Affinage

RAP1GAP

Rap1 GTPase-activating protein 1 · UniProt P47736

Length
663 aa
Mass
73.4 kDa
Annotated
2026-06-10
51 papers in source corpus 21 papers cited in narrative 21 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAP1GAP is a Rap1-specific GTPase-activating protein that terminates Rap1 signaling and thereby controls cell adhesion, migration, and receptor-driven ERK/MAPK output (PMID:10476970, PMID:20439492). Its catalytic domain (residues 75–416) stimulates GTP hydrolysis on Rap1 through a non-canonical catalytic asparagine, distinguishing it from the arginine-finger mechanism of Ras GAPs (PMID:15141215, PMID:1406653). Activity and abundance are tightly regulated by phosphorylation in a C-terminal regulatory region outside the catalytic domain: PKA phosphorylation (downstream of cAMP and D1 dopamine receptor signaling) inhibits GAP activity to raise Rap1-GTP (PMID:1587853, PMID:19218462), while GSK3β (Ser525) and mitotic PLK1 (Ser525, within a β-TrCP degron) and p34cdc2 (Ser484) phosphorylation route the protein toward proteasomal degradation (PMID:14660640, PMID:8048970, PMID:25329897). An N-terminal region (first 74 aa) binds heterotrimeric Gα subunits, coupling Rap1GAP to receptor signaling: Gαz binding blocks RGS-stimulated GTP hydrolysis and forms a stable Gαz–Rap1GAP–Rap1 complex (PMID:10593970), Gαo-GTP binds and activates Rap1GAP1 through a GoLoco/GPR motif (PMID:40615045), and Gi/o coupling translocates or degrades the rap1GAPII isoform to modulate Rap1 levels and drive ERK activation and neurite outgrowth (PMID:10476970, PMID:15657046). Through suppression of Rap1, Rap1GAP maintains E-cadherin-based cell–cell adhesion and β1 integrin-mediated attachment, and its loss enhances Src activity, Rac1-dependent migration, and invasion (PMID:20439492, PMID:23864657, PMID:24642466); consistent with a tumor-suppressive role, it is downregulated by oncogenic Ras/MEK/ERK signaling and by EZH2/miR-101-mediated epigenetic silencing in cancer (PMID:17646383, PMID:21532618). Rap1GAP also physically interacts with the RET receptor tyrosine kinase to restrain GDNF-induced signaling (PMID:20877310).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1992 High

    Establishing the modular architecture of Rap1GAP separated catalysis from regulation, defining where activity is generated versus where it is controlled.

    Evidence Deletion mutagenesis, in vitro GAP assays, and phosphopeptide mapping localizing the catalytic domain to aa 75–416 and PKA/cdc2 sites to the C-terminus

    PMID:1406653 PMID:1587853

    Open questions at the time
    • Functional consequence of each phosphorylation on activity not resolved here
    • No structural basis for catalysis yet
  2. 1994 Medium

    Mitotic phosphorylation at Ser484 by p34cdc2 raised the possibility of cell-cycle regulation of Rap1GAP, even though catalytic activity was unchanged.

    Evidence Cell-cycle synchronization with co-IP of cdc2/cyclin B1 and in vitro kinase assay on wild-type vs Ser484 mutant

    PMID:8048970

    Open questions at the time
    • No catalytic or abundance effect identified for Ser484 phosphorylation
    • Downstream functional role left open until later degron work
  3. 1999 High

    Discovery of Gα subunit binding via the N-terminus and of the rap1GAPII isoform connected Rap1GAP to GPCR signaling and to ERK/MAPK activation.

    Evidence Yeast two-hybrid, co-precipitation, subcellular fractionation, Rap1-GTP pull-down and adenylyl cyclase/ERK readouts for Gαz and Gαi binding

    PMID:10476970 PMID:10593970

    Open questions at the time
    • Nucleotide-state dependence of Gα binding not yet defined
    • How translocation alters local Rap1 pools mechanistically unresolved
  4. 2003 High

    GSK3β phosphorylation at Ser525 linking Rap1GAP stability to hormonal cAMP signaling showed that abundance, not only activity, is a regulated variable.

    Evidence In vitro kinase assay, GSK3β inhibitors, proteasome inhibitors, Ser525 mutagenesis in TSH-responsive cells

    PMID:14660640

    Open questions at the time
    • Identity of the ubiquitin ligase not defined here
    • Physiological scope beyond thyroid cells unclear
  5. 2004 High

    The crystal structure resolved the catalytic mechanism, revealing an asparagine thumb rather than the arginine finger used by Ras GAPs.

    Evidence X-ray crystallography of the catalytic domain with active-site mutagenesis, fluorescence titration, and stopped-flow kinetics

    PMID:15141215

    Open questions at the time
    • No co-structure with Rap1 in the published work
    • Structure of full-length regulatory regions not determined
  6. 2005 High

    Gαo/i-driven ubiquitination and degradation of rap1GAPII provided a mechanism for receptor-induced Rap1 activation and neurite outgrowth.

    Evidence Co-IP, ubiquitination assay, lactacystin, dominant-negative Rap1, siRNA, and pertussis toxin in Neuro-2A cells

    PMID:15657046

    Open questions at the time
    • Specific ligase mediating Gαo/i-directed degradation not identified
    • Relationship to GSK3β-driven turnover unresolved
  7. 2009 High

    PKA phosphorylation at Ser441/Ser499 inhibiting GAP activity placed Rap1GAP in dopamine-receptor-controlled regulation of dendritic spine morphology.

    Evidence Mass spectrometry, in vitro phosphorylation, and Rap1-GTP pull-down in striatal neurons after D1 receptor activation

    PMID:19218462

    Open questions at the time
    • Direct causal link between Rap1-GTP and spine size not fully dissected
    • Interplay with degradative phosphosites unaddressed
  8. 2010 Medium

    RET binding and adhesion-junction control defined Rap1GAP as a brake on receptor tyrosine kinase signaling and a maintainer of epithelial integrity.

    Evidence Yeast two-hybrid and endogenous Co-IP with RET (Tyr981), plus siRNA, immunofluorescence of junction proteins, and Src inhibition in colon carcinoma cells

    PMID:20439492 PMID:20877310

    Open questions at the time
    • Whether RET binding directly alters GAP activity not established
    • Mechanism connecting Rap to Src activation incompletely defined
  9. 2013 Medium

    Rap1GAP loss was shown to switch migratory mode by suppressing ROCK contractility and promoting Rac1-dependent mesenchymal motility.

    Evidence siRNA, Rap1-GTP pull-down, ROCK and Rac1 inhibitors, and live-cell migration tracking

    PMID:23864657

    Open questions at the time
    • Direct effectors linking Rap to ROCK/Rac1 not identified
    • In vivo relevance of mode-switching not tested
  10. 2014 High

    Mitotic PLK1 phosphorylation within a β-TrCP degron and in vivo podocyte studies established degron-driven turnover and the physiological consequence of Rap1GAP excess on integrin-mediated adhesion.

    Evidence In vitro kinase, ubiquitination, and Co-IP for PLK1/β-TrCP; podocyte-specific conditional knockouts and β1 integrin assays with human biopsy validation

    PMID:24642466 PMID:25329897

    Open questions at the time
    • Coordination of PLK1, GSK3β, and cdc2 phosphorylation across the cycle unresolved
    • How Rap1GAP elevation lowers active β1 integrin mechanistically incomplete
  11. 2021 Medium

    Identification of E6AP-mediated degradation and an AMPK interaction extended Rap1GAP regulation into viral oncogenesis and cardiomyocyte metabolic/autophagy control.

    Evidence Co-IP, MG132, E6AP siRNA in HPV+ cervical cancer; gain/loss-of-function with AMPK/AKT/mTOR Western blots, ROS, autophagy markers, and AMPK Co-IP in cardiomyocytes

    PMID:33936386 PMID:34952616

    Open questions at the time
    • Direct vs indirect nature of AMPK interaction not resolved
    • Whether E6AP recognizes a defined Rap1GAP degron unknown
  12. 2025 High

    Resolving that Gαo-GTP, not GDP, binds and activates Rap1GAP1 via a GoLoco/GPR motif tied the regulator to GNAO1 encephalopathy through conformation-dependent effector recognition.

    Evidence BioID proteomics, Co-IP, in vitro binding with nucleotide-locked Gαo mutants, GAP activity assays, and GoLoco/GPR motif mutagenesis

    PMID:40615045

    Open questions at the time
    • Downstream Rap1-dependent consequences in neurons not mapped
    • Relationship to the earlier Gαz/Gαi binding modes not reconciled

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many regulatory inputs—Gα nucleotide-state binding, multiple inhibitory and degradative phosphorylations, and distinct E3 ligases—are integrated to set Rap1-GTP levels in a given cell type remains unresolved.
  • No unified model of competing phosphorylation and degradation pathways
  • Cell-type-specific isoform usage (Rap1GAP1 vs rap1GAPII) not systematically mapped
  • Structural basis of Gα–Rap1GAP–Rap1 ternary complex undetermined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 4
Localization
GO:0005886 plasma membrane 2 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-1474244 Extracellular matrix organization 2
Partners
BTRCGNAO1GNAZGSK3BPLK1PRKAA1RAP1ARET

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Crystal structure of the catalytic domain of Rap1GAP at 2.9 Å resolution revealed that Rap1GAP uses a catalytic asparagine (not the arginine used by other GAPs) to stimulate GTP hydrolysis by Rap1. Mutational analysis, fluorescence titration, and stopped-flow kinetic assays confirmed the catalytic asparagine mechanism. X-ray crystallography, active-site mutagenesis, fluorescence titration, stopped-flow kinetics Nature High 15141215
1992 The catalytic domain of Rap1GAP was mapped to amino acids 75–416; the phosphorylation sites by cAMP-dependent kinase (Ser490, Ser499) and p34cdc2 (Ser484) are localized to the C-terminal region outside the catalytic domain. Deletion mutagenesis of cDNA constructs, purification of truncation mutants, in vitro GAP activity assays, phosphopeptide mapping, point mutagenesis Molecular and cellular biology High 1406653
1992 Rap1GAP is phosphorylated in vitro by cAMP-dependent kinase (~3 mol phosphate/mol) and p34cdc2 kinase (~2 mol phosphate/mol) at sites in the C-terminal 100-residue segment; dibutyryl-cAMP treatment of SK-MEL-3 cells promotes Rap1GAP phosphorylation in vivo at sites identical to the in vitro cAMP-dependent kinase sites. In vitro kinase assays with purified kinases, 32Pi labeling, comparative phosphopeptide mapping The Journal of biological chemistry High 1587853
1995 Tuberin (TSC2 product) immunoprecipitates contain Rap1GAP-like activity that specifically stimulates intrinsic GTPase of Rap1a but not Rap2, Ha-Ras, Rac, or Rho, establishing tuberin as a Rap1-specific GAP distinct from Rap1GAP. Immunoprecipitation of native tuberin followed by GTPase activity assay; bacterial and Sf9 cell-expressed C-terminal fragment assays The Journal of biological chemistry Medium 7608212
1999 A novel isoform of Rap1GAP, rap1GAPII, binds specifically to Gαi-family α-subunits; stimulation of the Gi-coupled m2-muscarinic receptor translocates rap1GAPII from cytosol to membrane, reduces GTP-bound Rap1, and thereby activates ERK/MAPK. Co-immunoprecipitation, subcellular fractionation, Rap1-GTP pull-down, ERK activation assays, receptor stimulation experiments Nature High 10476970
1999 Gαz binds Rap1GAP via the N-terminal 74 amino acids of Rap1GAP (a region distinct from the catalytic domain); this interaction blocks RGS-stimulated GTP hydrolysis of Gαz, attenuates Gαz-mediated adenylyl cyclase inhibition, and allows formation of a stable Gαz–Rap1GAP–Rap1 complex. Yeast two-hybrid, co-precipitation with purified recombinant proteins, adenylyl cyclase assay, N-terminal deletion analysis The Journal of biological chemistry High 10593970
2005 Gαo/i directly interacts with Rap1GAPII and targets it for ubiquitination and proteasomal degradation; this reduces Rap1GAP levels, activates Rap1, and drives CB1 cannabinoid receptor-induced neurite outgrowth in Neuro-2A cells. Proteasomal inhibitor lactacystin blocks Gαo/i-induced Rap1 activation and neurite outgrowth. Co-immunoprecipitation, ubiquitination assay, proteasomal inhibitor treatment (lactacystin), dominant-negative Rap1, siRNA knockdown, pertussis toxin treatment The Journal of biological chemistry High 15657046
2003 GSK3β phosphorylates Rap1GAP (at Ser525 identified by mutagenesis) and promotes its proteasome-mediated degradation; GSK3β inhibitors prevent phosphorylation and degradation of endogenous Rap1GAP. TSH/cAMP signaling stabilizes Rap1GAP, while TSH withdrawal leads to GSK3β-dependent Rap1GAP turnover. In vitro kinase assay (GSK3β on immunoprecipitated Rap1GAP), pharmacological GSK3β inhibitors, co-expression of GSK3β + Rap1GAP with proteasome inhibitors, point mutagenesis (Ser525) The Journal of biological chemistry High 14660640
2009 PKA phosphorylates Rap1GAP at Ser-441 and Ser-499 in striatal neurons in response to D1 dopamine receptor activation; this phosphorylation inhibits Rap1GAP GAP activity (increasing Rap1-GTP levels) and is associated with decreased dendritic spine head size. Mass spectrometry identification of PKA substrate, in vitro phosphorylation, Rap1-GTP pull-down in striatal neurons, D1 receptor activation, phospho-specific analysis Proceedings of the National Academy of Sciences of the United States of America High 19218462
1994 Rap1GAP is hyperphosphorylated during mitosis; p34cdc2 kinase co-immunoprecipitated from mitotic (but not interphase) HeLa cell lysates phosphorylates wild-type Rap1GAP but not a Ser484 mutant, indicating p34cdc2 phosphorylates Rap1GAP at Ser484 during mitosis. This phosphorylation does not affect GAP catalytic activity toward Rap1. Cell cycle synchronization, co-immunoprecipitation of cdc2/cyclin B1, in vitro kinase assay with wild-type and Ser484 mutant Rap1GAP, SDS-PAGE mobility shift Biochemical and biophysical research communications Medium 8048970
2014 PLK1 phosphorylates Ser525 within the 524DSGHVS529 degron of Rap1GAP, promoting its interaction with the β-TrCP ubiquitin ligase complex and subsequent proteasomal degradation during mitosis; PLK1 binds Rap1GAP via recognition of an SSP motif within Rap1GAP. Co-immunoprecipitation, in vitro kinase assay, ubiquitination assay, proteasome inhibitor treatment, mutagenesis of Ser525 and SSP motif PloS one High 25329897
2010 Rap1GAP physically interacts with the RET receptor tyrosine kinase via Tyr981 in RET's intracellular domain; endogenous Rap1GAP co-immunoprecipitates with RET in neural tissues; GDNF treatment enhances RET–Rap1GAP interaction; overexpression of Rap1GAP attenuates GDNF-induced ERK activation and neurite outgrowth, while Rap1GAP knockdown enhances them. Yeast two-hybrid, co-immunoprecipitation (endogenous proteins in neural tissue), RNAi knockdown, mutagenesis of RET Tyr981, neurite outgrowth assay, ERK phosphorylation assay Cell research High 20877310
2010 Silencing Rap1GAP in human colon carcinoma cells enhances Rap activity, impairs cell–cell adhesion (aberrant distribution of E-cadherin, β-catenin, p120-catenin), and enhances spreading/adhesion on collagen; silencing Rap expression rescues these defects; Src activity is increased in Rap1GAP-depleted cells and Src inhibition restores E-cadherin at cell–cell contacts. siRNA knockdown, Rap1-GTP pull-down assay, immunofluorescence of adherens junction proteins, Src kinase inhibitor treatment Molecular and cellular biology Medium 20439492
2007 Oncogenic Ras downregulates Rap1GAP expression via the Raf/MEK/ERK cascade in rat thyroid cells; restoring Rap1GAP inhibits Rap1 and Rac1 activity and blocks cell migration, invasion, DNA synthesis, and anchorage-independent growth. Ras transformation, MEK inhibitor treatment, Rap1-GTP/Rac1-GTP pull-down, siRNA knockdown, Rap1GAP re-expression, migration/invasion assays Molecular and cellular biology Medium 17646383
2013 Depletion of Rap1GAP in colon cancer cells enhances migration via increased endogenous Rap activity, suppresses ROCK-mediated contractility, and switches migratory mode to Rac1-dependent mesenchymal motility; Rac1 inhibition restores membrane blebbing and ROCK activity in Rap1GAP-depleted cells. siRNA knockdown, Rap1-GTP pull-down, ROCK inhibitor, Rac1 inhibitor, live-cell migration tracking, morphological analysis The Journal of biological chemistry Medium 23864657
2014 Podocyte-specific inactivation of both Rap1a and Rap1b (the targets of Rap1GAP) causes massive glomerulosclerosis; increased Rap1GAP expression in injured podocytes reduces active β1 integrin, leading to podocyte detachment; preventing RAP1GAP elevation maintains β1 integrin-mediated adhesion and prevents detachment. Insertional mutagenesis screen, podocyte-specific conditional knockout mice, β1 integrin activation assays, kidney biopsy immunostaining The Journal of clinical investigation High 24642466
2021 Degradation of Rap1GAP in HPV16/18-positive cervical cancer cells is mediated by the E6AP ubiquitin ligase via the proteasome pathway; co-immunoprecipitation showed E6AP binds Rap1GAP in HPV-positive cells; knockdown of E6AP shifts degradation from proteasomal to autophagy-mediated. Co-immunoprecipitation, MG132 proteasome inhibitor treatment, siRNA knockdown of E6AP, rapamycin-induced autophagy, Western blotting Infectious agents and cancer Medium 34952616
2025 Gαo-GTP (not GDP) binds and activates Rap1GAP1 via a GoLoco/GPR motif; specific residues in the GoLoco/GPR motif confer differential recognition of Gαo guanine-nucleotide-binding status; GNAO1 encephalopathy mutations in Gαo prevent it from attaining the conformation needed for Rap1GAP1 effector binding. Proximity-based proteomics (BioID) screen, co-immunoprecipitation, in vitro binding assays with GDP- and GTP-locked Gαo mutants, Rap1GAP1 activity assays, point mutagenesis of GoLoco/GPR motif The Journal of biological chemistry High 40615045
2022 In the subventricular zone niche, ADAM10 cleavage of JAMC increases Rap1GAP activity, which promotes neural stem cell transit from the apical to basal compartment and subsequent lineage progression. ADAM10 loss-of-function in vivo, NSC positioning assay, molecular pathway analysis linking JAMC processing to Rap1GAP activity Neural regeneration research Low 35535899
2011 EZH2 represses Rap1GAP by facilitating H3K27me3 at the Rap1GAP locus and promoting Rap1GAP promoter hypermethylation; loss of miR-101 leads to EZH2 upregulation and concomitant Rap1GAP downregulation in head and neck cancer. ChIP for H3K27me3, bisulfite methylation analysis, miR-101 overexpression/inhibition, Western blotting Oncogene Medium 21532618
2021 Rap1GAP overexpression in cardiomyocytes inhibits the AMPK/AKT/mTOR signaling pathway, exacerbates Ang II-induced cardiomyocyte hypertrophy, increases ROS generation, and inhibits autophagy; conversely, Rap1GAP knockdown activates AMPK/AKT/mTOR and reduces hypertrophy. Co-immunoprecipitation showed exogenous Rap1GAP interacts with AMPK. siRNA knockdown, adenoviral overexpression, AMPK/AKT/mTOR pathway Western blotting, ROS assay, autophagy markers, co-immunoprecipitation Oxidative medicine and cellular longevity Medium 33936386

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Identification of tuberin, the tuberous sclerosis-2 product. Tuberin possesses specific Rap1GAP activity. The Journal of biological chemistry 324 7608212
1999 Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with G alpha(i). Nature 198 10476970
2004 The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature 125 15141215
2005 Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPII. The Journal of biological chemistry 107 15657046
2011 The tumor suppressor gene rap1GAP is silenced by miR-101-mediated EZH2 overexpression in invasive squamous cell carcinoma. Oncogene 92 21532618
2006 Identification of a putative tumor suppressor gene Rap1GAP in pancreatic cancer. Cancer research 82 16424023
2010 Downregulation of Rap1GAP through epigenetic silencing and loss of heterozygosity promotes invasion and progression of thyroid tumors. Cancer research 79 20124489
1999 Functional interaction between Galpha(z) and Rap1GAP suggests a novel form of cellular cross-talk. The Journal of biological chemistry 78 10593970
2009 Down-regulation of Rap1GAP via promoter hypermethylation promotes melanoma cell proliferation, survival, and migration. Cancer research 74 19147557
2009 Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A controls Rap1 activity and dendritic spine morphology. Proceedings of the National Academy of Sciences of the United States of America 60 19218462
2006 Rap1GAP inhibits tumor growth in oropharyngeal squamous cell carcinoma. The American journal of pathology 54 16436672
1992 Localization of the rap1GAP catalytic domain and sites of phosphorylation by mutational analysis. Molecular and cellular biology 53 1406653
2010 Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion. Molecular and cellular biology 49 20439492
2007 Downregulation of Rap1GAP contributes to Ras transformation. Molecular and cellular biology 43 17646383
2014 Podocyte-specific RAP1GAP expression contributes to focal segmental glomerulosclerosis-associated glomerular injury. The Journal of clinical investigation 41 24642466
2012 Rap1GAP regulates renal cell carcinoma invasion. Cancer letters 41 22266190
2008 Loss of Rap1GAP in papillary thyroid cancer. The Journal of clinical endocrinology and metabolism 40 19066305
2003 Thyroid-stimulating hormone/cAMP and glycogen synthase kinase 3beta elicit opposing effects on Rap1GAP stability. The Journal of biological chemistry 35 14660640
1992 Phosphorylation of rap1GAP in vivo and by cAMP-dependent kinase and the cell cycle p34cdc2 kinase in vitro. The Journal of biological chemistry 29 1587853
2018 Downregulation of Rap1Gap: A Switch from DCIS to Invasive Breast Carcinoma via ERK/MAPK Activation. Neoplasia (New York, N.Y.) 24 30144784
2013 Tumor cell migration and invasion are enhanced by depletion of Rap1 GTPase-activating protein (Rap1GAP). The Journal of biological chemistry 23 23864657
2017 Rap1GAP inhibits tumor progression in endometrial cancer. Biochemical and biophysical research communications 21 28196746
2012 Rap1GAP alters leukemia cell differentiation, apoptosis and invasion in vitro. Oncology reports 21 22614916
2011 Histone deacetylase inhibitors upregulate Rap1GAP and inhibit Rap activity in thyroid tumor cells. Endocrine-related cancer 20 21367844
2012 RAP1GAP inhibits cytoskeletal remodeling and motility in thyroid cancer cells. Endocrine-related cancer 19 22696507
2021 Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress. Oxidative medicine and cellular longevity 16 33936386
2020 MiR-3121-3p promotes tumor invasion and metastasis by suppressing Rap1GAP in papillary thyroid cancer in vitro. Annals of translational medicine 15 33178761
2011 Rap1GAP impairs cell-matrix adhesion in the absence of effects on cell-cell adhesion. Cell adhesion & migration 15 21785277
2011 Inhibitory effects of Rap1GAP overexpression on proliferation and migration of endothelial cells via ERK and Akt pathways. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 14 22173489
2010 Rap1GAP interacts with RET and suppresses GDNF-induced neurite outgrowth. Cell research 14 20877310
2014 PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation. PloS one 10 25329897
2024 Rap1GAP exacerbates myocardial infarction by regulating the AMPK/SIRT1/NF-κB signaling pathway. Cellular signalling 9 38320624
2021 RAP1GAP Functions as a Tumor Suppressor Gene and Is Regulated by DNA Methylation in Differentiated Thyroid Cancer. Cytogenetic and genome research 9 34311462
1994 Phosphorylation of Rap1GAP during the cell cycle. Biochemical and biophysical research communications 9 8048970
2021 The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells. Infectious agents and cancer 8 34952616
1994 The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenetics and cell genetics 8 8275700
2022 ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling. Neural regeneration research 7 35535899
2022 Ovine RAP1GAP and rBAT gene polymorphisms and their association with tail fat deposition in Hu sheep. Frontiers in veterinary science 7 36090178
2018 Methylation level of Rap1GAP and the clinical significance in MDS. Oncology letters 7 30546468
2021 Downregulation of Rap1GAP Expression Activates the TGF-β/Smad3 Pathway to Inhibit the Expression of Sodium/Iodine Transporter in Papillary Thyroid Carcinoma Cells. BioMed research international 5 34840979
2004 Purification, crystallization and preliminary structural characterization of human Rap1GAP. Acta crystallographica. Section D, Biological crystallography 5 15039575
2025 GoLoco/GPR motif-dependent regulation of Rap1GAP1 by Gαo is disrupted by Gαo encephalopathy variants. The Journal of biological chemistry 4 40615045
2022 CpG Island Methylation of the Rap1Gap Gene in Medullary Thyroid Cancer. Archives of Iranian medicine 4 35429959
2021 Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway. Annals of translational medicine 4 35071437
2008 Expression of Rap1GAP in human myeloid disease following microarray selection. Genetics and molecular research : GMR 4 18551404
2009 Protein RAP1GAP in human myelodysplastic syndrome detected by flow cytometry and its clinical relevance. Zhongguo shi yan xue ye xue za zhi 3 19549374
2022 Simulated Microgravity Increases the Permeability of HUVEC Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2 Activation. International journal of molecular sciences 2 35054818
2009 Regulatory function and expression of rap1gap gene in hematopoietic cells-review. Zhongguo shi yan xue ye xue za zhi 2 19698268
2026 [The mechanism of Rap1GAP in ventricular hypertrophy and interstitial fibrosis induced by angiotensin Ⅱ]. Zhonghua xin xue guan bing za zhi 0 41866210
2024 Erratum: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway. Annals of translational medicine 0 42164041
2015 [The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 0 26304080

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