Affinage

RBM47

RNA-binding protein 47 · UniProt A0AV96

Length
593 aa
Mass
64.1 kDa
Annotated
2026-06-10
47 papers in source corpus 37 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBM47 is a multi-domain RNA-binding protein that acts as a broadly-acting post-transcriptional regulator, governing mRNA editing, stability, and splicing across diverse tissues and disease contexts (PMID:24898756, PMID:24916387). It is the essential cofactor for APOBEC1-mediated C-to-U RNA editing, physically interacting with APOBEC1 and substituting for A1CF; intestine- and liver-specific Rbm47 knockouts abolish or reduce apoB and other target editing, while RBM47/A1CF act both independently and in tissue-specific combination to dictate editing site selection (PMID:24916387, PMID:30309881, PMID:30844405). Through its RRM domains, RBM47 recognizes (U)GCAUG and AU-rich elements to control alternative splicing — promoting TJP1 exon-20 inclusion via its first RRM to restrain cell migration, and directing tissue-specific SEC31A splicing that governs large lipid-cargo secretion (PMID:31358901, PMID:40436629). The dominant theme across its targets is sequence-specific binding to 3'UTRs that stabilizes (or, for select transcripts, destabilizes) mRNAs: it stabilizes IFNAR1 to amplify interferon-stimulated antiviral responses (PMID:34160127), stabilizes Wnt-pathway and growth-suppressive transcripts including DKK1 and AXIN1 (PMID:24898756, PMID:32891348), and destabilizes pro-tumorigenic transcripts such as ITGAV and PD-L1 (PMID:40638600, PMID:41376900). RBM47 additionally functions at the promoter level, controlling transcription of p53 — thereby setting the p53–p21 cell-fate switch after DNA damage — and of UPF1 (PMID:31511650, PMID:35831298). RBM47 itself is regulated by ISGylation at K329 (primed by S309 phosphorylation), which tunes immune activation and tumor susceptibility, by OTUD4-mediated deubiquitination that stabilizes the protein, and transcriptionally by FOXA1, RUNX1, and HDAC2/H3K27 deacetylation (PMID:38036512, PMID:38553613, PMID:41407921, PMID:41335176). Genetic loss-of-function in mice establishes RBM47 as required for embryonic viability and intestinal homeostasis, where its absence drives aberrant proliferation, altered Wnt signaling, and spontaneous polyposis (PMID:26789794, PMID:37014710). Through these combined activities RBM47 behaves as a context-dependent tumor suppressor or oncogene.

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2013 Medium

    Established that RBM47 is a bona fide RNA-binding (not ssDNA-binding) protein with developmental function, placing it upstream of Wnt8a in head formation.

    Evidence In vitro RNA binding assay and zebrafish morpholino knockdown rescued by wnt8a morpholino

    PMID:24038582

    Open questions at the time
    • Direct mRNA targets mediating Wnt8a regulation not identified
    • Binding specificity/sequence preference undefined at this stage
  2. 2014 High

    Defined RBM47's transcriptome-wide binding landscape and a concrete tumor-suppressive mechanism, answering how it engages mRNAs to alter splicing and abundance.

    Evidence HITS-CLIP, mRNA stability assays, breast cancer models

    PMID:24898756

    Open questions at the time
    • Full set of functionally relevant targets beyond DKK1 not resolved
    • Determinants of stabilize-vs-destabilize outcome unclear
  3. 2014 High

    Identified RBM47 as a genuine APOBEC1 editing cofactor capable of replacing A1CF, redefining the in vivo machinery for C-to-U RNA editing.

    Evidence Co-IP, in vitro editing reconstitution, Rbm47 mutant mice

    PMID:24916387

    Open questions at the time
    • Structural basis of APOBEC1 engagement not determined
    • Relative in vivo contribution of RBM47 vs A1CF per tissue not yet quantified
  4. 2016 Medium

    Showed RBM47 is required cell-autonomously in the embryo proper for viability, establishing an essential developmental role.

    Evidence Conditional Cre/FLP gene-trap knockout mice with mid-gestation lethality

    PMID:26789794

    Open questions at the time
    • Molecular targets responsible for lethality not identified
    • Affected tissue/lineage not pinpointed
  5. 2018 High

    Resolved the tissue-specific division of labor between RBM47 and A1CF in dictating APOBEC1 editing site selection.

    Evidence Liver- and intestine-specific and double conditional knockout mice with editing quantification

    PMID:30309881

    Open questions at the time
    • Rules governing which sites require RBM47 vs A1CF undefined
    • Mechanism of cofactor cooperativity not structurally explained
  6. 2018 Medium

    Extended RBM47's role to immune regulation, showing AU-rich-element-mediated mRNA stabilization controls cytokine output.

    Evidence RIP, mRNA stability assay, IL-10/Treg functional assays in B cells

    PMID:29844590

    Open questions at the time
    • Single lab; physiological relevance in immune disease not tested
    • Binding specificity within Il10 3'UTR not mapped
  7. 2019 High

    Mapped a domain-specific splicing function, demonstrating the first RRM mediates (U)GCAUG-directed exon inclusion with migration consequences.

    Evidence Splicing reporters, RRM mutagenesis, migration assays on TJP1

    PMID:31358901

    Open questions at the time
    • Genome-wide splicing program beyond TJP1 incomplete
    • Cooperating splicing factors not defined here
  8. 2019 Medium

    Revealed an unexpected transcriptional role: RBM47 controls p53 promoter activity and thereby the p53-p21 death/senescence switch.

    Evidence siRNA knockdown, overexpression, luciferase promoter assays

    PMID:31511650

    Open questions at the time
    • Direct promoter binding vs indirect effect not fully distinguished
    • DNA-binding mode of RBM47 unresolved
  9. 2019 High

    Defined the minimal RBM47 domain requirement for editing and differential activity versus A1CF across targets and species.

    Evidence Cell-based fluorescence editing reconstitution with domain truncation in HEK293T

    PMID:30844405

    Open questions at the time
    • Atomic-resolution structure of editing complex absent
    • Basis of human-mouse activity differences unexplained
  10. 2020 Medium

    Consolidated RBM47's Wnt-suppressive role through AXIN1 mRNA stabilization in lung cancer, and revealed a divergent zebrafish role degrading MAVS to limit IFN.

    Evidence RIP, mRNA stability, knockdown tumor models (AXIN1); Co-IP and lysosome inhibitor assays (zebrafish MAVS)

    PMID:32859727 PMID:32891348

    Open questions at the time
    • Apparent opposing effects on innate immunity across systems unreconciled
    • Mechanism targeting MAVS to lysosome not molecularly defined
  11. 2021 High

    Established RBM47 as an interferon-inducible amplifier of antiviral immunity by stabilizing IFNAR1 mRNA, and added BCAT1 promoter and hnRNPM-cooperative splicing roles.

    Evidence Reciprocal RIP, mRNA stability, multiple virus infection models (IFNAR1); ChIP and Co-IP (BCAT1/hnRNPM)

    PMID:34160127 PMID:34274258

    Open questions at the time
    • How viral induction of RBM47 is wired upstream not fully defined
    • Scope of hnRNPM-cooperative splicing targets incomplete
  12. 2021 Medium

    Implicated RBM47 in stem-cell differentiation via FGF-ERK signaling and added growth-suppressive HMGA2 mRNA destabilization.

    Evidence ESC knockdown with FGFR/MEK inhibitor rescue, differentiation assays; RIP/mRNA stability for HMGA2

    PMID:34105460 PMID:35986129

    Open questions at the time
    • Direct RBM47 targets within FGF-ERK axis unidentified
    • HMGA2 finding (Low) is single RIP without independent validation
  13. 2022 Medium

    Demonstrated dual RNA-binding and transcription-factor activity on a single target (UPF1) and broadened the 3'UTR-stabilization repertoire to tumor-suppressive transcripts.

    Evidence RIP, mRNA stability, ChIP/reporter, xenografts (UPF1); RIP and rescue (PTEN, SNHG5/FOXO3)

    PMID:35338124 PMID:35831298 PMID:36321407

    Open questions at the time
    • Distinct DNA- vs RNA-binding determinants not separated
    • Feedback loop kinetics (FOXO3-RBM47) not quantified
  14. 2023 High

    Defined post-translational control of RBM47 by phosphorylation-primed ISGylation at K329, linking this modification to immune activation and tumor susceptibility in vivo.

    Evidence K329R knockin mice, site-specific nanobody-directed ISGylation, RNA-seq, immune assays

    PMID:38036512

    Open questions at the time
    • Kinase responsible for S309 priming not identified
    • How ISGylation alters RBM47 RNA target selection unresolved
  15. 2023 High

    Provided definitive in vivo evidence for RBM47 as an intestinal tumor suppressor controlling proliferation, Wnt, and colitis-associated polyposis.

    Evidence Intestine-specific knockout mice, organoids, colitis and ApcMin/+ models, RNA-seq

    PMID:37014710

    Open questions at the time
    • Which individual targets drive polyposis not isolated
    • Mechanism of colitis protection vs tumor promotion duality unclear
  16. 2024 Medium

    Identified OTUD4 as a deubiquitinase stabilizing RBM47, coupling protein turnover to ferroptosis regulation via ATF3.

    Evidence Co-IP, deubiquitination assay, mRNA stability, rescue in ccRCC

    PMID:38553613

    Open questions at the time
    • E3 ligase opposing OTUD4 not identified
    • Single lineage context (ccRCC) only
  17. 2025 Medium

    Expanded RBM47 target logic to context-dependent destabilization (ITGAV, PD-L1) and stabilization (cGAS, GSDMA, PDIA6, NEDD4L) controlling metastasis, immunity, and cell death.

    Evidence RIP/luciferase/mRNA-stability and in vivo functional assays across multiple cancers; RRM2-specific binding for cGAS

    PMID:39741300 PMID:40638600 PMID:41376900 PMID:41407921 PMID:41681975 PMID:42177907

    Open questions at the time
    • Determinants selecting stabilization vs destabilization across targets undefined
    • Several of these single-lab findings lack reciprocal validation
  18. 2025 Medium

    Clarified upstream transcriptional and splicing control of RBM47 itself, identifying FOXA1, RUNX1, and HDAC2/H3K27 deacetylation as regulators and CpG methylation as a silencing mechanism.

    Evidence ChIP, reporter, bisulfite sequencing, rescue assays (FOXA1, RUNX1, HDAC2)

    PMID:41027284 PMID:41335176 PMID:41407921

    Open questions at the time
    • Hierarchy among these upstream regulators not established
    • Context dependence of each regulator across tissues unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RBM47 chooses between mutually exclusive molecular outputs — editing cofactor, mRNA stabilizer, mRNA destabilizer, splicing factor, or promoter-bound transcriptional regulator — on a given transcript remains unresolved.
  • No structural model integrating RNA, DNA, and APOBEC1 binding
  • Rules linking post-translational state (ISGylation/ubiquitination) to target selection unknown
  • Mechanistic basis of context-dependent tumor-suppressor vs oncogene behavior undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0003677 DNA binding 3 GO:0140098 catalytic activity, acting on RNA 3 GO:0140110 transcription regulator activity 3
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 3 R-HSA-168256 Immune System 3 R-HSA-8953854 Metabolism of RNA 3
Partners
A1CFAPOBEC1HNRNPMOTUD4
Complex memberships
APOBEC1 RNA editing complex (APOBEC1-RBM47)

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2014 RBM47 binds broadly to mRNAs (most prominently in introns and 3'UTRs) as revealed by transcriptome-wide HITS-CLIP, and alters splicing and abundance of a subset of target mRNAs; one mechanism of tumor suppression involves stabilization of DKK1 mRNA to inhibit Wnt signaling and breast cancer progression. HITS-CLIP (transcriptome-wide), mRNA stability assays, functional breast cancer models eLife High 24898756
2014 RBM47 interacts with APOBEC1 and A1CF, can substitute for A1CF, and is necessary and sufficient for APOBEC1-mediated C-to-U RNA editing in vitro; Rbm47-deficient mice show impaired C-to-U RNA editing. Co-immunoprecipitation, in vitro RNA editing reconstitution assay, Rbm47 mutant mouse model EMBO reports High 24916387
2018 In mouse intestine and liver, RBM47 and A1CF function independently yet interact in a tissue-specific manner to regulate APOBEC1-dependent C-to-U RNA editing site selection; intestine-specific Rbm47 knockout nearly eliminates apoB RNA editing (<6%), while liver-specific knockout reduces editing of a subset of targets; double knockout of both A1cf and Rbm47 eliminates apoB RNA editing in liver. Tissue-specific conditional knockout mice (liver-specific and intestine-specific), RNA editing quantification of multiple targets RNA (New York, N.Y.) High 30309881
2019 RBM47 promotes inclusion of exon 20 (α-domain) of TJP1 pre-mRNA by recognizing (U)GCAUG sequences in the downstream intronic region; the first RRM domain of RBM47 is critical for this alternative splicing regulation. Loss of RBM47-mediated inclusion yields a TJP1-α− isoform that enhances actin stress fiber assembly and promotes cell migration. Splicing reporter assays, RRM domain mutagenesis, RNA binding assays, wound healing/migration assays Oncogene High 31358901
2019 RBM47 regulates p53 at the transcriptional level: RBM47 knockdown reduces p53 mRNA and protein by impairing p53 promoter activity, and RBM47 overexpression enhances p53 promoter activity. By controlling p53, RBM47 concomitantly controls p21WAF1/CIP1 expression at the transcriptional level, acting as a molecular switch between cell death and senescence after DNA damage. siRNA knockdown, Flag-RBM47 overexpression, luciferase promoter activity assays, Western blot, RT-PCR Cell death and differentiation Medium 31511650
2019 APOBEC1-RBM47 and APOBEC1-A1CF complexes reconstituted in HEK293T cells show differential RNA editing activity across multiple target RNAs; the minimal domain requirement of RBM47 for editing activity was determined, and clear species differences (mouse vs. human) were observed. Cell-based fluorescence RNA editing assay (eGFP subcellular localization), domain truncation/mutagenesis, reconstitution in HEK293T cells Journal of molecular biology High 30844405
2018 RBM47 promotes IL-10 mRNA stability in B cells by binding to AU-rich elements in the 3'UTR of Il10 mRNA, thereby elevating IL-10 production and enhancing immunosuppressive B cell function including Foxp3+ Treg induction. mRNA microarray, RNA immunoprecipitation (RIP), mRNA stability assay, overexpression functional assays Cellular & molecular immunology Medium 29844590
2020 RBM47 binds the AXIN1 mRNA and stabilizes it, thereby enhancing suppression of Wnt/β-catenin signaling in NSCLC cells; lentiviral knockdown of RBM47 reduces AXIN1 levels and promotes proliferation, migration, and invasion. RNA immunoprecipitation, mRNA stability assay, lentiviral knockdown, in vitro and in vivo functional assays Surgical oncology Medium 32891348
2021 RBM47 binds the 3'UTR of IFNAR1 mRNA, increases its stability, and retards IFNAR1 degradation, thereby enhancing IFN-stimulated gene expression and broad-spectrum antiviral activity; RBM47 expression is induced by viral infection or interferon stimulation and has no noticeable impact on IFN production itself. RNA immunoprecipitation, mRNA stability assay, multiple virus infection models in vitro and in vivo, ISRE reporter assay EMBO reports High 34160127
2021 RBM47 binds to the promoter of BCAT1 and regulates its transcription in NPC cells; RBM47 also cooperates with hnRNPM (confirmed by co-immunoprecipitation) to regulate alternative splicing of multiple pre-mRNAs. ChIP (chromatin immunoprecipitation), transcriptome analysis, Co-IP with hnRNPM, functional knockdown assays Journal of genetics and genomics Medium 34274258
2020 Zebrafish RBM47 interacts with MAVS and promotes its lysosome-dependent degradation, changing MAVS cellular localization from cytoplasm to the lysosome region, thereby inhibiting downstream MITA and IRF3/7 activation and suppressing IFN production. Co-immunoprecipitation, subcellular localization assays, lysosome inhibitor experiments, functional IFN reporter assays Journal of immunology Medium 32859727
2013 Human RBM47 specifically binds RNA but not single-stranded DNA; zebrafish rbm47 knockdown causes headless/small head phenotypes rescued by wnt8a morpholino, placing rbm47 function upstream of Wnt8a signaling in head development. RNA binding assay (in vitro), morpholino knockdown, genetic rescue with wnt8a morpholino, microarray Developmental dynamics Medium 24038582
2013 RNA immunoprecipitation in mouse ES cells showed that Rbm47 binds to the Nanog transcript but does not bind Sox2 or Oct4 transcripts. HA-tagged RBM47 overexpression, RNA-binding protein immunoprecipitation (RIP), RT-PCR Molecular biology reports Low 23649762
2016 Rbm47 function is required in the embryo proper (not the visceral endoderm) for viability and growth; conditional tissue-specific inactivation using Cre/FLP recombinase on a FlEx gene-trap cassette showed Rbm47gt/gt mutants die at mid-gestation. Conditional tissue-specific knockout mice (Cre/FLP recombinase system), embryonic lethal phenotyping Genesis (New York, N.Y.) Medium 26789794
2022 RBM47 stabilizes SNHG5 lncRNA by directly binding to it; SNHG5 in turn inhibits FOXO3 ubiquitination and degradation by recruiting USP21, promoting FOXO3 nuclear translocation and activation of autophagy-related genes (ATG3, ATG5). FOXO3 also activates RBM47 expression, forming a positive feedback loop. RIP assay, RNA stability assay, co-immunoprecipitation, immunofluorescence (FOXO3 localization), in vitro and in vivo functional assays Cell death & disease Medium 35338124
2022 RBM47 stabilizes UPF1 mRNA by binding its 3'UTR (acting as an RNA-binding protein), and also promotes UPF1 transcription (acting as a transcription factor/DNA-binding protein), thereby suppressing HCC progression. RNA immunoprecipitation, mRNA stability assay, ChIP/transcriptional reporter, in vitro and xenograft in vivo assays Cell death discovery Medium 35831298
2023 RBM47 undergoes ISGylation at lysine 329 in a phosphorylation-dependent manner (S309 phosphorylation primes ISGylation); RBM47-ISGylation regulates TSC22D3 mRNA expression, and K329R knockin mice lacking RBM47-ISGylation show impaired immune activation and increased susceptibility to lung injury and tumorigenesis. K329R knockin mouse model, nanobody-targeted E3 ligase for site-specific ISGylation, RNA-seq, functional immune assays Cell death discovery High 38036512
2024 OTUD4 directly interacts with RBM47 and promotes its stability via deubiquitination; stabilized RBM47 then enhances ATF3 mRNA stability, promoting ATF3-mediated ferroptosis in clear cell renal cell carcinoma. Co-immunoprecipitation (OTUD4-RBM47 interaction), deubiquitination assay, mRNA stability assay, rescue experiments Apoptosis Medium 38553613
2021 RBM47 knockdown in mESCs perturbs primitive endoderm marker expression and increases GATA4+ PrE-like cells; this misregulation is reversed by FGFR or MEK inhibitors, implicating RBM47 in regulating FGF-ERK signaling during differentiation. RBM47 is also required for proper neuroectodermal and endodermal differentiation. siRNA knockdown in mouse ESCs, FGFR/MEK inhibitor rescue, lineage-specific differentiation assays, teratoma assays Stem cell reviews and reports Medium 35986129
2023 RBM47 binds to flanking introns of circFNDC3B pre-mRNA to facilitate its biogenesis, resulting in reduced linear FNDC3B mRNA levels and a circFNDC3B/FNDC3B mRNA imbalance in osteosarcoma. RIP assay, RNA-FISH, RNA stability analysis, functional assays Cancer cell international Low 38129874
2024 RBM47 stabilizes inflammatory gene mRNAs and activates the NF-κB pathway to promote M2 macrophage polarization in GBM; NF-κB in turn acts as a transcription factor to enhance RBM47 transcriptional activity, forming a positive feedback loop. RIP and CHIP assays confirmed these interactions. RNA-seq, RNA immunoprecipitation (RIP), chromatin immunoprecipitation (CHIP), immunofluorescence, in vitro and in vivo functional assays Phytomedicine Medium 38518642
2021 RBM47 inhibits K562 cell proliferation by binding HMGA2 mRNA and promoting its degradation, thereby reducing HMGA2 protein expression; overexpression of RBM47 decreases HMGA2 expression and inhibits cell cycle progression. RNA immunoprecipitation (RIP), mRNA stability assay (RT-qPCR with actinomycin D), Western blot, CCK-8/flow cytometry Zhongguo shi yan xue ye xue za zhi Low 34105460
2022 RBM47 binds to PTEN mRNA and stabilizes it, thereby suppressing PI3K/AKT signaling in colorectal cancer; miR-181c/d-5p directly targets RBM47 to downregulate it and reactivate PI3K/AKT signaling. RNA pulldown, luciferase reporter assay, RNA immunoprecipitation (RIP), mRNA stability assay, rescue experiments Molecular carcinogenesis Medium 36321407
2025 RBM47 directly binds the 3'UTR of cGAS mRNA via its RRM2 domain to enhance cGAS mRNA stability, thereby promoting cGAS-STING signaling and neuronal ferroptosis in a microglial context; RUNX1 directly binds the Rbm47 promoter to facilitate its transcription. RIP assay (RRM2 domain), promoter binding (ChIP implied), cGAS inhibitor epistasis, KD/OE functional assays in cells and mice Communications biology Medium 41407921
2025 RBM47 binds the 3'UTR of GSDMA mRNA and stabilizes it; ectopic RBM47 increases GSDMA mRNA and protein, while RBM47 knockdown reduces GSDMA. GSDMA is necessary for RBM47-induced mesenchymal-to-epithelial transition (MET), suppression of migration/invasion, and pyroptosis in colorectal cancer cells. RNA-Seq, RIP assay (direct 3'UTR binding), mRNA stability, GSDMA rescue/knockdown epistasis, MET and pyroptosis functional assays Cancers Medium 41681975
2025 RBM47 was identified as the trans-acting splicing factor that regulates tissue-specific alternative splicing of SEC31A (inclusion of a specific exon in digestive tissues); RBM47-regulated SEC31A splicing controls lipid transport (secretion of large cargo). Correlation of SEC31A alternative splicing with RBM47 expression across tissues was validated experimentally. RNA-seq across human tissues, in silico correlation of splicing with RBP expression, experimental validation of RBM47-mediated SEC31A splicing, lipid transport functional assay RNA (New York, N.Y.) Medium 40436629
2025 RBM47 stabilizes NEDD4L mRNA by binding to it, leading to increased NEDD4L-mediated ubiquitination and degradation of TRAF2, thereby promoting cellular injury and atherosclerotic plaque instability; silencing RBM47 reduced NEDD4L expression and attenuated TRAF2 ubiquitination. RIP assay, mRNA stability assay, NEDD4L-TRAF2 interaction confirmation, functional plaque stability assays in murine and cellular AS models International immunopharmacology Low 42177907
2025 HDAC2 promotes RBM47 expression through H3K27 deacetylation; RBM47 overexpression increases NONO expression in medulloblastoma cells, defining a HDAC2/RBM47/NONO axis in MB tumorigenesis. ChIP-qPCR, luciferase reporter assay, qRT-PCR, Western blot, KD functional assays Translational oncology Low 41027284
2025 RBM47 destabilizes ITGAV (integrin αV) mRNA by binding to AU-rich elements in its 3'UTR; miR-122 suppresses RBM47 expression, thereby elevating ITGAV, which activates latent TGF-β and promotes TGF-β signaling and HCC cell metastasis. Luciferase reporter, RIP assay (RBM47-ITGAV 3'UTR), mRNA stability assay, functional migration/invasion and in vivo pulmonary metastasis assays PloS one Medium 40638600
2026 RBM47 enhances ENC1 mRNA stability by binding to AU-rich elements in ENC1 mRNA, thereby curbing NRF2 synthesis and nuclear translocation in macrophages during atherosclerosis. RIP assay (AU-rich element binding), mRNA stability assay, macrophage-specific AAV-mediated knockdown in vivo, ENC1/RBM47 rescue experiments Biochemical pharmacology Medium 41962778
2025 METTL3/YTHDF1-dependent m6A methylation decreases RBM47 expression in NSCLC; RBM47 destabilizes PD-L1 mRNA via binding its 3'UTR, thereby enhancing T-cell-mediated antitumor activity. Methylated RIP-PCR (m6A modification), RIP assay (PD-L1 3'UTR binding), functional T-cell cytotoxicity assays, in vitro and in vivo cancer stem cell assays Journal of thoracic disease Low 41376900
2023 RBM47 binds to and stabilizes FBXO2 mRNA, promoting phosphorylation of STAT3 in chondrocytes, thereby advancing osteoarthritis development; RBM47 knockdown alleviates inflammation, apoptosis, and ECM degradation in IL-1β-treated chondrocytes. RIP assay, mRNA stability assay, STAT3 phosphorylation Western blot, FBXO2/STAT3 rescue experiments, in vitro chondrocyte model Biochemical genetics Low 38070024
2026 CCDC12 knockdown leads to increased exon 5 skipping in RBM47 pre-mRNA, producing a truncated RBM47 isoform with reduced tumor-suppressive activity; reintroduction of full-length RBM47 partially restores impaired cellular phenotypes in breast cancer. Transcriptomic analysis, splicing assays, RBM47 full-length rescue experiments, functional proliferation/metastasis assays International journal of biological macromolecules Low 41690343
2025 FOXA1 directly binds FOXA1 binding sites within the RBM47 promoter region to activate RBM47 transcription; RBM47 upregulation is necessary for FOXA1-mediated mesenchymal-to-epithelial transition and inhibition of CRC cell migration. RBM47 promoter CpG methylation silences RBM47 in mesenchymal-like CRC cells. ChIP (FOXA1 binding to RBM47 promoter), ectopic FOXA1 expression, RBM47 rescue experiments, bisulfite sequencing/methylation analysis Molecular biomedicine Medium 41335176
2023 Intestine-specific Rbm47 knockout mice exhibit increased proliferation and abnormal villus morphology; these mice show upregulation of antioxidant, Wnt signaling pathways, stem cell and developmental genes, are protected against colitis but develop spontaneous polyposis with age; Rbm47-IKO ApcMin/+ mice show increased polyp burden. Alternative splicing of TJP1 mRNA is altered in human CRC consistent with loss of RBM47. Intestine-specific conditional knockout mice (Rbm47-IKO), organoid assays, radiation/chemical colitis models, ApcMin/+ cross, RNA-seq for splicing and expression changes JCI insight High 37014710
2024 RBM47 binds the 3'UTR of PDIA6 mRNA and maintains its stability, increasing PDIA6 expression in pancreatic cancer cells; PDIA6 overexpression reverses the suppressive effects of RBM47 knockdown on cell proliferation and immune evasion, establishing RBM47-PDIA6 as a functional axis. RNA immunoprecipitation (RIP), dual luciferase reporter assay, mRNA stability assay, rescue experiments, xenograft model Journal of translational medicine Medium 39741300
2025 RBM47 directly interacts with HOXB-AS1 lncRNA, interfering with the HOXB-AS1–p53 protein interaction; this promotes p53 nuclear entry and activation of p53 signaling in renal cell carcinoma cells. Co-immunoprecipitation (RBM47-HOXB-AS1 interaction), p53 nuclear localization assay, functional knockdown/rescue experiments Cell death discovery Low 37660095

Source papers

Stage 0 corpus · 47 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Loss of the multifunctional RNA-binding protein RBM47 as a source of selectable metastatic traits in breast cancer. eLife 122 24898756
2014 C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO reports 94 24916387
2017 Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression. Scientific reports 80 28680090
2022 RBM47/SNHG5/FOXO3 axis activates autophagy and inhibits cell proliferation in papillary thyroid carcinoma. Cell death & disease 65 35338124
2020 The RNA-binding protein RBM47 inhibits non-small cell lung carcinoma metastasis through modulation of AXIN1 mRNA stability and Wnt/β-catentin signaling. Surgical oncology 46 32891348
2019 RBM47-regulated alternative splicing of TJP1 promotes actin stress fiber assembly during epithelial-to-mesenchymal transition. Oncogene 45 31358901
2019 The RNA-binding protein RBM47 is a novel regulator of cell fate decisions by transcriptionally controlling the p53-p21-axis. Cell death and differentiation 42 31511650
2018 Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver. RNA (New York, N.Y.) 42 30309881
2021 RNA binding motif 47 (RBM47): emerging roles in vertebrate development, RNA editing and cancer. Molecular and cellular biochemistry 32 34499322
2018 Post-transcriptional regulator Rbm47 elevates IL-10 production and promotes the immunosuppression of B cells. Cellular & molecular immunology 32 29844590
2021 RNA-binding protein RBM47 stabilizes IFNAR1 mRNA to potentiate host antiviral activity. EMBO reports 27 34160127
2021 RNA-binding motif protein RBM47 promotes tumorigenesis in nasopharyngeal carcinoma through multiple pathways. Journal of genetics and genomics = Yi chuan xue bao 27 34274258
2013 rbm47, a novel RNA binding protein, regulates zebrafish head development. Developmental dynamics : an official publication of the American Association of Anatomists 26 24038582
2021 Identifying RBM47, HCK, CD53, TYROBP, and HAVCR2 as Hub Genes in Advanced Atherosclerotic Plaques by Network-Based Analysis and Validation. Frontiers in genetics 24 33519905
2019 Comparison of RNA Editing Activity of APOBEC1-A1CF and APOBEC1-RBM47 Complexes Reconstituted in HEK293T Cells. Journal of molecular biology 23 30844405
2022 RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator. Cell death discovery 21 35831298
2023 RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways. JCI insight 20 37014710
2020 Zebrafish RBM47 Promotes Lysosome-Dependent Degradation of MAVS to Inhibit IFN Induction. Journal of immunology (Baltimore, Md. : 1950) 18 32859727
2016 Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth. Genesis (New York, N.Y. : 2000) 17 26789794
2024 Dioscin decreases M2 polarization via inhibiting a positive feedback loop between RBM47 and NF-κB in glioma. Phytomedicine : international journal of phytotherapy and phytopharmacology 12 38518642
2024 Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells. Apoptosis : an international journal on programmed cell death 11 38553613
2022 Saponin from Platycodi radix inactivates PI3K/AKT signaling pathway to hinder colorectal cancer cell proliferation, invasion, and migration through miR-181c/d-5p/RBM47. Molecular carcinogenesis 10 36321407
2013 RNA-binding protein Rbm47 binds to Nanog in mouse embryonic stem cells. Molecular biology reports 10 23649762
2023 Dynamic RBM47 ISGylation confers broad immunoprotection against lung injury and tumorigenesis via TSC22D3 downregulation. Cell death discovery 8 38036512
2023 A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma. Cancer cell international 8 38129874
2024 Copy number amplification-induced overexpression of lncRNA LOC101927668 facilitates colorectal cancer progression by recruiting hnRNPD to disrupt RBM47/p53/p21 signaling. Journal of experimental & clinical cancer research : CR 6 39350250
2023 RBM47 restrains renal cell carcinoma progression and chemoresistance through interacting with lncRNA HOXB-AS1. Cell death discovery 6 37660095
2022 RBM47 is a Critical Regulator of Mouse Embryonic Stem Cell Differentiation. Stem cell reviews and reports 6 35986129
2024 RBM47 promotes cell proliferation and immune evasion by upregulating PDIA6: a novel mechanism of pancreatic cancer progression. Journal of translational medicine 5 39741300
2023 Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics 5 38070024
2025 MiR-122 promotes metastasis of hepatoma cells by modulating RBM47-integrin alpha V-TGF-beta signaling. PloS one 3 40638600
2025 Down-regulation of RBM47 due to diminished activation by forkhead box A1 (FOXA1) and silencing by CpG methylation is associated with epithelial-mesenchymal transition and metastasis of colorectal cancer. Molecular biomedicine 3 41335176
2023 Changes in RBM47 expression based on the timing of melatonin administration and its effects on Nrf2 activity in the hippocampus. Free radical biology & medicine 3 37751802
2023 RNA-Binding Motif Protein RBM47 Promotes Invasiveness of Glioblastoma Through Activation of Epithelial-to-Mesenchymal Transition Program. Genetic testing and molecular biomarkers 3 38156907
2025 RBM47 functions as an anti-oncogene by regulating expression and alternative splicing of cell proliferation and apoptosis associated genes in colorectal cancer cells. Scientific reports 2 40695891
2025 RBM47 as a potential therapeutic target for thyroid-associated ophthalmopathy. International immunopharmacology 1 39746275
2025 HDAC2 promotes malignant progression by the RBM47/NONO axis in medulloblastoma. Translational oncology 1 41027284
2025 Pseudomonas putida KT2440-induced RBM47 regulates non-small cell lung cancer stem cell properties and T cell-mediated antitumor activity. Journal of thoracic disease 1 41376900
2025 Microglial RUNX1/RBM47 ablation inhibits neuronal ferroptosis via regulating the cGAS-STING-MEF2C pathway in mice with postoperative cognitive dysfunction. Communications biology 1 41407921
2025 The role of positive feedback loop between LINC00862 and RBM47 in hepatocellular carcinoma suppression. Genes & diseases 1 41487470
2021 [RNA Binding Protein RBM47 Inhibits the K562 Cell Proliferation by Regulating HMGA2 mRNA Expression]. Zhongguo shi yan xue ye xue za zhi 1 34105460
2026 RBM47-Induced Gasdermin A/GSDMA Mediates Mesenchymal-Epithelial Transition and Pyroptosis of Colorectal Cancer Cells. Cancers 0 41681975
2026 Unveiling the oncogenic function of CCDC12 in regulating RBM47 splicing in breast cancer. International journal of biological macromolecules 0 41690343
2026 RNA-binding protein RBM47 enhances ENC1 stability through AU-rich elements to induce oxidative stress in macrophages in atherosclerosis progression. Biochemical pharmacology 0 41962778
2026 Dexmedetomidine Attenuates Neonatal Hypoxic-Ischemic Encephalopathy by Inhibiting Microglia Activation Through Upregulating Rbm47. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 42065818
2026 RBM47 aggravates carotid atherosclerotic plaque instability through NEDD4L-dependent regulation of TRAF2. International immunopharmacology 0 42177907
2025 Tissue-specific SEC31A alternative splicing is regulated by RBM47 and controls lipid transport. RNA (New York, N.Y.) 0 40436629

Missed literature

Know a paper Affinage missed for RBM47? Flag it for the maintainers and the community.

No submissions yet.