulation of RBM10 and cross-regulation of RBM10/RBM5 via alternative splicing-coupled nonsense-mediated decay. Nucleic acids research 49 28586478
2000 LUCA-15-encoded sequence variants regulate CD95-mediated apoptosis. Oncogene 49 10949932
1986 Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv. Archives of microbiology 46 3087325
2015 RBM5 and p53 expression after rat spinal cord injury: implications for neuronal apoptosis. The international journal of biochemistry & cell biology 45 25578565
2004 LUCA-15/RBM5, a putative tumour suppressor, enhances multiple receptor-initiated death signals. Apoptosis : an international journal on programmed cell death 45 15192330
2003 Simultaneous acceleration of the cell cycle and suppression of apoptosis by splice variant delta-6 of the candidate tumour suppressor LUCA-15/RBM5. Genes to cells : devoted to molecular & cellular mechanisms 42 12581154
2022 Hypoxia-induced lncRNA RBM5-AS1 promotes tumorigenesis via activating Wnt/β-catenin signaling in breast cancer. Cell death & disease 37 35110544
2014 An overview to understand the role of PE_PGRS family proteins in Mycobacterium tuberculosis H37 Rv and their potential as new drug targets. Biotechnology and applied biochemistry 36 24975480
2002 Candidate tumour suppressor LUCA-15 can regulate multiple apoptotic pathways. Apoptosis : an international journal on programmed cell death 36 12207175
2017 miR-483-5p promotes prostate cancer cell proliferation and invasion by targeting RBM5. International braz j urol : official journal of the Brazilian Society of Urology 35 28727371
2017 RBM10 promotes transformation-associated processes in small cell lung cancer and is directly regulated by RBM5. PloS one 33 28662214
2009 Characterization of the Rv3377c gene product, a type-B diterpene cyclase, from the Mycobacterium tuberculosis H37 genome. Chembiochem : a European journal of chemical biology 33 19618417
2001 LUCA-15 suppresses CD95-mediated apoptosis in Jurkat T cells. Oncogene 33 11420683
2012 3p21.3 tumor suppressor gene RBM5 inhibits growth of human prostate cancer PC-3 cells through apoptosis. World journal of surgical oncology 32 23158838
2007 RBM6-RBM5 transcription-induced chimeras are differentially expressed in tumours. BMC genomics 32 17908320
2006 Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes. Experimental cell research 32 16546166
2010 RBM5/H37 tumor suppressor, located at the lung cancer hot spot 3p21.3, alters expression of genes involved in metastasis. Lung cancer (Amsterdam, Netherlands) 30 20338664
2019 Down-regulated RBM5 inhibits bladder cancer cell apoptosis by initiating an miR-432-5p/β-catenin feedback loop. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29 31318608
2019 Long non-coding RNA RBM5-AS1 promotes the aggressive behaviors of oral squamous cell carcinoma by regulation of miR-1285-3p/YAP1 axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 29 31869662
2016 Structural basis for the recognition of spliceosomal SmN/B/B' proteins by the RBM5 OCRE domain in splicing regulation. eLife 29 27894420
2017 In vivo evidence that RBM5 is a tumour suppressor in the lung. Scientific reports 28 29176597
2012 Differential expression of RBM5, EGFR and KRAS mRNA and protein in non-small cell lung cancer tissues. Journal of experimental & clinical cancer research : CR 28 22537942
2009 Identification and characterisation of a novel antisense non-coding RNA from the RBM5 gene locus. Gene 28 19559772
2004 Genetic instability of RBM5/LUCA-15/H37 in MCF-7 breast carcinoma sublines may affect susceptibility to apoptosis. Cell biochemistry and function 28 15338470
2024 The splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. Molecular cell 26 38537639
2017 RBM5 inhibits tumorigenesis of gliomas through inhibition of Wnt/β-catenin signaling and induction of apoptosis. World journal of surgical oncology 25 28061901
2012 The tumor suppressor gene RBM5 inhibits lung adenocarcinoma cell growth and induces apoptosis. World journal of surgical oncology 23 22866867
2010 p53 transactivation is involved in the antiproliferative activity of the putative tumor suppressor RBM5. International journal of cancer 23 20309933
2012 Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets. Biochemistry 21 22839758
2023 Structural basis for specific RNA recognition by the alternative splicing factor RBM5. Nature communications 20 37454201
2015 Cloning and characterization of a novel PE_PGRS60 protein (Rv3652) of Mycobacterium tuberculosis H37 Rv exhibit fibronectin-binding property. Biotechnology and applied biochemistry 20 26158346
2013 Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways. World journal of surgical oncology 20 23721095
2012 RBM5 promotes exon 4 skipping of AID pre-mRNA by competing with the binding of U2AF65 to the polypyrimidine tract. FEBS letters 20 23017209
2016 Overexpression of RBM5 induces autophagy in human lung adenocarcinoma cells. World journal of surgical oncology 19 26923134
2005 Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37 Rv. Journal of applied microbiology 19 15752328
2012 The 3p21.3 tumor suppressor RBM5 resensitizes cisplatin-resistant human non-small cell lung cancer cells to cisplatin. Cancer epidemiology 18 22609235
2006 The antiapoptotic RBM5/LUCA-15/H37 gene and its role in apoptosis and human cancer: research update. TheScientificWorldJournal 17 17195868
2020 RNA Binding Motif 5 (RBM5) in the CNS-Moving Beyond Cancer to Harness RNA Splicing to Mitigate the Consequences of Brain Injury. Frontiers in molecular neuroscience 16 32765218
2012 Differential expression of RBM5 and KRAS in pancreatic ductal adenocarcinoma and their association with clinicopathological features. Oncology letters 16 23425895
2021 RBM5-AS1 promotes radioresistance in medulloblastoma through stabilization of SIRT6 protein. Acta neuropathologica communications 15 34225779
2019 Long noncoding RNA AFAP1-AS1 accelerates the proliferation and metastasis of prostate cancer via inhibiting RBM5 expression. European review for medical and pharmacological sciences 15 31081081
2014 The RNA recognition motif domains of RBM5 are required for RNA binding and cancer cell proliferation inhibition. Biochemical and biophysical research communications 15 24486491
2013 Differential downregulation of Rbm5 and Rbm10 during skeletal and cardiac differentiation. In vitro cellular & developmental biology. Animal 15 24178303
1991 The serodiagnosis of tuberculosis in children: an evaluation of an ELISA test using IgG antibodies to M. tuberculosis, strain H37 RV. Annals of tropical paediatrics 15 1715140
2016 RBM5 reduces small cell lung cancer growth, increases cisplatin sensitivity and regulates key transformation-associated pathways. Heliyon 13 27957556
2011 Targeting zinc finger domains with small molecules: solution structure and binding studies of the RanBP2-type zinc finger of RBM5. Chembiochem : a European journal of chemical biology 12 22162216
2008 Death by splicing: tumor suppressor RBM5 freezes splice-site pairing. Molecular cell 12 18951082
2002 RBM5/LUCA-15--tumour suppression by control of apoptosis and the cell cycle? TheScientificWorldJournal 12 12920317
2019 Long noncoding RNA SNHG7 represses the expression of RBM5 to strengthen metastasis of hepatocellular carcinoma. European review for medical and pharmacological sciences 11 31298322
2018 RBM5-AS1 participates in fracture healing and inhibits apoptosis of bone cells through the up-regulation of β-catenin. European review for medical and pharmacological sciences 11 30178827
2023 Downregulated RBM5 Enhances CARM1 Expression and Activates the PRKACA/GSK3β Signaling Pathway through Alternative Splicing-Coupled Nonsense-Mediated Decay. Cancers 10 38201567
2022 hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis. Disease markers 10 36193508
2021 lncRNA RBM5-AS1 promotes cell proliferation and invasion by epigenetically silencing miR-132/212 in hepatocellular carcinoma cells. Cell biology international 10 34019714
2020 RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling. European neurology 10 32610314
2014 Lentiviral vector-mediated RBM5 overexpression downregulates EGFR expression in human non-small cell lung cancer cells. World journal of surgical oncology 10 25441176
2020 Identification of Novel Targets of RBM5 in the Healthy and Injured Brain. Neuroscience 9 32335213
2007 The apoptosis modulator and tumour suppressor protein RBM5 is a phosphoprotein. Cell biochemistry and function 9 16927403
2024 RNA-binding protein RBM5 plays an essential role in acute myeloid leukemia by activating the oncogenic protein HOXA9. Genome biology 8 38216972
2021 LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion. BioMed research international 8 33816613
2007 The two single nucleotide polymorphisms in the H37/RBM5 tumour suppressor gene at 3p21.3 correlated with different subtypes of non-small cell lung cancers. Lung cancer (Amsterdam, Netherlands) 8 17606309
2024 FTO blocks RNA translational activity via the loss of N6-methyladenosine methylation at 5' UTR regulated by RBM5 in cisplatin-resistant NSCLC. Journal of cellular physiology 7 38742685
2019 LncRNA AB073614 promotes the proliferation and inhibits apoptosis of cervical cancer cells by repressing RBM5. European review for medical and pharmacological sciences 7 30964162
2018 RBM-5 modulates U2AF large subunit-dependent alternative splicing in C. elegans. RNA biology 7 30295127
2009 Comparison of the activities of BM2 protein and its H19 and W23 mutants of influenza B virus with activities of M2 protein and its H37 and W41 mutants of influenza A virus. Archives of virology 7 19763781
2022 A bacterial virulence factor interacts with the splicing factor RBM5 and stimulates formation of nuclear RBM5 granules. Scientific reports 6 36535993
2019 miR-938 promotes cell proliferation by regulating RBM5 in lung adenocarcinoma cells. Cell biology international 6 31498514
2019 Long noncoding RNA PCAT-1 accelerates the metastasis of pancreatic cancer by repressing RBM5. European review for medical and pharmacological sciences 6 31539121
2018 Effect of miR-483-5p on apoptosis of lung cancer cells through targeting of RBM5. International journal of clinical and experimental pathology 6 31938444
2021 Domain-wise differentiation of Mycobacterium tuberculosis H37 Rv hypothetical proteins: A roadmap to discover bacterial survival potentials. Biotechnology and applied biochemistry 5 33469971
2020 Conformational Dynamics from Ambiguous Zinc Coordination in the RanBP2-Type Zinc Finger of RBM5. Journal of molecular biology 5 32450081
2015 Post-transcriptional regulation of Rbm5 expression in undifferentiated H9c2 myoblasts. In vitro cellular & developmental biology. Animal 5 26659391
2025 Plasma expression of antisense LncRNAs RBM5-AS1, VPS9D1-AS1 and STEAP3-AS1 as novel biomarkers for colorectal cancer diagnosis. Molecular biology reports 4 40522493
2024 CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5. The Journal of biological chemistry 4 38272235
2024 RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells via stabilizing phosphatase and tensin homolog mRNA. World journal of gastrointestinal oncology 4 39072172
2020 Comprehensive analysis of GTP cyclohydrolase I activity in Mycobacterium tuberculosis H37 Rv via in silico studies. Biotechnology and applied biochemistry 4 32691412
2017 Association of RBM5 rs2013208 SNP with serum lipid levels in two Chinese ethnic groups. International journal of clinical and experimental pathology 4 31966706
2012 Downregulating activated epidermal growth factor receptor has no effect on RBM5 expression. Chinese medical journal 4 22882865
2007 Promoter methylation study of the H37/RBM5 tumor suppressor gene from the 3p21.3 human lung cancer tumor suppressor locus. Human genetics 4 18038152
2023 The apoptotic splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. bioRxiv : the preprint server for biology 3 37790489
2023 Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner. Cell death discovery 3 37848418
2020 LncRNA LINP1 promotes proliferation and inhibits apoptosis of gastric cancer cells by repressing RBM5. European review for medical and pharmacological sciences 3 31957826
2020 OCRE Domains of Splicing Factors RBM5 and RBM10: Tyrosine Ring-Flip Frequencies Determined by Integrated Use of 1 H NMR Spectroscopy and Molecular Dynamics Simulations. Chembiochem : a European journal of chemical biology 3 32975902
2025 RBM5 recruiting MGC32805 in a sandwich mode and inducing ΔFAS neoantigen and triggering FAS properties switch: implication in colorectal cancer. Oncogene 2 40571778
2024 PEI, a new transfection method, augments the inhibitory effect of RBM5 on prostate cancer. Biochemical and biophysical research communications 2 38402723
2024 ZNF554 Inhibits Endometrial Cancer Progression via Regulating RBM5 and Inactivating WNT/β-Catenin Signaling Pathway. Current medical science 2 38619681
2023 Elucidating the role of RBM5 in osteoclastogenesis: a novel potential therapeutic target for osteoporosis. BMC musculoskeletal disorders 2 38031049
2014 [Construction of RBM5 vector, establishment of stably transfected A549 cell line and preliminary research on the function of RBM5 gene]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 2 25355266
2024 Circular RNA LDLRAD3 promotes gastric cancer progression by upregulating COL4A5 through sponging miR-h37. Journal of the Chinese Medical Association : JCMA 1 39161132
2023 RNA-binding protein PUM2 promotes T-cell acute lymphoblastic leukemia via competitively binding to RBM5 3'UTR with miR-28-5p. European journal of haematology 1 36536516

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