Affinage

Showing RAB6A — RAB6 is a alias.

RAB6A

Ras-related protein Rab-6A · UniProt P20340

Length
208 aa
Mass
23.6 kDa
Annotated
2026-06-10
100 papers in source corpus 50 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB6A is a Golgi-localized small GTPase that serves as a master organizer of multiple membrane trafficking steps, switching between GDP- and GTP-bound states to recruit a diverse array of effectors to the Golgi and to transport carriers (PMID:7798313, PMID:12738866). Its Golgi targeting requires geranylgeranylation and N-terminal sequences, with the nucleotide state governing both its prenylation and its membrane cycle through RabGDI extraction (PMID:8264642, PMID:8175798, PMID:8521955); the Ric1-Rgp1 complex acts as its GEF and GAPCenA as its GAP, the latter being shared with the kinetochore-associated functions of the protein (PMID:23091056, PMID:10202141). In its GTP-bound form RAB6 controls intra-Golgi retrograde transport and trafficking of trans-Golgi coated vesicles, and drives a COPI-independent, microtubule- and dynactin/dynein-dependent Golgi-to-ER retrograde pathway that recycles Golgi glycosylation enzymes (PMID:7798313, PMID:22335553, PMID:9050864, PMID:15483056). RAB6 achieves these movements by recruiting cytoskeletal effectors—dynactin (p150Glued), the dynein light chain DYNLRB1, BICD2/BICDR-1, and the kinesins Rabkinesin-6/KIF20A and KIF1C—coupling carriers to bidirectional microtubule-based transport (PMID:12401177, PMID:18044744, PMID:19019823, PMID:20360680, PMID:25821985, PMID:9438855). At the cell periphery RAB6 marks the majority of post-Golgi secretory carriers and, with kinesin-1, targets them to focal-adhesion-juxtaposed plasma-membrane hotspots defined by the cortical tether ELKS, where a sequential handoff to Rab8 via MICAL3 controls vesicle docking and fusion (PMID:17681140, PMID:31142554, PMID:21596566, PMID:32521280). The gene encodes two splice isoforms, Rab6A and Rab6A', differing by three amino acids, that perform non-overlapping functions: Rab6A predominates in Golgi-to-ER recycling while Rab6A' governs endosome-to-TGN retrograde transport and mitotic progression by regulating dynein/dynactin and the Mad2 spindle checkpoint at kinetochores (PMID:11071909, PMID:11839770, PMID:16536738, PMID:16395330). RAB6A is an essential gene whose loss is embryonic lethal in mice and whose secretory functions support pigmentation, T-cell receptor signaling, and neuronal development (PMID:26304202, PMID:28607494, PMID:29440364, PMID:35979738). Loss-of-function mutations in the RAB6 effector GORAB cause gerodermia osteodysplastica, linking RAB6-dependent secretory trafficking to connective-tissue homeostasis (PMID:18997784).

Mechanistic history

Synthesis pass · year-by-year structured walk · 27 steps
  1. 1993 High

    Established RAB6 as a component of the secretory machinery by showing a cytosolic p62-Rab6 complex required for budding of exocytic vesicles from the TGN.

    Evidence Cell-free vesicle budding assay with immunodepletion and competing peptides

    PMID:8349729

    Open questions at the time
    • Did not define the GTPase cycle or effector logic
    • p62 identity and direct biochemical contacts not resolved
  2. 1994 High

    Placed RAB6 functionally at the intra-Golgi transport step and defined the membrane-targeting determinants and GDI-dependent membrane cycle, answering where and how the GTPase acts.

    Evidence Overexpression of GTP/GDP mutants with transport assays; chimeric prenylation mutants; yeast complementation; in vitro membrane extraction with RabGDI

    PMID:7798313 PMID:8175798 PMID:8264642

    Open questions at the time
    • Effectors mediating transport not yet identified
    • Distinction between intra-Golgi and retrograde-to-ER roles not resolved
  3. 1995 Medium

    Showed that the nucleotide-bound conformation controls RAB6 prenylation and GDI-dependent extraction, linking the GTPase switch to the membrane attachment cycle.

    Evidence Conformational mutants in insect cells with Triton X-114 partitioning; two-hybrid plus membrane extraction with GDI beta

    PMID:7782346 PMID:8521955

    Open questions at the time
    • Single-lab/single-study for prenylation-conformation coupling
    • Novel two-hybrid interactor left unidentified
  4. 1997 High

    Revealed a microtubule-dependent Golgi-to-ER retrograde route by showing GTP-RAB6 redistributes Golgi enzymes to the ER, separating retrograde recycling from forward secretion.

    Evidence Rab6 mutant overexpression in HeLa with glycosylation/fractionation readouts and nocodazole microtubule disruption

    PMID:9050864

    Open questions at the time
    • Motor/tether machinery driving the route not yet defined
    • Relationship to COPI not yet established
  5. 1998 High

    Identified the first RAB6 motor effector, demonstrating that the GTPase couples membranes to microtubule motors via Rabkinesin-6.

    Evidence Two-hybrid, co-IP, GST pulldown, and dominant-negative effector-domain overexpression

    PMID:9438855

    Open questions at the time
    • Directionality of motor transport not resolved
    • Did not address dynein-based retrograde movement
  6. 1999 High

    Defined the COPI-independent nature of RAB6 retrograde transport and identified its cognate GAP, while linking RAB6 effectors to membrane tethering golgins.

    Evidence Live imaging of FP-Rab6 carriers and Shiga toxin transport; COPI-antibody microinjection; in vitro GAP assay for GAPCenA; golgin protein-blot binding

    PMID:10202141 PMID:10209123 PMID:10562278

    Open questions at the time
    • GEF still unknown at this stage
    • Golgin binding lacked reciprocal in-solution validation (idx 10)
  7. 2000 High

    Resolved that two splice isoforms differing by three amino acids carry distinct functions, and uncovered a mitotic role through the Rab6-binding kinesin in cytokinesis.

    Evidence Gene structure analysis, isoform overexpression with two-hybrid effector binding; Rab6-KIFL localization and inhibitory-antibody microinjection with time-lapse

    PMID:11060022 PMID:11071909

    Open questions at the time
    • Mechanism coupling RAB6 to the cytokinetic apparatus incomplete
    • Single-residue determinant of isoform divergence functionally probed but structural basis unknown
  8. 2001 High

    Assigned endosome-to-TGN retrograde transport specifically to the Rab6A' isoform with defined SNARE partners, separating isoform functions in distinct trafficking routes.

    Evidence Permeabilized-cell transport assay with isoform-specific and SNARE inhibition

    PMID:11839770

    Open questions at the time
    • Effectors specific to the Rab6A' endosome route not fully enumerated
    • Coordination with Golgi-to-ER route unresolved
  9. 2002 High

    Established RAB6 as the specificity factor recruiting dynactin to Golgi membranes and added new Golgi-recruited effectors, building the dynein/dynactin-based retrograde transport model.

    Evidence Pulldown/co-IP with recombinant Rabs and Golgi recruitment assays; two-hybrid plus recruitment for Rab6IP2A/B with Shiga toxin readout

    PMID:11929610 PMID:12401177

    Open questions at the time
    • Direct vs. adaptor-mediated dynactin contact not fully resolved
    • Rab6IP2 confirmed by single lab only
  10. 2003 High

    Provided direct evidence that active GTP-RAB6 resides on the Golgi and transport intermediates and shapes carrier geometry, validating the GTPase as conformationally active in situ.

    Evidence Conformation-specific intrabodies (GFP-tagged) for live and fixed imaging

    PMID:12738866

    Open questions at the time
    • Spatial regulation of GTP/GDP cycling on carriers not mapped
    • GEF/GAP localization relative to active pool not defined
  11. 2004 High

    Confirmed both isoforms drive dynactin-dependent Golgi-to-ER recycling and demonstrated by RNAi that RAB6 organizes the Golgi, integrating loss-of-function with overexpression evidence.

    Evidence Isoform-specific siRNA, GTP-mutant overexpression, dynamitin/Bicaudal-D dominant-negatives, immunofluorescence

    PMID:15483056

    Open questions at the time
    • Quantitative isoform contribution to recycling not separated here
    • Did not address mitotic functions
  12. 2006 High

    Dissected Rab6A' as the dominant retrograde isoform and uncovered a kinetochore function in inactivating the Mad2 checkpoint via dynein/dynactin dynamics, uncoupling trafficking and mitotic roles.

    Evidence Isoform-specific siRNA with Shiga toxin and cell-cycle readouts; dominant-negative and GAPCenA depletion with Mad2/p150Glued kinetochore staining; Ile62 mutagenesis

    PMID:16395330 PMID:16536738

    Open questions at the time
    • How a Golgi GTPase localizes activity to kinetochores not mechanistically resolved
    • GEF for the kinetochore pool unidentified
  13. 2007 High

    Defined RAB6's exocytic targeting role, its epistatic position upstream of retrograde tether complexes, and effector links bridging RAB6 to Rab11 and golgin TMF.

    Evidence Live imaging and siRNA for Rab6/kinesin-1 with ELKS colocalization; siRNA epistasis with ZW10/RINT-1 and COG; two-hybrid/FRET-FLIM for R6IP1; TMF binding and RNAi with immuno-EM

    PMID:17681140 PMID:17698061 PMID:17699596 PMID:17725553

    Open questions at the time
    • Mechanism by which ELKS captures carriers not yet defined
    • TMF and R6IP2 functional details confined to single labs
  14. 2008 High

    Provided quantitative biophysical parameters for effector binding, identified a dynein light chain partner, and linked RAB6 effector GORAB to human disease.

    Evidence SPR/fluorescence binding kinetics and GTPase modulation for PIST/BICD2/p150; two-hybrid/co-IP/pulldown for DYNLRB1; GORAB disease-mutation and interaction studies

    PMID:18044744 PMID:18997784 PMID:19019823

    Open questions at the time
    • DYNLRB1 nucleotide-state preferences differ across isoforms without structural explanation
    • GORAB mechanism in disease pathology not detailed (Medium)
  15. 2009 High

    Provided the structural basis for RAB6 effector promiscuity by solving the Rab6a(GTP)-Rab6IP1 complex and revealing conformational flexibility in its hydrophobic triad.

    Evidence X-ray crystallography at 3.2 Å with comparison to Rab6-GCC185

    PMID:19141279

    Open questions at the time
    • Structures of motor- and tether-effector complexes not solved
    • Isoform-specific structural differences not captured
  16. 2010 High

    Embedded RAB6 in a Rab33B->Rab6 retrograde cascade and identified BICDR-1 as a developmentally regulated effector restraining anterograde secretory transport in neurons.

    Evidence siRNA epistasis with GTP-Rab33B; co-IP, live imaging, siRNA, and zebrafish morpholino for BICDR-1/Kif1C

    PMID:20163571 PMID:20360680

    Open questions at the time
    • GEF connecting Rab33B to Rab6 not yet identified at this stage
    • Developmental switch controlling BICDR-1 levels not mechanistically defined
  17. 2011 High

    Defined a sequential RAB6-to-Rab8 handoff for exocytosis, showing RAB6 recruits Rab8 to carriers and MICAL3 monooxygenase activity remodels docking complexes for fusion.

    Evidence Live imaging, siRNA of Rab6/Rab8, co-IP, and MICAL3 catalytic-mutant expression with docking/fusion quantification

    PMID:21596566

    Open questions at the time
    • Molecular trigger for the Rab6-to-Rab8 conversion not defined
    • MICAL3 substrate during remodeling not identified
  18. 2012 High

    Identified the Ric1-Rgp1 GEF completing the RAB6 regulatory cycle and closing the Rab33B->Ric1/Rgp1->Rab6 cascade, and resolved the coated-vesicle defects of RAB6 loss ultrastructurally.

    Evidence In vitro nucleotide exchange with recombinant proteins, co-IP, siRNA with M6PR transport assay; electron tomography of Rab6-depleted Golgi

    PMID:22335553 PMID:23091056

    Open questions at the time
    • Spatial regulation of GEF activation on Golgi not mapped
    • Whether the cascade applies to all RAB6 routes unresolved
  19. 2013 High

    Extended RAB6's post-Golgi secretory role to immune effector output by showing both isoforms are needed for TGN-to-surface TNF carrier egress in activated macrophages.

    Evidence siRNA/shRNA depletion, dominant-negative mutants, live imaging of TGN carriers, secretion assays, EM

    PMID:23437303

    Open questions at the time
    • Cargo-selectivity of RAB6 carriers not addressed
    • Link to inflammatory signaling pathways not explored
  20. 2014 High

    Identified VPS13B/COH1 as a RAB6-dependent Golgi-recruited factor required for neurite outgrowth, broadening RAB6's role in neuronal membrane organization.

    Evidence siRNA, co-IP with Rab6 mutants, membrane fractionation, neurite outgrowth in primary neurons

    PMID:25492866

    Open questions at the time
    • Trafficking step served by COH1 not precisely defined
    • Direct vs. indirect RAB6-COH1 contact not fully resolved
  21. 2015 High

    Defined motor- and adaptor-level mechanisms—KIF1C autoinhibition by RAB6A binding and BICD2 stabilization of active RAB6—and refined GORAB disease mechanism, sharpening the transport machinery model.

    Evidence In vitro microtubule assays and cell studies for KIF1C; FRAP/reconstitution for BICD2; two-hybrid/mutagenesis for GORAB-RAB6/ARF5; RAB6A knockout MEFs with secretion and ricin assays plus embryonic lethality

    PMID:25821985 PMID:25962623 PMID:26000619 PMID:26304202

    Open questions at the time
    • How motor autoinhibition is relieved on carriers not resolved
    • GORAB-ARF5 binding interface in disease confirmed in single lab (Medium)
  22. 2017 High

    Demonstrated a physiological RAB6/ELKS secretory pathway delivering melanogenic cargo to melanosomes, with pigmentation defects in RAB6 KO mice establishing in vivo relevance.

    Evidence Live imaging, siRNA, RAB6 KO mouse, cargo tracking, melanosome maturation assays

    PMID:28607494

    Open questions at the time
    • Selectivity of RAB6 carriers for melanosomal cargo not mechanistically defined
    • Role of isoforms in melanocytes not separated
  23. 2018 High

    Connected RAB6 retrograde trafficking to adaptive immunity by showing RAB6/Syntaxin-16-dependent LAT recycling is required for immune synapse delivery and TCR signaling.

    Evidence siRNA in human cells, RAB6 KO mouse CD4+ T cells, LAT trafficking and TCR stimulation assays

    PMID:29440364

    Open questions at the time
    • How retrograde LAT routing achieves polarized synapse delivery not fully resolved
    • Effector mediating LAT capture not identified
  24. 2019 High

    Showed RAB6 spatially confines secretion to focal-adhesion-juxtaposed hotspots and uncovered an additional role in suppressing Cdc42 to restrain cell migration, broadening RAB6 beyond canonical secretion.

    Evidence RUSH synchronized secretion with live imaging and Rab6 inactivation; Cdc42 GTPase assay, co-IP with Cdc42/Trio, migration assays

    PMID:30830239 PMID:31142554

    Open questions at the time
    • Mechanistic coupling of secretion hotspots to focal adhesions not defined
    • Rab6-Cdc42/Trio interaction confirmed in single lab (Medium)
  25. 2020 High

    Established the molecular logic of carrier capture by showing ELKS1 tethers RAB6 cargo at presynaptic terminals through golgin-like mechanisms transferable to ectopic organelles.

    Evidence ELKS1 and Rab6 knockout/rescue, live imaging, mitochondria mistargeting experiments in neurons

    PMID:32521280

    Open questions at the time
    • Identity of the direct ELKS1-RAB6 tethering contact not defined
    • Generality across cell types beyond neurons not established
  26. 2021 High

    Showed RAB6-positive carriers deliver newly synthesized TrkB receptors into axons via combined kinesin-1/kinesin-3 activity, defining a directional anterograde transport mechanism in neurons.

    Evidence Microfluidic compartmental secretion assay, live imaging, kinesin-1/kinesin-3 knockdown/knockout, Rab6 colocalization

    PMID:33571451

    Open questions at the time
    • How motor handoff is regulated along the axon not resolved
    • Cargo-specificity of RAB6 axonal carriers not defined
  27. 2022 High

    Demonstrated RAB6 carriers move toward microtubule minus ends via dynein/LIS1 in apical radial glia to deliver apical determinants, linking RAB6 trafficking to neuroepithelial integrity in vivo.

    Evidence In situ live imaging, conditional RAB6 double knockout, LIS1 knockout, CRB3 localization, neuroepithelial integrity analysis

    PMID:35979738

    Open questions at the time
    • Switch between minus-end (dynein) and plus-end (kinesin) carrier transport not mechanistically resolved
    • Direct vs. effector-mediated dynein/LIS1 coupling not defined here

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RAB6 selects among its many competing effectors and trafficking routes in space and time, and what governs the directional switch between dynein-driven retrograde and kinesin-driven anterograde transport on individual carriers, remains unresolved.
  • No model integrates effector competition with carrier-specific motor selection
  • Spatial control of GEF/GAP activation on distinct membrane domains not mapped
  • Isoform-specific effector code not fully enumerated structurally

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 4 GO:0098772 molecular function regulator activity 4
Localization
GO:0005794 Golgi apparatus 4 GO:0005856 cytoskeleton 4 GO:0031410 cytoplasmic vesicle 4 GO:0005829 cytosol 3
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-9609507 Protein localization 4 R-HSA-1640170 Cell Cycle 3
Partners
BICD2DCTN1DYNLRB1ELKSGORABKIF1CKIF20ATMF

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 Rab6 controls intra-Golgi transport, specifically between cis/medial and late Golgi compartments. Overexpression of GTP-bound (Q72L) or wild-type Rab6 blocked transport between alpha-mannosidase II-positive and sialyl-transferase-positive Golgi compartments without affecting ER-to-cis/medial Golgi or TGN-to-plasma membrane steps. Transient overexpression of wild-type, GTP-bound (Q72L), and GDP-bound (T27N) Rab6 mutants in mouse L cells and HeLa cells; intracellular transport assays using secreted alkaline phosphatase (SEAP) and influenza hemagglutinin (HA) as markers The Journal of cell biology High 7798313
1994 Golgi apparatus localization of Rab6 requires geranylgeranylation (not farnesylation) and sequences in the N-terminal 71 amino acids including the effector domain. The C-terminal hypervariable domain is required to prevent prenylated/palmitoylated Rab6 from mislocalizing to the plasma membrane. Chimeric Ras-Rab proteins and Rab6 mutants with altered C-terminal lipid modifications expressed in mammalian cells; complementation of yeast ypt6 null mutants Molecular and cellular biology High 8264642
1994 Rab6 interaction with RabGDI requires the effector domain, loop3/beta3, and the hypervariable region; geranylgeranylation on CXC or CC motifs supports significantly better membrane extraction by RabGDI than farnesylation or palmitoylation. The effector domain is required for RabGDI binding but not for efficient processing by RabGGTase. In vitro membrane extraction assays with Rab6 mutants bearing various C-terminal lipid modifications; binding studies with recombinant proteins The Journal of biological chemistry High 8175798
1995 The nucleotide-bound conformation of Rab6 determines its posttranslational geranylgeranylation: only the GDP-bound form is isoprenylated and becomes membrane-bound in insect cells, whereas the GTP-bound form is not modified. Expression of GDP- and GTP-conformation point mutants of Rab6 in insect cells; Triton X-114 partitioning and cell fractionation to assess membrane association FEBS letters Medium 8521955
1995 Rab6 interacts with GDI beta (GDP-dissociation inhibitor beta isoform) in a GDP-dependent manner; GDI beta removes Rab6 from membranes. Rab6 also interacts with a novel, unidentified protein in its two-hybrid screen. Yeast two-hybrid screen using Rab6 as bait against mouse brain cDNA library; in vitro membrane extraction assay with recombinant GDI beta The Journal of biological chemistry Medium 7782346
1996 Rab6 is required for transport between cis and medial Golgi cisternae in a reconstituted cell-free Golgi transport assay. Anti-Rab6 antibodies and Fab fragments, as well as dominant-negative Rab6(N126I), inhibit transport and membrane fusion at the cisternal level. Cell-free reconstituted Golgi transport assay; inhibition with polyclonal antibodies, Fab fragments, and dominant-negative Rab6 mutant protein The Journal of biological chemistry High 8663167
1997 GTP-bound forms of Rab6 (wild-type and Q72L mutant) cause redistribution of Golgi resident proteins (e.g., beta-1,4-galactosyltransferase) into the ER and allow sialylated O-glycan addition to an ER-retained protein, phenocopying brefeldin A. This effect requires intact microtubules. GDP-bound Rab6 (T27N) does not cause redistribution but inhibits basal O-glycosylation. Overexpression of Rab6 mutants in HeLa cells; immunofluorescence, subcellular fractionation, glycosylation assays; microtubule depolymerization with nocodazole Proceedings of the National Academy of Sciences of the United States of America High 9050864
1998 Rab6 interacts in its GTP-bound form with Rabkinesin-6, a kinesin-like protein localized to the Golgi apparatus. The C-terminal domain of Rabkinesin-6 contains the Rab6-interacting domain. Overexpression of this C-terminal domain inhibits Rab6-GTP-dependent intracellular transport effects, identifying a molecular motor as an effector of Rab6. Yeast two-hybrid screen; co-immunoprecipitation; pulldown with GST-fusion proteins; dominant-negative overexpression assays in cells Science (New York, N.Y.) High 9438855
1999 Rab6 regulates a COPI-independent Golgi-to-ER retrograde transport pathway. FP-Rab6-positive transport carriers specifically accumulate Shiga toxin B-fragment (STB) during Golgi-to-ER transport. Overexpression of GDP-bound Rab6(T27N) inhibits Shiga holotoxin toxicity without affecting STB transport to the Golgi or Golgi morphology. Rab6/STB transport carriers are excluded from COPI-dependent recycling markers. Live-cell fluorescence microscopy of FP-Rab6 fusion; microinjection of COPI-blocking antibodies; T7 vaccinia-driven overexpression of Rab6 T27N; toxicity assays The Journal of cell biology High 10562278
1999 GAPCenA is a GTPase-activating protein (GAP) specifically active on Rab6 in vitro (and to lesser extent on Rab4 and Rab2). A minor pool of GAPCenA associates with the centrosome and forms complexes with cytosolic gamma-tubulin, suggesting a role in coordinating microtubule nucleation with Golgi dynamics. Identification by two-hybrid screen; in vitro GAP assay with recombinant proteins; immunofluorescence; cell fractionation; gamma-tubulin co-immunoprecipitation The EMBO journal High 10202141
1999 Golgins golgin-230/245/256 and golgin-97 target to the Golgi through a conserved C-terminal domain that preferentially binds Rab6. Mutations abolishing Golgi targeting also abrogate Rab6 binding. This domain defines a conserved family of Rab6-interacting coiled-coil proteins (golgins) likely involved in Rab6-regulated membrane tethering. Protein blot binding assay; mutagenesis; Golgi targeting assays in cells; sequence analysis across species Current biology : CB Medium 10209123
2000 Rab6A and Rab6A' are generated by alternative splicing of the RAB6A gene, differing in only three amino acids flanking the PM3 GTP-binding domain. Rab6A Q72L overexpression induces redistribution of Golgi proteins to the ER (retrograde), but Rab6A' Q72L does not. Rab6A' does not interact with Rabkinesin-6 but does interact with GAPCenA; one amino acid at position 87 (T vs A) underlies these functional differences. Gene structure analysis; GTP-binding assays; HeLa cell overexpression; immunofluorescence; yeast two-hybrid interaction assays for effector binding Molecular biology of the cell High 11071909
2000 Rab6-KIFL (Rab6-binding kinesin) was identified as a Rab6-interacting protein. Endogenous Rab6-KIFL localizes to the spindle midzone during anaphase and to the cleavage furrow/midbody during telophase. Microinjection of anti-Rab6-KIFL antibodies results in binucleate cells due to defective cleavage furrow formation, demonstrating a role for this Rab6 effector in cytokinesis. Yeast two-hybrid identification; immunofluorescence; microinjection of inhibitory antibodies; time-lapse microscopy The EMBO journal High 11060022
2001 Retrograde transport from early/recycling endosomes to the TGN requires the Rab6A' isoform (not Rab6A) along with specific SNARE complexes (syntaxin 6, syntaxin 16, Vti1a with VAMP3/cellubrevin and VAMP4). Rab6A has been previously implicated in Golgi-to-ER transport instead. Novel permeabilized cell transport assay; protein interaction studies; dominant-negative and antibody inhibition approaches The Journal of cell biology High 11839770
2002 Rab6 recruits the dynactin complex to Golgi membranes in a GTP-dependent and Rab6-specific manner. Other Golgi Rabs (tested) do not bind dynactin and cannot support its membrane recruitment, establishing Rab6 as a specificity factor for dynactin recruitment. Pulldown and co-immunoprecipitation assays with recombinant Rab proteins; Golgi membrane recruitment assays; comparison with other Golgi Rabs Current biology : CB High 12401177
2002 Two novel Rab6-interacting proteins, Rab6IP2A and Rab6IP2B (splice variants), are recruited to Golgi membranes in a Rab6:GTP-dependent manner. Overexpression of the Rab6-binding domain of Rab6IP2 partly inhibits retrograde transport of Shiga toxin B-subunit from plasma membrane to Golgi. Yeast two-hybrid screen; co-immunoprecipitation; Golgi recruitment assays; Shiga toxin transport assays with Rab6-binding domain overexpression Traffic (Copenhagen, Denmark) Medium 11929610
2003 Rab6 is in its GTP-bound conformation on the Golgi apparatus and on transport intermediates as demonstrated by GFP-tagged conformation-specific recombinant antibodies expressed intracellularly. The geometry of transport intermediates is modulated by Rab6 activity. Antibody phage display to generate GTP-conformation-specific Rab6 antibodies; GFP-tagging and intracellular expression for live-cell imaging; fixed-cell immunostaining Science (New York, N.Y.) High 12738866
2004 Both Rab6A and Rab6A' GTP-restricted mutants promote microtubule-dependent recycling of Golgi resident glycosylation enzymes to the ER with similar efficiency. Rab6-directed Golgi-to-ER recycling requires functional dynactin (inhibited by p50/dynamitin overexpression or C-terminal Bicaudal-D fragment). Reduced Rab6 via siRNA perturbs Golgi organization and delays Golgi-to-ER recycling. siRNA knockdown of individual Rab6 isoforms; overexpression of GTP-restricted mutants; p50/dynamitin dominant-negative overexpression; immunofluorescence Molecular biology of the cell High 15483056
2004 TMF/ARA160 is a Rab6-binding golgin that binds all three known Rab6 isoforms. Depletion of TMF by RNAi causes modest dispersal of Golgi membranes, suggesting a role in Golgi organization. Pulldown and binding assays between TMF and Rab6 isoforms; RNAi depletion with immunofluorescence analysis of Golgi morphology BMC cell biology Medium 15128430
2006 siRNA knockdown reveals that Rab6A' (but not Rab6A) is the major isoform regulating retrograde Shiga toxin transport and Golgi-associated protein recycling through the ER. Rab6A' is also required for cell cycle progression through mitosis; cells with impaired Rab6A' are blocked in metaphase with activated Mad2-spindle checkpoint. Ile62 is a key residue uncoupling Rab6A' functions in mitosis and retrograde trafficking. isoform-specific siRNA knockdown; Shiga toxin B-subunit transport assay; cell cycle analysis; mutagenesis of Ile62 Traffic (Copenhagen, Denmark) High 16536738
2006 Rab6A' GTPase activity at kinetochores regulates inactivation of the Mad2-spindle checkpoint. Impaired Rab6A' function (by dominant-negative expression or GAPCenA depletion) blocks cells in metaphase with activated Mad2 checkpoint and retains p150(Glued) (dynactin subunit) at kinetochores, suggesting Rab6A' regulates dynein/dynactin dynamics at kinetochores during mitosis. Dominant-negative Rab6A' expression; GAPCenA siRNA depletion; immunofluorescence for Mad2 and p150(Glued) at kinetochores; cell cycle analysis The EMBO journal High 16395330
2007 Rab6 marks exocytotic vesicles and, together with kinesin-1, drives processive microtubule-based transport to the cell periphery. Rab6 directs targeting of secretory vesicles to plasma membrane sites enriched in the cortical protein ELKS. Although Rab6 is not essential for secretion, it controls the spatial organization of exocytosis. Live-cell imaging of GFP-Rab6; siRNA knockdown of Rab6 and kinesin-1; co-localization of vesicles with ELKS-positive cortical sites; secretion assays Developmental cell High 17681140
2007 R6IP1 (Rab6-interacting protein 1) links Rab6 and Rab11 function: R6IP1 binds both Rab6 (targeting it to Golgi) and Rab11A (GTP-bound). Overexpression of R6IP1 promotes Rab11A-Rab6 interaction (FRET/FLIM) and causes pericentriolar accumulation of Rab11-positive recycling endosomes. R6IP1 function is also required during metaphase and cytokinesis. Yeast two-hybrid; co-immunoprecipitation; FRET/FLIM live-cell imaging; siRNA depletion; immunofluorescence; cell cycle analysis Traffic (Copenhagen, Denmark) High 17725553
2007 Rab6 depletion suppresses Golgi ribbon fragmentation/dispersal induced by ZW10/RINT-1 or COG complex inactivation (epistatic relationship). Dominant-negative Rab6 and C-terminal Bicaudal-D fragment (which interferes with dynactin/dynein) both suppress ZW10-knockdown-induced Golgi disruption. Rab6 therefore acts upstream in two separate retrograde tether-dependent Golgi trafficking pathways (ZW10/RINT-1 and COG). siRNA epistasis experiments; dominant-negative Rab6 expression; dominant-negative Bicaudal-D fragment; immunofluorescence; multiple Rab depletion combinations Molecular biology of the cell High 17699596
2007 TMF/ARA160 is functionally involved in two Rab6-dependent retrograde pathways: retrograde transport of Shiga toxin from endosomes to TGN, and retention of specific Golgi enzymes (GalNAc-T2 but not GalT) at the Golgi. TMF localizes to cisternal tips/budding structures at the Golgi as shown by immunoelectron microscopy. siRNA knockdown of TMF and Rab6; Shiga toxin transport assay; immunofluorescence; immunoelectron microscopy; chimeric protein domain swap experiments Experimental cell research High 17698061
2008 SCYL1BP1 (GORAB) localizes to the Golgi apparatus and interacts with Rab6, functioning as a golgin. Loss-of-function mutations in SCYL1BP1 cause gerodermia osteodysplastica, associating Rab6-dependent secretory pathway defects with age-related connective tissue changes. Identification of disease-causing mutations; subcellular localization studies; interaction assays between GORAB and Rab6 Nature genetics Medium 18997784
2008 DYNLRB1 (dynein light chain) specifically interacts with all three Rab6 isoforms and co-localizes at the Golgi. DYNLRB1 shows preferred association with GTP-bound Rab6A but GDP-bound Rab6A' and Rab6B, representing the first direct interaction identified between Rab6 and the dynein complex. Yeast two-hybrid; co-immunoprecipitation; pulldown with GTP/GDP-loaded Rab6 isoforms; immunofluorescence co-localization Cell motility and the cytoskeleton Medium 18044744
2008 Three Rab6a effectors (PIST, BicaudalD2, p150(Glued)) bind activated Rab6a through >15-kDa coiled-coil domains with Kd values in the high-nanomolar to low-micromolar range. BicaudalD2 and p150 binding moderately inhibits intrinsic Rab6a GTPase activity; PIST binding does not. Effectors bind in an apparent single-step reaction with relatively rapid on- and off-rates. In vitro biophysical binding assays (SPR, fluorescence); identification of minimal binding domains; GTPase activity assays with bound effectors The Journal of biological chemistry High 19019823
2009 Crystal structure of Rab6a(GTP) in complex with a 378-residue fragment of Rab6IP1 (containing RUN and PLAT domains) solved at 3.2 Å. The first and last alpha-helices of the RUN domain mediate binding to switch I, switch II, and the interswitch region of Rab6. Comparison with Rab6-GCC185 complex reveals conformational flexibility in the conserved hydrophobic triad of Rab6 that enables recognition of compositionally distinct effectors. X-ray crystallography; structure determination at 3.2 Å resolution; structural comparison with Rab6-GCC185 complex Structure (London, England : 1993) High 19141279
2010 BICDR-1 (Bicaudal-D-related protein 1) is a Rab6 effector that interacts with kinesin Kif1C and the dynein/dynactin complex, and accumulates Rab6 secretory vesicles in the pericentrosomal region of young neurons, restricting anterograde secretory transport and inhibiting neuritogenesis. BICDR-1 expression declines during development, permitting later anterograde transport required for neurite outgrowth. Co-immunoprecipitation; live-cell imaging; siRNA knockdown; zebrafish morpholino neural development assay; vesicle motility measurements The EMBO journal High 20360680
2010 Rab33B and Rab6 act sequentially in an intra-Golgi retrograde trafficking Rab cascade: Rab6 acts downstream of Rab33B (overexpression of GTP-Rab33B induces dissociation of Rab6 from Golgi membranes). Efficient GTP-Rab6-induced relocation of Golgi enzymes to the ER is Rab33B-dependent, but not vice versa. siRNA epistasis experiments; overexpression of GTP-restricted Rab mutants; immunofluorescence; Golgi morphology analysis; Shiga toxin transport assay Traffic (Copenhagen, Denmark) High 20163571
2011 Rab8A associates with exocytotic vesicles in a Rab6-dependent manner. Rab8A is required for docking and fusion (but not budding or motility) of exocytotic carriers. MICAL3 links Rab8A and ELKS at the cell cortex; MICAL3 monooxygenase activity is required for vesicle-docking complex remodeling and fusion. Rab6 and Rab8 thus cooperate sequentially in constitutive exocytosis. Live-cell imaging; siRNA knockdown of Rab6 and Rab8; co-immunoprecipitation; MICAL3 catalytic mutant expression; vesicle docking/fusion quantification Current biology : CB High 21596566
2012 The Ric1-Rgp1 complex is the guanine nucleotide exchange factor (GEF) for Rab6A in human cells. Both Ric1 and Rgp1 are required to catalyze nucleotide exchange on Rab6A and preferentially bind its GDP-bound form. Loss of Ric1 or Rgp1 destabilizes Rab6 and blocks retrograde transport of mannose-6-phosphate receptors to the Golgi. Rab33B-GTP binds the C-terminus of Ric1, establishing a Rab33B→Ric1/Rgp1→Rab6A cascade. In vitro nucleotide exchange assay with recombinant proteins; co-immunoprecipitation; siRNA knockdown; retrograde transport assay for mannose-6-phosphate receptors; domain-binding studies The Journal of biological chemistry High 23091056
2012 Rab6 depletion causes accumulation of trans-Golgi clathrin-coated and COPI-coated vesicles and an increase of >50% in Golgi cisternae number. Rab6 is essential for trafficking of these two morphological classes of coated vesicles at the trans-Golgi. siRNA knockdown of Rab6; electron microscopy and electron tomography; quantitative morphometric analysis Traffic (Copenhagen, Denmark) High 22335553
2013 Rab6A/A' are required for post-Golgi trafficking of TNF from TGN-derived tubular carriers to the cell surface in LPS-activated macrophages. Rab6 localizes to p230-positive TGN tubular carriers, and its depletion reduces carrier egress and partially reduces p230 membrane association. Both Rab6 isoforms are needed for macrophage Golgi organization and efficient post-Golgi TNF transport. siRNA and shRNA depletion; dominant-negative mutants; live-cell imaging of Rab6-GFP on TGN tubular carriers; secretion assays; electron microscopy PloS one High 23437303
2014 COH1 (VPS13B) Golgi localization depends on RAB6: RAB6A/A' knockdown prevents COH1 localization to the Golgi, constitutively inactive RAB6_T27N increases solubilization of COH1 from membranes, and constitutively active RAB6_Q72L preferentially co-immunoprecipitates with COH1. COH1 depletion in primary neurons impairs neurite outgrowth. siRNA knockdown; co-immunoprecipitation with RAB6 mutants; lipid membrane fractionation; neurite outgrowth assay in primary neurons The Journal of biological chemistry High 25492866
2015 KIF1C transports Rab6A vesicles and influences Golgi organization by binding Rab6A directly at both its motor domain and C-terminus. Rab6A binding to the KIF1C motor domain inhibits microtubule interaction in vitro and in cells. KIF1C depletion slows protein delivery to the cell surface, impairs vesicle motility, and causes Golgi fragmentation. KIF1C can protect Golgi from fragmentation independently of motor function when both Rab6A-binding sites are intact. In vitro microtubule interaction assay; pulldown; co-immunoprecipitation; siRNA knockdown; live-cell imaging; cargo delivery assay eLife High 25821985
2015 BICD2 stabilizes GTP-bound Rab6A on Golgi membranes: BICD2 knockdown reduces active Rab6A levels at the Golgi, and overexpression of C-terminal BICD2 decreases GFP-Rab6A exchange rate at Golgi (by FRAP). Rab6A and BICD2 jointly mediate COPI-independent Golgi-to-ER retrograde transport (Golgi tubule fusion with ER in BFA-treated cells). Golgi-targeting reconstitution in permeabilized cells; immunofluorescence; FRAP; siRNA knockdown; BFA-induced Golgi-to-ER transport assay Biochimica et biophysica acta High 25962623
2015 GORAB missense mutations (p.Ala220Pro and p.Ser175Phe) in the IGRAB domain disrupt RAB6 binding and Golgi targeting of GORAB. ARF5 (GTP-bound) also binds the same IGRAB domain, and the p.Ala220Pro mutation abrogates both RAB6 and ARF5 binding while p.Ser175Phe selectively impairs ARF5 binding. Yeast two-hybrid screening; immunofluorescence; Brefeldin A treatment; mutagenesis; protein interaction assays The Journal of investigative dermatology Medium 26000619
2015 RAB6A knockout mouse embryonic fibroblasts (MEFs) show altered Golgi morphology, decreased Golgi-associated levels of Rab6 effectors (Bicaudal-D and myosin II), delayed VSV-G secretion, and protection against ricin toxicity. RAB6A homozygous null mice die at early embryonic development, establishing RAB6A as an essential gene. Conditional RAB6A knockout using Cre-loxP/tamoxifen system; Western blot; immunofluorescence; secretion assays; ricin toxicity assays Biology of the cell High 26304202
2017 In melanocytes, the RAB6/ELKS-dependent secretory pathway directly transports and docks Golgi-derived carriers (carrying MART-1 and TYRP2/DCT) to melanosomes, controlling melanosome formation, maturation, and pigment synthesis. RAB6 KO mice display pigmentation defects. Live-cell imaging; siRNA knockdown; RAB6 KO mouse model; cargo tracking; melanosome maturation assays Nature communications High 28607494
2018 Rab6-dependent retrograde transport of LAT (linker for activation of T cells) through the Golgi-TGN is required for its polarized delivery to the immune synapse and for TCR-mediated T lymphocyte activation. This retrograde traffic also depends on Syntaxin-16. Rab6 KO CD4+ T lymphocytes show impaired TCR stimulation in vivo. siRNA knockdown of Rab6 and Syntaxin-16 in human cells; Rab6 KO mouse CD4+ T cells; LAT trafficking assays; immune synapse formation assays; TCR stimulation assays The Journal of experimental medicine High 29440364
2019 RAB6 and microtubules restrict protein secretion to focal-adhesion-juxtaposed hotspots at the cell surface. Most post-Golgi carriers are RAB6-positive regardless of cargo, and RAB6 inactivation leads to broad reduction of protein secretion. Synchronized secretion assay (RUSH system); live-cell imaging; siRNA/dominant-negative Rab6 inactivation; colocalization with focal adhesion markers The Journal of cell biology High 31142554
2019 Rab6 negatively regulates cell migration by interacting with Cdc42 and Trio (a GEF for Cdc42), thereby suppressing Cdc42 activity. Loss of Rab6 promotes actin protrusion formation and upregulates Cdc42 activity while downregulating myosin II phosphorylation. Rab6 KO/knockdown; Cdc42 GTPase activity assay; co-immunoprecipitation of Rab6 with Cdc42 and Trio; actin protrusion quantification; cell migration assays in vitro and in vivo Cellular and molecular life sciences : CMLS Medium 30830239
2020 ELKS1 captures Rab6-positive Golgi-derived vesicular cargo at presynaptic nerve terminals via Golgin-like mechanisms. Knockout and rescue experiments establish that ELKS1 captures Rab6 cargo; the capturing mechanism can be transferred to mitochondria by mistargeting ELKS1 or Rab6. ELKS1 and Rab6 knockout and rescue experiments; live-cell imaging; mitochondria mistargeting experiments; presynaptic vesicle assays in neurons Cell reports High 32521280
2021 Newly synthesized TrkB receptors traffic through the secretory pathway in Rab6-positive carriers and are directly delivered into the axon. The combined activity of kinesin-1 and kinesin-3 drives formation and anterograde transport of these TrkB/Rab6-positive secretory carriers beyond the proximal axon. Microfluidic compartmental devices; inducible secretion assay; live-cell imaging; siRNA/knockout of kinesin-1 and kinesin-3; Rab6 co-localization assays Developmental cell High 33571451
2022 Post-Golgi transport of RAB6+ vesicles in apical radial glia (aRG) cells occurs toward microtubule minus ends and depends on dynein and LIS1 (dynein activator). Double knockout of RAB6A/A' and RAB6B impairs apical localization of the apical determinant Crumbs3 (CRB3) and induces retraction of the apical process, leading to aRG delamination and ectopic division. These defects are phenocopied by LIS1 knockout. In situ subcellular live imaging; conditional RAB6 double knockout; LIS1 knockout; CRB3 localization assays; neuroepithelial integrity analysis EMBO reports High 35979738
1993 A cytosolic complex of p62 and Rab6 associates with TGN38/41 and is required for budding of exocytic vesicles from the TGN. Immunodepletion or competing peptides targeting p62, Rab6, or TGN38/41 cytoplasmic domains completely inhibit vesicle budding in a cell-free system. Co-immunoprecipitation from cell extracts; cell-free vesicle budding assay; immunodepletion; competing peptide inhibition; sizing column and velocity sedimentation The Journal of cell biology High 8349729
1999 Rab6 in Drosophila photoreceptors controls rhodopsin anterograde transport through the Golgi; GTPase-defective Rab6(Q71L) prevents maturation of rhodopsin beyond an immature 40-kDa form, depletes Rh1 and Rh3 levels, and causes retinal degeneration. Transgenic overexpression in Drosophila photoreceptors; Western blot analysis of rhodopsin forms; histological analysis; heat-shock rhodopsin pulse-chase The Journal of biological chemistry High 9685396
2000 In platelets, Rab6 is phosphorylated by protein kinase C (PKC) in a thrombin-stimulated manner. PKC phosphorylation of Rab6 increases GTP affinity ~3-fold, does not alter GTPase activity, and causes translocation of Rab6 from platelet particulate fractions to the cytosol. Metabolic [32P] labeling; PKC inhibitor (Ro-31-8220); cell fractionation; in vitro PKC phosphorylation of recombinant Rab6C; nucleotide binding affinity measurements The Biochemical journal High 10455022

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. The Journal of cell biology 446 11839770
1998 Interaction of a Golgi-associated kinesin-like protein with Rab6. Science (New York, N.Y.) 425 9438855
1999 Rab6 coordinates a novel Golgi to ER retrograde transport pathway in live cells. The Journal of cell biology 345 10562278
2007 Rab6 regulates transport and targeting of exocytotic carriers. Developmental cell 297 17681140
1994 The small GTP-binding protein rab6 functions in intra-Golgi transport. The Journal of cell biology 225 7798313
2000 The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis. The EMBO journal 180 11060022
2002 The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes. Current biology : CB 172 12401177
2011 Rab6, Rab8, and MICAL3 cooperate in controlling docking and fusion of exocytotic carriers. Current biology : CB 163 21596566
1997 GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America 151 9050864
1999 A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins. Current biology : CB 136 10209123
2002 Characterization of novel Rab6-interacting proteins involved in endosome-to-TGN transport. Traffic (Copenhagen, Denmark) 132 11929610
2010 Pericentrosomal targeting of Rab6 secretory vesicles by Bicaudal-D-related protein 1 (BICDR-1) regulates neuritogenesis. The EMBO journal 131 20360680
2008 Gerodermia osteodysplastica is caused by mutations in SCYL1BP1, a Rab-6 interacting golgin. Nature genetics 129 18997784
1999 Characterization of GAPCenA, a GTPase activating protein for Rab6, part of which associates with the centrosome. The EMBO journal 126 10202141
1993 A cytosolic complex of p62 and rab6 associates with TGN38/41 and is involved in budding of exocytic vesicles from the trans-Golgi network. The Journal of cell biology 124 8349729
2006 Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking. Traffic (Copenhagen, Denmark) 120 16536738
2009 Rab6 and Rab11 regulate Chlamydia trachomatis development and golgin-84-dependent Golgi fragmentation. PLoS pathogens 114 19816566
2003 Recombinant antibodies to the small GTPase Rab6 as conformation sensors. Science (New York, N.Y.) 105 12738866
2000 The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus. Journal of cell science 105 10893188
1995 Two-hybrid system screen with the small GTP-binding protein Rab6. Identification of a novel mouse GDP dissociation inhibitor isoform and two other potential partners of Rab6. The Journal of biological chemistry 102 7782346
2000 Alternative splicing of the human Rab6A gene generates two close but functionally different isoforms. Molecular biology of the cell 98 11071909
1996 Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. The Journal of biological chemistry 96 8663225
2004 Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'. Molecular biology of the cell 94 15483056
1993 Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins. Proceedings of the National Academy of Sciences of the United States of America 93 8356066
2019 RAB6 and microtubules restrict protein secretion to focal adhesions. The Journal of cell biology 92 31142554
2000 Multiple aspects of Rab protein action in the secretory pathway: focus on Rab3 and Rab6. Biochimie 88 10865125
2004 TMF is a golgin that binds Rab6 and influences Golgi morphology. BMC cell biology 85 15128430
2007 Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis. Molecular biology of the cell 79 17699596
2007 Rab6-interacting protein 1 links Rab6 and Rab11 function. Traffic (Copenhagen, Denmark) 79 17725553
2014 Cohen syndrome-associated protein COH1 physically and functionally interacts with the small GTPase RAB6 at the Golgi complex and directs neurite outgrowth. The Journal of biological chemistry 76 25492866
2010 Rab33b and Rab6 are functionally overlapping regulators of Golgi homeostasis and trafficking. Traffic (Copenhagen, Denmark) 72 20163571
2009 Structural basis for recruitment of Rab6-interacting protein 1 to Golgi via a RUN domain. Structure (London, England : 1993) 71 19141279
2009 The COG complex, Rab6 and COPI define a novel Golgi retrograde trafficking pathway that is exploited by SubAB toxin. Traffic (Copenhagen, Denmark) 67 19678899
2006 A role for the Rab6A' GTPase in the inactivation of the Mad2-spindle checkpoint. The EMBO journal 67 16395330
1999 Rab6 is phosphorylated in thrombin-activated platelets by a protein kinase C-dependent mechanism: effects on GTP/GDP binding and cellular distribution. The Biochemical journal 67 10455022
1996 Identification and localization of rab6, separation of rab6 from ERD2 and implications for an 'unstacked' Golgi, in Plasmodium falciparum. Molecular and biochemical parasitology 66 9010846
1998 Rab6 regulation of rhodopsin transport in Drosophila. The Journal of biological chemistry 65 9685396
2008 Rab6 family proteins interact with the dynein light chain protein DYNLRB1. Cell motility and the cytoskeleton 64 18044744
2006 Transport of ricin from endosomes to the Golgi apparatus is regulated by Rab6A and Rab6A'. Traffic (Copenhagen, Denmark) 64 16683916
2017 Routing of the RAB6 secretory pathway towards the lysosome related organelle of melanocytes. Nature communications 63 28607494
2012 Ric1-Rgp1 complex is a guanine nucleotide exchange factor for the late Golgi Rab6A GTPase and an effector of the medial Golgi Rab33B GTPase. The Journal of biological chemistry 63 23091056
2007 Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis. Development (Cambridge, England) 63 17329360
2012 Electron tomography reveals Rab6 is essential to the trafficking of trans-Golgi clathrin and COPI-coated vesicles and the maintenance of Golgi cisternal number. Traffic (Copenhagen, Denmark) 60 22335553
1994 Determination of structural requirements for the interaction of Rab6 with RabGDI and Rab geranylgeranyltransferase. The Journal of biological chemistry 60 8175798
1993 Rab6 is associated with a compartment that transports rhodopsin from the trans-Golgi to the site of rod outer segment disk formation in frog retinal photoreceptors. Journal of cell science 60 8308063
2015 miR-5100 promotes tumor growth in lung cancer by targeting Rab6. Cancer letters 58 25754817
1996 Differential effects of a Rab6 mutant on secretory versus amyloidogenic processing of Alzheimer's beta-amyloid precursor protein. The Journal of biological chemistry 58 8576122
2011 Characterization of Aspergillus nidulans RabC/Rab6. Traffic (Copenhagen, Denmark) 57 21226815
2007 Functional involvement of TMF/ARA160 in Rab6-dependent retrograde membrane traffic. Experimental cell research 57 17698061
2007 Rab6 is increased in Alzheimer's disease brain and correlates with endoplasmic reticulum stress. Neuropathology and applied neurobiology 55 17573808
2015 Rab6 regulation of the kinesin family KIF1C motor domain contributes to Golgi tethering. eLife 53 25821985
2012 RAB-6.2 and the retromer regulate glutamate receptor recycling through a retrograde pathway. The Journal of cell biology 53 22213799
2007 Rab6 and the secretory pathway affect oocyte polarity in Drosophila. Development (Cambridge, England) 53 17827179
2007 Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases. Cellular signalling 49 17716866
2013 Rab6a/a' are important Golgi regulators of pro-inflammatory TNF secretion in macrophages. PloS one 48 23437303
2013 Rab6 dependent post-Golgi trafficking of HSV1 envelope proteins to sites of virus envelopment. Traffic (Copenhagen, Denmark) 48 24152084
2018 Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. The Journal of experimental medicine 47 29440364
2020 Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis. Cell death & disease 45 33012781
2008 Biophysical analysis of the interaction of Rab6a GTPase with its effector domains. The Journal of biological chemistry 44 19019823
2016 Rab6 Is Required for Multiple Apical Transport Pathways but Not the Basolateral Transport Pathway in Drosophila Photoreceptors. PLoS genetics 43 26890939
2004 Rab6 membrane association is dependent of Presenilin 1 and cellular phosphorylation events. Brain research. Molecular brain research 43 14992812
1994 The effector domain of Rab6, plus a highly hydrophobic C terminus, is required for Golgi apparatus localization. Molecular and cellular biology 43 8264642
2009 Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex. The Journal of biological chemistry 42 19632984
2006 The Rab6 effector Bicaudal D1 associates with Chlamydia trachomatis inclusions in a biovar-specific manner. Infection and immunity 42 17101644
2012 Involvement of Rab6 in the regulation of phagocytosis against virus infection in invertebrates. Journal of proteome research 40 22928698
2007 Giantin interacts with both the small GTPase Rab6 and Rab1. Experimental cell research 40 17475246
2009 The localization of the Golgin GCC185 is independent of Rab6A/A' and Arl1. Cell 38 19703403
2020 ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals. Cell reports 36 32521280
2005 Rab6 interacts with the mint3 adaptor protein. Biological chemistry 35 16207088
2015 Phenotypic characterisation of RAB6A knockout mouse embryonic fibroblasts. Biology of the cell 34 26304202
2011 A role for the small GTPase Rab6 in assembly of human cytomegalovirus. Journal of virology 34 21411515
2022 RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination. EMBO reports 33 35979738
2002 Toxoplasma gondii Rab6 mediates a retrograde pathway for sorting of constitutively secreted proteins to the Golgi complex. The Journal of biological chemistry 32 12468555
2011 Rich regulates target specificity of photoreceptor cells and N-cadherin trafficking in the Drosophila visual system via Rab6. Neuron 31 21835342
2015 GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting. The Journal of investigative dermatology 30 26000619
2017 MiR-5100 increases the cisplatin resistance of the lung cancer stem cells by inhibiting the Rab6. Molecular carcinogenesis 27 29144562
2003 Evidence that the transport of ricin to the cytoplasm is independent of both Rab6A and COPI. Journal of cell science 27 12865434
2021 Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriers. Developmental cell 26 33571451
2016 The role of Rab6a and phosphorylation of non-muscle myosin IIA tailpiece in alcohol-induced Golgi disorganization. Scientific reports 26 27535804
2014 The trials and tubule-ations of Rab6 involvement in Golgi-to-ER retrograde transport. Biochemical Society transactions 26 25233431
2015 Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport. Biochimica et biophysica acta 25 25962623
2014 Distinct sets of Rab6 effectors contribute to ZW10--and COG-dependent Golgi homeostasis. Traffic (Copenhagen, Denmark) 23 24575842
2018 Rab6 promotes insulin receptor and cathepsin trafficking to regulate autophagy induction and activity in Drosophila. Journal of cell science 22 30111579
2012 Rab6 is a modulator of the unfolded protein response: implications for Alzheimer's disease. Journal of Alzheimer's disease : JAD 22 22124028
2012 Rab6 is required for the exocytosis of cortical granules and the recruitment of separase to the granules during the oocyte-to-embryo transition in Caenorhabditis elegans. Journal of cell science 22 22992455
2009 Structural aspects of Rab6-effector complexes. Biochemical Society transactions 22 19754447
1997 Small GTP-binding protein, Rab6, is associated with secretory granules in atrial myocytes. The American journal of physiology 22 9176151
2000 Identification of Rab6 as an N-ethylmaleimide-sensitive fusion protein-binding protein. The Biochemical journal 21 11062069
1996 Transport between cis and medial Golgi cisternae requires the function of the Ras-related protein Rab6. The Journal of biological chemistry 21 8663167
2016 Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation. Scientific reports 20 27030207
2002 WTH3, a new member of the Rab6 gene family, and multidrug resistance. Biochimica et biophysica acta 20 12007787
2016 ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles. Journal of cell science 18 27550519
2013 Targeting of the small GTPase Rab6A' by the Legionella pneumophila effector LidA. Infection and immunity 18 23569112
2020 Rab6 regulates recycling and retrograde trafficking of MR1 molecules. Scientific reports 17 33247182
2012 Rab6-mediated retrograde transport regulates inner nuclear membrane targeting of caveolin-2 in response to insulin. Traffic (Copenhagen, Denmark) 17 22607032
2018 The Biological Activity of AAV Vectors for Choroideremia Gene Therapy Can Be Measured by In Vitro Prenylation of RAB6A. Molecular therapy. Methods & clinical development 16 29707603
2005 Assay and functional properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis. Methods in enzymology 16 16473625
2019 Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activity. Cellular and molecular life sciences : CMLS 15 30830239
2017 Involvement of ARHGEF10, GEF for RhoA, in Rab6/Rab8-mediating membrane traffic. Small GTPases 15 28448737
1995 Nucleotide induced conformation determines posttranslational isoprenylation of the ras related rab6 protein in insect cells. FEBS letters 15 8521955

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