Affinage

RBM15

RNA-binding protein 15 · UniProt Q96T37

Length
977 aa
Mass
107.2 kDa
Annotated
2026-06-10
100 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBM15 (OTT1) is a spen-family RNA-binding protein with N-terminal RRM domains and a C-terminal SPOC domain that functions as the mRNA-binding adaptor of the m6A methyltransferase writer complex, coupling RNA recognition to post-transcriptional and epigenetic regulation in hematopoietic and developmental contexts (PMID:11431691, PMID:29535189). As an adaptor, RBM15 bridges target mRNAs to the WTAP/METTL3 machinery — a role established genetically through ZC3H13/Flacc-mediated linkage of Rbm15/Nito to the WTAP component (PMID:29535189) — and directs m6A deposition onto specific transcripts to control their fate, including degradation of BAF155 mRNA in a METTL3-dependent manner (PMID:31020615) and stabilization of STYK1 mRNA driving MAPK activation (PMID:36147665). Beyond methylation, RBM15 regulates alternative splicing by binding pre-mRNA introns of megakaryopoietic genes (GATA1, RUNX1, TAL1, c-MPL) and recruiting SF3B1, and it generates a dominant-negative c-Mpl isoform while modifying H3K4me3 and H4 acetylation at the c-Mpl locus together with Setd1b (KMT2G) and HDAC3 (PMID:25468569, PMID:26575292). It also acts at the nuclear envelope as an NXF1 cofactor that bridges mRNPs to the DBP5 helicase to drive efficient mRNA nuclear export (PMID:19786495). RBM15 protein abundance is tightly controlled: PRMT1 methylates R578 to trigger CNOT4-dependent ubiquitin degradation (PMID:26575292), whereas lactylation at K850 stabilizes the protein, is reversed by HDAC3, and is required for the RBM15-METTL3 association (PMID:40135634). Physiologically, RBM15 is essential for B lymphopoiesis and restrains myeloid/megakaryocytic expansion and HSC numbers (PMID:17376872), required for placental vascular branching morphogenesis during embryogenesis (PMID:18981216), and necessary for Xist-mediated X chromosome silencing through recruitment to the Xist A-repeat and 5' m6A region (PMID:26190105, PMID:32258426). The recurrent t(1;22) RBM15-MKL1 fusion of acute megakaryoblastic leukemia deregulates RBPJ/Notch transcription and, retaining RNA-binding and m6A functions, redirects the writer complex onto distinct targets including Wnt-pathway Frizzled mRNAs, conferring METTL3-inhibitor sensitivity (PMID:19287095, PMID:40435410).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2001 High

    Establishing that RBM15 is an RRM-containing spen-family protein recurrently fused to MKL1 in t(1;22) acute megakaryoblastic leukemia first linked the gene to RNA binding and to leukemogenesis.

    Evidence Molecular cloning and sequencing of translocation breakpoints

    PMID:11431691

    Open questions at the time
    • Did not define the molecular function of the RRM domains
    • Mechanism by which the fusion transforms cells unknown
  2. 2007 High

    Conditional deletion in mice defined RBM15 as physiologically required for B lymphopoiesis while restraining myeloid/megakaryocytic lineages and the HSC compartment, anchoring its role in hematopoietic differentiation.

    Evidence Conditional knockout mouse, flow cytometry, bone marrow analysis

    PMID:17376872

    Open questions at the time
    • Molecular targets driving lineage skewing not identified
    • Did not connect phenotype to a specific biochemical activity
  3. 2007 Medium

    Identification of RBPJkappa co-immunoprecipitation and cell-type-specific modulation of Notch-induced HES1 transcription connected RBM15 to a defined signaling transcription factor.

    Evidence RNAi, enforced expression, co-IP, luciferase reporters

    PMID:17283045

    Open questions at the time
    • Basis of opposite effects in hematopoietic vs non-hematopoietic cells unresolved
    • Direct vs indirect RBPJkappa contact not mapped
  4. 2008 High

    A trophoblast-sparing genetic rescue established a tissue-specific developmental requirement, showing RBM15 is essential for placental vascular branching morphogenesis and embryonic survival.

    Evidence Germline/conditional KO with conditional rescue and expression profiling

    PMID:18981216

    Open questions at the time
    • Downstream effectors of the vascular defect not defined
    • Link to RNA-level mechanism absent
  5. 2009 High

    Reciprocal binding and knockdown experiments placed RBM15 at the nuclear pore as an NXF1 cofactor that bridges mRNPs to the DBP5 helicase, defining a direct role in mRNA nuclear export.

    Evidence Co-IP, RIP, siRNA knockdown, fractionation, immunofluorescence

    PMID:19786495

    Open questions at the time
    • RNA selectivity of export function not delineated
    • Relationship between export and later m6A roles unaddressed
  6. 2009 High

    A knockin model demonstrated that the RBM15-MKL1 fusion deregulates RBPJ-dependent Notch transcription and cooperates with MPL mutation to drive AMKL, mechanistically tying the fusion to malignant megakaryopoiesis.

    Evidence Knockin mouse, reporter assays, genetic cooperation with MPL

    PMID:19287095

    Open questions at the time
    • RNA-binding contribution of the fusion not yet examined
    • Direct fusion targets not catalogued
  7. 2012 Medium

    Domain mapping showed the RBM15 SPOC domain directly binds the Setd1b (KMT2G) H3K4 methyltransferase and that this interaction is required for cytokine-independent AMKL proliferation, linking RBM15 to chromatin modification.

    Evidence Co-IP, domain mapping, growth assays with SPOC mutants

    PMID:22927943

    Open questions at the time
    • Genomic loci of Setd1b recruitment not defined here
    • Single-lab interaction
  8. 2014 High

    Coupling RNA binding to chromatin marks, RBM15 was shown to control c-Mpl alternative splicing into a dominant-negative isoform while binding c-Mpl RNA and chromatin and regulating H3K4me3/H4 acetylation with Hdac3 and Setd1b.

    Evidence Conditional KO, splicing analysis, ChIP, RIP, histone modification assays

    PMID:25468569

    Open questions at the time
    • Generality of RNA+chromatin coupling beyond c-Mpl untested
    • Splicing machinery recruited not identified in this study
  9. 2015 High

    RBM15 was shown to bind pre-mRNA introns of megakaryopoietic genes and recruit SF3B1, and its abundance to be controlled by PRMT1 methylation at R578 driving CNOT4-dependent degradation, defining both a splicing mechanism and a regulatory degradation axis.

    Evidence In vitro methylation/ubiquitylation assays, RIP, MS, R578 mutagenesis, rescue in AMKL lines

    PMID:26575292

    Open questions at the time
    • How SF3B1 recruitment alters splice site choice not resolved
    • PRMT1 regulation outside megakaryopoiesis untested
  10. 2015 Medium

    An shRNA screen and super-resolution imaging identified RBM15 as required for Xist-mediated silencing and co-localizing with Xist RNA in the nuclear matrix, extending its function to X chromosome inactivation.

    Evidence shRNA screen, knockdown validation, 3D-SIM, allelic RNA-seq

    PMID:26190105

    Open questions at the time
    • Mechanistic step in silencing not defined at this stage
    • Direct Xist contact not yet mapped
  11. 2016 Medium

    Discovery that the antisense AS-RBM15 lncRNA enhances cap-dependent RBM15 translation added a layer of RBM15 protein-level control independent of degradation.

    Evidence Overexpression/knockdown of AS-RBM15, translation reporters, domain mapping

    PMID:27118388

    Open questions at the time
    • Physiological contexts where AS-RBM15 operates unknown
    • Single-lab finding
  12. 2018 High

    Cross-species co-IP and genetic epistasis established RBM15 as the mRNA-binding adaptor that links target transcripts to the WTAP/METTL3 m6A writer complex via ZC3H13/Flacc, redefining its core molecular identity.

    Evidence Reciprocal co-IP in Drosophila and mouse, m6A quantification, sex-determination epistasis

    PMID:29535189

    Open questions at the time
    • Sequence determinants of RBM15 target selection not defined
    • How adaptor role integrates with export/splicing roles unclear
  13. 2020 High

    CRISPR deletion of the Xist A-repeat plus interactome MS showed RBM15 is recruited to the Xist A-repeat to direct 5' m6A deposition and partners with both the m6A and SETD1B complexes, mechanistically uniting its RNA-methylation and chromatin roles at Xist.

    Evidence CRISPR A-repeat deletion, m6A-seq, allelic RNA-seq, epitope-tag MS/MS

    PMID:32258426

    Open questions at the time
    • Causal contribution of 5' m6A to silencing not isolated
    • Stoichiometry of dual complex association unknown
  14. 2019 Medium

    RBM15 was shown to bind BAF155 mRNA and drive its METTL3-dependent degradation, controlling cortical radial glial progenitor delamination and demonstrating target-specific m6A-mediated decay in neural development.

    Evidence RIP, CRISPR/shRNA in vitro and in vivo, overexpression, METTL3 dependence

    PMID:31020615

    Open questions at the time
    • Single target focus; broader neural m6A program undefined
    • Recruitment specificity to BAF155 not mapped
  15. 2020 Medium

    A zebrafish forward-genetic screen revealed RBM15 is required specifically for liver maturation via restraint of mTORC1 and proper Hnf4a nuclear localization, broadening its developmental role beyond hematopoiesis and placenta.

    Evidence Forward screen, positional cloning, CRISPR KO, rapamycin rescue

    PMID:32518161

    Open questions at the time
    • Molecular link between RBM15 and mTORC1 unknown
    • Whether effect is m6A-dependent untested
  16. 2022 Medium

    In vitro reconstitution showed RBM15 forms RNA/protein-concentration-dependent liquid-like nuclear condensates that co-localize with m6A transcripts and promotes m6A on STYK1 mRNA to drive oncogenic MAPK activation, adding a biophysical organizing principle to its writer-adaptor function.

    Evidence SIM imaging, in vitro phase separation, MeRIP-seq, RNA-seq, NIH3T3 transformation

    PMID:36147665

    Open questions at the time
    • In vivo functional requirement of condensates not established
    • Determinants of STYK1 selectivity unclear
  17. 2025 Medium

    Identification of MCT1/lactate-driven K850 lactylation that stabilizes RBM15 and is reversed by HDAC3 — and is required for RBM15-METTL3 association — defined a metabolic input controlling RBM15 abundance and m6A output.

    Evidence MS lactylation mapping, HDAC3 delactylase assays, K850R mutagenesis, co-IP, ubiquitination/m6A assays

    PMID:40135634

    Open questions at the time
    • Physiological/disease contexts of lactylation untested
    • Single-lab PTM characterization
  18. 2025 High

    Multi-omic and pharmacologic analyses showed the RBM15-MKL1 fusion retains RNA-binding/m6A activity but redirects it onto distinct targets including Wnt-pathway Frizzled mRNAs, conferring METTL3-inhibitor and Frizzled-knockdown vulnerability in AMKL.

    Evidence Transcriptome-wide RNA binding, m6A mapping, RNA turnover, METTL3 inhibitor, Frizzled knockdown, transplantation

    PMID:40435410

    Open questions at the time
    • How fusion alters target selection relative to wild-type not fully resolved
    • Generalizability across AMKL subtypes untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • The sequence and structural determinants by which RBM15 selects specific target mRNAs — and how its export, splicing, m6A-adaptor, and condensate functions are coordinated on a given transcript — remain unresolved.
  • No defined RNA sequence/structure code for RBM15 target choice
  • Integration of multiple molecular activities on single transcripts unmapped
  • Structural basis of SPOC-mediated complex assembly incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 4 GO:0005635 nuclear envelope 1
Pathway
R-HSA-8953854 Metabolism of RNA 4 R-HSA-1266738 Developmental Biology 3 R-HSA-1643685 Disease 3 R-HSA-74160 Gene expression (Transcription) 3
Partners
DBP5HDAC3METTL3NXF1SETD1BSF3B1WTAPZC3H13
Complex memberships
SETD1B (KMT2G) histone methyltransferase complexm6A methyltransferase (METTL3/WTAP) writer complex

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 RBM15 (OTT1) was identified as a novel gene encoding an RNA recognition motif (RRM)-containing protein with homology to Drosophila spen, fused to MKL1 in the t(1;22)(p13;q13) translocation of acute megakaryoblastic leukemia. Molecular cloning and sequencing of translocation breakpoints Nature genetics High 11431691
2002 The OTT-MAL (RBM15-MKL1) fusion gene is present in all t(1;22) acute megakaryoblastic leukemia cases studied, with the translocation occurring via a non-homologous end joining mechanism involving a non-canonical topoisomerase II-like consensus sequence in the OTT gene. Nucleotide sequence analysis of translocation breakpoints, FISH, RT-PCR Genes, chromosomes & cancer Medium 11746984
2005 RBM15 (OTT1) interacts via its SPOC domain with the Epstein-Barr virus mRNA export factor EB2, and this interaction is mechanistically linked to splicing regulation; OTT1 and SHARP SPOC domains interact with SMRT/NCoR corepressors but with far weaker repressive activity than SHARP. Yeast two-hybrid screen, co-immunoprecipitation, transcriptional repression assays The Journal of biological chemistry Medium 16129689
2007 RBM15 modulates Notch-induced HES1 promoter activity in a cell-type-specific manner: it inhibits Notch-induced HES1 transcription in non-hematopoietic cells but stimulates it in hematopoietic cell lines. The N-terminus of RBM15 co-immunoprecipitates with RBPJkappa, a critical Notch signaling transcription factor. RNA interference, enforced expression, co-immunoprecipitation, luciferase reporter assays Molecular and cellular biology Medium 17283045
2007 Conditional deletion of Ott1 (Rbm15) in adult mice causes a block in pro/pre-B differentiation leading to loss of peripheral B cells, myeloid and megakaryocytic expansion, and increased Lin-Sca-1+c-Kit+ hematopoietic stem cells, establishing Ott1 as required for B lymphopoiesis and inhibitory in myeloid/megakaryocytic lineages. Conditional knockout mouse model, flow cytometry, bone marrow analysis Proceedings of the National Academy of Sciences of the United States of America High 17376872
2008 OTT (RBM15) interacts with histone deacetylase 3 (HDAC3), but this interaction is abolished in the OTT-BSAC fusion protein. OTT-BSAC, unlike BSAC alone, exclusively localizes to the nucleus (BSAC normally resides predominantly in cytoplasm), activating YY1-binding sequence-containing promoters. Co-immunoprecipitation, subcellular fractionation/localization, luciferase reporter assays The Journal of biological chemistry Medium 18667423
2008 Germ-line deletion of Ott1 (Rbm15) causes embryonic lethality by E10.5 due to defects in placental vascular branching morphogenesis in spongiotrophoblast and syncytiotrophoblast layers. Trophoblast-specific rescue restores placenta and allows survival to term, revealing cardiac and splenic developmental abnormalities with enrichment of hypoxia-related gene expression changes. Germ-line and conditional knockout mouse models, conditional rescue with trophoblast-sparing Cre, global gene expression analysis Molecular and cellular biology High 18981216
2009 The OTT-MAL (RBM15-MKL1) fusion oncogene deregulates transcriptional activity of the Notch signaling pathway transcription factor RBPJ, causing abnormal fetal megakaryopoiesis; cooperation with activating MPL mutation efficiently induces short-latency AMKL in a knockin mouse model. Knockin mouse model, transcriptional reporter assays, genetic cooperation experiments with MPL mutation The Journal of clinical investigation High 19287095
2009 RBM15 binds specifically to the RNA helicase DBP5 and facilitates DBP5's direct contact with mRNA in vivo. RBM15 localizes to the nuclear envelope where it co-localizes with DBP5 and NXF1. Gene silencing of RBM15 leads to cytoplasmic depletion and nuclear accumulation of bulk mRNA and specific endogenous transcripts, establishing RBM15 as required for efficient mRNA nuclear export. Co-immunoprecipitation, RNA immunoprecipitation, siRNA knockdown, subcellular fractionation, immunofluorescence Nucleic acids research High 19786495
2010 RBM15 and OTT3 (RBM15B) function as cellular NXF1 cofactors; knockdown of RBM15 leads to nuclear export deficiency of KSHV ORF57 RNA. KSHV ORF57 interacts directly with the RBM15 C-terminal SPOC domain to reduce RBM15 binding to ORF59 RNA. RNAi knockdown, RNA FISH, co-immunoprecipitation, subcellular RNA fractionation Journal of virology Medium 21106733
2012 RBM15 and the leukemogenic RBM15-MKL1 fusion protein directly interact with the Setd1b histone H3-Lys4 methyltransferase (KMT2G) via the RBM15 SPOC domain and the Setd1b LSD motif. Cytokine-independent proliferation of AMKL cells requires an intact RBM15 SPOC domain mediating this interaction. Co-immunoprecipitation, domain-mapping experiments, cytokine-independent growth assays with SPOC domain mutants PloS one Medium 22927943
2014 Ott1 (Rbm15) controls alternative splicing of c-Mpl to generate a dominant-negative isoform (Mpl-TR) that inhibits HSC engraftment and attenuates Thpo signaling. Ott1 associates with Hdac3 and the histone methyltransferase Setd1b, binds both c-Mpl RNA and chromatin, and regulates H4 acetylation and H3K4me3 marks at the c-Mpl locus. Conditional KO mouse model, alternative splicing analysis, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), histone modification assays Blood High 25468569
2015 RBM15 is methylated by PRMT1 at residue R578, which leads to its ubiquitin-mediated degradation via the E3 ligase CNOT4. RBM15 binds to pre-mRNA intronic regions of megakaryopoiesis-related genes (GATA1, RUNX1, TAL1, c-MPL) and recruits the splicing factor SF3B1 to the same intronic sites, regulating alternative splicing. PRMT1 overexpression blocks megakaryocyte differentiation by downregulating RBM15 protein; restoring RBM15 rescues differentiation. In vitro methylation assay, ubiquitylation assay, RNA immunoprecipitation (RIP), mass spectrometry, site-directed mutagenesis (R578), rescue experiments in AMKL cell lines eLife High 26575292
2015 A pooled shRNA screen identified RBM15 as required for Xist RNA-mediated silencing in X chromosome inactivation. RBM15 co-localizes with Xist RNA within the nuclear matrix subcompartment as shown by super-resolution 3D-SIM microscopy. shRNA screen, validation by knockdown, super-resolution 3D-SIM immunofluorescence microscopy, allelic RNA-seq Cell reports Medium 26190105
2016 AS-RBM15, an antisense RNA transcribed from within exon 1 of RBM15, enhances RBM15 protein translation in a CAP-dependent manner. The region of AS-RBM15 overlapping with the 5'UTR of RBM15 is sufficient for upregulation of RBM15 protein translation. Overexpression and knockdown of AS-RBM15, translation reporter assays, domain mapping EMBO reports Medium 27118388
2018 Zc3h13/Flacc promotes m6A deposition by bridging the WTAP/Fl(2)d component of the m6A methyltransferase complex to the mRNA-binding factor Rbm15/Nito (Spenito), establishing RBM15 as the mRNA-binding adaptor that links target mRNAs to the METTL3 writer complex. Co-immunoprecipitation in Drosophila and mouse, m6A quantification, genetic analysis of sex determination pathway (epistasis), protein interaction mapping Genes & development High 29535189
2019 RBM15, as a subunit of the m6A methyltransferase complex, interacts with BAF155 mRNA and mediates its degradation through the mRNA methylation machinery in a METTL3-dependent manner. RBM15 ablation in neuronal cells and developing cortex increases BAF155 expression; RBM15 overexpression decreases BAF155 mRNA/protein and perturbs BAF155-dependent transcriptional activity and apical radial glial progenitor delamination. RIP assay, CRISPR/shRNA loss-of-function in vitro and in vivo, overexpression experiments, m6A pathway functional dependence on METTL3 Molecular neurobiology Medium 31020615
2020 RBM15 binds to the Xist A-repeat region; deletion of the Xist A-repeat entirely abolishes m6A deposition in the Xist 5' m6A region without affecting exon VII m6A. In mESCs, RBM15 interacts with the m6A methyltransferase complex and with the SETD1B histone-modifying complex, plus several RNA metabolism proteins, as determined by epitope-tag purification and MS/MS. CRISPR/Cas9 deletion of Xist A-repeat, m6A-seq, allelic RNA-seq, epitope-tag purification followed by MS/MS interactome analysis Wellcome open research High 32258426
2020 Loss of Rbm15 in zebrafish (cq96 mutant identified by forward genetic screen) causes a specific defect in liver maturation without affecting hepatoblast specification, differentiation, or hepatocyte proliferation/apoptosis. The mTORC1 pathway is hyperactivated in rbm15-deficient hepatocytes, and rapamycin treatment partially restores normal hepatic gene expression and nuclear localization of the transcription factor Hnf4a. Forward genetic screen, positional cloning, CRISPR/Cas9 knockout in zebrafish, rapamycin rescue, mTORC1 pathway analysis The Journal of biological chemistry Medium 32518161
2022 RBM15 forms liquid-like phase-separated condensates (droplets) in the nucleus in a protein/salt/RNA concentration-dependent manner, with condensates partially co-localizing with m6A-modified transcripts. RBM15 preferentially binds to and promotes m6A modification on STYK1 mRNA, enhancing its stability and leading to MAPK hyperactivation and oncogenic transformation of NIH3T3 cells. Super-resolution structured illumination microscopy (SIM), in vitro phase separation assays, MeRIP-seq, RNA-seq, NIH3T3 transformation assay Computational and structural biotechnology journal Medium 36147665
2025 L-lactate promotes lactylation of RBM15 at Lys850 (K850), mediated by MCT1-dependent lactate uptake. Lactylation at K850 stabilizes RBM15 by inhibiting proteasome-mediated ubiquitin degradation. HDAC3 acts as the delactylase for RBM15. K850R mutation disrupts the association between RBM15 and METTL3 and reduces global m6A levels, abrogating RBM15-mediated cell proliferation and migration. Mass spectrometry identification of lactylation sites, HDAC3 delactylase identification, K850R site-directed mutagenesis, co-immunoprecipitation (RBM15-METTL3), ubiquitination assay, m6A quantification FASEB journal Medium 40135634
2025 The RBM15-MKL1 fusion protein retains the RNA-binding and m6A-modifying functions of RBM15 while selectively regulating distinct mRNA targets including Frizzled genes in the Wnt signaling pathway. METTL3 inhibition (STM3675) decreases m6A deposition, induces apoptosis in RM-AMKL cells in vitro, and prolongs survival in transplanted mice. Direct Frizzled knockdown reduces RM-AMKL growth in vitro and in vivo. Multi-omics (transcriptome-wide RNA binding, m6A mapping, RNA turnover), METTL3 inhibitor treatment, Frizzled knockdown, in vivo transplantation model Blood High 40435410
2025 RBM15 promotes m6A modification of SRSF1 mRNA by recruiting YTHDF3, increasing SRSF1 stability. Elevated SRSF1 then promotes ATP7B exon 21 alternative splicing, inhibiting cuproptosis and promoting NSCLC cell proliferation. RIP assay, MeRIP assay, xenograft tumor model, alternative splicing analysis, shRNA knockdown The Kaohsiung journal of medical sciences Low 40923717

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes & development 514 29535189
2001 Fusion of two novel genes, RBM15 and MKL1, in the t(1;22)(p13;q13) of acute megakaryoblastic leukemia. Nature genetics 240 11431691
2015 A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing. Cell reports 229 26190105
2021 RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. Journal of experimental & clinical cancer research : CR 207 33637103
2015 Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. eLife 160 26575292
2009 The OTT-MAL fusion oncogene activates RBPJ-mediated transcription and induces acute megakaryoblastic leukemia in a knockin mouse model. The Journal of clinical investigation 95 19287095
2007 Rbm15 modulates Notch-induced transcriptional activation and affects myeloid differentiation. Molecular and cellular biology 89 17283045
2005 Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. The Journal of biological chemistry 82 16129689
2007 Ott1(Rbm15) has pleiotropic roles in hematopoietic development. Proceedings of the National Academy of Sciences of the United States of America 79 17376872
2016 The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation. EMBO reports 76 27118388
2022 Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell proliferation 74 36181462
2021 RBM15 promotes hepatocellular carcinoma progression by regulating N6-methyladenosine modification of YES1 mRNA in an IGF2BP1-dependent manner. Cell death discovery 71 34707107
2009 Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Nucleic acids research 55 19786495
2022 Epigenetic activation of RBM15 promotes clear cell renal cell carcinoma growth, metastasis and macrophage infiltration by regulating the m6A modification of CXCL11. Free radical biology & medicine 53 35381326
2021 Knockdown RBM15 Inhibits Colorectal Cancer Cell Proliferation and Metastasis Via N6-Methyladenosine (m6A) Modification of MyD88 mRNA. Cancer biotherapy & radiopharmaceuticals 49 34842457
2019 RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex. Molecular neurobiology 49 31020615
2008 Ott1 (Rbm15) is essential for placental vascular branching morphogenesis and embryonic development of the heart and spleen. Molecular and cellular biology 46 18981216
2010 Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59. Journal of virology 45 21106733
2023 AZGP1P2/UBA1/RBM15 Cascade Mediates the Fate Determinations of Prostate Cancer Stem Cells and Promotes Therapeutic Effect of Docetaxel in Castration-Resistant Prostate Cancer via TPM1 m6A Modification. Research (Washington, D.C.) 44 37854295
2020 The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation. Wellcome open research 44 32258426
2014 Ott1 (Rbm15) regulates thrombopoietin response in hematopoietic stem cells through alternative splicing of c-Mpl. Blood 43 25468569
2012 Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation. PloS one 42 22927943
2002 Recurrence of OTT-MAL fusion in t(1;22) of infant AML-M7. Genes, chromosomes & cancer 42 11746984
2024 The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism. Experimental & molecular medicine 39 38825643
2023 RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4. Molecular medicine (Cambridge, Mass.) 35 36803098
2021 RBM15-mediated N6-methyladenosine modification affects COVID-19 severity by regulating the expression of multitarget genes. Cell death & disease 34 34301919
2018 MED12, TERT promoter and RBM15 mutations in primary and recurrent phyllodes tumours. British journal of cancer 33 29315289
2012 Hematopoietic stem cells lacking Ott1 display aspects associated with aging and are unable to maintain quiescence during proliferative stress. Blood 33 22490678
2023 RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines. Cancers 32 36831430
2023 Knockdown of RBM15 inhibits tumor progression and the JAK-STAT signaling pathway in cervical cancer. BMC cancer 31 37474926
2023 RBM15 silencing promotes ferroptosis by regulating the TGF-β/Smad2 pathway in lung cancer. Environmental toxicology 30 36715115
2016 RBM15 Functions in Blood Diseases. Current cancer drug targets 30 26758534
2023 RBM15 m6 A modification-mediated OTUB2 upregulation promotes cervical cancer progression via the AKT/mTOR signaling. Environmental toxicology 29 37334762
2012 Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells. Leukemia & lymphoma 27 22497198
2023 RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer. International journal of oncology 26 37594126
2023 N6-methyladenosine methylation regulator RBM15 promotes the progression of diabetic nephropathy by regulating cell proliferation, inflammation, oxidative stress, and pyroptosis through activating the AGE-RAGE pathway. Environmental toxicology 25 37551785
2024 RBM15 facilitates osimertinib resistance of lung adenocarcinoma through m6A-dependent epigenetic silencing of SPOCK1. Oncogene 23 39528815
1996 Ott, a mouse X-linked multigene family expressed specifically during meiosis. Human molecular genetics 23 8842733
2024 m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma. Molecular carcinogenesis 22 39092767
2015 Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15. Viruses 21 25690794
2023 HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition. Cell biology international 20 37191290
2018 PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Experimental and therapeutic medicine 19 29456659
2024 RBM15 facilities lung adenocarcinoma cell progression by regulating RASSF8 stability through N6 Methyladenosine modification. Translational oncology 17 38838436
2008 Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. The Journal of biological chemistry 17 18667423
2024 Enhancing m6A modification of lncRNA through METTL3 and RBM15 to promote malignant progression in bladder cancer. Heliyon 16 38560117
2024 Epigenetic mechanism of RBM15 in affecting cisplatin resistance in laryngeal carcinoma cells by regulating ferroptosis. Biology direct 16 39039611
2024 RBM15 promotes lipogenesis and malignancy in gastric cancer by regulating N6-Methyladenosine modification of ACLY mRNA in an IGF2BP2-dependent manner. Biochimica et biophysica acta. Molecular and cell biology of lipids 16 39549859
2011 Acute megakaryoblastic leukemia with a four-way variant translocation originating the RBM15-MKL1 fusion gene. Pediatric blood & cancer 16 21370421
2025 RBM15 recruits myeloid-derived suppressor cells via the m6A-IGF2BP3/CBR3-AS1/miR-409-3p/CXCL1 axis, facilitating radioresistance in non-small-cell lung cancer. Journal of translational medicine 15 39962467
2024 The m6A methyltransferase RBM15 affects tumor cell stemness and progression of cervical cancer by regulating the stability of lncRNA HEIH. Experimental cell research 15 38280435
2024 RBM15 Protects From Myocardial Infarction by Stabilizing NAE1. JACC. Basic to translational science 15 38984049
2022 RBM15 condensates modulate m6A modification of STYK1 to promote tumorigenesis. Computational and structural biotechnology journal 15 36147665
2025 Lactylation increases the stability of RBM15 to drives m6A modification in non-small-cell lung cancer cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 14 40135634
2024 Exploring the role of m 6 A writer RBM15 in cancer: a systematic review. Frontiers in oncology 14 38915367
2020 Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish. The Journal of biological chemistry 14 32518161
2005 RBM15-MKL1 (OTT-MAL) fusion transcript in an adult acute myeloid leukemia patient. American journal of hematology 14 15849773
1989 An immunohistochemical characterization of the primitive and maturing neuroepithelial components in the OTT-6050 transplantable mouse teratoma. Neuropathology and applied neurobiology 14 2586719
2024 RBM15 drives the progression of lung adenocarcinoma by regulating N6-methyladenosine-mediated LDHA mRNA stability. Life sciences 13 39406308
2024 MLL1 promotes placental trophoblast ferroptosis and aggravates preeclampsia symptoms through epigenetic regulation of RBM15/TRIM72/ADAM9 axis. Biology direct 13 39709463
2010 Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57. Virology 12 20828777
2024 RBM15 Knockdown Impairs the Malignancy of Cervical Cancer by Mediating m6A Modification of Decorin. Biochemical genetics 11 38429603
2024 Identification of m6A modification patterns and RBM15 mediated macrophage phagocytosis in pancreatic cancer: An integrative analysis. Biochimica et biophysica acta. Molecular basis of disease 11 38878830
2024 RBM15 Drives Breast Cancer Cell Progression and Immune Escape via m6A-Dependent Stabilization of KPNA2 mRNA. Clinical breast cancer 11 39488447
2024 RBM15-Mediated N6-Methyl Adenosine (m6A) Modification of EZH2 Drives the Epithelial-Mesenchymal Transition of Cervical Cancer. Critical reviews in eukaryotic gene expression 10 38842201
2024 RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells. Molecular medicine (Cambridge, Mass.) 10 39716068
2016 New hPSC-based human models to study pediatric Acute Megakaryoblastic Leukemia harboring the fusion oncogene RBM15-MKL1. Stem cell research 10 28412998
2023 Resveratrol affects ccRCC cell senescence and macrophage polarization by regulating the stability of CCNB1 by RBM15. Epigenomics 9 37909116
2025 RBM15-mediated m6A modification of XPR1 promotes the malignant progression of lung adenocarcinoma. Naunyn-Schmiedeberg's archives of pharmacology 8 39928150
2025 RBM15-MKL1 fusion protein promotes leukemia via m6A methylation and Wnt pathway activation. Blood 7 40435410
2024 Overexpression of RBM15 modulated the effect of trophoblast cells by promoting the binding ability between YTHDF2 and the CD82 3'UTR to decrease the expression of CD82. Heliyon 7 38765115
2024 RNA methylase RBM15 facilitates malignant progression of colorectal cancer through regulating E2F2 in an m6A modification-dependent manner. Journal of biochemical and molecular toxicology 7 39403943
1981 Neural differentiation in the OTT-6050 mouse teratoma. Production of a tumor fraction restricted to stem cells and neural cells after centrifugal elutriation. Virchows Archiv. A, Pathological anatomy and histology 7 7269228
2025 RBM15, an m6A enzyme, suppresses NLRP3 inflammasome activation in rheumatoid arthritis through macrophage metabolism. Clinical and experimental rheumatology 6 40095631
2025 RBM15 enhances paclitaxel resistance in triple-negative breast cancer by targeting m6A methylation of TNFSF9 and inducing polarization of tumor-associated macrophages to M2 phenotype. Hereditas 6 40830812
2025 Hypoxia-Induced Histone Lactylation Drives Cisplatin Resistance in Bladder Cancer by Promoting RBM15-Dependent m6A Methylation of IGFBP3. Research (Washington, D.C.) 6 41199784
2024 The RNA m6A writer RBM15 contributes to the progression of esophageal squamous cell carcinoma by regulating miR-3605-5p/KRT4 pathway. Heliyon 6 38312624
2024 RBM15 activates glycolysis in M1-type macrophages to promote the progression of aortic aneurysm and dissection. International journal of medical sciences 6 39113895
2023 RBM15-Mediated m6A Modification of K17 Affects Keratinocytes Response to IL-17A Stimulation in Psoriasis. Annals of clinical and laboratory science 6 37625827
2025 Role of N6-methyladenosine methyltransferase component RBM15 in cancer progression and its therapeutic potential. Discover oncology 5 40402374
2023 THE ROLE OF N6-METHYLADENOSINE METHYLTRANSFERASE RBM15 IN NONALCOHOLIC FATTY LIVER DISEASE. Shock (Augusta, Ga.) 5 38150369
2011 Ott's protein osmotic pressure of serum and interstitial fluid in chickens (Gallus gallus): effect of age and gender. The Journal of experimental biology 5 21270308
2025 RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4. Hereditas 4 40682199
2024 RBM15-mediated the m6A modification of MAT2A promotes osteosarcoma cell proliferation, metastasis and suppresses ferroptosis. Molecular and cellular biochemistry 4 39527319
2025 RBM15 promotes m6A methylation and stability of KLF6 mRNA to accelerate pyroptosis of retinal ganglion cells in early-stage diabetic retinopathy. Journal of molecular histology 3 40464812
2025 RNA Binding Motif Protein 15 (RBM15): Structure, Function and Its Research Progress in Tumors. International journal of general medicine 3 40636291
2024 Myeloid sarcoma with RBM15::MRTFA (MKL1) mimicking vascular neoplasm. Virchows Archiv : an international journal of pathology 3 38374236
2024 RBM15 Promotes High Glucose-Induced Lens Epithelial Cell Injury by Inducing PRNP N6-Methyladenine Modification During Diabetic Cataract. Current eye research 3 39206850
2024 RBM15 increase tumor-infiltrating CD4+ T cell in ESCC via modulating of PLOD3. American journal of cancer research 3 39659928
2025 m6A Methylation Regulator RBM15-Mediated Upregulation of ITGBL1 mRNA Stability Aggravates Colon Adenocarcinoma Progression by Remodeling the Tumor Microenvironment. The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 2 39840822
2025 RBM15 relies on m6A modification to inhibit UBE2C, alleviating hippocampal neuronal injury by limiting microglial inflammation. Molecular and cellular neurosciences 2 40049430
2025 RBM15-mediated metabolic reprogramming boosts immune response in colorectal cancer. Frontiers in immunology 2 40370450
2025 lncSLERT Promotes Liver Metastasis in Colorectal Cancer by Down-Regulating HUNK Expression via RBM15-Mediated m6A Modification. OncoTargets and therapy 2 40371234
2025 RBM15 promotes COAD progression by regulating the m6A modification of TMC5. Hereditas 2 40883807
2025 RBM15 Mediated m6A Modification of SRSF1 Inhibits Cuproptosis in Non-Small Cell Lung Cancer by Mediating ATP7B Alternative Splicing. The Kaohsiung journal of medical sciences 2 40923717
2024 N6-Methyladenosine Modification of PERP by RBM15 Enhances the Tumorigenesis of Lung Adenocarcinoma via p53 Signaling Pathway. Molecular biotechnology 2 39556280
2024 Mechanism of RBM15 in the malignant proliferation of colorectal cancer cells through regulating the stability of LncRNA FGD5-AS1 via m6A modification. Experimental cell research 2 39701357
2025 RBM15-MKL1 fusion protein promotes leukemia via m6A methylation and WNT pathway activation. bioRxiv : the preprint server for biology 1 40060447
2025 RBM15-mediated m6A methylation of Entpd1/CD39 regulates extracellular ATP hydrolysis and alleviates myocardial ischemia-reperfusion injury. International immunopharmacology 1 40614597
2025 RBM15-mediated m6A modification in cancer progression and tumor immunity: molecular mechanisms and therapeutic potential. Frontiers in cell and developmental biology 1 41403702
2025 RBM15 in diseases: Molecular mechanisms and clinical opportunities from RNA m6A methylation. Genes & diseases 1 41674656

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