Affinage

POLR2G

DNA-directed RNA polymerase II subunit RPB7 · UniProt P62487

Length
172 aa
Mass
19.3 kDa
Annotated
2026-06-10
40 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

POLR2G (RPB7/hsRPB7) encodes the essential seventh subunit of RNA polymerase II, which forms an obligate heterodimer with RPB4 that contacts the Pol II core and is required for cell viability (PMID:8488730, PMID:9528765). The subcomplex sits in the floor of the DNA-binding cleft and stabilizes a minimal pre-initiation complex of promoter DNA, TBP, TFIIB and polymerase, acting at a post-recruitment step rather than in stable polymerase recruitment; RPB7's OB-fold mediates both nucleic-acid binding and the protein-protein contacts required for initiation, and the subunit promotes open-complex formation (PMID:9545247, PMID:11087726, PMID:17311916). RPB7 lines the RNA exit path (groove 1): the nascent transcript cross-links to RPB7 during early elongation and the contact is lost as the transcript lengthens, consistent with RPB7 marking the RNA exit channel (PMID:16327806, PMID:16282592). RPB7 stays associated with elongating polymerase through early elongation and dissociates upon recruitment of Ser2-phosphorylated CTD elongation factors during late elongation (PMID:17848138, PMID:23418395). Through these contacts RPB7 couples transcription to RNA processing by anchoring 3'-end/processing factors such as Nrd1/Seb1 to the polymerase (PMID:12907709, PMID:40038320), and it stabilizes the largest subunit RPB1 by recruiting the CTDP1 phosphatase and countering Cullin-3-mediated ubiquitylation (PMID:40038320). Beyond core transcription, RPB7 is a binding target for the oncogenic EWS-Fli1 fusion, whose EWS N-terminus contacts RPB7 to enhance EWS-chimera-driven transactivation (PMID:9704926), and for the tumor suppressor pVHL, which binds RPB7 through its beta-domain and targets it for ubiquitylation and proteasomal degradation, thereby limiting RPB7-driven VEGF promoter activation (PMID:12912922). RPB7 also represses transcription-coupled nucleotide excision repair via domain contacts overlapping with the Spt4/Spt5 pathway (PMID:37380080).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1993 High

    Established that RPB7 is an essential, RPB4-dependent component of Pol II, distinguishing it from the dispensable RPB4 and defining the architecture of the subcomplex's attachment to the core.

    Evidence Gene deletion and polymerase purification in S. cerevisiae

    PMID:8488730

    Open questions at the time
    • Did not define the molecular contacts anchoring RPB7 to the core
    • Did not establish the biochemical function of RPB7
  2. 1995 High

    Showed functional conservation by demonstrating the human ortholog assembles into and rescues yeast Pol II, validating yeast as a model for human RPB7 mechanism.

    Evidence Complementation, immunoprecipitation and two-hybrid in yeast expressing hsRPB7

    PMID:7579693

    Open questions at the time
    • Reduced affinity for yeast RPB4 left interface determinants undefined
    • No human-cell functional assay
  3. 1998 High

    Mapped the obligate RPB4–RPB7 heterodimer interface in human cells and placed the subcomplex in the floor of the DNA-binding cleft, where it stabilizes the pre-initiation complex.

    Evidence Reciprocal co-IP and domain mapping in mammalian cells; cryo-EM difference mapping and SPR in yeast

    PMID:9528765 PMID:9545247

    Open questions at the time
    • Mechanism linking cleft positioning to clamp closure inferred not proven
    • Atomic-resolution interface not yet resolved at this stage
  4. 1998 High

    Identified RPB7 as a direct target of the oncogenic EWS-Fli1 fusion, linking the core transcription subunit to oncogenic transactivation.

    Evidence Yeast two-hybrid, in vitro co-IP, nuclear-extract copurification and overexpression transactivation assay

    PMID:9704926

    Open questions at the time
    • Endogenous relevance in Ewing sarcoma cells not established
    • Mechanism by which RPB7 enhances chimera activity unresolved
  5. 2000 High

    Defined RPB7's OB-fold as the dual nucleic-acid-binding and initiation-competent element and showed the subcomplex acts at a post-recruitment step, not in stable polymerase loading.

    Evidence Purified-complex binding assays, OB-fold deletion mutagenesis, reconstituted transcription and template competition

    PMID:11087726

    Open questions at the time
    • Precise post-recruitment step (open complex vs clamp closure) not separated
    • In vivo confirmation of OB-fold requirement absent
  6. 2001 High

    Provided the first atomic view of the RPB7/RPB4 fold from the archaeal homolog, predicting an RNA-binding face that contacts the nascent transcript.

    Evidence X-ray crystallography of Methanococcus jannaschii subunits E and F

    PMID:11741548

    Open questions at the time
    • RNA contact was modeled, not observed
    • Archaeal divergence from eukaryotic Pol II left untested
  7. 2003 High

    Connected RPB7 to tumor-suppressor regulation by showing pVHL binds and targets RPB7 for ubiquitin-proteasome degradation, controlling RPB7-driven VEGF activation.

    Evidence Two-hybrid, co-IP, ubiquitylation and proteasome-inhibitor assays, luciferase reporter and ELISA in renal carcinoma cells

    PMID:12912922

    Open questions at the time
    • Whether degradation affects assembled Pol II or only free RPB7 unclear
    • Direct E3 ligase chemistry not reconstituted
  8. 2003 Medium

    Implicated RPB7 in coupling transcription to RNA processing by identifying a conserved interaction with the 3'-end formation factor Nrd1/Seb1, and identified RPB6 as a core attachment point for the subcomplex.

    Evidence Two-hybrid, in vitro direct binding and site-directed mutagenesis (Seb1/Nrd1); conditional Rpb6 mutant and interaction assays

    PMID:12697831 PMID:12907709

    Open questions at the time
    • No reciprocal co-IP for the Nrd1 interaction in mammalian cells
    • Functional consequence for processing not directly measured here
  9. 2005 High

    Localized the nascent RNA exit path to RPB7 and resolved the human heterodimer crystal structure with a mutagenesis-validated RNA-binding surface.

    Evidence Site-specific UV cross-linking on reconstituted elongation complexes; 2.7 Å human Rpb4/Rpb7 crystal structure with mutagenesis and EMSA

    PMID:16282592 PMID:16327806

    Open questions at the time
    • Functional role of the transient RNA contact in elongation not defined
    • Dynamics of contact loss at longer transcripts not mechanistically explained
  10. 2007 High

    Demonstrated a catalytic-stimulatory role by showing the archaeal RPB7 ortholog promotes open-complex (bubble) formation and stimulates core polymerase activity.

    Evidence Reconstituted archaeal RNA polymerase, permanganate footprinting and in vitro transcription

    PMID:17311916

    Open questions at the time
    • Generalization to eukaryotic Pol II not directly tested
    • Mechanistic link between RNA binding and bubble formation unresolved
  11. 2008 Medium

    Revised the initiation-only view by showing RPB7 remains with polymerase through early elongation and extends to late elongation under stress, indicating a role in stabilizing transcribing polymerase.

    Evidence TAP-ChIP with qPCR across transcribed regions in human cells

    PMID:17848138

    Open questions at the time
    • Single-lab ChIP without orthogonal occupancy method
    • Stress-induced retention mechanism undefined
  12. 2013 Medium

    Defined the elongation stage at which RPB7 leaves polymerase, showing dissociation coincides with Ser2-CTD phosphorylation and recruitment of elongation factors.

    Evidence Quantitative mass spectrometry with Rpb7-tagged Pol II immunoprecipitation

    PMID:23418395

    Open questions at the time
    • Trigger for dissociation not causally established
    • Reciprocity with reassociation during reinitiation untested here
  13. 2023 Medium

    Assigned RPB7 a repressive role in transcription-coupled nucleotide excision repair through distinct domain contacts, partly via the Spt4/Spt5 pathway and partly independently.

    Evidence Genetic epistasis with double mutants, TCR and UV-sensitivity assays in S. cerevisiae

    PMID:37380080

    Open questions at the time
    • Molecular mechanism of repression not biochemically defined
    • Relevance to human TCR not tested
  14. 2025 Medium

    Established RPB7 as a stabilizer of the largest subunit RPB1, acting through CTDP1 recruitment and antagonism of Cullin-3 ubiquitylation, and required for reinitiation and RNA processing.

    Evidence RPB7 depletion, co-IP, Cullin-3 ubiquitylation assay, reinitiation and RNA-processing assays in human cells

    PMID:40038320

    Open questions at the time
    • Not independently replicated
    • Direct CTDP1-RPB1 dephosphorylation step not reconstituted
    • Whether stabilization is constitutive or signal-regulated unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RPB7's RNA exit-channel positioning, elongation-stage dissociation/reassociation, and its roles in RPB1 stabilization and RNA processing are integrated into a single regulated transcription cycle remains unresolved.
  • No unified model linking RNA contact, CTD-phosphorylation-dependent dissociation, and reinitiation
  • Signal-dependent control of RPB7 abundance versus assembly not integrated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 3 GO:0005198 structural molecule activity 3 GO:0140110 transcription regulator activity 3 GO:0003677 DNA binding 1
Localization
GO:0005634 nucleus 2
Pathway
R-HSA-74160 Gene expression (Transcription) 4 R-HSA-8953854 Metabolism of RNA 2 R-HSA-73894 DNA Repair 1
Partners
CTDP1EWSR1NRD1POLR2APOLR2DPOLR2FRARAVHL
Complex memberships
RNA polymerase IIRPB4/RPB7 subcomplex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 RPB7 is essential for cell viability in S. cerevisiae; deletion of RPB7 is lethal, whereas deletion of RPB4 causes only conditional growth defects. RPB7 association with Pol II is dependent on RPB4, as polymerase purified from rpb4Δ cells also lacks RPB7. Gene deletion, yeast genetics, polymerase purification Yeast High 8488730
1995 Human hsRPB7 is a functional ortholog of yeast RPB7: it assembles into a complete 12-subunit yeast Pol II complex (shown by immunoprecipitation) and rescues the essential RPB7 deletion at moderate temperatures. However, hsRPB7 shows reduced affinity for yeast RPB4 compared to yeast RPB7, explaining failure to fully complement at temperature extremes. Complementation assay, immunoprecipitation of Pol II from yeast expressing hsRPB7, yeast two-hybrid Molecular biology of the cell High 7579693
1998 Oncogenic EWS-Fli1 specifically interacts with hsRPB7 (the human Pol II seventh subunit) via the EWS amino terminus; this interaction was identified by yeast two-hybrid and confirmed by in vitro immunoprecipitation. hsRPB7 copurifies with EWS-Fli1 but not with wild-type Fli1 from nuclear extracts. Overexpression of hsRPB7 specifically enhances gene activation by EWS-chimeric transcription factors. Yeast two-hybrid, in vitro immunoprecipitation, nuclear extract copurification, overexpression transactivation assay Oncogene High 9704926
1998 Human hsRPB4 strongly and specifically interacts with hsRPB7 in yeast and mammalian cells and copurifies with intact Pol II. Structure-function mapping defined the interaction interface between hsRPB4 and hsRPB7. hsRPB4 does not interact with yeast RPB7, showing species-specific divergence while retaining partial complementation of rpb4− yeast phenotypes. Yeast two-hybrid, coimmunoprecipitation in mammalian cells, polymerase copurification, truncation/deletion mapping Molecular and cellular biology High 9528765
1998 Cryo-EM difference mapping showed that Rpb4/Rpb7 form part of the floor of the DNA-binding cleft of yeast Pol II. Surface plasmon resonance demonstrated that Rpb4/Rpb7 stabilize a minimal pre-initiation complex containing promoter DNA, TBP, TFIIB and polymerase, suggesting a role in coupling DNA entry into the active center cleft to cleft closure. Cryo-EM 3D reconstruction, difference mapping, surface plasmon resonance The EMBO journal High 9545247
1999 Ligand-free RARα interacts with hsRPB7 through its DNA-binding domain (in the absence of retinoic acid); RA addition disrupts the interaction. Overexpressed hRARα (no RA) represses Pol II-dependent activators (AP-1, GR), and this repression is relieved by co-transfected hsRPB7, indicating that unliganded RARα sequesters hsRPB7 to repress transcription. Yeast two-hybrid, truncation analysis, transient transfection repression/rescue assays Endocrine Medium 10484292
1999 Rpb7 can associate with Pol II lacking Rpb4 (Pol IIΔ4) when overexpressed, demonstrated by two reciprocal coimmunoprecipitation experiments. This Rpb4-independent interaction supports transcription under mild stress, establishing that a major role of Rpb4 is to augment the affinity of Rpb7 for Pol II. Suppressor screen, reciprocal coimmunoprecipitation, transcription assays in rpb4Δ cells Molecular and cellular biology High 10082533
2000 The purified Rpb4/Rpb7 complex binds single-stranded DNA and RNA via an OB-fold motif in Rpb7. A small deletion in the OB-fold abolished nucleic acid binding without disrupting the Rpb4/Rpb7 complex or its association with Pol II, yet destroyed transcription activity. A separate OB-fold deletion blocked transcription but not nucleic acid binding, indicating the OB-fold mediates both nucleic acid binding and protein-protein interactions required for initiation. Template competition showed Rpb4/Rpb7 is not needed for stable polymerase recruitment but acts at a post-recruitment step. Purified complex binding assays (ssDNA/RNA), deletion mutagenesis, reconstituted transcription assay, template competition The Journal of biological chemistry High 11087726
2001 Crystal structure of the archaeal RPB7/RPB4 homologs (Methanococcus jannaschii subunits E and F) was determined, revealing an elongated two-domain structure for subunit E with two potential RNA-binding motifs, and subunit F wrapping around subunit E at the domain interface. A model was proposed in which the RNA-binding face of RPB7 contacts the nascent RNA transcript. X-ray crystallography Molecular cell High 11741548
2003 pVHL directly binds to hsRPB7 through its beta-domain; naturally occurring beta-domain mutations decrease this interaction. Introduction of wild-type pVHL into VHL-mutant renal carcinoma cells promotes ubiquitylation and proteasomal degradation of hsRPB7, reduces its nuclear accumulation, and suppresses hsRPB7-induced VEGF promoter transactivation and VEGF secretion. Yeast two-hybrid, co-immunoprecipitation, ubiquitylation assay, proteasome inhibitor experiments, luciferase reporter assay, ELISA The EMBO journal High 12912922
2003 In fission yeast, Rpb7 directly interacts in vitro with Seb1 (the S. pombe homolog of Nrd1, an RNA-binding protein for 3′-end formation of snRNA/snoRNA). Two-hybrid and in vitro binding identified Glu166/Asp167 near the Rpb7 C-terminus as critical for the interaction. S. cerevisiae Rpb7 also interacts with Nrd1, showing evolutionary conservation. This suggests Rpb7 anchors an RNA-processing factor to the Pol II apparatus. Two-hybrid screen, in vitro direct binding, site-directed mutagenesis Nucleic acids research Medium 12907709
2003 A conditional mutation in the shared Rpb6 subunit (Q100R) causes selective loss of Rpb4 and Rpb7 from purified Pol II. Interaction experiments support a direct association between Rpb6 and Rpb4, identifying Rpb6 as one contact point through which the Rpb4/Rpb7 subcomplex associates with the Pol II core. Genetic screen, polymerase purification, interaction experiments (pulldown/co-IP) Molecular and cellular biology Medium 12697831
2005 UV cross-linking of uridine analogs in the first 6 nt of nascent RNA demonstrates that the emerging transcript contacts Rpb7 in Pol II elongation complexes carrying 26–32 nt transcripts; this interaction is greatly reduced at 41–43 nt and absent at 125 nt, establishing groove 1 (pointing toward Rpb4/Rpb7) as the nascent RNA exit path. UV cross-linking with uridine analogs on reconstituted elongation complexes Nature structural & molecular biology High 16327806
2005 Crystal structure of the human Rpb4/Rpb7 heterodimer was determined at 2.7 Å. Site-directed mutagenesis of conserved surface residues in Rpb7 (and archaeal subunit E), combined with EMSA RNA-binding assays, identified an elongated surface spanning the OB-fold B4–B5 loop and extending to the N-terminal domain as the RNA-binding interface. X-ray crystallography, site-directed mutagenesis, EMSA Nucleic acids research High 16282592
2005 Fission yeast Rpb7 has a specific role in transcription of centromeric pre-siRNA precursors required for RNAi-directed heterochromatin formation. The rpb7-G150D point mutation specifically impairs centromeric pre-siRNA transcription (and thus RNAi silencing) without equivalent effects seen with other Pol II subunit mutants. Point mutation analysis, centromeric transcription assays, RNAi/heterochromatin reporter assays Genes & development Medium 16204182
2005 In fission yeast, Rpb7 interacts with both GAPDH and actin. GAPDH was identified as an Rpb7-binding protein by two-hybrid screen and confirmed by affinity purification from S. pombe extract using an Rpb4/Rpb7-coupled column. Actin was identified as a Pol II-associated protein and shown to interact with Rpb7. Two-hybrid screen, affinity purification (Rpb4/Rpb7-coupled column pull-down) FEBS letters Low 15620689
2005 Mutations in the N-terminal RNP-like domain of S. cerevisiae Rpb7 impair stress responses (high-temperature growth, sporulation) and increase Rpb7's dependence on Rpb4 for Pol II interaction. RNA polymerase pulldown assays revealed two crucial interaction points between the Rpb4/Rpb7 subcomplex and the core: the N-terminal RNP-like domain of Rpb7 and the partially ordered N-terminus of Rpb4 (contacting Rpb2). Mutagenesis, RNA polymerase pulldown assay, complementation analysis The Journal of biological chemistry Medium 18056993
2007 In Pyrococcus furiosus, the archaeal RPB7 ortholog E' (subunit E') stimulates Pol II core enzyme activity at low temperatures and promotes open complex formation (bubble formation at multiple positions in the transcription bubble), as shown by permanganate footprinting of reconstituted transcription complexes. Subunit F did not significantly contribute to catalytic activity. Reconstitution of archaeal RNA polymerase, permanganate footprinting, in vitro transcription at varying temperatures The Journal of biological chemistry High 17311916
2008 ChIP experiments in live human cells show that Rpb7 remains associated with Pol II during early elongation (not solely at initiation as previously believed), and under stress conditions Rpb7 association extends to late elongation stages, suggesting a role in stabilizing transcribing polymerase throughout the transcription cycle. TAP-ChIP (chromatin immunoprecipitation) with quantitative PCR across promoter-proximal and downstream transcribed regions in human cells The Biochemical journal Medium 17848138
2008 Pull-down assays confirmed direct in vitro interaction between the first 57 residues of the EWS N-terminal activation domain (EAD) and hsRPB7, forming a stable complex. In vitro pull-down assay with recombinant proteins Molecular biology reports Low 18607770
2013 Quantitative proteomics demonstrated that Rpb4/Rpb7 dissociate from Pol II upon interaction with transcriptional elongation-associated proteins recruited to the hyperphosphorylated CTD (Ser2-P form). Pol II isolated through Rpb7 is depleted in Ser2-CTD phosphorylation, indicating Rpb4/7 are dispensable during specific elongation stages. Quantitative proteomics (mass spectrometry), immunoprecipitation via Rpb7-tagged Pol II Molecular & cellular proteomics Medium 23418395
2023 Rpb7 functions as a repressor of transcription-coupled nucleotide excision repair (TCR) in S. cerevisiae. The Rpb7 region interacting with the KOW3 domain of Spt5 represses TCR through a common mechanism with Spt4/Spt5. The Rpb7 regions contacting Rpb4 and/or core RNAPII repress TCR largely independently of Spt4/Spt5, as shown by synergistic enhancement of TCR derepression when combined with spt4Δ. Genetic epistasis (double mutants), TCR assays at specific gene loci, UV sensitivity assays The Journal of biological chemistry Medium 37380080
2025 RPB7 stabilizes RPB1 (the largest Pol II subunit): depletion of RPB7 destabilizes RPB1 via the E3 ubiquitin ligase Cullin 3. Stabilization depends on the loop regions of RPB7, CDK9, the CTD and linker region of RPB1. RPB7 also interacts with the phosphatase CTDP1, which is required for RPB1 stability. RPB7 is essential for Pol II reinitiation, engages RNA-processing factors, and localizes to the RNA exit channel; its absence compromises RNA processing. RPB7 depletion experiments, co-immunoprecipitation (RPB7-CTDP1 interaction), ubiquitylation assay (Cullin 3), reinitiation assays, RNA processing assays, localization data Nature communications Medium 40038320
2005 Mapping of the Rpb4–Rpb7 interaction interface by two-hybrid analysis with truncation mutants showed that both the amino- and carboxy-terminal domains of Rpb7 are required for interaction with Rpb4, and that N-terminal deletions of Rpb4 (beyond 49 residues) progressively abolish interaction with Rpb7. Yeast two-hybrid with truncation mutants, computational structural analysis Biochemical and biophysical research communications Low 15913559

Source papers

Stage 0 corpus · 40 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing. Genes & development 199 16204182
1998 Oncogenic EWS-Fli1 interacts with hsRPB7, a subunit of human RNA polymerase II. Oncogene 117 9704926
2001 Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex. Molecular cell 90 11741548
2004 Rpb4 and Rpb7: subunits of RNA polymerase II and beyond. Trends in biochemical sciences 87 15544954
2005 RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit. Nature structural & molecular biology 86 16327806
2003 Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein. The EMBO journal 80 12912922
2000 Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation. The Journal of biological chemistry 73 11087726
1993 RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability. Yeast (Chichester, England) 71 8488730
1995 Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology. Molecular biology of the cell 65 7579693
2007 The RPB7 orthologue E' is required for transcriptional activity of a reconstituted archaeal core enzyme at low temperatures and stimulates open complex formation. The Journal of biological chemistry 63 17311916
2005 Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II. Nucleic acids research 59 16282592
1998 Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Molecular and cellular biology 57 9528765
1998 Structure of wild-type yeast RNA polymerase II and location of Rpb4 and Rpb7. The EMBO journal 56 9545247
1999 Rpb7 can interact with RNA polymerase II and support transcription during some stresses independently of Rpb4. Molecular and cellular biology 53 10082533
2003 Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. Nucleic acids research 46 12907709
2017 Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7. Biotechnology for biofuels 45 28515784
2002 The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits. Proceedings of the National Academy of Sciences of the United States of America 43 12407181
2003 An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP. Molecular and cellular biology 38 12482973
1994 C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7. Molecular and cellular biology 38 8065349
2013 Quantitative proteomics demonstrates that the RNA polymerase II subunits Rpb4 and Rpb7 dissociate during transcriptional elongation. Molecular & cellular proteomics : MCP 37 23418395
2005 Rpb4 and Rpb7: a sub-complex integral to multi-subunit RNA polymerases performs a multitude of functions. IUBMB life 31 16036568
2003 Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III. Molecular and cellular biology 31 12697831
2008 Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription. The Biochemical journal 28 17848138
2005 Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit. FEBS letters 28 15620689
2012 Rpb4 and Rpb7: multifunctional subunits of RNA polymerase II. Critical reviews in microbiology 19 22917057
2011 Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7. Molecular and biochemical parasitology 14 21816181
2009 RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei. EMBO reports 13 19165144
2008 In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II hsRPB7. Molecular biology reports 9 18607770
1999 Ligand-free RAR can interact with the RNA polymerase II subunit hsRPB7 and repress transcription. Endocrine 9 10484292
1999 An allele of the yeast RPB7 gene, encoding an essential subunit of RNA polymerase II, reduces cellular resistance to the antitumor drug bleomycin. Biochemistry and cell biology = Biochimie et biologie cellulaire 9 10546901
2007 Unstructured N terminus of the RNA polymerase II subunit Rpb4 contributes to the interaction of Rpb4.Rpb7 subcomplex with the core RNA polymerase II of Saccharomyces cerevisiae. The Journal of biological chemistry 8 18056993
2004 Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation. Nucleic acids research 8 14704357
2001 Suprabasal overexpression of the hsRPB7 gene in psoriatic epidermis as identified by a reverse transcriptase-polymerase chain reaction differential display model comparing psoriasis plaque tissue with peritonsillar mucosa. The American journal of pathology 7 11159173
1997 Human gene for the RNA polymerase II seventh subunit (hsRPB7): structure, expression and chromosomal localization. Biochimica et biophysica acta 7 9256063
2019 A comparative study of the proteome regulated by the Rpb4 and Rpb7 subunits of RNA polymerase II in fission yeast. Journal of proteomics 4 30862564
2014 Modulating the level of the Rpb7 subunit of RNA polymerase II affects cell separation in Schizosaccharomyces pombe. Research in microbiology 4 25530312
2019 Genome-wide transcriptional response to altered levels of the Rpb7 subunit of RNA polymerase II identifies its role in DNA damage response in Schizosaccharomyces pombe. FEMS yeast research 3 30395243
2005 Mapping the interaction site of Rpb4 and Rpb7 subunits of RNA polymerase II in Saccharomyces cerevisiae. Biochemical and biophysical research communications 3 15913559
2025 CTDP1 and RPB7 stabilize Pol II and permit reinitiation. Nature communications 2 40038320
2023 Rpb7 represses transcription-coupled nucleotide excision repair. The Journal of biological chemistry 2 37380080

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