Affinage

RBM15

RNA-binding protein 15 · UniProt Q96T37

Length
977 aa
Mass
107.2 kDa
Annotated
2026-04-28
100 papers in source corpus 24 papers cited in narrative 24 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RBM15 is a multifunctional RNA-binding protein that serves as the mRNA-targeting adaptor of the m6A methyltransferase complex and regulates mRNA export, alternative splicing, and epigenetic gene control in hematopoiesis and development. Through its three RNA recognition motifs, RBM15 binds pre-mRNA intronic regions and specific transcripts (e.g., XIST, VEGFA, GATA1, c-MPL) to recruit the METTL3/WTAP methyltransferase via the bridging factor ZC3H13, directing m6A deposition that controls mRNA stability and fate (PMID:29535189, PMID:26575292, PMID:32258426); its C-terminal SPOC domain mediates interactions with the histone methyltransferase SETD1B and HDAC3, coupling RNA processing to chromatin state at target loci (PMID:22927943, PMID:25468569). RBM15 also promotes bulk mRNA nuclear export by facilitating DBP5 access to NXF1-mRNP complexes at the nuclear pore (PMID:19786495). RBM15 protein stability is controlled by PRMT1-mediated arginine methylation at R578, which triggers CNOT4-dependent ubiquitination and degradation, and by lactylation at K850, which stabilizes RBM15 and is required for its association with METTL3 (PMID:26575292, PMID:40135634).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2001 High

    Identification of RBM15 as an RRM-containing protein fused to MKL1 in t(1;22) acute megakaryoblastic leukemia established it as a candidate RNA-binding regulator in leukemogenesis, motivating all subsequent functional studies.

    Evidence Molecular cloning of translocation breakpoint and domain analysis in AMKL patient samples

    PMID:11431691

    Open questions at the time
    • No functional activity demonstrated at this stage
    • Endogenous RNA targets unknown
    • Normal cellular role undefined
  2. 2007 High

    Demonstration that RBM15 modulates Notch/RBPJ-dependent transcription and that conditional Rbm15 deletion disrupts B-cell development and expands myeloid/megakaryocytic compartments established RBM15 as a hematopoietic lineage regulator operating through Notch signaling.

    Evidence Co-immunoprecipitation with RBPJκ, promoter-reporter assays in hematopoietic vs. non-hematopoietic cells; conditional knockout mouse with flow cytometry and transplantation

    PMID:17283045 PMID:17376872

    Open questions at the time
    • Mechanism by which an RNA-binding protein modulates transcription factor activity unclear
    • Direct vs. indirect effect on Notch pathway not resolved
    • Whether splicing regulation underlies lineage defects untested
  3. 2008 High

    Germline knockout lethality traced to trophoblast-autonomous placental failure, and identification of HDAC3 as an RBM15 interactor, revealed essential developmental roles and linked RBM15 to chromatin-modifying complexes.

    Evidence Germline and trophoblast-specific rescue KO mice with placental histology; Co-IP of RBM15 with HDAC3 and promoter-reporter assays

    PMID:18667423 PMID:18981216

    Open questions at the time
    • Direct chromatin targets of RBM15-HDAC3 complex undefined
    • Whether RBM15 is recruited to chromatin via RNA binding not tested
  4. 2009 High

    Discovery that RBM15 binds DBP5, colocalizes with NXF1 at the nuclear envelope, and is required for bulk mRNA export established a second major function—mRNA nuclear export—independent of its transcriptional roles.

    Evidence Co-IP, RNAi knockdown with nuclear/cytoplasmic mRNA fractionation, immunofluorescence colocalization in HeLa cells

    PMID:19786495

    Open questions at the time
    • Whether export and splicing functions are coupled unknown
    • Specific mRNA cargo selectivity not determined
    • Structural basis of DBP5 interaction unresolved
  5. 2010 High

    Demonstration that RBM15 binds a viral RNA (KSHV ORF59) via its SPOC domain and that KSHV ORF57 hijacks this interaction established the SPOC domain as a critical protein-interaction surface mediating RNA regulatory functions.

    Evidence Direct protein interaction mapping, RNA immunoprecipitation, nucleocytoplasmic fractionation of viral RNA upon RBM15 manipulation

    PMID:21106733

    Open questions at the time
    • Generality of SPOC domain as a protein-interaction hub for cellular partners not yet shown
    • Whether viral exploitation reflects a normal cellular pathway unclear
  6. 2012 High

    Identification of SETD1B as a SPOC-domain-dependent RBM15 partner required for leukemogenic proliferation, and demonstration that RBM15 preserves HSC quiescence under replicative stress, broadened understanding to include epigenetic regulation and stem cell maintenance.

    Evidence Co-IP with domain mutagenesis and cytokine-independence proliferation assay; conditional KO with competitive transplantation, cell cycle, and ROS analysis

    PMID:22490678 PMID:22927943

    Open questions at the time
    • Which histone modifications are RBM15-dependent genome-wide not mapped
    • Whether stress-dependent HSC failure involves m6A or splicing changes untested
  7. 2014 High

    Showing that RBM15 binds c-Mpl RNA, occupies its chromatin locus, controls H4ac/H3K4me3 marks, and determines alternative splicing of a dominant-negative c-Mpl isoform unified the RNA-binding and chromatin-modifying activities into a single mechanistic model for splicing regulation.

    Evidence RNA immunoprecipitation, ChIP, alternative splicing assays, HDAC/HMT inhibitor treatment in conditional KO HSCs

    PMID:25468569

    Open questions at the time
    • Whether co-transcriptional mechanism applies to other RBM15 splicing targets untested
    • Structural basis of simultaneous RNA and chromatin binding unknown
  8. 2015 High

    Reconstitution of the PRMT1→R578 methylation→CNOT4 ubiquitination→degradation cascade, and demonstration that RBM15 recruits SF3B1 to pre-mRNA intronic regions of key hematopoietic genes, defined the post-translational control of RBM15 turnover and its direct role in spliceosome recruitment.

    Evidence In vitro methylation/ubiquitylation assays, mass spectrometry, RIP, RNA-seq splicing analysis, rescue experiments in AMKL cells

    PMID:26575292

    Open questions at the time
    • Whether other E3 ligases contribute to RBM15 degradation not excluded
    • How SF3B1 recruitment specificity is achieved unknown
  9. 2015 High

    Identification of RBM15 as required for Xist-mediated X chromosome silencing, and colocalization with Xist RNA at the nuclear matrix, linked RBM15's RNA-binding function to dosage compensation and non-coding RNA biology.

    Evidence Pooled shRNA screen in mouse ESCs, super-resolution 3D-SIM microscopy

    PMID:26190105

    Open questions at the time
    • Whether RBM15 acts via m6A deposition on Xist or via an independent mechanism not resolved at this point
  10. 2018 High

    Demonstration that ZC3H13 bridges RBM15 (Nito) to WTAP (Fl(2)d) to recruit METTL3 to RNA substrates established the core topology of the m6A writer complex and positioned RBM15 as the mRNA-targeting adaptor.

    Evidence Reciprocal Co-IP in Drosophila and mammalian cells, m6A quantification, genetic epistasis using sex determination phenotype

    PMID:29535189

    Open questions at the time
    • RNA sequence/structural determinants of RBM15 target selectivity not defined
    • Stoichiometry of the complex unresolved
  11. 2020 High

    CRISPR deletion of the Xist A-repeat abolishing RBM15-dependent m6A deposition at the Xist 5' region, and proteomic identification of RBM15's interactome including SETD1B and m6A complex components, mechanistically connected RBM15's m6A activity to X-inactivation.

    Evidence CRISPR/Cas9 deletion, m6A-seq, allelic RNA-seq, MS/MS interactome from epitope-tagged RBM15 pulldown in mouse ESCs

    PMID:32258426

    Open questions at the time
    • Whether loss of 5' m6A alone is sufficient for silencing failure not fully resolved
    • Redundancy with RBM15B not quantified
  12. 2022 Medium

    Observation that RBM15 forms RNA-enhanced nuclear liquid-like condensates that partially colocalize with m6A-modified transcripts introduced a biophysical dimension to understanding how RBM15 concentrates its m6A-writing activity on select targets.

    Evidence Super-resolution microscopy, in vitro phase separation assay, MeRIP-seq, oncogenic transformation assay (STYK1/MAPK axis)

    PMID:36147665

    Open questions at the time
    • Functional necessity of phase separation for m6A activity not demonstrated by condensate-disrupting mutations
    • Whether condensates form in primary cells unknown
    • In vivo relevance of STYK1 regulation not confirmed
  13. 2025 Medium

    Discovery that K850 lactylation stabilizes RBM15 and is required for METTL3 association and global m6A deposition, with HDAC3 acting as the delactylase, added a metabolite-sensing layer to RBM15 regulation and connected metabolic state to epitranscriptomic output.

    Evidence Lactylation site mapping, K850R mutagenesis, HDAC3 delactylase assay, Co-IP and m6A quantification

    PMID:40135634

    Open questions at the time
    • Physiological conditions that regulate K850 lactylation in vivo not defined
    • Interplay between R578 methylation and K850 lactylation unexplored
    • Structural mechanism by which lactylation promotes METTL3 binding unknown
  14. 2025 High

    Multi-omic dissection of the RBM15-MKL1 fusion showed it retains m6A-writing function but selectively targets Wnt/Frizzled pathway mRNAs; METTL3 inhibition and Frizzled knockdown suppressed AMKL growth in vivo, validating m6A-dependent Wnt signaling as a therapeutic vulnerability.

    Evidence Transcriptome-wide RNA binding, m6A methylation, RNA turnover profiling; METTL3 inhibitor STM3675 in vivo; Frizzled genetic knockdown in transplantation model

    PMID:40435410

    Open questions at the time
    • Whether wild-type RBM15 also targets Frizzled mRNAs in normal hematopoiesis unknown
    • Mechanism of target selectivity shift in the fusion protein not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • The rules governing RBM15's RNA target selectivity, the structural basis for simultaneous engagement with chromatin and RNA, and the functional interplay among its multiple post-translational modifications (methylation, ubiquitination, lactylation) in physiological contexts remain to be determined.
  • No genome-wide map of RBM15-dependent m6A sites in primary hematopoietic cells
  • No high-resolution structure of full-length RBM15 or its complex with METTL3/WTAP/ZC3H13
  • Functional redundancy between RBM15 and RBM15B not systematically defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 8 GO:0140110 transcription regulator activity 3 GO:0060090 molecular adaptor activity 2
Localization
GO:0005634 nucleus 4 GO:0005654 nucleoplasm 2 GO:0005635 nuclear envelope 1
Pathway
R-HSA-8953854 Metabolism of RNA 7 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-1266738 Developmental Biology 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-4839726 Chromatin organization 3
Partners
CNOT4DBP5HDAC3METTL3SETD1BSF3B1WTAPZC3H13
Complex memberships
SETD1B histone methyltransferase complexm6A methyltransferase complex (METTL3/WTAP/ZC3H13/RBM15)

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 RBM15 (OTT1) was identified as a novel RNA recognition motif-containing protein with homology to Drosophila spen, fused to MKL1 in the t(1;22)(p13;q13) translocation of acute megakaryoblastic leukemia, establishing RBM15 as an RNA-binding protein involved in leukemogenesis. Molecular cloning and characterization of translocation breakpoint; domain analysis Nature genetics High 11431691
2007 RBM15 modulates Notch-induced HES1 promoter activity in a cell-type-specific manner: it inhibits Notch-induced HES1 transcription in non-hematopoietic cells but stimulates it in hematopoietic cell lines. The N-terminus of RBM15 co-immunoprecipitates with RBPJkappa, a critical Notch signaling transcription factor, and the N-terminus acts as a dominant negative to impair full-length RBM15 activation of HES1. Co-immunoprecipitation, promoter-luciferase reporter assays, RNA interference knockdown, enforced expression Molecular and cellular biology High 17283045
2007 Conditional deletion of Ott1 (Rbm15) in adult mice causes loss of peripheral B cells due to a pro/pre-B differentiation block, myeloid and megakaryocytic expansion, increased Lin-Sca-1+c-Kit+ compartment, and a shift in progenitor fate toward granulocyte differentiation, establishing Ott1 as required for B lymphopoiesis and as an inhibitory regulator in myeloid, megakaryocytic, and progenitor compartments. Conditional knockout mouse model (Cre-lox), flow cytometry, bone marrow transplantation Proceedings of the National Academy of Sciences of the United States of America High 17376872
2008 Germ-line Ott1 (Rbm15) deletion causes fetal demise by E10.5 due to placental defects in spongiotrophoblast and syncytiotrophoblast layers arresting vascular branching morphogenesis; trophoblast-specific rescue showed this is regulated by the trophoblast compartment rather than fetal vasculature. Surviving conditional mice showed hyposplenia and abnormal cardiac development with enriched hypoxia-related gene expression. Germline and conditional knockout mouse models, placental histology, global gene expression analysis, trophoblast-sparing Cre rescue Molecular and cellular biology High 18981216
2008 OTT (RBM15) interacts with histone deacetylase 3 (HDAC3), but this interaction is abolished in the OTT-BSAC fusion protein. OTT-BSAC, unlike OTT alone, localizes exclusively to the nucleus (OTT normally shows cytoplasmic BSAC partner activity) and strongly activates promoters containing Yin Yang 1-binding sequences. Co-immunoprecipitation, subcellular localization (immunofluorescence), promoter-luciferase reporter assays The Journal of biological chemistry Medium 18667423
2009 RBM15 binds specifically to the mRNA export helicase DBP5 and facilitates its direct contact with mRNA in vivo. RBM15 localizes to the nuclear envelope where it colocalizes with DBP5 and NXF1. Gene silencing of RBM15 causes cytoplasmic depletion and nuclear accumulation of bulk mRNA and individual transcripts, demonstrating RBM15 is required for efficient mRNA export. Co-immunoprecipitation, RNAi knockdown, subcellular fractionation, immunofluorescence colocalization, RNA export assays Nucleic acids research High 19786495
2009 The OTT-MAL (RBM15-MKL1) fusion oncogene deregulates transcriptional activity of the Notch signaling transcription factor RBPJ and causes abnormal fetal megakaryopoiesis; cooperation with an activating MPL mutation efficiently induces AMKL in a knockin mouse model, establishing concomitant activation of RBPJ and MPL as leukemogenic in the megakaryocytic lineage. Knockin mouse model, transcriptional reporter assays, genetic epistasis (OTT-MAL + MPLW515L cooperation) The Journal of clinical investigation High 19287095
2010 KSHV ORF57 interacts directly with the C-terminal SPOC domain of RBM15 to reduce RBM15 binding to ORF59 RNA and shift ORF59 RNA accumulation from nuclear to cytoplasmic. RBM15 (and OTT3) are cellular NXF1 cofactors that participate in ORF57-enhanced viral gene expression; RBM15 promotes nuclear accumulation and hyperpolyadenylation of ORF59 RNA in the absence of ORF57. Direct protein interaction assays, RNA immunoprecipitation, nucleocytoplasmic fractionation, RNAi knockdown, domain mapping Journal of virology High 21106733
2012 Both RBM15 and the leukemogenic RBM15-MKL1 fusion protein interact directly with the Setd1b histone H3-Lys4 methyltransferase via the RBM15 SPOC domain and the Setd1b LSD motif. This interaction is required for cytokine-independent proliferation driven by RBM15-MKL1 overexpression, implicating altered Setd1b-dependent epigenetic regulation in AMKL leukemogenesis. Co-immunoprecipitation, domain mutagenesis (SPOC domain), cell proliferation/cytokine-independence assays PloS one High 22927943
2012 Ott1 (Rbm15) is required to preserve hematopoietic stem cell quiescence specifically under replicative stress (not steady-state hematopoiesis); Ott1-deleted HSCs show reduced G0 fraction and fail to repopulate after transplantation. Loss of Ott1 increases NF-κB activation, DNA damage, and reactive oxygen species in HSCs, and Ott1-deleted HSCs share a gene expression signature with aged HSCs. Conditional knockout mouse, competitive transplantation, cell cycle analysis (flow cytometry), ROS measurement, gene expression profiling Blood High 22490678
2014 Ott1 (Rbm15) controls alternative splicing of c-Mpl, producing a dominant-negative isoform (Mpl-TR) that inhibits HSC engraftment and attenuates thrombopoietin signaling. Ott1 binds both c-Mpl RNA and chromatin and regulates H4 acetylation and H3K4me3 marks at the c-Mpl locus; HDAC or histone methyltransferase inhibition increases Mpl-TR levels, indicating Ott1 uses an epigenetic mechanism to control alternative splicing. Ott1 associates with Hdac3 and the histone methyltransferase Setd1b. RNA immunoprecipitation, chromatin immunoprecipitation, alternative splicing assays, HDAC/HMT inhibitor treatment, conditional knockout HSC analysis Blood High 25468569
2015 RBM15 is methylated by PRMT1 at residue R578, which leads to its ubiquitylation and degradation by the E3 ligase CNOT4. PRMT1 overexpression in acute megakaryocytic leukemia cell lines blocks megakaryocyte terminal differentiation by reducing RBM15 protein level; restoring RBM15 rescues differentiation. At the molecular level, RBM15 binds pre-mRNA intronic regions of GATA1, RUNX1, TAL1, and c-MPL genes and recruits the splicing factor SF3B1 to the same sites for alternative splicing. In vitro methylation assay, ubiquitylation assay, mass spectrometry, RNA immunoprecipitation, RNA-seq/splicing analysis, domain mutagenesis, rescue experiments eLife High 26575292
2015 An shRNA screen identified RBM15, Spen, and Wtap as factors required for Xist RNA-mediated X chromosome silencing. RBM15 co-localizes with Xist RNA within the nuclear matrix subcompartment as demonstrated by super-resolution 3D-SIM microscopy, consistent with direct involvement in Xist-mediated gene silencing. Pooled shRNA screen, validation knockdown experiments, super-resolution 3D-SIM microscopy colocalization Cell reports High 26190105
2016 An antisense RNA (AS-RBM15) transcribed from within exon 1 of RBM15 enhances RBM15 protein translation in a CAP-dependent manner through the region overlapping the 5'UTR of RBM15. AS-RBM15 expression is activated by RUNX1 and repressed by RUNX1-ETO, and its upregulation enhances megakaryocyte terminal differentiation. Antisense RNA overexpression/knockdown, polysome profiling, CAP-dependent translation assays, RUNX1 ChIP, reporter assays EMBO reports High 27118388
2018 Zc3h13 (Flacc in Drosophila) promotes m6A deposition by bridging Fl(2)d (WTAP ortholog) to the mRNA-binding factor Nito (RBM15 ortholog), placing RBM15/Nito as the mRNA-binding adapter that recruits the m6A methyltransferase complex to substrate mRNAs. Co-immunoprecipitation in Drosophila and mice, m6A methylation quantification, genetic epistasis (sex determination phenotype), mass spectrometry interactome Genes & development High 29535189
2019 RBM15, as a subunit of the m6A methyltransferase complex, interacts with BAF155 mRNA and mediates its degradation through the mRNA methylation machinery. Ablation of RBM15 augments BAF155 expression; RBM15 overexpression decreases BAF155 mRNA and protein levels. The regulation of BAF155 by RBM15 depends on the activity of the core catalytic subunit METTL3. This disrupts BAF155-dependent transcriptional activity and delamination of apical radial glial progenitors in developing cortex. RNA immunoprecipitation, overexpression/knockdown in neuronal cells and in vivo cortex, m6A inhibition via METTL3 KO, transcriptional activity assays Molecular neurobiology Medium 31020615
2020 RBM15 binds to the Xist A-repeat, and deletion of the A-repeat entirely abolishes deposition of m6A in the Xist 5' m6A region without affecting exon VII m6A. Purified epitope-tagged RBM15 from mouse ESCs interacts with the m6A complex, the SETD1B histone modifying complex, and several RNA metabolism proteins. The Xist 5' m6A region plays a role in Xist-mediated chromosome silencing. CRISPR/Cas9 deletion, m6A-seq, allelic RNA-seq, MS/MS interactome (epitope-tagged RBM15 pulldown), Xist RNA FISH Wellcome open research High 32258426
2020 Loss of rbm15 in zebrafish specifically impairs liver maturation without affecting hepatoblast specification, differentiation, or hepatocyte proliferation/apoptosis. The mTORC1 pathway is hyperactivated in rbm15-deficient hepatocytes, and rapamycin treatment partially restores normal hepatic gene expression and nuclear localization of the transcription factor Hnf4a. Zebrafish ENU screen, positional cloning, CRISPR/Cas9 knockout, rapamycin treatment, gene expression analysis The Journal of biological chemistry Medium 32518161
2022 RBM15 forms liquid-like condensates (phase separation) dispersed in the nucleus, undergoing dynamic fusion and fission activities that partially colocalize with m6A-modified transcripts. Addition of RNA enhances RBM15 phase-separation propensity in vitro. RBM15 preferentially binds to and promotes m6A modification of STYK1 mRNA, enhancing its stability, and upregulated STYK1 causes MAPK hyperactivation driving oncogenic transformation. Super-resolution structured illumination microscopy, in vitro phase separation assay, MeRIP-seq, RNA-seq, oncogenic transformation assay Computational and structural biotechnology journal Medium 36147665
2023 AZGP1P2 binds to UBA1 and RBM15 as a compound; UBA1, an E1 ubiquitin-activating enzyme, contributes to RBM15 protein degradation via ubiquitination modification, providing an additional ubiquitin-dependent regulatory mechanism for RBM15 protein stability. RNA pull-down with mass spectrometry, co-immunoprecipitation, RNA immunoprecipitation, MeRIP assay Research (Washington, D.C.) Medium 37854295
2025 Lactylation of RBM15 at Lys850 (K850), mediated by lactate uptake via MCT1, stabilizes RBM15 by inhibiting proteasome-mediated ubiquitin degradation. HDAC3 acts as the delactylase for RBM15. The K850R lactylation-site mutation disrupts the association between RBM15 and METTL3, reducing global m6A levels and abrogating RBM15-mediated cell proliferation and migration. Lactylation site mapping, HDAC3 delactylase assay, K850R mutagenesis, Co-IP (RBM15-METTL3 interaction), m6A quantification, cell proliferation assays FASEB journal Medium 40135634
2025 The RBM15-MKL1 fusion protein retains the RNA-binding and m6A-modifying functions of RBM15 while selectively regulating distinct mRNA targets including Frizzled genes in the Wnt signaling pathway. METTL3 inhibition (STM3675) induced apoptosis in RBM15-MKL1 AMKL cells and prolonged survival in transplanted mice. Direct Frizzled knockdown reduced AMKL growth in vitro and in vivo, establishing Wnt signaling as a key m6A-dependent oncogenic driver. Multi-omics (transcriptome-wide RNA binding, m6A methylation, RNA turnover), METTL3 inhibitor treatment, genetic knockdown (Frizzled genes), mouse transplantation model Blood High 40435410
2024 RBM15-mediated m6A modification of VEGFA mRNA is recognized and stabilized by IGF2BP3 and YTHDF2, leading to enhanced VEGFA expression and VEGFA-related angiogenic functions (HUVEC migration and tube formation). Knockdown of RBM15 in HCC xenografts reduces VEGFA expression and inhibits tumor growth linked to angiogenesis. MeRIP-seq, RNA-seq, CLIP-seq integration, cell biology (HUVEC migration/tube formation), xenograft model, RIP assay Molecular carcinogenesis Medium 39092767
2024 Hypoxia-driven H3K18 lactylation (catalyzed by KAT2B) activates RBM15 transcription; RBM15 then stabilizes IGFBP3 mRNA via m6A modification dependent on its SPOC domain, with nuclear IGFBP3 complexing with p-EGFR/p-DNA-PKcs to enhance DNA repair and cisplatin resistance in bladder cancer. ChIP (H3K18la on RBM15 promoter), MeRIP (m6A of IGFBP3), SPOC domain requirement shown, Co-IP (IGFBP3/p-EGFR/p-DNA-PKcs complex), in vivo xenograft with LDHA + EGFR inhibitor combination Research (Washington, D.C.) Medium 41199784

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes & development 507 29535189
2001 Fusion of two novel genes, RBM15 and MKL1, in the t(1;22)(p13;q13) of acute megakaryoblastic leukemia. Nature genetics 239 11431691
2015 A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing. Cell reports 227 26190105
2021 RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. Journal of experimental & clinical cancer research : CR 203 33637103
2015 Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. eLife 157 26575292
2009 The OTT-MAL fusion oncogene activates RBPJ-mediated transcription and induces acute megakaryoblastic leukemia in a knockin mouse model. The Journal of clinical investigation 94 19287095
2007 Rbm15 modulates Notch-induced transcriptional activation and affects myeloid differentiation. Molecular and cellular biology 87 17283045
2005 Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. The Journal of biological chemistry 81 16129689
2007 Ott1(Rbm15) has pleiotropic roles in hematopoietic development. Proceedings of the National Academy of Sciences of the United States of America 79 17376872
2016 The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation. EMBO reports 76 27118388
2022 Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell proliferation 72 36181462
2021 RBM15 promotes hepatocellular carcinoma progression by regulating N6-methyladenosine modification of YES1 mRNA in an IGF2BP1-dependent manner. Cell death discovery 70 34707107
2009 Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Nucleic acids research 55 19786495
2022 Epigenetic activation of RBM15 promotes clear cell renal cell carcinoma growth, metastasis and macrophage infiltration by regulating the m6A modification of CXCL11. Free radical biology & medicine 52 35381326
2021 Knockdown RBM15 Inhibits Colorectal Cancer Cell Proliferation and Metastasis Via N6-Methyladenosine (m6A) Modification of MyD88 mRNA. Cancer biotherapy & radiopharmaceuticals 49 34842457
2019 RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex. Molecular neurobiology 48 31020615
2008 Ott1 (Rbm15) is essential for placental vascular branching morphogenesis and embryonic development of the heart and spleen. Molecular and cellular biology 46 18981216
2010 Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59. Journal of virology 45 21106733
2020 The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation. Wellcome open research 43 32258426
2014 Ott1 (Rbm15) regulates thrombopoietin response in hematopoietic stem cells through alternative splicing of c-Mpl. Blood 42 25468569
2012 Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation. PloS one 42 22927943
2002 Recurrence of OTT-MAL fusion in t(1;22) of infant AML-M7. Genes, chromosomes & cancer 42 11746984
2023 AZGP1P2/UBA1/RBM15 Cascade Mediates the Fate Determinations of Prostate Cancer Stem Cells and Promotes Therapeutic Effect of Docetaxel in Castration-Resistant Prostate Cancer via TPM1 m6A Modification. Research (Washington, D.C.) 41 37854295
2024 The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism. Experimental & molecular medicine 35 38825643
2021 RBM15-mediated N6-methyladenosine modification affects COVID-19 severity by regulating the expression of multitarget genes. Cell death & disease 34 34301919
2018 MED12, TERT promoter and RBM15 mutations in primary and recurrent phyllodes tumours. British journal of cancer 33 29315289
2012 Hematopoietic stem cells lacking Ott1 display aspects associated with aging and are unable to maintain quiescence during proliferative stress. Blood 33 22490678
2023 RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4. Molecular medicine (Cambridge, Mass.) 32 36803098
2023 RBM15 silencing promotes ferroptosis by regulating the TGF-β/Smad2 pathway in lung cancer. Environmental toxicology 30 36715115
2023 Knockdown of RBM15 inhibits tumor progression and the JAK-STAT signaling pathway in cervical cancer. BMC cancer 30 37474926
2016 RBM15 Functions in Blood Diseases. Current cancer drug targets 30 26758534
2023 RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines. Cancers 29 36831430
2023 RBM15 m6 A modification-mediated OTUB2 upregulation promotes cervical cancer progression via the AKT/mTOR signaling. Environmental toxicology 27 37334762
2012 Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells. Leukemia & lymphoma 27 22497198
2023 RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer. International journal of oncology 26 37594126
2023 N6-methyladenosine methylation regulator RBM15 promotes the progression of diabetic nephropathy by regulating cell proliferation, inflammation, oxidative stress, and pyroptosis through activating the AGE-RAGE pathway. Environmental toxicology 23 37551785
1996 Ott, a mouse X-linked multigene family expressed specifically during meiosis. Human molecular genetics 23 8842733
2024 m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma. Molecular carcinogenesis 22 39092767
2024 RBM15 facilitates osimertinib resistance of lung adenocarcinoma through m6A-dependent epigenetic silencing of SPOCK1. Oncogene 22 39528815
2015 Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15. Viruses 21 25690794
2023 HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition. Cell biology international 20 37191290
2018 PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Experimental and therapeutic medicine 19 29456659
2008 Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. The Journal of biological chemistry 17 18667423
2024 RBM15 facilities lung adenocarcinoma cell progression by regulating RASSF8 stability through N6 Methyladenosine modification. Translational oncology 16 38838436
2024 Enhancing m6A modification of lncRNA through METTL3 and RBM15 to promote malignant progression in bladder cancer. Heliyon 15 38560117
2024 RBM15 promotes lipogenesis and malignancy in gastric cancer by regulating N6-Methyladenosine modification of ACLY mRNA in an IGF2BP2-dependent manner. Biochimica et biophysica acta. Molecular and cell biology of lipids 15 39549859
2024 Exploring the role of m 6 A writer RBM15 in cancer: a systematic review. Frontiers in oncology 14 38915367
2024 Epigenetic mechanism of RBM15 in affecting cisplatin resistance in laryngeal carcinoma cells by regulating ferroptosis. Biology direct 14 39039611
2022 RBM15 condensates modulate m6A modification of STYK1 to promote tumorigenesis. Computational and structural biotechnology journal 14 36147665
2020 Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish. The Journal of biological chemistry 14 32518161
2011 Acute megakaryoblastic leukemia with a four-way variant translocation originating the RBM15-MKL1 fusion gene. Pediatric blood & cancer 14 21370421
2005 RBM15-MKL1 (OTT-MAL) fusion transcript in an adult acute myeloid leukemia patient. American journal of hematology 14 15849773
1989 An immunohistochemical characterization of the primitive and maturing neuroepithelial components in the OTT-6050 transplantable mouse teratoma. Neuropathology and applied neurobiology 14 2586719
2024 The m6A methyltransferase RBM15 affects tumor cell stemness and progression of cervical cancer by regulating the stability of lncRNA HEIH. Experimental cell research 13 38280435
2024 RBM15 Protects From Myocardial Infarction by Stabilizing NAE1. JACC. Basic to translational science 13 38984049
2025 RBM15 recruits myeloid-derived suppressor cells via the m6A-IGF2BP3/CBR3-AS1/miR-409-3p/CXCL1 axis, facilitating radioresistance in non-small-cell lung cancer. Journal of translational medicine 12 39962467
2024 RBM15 drives the progression of lung adenocarcinoma by regulating N6-methyladenosine-mediated LDHA mRNA stability. Life sciences 12 39406308
2010 Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57. Virology 12 20828777
2024 MLL1 promotes placental trophoblast ferroptosis and aggravates preeclampsia symptoms through epigenetic regulation of RBM15/TRIM72/ADAM9 axis. Biology direct 11 39709463
2025 Lactylation increases the stability of RBM15 to drives m6A modification in non-small-cell lung cancer cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 10 40135634
2024 RBM15 Knockdown Impairs the Malignancy of Cervical Cancer by Mediating m6A Modification of Decorin. Biochemical genetics 10 38429603
2024 RBM15 Drives Breast Cancer Cell Progression and Immune Escape via m6A-Dependent Stabilization of KPNA2 mRNA. Clinical breast cancer 10 39488447
2016 New hPSC-based human models to study pediatric Acute Megakaryoblastic Leukemia harboring the fusion oncogene RBM15-MKL1. Stem cell research 10 28412998
2024 RBM15-Mediated N6-Methyl Adenosine (m6A) Modification of EZH2 Drives the Epithelial-Mesenchymal Transition of Cervical Cancer. Critical reviews in eukaryotic gene expression 9 38842201
2023 Resveratrol affects ccRCC cell senescence and macrophage polarization by regulating the stability of CCNB1 by RBM15. Epigenomics 9 37909116
2025 RBM15-mediated m6A modification of XPR1 promotes the malignant progression of lung adenocarcinoma. Naunyn-Schmiedeberg's archives of pharmacology 8 39928150
2024 Identification of m6A modification patterns and RBM15 mediated macrophage phagocytosis in pancreatic cancer: An integrative analysis. Biochimica et biophysica acta. Molecular basis of disease 8 38878830
2024 RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells. Molecular medicine (Cambridge, Mass.) 7 39716068
1981 Neural differentiation in the OTT-6050 mouse teratoma. Production of a tumor fraction restricted to stem cells and neural cells after centrifugal elutriation. Virchows Archiv. A, Pathological anatomy and histology 7 7269228
2025 RBM15-MKL1 fusion protein promotes leukemia via m6A methylation and Wnt pathway activation. Blood 6 40435410
2024 The RNA m6A writer RBM15 contributes to the progression of esophageal squamous cell carcinoma by regulating miR-3605-5p/KRT4 pathway. Heliyon 6 38312624
2024 Overexpression of RBM15 modulated the effect of trophoblast cells by promoting the binding ability between YTHDF2 and the CD82 3'UTR to decrease the expression of CD82. Heliyon 6 38765115
2024 RBM15 activates glycolysis in M1-type macrophages to promote the progression of aortic aneurysm and dissection. International journal of medical sciences 6 39113895
2023 RBM15-Mediated m6A Modification of K17 Affects Keratinocytes Response to IL-17A Stimulation in Psoriasis. Annals of clinical and laboratory science 6 37625827
2025 RBM15 enhances paclitaxel resistance in triple-negative breast cancer by targeting m6A methylation of TNFSF9 and inducing polarization of tumor-associated macrophages to M2 phenotype. Hereditas 5 40830812
2024 RNA methylase RBM15 facilitates malignant progression of colorectal cancer through regulating E2F2 in an m6A modification-dependent manner. Journal of biochemical and molecular toxicology 5 39403943
2023 THE ROLE OF N6-METHYLADENOSINE METHYLTRANSFERASE RBM15 IN NONALCOHOLIC FATTY LIVER DISEASE. Shock (Augusta, Ga.) 5 38150369
2011 Ott's protein osmotic pressure of serum and interstitial fluid in chickens (Gallus gallus): effect of age and gender. The Journal of experimental biology 5 21270308
2025 RBM15, an m6A enzyme, suppresses NLRP3 inflammasome activation in rheumatoid arthritis through macrophage metabolism. Clinical and experimental rheumatology 4 40095631
2025 Role of N6-methyladenosine methyltransferase component RBM15 in cancer progression and its therapeutic potential. Discover oncology 4 40402374
2025 RBM15 promotes m6A methylation and stability of KLF6 mRNA to accelerate pyroptosis of retinal ganglion cells in early-stage diabetic retinopathy. Journal of molecular histology 3 40464812
2025 RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4. Hereditas 3 40682199
2025 Hypoxia-Induced Histone Lactylation Drives Cisplatin Resistance in Bladder Cancer by Promoting RBM15-Dependent m6A Methylation of IGFBP3. Research (Washington, D.C.) 3 41199784
2024 RBM15 Promotes High Glucose-Induced Lens Epithelial Cell Injury by Inducing PRNP N6-Methyladenine Modification During Diabetic Cataract. Current eye research 3 39206850
2024 RBM15-mediated the m6A modification of MAT2A promotes osteosarcoma cell proliferation, metastasis and suppresses ferroptosis. Molecular and cellular biochemistry 3 39527319
2025 RBM15 relies on m6A modification to inhibit UBE2C, alleviating hippocampal neuronal injury by limiting microglial inflammation. Molecular and cellular neurosciences 2 40049430
2025 lncSLERT Promotes Liver Metastasis in Colorectal Cancer by Down-Regulating HUNK Expression via RBM15-Mediated m6A Modification. OncoTargets and therapy 2 40371234
2025 RBM15 Mediated m6A Modification of SRSF1 Inhibits Cuproptosis in Non-Small Cell Lung Cancer by Mediating ATP7B Alternative Splicing. The Kaohsiung journal of medical sciences 2 40923717
2024 N6-Methyladenosine Modification of PERP by RBM15 Enhances the Tumorigenesis of Lung Adenocarcinoma via p53 Signaling Pathway. Molecular biotechnology 2 39556280
2024 RBM15 increase tumor-infiltrating CD4+ T cell in ESCC via modulating of PLOD3. American journal of cancer research 2 39659928
2025 m6A Methylation Regulator RBM15-Mediated Upregulation of ITGBL1 mRNA Stability Aggravates Colon Adenocarcinoma Progression by Remodeling the Tumor Microenvironment. The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 1 39840822
2025 RBM15-MKL1 fusion protein promotes leukemia via m6A methylation and WNT pathway activation. bioRxiv : the preprint server for biology 1 40060447
2025 Irradiation of 125I seeds blocks glycolysis in pancreatic cancer by inhibiting KLF5 m6A methylation through the suppression of RBM15. Experimental cell research 1 40147709
2025 RBM15-mediated metabolic reprogramming boosts immune response in colorectal cancer. Frontiers in immunology 1 40370450
2025 RBM15-mediated m6A methylation of Entpd1/CD39 regulates extracellular ATP hydrolysis and alleviates myocardial ischemia-reperfusion injury. International immunopharmacology 1 40614597
2025 Mechanism of RBM15 in high glucose-induced pyroptosis of renal tubular epithelial cells. Clinical and experimental nephrology 1 40658167
2025 RBM15 promotes COAD progression by regulating the m6A modification of TMC5. Hereditas 1 40883807
2025 RBM15 in diseases: Molecular mechanisms and clinical opportunities from RNA m6A methylation. Genes & diseases 1 41674656
2024 Examining spatiotemporal crowdsensing and caching for population-dynamic OTT content delivery. Scientific reports 1 38877123
2024 Mechanism of RBM15 in the malignant proliferation of colorectal cancer cells through regulating the stability of LncRNA FGD5-AS1 via m6A modification. Experimental cell research 1 39701357